园艺研究(英文)最新文献

{"title":"The transcription factor <i>BpERF1A</i> enhances drought tolerance in <i>Betula platyphylla</i> by activating dehydrin and ROS scavenging through interaction with BpRAV1.","authors":"Meiqi Zhou, Yuan Zhang, Zhen Miao, Weiyi Bi, Xu Li, Yang Li, Chao Wang","doi":"10.1093/hr/uhag133","DOIUrl":"10.1093/hr/uhag133","url":null,"abstract":"<p><p>Although ERF transcription factors (TFs) play critical roles in abiotic stress tolerance, the molecular mechanisms underlying this role remain incompletely understood. This study identified <i>BpERF1A</i> in birch (<i>Betula platyphylla</i>) as a drought-responsive TF through co-expression regulatory network analysis. Expression of <i>BpERF1A</i> was stalwartly induced by drought stress, and drought treatment markedly boosted its promoter activity. Functional analyses demonstrated that overexpression of <i>BpERF1A</i> markedly improved drought tolerance compared with wild-type (WT) birch, whereas its repression increased drought sensitivity. Overexpression lines also exhibited higher antioxidant enzyme activities and proline content relative to WT. Chromatin immunoprecipitation-polymerase chain reaction, yeast one-hybrid and dual-luciferase (dual-LUC) assays confirmed that <i>BpERF1A</i> directly binds to G-box elements in the promoters of <i>BpDHN</i> (dehydrin) and <i>BpAOS</i> (allene oxide synthase), activating their transcription. Furthermore, overexpression of <i>BpDHN/BpAOS</i> enhanced drought tolerance and promoted reactive oxygen species (ROS) scavenging in transgenic plants. Protein-protein interaction analysis using bimolecular fluorescence complementation revealed that BpERF1A interacts with BpRAV1 to form a heterodimer, which further enhances BpERF1A binding to the <i>BpDHN</i> promoter and its transcriptional activation. Collectively, these findings establish a central role for the <i>BpERF1A</i> regulatory module in drought acclimation and highlight its synergistic interaction with BpRAV1, providing an effective strategy to enhance drought tolerance through improved ROS scavenging capacity.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag133"},"PeriodicalIF":9.5,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13437086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148674633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting cytosine base editing in potato through synergistic optimization.","authors":"Yan Zhang, Lumin Zhang, Jiuzhou Deng, Huiying Zhou, Enle Xiao, Guangtao Zhu, Chunzhi Zhang, Kaiyuan Chen","doi":"10.1093/hr/uhag122","DOIUrl":"10.1093/hr/uhag122","url":null,"abstract":"<p><p>Base editing enables precise substitution of single nucleotides and is a promising technology for improvement of agronomic traits, including those of potato (<i>Solanum tuberosum</i>), the third most important food crop globally. Here, we developed efficient cytosine base editors (CBEs) for potato via multidimensional optimization. By evaluating several highly active cytidine deaminases, we identified two deaminases with high efficiency and distinct characteristics, which were used to construct CBEs for divergent editing applications. Furthermore, the editing efficiency of CBEs was significantly enhanced through several strategies: employing the <i>Arabidopsis thaliana RPS5A</i> promoter and the tobacco mosaic virus Ω enhancer to boost the expression of the editing reagents, fusing chromatin-modulating peptides, and co-expressing the human RNA m⁶A demethylase gene <i>hFTO</i> to enhance chromatin accessibility. In a hairy root assay, an efficient optimized base editor, RF-Sdd7-HNHN, elevated the average editing efficiency nearly 3-fold, from 26.8% (pre-optimization) to 76.7%. Using RF-Sdd7-HNHN, we achieved an 85.7% efficiency for simultaneous editing of target nucleotides in <i>ACETOLACTATE SYNTHASE 1</i> (<i>StALS1</i>) and <i>StALS2</i> and obtained homozygous mutant potato germplasm exhibiting herbicide resistance. In conclusion, this research established new and efficient CBEs for potato and offers insights and strategies for optimizing other CRISPR-based editing tools.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag122"},"PeriodicalIF":9.5,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13395515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148581344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"REF1 peptides significantly enhance transformation efficiency but inhibit shoot regeneration in citrus <i>agrobacterium</i>-mediated transformation.","authors":"Yu Feng, Wenting Wang, Yuanchun Wang, Xiaoen Huang, Jin Xu, Nian Wang","doi":"10.1093/hr/uhag134","DOIUrl":"10.1093/hr/uhag134","url":null,"abstract":"<p><p><i>Agrobacterium</i>-mediated transformation remains challenging for economically important citrus crops because of low transformation and regeneration efficiencies. REGENERATION FACTOR1 (REF1), a plant elicitor peptide, is a local wound signal perceived by PROPEP RECEPTOR-LIKE KINASE 1 (PORK1) and transduced via WOUND-INDUCED DEDIFFERENTIATION 1 (WIND1). Exogenous REF1 peptide application has recently been reported to improve transformation and regeneration of herbaceous plants including tomato, which was explored on <i>Agrobacterium</i>-mediated citrus transformation in this study. We identified REF1, PORK1, and WIND1 orthologs in sweet orange (<i>Citrus sinensis</i>), which encodes two REF1 isoforms, CsREF1-1 and CsREF1-2. REF1 of tomato (SlREF1) and the two CsREF1 isoforms of different concentrations were tested for their effect on <i>Agrobacterium</i>-mediated transformation of citrus via exogenous application. CsREF1-1 at 10 or 100 nM and CsREF1-2 at 100 nM significantly increased GFP-positive transgenic callus formation, whereas SlREF1 had no significant effect. In addition, CsREF1-1, CsREF1-2, and SlREF1 reduced shoot regeneration. To overcome this trade-off, we exposed epicotyl explants to 100 nM CsREF1-2 only during the first month and then transferred them to REF1-free regeneration medium, yielding 4.1-fold more GFP-positive shoots than the untreated control. Such a staged application of CsREF1-2 also improved GFP-positive shoot recovery in Carrizo citrange by 3.3-fold. Reverse transcription-quantitative PCR (RT-qPCR) showed that CsREF1-2 induced <i>CsWIND1</i> and <i>CsESR1,</i> which are critical for callus formation and shoot regeneration, but continuous CsREF1-2 exposure also suppressed shoot/meristem regulators, which was mitigated by removal of CsREF1-2 in the later stage. AlphaFold3 and PRODIGY predictions indicated the highest binding affinity for CsREF1-2 with CsPORK1, consistent with its superior activity. Overall, both CsREF1 peptides enhance citrus transformation but suppress regeneration, and staged exogenous application of CsREF1 peptides improves genetic improvement of recalcitrant perennial crops such as citrus, which decouples early dedifferentiation from later organogenesis, substantially expanding the toolbox for high-throughput functional genomics in citrus.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag134"},"PeriodicalIF":9.5,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148681157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic basis of scent loss and functional divergence in wild and cultivated carnations (<i>Dianthus</i> spp.).","authors":"Jesús Picazo-Aragonés, Stefan Dötterl, Ovidiu Paun, Anass Terrab, Francisco Balao","doi":"10.1093/hr/uhag130","DOIUrl":"10.1093/hr/uhag130","url":null,"abstract":"<p><p>Domestication reshapes plant genomes and traits, yet the genomic basis of floral scent loss remains unclear. Here, we present an 876 Mb pseudo-chromosome genome assembly of the wild carnation <i>Dianthus broteri</i> (2<i>n</i> = 2<i>x</i> = 30) and compare it with the cultivated <i>D. caryophyllus</i>, which exhibits reduced floral scent. Transposable elements occupy 82.4% of the <i>D. broteri</i> genome, dominated by Gypsy LTRs, whereas <i>D. caryophyllus</i> exhibits more recent insertions near genes, consistent with domestication associated effects. Volatilome profiling of <i>D. broteri</i> revealed 59 floral volatiles, dominated by sesquiterpenoids, with lineage-specific differences in composition. Terpenoid-biosynthesis genes are upregulated in flowers of the scented western lineage, while the weakly scented eastern lineage shows reduced expression of upstream pathway components despite constitutive transcription of some terpene synthases. Variant discovery within terpenoid pathway genes showed that the most highly and constitutively expressed gene, <i>DbrTPS18,</i> accounted for almost 50% of all lineage-specific variants. Several nonsynonymous substitutions in <i>DbrTPS18</i> were identified within the catalytic domains and the predicted chloroplast transit peptide, and these variants were significantly associated with variation in scent production. These results uncover the basis of terpenoid biosynthesis in the genus <i>Dianthus</i> and, together with the presented reference genome, provides a foundation to explore the genomic consequences of domestication and enhance fragrance in cultivated carnations.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag130"},"PeriodicalIF":9.5,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethylene-responsive CmERF3-CmMYB4 cascade suppresses anthocyanin biosynthesis in <i>Chrysanthemum</i> ×<i>morifolium</i>.","authors":"MengLing Li, WenJing Zhao, ShuangDa Li, XiaoHui Wen, Xiang Song, BoHao Wang, YuanYuan Wei, BoXiao Fu, LuYao Wang, YuanQi Dou, PengRui Jiang, He Huang, SiLan Dai, Yan Hong","doi":"10.1093/hr/uhag132","DOIUrl":"10.1093/hr/uhag132","url":null,"abstract":"<p><p>Anthocyanins are the primary determinants of floral pigmentation in <i>Chrysanthemum</i> ×<i>morifolium</i>, and ethylene acts as a key regulator of their biosynthesis. Although the ethylene-mediated regulatory circuitry is functionally important, its underlying mechanism in chrysanthemum has remained unclear. In this study, we identified CmMYB4 as a transcriptional repressor that directly suppresses the expression of key anthocyanin biosynthetic genes, including <i>CmDFR</i> (dihydroflavonol 4-reductase), <i>CmUFGT</i> (flavonoid 3-<i>O</i>-glucosyltransferase), and <i>Cm3MaT</i> (anthocyanin 3-<i>O</i>-glucoside-6″-<i>O</i>-malonyltransferase). Time-ordered gene co-expression network analysis comparing the transcriptomes of <i>CmMYB4</i>-overexpressing and control plants, together with molecular biology experimental results, further revealed <i>CmERF3</i> (<i>ethylene response factor 3</i>) as a hierarchical upstream regulator of anthocyanin biosynthesis. Exogenous ethylene treatment induced <i>CmERF3</i> expression while reducing anthocyanin accumulation. Subsequent functional characterization showed that the overexpression of <i>CmERF3</i> suppresses anthocyanin biosynthesis in both tobacco and chrysanthemum by directly activating <i>CmMYB4</i> and repressing <i>CmDFR</i>, <i>CmUFGT</i>, and <i>Cm3MaT</i>. Collectively, these findings revealed that ethylene inhibits anthocyanin biosynthesis through a CmERF3-CmMYB4-LBGs (late biosynthetic genes) regulatory module. This study not only elucidates the molecular mechanism governing ethylene-mediated anthocyanin inhibition but also provides new perspectives for the molecular engineering of ornamental traits in chrysanthemum.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag132"},"PeriodicalIF":9.5,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13437082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148674578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A transposable element-derived regulatory variation in <i>RsOFP2.3</i> underlies morphological diversification of radish taproots through the conserved OVATE family protein-TONNEAU1-recruiting motif module.","authors":"Yanping Wang, Xiangyu Wu, Yuanting Pang, Dingzhu Hua, Ting Chi, Ailing Ge, Tongbing Su, Qingbiao Wang, Li Zhang","doi":"10.1093/hr/uhag127","DOIUrl":"10.1093/hr/uhag127","url":null,"abstract":"<p><p>Radish (<i>Raphanus sativus</i> L.) represents a major root vegetable crop exhibiting remarkable variation in taproot morphology, yet the underlying genetic and molecular mechanisms remain poorly understood. Through a structural variant (SV)-based genome-wide association study (GWAS), we identified <i>RsOFP2.3</i>, encoding an OVATE Family Protein (OFP), as a major determinant of fleshy taproot shape. Comprehensive expression profiling and RNA <i>in situ</i> hybridization revealed that <i>RsOFP2.3</i> is broadly expressed, with the highest transcript accumulation in cambial tissues. Notably, <i>RsOFP2.3</i> expression was markedly higher in the round-rooted accession than in the long-rooted one. Population-wide analysis showed that <i>RsOFP2.3</i> expression was negatively correlated with taproot length and shape index, but positively correlated with taproot width. A 312-bp transposable element (TE) insertion in the <i>RsOFP2.3</i> promoter repressed its expression and was strongly associated with taproot shape variation, revealing a TE-mediated cis-regulatory mechanism underlying morphological divergence. Functional analyses showed that <i>RsOFP2.3</i> overexpression (OE) promotes radial expansion by enhancing cambial cell division and xylem differentiation, resulting in thicker and shorter taproots, whereas silencing <i>RsOFP2.3</i> produced opposite phenotypic effects. Mechanistically, RsOFP2.3 physically interacts with the TONNEAU1-recruiting motif (TRM) protein RsTRM4, recruiting it from microtubules to the cytoplasm. Downregulation of <i>RsTRM4</i> reduced taproot length, while <i>RsTRM4</i> OE partially alleviated the shortened organ phenotypes caused by <i>RsOFP2.3</i> OE, indicating an antagonistic relationship that fine-tunes organ morphology. These findings uncover two coordinated regulatory mechanisms involving TE-mediated cis-regulatory variation and a conserved OFP-TRM interaction module that jointly shape the balance between radial and longitudinal growth during radish taproot development, providing valuable molecular targets for precision breeding of storage root crops.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag127"},"PeriodicalIF":9.5,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13411279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-nucleus RNA-seq and ATAC-seq analyses provide molecular insights into cadmium-stress response in alfalfa roots.","authors":"Yuqi Zhang, Hao Liu, Ming Xu, Mengjia Xie, Shuhan Deng, Xinyue Ma, Li Zhao, Fei He, Mingna Li, Ruicai Long, Xue Wang, Junmei Kang, Qingchuan Yang, Lin Chen","doi":"10.1093/hr/uhag117","DOIUrl":"10.1093/hr/uhag117","url":null,"abstract":"<p><p>Soil cadmium (Cd) pollution threatens global food security. Elucidating plant cellular responses to Cd stress is critical for the breeding of low-Cd-accumulating crops. Here, we investigated Cd-responsive regulatory mechanisms in alfalfa at single-cell resolution and identified key Cd-associated genes. Eight major root cell types were annotated, with significant remodeling under Cd stress. Under Cd stress, root endodermal and phloem cells adopt distinct adaptive strategies: endodermal cells shift toward a Cd sequestration and detoxification state, whereas phloem cells exhibit a response gradient ranging from basic defense to systemic regulation. Integrated multiomics analyses revealed cell-type-specific genome-wide changes in chromatin accessibility, which positively correlated with gene expression-particularly in promoter regions. Key genes including <i>MsGSH1</i>, <i>MsMT2A</i>, <i>MsHMP47</i>, and <i>MsABCC3</i> were shown to increase Cd tolerance in yeast. Coexpression network analysis revealed 10 cell-type-specific modules, with the calmodulin-like gene <i>MsCML</i> acting as a highly interconnected hub gene, whose overexpression significantly improved Cd tolerance. These findings provide valuable genetic resources and a theoretical basis for the precise breeding of low-Cd-accumulating forage, with implications for understanding Cd-responsive epigenetic and transcriptional regulation in plants.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag117"},"PeriodicalIF":9.5,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13392044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148581374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the <i>MtLUX</i>-<i>MtRVE1</i> regulatory module in auxin-mediated root development and nodule formation in <i>Medicago truncatula</i>.","authors":"Tao Fan, Li-Ping Wang, Jing Li, Ming-Kang Yang, Yue Chen, Meng-Jiao Jin, Liang Chen, Liang-Fa Ge, Wei Huang","doi":"10.1093/hr/uhag124","DOIUrl":"10.1093/hr/uhag124","url":null,"abstract":"<p><p>The circadian clock synchronizes a multitude of biological events with environmental changes, thereby optimizing plant growth and development. In legumes, nodule formation, a pivotal process that sustains symbiotic nitrogen fixation, is one such event regulated by the circadian clock. Nevertheless, the mechanisms underlying the circadian clock's regulation of nodule formation and nitrogen fixation are still poorly elucidated. Herein, we unveil that the core clock gene <i>LUX ARRHYTHMO</i> (<i>LUX</i>) exerts a crucial role in modulating nodule formation and root development via auxin biosynthesis pathways in the model legume <i>Medicago truncatula</i>. Our findings indicate that MtLUX directly associates with the promoter of <i>MtRVE1</i>, a clock output gene involved in auxin biosynthesis, both <i>in vivo</i> and <i>in vitro</i>, thereby repressing its expression. Biochemical and genetic data further corroborate that the <i>MtLUX-MtRVE1</i> regulatory module adjusts root architecture and nodule formation through the fine-tuning of auxin biosynthesis. These discoveries reveal a mechanism whereby the circadian clock integrates hormonal pathways to regulate nodule formation, thereby linking circadian regulation, auxin biosynthesis, and nitrogen fixation in legumes. This research lays the groundwork for enhancing legume growth and nitrogen acquisition under fluctuating environmental conditions.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag124"},"PeriodicalIF":9.5,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13401420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148594608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transcription factor AebHLH89 activates <i>AeGMP1</i> transcription to regulate L-ascorbic acid accumulation in kiwifruit (<i>Actinidia eriantha</i>) revealed by genome-wide association study.","authors":"Lu Chen, Dongfeng Jia, Yansong Liu, Huan Gao, Guanglian Liao, Jipeng Mao, Zhu Gao, Xiaobiao Xu","doi":"10.1093/hr/uhag116","DOIUrl":"10.1093/hr/uhag116","url":null,"abstract":"<p><p><i>Actinidia eriantha</i>, a kiwifruit species endemic to China, produces fruits with notable nutritional, medicinal, and economic value, particularly due to its high L-ascorbic acid (L-AsA) content. However, the regulatory mechanisms underlying L-AsA accumulation in its fruit remain poorly understood. This study meticulously measured L-AsA levels of fruits in 216 <i>A. eriantha</i> accessions from natural populations and performed a genome-wide association study, through which we identified significantly associated lead single nucleotide polymorphisms and insertion deletions, and characterized a key candidate gene <i>AebHLH89</i>, <i>AePPR</i>, <i>AePP2Ab</i>, and <i>AePHL1</i> involved in the positive regulation of L-AsA accumulation. Functional experiments showed that overexpression of <i>AebHLH89</i> significantly enhanced L-AsA accumulation, while its silencing <i>via</i> virus-induced gene silencing markedly decreased L-AsA levels. Yeast one-hybrid assay and dual-luciferase assay preliminary revealed that AebHLH89 could bind to the <i>AeGMP1</i> promoter and activates its transcription, thereby upregulating the L-AsA biosynthesis pathway and promoting L-AsA synthesis and accumulation. These findings provide valuable genetic resources for molecular marker-assisted breeding in kiwifruit and contribute to germplasm innovation. Simultaneously, the identification of key regulatory genes enhances our understanding of L-AsA metabolism and lays a theoretical foundation for the genetic improvement of kiwifruit.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 7","pages":"uhag116"},"PeriodicalIF":9.5,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13341127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148407036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome and machine learning analyses reveal 500-bp promoter chromatin accessibility as a key regulator of gene expression and catechin biosynthesis in <i>Camellia sinensis</i>.","authors":"Yuan Gao, Wenmin Fan, Wenlong Lei, Yibing Chen, Xueli Liao, Yezi Xiao, Lanying Li, Xiaomei Lei, Wanjun Gao, Yingao Zhang, Hongyan Deng, Jiawei Yan, Zongjie Wu, Chen Liang, Xinru Hou, Youben Yu, Fan Luo, Pengjie Wang, Dongna Liu","doi":"10.1093/hr/uhag129","DOIUrl":"10.1093/hr/uhag129","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag129"},"PeriodicalIF":9.5,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13417467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}