Occurrence and Molecular Characterization of Human Astrovirus and Hepatitis A Virus in Bivalve Mollusks Marketed in Tourist Cities in Rio de Janeiro, Brazil

IF 4.1 2区 农林科学 Q2 ENVIRONMENTAL SCIENCES
Natália Lourenço dos Santos, Fernanda Marcicano Burlandy, Andreza Soriano Figueiredo, Bruna Figueiredo Lopes, Livia Melo Villar, Adriana Gonçalves Maranhão, Caroline Roberta Soares Salgado, Marcelo Luiz Lima Brandão, Marize Pereira Miagostovich, José Paulo Gagliardi Leite, Carina Pacheco Cantelli
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引用次数: 0

Abstract

This study investigated the prevalence and genetic diversity of human astrovirus (HAstV), hepatitis A virus (HAV), and hepatitis E virus (HEV) in bivalve mollusks (mussels and oysters) marketed in three tourist cities in the State of Rio de Janeiro, Brazil, from January to December 2022. One hundred and thirty-four samples were processed according to the ISO 15216-1:2017 (Microbiology of food a chain—horizontal method for determination of hepatitis A virus and norovirus in food using real-time RTPCR—Part 1: method for quantification, vol 2017. International Organization for Standardization, Geneva, pp 1–48, 2017), and viral screening was performed by the TaqMan real-time RT-qPCR. HAstV RNA was detected in 13.9% (10/72) of the oyster samples and 14.5% (9/62) of the mussel samples. HAV RNA was detected in 8.1% (5/62) of the mussels, while HEV RNA was not detected in any of the analyzed bivalves. The molecular characterization revealed that HAstV strains detected in live oysters belonged to both classical (HAstV-1) and non-classical (MLB-1) genotypes. The HAV-IA genotype was detected in mussel samples and segregated into two subclusters. This study reports the presence of HAstV and HAV in oysters and mussels marketed in Brazil for the first time. The findings indicate local water contamination in the bivalve sampling areas, highlighting the importance of environmental monitoring and surveillance improvements, particularly in shellfish production areas.

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来源期刊
Food and Environmental Virology
Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY
CiteScore
6.50
自引率
2.90%
发文量
35
审稿时长
1 months
期刊介绍: Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.
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