Genomics DataPub Date : 2017-06-01DOI: 10.1016/j.gdata.2017.02.008
Atul Kumar , D. Jeya Sundara Sharmila , Sachidanand Singh
{"title":"SVMRFE based approach for prediction of most discriminatory gene target for type II diabetes","authors":"Atul Kumar , D. Jeya Sundara Sharmila , Sachidanand Singh","doi":"10.1016/j.gdata.2017.02.008","DOIUrl":"10.1016/j.gdata.2017.02.008","url":null,"abstract":"<div><p>Type II diabetes is a chronic condition that affects the way our body metabolizes sugar. The body's important source of fuel is now becoming a chronic disease all over the world. It is now very necessary to identify the new potential targets for the drugs which not only control the disease but also can treat it. Support vector machines are the classifier which has a potential to make a classification of the discriminatory genes and non-discriminatory genes. SVMRFE a modification of SVM ranks the genes based on their discriminatory power and eliminate the genes which are not involved in causing the disease. A gene regulatory network has been formed with the top ranked coding genes to identify their role in causing diabetes. To further validate the results pathway study was performed to identify the involvement of the coding genes in type II diabetes. The genes obtained from this study showed a significant involvement in causing the disease, which may be used as a potential drug target.</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 28-37"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.02.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34796951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The draft genome sequence of the plant growth promoting rhizospheric bacterium Enterobacter cloacae SBP-8","authors":"Rajnish Prakash Singh, Shrayansh Nalwaya, Prabhat Nath Jha","doi":"10.1016/j.gdata.2017.03.006","DOIUrl":"10.1016/j.gdata.2017.03.006","url":null,"abstract":"<div><p>Here, we report the draft genome sequence and annotation of plant growth promoting rhizobacterium <em>Enterobacter cloacae</em> SBP-8 isolated from the rhizosphere of <em>Sorghum bicolor</em> L. growing in desert region of Rajasthan, India. From the genome sequences, we identified the genes encoding plant growth promoting properties such as 1-aminocyclopropane-1-carboxylate deaminase (<em>AcdS</em>), phosphate solubilisation, siderophore, and IAA (indole acetic acid) production. The genes encoding different functions required for colonization including motility, chemotaxis, adherence, and secretion system (I, II, IV, VI) were also identified. The complete genome sequence of this bacterium consists of one chromosome (48,54,065<!--> <!-->bp) and one plasmid (85,398). The genome sequence of <em>Enterobacter cloacae</em> SBP-8 was deposited in the Genbank with the accession number <span>CP016906</span><svg><path></path></svg> (chromosome) and CP017413 (plasmid).</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 81-83"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.03.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34892891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genomics DataPub Date : 2017-06-01DOI: 10.1016/j.gdata.2017.05.010
Laura J. Grenville-Briggs , Sandeep K. Kushwaha , Michelle R. Cleary , Johanna Witzell , Eugene I. Savenkov , Stephen C. Whisson , Aakash Chawade , Ramesh R. Vetukuri
{"title":"Draft genome of the oomycete pathogen Phytophthora cactorum strain LV007 isolated from European beech (Fagus sylvatica)","authors":"Laura J. Grenville-Briggs , Sandeep K. Kushwaha , Michelle R. Cleary , Johanna Witzell , Eugene I. Savenkov , Stephen C. Whisson , Aakash Chawade , Ramesh R. Vetukuri","doi":"10.1016/j.gdata.2017.05.010","DOIUrl":"10.1016/j.gdata.2017.05.010","url":null,"abstract":"<div><p><em>Phytophthora cactorum</em> is a broad host range phytopathogenic oomycete. <em>P. cactorum</em> strain LV007 was isolated from a diseased European Beech (<em>Fagus sylvatica</em>) in Malmö, Sweden in 2016. The draft genome of <em>P. cactorum</em> strain LV007 is 67.81<!--> <!-->Mb. It contains 15,567 contigs and 21,876 predicted protein-coding genes. As reported for other phytopathogenic Phytophthora species, cytoplasmic effector proteins including RxLR and CRN families were identified. The genome sequence has been deposited at DDBJ/ENA/GenBank under the accession NBIJ00000000. The version described in this paper is version NBIJ01000000.</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 155-156"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.05.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35040864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genomics DataPub Date : 2017-06-01DOI: 10.1016/j.gdata.2017.05.003
Muhammad Mu'izzuddin Zulkapli, Muhammad Aqil Fitri Rosli, Faris Imadi Mohd Salleh, Normah Mohd Noor, Wan Mohd Aizat, Hoe-Han Goh
{"title":"Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants","authors":"Muhammad Mu'izzuddin Zulkapli, Muhammad Aqil Fitri Rosli, Faris Imadi Mohd Salleh, Normah Mohd Noor, Wan Mohd Aizat, Hoe-Han Goh","doi":"10.1016/j.gdata.2017.05.003","DOIUrl":"https://doi.org/10.1016/j.gdata.2017.05.003","url":null,"abstract":"<div><p>Tropical pitcher plants in the species-rich Nepenthaceae family of carnivorous plants possess unique pitcher organs. Hybridisation, natural or artificial, in this family is extensive resulting in pitchers with diverse features. The pitcher functions as a passive insect trap with digestive fluid for nutrient acquisition in nitrogen-poor habitats. This organ shows specialisation according to the dietary habit of different Nepenthes species. In this study, we performed the first single-molecule real-time isoform sequencing (Iso-Seq) analysis of full-length cDNA from <em>Nepenthes ampullaria</em> which can feed on leaf litter, compared to carnivorous <em>Nepenthes rafflesiana</em>, and their carnivorous hybrid <em>Nepenthes</em> <!-->×<!--> <em>hookeriana</em>. This allows the comparison of pitcher transcriptomes from the parents and the hybrid to understand how hybridisation could shape the evolution of dietary habit in Nepenthes. Raw reads have been deposited to SRA database with the accession numbers SRX2692198 (<em>N. ampullaria</em>), SRX2692197 (<em>N. rafflesiana</em>), and SRX2692196 (<em>N.</em> <!-->×<!--> <em>hookeriana</em>).</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 130-131"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92077596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genomics DataPub Date : 2017-06-01DOI: 10.1016/j.gdata.2017.05.004
Diyana Jamaluddin, Normah Mohd Noor, Hoe-Han Goh
{"title":"Transcriptome analysis of Carica papaya embryogenic callus upon De-etiolated 1 (DET1) gene suppression","authors":"Diyana Jamaluddin, Normah Mohd Noor, Hoe-Han Goh","doi":"10.1016/j.gdata.2017.05.004","DOIUrl":"https://doi.org/10.1016/j.gdata.2017.05.004","url":null,"abstract":"<div><p>Papaya is considered to be one of the most nutritional fruits. It is rich in vitamins, carotenoids, flavonoids and other phytonutrient which function as antioxidant in our body <span>[1]</span>. Previous studies revealed that the suppression of a negative regulator gene in photomorphogenesis, De-etiolated 1 (<em>DET</em>1) can improve the phytonutrient in tomato and canola without affecting the fruit quality <span>[2]</span>, <span>[3]</span>. This report contains the experimental data on high-throughput 3′ mRNA sequencing of transformed papaya callus upon <em>DET</em>1 gene suppression.</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 120-121"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.05.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92077597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genomics DataPub Date : 2017-06-01DOI: 10.1016/j.gdata.2017.02.013
Muhammad Afzal , Sulman Shafeeq
{"title":"Impact of aspirin on the transcriptome of Streptococcus pneumoniae D39","authors":"Muhammad Afzal , Sulman Shafeeq","doi":"10.1016/j.gdata.2017.02.013","DOIUrl":"10.1016/j.gdata.2017.02.013","url":null,"abstract":"<div><p>Aspirin or acetylsalicylic acid (ASA) is a medicine used to treat pain, fever, and inflammation. Here, we for the very first time reported the genome-wide transcriptional profiling of aspirin-regulated genes in <em>Streptococcus pneumoniae</em> in the presence of 5<!--> <!-->mM aspirin in chemically-defined medium (CDM) using microarray analysis. Our results showed that expression of several genes was differentially expressed in the presence of aspirin. These genes were further grouped into COG (Clusters of Orthologous Groups) functional categories based on the putative functions of the corresponding proteins. Most of affected genes belong to COG category E (Amino acid transport and metabolism), G (Carbohydrate transport and metabolism), J (Translation, ribosomal structure and biogenesis), and I (Lipid transport and metabolism). Transcriptional profiling data of aspirin-regulated genes was deposited to Gene Expression Omnibus (GEO) database under accession number <span>GSE94514</span><svg><path></path></svg>.</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 38-40"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.02.013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34809366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genomics DataPub Date : 2017-06-01DOI: 10.1016/j.gdata.2017.02.010
Subhadipa Sengupta , Sayak Ganguli , Pankaj K Singh
{"title":"Metagenome analysis of the root endophytic microbial community of Indian rice (O. sativa L.)","authors":"Subhadipa Sengupta , Sayak Ganguli , Pankaj K Singh","doi":"10.1016/j.gdata.2017.02.010","DOIUrl":"10.1016/j.gdata.2017.02.010","url":null,"abstract":"<div><p>This study reports the root endophytic microbial community profile in rice (<em>Oryza sativa</em> L.), the largest food crop of Asia, using 16S rRNA gene amplicon sequencing. Metagenome of OS01 and OS04 consisted of 11,17,900 sequences with 300<!--> <!-->Mbp size and average 55.6% G<!--> <!-->+<!--> <!-->C content. Data of this study are available at NCBI Bioproject (PRJNA360379). The taxonomic analysis of 843 OTU's showed that the sequences belonged to four major phyla revealing dominance of Proteobacteria, Firmicutes, Cyanobacteria and Actinobacteria. Results reveal the dominance of <em>Bacillus</em> as major endophytic genera in rice roots, probably playing a key role in Nitrogen fixation.</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 41-43"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.02.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34809367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genomics DataPub Date : 2017-06-01DOI: 10.1016/j.gdata.2017.02.004
Abhishek Gupta , Avishek Dutta , Jayeeta Sarkar , Dhiraj Paul , Mruganka Kumar Panigrahi , Pinaki Sar
{"title":"Metagenomic exploration of microbial community in mine tailings of Malanjkhand copper project, India","authors":"Abhishek Gupta , Avishek Dutta , Jayeeta Sarkar , Dhiraj Paul , Mruganka Kumar Panigrahi , Pinaki Sar","doi":"10.1016/j.gdata.2017.02.004","DOIUrl":"10.1016/j.gdata.2017.02.004","url":null,"abstract":"<div><p>Mine tailings from copper mines are considered as one of the sources of highly hazardous acid mine drainage (AMD) due to bio-oxidation of its sulfidic constituents. This study was designed to understand microbial community composition and potential for acid generation using samples from mine tailings of Malanjkhand copper project (MCP), India through 16S rRNA gene based amplicon sequencing approach (targeting V4 region). Three tailings samples (T1, T2 and T3) with varied physiochemical properties selected for the study revealed distinct microbial assemblages. Sample (T3) with most extreme nature (pH<!--> <!--><<!--> <!-->2.0) harbored <em>Proteobacteria</em>, <em>Actinobacteria</em>, <em>Chloroflexi</em> while the samples (T1 and T3) with slightly moderate nature (pH<!--> <!--><<!--> <!-->4.0 and ><!--> <!-->3.0) exhibited abundance of <em>Proteobacteria</em>, <em>Fimicutes</em>, <em>Actinobacteria</em> and/or <em>Nitrospirae</em>. Metagenomic sequences are available under the BioProject ID PRJNA361456.</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 11-13"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.02.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34766521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Virulence genome analysis of Pseudomonas aeruginosa VRFPA10 recovered from patient with scleritis","authors":"Nandagopal Murugan , Jambulingam Malathi , Vetrivel Umashankar , Hajib Narahari Rao Madhavan","doi":"10.1016/j.gdata.2017.02.007","DOIUrl":"10.1016/j.gdata.2017.02.007","url":null,"abstract":"<div><p>Infectious keratitis is a major cause of blindness, next to cataract and majority of cases are mainly caused by gram negative bacterium <em>Pseudomonas aeruginosa (P</em>. <em>aeruginosa)</em>. In this study, we investigated a <em>P</em>. <em>aeruginosa</em> VRFPA10 genome which exhibited susceptibility to commonly used drugs <em>in vitro</em> but the patient had poor prognosis due to its hyper virulent nature. Genomic analysis of VRFPA10 deciphered multiple virulence factors and <em>P</em>.<em>aeruginosa</em> Genomic Islands (PAGIs) VRFPA10 genome which correlated with hyper virulence nature of the organism. The genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession numbers LFMZ01000001-LFMZ01000044.</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 1-3"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.02.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44686456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities","authors":"A.A. Pochtovyy , O.Yu. Baranov , I.E. Rubel' , O.A. Razumova , V.E. Padutov , A.V. Khromov , A.V. Makhotenko , A.P. Tkachuk , V.V. Makarov , V.A. Gushchin","doi":"10.1016/j.gdata.2017.03.010","DOIUrl":"https://doi.org/10.1016/j.gdata.2017.03.010","url":null,"abstract":"<div><p>The most common type of coniferous mobile genetic elements are retrotransposons. Despite of their early positive impact on evolution of modern coniferous species they can have a significant negative impact for Forestry and breeding. Breaking genomic structural integrity mobile elements can cause phenotypic defects of plants. In this regard, the study of the diversity of coniferous mobile genetic elements is particularly interesting. In the present paper, we describe mobile genetic elements in dsRNA fraction of <em>Pinus sylvestris</em> having witches broom abnormalities. In result of assembled contigs analysis by RepeatMasker 70 mobile genetic elements were identified. A 68 of that were retroelements. Most of elements represented by <em>Gypsy</em> (16 contigs) and <em>Copia</em> (48 contigs). In 4 cases retroelements specific to <em>Pinus taeda</em> were identified. In most cases fragments of integrase (24), reverse transcriptase (22) and RNaseH (15) were identified. Results of the study may be of interest for coniferous breeding and genetic specialists.</p><p>The raw data of these experiments have been deposited at NCBI under the accession number SAMN06185845.</p></div>","PeriodicalId":56340,"journal":{"name":"Genomics Data","volume":"12 ","pages":"Pages 102-108"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gdata.2017.03.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92030634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}