Metagenome analysis of the root endophytic microbial community of Indian rice (O. sativa L.)

Subhadipa Sengupta , Sayak Ganguli , Pankaj K Singh
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引用次数: 30

Abstract

This study reports the root endophytic microbial community profile in rice (Oryza sativa L.), the largest food crop of Asia, using 16S rRNA gene amplicon sequencing. Metagenome of OS01 and OS04 consisted of 11,17,900 sequences with 300 Mbp size and average 55.6% G + C content. Data of this study are available at NCBI Bioproject (PRJNA360379). The taxonomic analysis of 843 OTU's showed that the sequences belonged to four major phyla revealing dominance of Proteobacteria, Firmicutes, Cyanobacteria and Actinobacteria. Results reveal the dominance of Bacillus as major endophytic genera in rice roots, probably playing a key role in Nitrogen fixation.

Abstract Image

Abstract Image

印度水稻根系内生微生物群落的宏基因组分析
本研究利用16S rRNA基因扩增子测序技术对亚洲最大的粮食作物水稻根系内生微生物群落进行了分析。OS01和OS04的宏基因组包含117,900个序列,大小为300 Mbp,平均G + C含量为55.6%。本研究的数据可在NCBI Bioproject (PRJNA360379)上获得。对843个OTU序列的分类分析表明,这些序列属于4个主要门,显示出变形菌门、厚壁菌门、蓝藻门和放线菌门的优势。结果表明,芽孢杆菌是水稻根系的主要内生菌属,可能在固氮过程中起关键作用。
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