园艺研究(英文)最新文献

{"title":"Loss of the nuclear factor Y subunit B3 gene causes chlorosis and chloroplast development defects in cucumber.","authors":"Min Sheng, Siqi Wang, Tiefeng Song, Juyong Zhao, Cancan Xia, Huanle He, Junsong Pan, Jian Pan, Haifan Wen","doi":"10.1093/hr/uhag138","DOIUrl":"10.1093/hr/uhag138","url":null,"abstract":"<p><p>Chlorophyll, the pigment in plant leaves, is crucial for capturing light during photosynthesis. Mutations that disrupt chlorophyll production or chloroplast development frequently lead to changes in leaf color. Here, we report the identification and characterization of a chlorotic mutant in cucumber (<i>Cucumis sativus</i> L.), named <i>tnyl3</i>, which exhibits chlorotic cotyledons and seedling lethality. Map-based cloning revealed that the <i>tnyl3</i> mutation results from a Tnt1 retrotransposon insertion in a gene encoding <i>nuclear factor Y subunit B3</i> (<i>NF-YB3</i>), a transcription factor subunit. Compared with the wild type, the mutant exhibited dramatic decreases in chlorophyll a and b levels and a significantly lower net photosynthetic rate. Ultrastructural analysis revealed that the chloroplasts in the <i>tnyl3</i> mutants are structurally abnormal and characterized by underdeveloped thylakoid membranes. Gene expression analyses revealed that <i>CsNF-YB3</i> is highly expressed in young seedling tissues and is upregulated by light. CRISPR/Cas9 analyses subsequently confirmed that the <i>tnyl3</i> phenotype is caused by the loss of <i>CsNF-YB3</i>. Yeast two-hybrid and split-LUC assays demonstrated that CsNF-YB3 interacts directly with the cucumber NF-YC2 protein and promotes <i>CsTIC21</i> transcription, suggesting that it functions as part of an NF-Y transcriptional complex. Transcriptome profiling of the mutant revealed extensive downregulation of photosynthesis-related genes, which is consistent with its impaired chloroplast function. Our findings establish CsNF-YB3 as a crucial genetic factor for chloroplast development and pigment synthesis in cucumber. This work provides new insight into the NF-Y-mediated regulatory network controlling chloroplast biogenesis and offers a potential genetic target for improving plant photosynthetic performance and vigor.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag138"},"PeriodicalIF":9.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148686588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated omics identifies VviGTs and VviMYB174 as drivers of glycosylated monoterpenoid accumulation in 'Ruiduhongyu' grapes.","authors":"Si-Yu Liu, Xiao-Miao Zhou, Song-Yu Liu, Xiao Meng, Ya-Chen Wang, Lei Sun, Chang-Qing Duan, Bao-Qing Zhu, Qiu-Hong Pan","doi":"10.1093/hr/uhag188","DOIUrl":"10.1093/hr/uhag188","url":null,"abstract":"<p><p>Monoterpenoids contribute to grape and wine varietal aroma as free volatiles and glycosidically bound precursors. While glycosylated forms largely determine postharvest aroma potential, the genes controlling their accumulation remain largely unidentified. Here, we compared free and glycosylated monoterpenoids and the accompanying transcriptome between the green-skinned 'Ruiduxiangyu' (RDXY) and the pink-skinned 'Ruiduhongyu' (RDHY), two siblings derived from the same cross. RDHY berries accumulated higher levels of glycosylated monoterpenoids during ripening, whereas RDXY berries were enriched in the free forms. Integrated metabolome-transcriptome analysis revealed a tight correlation between glycosylated monoterpenoid content and the expression of five glycosyltransferase (<i>GT</i>) genes (<i>VviGTs</i>) and the transcription factor <i>VviMYB174</i>. Besides the previously characterized VviGT14 and VviGT7, enzyme activity assay and transient overexpression experiments in tobacco leaves, grape leaves, and grape berries demonstrated that VviGT22, VviGT37, and VviGT74 also possess monoterpenol glycosylation activity. Yeast one-hybrid assays, combined with overexpression and silencing analyses, confirmed that VviMYB174 directly upregulates these three <i>GT</i> genes in RDHY, thereby promoting glycosylated monoterpenoid accumulation. Sequence alignment revealed that only <i>VviGT74</i> exhibited different promoter sequence divergence between the two cultivars, notably, the <i>VviGT74</i> promoter from RDXY failed to bind <i>VviMYB174</i>, which correlated with its reduced expression in RDXY berries. These findings provide new targets for manipulating the glycosylated aroma precursor pool and advance the molecular understanding of monoterpenoid metabolism in grapevine.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 10","pages":"uhag188"},"PeriodicalIF":9.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in genomics-driven genetic decoding and genomic design breeding in tomato.","authors":"Zihui Ding, Zhangjun Fei, Sanwen Huang, Yaoyao Wu","doi":"10.1093/hr/uhag172","DOIUrl":"10.1093/hr/uhag172","url":null,"abstract":"<p><p>Tomatoes are highly nutritious and represent one of the important vegetable fruits worldwide. Both historically and moving forward, genetic decoding and precision breeding remain fundamental to tomato improvement. Here, we summarize pivotal advances in decoding tomato genomes across domestication, improvement and evolution processes and provide a perspective on future breeding through precision design. In-depth population genetic studies have revealed how artificial selection systematically prioritized yield-related alleles at the cost of narrowing genetic diversity, especially at flavor-related loci-highlighting the urgent need to reconcile these trade-offs. Comparative genomics across species, viewed through an evolutionary lens, has uncovered critical insights into functional genes, deepening our understanding of the genetic architecture and regulatory mechanisms underlying key traits. Collectively, these advances have enabled precise identification and functional characterization of key genetic elements, paving the way for systematic redomestication of tomato through precision genomic design. Looking ahead, more efficient and precise breeding strategies will be required to accelerate genetic gains in tomato in the coming decades. The integration of recent genomic advances, coupled with genomic selection and artificial intelligence, into genomic design breeding offers a transformative framework, unlocking unprecedented opportunities for developing highly flavorful and consumer-customized tomato varieties.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 9","pages":"uhag172"},"PeriodicalIF":9.5,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148882854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of Ca²⁺-ATPases across plants with profiles in <i>Rhododendron</i> and the function of key members in alleviating high calcium stress.","authors":"Sulin Wen, Kui Zhou, Yi Min, Kun Yang, Chunqiong Shang, Lei Peng, Xiaowei Cai, Yuxin Leng, Luonan Shen, Lin Deng, Qingwei Zeng, Hong Zhao, Yunxing Bai, Bingxue Zhang, Manying Zhang, Qian Wang, Fuhua Fan, Xiaopeng Wen, Guang Qiao, Guijie Ding","doi":"10.1093/hr/uhag174","DOIUrl":"10.1093/hr/uhag174","url":null,"abstract":"<p><p>Ca²⁺-ATPase (CAP) is a key Ca²⁺ efflux protein in plants. Our previous research suggests that CAPs may play a crucial role in the adaptation of rhododendrons to high calcium environments. However, the evolution, variation, characteristic expression, and subfunctionalization of this gene family in <i>Rhododendron</i> remain unknown. Through the analysis of pan-genomes and pan-transcriptomes, we elucidated the systematic evolution of CAPs in plants, as well as their characteristic expression patterns in <i>Rhododendron</i>. During the evolutionary process from lower to higher plants, CAPs can be divided into six clades and exhibit structural conservation. CAPs have emerged and differentiated in lower plants such as algae, and they have undergone significant amplification in Eudicots plants like rhododendrons. Three <i>Rhododendron</i> species (<i>Rhododendron bailiense</i>, <i>R. delavayi</i>, and <i>R. irroratum</i>) located in the karst province of Guizhou in Southwest China exhibit the highest copies of CAPs, suggesting a strong association between CAP copy number variation and habitat, particularly in high calcium environments. Through multiple transcriptome analyses, we revealed that CAPs are induced under various environmental/developmental conditions (e.g. karst environments, high altitude, early flower development, hormones, etc.). Co-expression network analysis highlighted key members of calcineurin B-like protein (CBL) and CBL-interacting protein kinases (CIPK) that are associated with the high expression of CAPs. Experimental validation demonstrated that CAPb1 and CAPd1 significantly alleviate high calcium stress, and the CAPb1-CIPK1-CBL1 and CAPd1-CIPK2-CBL1 modules can further enhance the alleviation. These findings provide new insights into the evolution, characteristic expression, and function of CAPs, as well as new perspectives on the high calcium adaptability of rhododendrons.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 9","pages":"uhag174"},"PeriodicalIF":9.5,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13554806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148921918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WalnutGDB: an integrated genomic portal for advancing Juglandaceae research and breeding.","authors":"Honghua Zhang, Langqin Luo, Songyang Li, Zhenyang Shua, Wen Yao, Xiang Luo, Haifang Hu, Xin Chen, Shaowen Quan, Yongqiang Chen, Zhongzhong Guo, Rui Zhang","doi":"10.1093/hr/uhag173","DOIUrl":"10.1093/hr/uhag173","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 9","pages":"uhag173"},"PeriodicalIF":9.5,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13554799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148921885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 2A linker outperforms NAL10 in bicistronic translational efficiency for transgene stacking.","authors":"Yuan-Jie Deng, Rong-Rong Zhang, Ao-Qi Duan, Shan-Shan Tan, Shan-Shan Liu, Ai-Sheng Xiong","doi":"10.1093/hr/uhag170","DOIUrl":"10.1093/hr/uhag170","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 9","pages":"uhag170"},"PeriodicalIF":9.5,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13537015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148882820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanism of abscisic acid regulating bud-break through the NnTIFY10A/B-NnABI5 module in lotus.","authors":"Huiyan Jiang, Fengjun Liu, Xiaojing Liu, Ping Zhou, Guangyang Liu, Qijiang Jin, Yanjie Wang, Yingchun Xu","doi":"10.1093/hr/uhag125","DOIUrl":"10.1093/hr/uhag125","url":null,"abstract":"<p><p>The apical bud-break is an important stage in the regulation of lotus (<i>Nelumbo nucifera</i> Gaertn.) flowering. Phytohormones play a key role in the development of plant buds, but the molecular mechanisms underlying the crosstalk between different phytohormone signals, especially abscisic acid (ABA) and jasmonic acid (JA), in lotus bud-break remain unclear. In this study, we found that the exogenous application of ABA inhibited the lotus apical bud-break. In addition, the expression of the gene encoding ABSCISIC ACID INSENSITIVE 5 (NnABI5), a crucial regulator of ABA signaling, was gradually downregulated during lotus apical bud-break. The transient overexpression of <i>NnABI5</i> in lotus and heterologous expression in <i>Arabidopsis thaliana</i> (L.) Heynh. demonstrated that NnABI5 negatively regulates apical bud-break and seed germination. NnABI5 interacted with the JA pathway inhibitors NnTIF[F/Y] XG 10A/B (NnTIFY10A/B), reducing the binding ability of NnABI5 to response genes <i>EARLY METHIONINE-LABELED 1</i> (<i>NnEM1</i>) and <i>NnEM6</i>. In contrast, NnTIFY10A/B positively regulates apical bud break and seed germination. Notably, exogenous application of the JA biosynthesis inhibitor DIECA alleviated the inhibitory effect of ABA. <i>In vitro</i> protein degradation assays revealed that ABA could accelerate the degradation of NnTIFY10A/B. In summary, our data reveal the crosstalk of the JA and ABA signaling pathways in lotus apical bud-break, laying a theoretical foundation for understanding the regulation of flowering in this species.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag125"},"PeriodicalIF":9.5,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13404126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148610627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous editing of <i>CsLOB1</i> and <i>CsWRKY22</i> in citrus confers resistance to citrus canker.","authors":"Mengyuan Hou, Rongrong Qu, Mengting He, Xiangling Chen, Lanzhen Xu, Juanjuan Ma, Tiangang Lei, Yongrui He, Xiuping Zou","doi":"10.1093/hr/uhag137","DOIUrl":"10.1093/hr/uhag137","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag137"},"PeriodicalIF":9.5,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148686633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>StSAUR31</i> functions as a negative regulator of enzymatic browning in potato (<i>Solanum tuberosum</i> L.).","authors":"Zan Meng, Miaomiao Zhang, Yurong Ma, Guangcun Li, Shiyang Liu, Yu Cao, Yujie Niu, Qingqing Li, Qingguo Wang","doi":"10.1093/hr/uhag115","DOIUrl":"10.1093/hr/uhag115","url":null,"abstract":"<p><p>Enzymatic browning significantly affects the processing and quality maintenance of a wide range of horticultural produce. Identifying key regulators of browning is essential for elucidating its underlying mechanisms and developing effective mitigation strategies. In this study, transcriptomic comparison between potato cultivars with contrasting browning sensitivities identified a <i>small auxin-up RNA</i>, <i>StSAUR31</i>, as a potential regulator of auxin-mediated browning inhibition in potato. Functional analyses showed that overexpression of <i>StSAUR31</i> markedly reduced browning intensity and PPO activity, whereas knockout of <i>StSAUR31</i> produced the opposite phenotype. Correspondingly, StuPPO1 protein abundance decreased in <i>StSAUR31</i> overexpression lines and increased in knockout lines. Mechanistically, StSAUR31 physically interacted with StuPPO1 in an auxin-enhanced manner, partially altering its subcellular localization and reducing its accumulation in plastids. Additionally, <i>StSAUR31</i> downregulated <i>StuPPO1</i> expression, reduced endogenous free tyrosine levels, and enhanced antioxidant capacity. Collectively, these findings indicated that <i>StSAUR31</i> coordinately regulated PPO activity, substrate availability, and antioxidant capacity, thereby integrating multiple mechanisms to suppress enzymatic browning in potatoes. These results advance our understanding of the crosstalk between auxin signaling and enzymatic browning, providing new insights into the role of hormone signaling in postharvest quality regulation.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 7","pages":"uhag115"},"PeriodicalIF":9.5,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13326752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148378400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tomato SPAK and OFPs act as promising targets for fine-tuning fruit elongation by affecting cell division patterns at the proximal end.","authors":"Qiang Li, Bingbing Cai, Fubin Zhang, Yike Liu, Yunuo Chang, Xiao Du, Xupeng Jia, Esther van der Knaap, Qingyun Li","doi":"10.1093/hr/uhag123","DOIUrl":"10.1093/hr/uhag123","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag123"},"PeriodicalIF":9.5,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13401419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148594677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}