Food and Environmental Virology最新文献

{"title":"Wastewater-Based Epidemiology of Hepatitis A in Mexico: Temporal Association with Hospital Admissions and Clinical-Environmental Phylogenetic Relatedness","authors":"Mayra Colunga-Saucedo,&nbsp;César David Barcenas-Rivera,&nbsp;Pedro Gerardo Hernández-Sánchez,&nbsp;Martín Magaña-Aquino,&nbsp;María Susana Juárez-Tobías,&nbsp;Juan Carlos Muñoz-Escalante,&nbsp;Daniel E. Noyola,&nbsp;Pedro Torres-González","doi":"10.1007/s12560-026-09704-3","DOIUrl":"10.1007/s12560-026-09704-3","url":null,"abstract":"<div><p>Hepatitis A (HepA) is a viral hepatitis caused by the hepatitis A virus (HAV), and currently Mexico is considered to have an intermediate-HAV endemicity. Wastewater-based epidemiology (WBE) is a tool for monitoring infectious diseases but has been poorly evaluated in intermediate-HAV-endemicity settings. From June 2024 to June 2025, 24-hour composite wastewater samples were collected twice a week from three wastewater treatment plants in the metropolitan area of San Luis Potosí, Mexico. Wastewater samples were ultracentrifuged, RNA was extracted, and HAV and crAssphage (for normalization) were detected by qPCR and weighted by the wastewater treatment plant’s inflow. We compared these results with HepA hospital admissions from eight public hospitals in the city during the same period. We compared HAV genotype sequences obtained from wastewater and patients. We processed 244 wastewater samples over 46 weeks, of which 35.1% tested positive for HAV. Seventy-three HepA hospital admissions were reported during this period. The Spearman correlation between HAV viral load and hospital admissions, along with its displacement over 10 weeks, showed the strongest correlation between Lag 4 to Lag 6. We genotyped six HAV strains from wastewater samples and two from HepA patients. Phylogenetic analysis revealed that the patient strains were distinct but identical or closely related to the circulating wastewater strains. In conclusion, WBE HAV surveillance may predict an increase in HepA admissions four to six weeks in advance. Diverse, but closely related HAV strains constantly circulate in intermediate HAV-endemicity settings.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148323757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Food Processing and Preservation on the Survivability of Group A Rotavirus (SA11) in Simulated and Real Food Models","authors":"Mahshid Heydari,&nbsp;Atefeh Kachooei,&nbsp;Reza Mohammadi,&nbsp;Omid Rostami,&nbsp;Mehrnaz Hosseini Tehrani,&nbsp;Milad Rouhi,&nbsp;Angila Ataei-Pirkooh","doi":"10.1007/s12560-026-09703-4","DOIUrl":"10.1007/s12560-026-09703-4","url":null,"abstract":"<div><p>Rotavirus SA11 (RV SA11), a simian strain and member of group A, is widely used for RV research due to its attenuation and similarity to human strains. This study investigated the effects of food acidifying agents and common food processing and preservation conditions on the survivability of RV SA11. Acetic, citric, and lactic buffers (adjusted to pH 4.0) as simulated buffer systems and real food models, including traditional pickle, orange juice, and a fermented yogurt drink, were inoculated with RV SA11 at an initial titer of 7.22 log₁₀ TCID₅₀/ml. Viral titers were determined after exposing samples to refrigeration (4 °C for 2 and 4 days), room temperature (20 °C for 2 and 6 h), heating (90 °C for 10 and 30 min), and microwave treatment (0.5 and 2 min). At 4 °C, a 4.99 log₁₀ TCID₅₀/ml reduction was observed in the lactic acid system after 4 days, while complete inactivation occurred within 2 days in the citric and 4 days in the acetic systems. At 20 °C, viral titers decreased by 4.45, 3.99, and 3.06 log₁₀ TCID₅₀/ml in lactic, citric, and acetic systems, respectively. Heating at 90 °C completely inactivated the virus within 10 min in lactic and 30 min in acetic systems, while microwave exposure for 2 min eliminated infectivity in both acetic and citric buffers. Viral reductions in real foods after 6 h at 20 °C were lower than in simulated buffers (a range of 2.45 to 3.39 log₁₀ TCID₅₀/ml). Overall, these findings show that food composition and processing conditions significantly influence rotavirus persistence and inactivation in food matrices.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Wastewater-Based Surveillance of Tomato Brown Rugose Fruit Virus (ToBRFV) in Korea","authors":"Seoyeon Mo,&nbsp;Youngrae Jo,&nbsp;Ju Hee Kim,&nbsp;Do Gyun Lee","doi":"10.1007/s12560-026-09702-5","DOIUrl":"10.1007/s12560-026-09702-5","url":null,"abstract":"<div><p>Monitoring human fecal contamination in wastewater is crucial for public health and environmental safety. Traditional fecal indicator bacteria (FIB), such as <i>Escherichia coli</i> and <i>Enterococci</i>, lack source specificity between human and non-human sources. Tomato brown rugose fruit virus (ToBRFV) has emerged as a promising viral indicator, characterized by high environmental stability, specificity for human feces, and widespread occurrence in wastewater systems. This study evaluates ToBRFV as a novel RNA-based viral indicator for wastewater surveillance in Korea. Wastewater samples were collected from six wastewater treatment plants (WWTPs) in Incheon between July 2024 and April 2025. The results showed that ToBRFV was detected in all influent samples (240/240), with concentrations ranging from 7.42 to 9.91 log₁₀ gene copies/L and low coefficients of variation (3.36%-6.39%). The observed concentrations were significantly higher than those reported from other regions in previous studies, confirming high abundance and stable detection of ToBRFV. This study represents the first quantitative assessment of ToBRFV in Korean wastewater using digital PCR. These findings suggest that ToBRFV has potential as a reliable human fecal contamination indicator and normalization marker for wastewater-based epidemiology (WBE) applications in Korean wastewater systems.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148261749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Fit-for-Purpose, High Quality Proficiency Samples for Interlaboratory Evaluation of RT-PCR Detection of HPAI H5N1 in Milk","authors":"Emily L. Smith,&nbsp;Yuhan Jin,&nbsp;Megan R. Miller,&nbsp;Kirstin Frost,&nbsp;Jodie Ulaszek,&nbsp;Sarah M. Nemser,&nbsp;Laura B. Goodman,&nbsp;Steffen Uhlig,&nbsp;Anja Schlierf,&nbsp;Karina Hettwer,&nbsp;Matthew Kmet,&nbsp;Andriy Tkachenko,&nbsp;Olgica Ceric,&nbsp;Gregory H. Tyson,&nbsp;Ravinder Reddy","doi":"10.1007/s12560-026-09699-x","DOIUrl":"10.1007/s12560-026-09699-x","url":null,"abstract":"<div><p>The increased demand for highly pathogenic avian influenza (HPAI) testing in milk highlights the critical need for well-characterized samples that enable meaningful evaluation of current detection methods. In this study, we focus on the development and rigorous characterization of artificially contaminated spiked milk samples, forming the foundation for reliable assessment of assay performance. By using reverse transcriptase digital PCR (RT-dPCR), we obtained precise genome copy (GC) measurements of our virus stock and of spiked samples, producing accurate quantification even at low viral loads. In parallel, the U.S. Department of Agriculture National Veterinary Services Laboratories’ (USDA-NVSL’s) influenza A matrix gene (IAV M) real-time reverse transcriptase (rRT-PCR) assay was employed as the main detection assay, serving as a tool to validate the sample preparation methodology. The goal of this work is to show that robust, reproducible, and analytically traceable samples can be generated to support HPAI method evaluation in proficiency exercises (PEs) distributed nationwide. Low between-sample standard deviations, ranging from 0 to 0.10 rRT-PCR Ct values for replicate samples, fall below the theoretical threshold indicative of heterogeneity, along with RT-dPCR determinations of sample GCs affirm the homogeneity of the developed samples. Parallel analysis of corresponding milk-based and phosphate buffered saline (PBS)-based samples showed no indication of a matrix effect. No significant shifts of Ct value or of measured GCs were observed over time, proving samples to be stable for at least 28 days when stored at -80 °C. The defined assay sensitivity, expressed as the level of detection 50% (LOD_50%), of the USDA-NVSL’s IAV M rRT-PCR assay was determined to be 3.2 GCs/rRT-PCR reaction for milk samples and 5.5 GCs/rRT-PCR reaction for PBS samples, but this difference is not significant. This work sets the basis for determining laboratory competency, providing confidence in HPAI testing results for ongoing and future surveillance.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-026-09699-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148196681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic Modelling of the Decay of Respiratory Syncytial Viruses (RSV) Facilitated a Robust Wastewater-Based Surveillance for RSV","authors":"Yuqing Long,&nbsp;Jiayi Liu,&nbsp;Xiuyuan Shi,&nbsp;Xueling Yang,&nbsp;Yongyan Long,&nbsp;Jinzhou Xiao,&nbsp;Xinxin Han,&nbsp;Songzhe Fu","doi":"10.1007/s12560-026-09701-6","DOIUrl":"10.1007/s12560-026-09701-6","url":null,"abstract":"<div><p>Wastewater-based epidemiology (WBE) is a promising tool for monitoring respiratory pathogens, yet the rapid in-sewer decay of viruses limits its application in low-incidence settings. This study investigates the decay rates of respiratory syncytial virus (RSV) in wastewater under various conditions to enhance the utility of WBE for forecasting RSV outbreaks. Laboratory-scale recirculating sewer systems were employed to simulate in-sewer decay of RSV at different temperatures, organic concentrations, and total suspended solids (TSS) levels. A first-order decay model revealed a significant temperature dependence, with the decay rate increasing approximately 30-fold from 4 to 35 °C. Conversely, higher TSS concentrations provided substantial protection to RSV particles, extending the T₉₀ by up to sevenfold, whereas SCOD exhibited minimal impact. Based on these findings, a multiple linear regression model was established to identify key predictors. This study underscores the importance of understanding RSV decay kinetics for accurate WBE and highlights the value of kinetic modelling in correcting in-sewer viral loss for optimized wastewater surveillance.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148173207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Viruses of Public Health Importance in Wastewater Samples Using Conventional PCR Techniques and a Targeted Enrichment Whole Genome Sequencing Panel","authors":"Gonzalo M. Castro,&nbsp;Florencia Mallou,&nbsp;Gisella Masachessi,&nbsp;María C. Frutos,&nbsp;Verónica E. Prez,&nbsp;Tomás Poklepovich,&nbsp;Silvia V. Nates,&nbsp;María B. Pisano,&nbsp;Viviana E. Ré","doi":"10.1007/s12560-026-09700-7","DOIUrl":"10.1007/s12560-026-09700-7","url":null,"abstract":"<div><p>Wastewater-based epidemiology is an effective approach for monitoring viruses of public health relevance in the community. PCR/qPCR are widely used for targeted detection, while NGS with targeted enrichment panels has emerged as a complementary strategy for broader viral detection and genomic characterization. This study comparatively evaluated PCR/qPCR and a targeted enrichment whole-genome sequencing Viral Surveillance Panel (VSP, Illumina) for virus detection in wastewater. Fifty-two wastewater samples collected between 2017 and 2023 from a wastewater treatment plant in Córdoba, Argentina, were concentrated by PEG precipitation and pooled by season and year, reaching 14 pools. Each pool was analyzed in parallel by PCR/qPCR for eight human viruses of public health importance and by the VSP, targeting 66 viral species, sequenced on a NovaSeq 6000 platform, and analyzed with the DRAGEN pipeline. Detection frequencies for each virus using PCR/qPCR and VSP were Rotavirus A 100%/14.3%; Norovirus 100%/14.3%; Aichivirus 50%/42.9%; SARS-CoV-2 14.3%/0%; HAV 42.9%/0%; HEV 14.3%/0%; JCPyV 35.7%/85.7%; BKPyV 28.6%/71.4%, respectively. In addition, VSP detected the genomes of Astrovirus (71.4%), Salivirus (21.4%), Coxsackievirus A19 (14.3%), Rotavirus C (14.3%), and Merkel Cell virus (7.1%), and enable the recovery of 16 near complete genomes of Aichivirus, JCPyV, BKPyV, Salivirus and Astrovirus. PCR provided the sensitivity and speed necessary for viral screening of the main human pathogens of public health concern, while the VSP generated valuable genomic information on understudied viruses. According to these results, PCR/qPCR panels would represent a more practical, sensitive and cost-effective alternative for wastewater key public health viruses’ surveillance purposes in our region.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148100802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Clinical and Environmental Surveillance Provides the First Evidence of Aichi Virus Circulation in Córdoba, Argentina","authors":"Ariana Mariela Cachi,&nbsp;María De Los Ángeles Marinzalda,&nbsp;Evangelina Peano,&nbsp;Gonzalo Manuel Castro,&nbsp;Paola Sicilia,&nbsp;María Beatriz Isa,&nbsp;Remigio Arteaga,&nbsp;Verónica Emilse Prez,&nbsp;Silvia Nates,&nbsp;Gisela Masachessi","doi":"10.1007/s12560-026-09698-y","DOIUrl":"10.1007/s12560-026-09698-y","url":null,"abstract":"<div><p>Human Aichi virus (AiV-A1), a member of the <i>Kobuvirus</i> genus associated with viral gastroenteritis, remains largely underdiagnosed in Latin America due to the absence of routine testing and limited epidemiological data. This study provides the first molecular evidence of AiV-A1 circulation in Argentina to date, based on the analysis of wastewater and pediatric stool samples collected in Córdoba between 2012 and 2019. A total of 93 untreated wastewater samples from the main wastewater treatment plant and 155 stool samples from children with acute gastroenteritis (AGE) were screened using a validated nested RT-PCR targeting the 3CD region, followed by sequencing and phylogenetic analysis. AiV-A1 was detected in 58% of wastewater samples, demonstrating continuous, year-round circulation without a defined seasonal pattern. Among clinical AGE cases, AiV-A1 was identified in 21.5%, including 10.3% as monoinfections. Inclusion of AiV-A1 in the diagnostic panel within this study, increased the overall viral detection rate from 62% to 69%, suggesting that its incorporation into routine diagnostics could improve the detection of virologically confirmed diarrhea cases. Phylogenetic reconstruction showed that all Argentine strains clustered within genotype B, forming two intragenotypic lineages and exhibiting high nucleotide identity (94.8%–99.6%) between environmental and clinical isolates. Together, these findings provide the first evidence of endemic AiV-A1 circulation in Argentina, highlight its potencial role in pediatric gastroenteritis, and establish a baseline for future regional epidemiological studies while offering relevant insight into the burden, transmission, and molecular epidemiology of AiV-A1 in the country.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Evaluating Nanotrap Microbiome Particles as A Wastewater Viral Concentration Method","authors":"Marlee Shaffer,&nbsp;Devin North,&nbsp;Kyle Bibby","doi":"10.1007/s12560-026-09697-z","DOIUrl":"10.1007/s12560-026-09697-z","url":null,"abstract":"","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13194239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147979972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Detection of Hepatitis E Virus in Wastewater in Costa Rica","authors":"Bradd Mendoza-Guido,&nbsp;Taimy Calvo,&nbsp;Luis Chacón,&nbsp;María Belén Pisano,&nbsp;Gisela Masachessi,&nbsp;Luz Chacón","doi":"10.1007/s12560-026-09696-0","DOIUrl":"10.1007/s12560-026-09696-0","url":null,"abstract":"<div><p>Hepatitis E virus (HEV) is an important cause of acute viral hepatitis worldwide, with both human-restricted (genotypes 1 and 2) and zoonotic lineages (genotypes 3 and 4). However, information regarding HEV circulation in Central America remains limited. In this study, wastewater-based epidemiological surveillance was conducted in San José, Costa Rica, during 2025 to investigate the presence of HEV in the main wastewater treatment plant serving approximately one million inhabitants. A total of 54 influent and effluent samples were analyzed. HEV RNA was not detected by quantitative RT-PCR; however, RT-nested PCR targeting the ORF1 region identified three positive influent samples. Sequencing yielded two high-quality partial ORF1 sequences suitable for phylogenetic analysis. Both Costa Rican sequences clustered together and showed a close relationship to genotype 3 sequences. To our knowledge, this represents the first report of HEV detected in wastewater in Costa Rica and the first scientific report of environmental detection in Central America. These findings highlight the value of wastewater-based surveillance for identifying circulating viral pathogens in regions where routine clinical testing is limited.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transkingdom Interactions Between Viruses and Bacteria: Implications for Wastewater Treatment Efficiency","authors":"Justin C. Greaves,&nbsp;Christopher M. Robinson","doi":"10.1007/s12560-026-09695-1","DOIUrl":"10.1007/s12560-026-09695-1","url":null,"abstract":"<div><p>Human enteric viruses frequently encounter diverse microbial communities in wastewater, where interactions with bacteria and other microorganisms can profoundly influence their environmental persistence and transmission. Increasing evidence demonstrates that bacteria can bind and stabilize enteric viruses, thereby enhancing thermal inactivation and prolonging infectivity. These interactions not only shape viral fate during wastewater treatment but also may affect the sensitivity and interpretation of wastewater-based epidemiology, an essential tool for public health surveillance. Yet significant knowledge gaps remain regarding the molecular mechanisms that govern bacterial-viral interactions and their roles in broader wastewater microbiomes. Together, advancing our understanding of transkingdom interactions in wastewater is essential for improving treatment processes, strengthening environmental surveillance, and reducing the risk of virus transmission through contaminated water systems.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"18 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-026-09695-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147938880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}