Food and Environmental Virology最新文献

筛选
英文 中文
Surfactant Treatment for Efficient Gene Detection of Enteric Viruses and Indicators in Surface Water Concentrated by Ultrafiltration 表面活性剂处理对超滤浓缩地表水中肠道病毒及相关指标的高效基因检测
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2023-01-02 DOI: 10.1007/s12560-022-09543-y
{"title":"Surfactant Treatment for Efficient Gene Detection of Enteric Viruses and Indicators in Surface Water Concentrated by Ultrafiltration","authors":"Akihiko Hata,&nbsp;Yuno Meuchi,&nbsp;Miaomiao Liu,&nbsp;Shotaro Torii,&nbsp;Hiroyuki Katayama","doi":"10.1007/s12560-022-09543-y","DOIUrl":"10.1007/s12560-022-09543-y","url":null,"abstract":"<div><p>The hollow fiber ultrafiltration (HFUF)-based microbial concentration method is widely applied for monitoring pathogenic viruses and microbial indicators in environmental water samples. However, the HFUF-based method can co-concentrate substances that interfere with downstream molecular processes—nucleic acid extraction, reverse transcription (RT), and PCR. These inhibitory substances are assumed to be hydrophobic and, therefore, expected to be excluded by a simple surfactant treatment before the silica membrane-based RNA extraction process. In this study, the efficacy and limitations of the sodium deoxycholate (SD) treatment were assessed by quantifying a process control and indigenous viruses using 42 surface water samples concentrated with HFUF. With some exceptions, which tended to be seen in samples with high turbidity (&gt; 4.0 NTU), virus recovery by the ultrafiltration method was sufficiently high (&gt; 10%). RNA extraction-RT-quantitative PCR (RT-qPCR) efficiency of the process control was insufficient (10%) for 30 of the 42 HFUF concentrates without any pretreatments, but it was markedly improved for 21 of the 30 inhibitory concentrates by the SD treatment. Detection rates of indigenous viruses were also improved and no substantial loss of viral RNA was observed. The SD treatment was particularly effective in mitigating RT-qPCR inhibition, although it was not effective in improving RNA extraction efficiency. The methodology is simple and easily applied. These findings indicate that SD treatment can be a good alternative to sample dilution, which is widely applied to mitigate the effect of RT-qPCR inhibition, and can be compatible with other countermeasures.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 1","pages":"8 - 20"},"PeriodicalIF":3.4,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4083795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Performance of Manufacturer Cleaning Recommendations Applied to 3D Food Ink Capsules for the Control of a Human Norovirus Surrogate 制造商清洁建议应用于控制人类诺如病毒替代物的3D食品油墨胶囊的性能
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2022-11-11 DOI: 10.1007/s12560-022-09539-8
{"title":"Performance of Manufacturer Cleaning Recommendations Applied to 3D Food Ink Capsules for the Control of a Human Norovirus Surrogate","authors":"Allyson N. Hamilton,&nbsp;Kristen E. Gibson","doi":"10.1007/s12560-022-09539-8","DOIUrl":"10.1007/s12560-022-09539-8","url":null,"abstract":"<div><p>With the widespread availability of 3D food printing systems for purchase, users can customize their food in new ways. Manufacturer recommendations for cleaning these machines remain untested with regard to the prevention of foodborne pathogen transmission. This study aimed to determine if manufacturer cleaning recommendations for food ink capsules utilized in 3D food printers are adequate to control human norovirus (HuNoV)<i>.</i> A HuNoV surrogate, Tulane virus (TuV; ~ 6 log<sub>10</sub> PFU/mL), was inoculated onto the interior surface of stainless steel food ink capsules. Capsules were either unsoiled or soiled with one of the following: butter, protein powder solution, powdered sugar solution, or a mixture containing all three food components. The capsules were allowed to dry and then one of three hygienic protocols was applied: manual washing (MW), a dishwasher speed cycle (DSC), or a dishwasher heavy cycle (DHC). The interaction effect between DSC and pure butter was a significant predictor of log reduction (<i>P</i> = 0.0067), with the pure butter and DSC combination achieving an estimated mean log reduction of 4.83 (95% CI 4.13, 5.59). The DSC was the least effective method of cleaning when compared with MW and the DHC. The 3-way interaction effects between wash type, soil, and capsule position were a significant predictor of log reduction (<i>P</i> = 0.00341). Capsules with butter in the DSC achieved an estimated mean log reduction of 2.81 (95% CI 2.80, 2.83) for the front-most position versus 6.35 (95% CI 6.33, 6.37) for the back-most position. Soil matrix, cleaning protocol, and capsule position all significantly impact capsule cleanability and potential food safety risk. The DHC is recommended for all capsules, and the corners should be avoided when placing capsules into the dishwasher. The current study seeks to provide recommendations for users of additive manufacturing and 3D food printing including consumers, restaurants, industry, and regulatory industries.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 1","pages":"51 - 60"},"PeriodicalIF":3.4,"publicationDate":"2022-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4474357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Bioaccumulation Dynamic by Crassostrea gigas Oysters of Viruses That Are Proposed as Surrogates for Enteric Virus Contamination in Environmental Samples 被认为是环境样品中肠道病毒污染替代物的长牡蛎对病毒的生物积累动态
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2022-10-26 DOI: 10.1007/s12560-022-09538-9
{"title":"Bioaccumulation Dynamic by Crassostrea gigas Oysters of Viruses That Are Proposed as Surrogates for Enteric Virus Contamination in Environmental Samples","authors":"Vilaine Corrêa da Silva,&nbsp;Mariana Elois,&nbsp;Beatriz Pereira Savi,&nbsp;Marília Miotto,&nbsp;Juliano De Dea Lindner,&nbsp;Gislaine Fongaro,&nbsp;Doris Sobral Marques Souza","doi":"10.1007/s12560-022-09538-9","DOIUrl":"10.1007/s12560-022-09538-9","url":null,"abstract":"<div><p>Oysters are filter-feeders and retain sewage-derived pathogens in their organs or tissues. Since most enteric viruses involved in outbreaks cannot grow in cell culture, studies using viral surrogate models are essential. Some species are proposed as surrogates for enteric viruses in environmental samples, including in bivalve mollusk samples, such as murine norovirus type 1 (MNV-1) and somatic (as φX) or F-specific coliphages (as MS2) bacteriophages. This study evaluated the tissue distribution of viral surrogates for enteric virus contamination after their bioaccumulation by <i>Crassostrea gigas</i>. Oyster tissues were analyzed for the distribution of viral surrogates (MNV-1, φX-174, and MS2) in digestive tissue (DT), gills (GL), and mantle (MT) after 4, 6, and 24 h of experimental bioaccumulation. MNV-1 had higher counts at 6 h in DT (1.2 × 10<sup>3</sup> PFU/g), followed by GL and MT (9.5 × 10<sup>2</sup> and 3.8 × 10<sup>2</sup> PFU/g, respectively). The bacteriophage φX-174 had a higher concentration in the MT at 4 and 6 h (3.0 × 10<sup>2</sup> PFU/g, in both) and MS2 in the GL after 24 h (2.2 × 10<sup>2</sup> PFU/g). The bioaccumulation pattern of MNV-1 by oysters was similar to the other enteric viruses (more in DT), while that of phages followed distinct patterns from these. Since the MNV-1 is bioaccumulated by <i>C. gigas</i> and is adapted to grow in cell culture, it is an important tool for bioaccumulation and viral inactivation tests in oysters. Although bacteriophage bioaccumulation was not similar to enteric viruses, they can be indicated for viral bioaccumulation analysis, analyzing MT and GL, since they do not bioaccumulate in DT.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 1","pages":"1 - 7"},"PeriodicalIF":3.4,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5023489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
One-Year Surveillance of SARS-CoV-2 and Rotavirus in Water Matrices from a Hot Spring Area 某温泉地区水基质中SARS-CoV-2和轮状病毒1年监测
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2022-10-01 DOI: 10.1007/s12560-022-09537-w
{"title":"One-Year Surveillance of SARS-CoV-2 and Rotavirus in Water Matrices from a Hot Spring Area","authors":"M. Salvo,&nbsp;J. Azambuya,&nbsp;N. Baccardatz,&nbsp;A. Moriondo,&nbsp;R. Blanco,&nbsp;M. Martinez,&nbsp;M. Direnna,&nbsp;G. Bertolini,&nbsp;P. Gamazo,&nbsp;R. Colina,&nbsp;E. Alvareda,&nbsp;M. Victoria","doi":"10.1007/s12560-022-09537-w","DOIUrl":"10.1007/s12560-022-09537-w","url":null,"abstract":"<div><p>The pandemic of Coronavirus Disease 2019 (COVID-19) caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is still impacting not only on human health but also all economic activities, especially in those related to tourism. In this study, in order to characterize the presence of SARS-CoV-2 in a hot spring park in Uruguay, swimming pools water, wastewater, and surface water from this area were analyzed by quantitative PCR. Wastewater from Salto city located next to the hydrothermal spring area was also evaluated as well as the presence of Rotavirus (RV). Overall, SARS-CoV-2 was detected in 13% (13/102) of the analyzed samples. Moreover, this virus was not detected in any of the samples from the swimming pools water and was present in 18% (3/17) of wastewater samples from the hotels area showing the same trend between the titer of SARS-CoV-2 and the number of infected people in Salto city. SARS-CoV-2 was also detected in wastewater samples (32% (11/34)) from Salto city, detecting the first positive sample when 105 persons were positive for SARS-CoV-2. Rotavirus was detected only in 10% (2/24) of the wastewater samples analyzed in months when partial lockdown measures were taken, however, this virus was detected in nearly all wastewater samples analyzed when social distancing measures and partial lockdown were relaxed. Wastewater results confirmed the advantages of using the detection and quantification of viruses in this matrix in order to evaluate the presence of these viruses in the population, highlighting the usefulness of this approach to define and apply social distancing. This study suggests that waters from swimming pools are not a source of infection for SARS-CoV-2, although more studies are needed including infectivity assays in order to confirm this statement.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"14 4","pages":"401 - 409"},"PeriodicalIF":3.4,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-022-09537-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4057395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Microgreen Variety Impacts Leaf Surface Persistence of a Human Norovirus Surrogate 微绿变种对人类诺如病毒替代物叶片表面持久性的影响
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2022-09-23 DOI: 10.1007/s12560-022-09536-x
{"title":"Microgreen Variety Impacts Leaf Surface Persistence of a Human Norovirus Surrogate","authors":"Wenjun Deng,&nbsp;Kristen E. Gibson","doi":"10.1007/s12560-022-09536-x","DOIUrl":"10.1007/s12560-022-09536-x","url":null,"abstract":"<div><p>Human norovirus (HuNoV) is a pathogenic agent that is frequently associated with foodborne disease outbreaks linked to fresh produce. Within microgreen production systems, understanding of virus transmission routes and persistence is limited. To investigate virus persistence on microgreen leaf surfaces, this study mimicked virus contaminations caused during microgreen handling by farm workers or during overhead irrigation with contaminated water. Specifically, approximately 5 log PFU of Tulane virus (TV)—a HuNoV surrogate—was inoculated on sunflower (SF) and pea shoot (PS) microgreen leaves at 7-day age. The virus reduction on SF was significantly higher than PS (p &lt; 0.05). On day 10, total TV reduction for SF and PS were 3.70 ± 0.10 and 2.52 ± 0.30 log PFU/plant, respectively. Under the environmental scanning electron microscope (ESEM) observation, the leaf surfaces of SF were visually smoother than PS, while their specific effect on virus persistence were not further characterized. Overall, this study revealed that TV persistence on microgreen leaves was plant variety dependent. In addition, this study provided a preliminary estimation on the risk of HuNoV contamination in a microgreen production system which will aim the future development of prevention and control measures.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 1","pages":"82 - 88"},"PeriodicalIF":3.4,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4921323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Extraction Methods for the Detection of Tick-Borne Encephalitis Virus RNA in Goat Raw Milk and Cream Cheese 山羊原料奶和奶油奶酪中蜱传脑炎病毒RNA提取方法的比较
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2022-09-20 DOI: 10.1007/s12560-022-09535-y
{"title":"Comparison of Extraction Methods for the Detection of Tick-Borne Encephalitis Virus RNA in Goat Raw Milk and Cream Cheese","authors":"Irene Müller,&nbsp;Nadine Althof,&nbsp;Bernd Hoffmann,&nbsp;Christine Klaus,&nbsp;Katja Schilling-Loeffler,&nbsp;Alexander Falkenhagen,&nbsp;Reimar Johne","doi":"10.1007/s12560-022-09535-y","DOIUrl":"10.1007/s12560-022-09535-y","url":null,"abstract":"<div><p>Infection with the tick-borne encephalitis virus (TBEV) can cause meningitis, meningoencephalitis and myelitis in humans. TBEV is an enveloped RNA virus of the family <i>Flaviviridae</i>, which is mostly transmitted via tick bites. However, transmission by consumption of virus-contaminated goat raw milk and goat raw milk products has also been described. Only a few methods have been reported for the detection of TBEV in food so far. Here, we compare different virus extraction methods for goat raw milk and goat raw milk cream cheese and subsequent detection of TBEV-RNA by RT-qPCR. Langat virus (LGTV), a naturally attenuated TBEV strain, was used for artificial contamination experiments. Mengovirus and the human coronavirus 229E were compared to assess their suitability to serve as internal process controls. Out of three tested extraction protocols for raw milk, sample centrifugation followed by direct RNA extraction from the aqueous interphase yielded the best results, with a recovery rate (RR) of 31.8 ± 4.9% for LGTV and a detection limit of 6.7 × 10<sup>3</sup> LGTV genome copies/ml. Out of two methods for cream cheese, treatment of the samples with TRI Reagent® and chloroform prior to RNA extraction showed the best RR of 4.7 ± 1.6% for LGTV and a detection limit of 9.4 × 10<sup>4</sup> LGTV genome copies/g. RRs of Mengovirus and LGTV were similar for both methods; therefore, Mengovirus is suggested as internal process control virus. The developed methods may be useful for screening or surveillance studies, as well as in outbreak investigations.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"15 1","pages":"32 - 42"},"PeriodicalIF":3.4,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-022-09535-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4808214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Hepatitis E Virus in Water Environments: A Systematic Review and Meta-analysis 水环境中的戊型肝炎病毒:系统综述和荟萃分析
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2022-08-29 DOI: 10.1007/s12560-022-09530-3
{"title":"Hepatitis E Virus in Water Environments: A Systematic Review and Meta-analysis","authors":"G. R. Takuissu,&nbsp;S. Kenmoe,&nbsp;L. Ndip,&nbsp;J. T. Ebogo-Belobo,&nbsp;C. Kengne-Ndé,&nbsp;D. S. Mbaga,&nbsp;A. Bowo-Ngandji,&nbsp;M. G. Oyono,&nbsp;R. Kenfack-Momo,&nbsp;S. Tchatchouang,&nbsp;J. Kenfack-Zanguim,&nbsp;R. Lontuo Fogang,&nbsp;E. Zeuko’o Menkem,&nbsp;G. I. Kame-Ngasse,&nbsp;J. N. Magoudjou-Pekam,&nbsp;S. Nkie Esemu,&nbsp;C. Veneri,&nbsp;P. Mancini,&nbsp;G. Bonanno Ferraro,&nbsp;M. Iaconelli,&nbsp;E. Suffredini,&nbsp;G. La Rosa","doi":"10.1007/s12560-022-09530-3","DOIUrl":"10.1007/s12560-022-09530-3","url":null,"abstract":"<div><p>Hepatitis E virus (HEV) is responsible for acute hepatitis in humans, through foodborne, zoonotic, and waterborne transmission routes. This study aimed to assess the prevalence of HEV in water matrices. Six categories were defined: untreated and treated wastewater, surface water (river, lake, and seawater), drinking water, groundwater, and other water environments (irrigation water, grey water, reservoir water, flood water, and effluent of pig slaughterhouse). We searched PubMed, Web of Science, Global Index Medicus, and Excerpta Medica Database. Study selection and data extraction were performed by at least two independent investigators. Heterogeneity (<i>I</i><sup>2</sup>) was assessed using the <i>χ</i><sup>2</sup> test on the Cochran <i>Q</i> statistic and <i>H</i> parameter. Sources of heterogeneity were explored by subgroup analysis. This study is registered with PROSPERO, number CRD42021289116. We included 87 prevalence studies from 58 papers, 66.4% of which performed in Europe. The overall prevalence of HEV in water was 9.8% (95% CI 6.4–13.7). The prevalence was higher in untreated wastewater (15.1%) and lower in treated wastewater (3.8%) and in drinking water (4.7%). In surface water, prevalence was 7.4%, and in groundwater, the percentage of positive samples, from only one study available, was 8.3%. Overall, only 36.8% of the studies reported the genotype of HEV, with genotype 3 (HEV-3) prevalent (168 samples), followed by HEV-1 (148 sample), and HEV-4 (2 samples). High-income countries were the most represented with 59/87 studies (67.8%), while only 3/87 (3.5%) of the studies were performed in low-income countries. The overall prevalence obtained of this study was generally higher in industrialized countries. Risk of bias was low in 14.9% of the studies and moderate in 85.1%. The results of this review showed the occurrence of HEV in different waters environments also in industrialized countries with sanitation and safe water supplies. While HEV transmission to humans through water has been widely demonstrated in developing countries, it is an issue still pending in industrialized countries. Better knowledge on the source of pollution, occurrence, survival in water, and removal by water treatment is needed to unravel this transmission path.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"14 3","pages":"223 - 235"},"PeriodicalIF":3.4,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-022-09530-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5105329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Interactions Between Infectious Foodborne Viruses and Bacterial Biofilms Formed on Different Food Contact Surfaces 传染性食源性病毒与不同食物接触表面形成的细菌生物膜的相互作用
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2022-08-27 DOI: 10.1007/s12560-022-09534-z
{"title":"Interactions Between Infectious Foodborne Viruses and Bacterial Biofilms Formed on Different Food Contact Surfaces","authors":"Marie-Josée Gagné,&nbsp;Tony Savard,&nbsp;Julie Brassard","doi":"10.1007/s12560-022-09534-z","DOIUrl":"10.1007/s12560-022-09534-z","url":null,"abstract":"<div><p>Bacterial biofilms contribute to contamination, spoilage, persistence, and hygiene failure in the food industry, but relatively little is known about the behavior of foodborne viruses evolving in the complex communities that make up biofilm. The aim of this study was to evaluate the association between enteric viruses and biofilms on food contact surfaces. Formed biofilms of mono- and multispecies cultures were prepared on glass, stainless steel, and polystyrene coupons and 10<sup>5</sup> pfu/ml of murine norovirus, rotavirus, and hepatitis A virus were added and incubated for 15 min, 90 min, and 24 h. The data obtained clearly demonstrate that the presence of biofilms generally influences the adhesion of enteric viruses to different surfaces. Many significant increases in attachment rates were observed, particularly with rotavirus whose rate of viral infectious particles increased 7000 times in the presence of <i>Pseudomonas fluorescens</i> on polystyrene after 24 h of incubation and with hepatitis A virus, which seems to have an affinity for the biofilms formed by lactic acid bacteria. Murine norovirus seems to be the least influenced by the presence of biofilms with few significant increases. However, the different factors surrounding this association are unknown and seem to vary according to the viruses, the environmental conditions, and the composition of the biofilm.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"14 3","pages":"267 - 279"},"PeriodicalIF":3.4,"publicationDate":"2022-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-022-09534-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5032051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Evaluation of Methods and Processes for Robust Monitoring of SARS-CoV-2 in Wastewater 废水中SARS-CoV-2稳健性监测方法和流程评价
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2022-08-23 DOI: 10.1007/s12560-022-09533-0
{"title":"Evaluation of Methods and Processes for Robust Monitoring of SARS-CoV-2 in Wastewater","authors":"Olivera Maksimovic Carvalho Ferreira,&nbsp;Živa Lengar,&nbsp;Zala Kogej,&nbsp;Katarina Bačnik,&nbsp;Irena Bajde,&nbsp;Mojca Milavec,&nbsp;Anže Županič,&nbsp;Nataša Mehle,&nbsp;Denis Kutnjak,&nbsp;Maja Ravnikar,&nbsp;Ion Gutierrez-Aguirre","doi":"10.1007/s12560-022-09533-0","DOIUrl":"10.1007/s12560-022-09533-0","url":null,"abstract":"<div><p>The SARS-CoV-2 pandemic has accelerated the development of virus concentration and molecular-based virus detection methods, monitoring systems and overall approach to epidemiology. Early into the pandemic, wastewater-based epidemiology started to be employed as a tool for tracking the virus transmission dynamics in a given area. The complexity of wastewater coupled with a lack of standardized methods led us to evaluate each step of the analysis individually and see which approach gave the most robust results for SARS-CoV-2 monitoring in wastewater. In this article, we present a step-by-step, retrospective view on the method development and implementation for the case of a pilot monitoring performed in Slovenia. We specifically address points regarding the thermal stability of the samples during storage, screening for the appropriate sample concentration and RNA extraction procedures and real-time PCR assay selection. Here, we show that the temperature and duration of the storage of the wastewater sample can have a varying impact on the detection depending on the structural form in which the SARS-CoV-2 target is present. We found that concentration and RNA extraction using Centricon filtration units coupled with Qiagen RNA extraction kit or direct RNA capture and extraction using semi-automated kit from Promega give the most optimal results out of the seven methods tested. Lastly, we confirm the use of N1 and N2 assays developed by the CDC (USA) as the best performing assays among four tested in combination with Fast Virus 1-mastermix. Data show a realistic overall process for method implementation as well as provide valuable information in regards to how different approaches in the analysis compare to one another under the specific conditions present in Slovenia during a pilot monitoring running from the beginning of the pandemic.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"14 4","pages":"384 - 400"},"PeriodicalIF":3.4,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-022-09533-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4882739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Combining Community Wastewater Genomic Surveillance with State Clinical Surveillance: A Framework for SARS-CoV-2 Public Health Practice 社区废水基因组监测与国家临床监测相结合:SARS-CoV-2公共卫生实践框架
IF 3.4 2区 农林科学 SCI
Food and Environmental Virology Pub Date : 2022-08-18 DOI: 10.1007/s12560-022-09531-2
{"title":"Combining Community Wastewater Genomic Surveillance with State Clinical Surveillance: A Framework for SARS-CoV-2 Public Health Practice","authors":"Ted Smith,&nbsp;Rochelle H. Holm,&nbsp;Ray Yeager,&nbsp;Joseph B. Moore IV,&nbsp;Eric C. Rouchka,&nbsp;Kevin J. Sokoloski,&nbsp;Erin M. Elliott,&nbsp;Daymond Talley,&nbsp;Vaneet Arora,&nbsp;Sarah Moyer,&nbsp;Aruni Bhatnagar","doi":"10.1007/s12560-022-09531-2","DOIUrl":"10.1007/s12560-022-09531-2","url":null,"abstract":"<div><p>This study aimed to develop a framework for combining community wastewater surveillance with state clinical surveillance for the confirmation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants within the community and to provide recommendations on how to expand on such research and apply the findings in public health responses. Wastewater samples were collected weekly from 17 geographically resolved locations in Louisville/Jefferson County, Kentucky (USA), from February 10 to December 13, 2021. Genomic surveillance and quantitative reverse transcription PCR (RT-qPCR) platforms were used to screen for SARS-CoV-2 in wastewater, and state clinical surveillance was used for confirmation. The study results highlighted an increased epidemiological value of combining community wastewater genomic surveillance and RT-qPCR with conventional case-auditing methods. The spatial scale and temporal frequency of wastewater sampling provided promising sensitivity and specificity for gaining public health screening insights about SARS-CoV-2 emergence, seeding, and spread in communities. Improved national surveillance systems are needed against future pathogens and variants, and wastewater-based genomic surveillance exhibits great potential when coupled with clinical testing. This paper presents evidence that complementary wastewater and clinical testing are cost-effectively enhanced when used in combination, as they provide a strong tool for a joint public health framework. Future pathogens of interest may be examined in either a targeted fashion or using a more global approach where all pathogens are monitored. This study has also provided novel insights developed from evidence-based public health practices.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"14 4","pages":"410 - 416"},"PeriodicalIF":3.4,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-022-09531-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4710239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信