园艺研究(英文)最新文献_第6页

AlliumDB: a central portal for comparative and functional genomics in Allium. AlliumDB：薤白比较和功能基因组学的中心门户。

园艺研究(英文) Pub Date : 2023-12-29 eCollection Date: 2024-02-01 DOI: 10.1093/hr/uhad285

Pengtao Yang, Yu Yuan, Chao Yan, Yue Jia, Qi You, Lingling Da, Ao Lou, Bingsheng Lv, Zhonghua Zhang, Yue Liu

{"title":"AlliumDB: a central portal for comparative and functional genomics in Allium.","authors":"Pengtao Yang, Yu Yuan, Chao Yan, Yue Jia, Qi You, Lingling Da, Ao Lou, Bingsheng Lv, Zhonghua Zhang, Yue Liu","doi":"10.1093/hr/uhad285","DOIUrl":"10.1093/hr/uhad285","url":null,"abstract":"The genus Allium belongs to the botanical family Amaryllidaceae and includes economically important crops such as onion, garlic, bunching onion, and leek, used as vegetables, spices, and traditional medicines. The large sizes of Allium genomes hamper the genetic dissection of agronomically important traits and molecular breeding. With the growing accumulation of genomic, resequencing, transcriptome, and phenotypic data, the demand for an integrative Allium database is increasing. Here we present a user-friendly database, AlliumDB (https://allium.qau.edu.cn), as a functional genomics hub integrating public and in-house data. The database contains all currently available nuclear and organelle genomes for Allium species, with genes comprehensively annotated based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, orthology, gene families, protein families (Pfam), and non-coding RNA families (Rfam). Transcriptome and variation profiles are integrated into dynamic visualization tools. We took phenotypic photographs and generated trait records for hundreds of Allium germplasms collected worldwide, which are included in the database. We incorporated JBrowse for the visualization of gene structures, RNA sequencing data, and variation data. Analysis tools such as the basic local alignment search tool (BLAST), sequence fetch, enrichment, and motif analyses are available to explore potential gene functions. This database incorporates comprehensive Allium genotypic and phenotypic datasets. As the community assembles new genomes and generates resequencing data for Allium germplasms, the database will be improved and continuously updated with these multi-omics data and comparative genomic studies. We expect the AlliumDB database to become a key resource for the study of Allium crops.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 2","pages":"uhad285"},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10871970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139900979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IAA-miR164a-NAC100L1 module mediates symbiotic incompatibility of cucumber/pumpkin grafted seedlings through regulating callose deposition. IAA-miR164a-NAC100L1模块通过调节胼胝质沉积介导黄瓜/南瓜嫁接苗的共生不相容。

园艺研究(英文) Pub Date : 2023-12-29 eCollection Date: 2024-02-01 DOI: 10.1093/hr/uhad287

Mingzhu Yuan, Tong Jin, Jianqiang Wu, Lan Li, Guangling Chen, Jiaqi Chen, Yu Wang, Jin Sun

{"title":"IAA-miR164a-NAC100L1 module mediates symbiotic incompatibility of cucumber/pumpkin grafted seedlings through regulating callose deposition.","authors":"Mingzhu Yuan, Tong Jin, Jianqiang Wu, Lan Li, Guangling Chen, Jiaqi Chen, Yu Wang, Jin Sun","doi":"10.1093/hr/uhad287","DOIUrl":"10.1093/hr/uhad287","url":null,"abstract":"Grafting is one of the key technologies to overcome the obstacles of continuous cropping, and improve crop yield and quality. However, the symbiotic incompatibility between rootstock and scion affects the normal growth and development of grafted seedlings after survival. The specific molecular regulation mechanism of graft incompatibility is still largely unclear. In this study, we found that the IAA-miR164a-NAC100L1 module induced callose deposition to mediate the symbiotic incompatibility of cucumber/pumpkin grafted seedlings. The incompatible combination (IG) grafting interface accumulated more callose, and the activity of callose synthase (CmCalS1) and IAA content were significantly higher than in the compatible combination (CG). Treatment with IAA polar transport inhibitor in the root of the IG plants decreased CmCalS activity and callose content. Furthermore, IAA negatively regulated the expression of Cm-miR164a, which directly targeted cleavage of CmNAC100L1. Interestingly, CmNAC100L1 interacted with CmCalS1 to regulate its activity. Further analysis showed that the interaction between CmNAC100L1 and CmCalS1 increased the activity of CmCalS1 in the IG plants but decreased it in the CG plants. Point mutation analysis revealed that threonine at the 57th position of CmCalS1 protein played a critical role to maintain its enzyme activity in the incompatible rootstock. Thus, IAA inhibited the expression of Cm-miR164a to elevate the expression of CmNAC100L1, which promoted CmNAC100L1 interaction with CmCalS1 to enhance CmCalS1 activity, resulting in callose deposition and symbiotic incompatibility of cucumber/pumpkin grafted seedlings.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 2","pages":"uhad287"},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10873582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139900980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chromosome-level genome assembly of Agave hybrid NO.11648 provides insights into the CAM photosynthesis. 龙舌兰杂交种 NO.11648 的染色体级基因组组装提供了对 CAM 光合作用的深入了解。

园艺研究(英文) Pub Date : 2023-12-19 eCollection Date: 2024-02-01 DOI: 10.1093/hr/uhad269

Ziping Yang, Qian Yang, Qi Liu, Xiaolong Li, Luli Wang, Yanmei Zhang, Zhi Ke, Zhiwei Lu, Huibang Shen, Junfeng Li, Wenzhao Zhou

{"title":"A chromosome-level genome assembly of Agave hybrid NO.11648 provides insights into the CAM photosynthesis.","authors":"Ziping Yang, Qian Yang, Qi Liu, Xiaolong Li, Luli Wang, Yanmei Zhang, Zhi Ke, Zhiwei Lu, Huibang Shen, Junfeng Li, Wenzhao Zhou","doi":"10.1093/hr/uhad269","DOIUrl":"10.1093/hr/uhad269","url":null,"abstract":"The subfamily Agavoideae comprises crassulacean acid metabolism (CAM), C3, and C4 plants with a young age of speciation and slower mutation accumulation, making it a model crop for studying CAM evolution. However, the genetic mechanism underlying CAM evolution remains unclear because of lacking genomic information. This study assembled the genome of Agave hybrid NO.11648, a constitutive CAM plant belonging to subfamily Agavoideae, at the chromosome level using data generated from high-throughput chromosome conformation capture, Nanopore, and Illumina techniques, resulting in 30 pseudo-chromosomes with a size of 4.87 Gb and scaffold N50 of 186.42 Mb. The genome annotation revealed 58 841 protein-coding genes and 76.91% repetitive sequences, with the dominant repetitive sequences being the I-type repeats (Copia and Gypsy accounting for 18.34% and 13.5% of the genome, respectively). Our findings also provide support for a whole genome duplication event in the lineage leading to A. hybrid, which occurred after its divergence from subfamily Asparagoideae. Moreover, we identified a gene duplication event in the phosphoenolpyruvate carboxylase kinase (PEPCK) gene family and revealed that three PEPCK genes (PEPCK3, PEPCK5, and PEPCK12) were involved in the CAM pathway. More importantly, we identified transcription factors enriched in the circadian rhythm, MAPK signaling, and plant hormone signal pathway that regulate the PEPCK3 expression by analysing the transcriptome and using yeast one-hybrid assays. Our results shed light on CAM evolution and offer an essential resource for the molecular breeding program of Agave spp.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 2","pages":"uhad269"},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10848310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139708696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A biosynthetic network for protoberberine production in Coptis chinensis. 黄连生产原小檗碱的生物合成网络。

IF 7.6

园艺研究(英文) Pub Date : 2023-12-13 eCollection Date: 2024-01-01 DOI: 10.1093/hr/uhad259

Linrui Wu, Binxin Zhao, Zixin Deng, Bin Wang, Yi Yu

{"title":"A biosynthetic network for protoberberine production in Coptis chinensis.","authors":"Linrui Wu, Binxin Zhao, Zixin Deng, Bin Wang, Yi Yu","doi":"10.1093/hr/uhad259","DOIUrl":"10.1093/hr/uhad259","url":null,"abstract":"Protoberberine alkaloids are a group of tetracyclic isoquinoline compounds known for their well-established antimicrobial and anti-inflammatory properties. The richness and diversity of protoberberine alkaloids accumulated in the Coptis genus necessitate a comprehensive examination of the biosynthetic machinery to understand their ecological significance. Here, from Coptis chinensis we identified CcCYP719A1, which could install a methylenedioxy bridge on either ring A or ring D of the protoberberine backbone, thus diverging metabolite flux towards the biosynthesis of various protoberberine components. We also obtained CcCYP719A2 and CcCYP719A3, which underwent positive selection after diverging from CcCYP719A1 and maintained specific catalytic activity on ring D. Further, we resolved the biosynthetic pathway of jatrorrhizine by identifying two demethylases, which could also modulate protoberberine composition by removing the C-3 methyl group and methylenedioxy bridge of ring D, allowing demethylated metabolites to be redirected into different routes. Moreover, we characterized 2-O-methyltransferase CcOMT1 and flavin-dependent oxidase CcTHBO, respectively responsible for the commonly observed 2-O-methylation and aromatic ring-C assembly in protoberberine alkaloids. Overall, this study reveals an interconnected metabolite network from which diverse protoberberine alkaloids originate. It provides valuable insights into the existence of undiscovered protoberberine components, and paves the way for the targeted production of desired protoberberine components for potential therapeutic development.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 1","pages":"uhad259"},"PeriodicalIF":7.6,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10812381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139572188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A highly diversified NLR cluster in melon contains homologs that confer powdery mildew and aphid resistance. 甜瓜中一个高度多样化的 NLR 簇包含赋予白粉病和蚜虫抗性的同源物。

IF 7.6

园艺研究(英文) Pub Date : 2023-12-13 eCollection Date: 2024-01-01 DOI: 10.1093/hr/uhad256

Nathalie Boissot, Veronique Chovelon, Vincent Rittener-Ruff, Nathalie Giovinazzo, Pascale Mistral, Michel Pitrat, Myriam Charpentier, Christelle Troadec, Abdelhafid Bendahmane, Catherine Dogimont

{"title":"A highly diversified NLR cluster in melon contains homologs that confer powdery mildew and aphid resistance.","authors":"Nathalie Boissot, Veronique Chovelon, Vincent Rittener-Ruff, Nathalie Giovinazzo, Pascale Mistral, Michel Pitrat, Myriam Charpentier, Christelle Troadec, Abdelhafid Bendahmane, Catherine Dogimont","doi":"10.1093/hr/uhad256","DOIUrl":"10.1093/hr/uhad256","url":null,"abstract":"Podosphaera xanthii is the main causal agent of powdery mildew (PM) on Cucurbitaceae. In Cucumis melo, the Pm-w resistance gene, which confers resistance to P. xanthii, is located on chromosome 5 in a cluster of nucleotide-binding leucine-rich repeat receptors (NLRs). We used positional cloning and transgenesis, to isolate the Pm-wWMR 29 gene encoding a coiled-coil NLR (CC-NLR). Pm-wWMR 29 conferred high level of resistance to race 1 of PM and intermediate level of resistance to race 3 of PM. Pm-wWMR 29 turned out to be a homolog of the Aphis gossypii resistance gene Vat-1PI 161375. We confirmed that Pm-wWMR 29 did not confer resistance to aphids, while Vat-1PI 161375 did not confer resistance to PM. We showed that both homologs were included in a highly diversified cluster of NLRs, the Vat cluster. Specific Vat-1PI 161375 and Pm-wWMR 29 markers were present in 10% to 13% of 678 accessions representative of wild and cultivated melon types worldwide. Phylogenic reconstruction of 34 protein homologs of Vat-1PI 161375 and Pm-wWMR 29 identified in 24 melon accessions revealed an ancestor with four R65aa-a specific motif in the LRR domain, evolved towards aphid and virus resistance, while an ancestor with five R65aa evolved towards PM resistance. The complexity of the cluster comprising the Vat/Pm-w genes and its diversity in melon suggest that Vat homologs may contribute to the recognition of a broad range of yet to be identified pests and pathogens.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 1","pages":"uhad256"},"PeriodicalIF":7.6,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10807702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139547909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A telomere-to-telomere reference genome of ficus (Ficus hispida) provides new insights into sex determination. 榕树（Ficus hispida）端粒到端粒参考基因组为性别决定提供了新的视角。

IF 7.6

园艺研究(英文) Pub Date : 2023-12-13 eCollection Date: 2024-01-01 DOI: 10.1093/hr/uhad257

Zhenyang Liao, Tianwen Zhang, Wenlong Lei, Yibin Wang, Jiaxin Yu, Yinghao Wang, Kun Chai, Gang Wang, Huahao Zhang, Xingtan Zhang

{"title":"A telomere-to-telomere reference genome of ficus (Ficus hispida) provides new insights into sex determination.","authors":"Zhenyang Liao, Tianwen Zhang, Wenlong Lei, Yibin Wang, Jiaxin Yu, Yinghao Wang, Kun Chai, Gang Wang, Huahao Zhang, Xingtan Zhang","doi":"10.1093/hr/uhad257","DOIUrl":"10.1093/hr/uhad257","url":null,"abstract":"A high-quality reference genome is indispensable for resolving biologically essential traits. Ficus hispida is a dioecious plant. A complete Ficus reference genome will be crucial for understanding their sex evolution and important biological characteristics, such as aerial roots, mutualistic symbiosis with ficus-wasps, and fruiting from old stems. Here, we generated a telomere-to-telomere (T2T) genome for F. hispida using PacBio HiFi and Oxford Nanopore Ultra-long sequencing technologies. The genome contiguity and completeness has shown improvement compared with the previously released genome, with the annotation of six centromeres and 28 telomeres. We have refined our previously reported 2-Mb male-specific region into a 7.2-Mb genomic region containing 51 newly predicted genes and candidate sex-determination genes AG2 and AG3. Many of these genes showed extremely low expression, likely attributed to hypermethylation in the gene body and promoter regions. Gene regulatory networks (GRNs) revealed that AG2 and AG3 are related to the regulation of stamen development in male flowers, while the AG1 gene is responsible for regulating female flowers' defense responses and secondary metabolite processes. Comparative analysis of GRNs showed that the NAC, WRKY, and MYB transcription factor families dominate the female GRN, whereas the MADS and MYB transcription factor families are prevalent in the male GRN.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 1","pages":"uhad257"},"PeriodicalIF":7.6,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10807705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139547912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An efficient protoplast-based genome editing protocol for Vitis species. 基于原生质体的高效葡萄基因组编辑方案。

园艺研究(英文) Pub Date : 2023-12-13 eCollection Date: 2024-01-01 DOI: 10.1093/hr/uhad266

David M Tricoli, Juan M Debernardi

{"title":"An efficient protoplast-based genome editing protocol for Vitis species.","authors":"David M Tricoli, Juan M Debernardi","doi":"10.1093/hr/uhad266","DOIUrl":"10.1093/hr/uhad266","url":null,"abstract":"CRISPR-Cas technologies allow for precise modifications in plant genomes and promise to revolutionize agriculture. These technologies depend on the delivery of editing components into plant cells and the regeneration of fully edited plants. In vegetatively propagated plants, such as grape, protoplast culture provides one of the best avenues for producing non-chimeric and transgene-free genome-edited plants. However, poor regeneration of plants from protoplasts has hindered their implementation for genome editing. Here, we report an efficient protocol for regenerating plants from protoplasts from multiple grape varieties. By encapsulating the protoplasts in calcium alginate beads and co-culturing them with feeder cultures, the protoplasts divide to form callus colonies that regenerate into embryos and ultimately plants. This protocol worked successfully in wine and table grape (Vitis vinifera) varieties, as well as grape rootstocks and the grapevine wild relative Vitis arizonica. Moreover, by transfecting protoplasts with CRISPR-plasmid or ribonucleoprotein (RNP) complexes, we regenerated albino plants with edits in VvPHYTOENE DESATURASE gene in three varieties and in V. arizonica. The results reveal the potential of this platform to facilitate genome editing in Vitis species.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 1","pages":"uhad266"},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11184525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Ca²⁺ sensor BraCBL1.2 involves in BraCRa-mediated clubroot resistance in Chinese cabbage. Ca2+传感器BraCBL1.2参与了BraCRa介导的大白菜抗球根病的过程。

IF 7.6

园艺研究(英文) Pub Date : 2023-12-13 eCollection Date: 2024-01-01 DOI: 10.1093/hr/uhad261

Yinglan Piao, Shizhen Li, Yiduo Chen, Sisi Zhao, Zhongyun Piao, Haiping Wang

{"title":"A Ca2+ sensor BraCBL1.2 involves in BraCRa-mediated clubroot resistance in Chinese cabbage.","authors":"Yinglan Piao, Shizhen Li, Yiduo Chen, Sisi Zhao, Zhongyun Piao, Haiping Wang","doi":"10.1093/hr/uhad261","DOIUrl":"10.1093/hr/uhad261","url":null,"abstract":"Clubroot disease caused by Plasmodiophora brassicae (P. brassicae) severely threatens the cultivation of Cruciferous plants, especially Chinese cabbage. Recently, resistance genes in plants have been reported to encode for a Ca2+-permeable channel in the plasma membrane, which can mediate the cytosolic Ca2+ increase in plant cells upon pathogen attack. However, the downstream Ca2+ sensor and decoder are still unknown. In this study, we identified the virulent and avirulent P. brassicae isolates (Pbs) of two near isogenic lines, CR 3-2 and CS 3-2, with CR 3-2 harboring clubroot resistant gene BraCRa. The transcriptomic analysis was then conducted with CR 3-2 after inoculating with virulent isolate PbE and avirulent isolate Pb4. From the differentially expressed genes of transcriptomic data, we identified a Ca2+-sensor encoding gene, BraCBL1.2, that was highly induced in CR 3-2 during infection by Pb4 but not by PbE. Moreover, GUS histochemical staining and subcellular localization analysis revealed that BraCBL1.2 was specifically expressed in the root hair cells of Arabidopsis and encoded a putative Ca2+ sensor localized in the plasma membrane. We also developed an assay to investigate the BraCRa-mediated hypersensitive response (HR) in tobacco leaves. The results suggest that BraCBL1.2 is involved in the BraCRa-mediated plant ETI immune response against P. brassicae. In addition, we verified that overexpression of BraCBL1.2 enhanced clubroot resistance in Arabidopsis. Collectively, our data identified the involvement of a Ca2+ sensor in BraCRa-mediated clubroot resistance in Chinese cabbage, providing a theoretical basis for further research on the resistance of Chinese cabbage to P. brassicae.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 1","pages":"uhad261"},"PeriodicalIF":7.6,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10828780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139652291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signalling mechanisms and agricultural applications of (Z)-3-hexenyl butyrate-mediated stomatal closure. (Z)-3-己烯基丁酸酯介导的气孔关闭的信号机制和农业应用。

园艺研究(英文) Pub Date : 2023-11-28 eCollection Date: 2024-01-01 DOI: 10.1093/hr/uhad248

Celia Payá, Borja Belda-Palazón, Francisco Vera-Sirera, Julia Pérez-Pérez, Lucía Jordá, Ismael Rodrigo, José María Bellés, María Pilar López-Gresa, Purificación Lisón

{"title":"Signalling mechanisms and agricultural applications of (Z)-3-hexenyl butyrate-mediated stomatal closure.","authors":"Celia Payá, Borja Belda-Palazón, Francisco Vera-Sirera, Julia Pérez-Pérez, Lucía Jordá, Ismael Rodrigo, José María Bellés, María Pilar López-Gresa, Purificación Lisón","doi":"10.1093/hr/uhad248","DOIUrl":"10.1093/hr/uhad248","url":null,"abstract":"Biotic and abiotic stresses can severely limit crop productivity. In response to drought, plants close stomata to prevent water loss. Furthermore, stomata are the main entry point for several pathogens. Therefore, the development of natural products to control stomata closure can be considered a sustainable strategy to cope with stresses in agriculture. Plants respond to different stresses by releasing volatile organic compounds. Green leaf volatiles, which are commonly produced across different plant species after tissue damage, comprise an important group within volatile organic compounds. Among them, (Z)-3-hexenyl butyrate (HB) was described as a natural inducer of stomatal closure, playing an important role in stomatal immunity, although its mechanism of action is still unknown. Through different genetic, pharmacological, and biochemical approaches, we here uncover that HB perception initiates various defence signalling events, such as activation of Ca2+ permeable channels, mitogen-activated protein kinases, and production of Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-mediated reactive oxygen species. Furthermore, HB-mediated stomata closure was found to be independent of abscisic acid biosynthesis and signalling. Additionally, exogenous treatments with HB alleviate water stress and improve fruit productivity in tomato plants. The efficacy of HB was also tested under open field conditions, leading to enhanced resistance against Phytophthora spp. and Pseudomonas syringae infection in potato and tomato plants, respectively. Taken together, our results provide insights into the HB signalling transduction pathway, confirming its role in stomatal closure and plant immune system activation, and propose HB as a new phytoprotectant for the sustainable control of biotic and abiotic stresses in agriculture.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 1","pages":"uhad248"},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10794947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139493037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis. 构建长豆角的高密度遗传图谱并鉴定 ANT1 作为花青素生物合成的调节因子。

IF 7.6

园艺研究(英文) Pub Date : 2023-11-27 eCollection Date: 2024-01-01 DOI: 10.1093/hr/uhad247

Hongmei Zhang, Wei Zhang, Shan Meng, Linchong Hui, Xiaoqing Liu, Wei Chen, Wei Yan, Xin Chen, Huatao Chen

{"title":"Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis.","authors":"Hongmei Zhang, Wei Zhang, Shan Meng, Linchong Hui, Xiaoqing Liu, Wei Chen, Wei Yan, Xin Chen, Huatao Chen","doi":"10.1093/hr/uhad247","DOIUrl":"10.1093/hr/uhad247","url":null,"abstract":"Because its long, tender pods supply essential proteins, vitamins, and fibers to humans, yardlong bean (Vigna unguiculata ssp. sesquipedalis) is a commonly consumed vegetable, especially in Southeast Asia. To provide insights into the genetic bases of key agricultural traits in yardlong bean, we here created a high-density bin-map with 2084 bin markers using 514 227 SNPs from a recombinant-inbred line (RIL) population. Quantitative trait loci (QTL) mapping was carried out to identify loci associated with anthocyanin content (ANT), vitamin E content (VE), total soluble protein content (TSP), pod length (PL), hundred-seed weight (HSW), seed length and width (SL and SW, respectively), and seed coat color (SCC). In total, 20 related QTLs were isolated, explaining 7.58-56.03% of the phenotypic variation. Of these, five major QTLs (qANT5, qTSP11, qVE7, qPL3, and qSCC9) were detected in 2020, 2021, and the combined environment, explaining 11.96-56.03% of the phenotypic variation. VuANT1 was identified as a causal gene for the QTL qANT5, which regulated anthocyanin content; VuANT1 was highly expressed in immature purple pods but barely detectable in white pods. VuANT1 overexpression in tobacco leaves and yardlong bean hairy roots resulted in purple coloration as a result of anthocyanin accumulation. These findings suggested that VuANT1 was a key regulator of anthocyanin accumulation in yardlong bean. Our results lay a firm foundation for target agricultural trait improvement and clarification of the genetic mechanisms underlying agricultural traits in yardlong bean.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 1","pages":"uhad247"},"PeriodicalIF":7.6,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10809905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139565334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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