Molecular Characterization of Gastroenteric Viruses in Wastewater from Cities in Uruguay

IF 4.1 2区农林科学 Q2 ENVIRONMENTAL SCIENCES

Food and Environmental Virology Pub Date : 2023-10-24 DOI:10.1007/s12560-023-09567-y

Bortagaray Viviana, Salvo Matias, Mir Daiana, Colina Rodney, Victoria Matias

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Abstract

Group A Rotavirus, Human Astrovirus, and Norovirus (RVA, HAstV, and NoV) are recognized as the major causative agents of acute gastroenteritis in children and adults worldwide. The aim of this study was to determine the prevalence and molecular epidemiology of RVA, HAstV, and NoV in wastewater from three cities in Uruguay. Thirty-six samples from Bella Unión, Salto, and Fray Bentos cities were analyzed using quantitative and qualitative PCR. RVA was the most frequently detected virus (50%), followed by HAstV (39%), NoV GII (36%), and NoV GI (25%). RVA strains were characterized as P[8] and G3 based on the VP4 and VP7 genes, respectively. Among NoV-positive samples, genotypes GI.2, GI.3, GI.5, GI.6, GI.7, GII.2, GII.6, and GII.4 were detected, and only one HAstV genotype (MLB1) was found. Our wastewater-based epidemiological approach provides a snapshot of the overall genetic diversity of these viruses in three cities of the Uruguay River basin during 2017–2018. These findings reinforce the importance of this environmental surveillance tool for monitoring epidemiological trends of enteric viruses circulating in the population, which can be used to guide public health intervention.

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乌拉圭城市污水中胃肠道病毒的分子特征。

A组轮状病毒、人类星形病毒和诺如病毒（RVA、HAstV和NoV）被认为是全球儿童和成人急性肠胃炎的主要病原体。本研究的目的是确定乌拉圭三个城市废水中RVA、HAstV和NoV的流行率和分子流行病学。使用定量和定性PCR分析了来自Bella Unión、Salto和Fray Bentos城市的36个样本。RVA是最常见的病毒（50%），其次是HAstV（39%）、NoV GII（36%）和NoV GI（25%）。RVA菌株分别基于VP4和VP7基因被鉴定为P[8]和G3。在NoV阳性样本中，检测到基因型GI.2、GI.3、GI.5、GI.6、GI.7、GII.2、GII.6和GII.4，仅发现一个HAstV基因型（MLB1）。我们基于废水的流行病学方法提供了2017-2018年乌拉圭河流域三个城市这些病毒总体遗传多样性的快照。这些发现加强了这种环境监测工具对监测人群中肠道病毒流行趋势的重要性，可用于指导公共卫生干预。

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来源期刊

Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.50

自引率

2.90%

发文量

审稿时长

1 months

期刊介绍： Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.