Food and Environmental Virology最新文献

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Wastewater Surveillance to Estimate and Characterize Hepatitis E Virus Circulation 污水监测评估和表征戊型肝炎病毒循环
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-05-21 DOI: 10.1007/s12560-025-09644-4
C. Dimeglio, O. Schlosser, S. Laperche, C. De Smet, S. Demmou, J. Latour, N. Jeanne, M. Tribout, N. Bleuez, J. Figoni, F. Abravanel, S. Lhomme, J. Izopet
{"title":"Wastewater Surveillance to Estimate and Characterize Hepatitis E Virus Circulation","authors":"C. Dimeglio,&nbsp;O. Schlosser,&nbsp;S. Laperche,&nbsp;C. De Smet,&nbsp;S. Demmou,&nbsp;J. Latour,&nbsp;N. Jeanne,&nbsp;M. Tribout,&nbsp;N. Bleuez,&nbsp;J. Figoni,&nbsp;F. Abravanel,&nbsp;S. Lhomme,&nbsp;J. Izopet","doi":"10.1007/s12560-025-09644-4","DOIUrl":"10.1007/s12560-025-09644-4","url":null,"abstract":"<div><p>Hepatitis E virus (HEV) is a cause of enterically transmitted hepatitis around the world. Because of the high frequency of asymptomatic infections, the magnitude of HEV infection is underestimated. Wastewater monitoring could be useful to improve our knowledge on HEV epidemiology. In this study, we analyzed the capacity of wastewater surveillance to give an insight into the circulation and the diversity of HEV in two French cities. HEV RNA was detected and quantified by digital PCR in 115 untreated composite wastewater samples collected weekly at the inlet of wastewater treatment plants (WWTPs), 58 at Toulouse WWTP and 57 at Dunkerque WWTP. Plasma HEV RNA in blood donors was detected by a commercial assay (Roche Cobas) over the same period in the same area. HEV diversity was analyzed using long-read single-molecule real-time sequencing (Pacific Biosciences). HEV RNA was detected in 88% and 95% wastewater samples collected at Toulouse (Occitanie region, Southern France) and Dunkerque (Hauts-de-France region, Northern France) WWTPs, respectively. HEV RNA concentration ranged between 4.1 and 5.7 log copies/L and was almost similar between the two sites. A long <i>orf2</i> fragment of HEV genome (1030 nucleotides) was obtained and sequenced in 45% and 70% of positive HEV RNA wastewater samples collected at Toulouse site and Dunkerque site, respectively. Out of 31 strains identified in Toulouse wastewater, 24 were HEV-3c (77%), 6 were HEV-3f (19%), and 1 was HEV-3h (3%). Out of 55 strains identified in Dunkerque, 30 were HEV-3c (55%) and 25 were HEV-3f (45%). All HEV RNA-positive samples from blood donors that could be genotyped during the study period contained HEV-3. Subtype distribution in 51 blood donors living in Toulouse did not differ from that in Toulouse wastewater. The HEV-3 subtype distribution in 51 Hauts-de-France region blood donors and in Dunkerque wastewater were different, but the predominant subtype was the same (HEV-3c). Lastly, we explored the link between the measurement of viral loads in wastewater and the extent of infection in the served population. Although a good correlation between the peaks of positive HEV RNA estimated in wastewater samples and that observed in blood donors was observed with a lag of + 3 weeks for Toulouse, the correlation was weaker for Dunkerque. Wastewater surveillance system applied locally could be very useful for assessing the HEV infection status of a population.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"17 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-025-09644-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antiviral Activity of Allyl Isothiocyanate Against Infectious Viruses 异硫氰酸烯丙酯对感染性病毒的抗病毒活性
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-05-20 DOI: 10.1007/s12560-025-09643-5
Irene Falcó, Gloria Sánchez
{"title":"Antiviral Activity of Allyl Isothiocyanate Against Infectious Viruses","authors":"Irene Falcó,&nbsp;Gloria Sánchez","doi":"10.1007/s12560-025-09643-5","DOIUrl":"10.1007/s12560-025-09643-5","url":null,"abstract":"<div><p>The effects of allyl isothiocyanate (AITC) on enteric viruses, specifically hepatitis A virus (HAV) and murine norovirus (MNV) as a norovirus surrogate, were evaluated at different concentrations, temperatures, and exposure time. AITC at 0.1 and 0.5% was mixed with each virus and incubated at 10, 25, and 37 °C for 2 h or overnight. AITC demonstrated a concentration-, temperature-, and time-dependent antiviral effect, with the lowest concentration resulting in a modest decrease in viral titer. However, at the highest concentration and 37 °C during overnight incubation, reductions of 3.75 log TCID50/mL for MNV and below the limit of detection for HAV were reported. Additionally, efficacy of AITC was evaluated on human norovirus (HuNoV) GI suspensions using an in situ capture quantitative reverse transcription polymerase chain reaction (RT-qPCR) method. The results indicated that HuNoVs are susceptible to AITC at 37 °C, which partially inhibits the interaction between the viral capsid and its receptor. Furthermore, AITC was tested as a natural disinfectant for produce with treatment times of 15 and 30 min, with no statistically significant changes in viral titers. Although further optimization of AITC application is required, these findings suggest that AITC has potential as a tool to reduce enteric virus contamination on food and food-contact surfaces.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"17 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-025-09643-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Presence of Potentially Infectious Human Enteric Viruses and Antibiotic Resistance Genes in Mussels from the Campania Region, Italy: Implications for Consumer’s Safety 意大利坎帕尼亚地区贻贝中存在潜在传染性人类肠道病毒和抗生素耐药基因:对消费者安全的影响
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-05-15 DOI: 10.1007/s12560-025-09635-5
Iolanda Venuti, Enric Cuevas-Ferrando, Irene Falcó, Inés Girón-Guzmán, Marina Ceruso, Tiziana Pepe, Gloria Sánchez
{"title":"Presence of Potentially Infectious Human Enteric Viruses and Antibiotic Resistance Genes in Mussels from the Campania Region, Italy: Implications for Consumer’s Safety","authors":"Iolanda Venuti,&nbsp;Enric Cuevas-Ferrando,&nbsp;Irene Falcó,&nbsp;Inés Girón-Guzmán,&nbsp;Marina Ceruso,&nbsp;Tiziana Pepe,&nbsp;Gloria Sánchez","doi":"10.1007/s12560-025-09635-5","DOIUrl":"10.1007/s12560-025-09635-5","url":null,"abstract":"<div><p>This study presents a comprehensive assessment of viral contamination and antibiotic resistance genes (ARGs) presence in mussels (<i>Mytilus galloprovincialis</i>) (<i>n</i> = 60) collected from retail stores in the Campania region (Italy). High prevalence of human noroviruses (HuNoV) genogroup I (GI) (77%) and genogroup II (GII) (40%), rotaviruses (RV) (60%), and astroviruses (HAstV) (25%) was found, with average levels of 4.34, 5.09, 5.05, and 4.00 Log genome copies (GC)/g, respectively. All samples tested negative for hepatitis A and E viruses. Viral faecal contamination indicators, including somatic coliphages (88%, 3.62 mean Log plaque forming units (PFU)/100 g) and crAssphage (50%, 3.72 mean Log GC/g), showed strong correlations (<i>ρ</i> &gt; 0.65, p-value &lt; 0.05) with HuNoV GII, HAstV, and RV concentrations in mussels. The study also investigated the presence of respiratory viruses, with all samples testing negative for SARS-CoV-2, respiratory syncytial virus, and influenza A virus.</p><p>Furthermore, a capsid-integrity RT-qPCR assay was applied to selected positive samples, confirming the presence of potentially infectious viruses and underscoring the associated risks to consumers.</p><p>Additionally, ARGs were detected by qPCR, targeting beta-lactams, quinolones, and chloramphenicol resistance genes in both the total and the bacteriophage fractions of selected samples.</p><p>Overall, this study emphasizes the importance of continued surveillance and strategic interventions to mitigate public health risks associated with the consumption of contaminated bivalve molluscan shellfish (BMS), which may imply the dissemination of infectious enteric viruses and ARGs within communities.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"17 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-025-09635-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wastewater-Based Epidemiology for Analysis of Human Papillomavirus Infections in a Uruguayan Urban Area 乌拉圭城市地区人类乳头瘤病毒感染的废水流行病学分析
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-04-29 DOI: 10.1007/s12560-025-09642-6
C. Fernandez-Sabatella, G. Corrales, L. Monzon, E. Andrade, N. Sica, E. Cardozo, N. Baccardatz, J. Jacques, S. Castro, M. Castells, M. Victoria, R. Colina
{"title":"Wastewater-Based Epidemiology for Analysis of Human Papillomavirus Infections in a Uruguayan Urban Area","authors":"C. Fernandez-Sabatella,&nbsp;G. Corrales,&nbsp;L. Monzon,&nbsp;E. Andrade,&nbsp;N. Sica,&nbsp;E. Cardozo,&nbsp;N. Baccardatz,&nbsp;J. Jacques,&nbsp;S. Castro,&nbsp;M. Castells,&nbsp;M. Victoria,&nbsp;R. Colina","doi":"10.1007/s12560-025-09642-6","DOIUrl":"10.1007/s12560-025-09642-6","url":null,"abstract":"<div><p>Human papillomavirus (HPV) is primarily transmitted through sexual contact and is classified into high- and low-risk genotypes based on their association with cancer development. High-risk (HR) genotypes, such as 16 and 18, among others, have been identified as responsible for the development of cervical cancer while low-risk (LR) genotypes, such as 6 and 11, among others, cause anogenital warts. The aim of this study was to determine the presence of HPV genotypes in wastewater from the wastewater treatment plant of the city of Salto, Uruguay in order to analyze the circulating HPV strains in their population. These samples were subjected to qualitative PCR analysis, and genotypes were identified through sequencing of the DNA products. HPV 6, 16, 31, 66, 81, 84, and 145 were frequently detected in wastewater and HPV 6 and 16 were the prevalent in cytological samples. A great diversity of genotypes was evident in the wastewater of the city. The approach of wastewater-based epidemiology as a representation of the circulating HPV genotypes in the population is adequate and an important tool for molecular epidemiologic studies mainly in developing countries such as Uruguay where information concerning genotypes circulation is scarce. </p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"17 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Persistence of Tick-Borne Encephalitis Virus in Goat and Cow Milks Under Different Storage Conditions and Following Thermal Inactivation 不同储存条件和热灭活后羊奶和牛奶中蜱传脑炎病毒的持久性
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-04-18 DOI: 10.1007/s12560-025-09641-7
Laure Mathews-Martin, Camille V. Migné, Teheipuaura Mariteragi-Helle, Lisa Fourniol, Raphaëlle Metras, Laure Bournez, Marine Dumarest, Catherine Hennechart-Collette, Sylvie Perelle, Sandra Martin-Latil, Gaëlle Gonzalez
{"title":"Persistence of Tick-Borne Encephalitis Virus in Goat and Cow Milks Under Different Storage Conditions and Following Thermal Inactivation","authors":"Laure Mathews-Martin,&nbsp;Camille V. Migné,&nbsp;Teheipuaura Mariteragi-Helle,&nbsp;Lisa Fourniol,&nbsp;Raphaëlle Metras,&nbsp;Laure Bournez,&nbsp;Marine Dumarest,&nbsp;Catherine Hennechart-Collette,&nbsp;Sylvie Perelle,&nbsp;Sandra Martin-Latil,&nbsp;Gaëlle Gonzalez","doi":"10.1007/s12560-025-09641-7","DOIUrl":"10.1007/s12560-025-09641-7","url":null,"abstract":"<div><p>Tick-borne encephalitis virus (TBEV) is a neuroinvasive arbovirus that is primarily transmitted to humans through the bites of <i>Ixodes ricinus</i> ticks. Consumption of unpasteurised milk and dairy products from infected ruminants can also cause infection in humans. In the majority of food-borne TBE (FB-TBE) cases, goat milk and/or cheese has been identified as the source of infection. The aim of the present study was to analyse the persistence of the infectious strain TBEV_Ain_2020 virus in spiked goat and cow raw milks under different storage conditions, and following pasteurisations performed at 63 °C/30 min or 72 °C/15 s. The total genome of TBEV was stable up to 48 h in goat and cow’s milks at 4 °C and 21 °C. In contrast, the viral titre was significantly lower in goat milk from T + 2 h post-contamination up to 17 h compared to culture cell medium and cow milk at 4 °C. At 21 °C, viral titres were lower than in DMEM in both milks up to T + 12 h. Thermal inactivations were effective in goat milk, but were not sufficient to eliminate all infective virus particles in cow milk. These unexpected findings highlighted that pasteurisation processes should be adapted to the species of origin of the milk and to the initial viral load to ensure food safety.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"17 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-025-09641-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Iron on Viral Infections 铁对病毒感染的影响
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-04-10 DOI: 10.1007/s12560-025-09638-2
Yasmin Ferreira Souza Hoffmann Jempierre, Catielen Paula Pavi, Iara Zanella Guterres, Izabella Thaís da Silva, Gislaine Fongaro
{"title":"Effect of Iron on Viral Infections","authors":"Yasmin Ferreira Souza Hoffmann Jempierre,&nbsp;Catielen Paula Pavi,&nbsp;Iara Zanella Guterres,&nbsp;Izabella Thaís da Silva,&nbsp;Gislaine Fongaro","doi":"10.1007/s12560-025-09638-2","DOIUrl":"10.1007/s12560-025-09638-2","url":null,"abstract":"<div><p>Iron is a cofactor in various biological processes, primarily obtained through dietary intake and also through oral or intravenous supplementation. Elevated iron levels are associated with increased production of reactive oxygen species, causing cellular damage. Additionally, iron influences the body’s response to infections and participates in the synthesis of genetic material and cellular functions. Therefore, this review aims to explore the complex interplay between iron homeostasis and viral infections, analyzing how iron availability affects viral replication, possible mutations, and pathogenesis. The interaction between viruses and iron, although less explored in the literature, indicates the influence of host iron bioavailability on parasite–host interactions. Furthermore, iron absorption is regulated by hepcidin, a peptide hormone produced by the liver, which reduces blood iron levels by inhibiting ferroportin function. Iron is important in viral growth and activities, potentially promoting replication, possible mutations, and increased virulence as seen in some studies with respiratory, enteric, and other viral models. Thus, iron chelators can be a promising preventive therapeutic strategy to limit iron availability and thereby reduce viral infectivity.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"17 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vitro Efficacy of Foam Hand Sanitizers Against Enveloped and Non-Enveloped Viruses 泡沫洗手液对包膜和非包膜病毒的体外效果观察
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-04-03 DOI: 10.1007/s12560-025-09640-8
Francis Torko, Kristen E. Gibson
{"title":"In Vitro Efficacy of Foam Hand Sanitizers Against Enveloped and Non-Enveloped Viruses","authors":"Francis Torko,&nbsp;Kristen E. Gibson","doi":"10.1007/s12560-025-09640-8","DOIUrl":"10.1007/s12560-025-09640-8","url":null,"abstract":"<div><p>Enveloped and non-enveloped virus transmission can occur via person-to-person contact and potentially through contaminated surfaces with human hands. Establishing the efficacy of hand sanitizers, including gel and foam formats, is crucial in reducing the transmission of viruses of human health concern, yet foam hand sanitizers are generally underexplored despite being widely used. Following American Society for Testing and Materials (ASTM) E1052-20, the efficacy of foam-based hand sanitizers—one non-alcohol-based hand sanitizer and four alcohol-based hand sanitizers with benzalkonium chloride and ethanol as active ingredients, respectively—were explored using bacteriophage phi6 (Φ6) as a surrogate for enveloped viruses and bacteriophage MS2 (<i>Emesvirus zinderi</i>) and Tulane virus (TuV) as surrogates for non-enveloped viruses. Significant differences in log reduction were observed among viruses (<i>P</i> ≤ 0.05). After a 10 s exposure, a 5.23 ± 1.64 log reduction was observed for Φ6 while MS2 remained resistant (0.04 ± 0.08 log<sub>10</sub> reduction). Conversely, significant log reductions (<i>P</i> ≤ 0.05) were observed for TuV across all foam-based hand sanitizer products ranging from 0.07 ± 0.1 to 1.09 ± 0.22. An exposure time of 10 s (i.e., the typical rubbing time in real-world scenarios following hand sanitizer application) is likely sufficient for enveloped virus inactivation based on the inactivation of bacteriophage Φ6 by the tested commercially available products. However, longer exposure times or different hand sanitizer formulations may be required to achieve similar log reductions against non-enveloped viruses such as human norovirus based on the surrogates (MS2, TuV) tested.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"17 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-025-09640-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Occurrence and Molecular Characterization of Human Astrovirus and Hepatitis A Virus in Bivalve Mollusks Marketed in Tourist Cities in Rio de Janeiro, Brazil 巴西里约热内卢旅游城市销售的双壳类软体动物中人类星状病毒和甲型肝炎病毒的发生及分子特征
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-04-02 DOI: 10.1007/s12560-025-09639-1
Natália Lourenço dos Santos, Fernanda Marcicano Burlandy, Andreza Soriano Figueiredo, Bruna Figueiredo Lopes, Livia Melo Villar, Adriana Gonçalves Maranhão, Caroline Roberta Soares Salgado, Marcelo Luiz Lima Brandão, Marize Pereira Miagostovich, José Paulo Gagliardi Leite, Carina Pacheco Cantelli
{"title":"Occurrence and Molecular Characterization of Human Astrovirus and Hepatitis A Virus in Bivalve Mollusks Marketed in Tourist Cities in Rio de Janeiro, Brazil","authors":"Natália Lourenço dos Santos,&nbsp;Fernanda Marcicano Burlandy,&nbsp;Andreza Soriano Figueiredo,&nbsp;Bruna Figueiredo Lopes,&nbsp;Livia Melo Villar,&nbsp;Adriana Gonçalves Maranhão,&nbsp;Caroline Roberta Soares Salgado,&nbsp;Marcelo Luiz Lima Brandão,&nbsp;Marize Pereira Miagostovich,&nbsp;José Paulo Gagliardi Leite,&nbsp;Carina Pacheco Cantelli","doi":"10.1007/s12560-025-09639-1","DOIUrl":"10.1007/s12560-025-09639-1","url":null,"abstract":"<div><p>This study investigated the prevalence and genetic diversity of human astrovirus (HAstV), hepatitis A virus (HAV), and hepatitis E virus (HEV) in bivalve mollusks (mussels and oysters) marketed in three tourist cities in the State of Rio de Janeiro, Brazil, from January to December 2022. One hundred and thirty-four samples were processed according to the ISO 15216-1:2017 (Microbiology of food a chain—horizontal method for determination of hepatitis A virus and norovirus in food using real-time RTPCR—Part 1: method for quantification, vol 2017. International Organization for Standardization, Geneva, pp 1–48, 2017), and viral screening was performed by the TaqMan real-time RT-qPCR. HAstV RNA was detected in 13.9% (10/72) of the oyster samples and 14.5% (9/62) of the mussel samples. HAV RNA was detected in 8.1% (5/62) of the mussels, while HEV RNA was not detected in any of the analyzed bivalves. The molecular characterization revealed that HAstV strains detected in live oysters belonged to both classical (HAstV-1) and non-classical (MLB-1) genotypes. The HAV-IA genotype was detected in mussel samples and segregated into two subclusters. This study reports the presence of HAstV and HAV in oysters and mussels marketed in Brazil for the first time. The findings indicate local water contamination in the bivalve sampling areas, highlighting the importance of environmental monitoring and surveillance improvements, particularly in shellfish production areas.</p></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"17 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Navigating Uncertainties in RT-qPCR and Infectivity Assessment of Norovirus RT-qPCR的不确定性导航及诺如病毒的感染性评估
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-03-08 DOI: 10.1007/s12560-024-09632-0
Razieh Sadat Mirmahdi, Samantha L. Dicker, Nuradeen Garba Yusuf, Naim Montazeri
{"title":"Navigating Uncertainties in RT-qPCR and Infectivity Assessment of Norovirus","authors":"Razieh Sadat Mirmahdi,&nbsp;Samantha L. Dicker,&nbsp;Nuradeen Garba Yusuf,&nbsp;Naim Montazeri","doi":"10.1007/s12560-024-09632-0","DOIUrl":"10.1007/s12560-024-09632-0","url":null,"abstract":"<div><p>Human norovirus (HuNoV) is the primary cause of gastroenteritis globally. Due to the lack of a reliable cultivation system, RT-qPCR is a gold standard technique for the detection and quantification of HuNoV. However, the inability of PCR to differentiate between infectious from non-infectious particles remains a significant limitation. This study aims to address this limitation by exploring the relationship between culture-based (plaque assay and TCID<sub>50</sub>) and non-culture-based (RT-qPCR) methods for HuNoV quantification, using Tulane virus as a cultivable surrogate. The ultracentrifuge-purified Tulane virus at 6.7 log<sub>10</sub> PFU/ml or 5.8 log<sub>10</sub> TCID<sub>50</sub>/ml in Tris–EDTA buffer (pH 7.2), was serially diluted and subjected to RNA extraction, with or without RNase pretreatment, followed by quantification with RT-qPCR. Further physical characterization of the virus stock was performed with dynamic light scattering and transmission electron microscopy. A strong correlation (Pearson’s Correlation Coefficient of 0.99) was observed between log<sub>10</sub> genome copies (GC) and log<sub>10</sub> plaque forming units (PFU) per PCR reaction for both RNase-pretreated and unpretreated samples. Beta distributions indicated a similar median GC:PFU ratio of ca. 3.7 log<sub>10</sub> for both RNase-pretreated and unpretreated samples. The high GC:PFU ratio may indicate the sensitive nature of RT-qPCR or the presence of intact, non-infectious virus particles. The outcomes of this study will contribute to the more accurate estimation of infectious norovirus particles in food and environmental matrices.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div><div><p>Created in BioRender. Mirmahdi, R. (2024) https://BioRender.com/l49a583</p></div></div></figure></div></div>","PeriodicalId":563,"journal":{"name":"Food and Environmental Virology","volume":"17 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12560-024-09632-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of An Enveloped Virus Surrogate, Bacteriophage Phi6, Recovery from Hands 一种包膜病毒替代物ph6噬菌体的优化
IF 4.1 2区 农林科学
Food and Environmental Virology Pub Date : 2025-03-04 DOI: 10.1007/s12560-025-09637-3
Francis Torko, Kristen E. Gibson
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