Food and Environmental Virology最新文献_第9页

The Emergence and Widespread Circulation of Enteric Viruses Throughout the COVID-19 Pandemic: A Wastewater-Based Evidence 新冠肺炎大流行期间肠道病毒的出现和广泛传播：基于废水的证据。

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-10-29 DOI: 10.1007/s12560-023-09566-z

Sheikh Ariful Hoque, Hiroyuki Saito, Wakako Akino, Tomohiro Kotaki, Shoko Okitsu, Yuko Onda, Takeshi Kobayashi, Tania Hossian, Pattara Khamrin, Kazushi Motomura, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima

{"title":"The Emergence and Widespread Circulation of Enteric Viruses Throughout the COVID-19 Pandemic: A Wastewater-Based Evidence","authors":"Sheikh Ariful Hoque, Hiroyuki Saito, Wakako Akino, Tomohiro Kotaki, Shoko Okitsu, Yuko Onda, Takeshi Kobayashi, Tania Hossian, Pattara Khamrin, Kazushi Motomura, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima","doi":"10.1007/s12560-023-09566-z","DOIUrl":"10.1007/s12560-023-09566-z","url":null,"abstract":"<div><p>Growing evidence shed light on the importance of wastewater-based epidemiology (WBE) during the pandemic, when the patients rarely visited the clinics despite the fact that the infections were still prevalent in the community as before. The abundance of infections in the community poses a constant threat of the emergence of new epidemic strains. Herein, we investigated enteric viruses in raw sewage water (SW) from Japan’s Tohoku region and compared them to those from the Kansai region to better understand the circulating strains and their distribution across communities during the COVID-19 pandemic. Raw SW was collected between 2019 and 2022, concentrated by polyethylene-glycol<i>-</i>precipitation method, and investigated for major AGE viruses by RT-PCR. Sequence-based analyses were used to assess genotypes and evolutionary relationships. The most commonly detected enteric virus was rotavirus A (RVA) at 63.8%, followed by astrovirus (AstV) at 61.1%, norovirus (NoV) GII and adenovirus (AdV) at 33.3%, sapovirus (SV) at 25.0%, enterovirus (EV) at 19.4%, and NoV GI at 13.9%. The highest prevalence (46.0%) was found in the spring. Importantly, enteric viruses did not decline during the pandemic. Rather, several strains like NoV GII.2, DS-1-like human G3 (equine) RVA, MLB1 AstV, and different F41 HAdV emerged throughout the pandemic and spread widely over the Tohoku and Kansai regions. Tohoku’s detection rate remained lower than that of the Kansai area (36 vs 58%). This study provides evidence for the emergence and spread of enteric viruses during the pandemic.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"342 - 354"},"PeriodicalIF":3.4,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71410088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of Gastroenteric Viruses in Wastewater from Cities in Uruguay 乌拉圭城市污水中胃肠道病毒的分子特征。

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-10-24 DOI: 10.1007/s12560-023-09567-y

Bortagaray Viviana, Salvo Matias, Mir Daiana, Colina Rodney, Victoria Matias

{"title":"Molecular Characterization of Gastroenteric Viruses in Wastewater from Cities in Uruguay","authors":"Bortagaray Viviana, Salvo Matias, Mir Daiana, Colina Rodney, Victoria Matias","doi":"10.1007/s12560-023-09567-y","DOIUrl":"10.1007/s12560-023-09567-y","url":null,"abstract":"<div><p>Group A Rotavirus, Human Astrovirus, and Norovirus (RVA, HAstV, and NoV) are recognized as the major causative agents of acute gastroenteritis in children and adults worldwide. The aim of this study was to determine the prevalence and molecular epidemiology of RVA, HAstV, and NoV in wastewater from three cities in Uruguay. Thirty-six samples from Bella Unión, Salto, and Fray Bentos cities were analyzed using quantitative and qualitative PCR. RVA was the most frequently detected virus (50%), followed by HAstV (39%), NoV GII (36%), and NoV GI (25%). RVA strains were characterized as P[8] and G3 based on the VP4 and VP7 genes, respectively. Among NoV-positive samples, genotypes GI.2, GI.3, GI.5, GI.6, GI.7, GII.2, GII.6, and GII.4 were detected, and only one HAstV genotype (MLB1) was found. Our wastewater-based epidemiological approach provides a snapshot of the overall genetic diversity of these viruses in three cities of the Uruguay River basin during 2017–2018. These findings reinforce the importance of this environmental surveillance tool for monitoring epidemiological trends of enteric viruses circulating in the population, which can be used to guide public health intervention.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"318 - 330"},"PeriodicalIF":3.4,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wastewater-based Epidemiology and SARS-CoV-2: Variant Trends in the Apulia Region (Southern Italy) and Effect of Some Environmental Parameters 基于废水的流行病学和严重急性呼吸系统综合征冠状病毒2型：阿普利亚地区（意大利南部）的变异趋势和一些环境参数的影响。

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-09-21 DOI: 10.1007/s12560-023-09565-0

Francesco Triggiano, Osvalda De Giglio, Francesca Apollonio, Silvia Brigida, Fabrizio Fasano, Pamela Mancini, Giusy Bonanno Ferraro, Carolina Veneri, Giuseppina La Rosa, Elisabetta Suffredini, Luca Lucentini, Nicola Ungaro, Giuseppe Di Vittorio, Onofrio Mongelli, Nelhudoff Albano, Maria Teresa Montagna

{"title":"Wastewater-based Epidemiology and SARS-CoV-2: Variant Trends in the Apulia Region (Southern Italy) and Effect of Some Environmental Parameters","authors":"Francesco Triggiano, Osvalda De Giglio, Francesca Apollonio, Silvia Brigida, Fabrizio Fasano, Pamela Mancini, Giusy Bonanno Ferraro, Carolina Veneri, Giuseppina La Rosa, Elisabetta Suffredini, Luca Lucentini, Nicola Ungaro, Giuseppe Di Vittorio, Onofrio Mongelli, Nelhudoff Albano, Maria Teresa Montagna","doi":"10.1007/s12560-023-09565-0","DOIUrl":"10.1007/s12560-023-09565-0","url":null,"abstract":"<div><p>During the COVID-19 pandemic, wastewater monitoring has been used to monitor the levels of SARS-CoV-2 RNA entering the sewerage system. In Italy, the Istituto Superiore di Sanità coordinated the SARI project (Sorveglianza Ambientale Reflue in Italia) to detect SARS-CoV-2 and its variants. In this study, the concentration of SARS-CoV-2 and its variants in raw wastewater against COVID-19 cases was evaluated together with the effect of temperature and precipitation on virus spread. We validated a predictive model, proposed by De Giglio et al., 2021, to establish the number of COVID-19 cases/100,000 inhabitants. A receiver operating characteristic curve model was applied to predict the number of COVID-19 cases and Poisson regression was applied to study the effect of temperature and rainfall on viral load. In Apulia, from October 2021 to December 2022, we analyzed 1041 samples, of which 985 (94.6%) tested positive for SARS-CoV-2. Median atmospheric temperature was inversely proportional to viral load in wastewater; no correlation was found with precipitation. The predictive model confirmed that at least 11 cases/100,000 inhabitants would occur in the 15 days following the detection of the virus in wastewater. Environmental surveillance of SARS-CoV-2 can be used to map the virus and its variants.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"331 - 341"},"PeriodicalIF":3.4,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-023-09565-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41097302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Seroepidemiological Investigation of Hepatitis E Virus Infection in Backyard Pigs from Northeastern India: Prevalence and Associated Risk Factors 印度东北部户养猪戊型肝炎病毒感染的首次血清流行病学调查:流行率及相关危险因素

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-09-08 DOI: 10.1007/s12560-023-09564-1

A. A. P. Milton, Samir Das, S. Ghatak, K. Srinivas, M. Angappan, M. C. B. Prasad, L. Wahlang, G. Bhuvana Priya, Sabia Khan, Blessa Sailo, Lalhruaipuii, Mahak Singh, G. B. Garam, A. Sen

{"title":"First Seroepidemiological Investigation of Hepatitis E Virus Infection in Backyard Pigs from Northeastern India: Prevalence and Associated Risk Factors","authors":"A. A. P. Milton, Samir Das, S. Ghatak, K. Srinivas, M. Angappan, M. C. B. Prasad, L. Wahlang, G. Bhuvana Priya, Sabia Khan, Blessa Sailo,  Lalhruaipuii, Mahak Singh, G. B. Garam, A. Sen","doi":"10.1007/s12560-023-09564-1","DOIUrl":"10.1007/s12560-023-09564-1","url":null,"abstract":"<div><p>Hepatitis E virus (HEV) is the leading cause of acute viral hepatitis globally, with zoonotic potential, and pigs are considered the major reservoir. To determine the seroprevalence of HEV infection in pigs reared in backyard conditions in the northeastern region of India, blood samples were collected from 400 pigs from five northeastern states (80 samples from each state) and tested for IgG antibodies against HEV using an ELISA assay. Questionnaires on farm characteristics and management practices were completed, and risk factors associated with HEV were studied using univariate and multivariate analysis. The apparent seroprevalence of HEV infection was 51% (46.1–55.9, 95% CI), with a true prevalence of 52.98% (47.22–58.75, 95% CI). The risk factors significantly associated with higher HEV seropositivity were as follows: lack of disinfection (OR 4.65), feeding swill (restaurant and bakery waste) (OR 2.55), failure to follow the all-in-all-out production system (OR 3.47), and medium holding size (OR 9.83), which refers to mixed rearing of younger and older age groups. This study demonstrates that HEV is widespread among pigs reared in northeastern India. The risk factor analysis conducted in this study provides valuable insights into the prevalence of HEV in the region.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"307 - 317"},"PeriodicalIF":3.4,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10239391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-circulation of Hepatitis E Virus (HEV) Genotype 3 and Moose-HEV-Like Strains in Free-Ranging-Spotted Deer (Axis axis) in Uruguay 乌拉圭自由放养的斑鹿(轴轴)中戊型肝炎病毒(HEV)基因3型和驼鹿-HEV样毒株的共循环

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-08-29 DOI: 10.1007/s12560-023-09563-2

Florencia Cancela, Alexandra Cravino, Romina Icasuriaga, Pablo González, Federico Bentancor, Carmen Leizagoyen, César Echaides, Irene Ferreiro, Andrés Cabrera, Juan Arbiza, Santiago Mirazo

{"title":"Co-circulation of Hepatitis E Virus (HEV) Genotype 3 and Moose-HEV-Like Strains in Free-Ranging-Spotted Deer (Axis axis) in Uruguay","authors":"Florencia Cancela, Alexandra Cravino, Romina Icasuriaga, Pablo González, Federico Bentancor, Carmen Leizagoyen, César Echaides, Irene Ferreiro, Andrés Cabrera, Juan Arbiza, Santiago Mirazo","doi":"10.1007/s12560-023-09563-2","DOIUrl":"10.1007/s12560-023-09563-2","url":null,"abstract":"<div><p>Hepatitis E caused by hepatitis E virus (HEV) is considered an emerging foodborne zoonosis in industrialized, non-endemic countries. Domestic pigs and wild boars are considered the main reservoir of HEV. However, HEV can also infect an ever-expanding host range of animals, but they exact role in transmitting the virus to other species or humans is mostly unknown. In this work, we investigated the spread of HEV in free-living and captive spotted deer (<i>Axis axis</i>) from Uruguay in a 2-year period (2020–2022) and examined the role of this invasive species as a new potential reservoir of the virus. In addition, with the aim to gain new insights into viral ecology in the context of One Health, by using camera trapping, we identified and quantified temporal and spatial coexistence of spotted deer, wild boars, and cattle. In free-living animals, we detected an anti-HEV seropositivity of 11.1% (6/54). HEV infection and viral excretion in feces were assessed by RT-PCR. Thirteen of 19 samples (68.4%) had HEV RNA. Six samples were amplified using a broadly reactive RT-PCR and sequenced. No captive animal showed evidence of HEV infection. Additionally, HEV RNA was detected in a freshwater pond shared by these species. Phylogenetic and <i>p</i>-distance analysis revealed that zoonotic HEV genotype 3 strains circulate together with unclassified variants related to moose HEV whose potential risk of transmission to humans and other domestic and wild animals is unknown. The data presented here suggest that spotted deer (<i>A. axis</i>) may be a novel host for zoonotic HEV strains.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"281 - 291"},"PeriodicalIF":3.4,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10467717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First Detection and Characterization of Hepatitis E Virus in Sewage Samples in Cameroon 喀麦隆污水样本中首次检测和鉴定戊型肝炎病毒

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-08-09 DOI: 10.1007/s12560-023-09562-3

Modiyinji Abdou Fatawou, Monamele Gwladys Chavely, Moumbeket Yifomnjou Moise Henri, Kamga Njile Daniel, Endegue Zanga Marie Claire, Njouom Richard

{"title":"First Detection and Characterization of Hepatitis E Virus in Sewage Samples in Cameroon","authors":"Modiyinji Abdou Fatawou, Monamele Gwladys Chavely, Moumbeket Yifomnjou Moise Henri, Kamga Njile Daniel, Endegue Zanga Marie Claire, Njouom Richard","doi":"10.1007/s12560-023-09562-3","DOIUrl":"10.1007/s12560-023-09562-3","url":null,"abstract":"<div><p>Hepatitis E virus (HEV) represents an important public health concern in many developing countries, including Africa. Transmission of HEV to humans by contaminated drinking water is the most important mode of transmission in low- and middle-income countries. This study aimed to assess the presence of HEV in the environment in Cameroon through molecular analysis of sewage samples. Retrospectively, a total of 157 sewage samples collected between January 2018 and December 2019 were randomly selected and analyzed by molecular techniques to detect and characterize the HEV followed by sequencing and phylogenetic analysis. Three samples (1.9%) collected from North, Far North, and Adamawa regions were positive by real-time reverse transcription polymerization chain reaction. Among these, 2 samples were positive for HEV ribonucleic acid by nested reverse transcription polymerization chain reaction and only one yielded a good sequencing product. Phylogenetic analysis of this unique HEV strain showed that this HEV strain belonged to genotype 3, subtype 3a, and clustered with swine HEV strains from Cameroon, Argentina, and the USA. This study provides preliminary data on the circulation of HEV in wastewater in Cameroon. Further studies will be needed to assess the overall situation in Cameroon.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 3","pages":"255 - 261"},"PeriodicalIF":3.4,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6709846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Hepatitis A RNA, Hepatitis E RNA, Human Adenovirus F DNA, and Norovirus RNA in Fresh and Frozen Berry Products at Point of Retail in Ireland 爱尔兰零售店新鲜和冷冻浆果产品中甲型肝炎RNA、戊型肝炎RNA、人腺病毒F DNA和诺如病毒RNA的检测

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-08-01 DOI: 10.1007/s12560-023-09561-4

Charlene Bennett, Kevin Hunt, Francis Butler, Sinead Keaveney, Séamus Fanning, Cillian De Gascun, Suzie Coughlan, Joanne O’Gorman

{"title":"Detection of Hepatitis A RNA, Hepatitis E RNA, Human Adenovirus F DNA, and Norovirus RNA in Fresh and Frozen Berry Products at Point of Retail in Ireland","authors":"Charlene Bennett, Kevin Hunt, Francis Butler, Sinead Keaveney, Séamus Fanning, Cillian De Gascun, Suzie Coughlan, Joanne O’Gorman","doi":"10.1007/s12560-023-09561-4","DOIUrl":"10.1007/s12560-023-09561-4","url":null,"abstract":"<div><p>Soft fruits are at particular risk of contamination with enteric viruses such as Hepatitis A virus (HAV), Hepatitis E Virus (HEV), Norovirus (NoV), Human Adenovirus (HAdV) and Sapovirus (SaV). The aim of this study was to investigate, for the first time, the presence of these biological agents in ready to eat (RTE) berries at point of retail in Ireland. A sampling strategy was designed in which RTE fresh and frozen strawberries and raspberries were purchased from five retailers between May and October 2018. Reverse Transcriptase Polymerase Chain Reaction (RT-qPCR) assays for HEV RNA, Nov RNA, SaV RNA, and human Adenovirus species F DNA (HAdV-F) were performed on 239 samples (25g portions). Viral nucleic acid was present in 6.7% (<i>n</i> = 16) of samples tested as follows: HAV RNA (<i>n</i> = 5), HAdV-F DNA (<i>n</i> = 5), HEV RNA (<i>n</i> = 3) and NoV GII RNA (<i>n </i>= 3). Sapovirus RNA was not detected in any product. No significant differences were found between berry type, fresh/frozen status, or supermarket source. This study suggests a risk that exists across all retail outlets however only low levels of nucleic acid ranging from 0 to 16 genome copies/g were present. Although these findings may reflect non-viable/non-infectious virus the continued provision of risk mitigation advice to consumers is warranted and further work is required to ensure control measures to reduce contamination are implemented and enforced.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 3","pages":"246 - 254"},"PeriodicalIF":3.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-023-09561-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6709847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hepatitis E Virus in the Iberian Peninsula: A Systematic Review 伊比利亚半岛戊型肝炎病毒:系统综述

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-07-11 DOI: 10.1007/s12560-023-09560-5

Sérgio Santos-Silva, Danny Franciele da Silva Dias Moraes, Pedro López-López, António Rivero-Juarez, João R. Mesquita, Maria São José Nascimento

{"title":"Hepatitis E Virus in the Iberian Peninsula: A Systematic Review","authors":"Sérgio Santos-Silva, Danny Franciele da Silva Dias Moraes, Pedro López-López, António Rivero-Juarez, João R. Mesquita, Maria São José Nascimento","doi":"10.1007/s12560-023-09560-5","DOIUrl":"10.1007/s12560-023-09560-5","url":null,"abstract":"<div><p>One of the most frequent causes of acute viral hepatitis is hepatitis E virus (HEV) causing 20 million infections worldwide each year and 44,000 deaths. Studies on HEV in the Iberian Peninsula have been increasing through time with HEV infection being identified in humans and animals. The aim of the present systematic review was to compile and evaluate all the published data on HEV from studies performed in humans, animals and environmental samples in the Iberian Peninsula. The electronic databases Mendeley, PubMed, Scopus, and Web of Science were thoroughly searched, and research published up until February 01, 2023 were included. Resulting in a total of 151 eligible papers by full reading and application of PRISMA exclusion/inclusion criteria. Overall, the present review shows that several HEV genotypes, namely HEV-1, 3, 4, and 6 as well as <i>Rocahepevirus</i>, are circulating in humans, animals, and in the environment in the Iberian Peninsula. HEV-3 was the most common genotype circulating in humans in Portugal and Spain, as expected for developed countries, with HEV-1 only being detected in travelers and emigrants from HEV endemic regions. Spain is the biggest pork producer in Europe and given the high circulation of HEV in pigs, with HEV-3 being primarily associated to zoonotic transmission through consumption of swine meat and meat products, in our opinion, the introduction of an HEV surveillance system in swine and inclusion of HEV in diagnostic routines for acute and chronic human hepatitis would be important. Additionally, we propose that establishing a monitoring mechanism for HEV is crucial in order to gain a comprehensive understanding of the prevalence of this illness and the various strains present in the Iberian Peninsula, as well as their potential impact on public health.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 3","pages":"193 - 211"},"PeriodicalIF":3.4,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-023-09560-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6709873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inactivation of SARS-CoV-2 in Water by Chlorination 氯化法对水中SARS-CoV-2的灭活作用

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-07-08 DOI: 10.1007/s12560-023-09559-y

Samendra Sherchan, Luisa A. Ikner, Charles P. Gerba

引用次数: 0

Genome Characterization of the Novel Lytic Enterobacter cloacae Phage vB_EclM_Q7622 新型泄殖腔肠杆菌噬菌体vB_EclM_Q7622的基因组特征

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-06-12 DOI: 10.1007/s12560-023-09558-z

Shilin Tan, Shixuan Huang, Zekun Liu, Moutong Chen, Hanfang Chen, Qinghua Ye, Shi Wu, Xiaojuan Yang, Shuhong Zhang, Jumei Zhang, Qingping Wu, Meiyan Yang

{"title":"Genome Characterization of the Novel Lytic Enterobacter cloacae Phage vB_EclM_Q7622","authors":"Shilin Tan, Shixuan Huang, Zekun Liu, Moutong Chen, Hanfang Chen, Qinghua Ye, Shi Wu, Xiaojuan Yang, Shuhong Zhang, Jumei Zhang, Qingping Wu, Meiyan Yang","doi":"10.1007/s12560-023-09558-z","DOIUrl":"10.1007/s12560-023-09558-z","url":null,"abstract":"<div><p><i>Enterobacter cloacae</i> is a widespread opportunistic pathogen that causes urinary tract infection. The abuse of antibiotics enabled multidrug-resistant strains to spread. Bacteriophage therapy is a naturally, safe, and efficient alternative treatment technology against multi-resistant bacteria. In this study, a virulent phage vB_EclM_Q7622 (Q7622) was isolated from the sewage of Jiangcun poultry market in Guangzhou city. Transmission electron microscopy indicated that Q7622 had an icosahedral head (97.8 ± 5.6 nm in diameter) and a short, contractile tail (113.7 ± 4.5 nm). Its double-stranded DNA genome is composed of 173,871 bp with a GC content of 40.02%. It possesses 297 open reading frames and 9 tRNAs. No known virulence and resistance genes were detected, indicated that phage Q7622 could be used for pathogens prevention and control safely. Comparative genomic and phylogenetic analysis showed that Q7622 was highly similar to the phages vB_EclM_CIP9 and vB_EhoM-IME523. The highest nucleotide similarity between Q7622 and the similar phages in NCBI calculated by pyANI and VIRIDIC were 94.9% and 89.1% with vB_EhoM-IME523 respectively, below 95%. Thus, according to the result of nucleotide similarity calculation results, Q7622 was a novel virulent <i>Enterobacter cloacae</i> phage strain of the genus <i>Kanagawavirus</i>.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 3","pages":"236 - 245"},"PeriodicalIF":3.4,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6709823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0