Wandi Liu, Chaohui Yan, Ruimin Li, Guanyu Chen, Xinqi Wang, Yingqiang Wen, Chaohong Zhang, Xiping Wang, Yan Xu, Yuejin Wang
{"title":"VqMAPK3/VqMAPK6、VqWRKY33和VqNSTS3构成了增强葡萄对白粉菌抗性的调控节点。","authors":"Wandi Liu, Chaohui Yan, Ruimin Li, Guanyu Chen, Xinqi Wang, Yingqiang Wen, Chaohong Zhang, Xiping Wang, Yan Xu, Yuejin Wang","doi":"10.1093/hr/uhad116","DOIUrl":null,"url":null,"abstract":"<p><p>Grapevine powdery mildew is caused by <i>Erysiphe necator</i>, which seriously harms grape production in the world. Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew. A novel <i>VqNSTS3</i> was identified and cloned from Chinese wild <i>Vitis quinquangularis</i> accession Danfeng-2. The novel <i>VqNSTS3</i> was transferred into susceptible 'Thompson Seedless' by <i>Agrobacterium</i>-mediated transformation. The transgenic plants showed resistance to the disease and activated other resistance-related genes. <i>VqNSTS3</i> expression in grapevine is regulated by VqWRKY33, and which binds to TTGACC in the <i>VqNSTS3</i> promoter. Furthermore, VqWRKY33 was phosphorylated by VqMAPK3/VqMAPK6 and thus led to enhanced signal transduction and increased <i>VqNSTS3</i> expression. <i>Pro</i><i>VqNSTS3::VqNSTS3</i>-GFP of transgenic <i>VqNSTS3</i> in <i>Arabidopsis thaliana</i> was observed to move to and wrap the pathogen's haustoria and block invasion by <i>Golovinomyces cichoracearum</i>. These results demonstrate that stilbene accumulation of novel <i>VqNSTS3</i> of the Chinese wild <i>Vitis quinquangularis</i> accession Danfeng-2 prevented pathogen invasion and enhanced resistance to powdery mildew. Therefore, <i>VqNSTS3</i> can be used in generating powdery mildew-resistant grapevines.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 7","pages":"uhad116"},"PeriodicalIF":7.6000,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541564/pdf/","citationCount":"0","resultStr":"{\"title\":\"VqMAPK3/VqMAPK6, VqWRKY33, and <i>VqNSTS3</i> constitute a regulatory node in enhancing resistance to powdery mildew in grapevine.\",\"authors\":\"Wandi Liu, Chaohui Yan, Ruimin Li, Guanyu Chen, Xinqi Wang, Yingqiang Wen, Chaohong Zhang, Xiping Wang, Yan Xu, Yuejin Wang\",\"doi\":\"10.1093/hr/uhad116\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Grapevine powdery mildew is caused by <i>Erysiphe necator</i>, which seriously harms grape production in the world. Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew. A novel <i>VqNSTS3</i> was identified and cloned from Chinese wild <i>Vitis quinquangularis</i> accession Danfeng-2. The novel <i>VqNSTS3</i> was transferred into susceptible 'Thompson Seedless' by <i>Agrobacterium</i>-mediated transformation. The transgenic plants showed resistance to the disease and activated other resistance-related genes. <i>VqNSTS3</i> expression in grapevine is regulated by VqWRKY33, and which binds to TTGACC in the <i>VqNSTS3</i> promoter. Furthermore, VqWRKY33 was phosphorylated by VqMAPK3/VqMAPK6 and thus led to enhanced signal transduction and increased <i>VqNSTS3</i> expression. <i>Pro</i><i>VqNSTS3::VqNSTS3</i>-GFP of transgenic <i>VqNSTS3</i> in <i>Arabidopsis thaliana</i> was observed to move to and wrap the pathogen's haustoria and block invasion by <i>Golovinomyces cichoracearum</i>. These results demonstrate that stilbene accumulation of novel <i>VqNSTS3</i> of the Chinese wild <i>Vitis quinquangularis</i> accession Danfeng-2 prevented pathogen invasion and enhanced resistance to powdery mildew. Therefore, <i>VqNSTS3</i> can be used in generating powdery mildew-resistant grapevines.</p>\",\"PeriodicalId\":57479,\"journal\":{\"name\":\"园艺研究(英文)\",\"volume\":\"10 7\",\"pages\":\"uhad116\"},\"PeriodicalIF\":7.6000,\"publicationDate\":\"2023-05-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541564/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"园艺研究(英文)\",\"FirstCategoryId\":\"1091\",\"ListUrlMain\":\"https://doi.org/10.1093/hr/uhad116\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/7/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"园艺研究(英文)","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.1093/hr/uhad116","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
VqMAPK3/VqMAPK6, VqWRKY33, and VqNSTS3 constitute a regulatory node in enhancing resistance to powdery mildew in grapevine.
Grapevine powdery mildew is caused by Erysiphe necator, which seriously harms grape production in the world. Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew. A novel VqNSTS3 was identified and cloned from Chinese wild Vitis quinquangularis accession Danfeng-2. The novel VqNSTS3 was transferred into susceptible 'Thompson Seedless' by Agrobacterium-mediated transformation. The transgenic plants showed resistance to the disease and activated other resistance-related genes. VqNSTS3 expression in grapevine is regulated by VqWRKY33, and which binds to TTGACC in the VqNSTS3 promoter. Furthermore, VqWRKY33 was phosphorylated by VqMAPK3/VqMAPK6 and thus led to enhanced signal transduction and increased VqNSTS3 expression. ProVqNSTS3::VqNSTS3-GFP of transgenic VqNSTS3 in Arabidopsis thaliana was observed to move to and wrap the pathogen's haustoria and block invasion by Golovinomyces cichoracearum. These results demonstrate that stilbene accumulation of novel VqNSTS3 of the Chinese wild Vitis quinquangularis accession Danfeng-2 prevented pathogen invasion and enhanced resistance to powdery mildew. Therefore, VqNSTS3 can be used in generating powdery mildew-resistant grapevines.
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