Food and Environmental Virology最新文献_第8页

Sensitivity Evaluation of Enveloped and Non-enveloped Viruses to Ethanol Using Machine Learning: A Systematic Review 用机器学习评价包膜和非包膜病毒对乙醇的敏感性:系统综述。

IF 4.1 2区农林科学

Food and Environmental Virology Pub Date : 2023-12-05 DOI: 10.1007/s12560-023-09571-2

Aken Puti Wanguyun, Wakana Oishi, Daisuke Sano

{"title":"Sensitivity Evaluation of Enveloped and Non-enveloped Viruses to Ethanol Using Machine Learning: A Systematic Review","authors":"Aken Puti Wanguyun, Wakana Oishi, Daisuke Sano","doi":"10.1007/s12560-023-09571-2","DOIUrl":"10.1007/s12560-023-09571-2","url":null,"abstract":"<div><p>Viral diseases are a severe public health issue worldwide. During the coronavirus pandemic, the use of alcohol-based sanitizers was recommended by WHO. Enveloped viruses are sensitive to ethanol, whereas non-enveloped viruses are considerably less sensitive. However, no quantitative analysis has been conducted to determine virus ethanol sensitivity and the important variables influencing the inactivation of viruses to ethanol. This study aimed to determine viruses’ sensitivity to ethanol and the most important variables influencing the inactivation of viruses exposed to ethanol based on machine learning. We examined 37 peer-reviewed articles through a systematic search. Quantitative analysis was employed using a decision tree and random forest algorithms. Based on the decision tree, enveloped viruses required around ≥ 35% ethanol with an average contact time of at least 1 min, which reduced the average viral load by 4 log<sub>10</sub>. In non-enveloped viruses with and without organic matter, ≥ 77.50% and ≥ 65% ethanol with an extended contact time of ≥ 2 min were required for a 4 log<sub>10</sub> viral reduction, respectively. Important variables were assessed using a random forest based on the percentage increases in mean square error (%IncMSE) and node purity (%IncNodePurity). Ethanol concentration was a more important variable with a higher %IncMSE and %IncNodePurity than contact time for the inactivation of enveloped and non-enveloped viruses with the available organic matter. Because specific guidelines for virus inactivation by ethanol are lacking, data analysis using machine learning is essential to gain insight from certain datasets. We provide new knowledge for determining guideline values related to the selection of ethanol concentration and contact time that effectively inactivate viruses.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"16 1","pages":"1 - 13"},"PeriodicalIF":4.1,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10963467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138481705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabarcoding of Hepatitis E Virus Genotype 3 and Norovirus GII from Wastewater Samples in England Using Nanopore Sequencing 应用纳米孔测序法对英国废水样本中戊型肝炎病毒基因型3和诺如病毒GII的代谢编码。

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-11-01 DOI: 10.1007/s12560-023-09569-w

Samantha Treagus, James Lowther, Ben Longdon, William Gaze, Craig Baker-Austin, David Ryder, Frederico M. Batista

{"title":"Metabarcoding of Hepatitis E Virus Genotype 3 and Norovirus GII from Wastewater Samples in England Using Nanopore Sequencing","authors":"Samantha Treagus, James Lowther, Ben Longdon, William Gaze, Craig Baker-Austin, David Ryder, Frederico M. Batista","doi":"10.1007/s12560-023-09569-w","DOIUrl":"10.1007/s12560-023-09569-w","url":null,"abstract":"<div><p>Norovirus is one of the largest causes of gastroenteritis worldwide, and Hepatitis E virus (HEV) is an emerging pathogen that has become the most dominant cause of acute viral hepatitis in recent years. The presence of norovirus and HEV has been reported within wastewater in many countries previously. Here we used amplicon deep sequencing (metabarcoding) to identify norovirus and HEV strains in wastewater samples from England collected in 2019 and 2020. For HEV, we sequenced a fragment of the RNA-dependent RNA polymerase (RdRp) gene targeting genotype three strains. For norovirus, we sequenced the 5′ portion of the major capsid protein gene (VP1) of genogroup II strains. Sequencing of the wastewater samples revealed eight different genotypes of norovirus GII (GII.2, GII.3, GII.4, GII.6, GII.7, GII.9, GII.13 and GII.17). Genotypes GII.3 and GII.4 were the most commonly found. The HEV metabarcoding assay was able to identify HEV genotype 3 strains in some samples with a very low viral concentration determined by RT-qPCR. Analysis showed that most HEV strains found in influent wastewater were typed as G3c and G3e and were likely to have originated from humans or swine. However, the small size of the HEV nested PCR amplicon could cause issues with typing, and so this method is more appropriate for samples with high CTs where methods targeting longer genomic regions are unlikely to be successful. This is the first report of HEV RNA in wastewater in England. This study demonstrates the utility of wastewater sequencing and the need for wider surveillance of norovirus and HEV within host species and environments.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"292 - 306"},"PeriodicalIF":3.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7615314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71419547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral Natural Products, Their Mechanisms of Action and Potential Applications as Sanitizers and Disinfectants 抗病毒天然产物，它们的作用机制以及作为消毒剂和消毒剂的潜在应用。

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-10-31 DOI: 10.1007/s12560-023-09568-x

Stephanie A. Boone, M. Khalid Ijaz, Kelly R. Bright, Norma Patricia Silva-Beltran, Raymond W. Nims, Julie McKinney, Charles P. Gerba

{"title":"Antiviral Natural Products, Their Mechanisms of Action and Potential Applications as Sanitizers and Disinfectants","authors":"Stephanie A. Boone, M. Khalid Ijaz, Kelly R. Bright, Norma Patricia Silva-Beltran, Raymond W. Nims, Julie McKinney, Charles P. Gerba","doi":"10.1007/s12560-023-09568-x","DOIUrl":"10.1007/s12560-023-09568-x","url":null,"abstract":"<div><p>Plant extracts, natural products and plant oils contain natural virucidal actives that can be used to replace active ingredients in commercial sanitizers and disinfectants. This review focuses on the virucidal mechanisms of natural substances that may exhibit potential for indoor air and fomite disinfection. Review of scientific studies indicates: (1) most natural product studies use crude extracts and do not isolate or identify exact active antiviral substances; (2) many natural product studies contain unclear explanations of virucidal mechanisms of action; (3) natural product evaluations of virucidal activity should include methods that validate efficacy under standardized disinfectant testing procedures (e.g., carrier tests on applicable surfaces or activity against aerosolized viruses, etc.). The development of natural product disinfectants requires a better understanding of the mechanisms of action (MOA), chemical profiles, compound specificities, activity spectra, and the chemical formulations required for maximum activity. Combinations of natural antiviral substances and possibly the addition of synthetic compounds might be needed to increase inactivation of a broader spectrum of viruses, thereby providing the required efficacy for surface and air disinfection.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"265 - 280"},"PeriodicalIF":3.4,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71419546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Emergence and Widespread Circulation of Enteric Viruses Throughout the COVID-19 Pandemic: A Wastewater-Based Evidence 新冠肺炎大流行期间肠道病毒的出现和广泛传播：基于废水的证据。

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-10-29 DOI: 10.1007/s12560-023-09566-z

Sheikh Ariful Hoque, Hiroyuki Saito, Wakako Akino, Tomohiro Kotaki, Shoko Okitsu, Yuko Onda, Takeshi Kobayashi, Tania Hossian, Pattara Khamrin, Kazushi Motomura, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima

{"title":"The Emergence and Widespread Circulation of Enteric Viruses Throughout the COVID-19 Pandemic: A Wastewater-Based Evidence","authors":"Sheikh Ariful Hoque, Hiroyuki Saito, Wakako Akino, Tomohiro Kotaki, Shoko Okitsu, Yuko Onda, Takeshi Kobayashi, Tania Hossian, Pattara Khamrin, Kazushi Motomura, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima","doi":"10.1007/s12560-023-09566-z","DOIUrl":"10.1007/s12560-023-09566-z","url":null,"abstract":"<div><p>Growing evidence shed light on the importance of wastewater-based epidemiology (WBE) during the pandemic, when the patients rarely visited the clinics despite the fact that the infections were still prevalent in the community as before. The abundance of infections in the community poses a constant threat of the emergence of new epidemic strains. Herein, we investigated enteric viruses in raw sewage water (SW) from Japan’s Tohoku region and compared them to those from the Kansai region to better understand the circulating strains and their distribution across communities during the COVID-19 pandemic. Raw SW was collected between 2019 and 2022, concentrated by polyethylene-glycol<i>-</i>precipitation method, and investigated for major AGE viruses by RT-PCR. Sequence-based analyses were used to assess genotypes and evolutionary relationships. The most commonly detected enteric virus was rotavirus A (RVA) at 63.8%, followed by astrovirus (AstV) at 61.1%, norovirus (NoV) GII and adenovirus (AdV) at 33.3%, sapovirus (SV) at 25.0%, enterovirus (EV) at 19.4%, and NoV GI at 13.9%. The highest prevalence (46.0%) was found in the spring. Importantly, enteric viruses did not decline during the pandemic. Rather, several strains like NoV GII.2, DS-1-like human G3 (equine) RVA, MLB1 AstV, and different F41 HAdV emerged throughout the pandemic and spread widely over the Tohoku and Kansai regions. Tohoku’s detection rate remained lower than that of the Kansai area (36 vs 58%). This study provides evidence for the emergence and spread of enteric viruses during the pandemic.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"342 - 354"},"PeriodicalIF":3.4,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71410088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of Gastroenteric Viruses in Wastewater from Cities in Uruguay 乌拉圭城市污水中胃肠道病毒的分子特征。

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-10-24 DOI: 10.1007/s12560-023-09567-y

Bortagaray Viviana, Salvo Matias, Mir Daiana, Colina Rodney, Victoria Matias

{"title":"Molecular Characterization of Gastroenteric Viruses in Wastewater from Cities in Uruguay","authors":"Bortagaray Viviana, Salvo Matias, Mir Daiana, Colina Rodney, Victoria Matias","doi":"10.1007/s12560-023-09567-y","DOIUrl":"10.1007/s12560-023-09567-y","url":null,"abstract":"<div><p>Group A Rotavirus, Human Astrovirus, and Norovirus (RVA, HAstV, and NoV) are recognized as the major causative agents of acute gastroenteritis in children and adults worldwide. The aim of this study was to determine the prevalence and molecular epidemiology of RVA, HAstV, and NoV in wastewater from three cities in Uruguay. Thirty-six samples from Bella Unión, Salto, and Fray Bentos cities were analyzed using quantitative and qualitative PCR. RVA was the most frequently detected virus (50%), followed by HAstV (39%), NoV GII (36%), and NoV GI (25%). RVA strains were characterized as P[8] and G3 based on the VP4 and VP7 genes, respectively. Among NoV-positive samples, genotypes GI.2, GI.3, GI.5, GI.6, GI.7, GII.2, GII.6, and GII.4 were detected, and only one HAstV genotype (MLB1) was found. Our wastewater-based epidemiological approach provides a snapshot of the overall genetic diversity of these viruses in three cities of the Uruguay River basin during 2017–2018. These findings reinforce the importance of this environmental surveillance tool for monitoring epidemiological trends of enteric viruses circulating in the population, which can be used to guide public health intervention.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"318 - 330"},"PeriodicalIF":3.4,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wastewater-based Epidemiology and SARS-CoV-2: Variant Trends in the Apulia Region (Southern Italy) and Effect of Some Environmental Parameters 基于废水的流行病学和严重急性呼吸系统综合征冠状病毒2型：阿普利亚地区（意大利南部）的变异趋势和一些环境参数的影响。

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-09-21 DOI: 10.1007/s12560-023-09565-0

Francesco Triggiano, Osvalda De Giglio, Francesca Apollonio, Silvia Brigida, Fabrizio Fasano, Pamela Mancini, Giusy Bonanno Ferraro, Carolina Veneri, Giuseppina La Rosa, Elisabetta Suffredini, Luca Lucentini, Nicola Ungaro, Giuseppe Di Vittorio, Onofrio Mongelli, Nelhudoff Albano, Maria Teresa Montagna

{"title":"Wastewater-based Epidemiology and SARS-CoV-2: Variant Trends in the Apulia Region (Southern Italy) and Effect of Some Environmental Parameters","authors":"Francesco Triggiano, Osvalda De Giglio, Francesca Apollonio, Silvia Brigida, Fabrizio Fasano, Pamela Mancini, Giusy Bonanno Ferraro, Carolina Veneri, Giuseppina La Rosa, Elisabetta Suffredini, Luca Lucentini, Nicola Ungaro, Giuseppe Di Vittorio, Onofrio Mongelli, Nelhudoff Albano, Maria Teresa Montagna","doi":"10.1007/s12560-023-09565-0","DOIUrl":"10.1007/s12560-023-09565-0","url":null,"abstract":"<div><p>During the COVID-19 pandemic, wastewater monitoring has been used to monitor the levels of SARS-CoV-2 RNA entering the sewerage system. In Italy, the Istituto Superiore di Sanità coordinated the SARI project (Sorveglianza Ambientale Reflue in Italia) to detect SARS-CoV-2 and its variants. In this study, the concentration of SARS-CoV-2 and its variants in raw wastewater against COVID-19 cases was evaluated together with the effect of temperature and precipitation on virus spread. We validated a predictive model, proposed by De Giglio et al., 2021, to establish the number of COVID-19 cases/100,000 inhabitants. A receiver operating characteristic curve model was applied to predict the number of COVID-19 cases and Poisson regression was applied to study the effect of temperature and rainfall on viral load. In Apulia, from October 2021 to December 2022, we analyzed 1041 samples, of which 985 (94.6%) tested positive for SARS-CoV-2. Median atmospheric temperature was inversely proportional to viral load in wastewater; no correlation was found with precipitation. The predictive model confirmed that at least 11 cases/100,000 inhabitants would occur in the 15 days following the detection of the virus in wastewater. Environmental surveillance of SARS-CoV-2 can be used to map the virus and its variants.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"331 - 341"},"PeriodicalIF":3.4,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-023-09565-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41097302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Seroepidemiological Investigation of Hepatitis E Virus Infection in Backyard Pigs from Northeastern India: Prevalence and Associated Risk Factors 印度东北部户养猪戊型肝炎病毒感染的首次血清流行病学调查:流行率及相关危险因素

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-09-08 DOI: 10.1007/s12560-023-09564-1

A. A. P. Milton, Samir Das, S. Ghatak, K. Srinivas, M. Angappan, M. C. B. Prasad, L. Wahlang, G. Bhuvana Priya, Sabia Khan, Blessa Sailo, Lalhruaipuii, Mahak Singh, G. B. Garam, A. Sen

{"title":"First Seroepidemiological Investigation of Hepatitis E Virus Infection in Backyard Pigs from Northeastern India: Prevalence and Associated Risk Factors","authors":"A. A. P. Milton, Samir Das, S. Ghatak, K. Srinivas, M. Angappan, M. C. B. Prasad, L. Wahlang, G. Bhuvana Priya, Sabia Khan, Blessa Sailo,  Lalhruaipuii, Mahak Singh, G. B. Garam, A. Sen","doi":"10.1007/s12560-023-09564-1","DOIUrl":"10.1007/s12560-023-09564-1","url":null,"abstract":"<div><p>Hepatitis E virus (HEV) is the leading cause of acute viral hepatitis globally, with zoonotic potential, and pigs are considered the major reservoir. To determine the seroprevalence of HEV infection in pigs reared in backyard conditions in the northeastern region of India, blood samples were collected from 400 pigs from five northeastern states (80 samples from each state) and tested for IgG antibodies against HEV using an ELISA assay. Questionnaires on farm characteristics and management practices were completed, and risk factors associated with HEV were studied using univariate and multivariate analysis. The apparent seroprevalence of HEV infection was 51% (46.1–55.9, 95% CI), with a true prevalence of 52.98% (47.22–58.75, 95% CI). The risk factors significantly associated with higher HEV seropositivity were as follows: lack of disinfection (OR 4.65), feeding swill (restaurant and bakery waste) (OR 2.55), failure to follow the all-in-all-out production system (OR 3.47), and medium holding size (OR 9.83), which refers to mixed rearing of younger and older age groups. This study demonstrates that HEV is widespread among pigs reared in northeastern India. The risk factor analysis conducted in this study provides valuable insights into the prevalence of HEV in the region.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"307 - 317"},"PeriodicalIF":3.4,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10239391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-circulation of Hepatitis E Virus (HEV) Genotype 3 and Moose-HEV-Like Strains in Free-Ranging-Spotted Deer (Axis axis) in Uruguay 乌拉圭自由放养的斑鹿(轴轴)中戊型肝炎病毒(HEV)基因3型和驼鹿-HEV样毒株的共循环

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-08-29 DOI: 10.1007/s12560-023-09563-2

Florencia Cancela, Alexandra Cravino, Romina Icasuriaga, Pablo González, Federico Bentancor, Carmen Leizagoyen, César Echaides, Irene Ferreiro, Andrés Cabrera, Juan Arbiza, Santiago Mirazo

{"title":"Co-circulation of Hepatitis E Virus (HEV) Genotype 3 and Moose-HEV-Like Strains in Free-Ranging-Spotted Deer (Axis axis) in Uruguay","authors":"Florencia Cancela, Alexandra Cravino, Romina Icasuriaga, Pablo González, Federico Bentancor, Carmen Leizagoyen, César Echaides, Irene Ferreiro, Andrés Cabrera, Juan Arbiza, Santiago Mirazo","doi":"10.1007/s12560-023-09563-2","DOIUrl":"10.1007/s12560-023-09563-2","url":null,"abstract":"<div><p>Hepatitis E caused by hepatitis E virus (HEV) is considered an emerging foodborne zoonosis in industrialized, non-endemic countries. Domestic pigs and wild boars are considered the main reservoir of HEV. However, HEV can also infect an ever-expanding host range of animals, but they exact role in transmitting the virus to other species or humans is mostly unknown. In this work, we investigated the spread of HEV in free-living and captive spotted deer (<i>Axis axis</i>) from Uruguay in a 2-year period (2020–2022) and examined the role of this invasive species as a new potential reservoir of the virus. In addition, with the aim to gain new insights into viral ecology in the context of One Health, by using camera trapping, we identified and quantified temporal and spatial coexistence of spotted deer, wild boars, and cattle. In free-living animals, we detected an anti-HEV seropositivity of 11.1% (6/54). HEV infection and viral excretion in feces were assessed by RT-PCR. Thirteen of 19 samples (68.4%) had HEV RNA. Six samples were amplified using a broadly reactive RT-PCR and sequenced. No captive animal showed evidence of HEV infection. Additionally, HEV RNA was detected in a freshwater pond shared by these species. Phylogenetic and <i>p</i>-distance analysis revealed that zoonotic HEV genotype 3 strains circulate together with unclassified variants related to moose HEV whose potential risk of transmission to humans and other domestic and wild animals is unknown. The data presented here suggest that spotted deer (<i>A. axis</i>) may be a novel host for zoonotic HEV strains.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 4","pages":"281 - 291"},"PeriodicalIF":3.4,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10467717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First Detection and Characterization of Hepatitis E Virus in Sewage Samples in Cameroon 喀麦隆污水样本中首次检测和鉴定戊型肝炎病毒

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-08-09 DOI: 10.1007/s12560-023-09562-3

Modiyinji Abdou Fatawou, Monamele Gwladys Chavely, Moumbeket Yifomnjou Moise Henri, Kamga Njile Daniel, Endegue Zanga Marie Claire, Njouom Richard

{"title":"First Detection and Characterization of Hepatitis E Virus in Sewage Samples in Cameroon","authors":"Modiyinji Abdou Fatawou, Monamele Gwladys Chavely, Moumbeket Yifomnjou Moise Henri, Kamga Njile Daniel, Endegue Zanga Marie Claire, Njouom Richard","doi":"10.1007/s12560-023-09562-3","DOIUrl":"10.1007/s12560-023-09562-3","url":null,"abstract":"<div><p>Hepatitis E virus (HEV) represents an important public health concern in many developing countries, including Africa. Transmission of HEV to humans by contaminated drinking water is the most important mode of transmission in low- and middle-income countries. This study aimed to assess the presence of HEV in the environment in Cameroon through molecular analysis of sewage samples. Retrospectively, a total of 157 sewage samples collected between January 2018 and December 2019 were randomly selected and analyzed by molecular techniques to detect and characterize the HEV followed by sequencing and phylogenetic analysis. Three samples (1.9%) collected from North, Far North, and Adamawa regions were positive by real-time reverse transcription polymerization chain reaction. Among these, 2 samples were positive for HEV ribonucleic acid by nested reverse transcription polymerization chain reaction and only one yielded a good sequencing product. Phylogenetic analysis of this unique HEV strain showed that this HEV strain belonged to genotype 3, subtype 3a, and clustered with swine HEV strains from Cameroon, Argentina, and the USA. This study provides preliminary data on the circulation of HEV in wastewater in Cameroon. Further studies will be needed to assess the overall situation in Cameroon.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 3","pages":"255 - 261"},"PeriodicalIF":3.4,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6709846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Hepatitis A RNA, Hepatitis E RNA, Human Adenovirus F DNA, and Norovirus RNA in Fresh and Frozen Berry Products at Point of Retail in Ireland 爱尔兰零售店新鲜和冷冻浆果产品中甲型肝炎RNA、戊型肝炎RNA、人腺病毒F DNA和诺如病毒RNA的检测

IF 3.4 2区农林科学

Food and Environmental Virology Pub Date : 2023-08-01 DOI: 10.1007/s12560-023-09561-4

Charlene Bennett, Kevin Hunt, Francis Butler, Sinead Keaveney, Séamus Fanning, Cillian De Gascun, Suzie Coughlan, Joanne O’Gorman

{"title":"Detection of Hepatitis A RNA, Hepatitis E RNA, Human Adenovirus F DNA, and Norovirus RNA in Fresh and Frozen Berry Products at Point of Retail in Ireland","authors":"Charlene Bennett, Kevin Hunt, Francis Butler, Sinead Keaveney, Séamus Fanning, Cillian De Gascun, Suzie Coughlan, Joanne O’Gorman","doi":"10.1007/s12560-023-09561-4","DOIUrl":"10.1007/s12560-023-09561-4","url":null,"abstract":"<div><p>Soft fruits are at particular risk of contamination with enteric viruses such as Hepatitis A virus (HAV), Hepatitis E Virus (HEV), Norovirus (NoV), Human Adenovirus (HAdV) and Sapovirus (SaV). The aim of this study was to investigate, for the first time, the presence of these biological agents in ready to eat (RTE) berries at point of retail in Ireland. A sampling strategy was designed in which RTE fresh and frozen strawberries and raspberries were purchased from five retailers between May and October 2018. Reverse Transcriptase Polymerase Chain Reaction (RT-qPCR) assays for HEV RNA, Nov RNA, SaV RNA, and human Adenovirus species F DNA (HAdV-F) were performed on 239 samples (25g portions). Viral nucleic acid was present in 6.7% (<i>n</i> = 16) of samples tested as follows: HAV RNA (<i>n</i> = 5), HAdV-F DNA (<i>n</i> = 5), HEV RNA (<i>n</i> = 3) and NoV GII RNA (<i>n </i>= 3). Sapovirus RNA was not detected in any product. No significant differences were found between berry type, fresh/frozen status, or supermarket source. This study suggests a risk that exists across all retail outlets however only low levels of nucleic acid ranging from 0 to 16 genome copies/g were present. Although these findings may reflect non-viable/non-infectious virus the continued provision of risk mitigation advice to consumers is warranted and further work is required to ensure control measures to reduce contamination are implemented and enforced.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 3","pages":"246 - 254"},"PeriodicalIF":3.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-023-09561-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6709847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1