Food and Environmental Virology最新文献

{"title":"Integrated Clinical and Environmental Surveillance Provides the First Evidence of Aichi Virus Circulation in Córdoba, Argentina","authors":"Ariana Mariela Cachi,&nbsp;María De Los Ángeles Marinzalda,&nbsp;Evangelina Peano,&nbsp;Gonzalo Manuel Castro,&nbsp;Paola Sicilia,&nbsp;María Beatriz Isa,&nbsp;Remigio Arteaga,&nbsp;Verónica Emilse Prez,&nbsp;Silvia Nates,&nbsp;Gisela Masachessi","doi":"10.1007/s12560-026-09698-y","DOIUrl":"10.1007/s12560-026-09698-y","url":null,"abstract":"<div><p>Human Aichi virus (AiV-A1), a member of the <i>Kobuvirus</i> genus associated with viral gastroenteritis, remains largely underdiagnosed in Latin America due to the absence of routine testing and limited epidemiological data. This study provides the first molecular evidence of AiV-A1 circulation in Argentina to date, based on the analysis of wastewater and pediatric stool samples collected in Córdoba between 2012 and 2019. A total of 93 untreated wastewater samples from the main wastewater treatment plant and 155 stool samples from children with acute gastroenteritis (AGE) were screened using a validated nested RT-PCR targeting the 3CD region, followed by sequencing and phylogenetic analysis. AiV-A1 was detected in 58% of wastewater samples, demonstrating continuous, year-round circulation without a defined seasonal pattern. Among clinical AGE cases, AiV-A1 was identified in 21.5%, including 10.3% as monoinfections. Inclusion of AiV-A1 in the diagnostic panel within this study, increased the overall viral detection rate from 62% to 69%, suggesting that its incorporation into routine diagnostics could improve the detection of virologically confirmed diarrhea cases. Phylogenetic reconstruction showed that all Argentine strains clustered within genotype B, forming two intragenotypic lineages and exhibiting high nucleotide identity (94.8%–99.6%) between environmental and clinical isolates. Together, these findings provide the first evidence of endemic AiV-A1 circulation in Argentina, highlight its potencial role in pediatric gastroenteritis, and establish a baseline for future regional epidemiological studies while offering relevant insight into the burden, transmission, and molecular epidemiology of AiV-A1 in the country.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Evaluating Nanotrap Microbiome Particles as A Wastewater Viral Concentration Method","authors":"Marlee Shaffer,&nbsp;Devin North,&nbsp;Kyle Bibby","doi":"10.1007/s12560-026-09697-z","DOIUrl":"10.1007/s12560-026-09697-z","url":null,"abstract":"","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13194239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147979972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Detection of Hepatitis E Virus in Wastewater in Costa Rica","authors":"Bradd Mendoza-Guido,&nbsp;Taimy Calvo,&nbsp;Luis Chacón,&nbsp;María Belén Pisano,&nbsp;Gisela Masachessi,&nbsp;Luz Chacón","doi":"10.1007/s12560-026-09696-0","DOIUrl":"10.1007/s12560-026-09696-0","url":null,"abstract":"<div><p>Hepatitis E virus (HEV) is an important cause of acute viral hepatitis worldwide, with both human-restricted (genotypes 1 and 2) and zoonotic lineages (genotypes 3 and 4). However, information regarding HEV circulation in Central America remains limited. In this study, wastewater-based epidemiological surveillance was conducted in San José, Costa Rica, during 2025 to investigate the presence of HEV in the main wastewater treatment plant serving approximately one million inhabitants. A total of 54 influent and effluent samples were analyzed. HEV RNA was not detected by quantitative RT-PCR; however, RT-nested PCR targeting the ORF1 region identified three positive influent samples. Sequencing yielded two high-quality partial ORF1 sequences suitable for phylogenetic analysis. Both Costa Rican sequences clustered together and showed a close relationship to genotype 3 sequences. To our knowledge, this represents the first report of HEV detected in wastewater in Costa Rica and the first scientific report of environmental detection in Central America. These findings highlight the value of wastewater-based surveillance for identifying circulating viral pathogens in regions where routine clinical testing is limited.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transkingdom Interactions Between Viruses and Bacteria: Implications for Wastewater Treatment Efficiency","authors":"Justin C. Greaves,&nbsp;Christopher M. Robinson","doi":"10.1007/s12560-026-09695-1","DOIUrl":"10.1007/s12560-026-09695-1","url":null,"abstract":"<div><p>Human enteric viruses frequently encounter diverse microbial communities in wastewater, where interactions with bacteria and other microorganisms can profoundly influence their environmental persistence and transmission. Increasing evidence demonstrates that bacteria can bind and stabilize enteric viruses, thereby enhancing thermal inactivation and prolonging infectivity. These interactions not only shape viral fate during wastewater treatment but also may affect the sensitivity and interpretation of wastewater-based epidemiology, an essential tool for public health surveillance. Yet significant knowledge gaps remain regarding the molecular mechanisms that govern bacterial-viral interactions and their roles in broader wastewater microbiomes. Together, advancing our understanding of transkingdom interactions in wastewater is essential for improving treatment processes, strengthening environmental surveillance, and reducing the risk of virus transmission through contaminated water systems.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-026-09695-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147938880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How Wastewater Monitoring is Helping to Investigate a Hepatitis A Outbreak Linked to Oysters’ Consumption","authors":"F. S. Le Guyader,&nbsp;J. Figoni,&nbsp;J. Ollivier,&nbsp;A. Chaghouri,&nbsp;S. Parnaudeau,&nbsp;A. Nisavanh,&nbsp;C. Le Mennec,&nbsp;J. Normand,&nbsp;A. Deslandes,&nbsp;M. Martel,&nbsp;S. Erouart,&nbsp;A.-M. Roque-Afonso","doi":"10.1007/s12560-026-09694-2","DOIUrl":"10.1007/s12560-026-09694-2","url":null,"abstract":"<div><p>In April 2024, two cases of hepatitis A (HA) were linked to the consumption of oysters produced in the same region, which triggered a multidisciplinary study. A network was quickly set up in a one-health approach including epidemiological and clinical data, as well as analysis of locally collected shellfish and wastewater samples. By the end of the outbreak, up to 17 HA cases met the case definition: all shared a same genotype IA strain and have eaten oysters. The prompt response enabled the identification of oyster samples positive for the HAV genome at the start of the monitoring period; these oyster samples subsequently tested negative during the following two months of the sampling campaign. Monitoring three nearby wastewater treatment plants revealed that the contamination was limited to a single area, allowing for more effective monitoring of shellfish. None of the identified cases lived in the area served by the treatment plant, and no clinical cases were detected among the local population, either before or after the outbreak. The decline of HAV concentration in sewage signed the end of the outbreak. While predicting contamination of shellfish growing areas is complicated, sewage surveillance can help establishing an alert system to prevent shellfish contamination, which is an important issue for silent diseases.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-026-09694-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147925208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Viral Pollution in Wastewater and Mediterranean Ecosystems","authors":"Pablo Puchades-Colera,&nbsp;Inés Girón-Guzmán,&nbsp;Enric Cuevas-Ferrando,&nbsp;Azahara Díaz-Reolid,&nbsp;Irene Falcó,&nbsp;Rosa Aznar,&nbsp;Marinella Farré,&nbsp;Marta Llorca,&nbsp;Alba Pérez-Cataluña,&nbsp;Gloria Sánchez","doi":"10.1007/s12560-026-09693-3","DOIUrl":"10.1007/s12560-026-09693-3","url":null,"abstract":"<div>\u0000 \u0000 <p>Increasing water scarcity and climate-related extremes are amplifying the need for safe water reuse, however, wastewater treatment plants (WWTPs) may not fully remove viral pathogens, posing potential risks to freshwater environments. This study assessed human enteric and respiratory viruses using (RT)-qPCR, along with faecal indicators using culture-based methods, at WWTPs in two Mediterranean protected areas (Albufera Natural Park and the Ebro River region) across three campaigns. A total of influent (<i>n</i> = 33), reclaimed wastewater (<i>n</i> = 33), biosolids (<i>n</i> = 32), surface water (<i>n</i> = 33), and sediment (<i>n</i> = 29) samples were analysed. Influent wastewater contained a wide range of viral and faecal indicators, with biosolids showing similar levels. Although reclaimed water demonstrated reductions in <i>Escherichia coli</i> and somatic coliphages, these reductions remained below the ≥ 5 and ≥ 6 Log₁₀ threshold required under EU water reuse regulations, respectively. In surface waters, human enteric viruses were detected at low concentrations, with human norovirus genogroup II (HuNoV GII) showing the highest incidence (36.36%). SARS-CoV-2 was detected in 15.15% of samples, while influenza A virus (IAV) was found in only one sample. Sediments yielded viral signals in 10–35% of samples, whereas <i>E. coli</i> and somatic coliphages were detected in 6.9% and 10.3% of samples, respectively. Hepatitis E virus (HEV) genotype 3f exhibited a clear winter trend, whereas faecal indicators were consistently detected across matrices. In contrast, <i>E. coli</i> and somatic coliphages showed no correlation with viral detections. As the first comprehensive survey of viral presence in these Mediterranean systems, this study provides a baseline for monitoring and managing viral contamination risks during and after extreme climate events, which may exacerbate pathogen mobilization and dissemination. Additionally, the detection of zoonotic viruses, including IAV and HEV, highlights the value of wastewater and environmental surveillance (WES) in biodiversity-rich environments.</p>\u0000 </div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-026-09693-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo Assessment of the Potential Protective Effects of Traditional Kumis Against SARS-CoV-2 in a Transgenic Mouse Model","authors":"Bilal Kocaman,&nbsp;Hatice Nur Aydin,&nbsp;Fatih Ramazan Istanbullugil,&nbsp;Mitat Sahin,&nbsp;Irem Abaci Bayraktar,&nbsp;Arzu Tas Ekiz,&nbsp;Ozge Aksoy Yaman,&nbsp;Hivda Ulbegi Polat","doi":"10.1007/s12560-026-09692-4","DOIUrl":"10.1007/s12560-026-09692-4","url":null,"abstract":"<div>\u0000 \u0000 <p>This study explores the antiviral potential of kumis, a traditional fermented mare’s milk beverage, against SARS-CoV-2 using K18-hACE2 transgenic mice. Kumis samples fermented in leather, wood, and plastic containers were tested to assess their immunomodulatory and antiviral effects. Mice were intranasally infected with live SARS-CoV-2 and orally administered kumis daily for five consecutive days, starting one day prior to viral challenge, and monitored for a total of 15 days. Kumis-treated mice exhibited significantly reduced weight loss at specific time points (days 12 and 15 post-infection; <i>p</i> = 0.0310). Survival was numerically higher in kumis-treated groups (Plastic 5/6, Wooden 5/6, Leather 4/6) compared to controls (3/6), but this difference did not reach statistical significance (<i>p</i> = 0.6649). Histopathological and virological analyses supported the protective effects, with the plastic-fermented sample showing the most pronounced benefit. The study also highlights the importance of fermentation environments on the microbial composition and therapeutic efficacy of kumis. Although kumis is not a direct antiviral drug, it can serve as a supportive food that enhances immunity and provides protection during viral illnesses.</p>\u0000 </div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-026-09692-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-prevalence of SARS-CoV-2 and Noroviruses in Wastewater Collection and Treatment Facilities of the Eastern Cape Region, South Africa: An Exacerbated Public and Environmental Health Risk Concern","authors":"Mbasa Dlamini,&nbsp;Luyanda Msolo,&nbsp;Nolonwabo Nontongana,&nbsp;Kingsley Ehi Ebomah,&nbsp;Anthony Ifeanyi Okoh","doi":"10.1007/s12560-026-09688-0","DOIUrl":"10.1007/s12560-026-09688-0","url":null,"abstract":"<div><p>Severe acute respiratory syndrome Coronavirus-2, Norovirus GI and GII have been identified as one of the leading primary agents of lethal diseases such as COVID-19 and acute gastroenteritis, causing critical problems in public health, particularly in children, the elderly, and individuals with weakened immune systems. These pathogens contribute to substantial morbidity, mortality and pose a serious threat to human health, imposing a tremendous economic burden globally. Hence, this study sought to profile the co-occurrence of SARS-CoV-2 and Noroviruses in wastewater collection and treatment facilities within Buffalo City and Amathole District regions, Eastern Cape Province, South Africa. Raw wastewater samples were collected from the wastewater treatment facilities on a weekly basis for a 6-month sampling regime using the grab sampling technique. Total Ribonucleic acids (RNA) were extracted and purified using the commercial Total RNA extraction kits and the extracted RNA sample were further profiled for the presence of SARS-CoV-2 RNA and Noroviruses GI and GII using RT-qPCR. A total of 213 samples were screened using RT-qPCR. SARS-CoV-2 was detected in 146 (69%) samples, Norovirus GI in 58 (27%), Norovirus GII in 25 (12%) and 25 (12%) samples showed co-infection with both GI and GII norovirus targets. The RNA concentration of the extracted samples from wastewater treatment facilities in this study ranged between 102 ng/µl and 12,828 ng/µl. It was observed that the SARS-CoV-2 GC/mL range from 5.22 × 10² to an extreme 2.5650 × 10⁴ GC/mL. The study findings demonstrate a high prevalence of SARS-CoV-2 RNA in wastewater, affirming the utility of wastewater surveillance as an early warning system for potential outbreaks at community levels. Furthermore, the study showed the co-occurrence of SARS-CoV-2 alongside Norovirus GI and GII in wastewater milieu, highlighting an exacerbated potential risk of co-infection and a major threat to public health and environmental safety.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-026-09688-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral Contaminants in a Philippine Wastewater Treatment Plant: Quantification and Treatment Reduction","authors":"Jessamine Gail M. Inson,&nbsp;Niva Sthapit,&nbsp;Bikash Malla,&nbsp;Shad Natthew S. Arce,&nbsp;Ma. Luisa D. Enriquez,&nbsp;Zeba F. Alam,&nbsp;Eiji Haramoto","doi":"10.1007/s12560-026-09691-5","DOIUrl":"10.1007/s12560-026-09691-5","url":null,"abstract":"<div>\u0000 \u0000 <p>In the Philippines, most of the population relies on decentralized wastewater systems, particularly septic tanks, and the rest on centralized wastewater systems. However, the performance of wastewater treatment systems in the Philippines in reducing viral contaminants remains to be characterized; thus, in this study, the occurrence and reduction of viruses in wastewater before and after treatment were assessed at a wastewater treatment plant (WWTP) that utilizes biological treatment technologies in the Philippines. Influent (<i>n =</i> 18) and effluent (<i>n =</i> 18) samples collected from WWTP from April to August 2024 were centrifuged, followed by viral nucleic acid extraction. Cross-assembly phage (crAssphage) was quantified through quantitative polymerase chain reaction (qPCR), and pepper mild mottle virus (PMMoV), influenza A virus (Inf-A), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and norovirus genogroups I (NoV-GI) and II (NoV-GII) were quantified by reverse transcription-qPCR. All viral targets were quantified in wastewater before and after treatment, except for Inf-A and SARS-CoV-2, which were absent in all effluent samples. The reduction in viral loads in wastewater after treatment was determined, with crAssphage, PMMoV, NoV-GI, and NoV-GII showing high log₁₀ reduction values (LRVs) of 4.35, 4.28, 4.56, and 4.00, respectively, while their lowest LRVs were 2.17, 0.38, 0.72, and − 0.99, respectively. The persistence of crAssphage and PMMoV in wastewater samples and their significant positive associations with NoV-GI and NoV-GII suggest their potential application as indicators of enteric viruses in Philippine wastewater. These findings highlight the importance of viruses in characterizing treatment plant reduction performance and water and wastewater quality in the Philippines.</p>\u0000 </div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-026-09691-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Evaluation of Pepper Mild Mottle Virus as an Indicator in Water Quality Monitoring and Human Fecal Contamination in Swedish Waters","authors":"Marianela Patzi Churqui,&nbsp;Fredy Saguti,&nbsp;Timur Tunovic,&nbsp;Ambjörn Kärmander,&nbsp;Martin Lagging,&nbsp;Kristina Nyström,&nbsp;Hao Wang","doi":"10.1007/s12560-026-09690-6","DOIUrl":"10.1007/s12560-026-09690-6","url":null,"abstract":"<div><p>Pepper mild mottle virus (PMMoV) has emerged as a promising viral indicator for human fecal contamination and evaluating viral removal efficiency in water treatment systems. However, few studies have examined this potential from a genetic perspective over extended timescales. This study investigated PMMoV presence and genetic diversity across multiple water environments in Sweden, including wastewater, lake water, and raw water used for drinking water production, collected between 2016 and 2024. PMMoV was consistently detected in both wastewater and lake water, showing relatively stable concentrations in wastewater between years, and with levels typically 4–7 log₁₀ higher than in lake water. Comparative genotyping of the coat protein (CP) and replication-associated (RP) regions for wastewater samples showed no significant differences. We successfully sequenced 67 isolates from wastewater, 16 from lake water, and 2 from source water at the CP region. Sequence analysis revealed extremely high nucleotide identities (&gt; 96%) with minimal divergence between samples collected six years apart or from different water types. Phylogenetic comparisons with global reference sequences confirmed high genetic conservation worldwide. These findings demonstrate the remarkable genetic stability of PMMoV over time and geography, supporting its reliability as a viral process indicator for water treatment systems. However, the potential for PMMoV to originate from non-human sources suggests it should not be used as a standalone marker for human-specific fecal contamination. Overall, this study highlights the global genetic conservation of PMMoV and its utility for long-term environmental surveillance and water quality assessment.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147715575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}