Plants-Basel最新文献

{"title":"Synergistic Interaction Between Endophytic <i>Bacillus pumilus</i> and Indigenous Arbuscular Mycorrhizal Fungi Complex Improves Photosynthetic Activity, Growth, and Yield of <i>Pisum sativum</i>.","authors":"Mounia Akhallaa Youne, Oumnia Akhallaa Youne, Mohammed Bouskout, Yaseen Khan, Hamza Khassali, Sulaiman Shah, Ahmed Sujat, Hassan Alahoui, Mohamed Najib Alfeddy, Bacem Mnasri, Lahcen Ouahmane","doi":"10.3390/plants14131991","DOIUrl":"10.3390/plants14131991","url":null,"abstract":"<p><p>The demand for sustainable agriculture has prompted the exploration of alternative methods to boost crop growth and yield. Microbial biostimulants offer effective solutions to enhance plant performance and reduce reliance on chemical fertilizers. This study investigated the effects of <i>Bacillus pumelo</i> (<i>B. pumilus</i>), applied individually and in combination with a mycorrhizal fungi complex, on the growth, yield, and photosynthetic activity of pea (<i>Pisum sativum</i>). Pea seeds were grown in sterilized soil under four treatment conditions, including a non-inoculated control, inoculation with 2.5 mL of <i>B. pumilus</i> culture per seedling, inoculation with an indigenous mycorrhizal fungal complex, and a combined treatment of <i>B. pumilus</i> and the mycorrhizal complex. The biostimulant treatments significantly influenced all measured photosynthetic and growth parameters. The results showed that <i>B. pumilus</i> substantially promoted pea growth, leading to notable improvements in biomass, plant height, and photosynthetic efficiency. When combined with the mycorrhizal fungi complex, these growth-promoting effects were significantly amplified, resulting in a ~69.7% increase in shoot fresh weight, a ~72.7% rise in root dry weight, and a ~73.6% boost in flower production. Additionally, the chlorophyll content increased by ~180% and photosynthetic yield (F<i>v</i>/F<i>m</i>) improved by ~18.5%. The combined treatment also produced the highest SPAD index value, reflecting a ~57% increase. The synergistic interaction between <i>B. pumilus</i> and mycorrhizal fungi enhances photosynthetic efficiency and overall plant performance. The study highlights the potential of using these microbial inoculants as biostimulants to improve pea cultivation in agroecosystems, offering a sustainable alternative to chemical fertilizers.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12252046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spray-Dried <i>Celtis iguanaea</i> (Jacq.) Planch (Cannabaceae) Extract: Building Evidence for Its Therapeutic Potential in Pain and Inflammation Management.","authors":"Kátia Regina Ribeiro, Rúbia Bellard E Silva, João Paulo Costa Rodrigues, Mairon César Coimbra, Laura Jéssica Pereira, Emmilly de Oliveira Alves, Flávio Martins de Oliveira, Marx Osório Araújo Pereira, Eric de Souza Gil, Carlos Alexandre Carollo, Nadla Soares Cassemiro, Camile Aparecida da Silva, Pablinny Moreira Galdino de Carvalho, Flávia Carmo Horta Pinto, Renan Diniz Ferreira, Zakariyya Muhammad Bello, Edilene Santos Alves de Melo, Marina Andrade Rocha, Ana Gabriela Silva, Rosy Iara Maciel Azambuja Ribeiro, Adriana Cristina Soares, Renê Oliveira do Couto","doi":"10.3390/plants14132008","DOIUrl":"10.3390/plants14132008","url":null,"abstract":"<p><p><i>Celtis iguanaea,</i> widely used in Brazilian folk medicine, is known for its analgesic and anti-inflammatory properties. This study evaluated the <i>in vitro</i> antioxidant capacity and the <i>in vivo</i> antinociceptive and anti-inflammatory mechanisms of the standardized spray-dried <i>Celtis iguanaea</i> hydroethanolic leaf extract (SDCi). Phytochemical analysis showed that SDCi contains 21.78 ± 0.82 mg/g polyphenols, 49.69 ± 0.57 mg/g flavonoids, and 518.81 ± 18.02 mg/g phytosterols. UFLC-DAD-MS identified iridoid glycosides, p-coumaric acid glycosides, flavones, and unsaturated fatty acids. Antioxidant assays revealed an IC₅₀ of 301.6 ± 38.8 µg/mL for DPPH scavenging and an electrochemical index of 6.1 μA/V. <i>In vivo</i>, SDCi (100-1000 mg/kg, p.o) did not impair locomotor function (rotarod test) but significantly reduced acetic acid-induced abdominal writhing and both phases of the formalin test at higher doses (300 and 1000 mg/kg). The antinociceptive effects were independent of α-2 adrenergic receptors. SDCi also increased latency in the hot-plate test and reduced paw edema in the carrageenan model, accompanied by decreased IL-1β and increased IL-10 levels. Histological analysis showed a 50% reduction in inflammatory cell infiltration. These findings support SDCi as an effective anti-inflammatory and antinociceptive phytopharmaceutical intermediate, with potential applications in managing pain and inflammation.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12251947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Mechanisms Underlying Citral-Induced Oxidative Stress and Its Contribution to Antifungal Efficacy on <i>Magnaporthe oryzae</i> Through a Multi-Omics Approach.","authors":"Yonghui Huang, Ruoruo Wang, Yumei Tan, Yongxiang Liu, Xiyi Ren, Congtao Guo, Rongyu Li, Ming Li","doi":"10.3390/plants14132001","DOIUrl":"10.3390/plants14132001","url":null,"abstract":"<p><p>Citral, an organic compound found in lemongrass (<i>Cymbopogon citratus</i>) oil and <i>Litsea cubeba</i> essential oil, has been reported to exhibit notable antifungal activity against <i>Magnaporthe oryzae</i> (<i>M. oryzae</i>), the pathogen of rice blast, which causes significant economic losses in rice production. However, the role of citral in inducing oxidative stress related to antifungal ability and its underlying regulatory networks in <i>M. oryzae</i> remain unclear. In this study, we investigated the oxidative effects of citral on <i>M. oryzae</i> and conducted transcriptomic and widely targeted metabolomic (WTM) analyses on the mycelia. The results showed that citral induced superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) activities but reduced glutathione S-transferase (GST) activity with 25% maximal effective concentration (EC₂₅) and 75% maximal effective concentration (EC₇₅). Importantly, citral at EC₇₅ reduced the activities of mitochondrial respiratory chain complex I, complex III and ATP content, while increasing the activity of mitochondrial respiratory chain complex II. In addition, citral triggered a burst of reactive oxygen species (ROS) and a loss of mitochondrial membrane potential (MMP) through the observation of fluorescence. Furthermore, RNA-seq analysis and metabolomics analysis identified a total of 466 differentially expression genes (DEGs) and 32 differential metabolites (DAMs) after the mycelia were treated with citral. The following multi-omics analysis revealed that the metabolic pathways centered on AsA, GSH and melatonin were obviously suppressed by citral, indicating a disrupted redox equilibrium in the cell. These findings provide further evidences supporting the antifungal activity of citral and offer new insights into the response of <i>M. oryzae</i> under oxidative stress induced by citral.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12252439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silicon Nanoparticles Alter Soybean Physiology and Improve Nitrogen Fixation Potential Under Atmospheric Carbon Dioxide (CO₂).","authors":"Jingbo Tong","doi":"10.3390/plants14132009","DOIUrl":"10.3390/plants14132009","url":null,"abstract":"<p><p>The interactive effects between nano-silicon dioxide (<i>n</i>-SiO₂) and elevated CO₂ (eCO₂; 645 ppm) on soybean physiology, nitrogen fixation, and nutrient dynamics under climate stress remain underexplored. This study elucidates their combined effects under ambient (aCO₂; 410 ppm) and eCO₂ conditions. eCO₂ + <i>n</i>-SiO₂ synergistically enhanced shoot length (30%), total chlorophyll (112.15%), and photosynthetic rate (103.23%), alongside improved stomatal conductance and intercellular CO₂ (17.19%), optimizing carbon assimilation. Nodulation efficiency increased, with nodule number and biomass rising by 48.3% and 53.6%, respectively, under eCO₂ + <i>n</i>-SiO₂ versus aCO₂. N-assimilation enzymes (nitrate reductase, nitrite reductase, glutamine synthetase, glutamate synthase) surged by 38.5-52.1%, enhancing nitrogen metabolism. Concurrently, phytohormones (16-21%) and antioxidant activities (15-22%) increased, reducing oxidative markers (18-22%), and bolstering stress resilience. Nutrient homeostasis improved, with P, K, Mg, Cu, Fe, Zn, and Mn elevating in roots (13-41%) and shoots (13-17%), except shoot Fe and Zn. These findings demonstrate that <i>n</i>-SiO₂ potentiates eCO₂-driven benefits, amplifying photosynthetic efficiency, nitrogen fixation, and stress adaptation through enhanced biochemical and nutrient regulation. This synergy underscores <i>n</i>-SiO₂ role in optimizing crop performance under future CO₂-rich climates, advocating nano-fertilizers as sustainable tools for climate-resilient agriculture.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12252044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Biocontrol and Growth-Promoting Potential of <i>Penicillium</i> spp. and <i>Trichoderma</i> spp. in Sustainable Agriculture.","authors":"Wenli Sun, Mohamad Hesam Shahrajabian, Lijie Guan","doi":"10.3390/plants14132007","DOIUrl":"10.3390/plants14132007","url":null,"abstract":"<p><p>Plant-growth-promoting fungi (PGPF) play a central role in promoting sustainable agriculture by improving plant growth and resilience. The aim of this literature review is to survey the impacts of <i>Trichoderma</i> spp. and <i>Penicillium</i> spp. on various agricultural and horticultural plants. The information provided in this manuscript was obtained from randomized control experiments, review articles, and analytical studies and observations gathered from numerous literature sources such as Scopus, Google Scholar, PubMed, and Science Direct. The keywords used were the common and Latin names of various agricultural and horticultural species, fungal endophytes, plant-growth-promoting fungi, <i>Trichoderma</i>, <i>Penicillium</i>, microbial biostimulants, and biotic and abiotic stresses. Endophytic fungi refer to fungi that live in plant tissues throughout part of or the entire life cycle by starting a mutually beneficial symbiotic relationship with its host without any negative effects. They are also capable of producing compounds and a variety of bioactive components such as terpenoids, steroids, flavonoids, alkaloids, and phenolic components. <i>Penicillium</i> is extensively known for its production of secondary metabolites, its impact as a bioinoculant to help with crop productivity, and its effectiveness in sustainable crop production. The plant-growth-promotion effects of <i>Trichoderma</i> spp. are related to better absorption of mineral nutrients, enhanced morphological growth, better reproductive potential and yield, and better induction of disease resistance. Both <i>Penicillium</i> spp. and <i>Trichoderma</i> spp. are effective, affordable, safe, and eco-friendly biocontrol agents for various plant species, and they can be considered economically important microorganisms for both agricultural and horticultural sciences. The present review article aims to present the most up-to-date results and findings regarding the practical applications of two important types of PGPF, namely <i>Penicillium</i> spp., and <i>Trichoderma</i> spp., in agricultural and horticultural species, considering the mechanisms of actions of these species of fungi.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12251682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Widely Targeted Metabolomics Reveals Metabolic Divergence in <i>Abutilon theophrasti</i> Populations Under Glufosinate Ammonium Treatment.","authors":"Xiaotong Guo, Yu Wang, Yulian Guo, Chan Luo, Keqiang Cong","doi":"10.3390/plants14131994","DOIUrl":"10.3390/plants14131994","url":null,"abstract":"<p><p><i>Abutilon theophrasti</i> Medikus, a pervasive weed infesting transgenic corn fields, exhibits increasing tolerance to glufosinate ammonium-a widely used herbicide in genetically modified cropping systems. This study employed a widely targeted metabolomics approach to investigate differential metabolic responses to glufosinate ammonium across two <i>Abutilon theophrasti</i> populations under identical treatments. A total of 2546 metabolites were detected, predominantly classified into alkaloids, amino acids and derivatives, and flavonoids, among other categories. Three pivotal metabolic pathways (Arginine and proline metabolism, Biosynthesis of amino acids, D-amino acid metabolism) were identified as critical regulators of herbicide response. These findings advance our understanding of weed metabolic adaptation to glufosinate ammonium and lay a foundation for elucidating potential herbicide resistance mechanisms in weeds.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12251701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological Responses of Asparagus Plants to Soil Disinfection Strategies Targeting Asparagus Decline Syndrome.","authors":"Francisco Javier López-Moreno, Eloy Navarro-León, Miguel de Cara, Teresa Soriano, Juan Manuel Ruiz","doi":"10.3390/plants14131992","DOIUrl":"10.3390/plants14131992","url":null,"abstract":"<p><p>Asparagus decline syndrome (ADS) poses a significant threat to asparagus cultivation worldwide. To address this challenge, a two-year investigation was carried out in Spain to assess the impacts of three soil disinfection strategies on asparagus crops. These included biofumigation with <i>Brassica carinata</i> seed pellets, biofumigation using poultry manure pellets, and chemical disinfection with dazomet. In addition to evaluating the potential of these treatments to alleviate ADS, the research also focused on identifying the physiological changes linked to the syndrome by examining indicators of oxidative metabolism, hormonal equilibrium, and phenolic compound profiles. Among the treatments evaluated, biofumigation with <i>B. carinata</i> pellets enhanced vegetative growth, photosynthetic pigment accumulation, antioxidant capacity, and hormonal homeostasis, with these improvements becoming more pronounced in the second year. This approach appeared to promote a healthier physiological status in asparagus plants, likely through improved soil health and reduced biotic and abiotic stress perception. In contrast, chemical disinfection with dazomet, despite initially stimulating some physiological responses, was associated with elevated oxidative stress. Overall, the findings suggest that organic-based soil treatments, particularly <i>B. carinata</i> biofumigation, represent a promising strategy to strengthen asparagus vigor and resilience against ADS. Further studies are needed to assess their long-term effects in perennial cultivation systems.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12252399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild Species from the Asteraceae Family, Traditionally Consumed in Some Mediterranean Countries.","authors":"Ekaterina Kozuharova, Giuseppe Antonio Malfa, Rosaria Acquaviva, Benito Valdés, Daniela Batovska, Christina Stoycheva, Moh Rejdali, Pasquale Marino, Vivienne Spadaro","doi":"10.3390/plants14132006","DOIUrl":"10.3390/plants14132006","url":null,"abstract":"<p><p>Mediterranean countries represent a dynamic hub of cultural exchange, where wild plants play a significant role in culinary traditions. A substantial number of these plants belong to the Asteraceae family. The climate similarities across the region contribute to the common distribution ranges of the plants. While many species are widely distributed, others are confined to specific subregions, such as the western Mediterranean, eastern Mediterranean, or North Africa. Only six taxa of the traditionally consumed wild Asteraceae plants are endemic to just one country. This review focuses on wild plants from the Asteraceae family traditionally used as food across 13 study sites, comprising 11 countries in the Mediterranean and adjacent territories, including both mainland areas and three islands. The objective is to identify and analyze patterns of native distribution in relation to actual consumption. As a result, 167 edible wild plants from the Asteraceae family were identified. Their patterns of distribution and consumption are described and analyzed. The highest number of these edible wild plants from the Asteraceae family is consumed in Spain (<i>n</i> = 65), followed by southern Italy (<i>n</i> = 44) and Morocco (<i>n</i> = 32). A similar pattern of consumption is seen in Turkey (<i>n</i> = 24), Sicily (<i>n</i> = 23), Jordan and Palestine (<i>n</i> = 21), and Bulgaria (<i>n</i> = 21). It is notable that 106 plants are used as food in one particular country only, although most of them are distributed in several other countries. Many of the species consumed in certain countries are not used by neighboring populations, highlighting a limited cross-border transmission of ethnobotanical knowledge. The findings from a Jaccard index statistical analysis are discussed.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12251735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Identification and Salt Stress-Responsive Expression Analysis of the <i>GmPLATZ</i> Gene Family in Soybean (<i>Glycine max</i> L.).","authors":"Mingyu Wang, Zheyun Guan, Songquan Wu, Jingyong Zhang, Chunjing Lin, Yanyan Sun, Mingzhe Shen, Chunbao Zhang","doi":"10.3390/plants14132004","DOIUrl":"10.3390/plants14132004","url":null,"abstract":"<p><p>The plant-specific <i>PLATZ</i> transcription factors play crucial roles in plant growth, development, and responses to abiotic stresses. However, despite their functional significance, <i>PLATZ</i> genes remain poorly characterized in soybeans. In this study, we conducted a genome-wide analysis of the <i>GmPLATZ</i> gene family and investigated their expression profiles under salt stress. We identified a total of 29 <i>GmPLATZ</i> genes in the soybean genome and systematically analyzed their physicochemical properties, conserved domains, evolutionary relationships, cis-acting elements, and expression regulation patterns. Subcellular localization predictions indicated nuclear localization for most <i>GmPLATZs</i>, except for <i>GmPLATZ5</i> and <i>GmPLATZ14</i>, which showed dual chloroplast-nuclear localization. A gene family expansion analysis indicated that 21 segmental duplication events were the primary driver of <i>GmPLATZ</i> diversification. A phylogenetic analysis classified the <i>GmPLATZ</i> genes into four subgroups, while gene structure and motif analyses revealed conserved zinc-binding domains and identified multiple cis-acting elements associated with light responsiveness, hormone signaling, and stress responses. Expression profiling showed tissue-specific expression patterns, with 13 <i>GmPLATZ</i> genes differentially expressed under salt stress, including root-preferential members (e.g., <i>GmPLATZ1</i>, <i>GmPLATZ10</i>) and leaf-preferential members (e.g., <i>GmPLATZ8</i>, <i>GmPLATZ9</i>). This study provides a theoretical basis for further investigation of <i>GmPLATZ</i> gene functions in soybean development and stress tolerance.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12251706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Pancreatic Lipase Inhibitory Activities of <i>Panax japonicus</i> (T. Nees) C.A. Meyer.","authors":"Jinfeng Yang, Wenxuan Jiang, Ju Hee Park, Eun Soo Seong, Yong Soo Kwon, Myong Jo Kim","doi":"10.3390/plants14132003","DOIUrl":"10.3390/plants14132003","url":null,"abstract":"<p><p>Obesity and its associated complications, including oxidative stress, pose significant global health challenges. Natural products offer a promising avenue for developing novel therapeutic strategies. In this study, we investigated the potential of <i>Panax japonicus</i> (T. Nees) C.A. Meyer, a traditional medicinal plant known for its antioxidant and anti-obesity properties. A methanol extract of <i>Panax japonicus</i> and its fractions were evaluated for their in vitro antioxidant activities (tested using DPPH and reducing power assays), pancreatic lipase (PL) inhibitory capacities, and underlying mechanisms of action. The results indicated that the ethyl acetate fraction of <i>P. japonicus</i> (PJEA) exhibited the greatest potency, demonstrating strong antioxidant activity and significantly inhibiting digestive enzyme activity (pancreatic lipase). Mechanistic studies revealed that the PL inhibition was of a mixed type, combining both competitive and non-competitive mechanisms. Furthermore, PJEA demonstrated the ability to inhibit the differentiation of preadipocytes, primarily exerting its anti-adipogenic effects by downregulating the mRNA expression of PPARγ and the gene expression of C/EBPα. In addition, the extract suppressed the gene expression of FAS and ACC in adipose tissue. Isolation of the bioactive compounds from PJEA identified kaempferol 3-O-α-L-rhamnoside and catechin, which potentially contribute to the observed anti-obesity effects. Overall, this study highlights <i>P. japonicus</i> as a promising natural ingredient for scavenging free radicals and managing obesity, suggesting its potential for development into functional foods or therapeutic agents.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 13","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12252066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}