Plants-Basel最新文献

{"title":"In Vitro and In Vivo Antitumor Activity of a Chloroform Partition from <i>Ibervillea sonorae</i> (S. Watson) GreeneEndophytic <i>Bacillus subtilis</i> Extracts.","authors":"Ricardo Romero-Arguelles, César Iván Romo-Sáenz, Patricia Tamez-Guerra, Diego Fonseca-Rivera, Joel H Elizondo-Luevano, Nancy Edith Rodriguez-Garza, Orquidea Perez-Gutierrez, Celia María Quiñones-Flores, Carlos Arzate-Quintana, Lydia Paulina Loya Hernandez, Ricardo Gomez-Flores","doi":"10.3390/plants14101474","DOIUrl":"https://doi.org/10.3390/plants14101474","url":null,"abstract":"<p><p>Cancer is a complex disease due to its high variability and resistance to conventional treatments. The search for new therapies has prompted the study of less invasive natural sources, such as endophytic bacteria from medicinal plants. <i>Bacillus subtilis</i> is known to produce bioactive metabolites with promising pharmacological properties. This study evaluated the antitumor activity of the endophyte <i>B. subtilis</i> from <i>Ibervillea sonorae</i> against murine L5178Y-R lymphoma cells within in vitro and in vivo models. <i>B. subtilis</i> methanol extract was fractionated in hexane, chloroform, and methanol, with the chloroform partition showing the highest tumor cell growth inhibition (IC₅₀ = 34.62 ± 0.180 µg/mL) and the highest selectivity index (SI = 15.53) when compared with the hexane and methanol partitions. The in vivo study showed that mice treated with 10 mg/kg of the chloroform partition significantly (<i>p</i> < 0.01) reduced the tumor volume and weight without affecting tumor-free body weight. The maximum tolerated dose test indicated that 10 mg/kg was safe and well tolerated. These results indicate that <i>B. subtilis</i> may be a promising source of selective antitumor compounds.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of Photosynthesis and Chlorophyll Fluorescence to Nitrogen Changes in Rice with Different Nitrogen Use Efficiencies.","authors":"Zexin Qi, Chen Xu, Rui Tang, Qiang Zhang, Wenzheng Sun, Chenglong Guan, Ye Wang, Mengru Zhang, Jiale Ding, Yuankai Zhang, Hong Yang, Ying Yang, Xiaolong Liu, Zhian Zhang, Fenglou Ling","doi":"10.3390/plants14101465","DOIUrl":"https://doi.org/10.3390/plants14101465","url":null,"abstract":"<p><p>Nitrogen is a key element in promoting crop growth and development and improving photosynthesis. This study aimed to study the response of two rice genotypes to the restoration of N supply after varying periods of N deficiency. We used the low-nitrogen-tolerant rice Jijing 88 (JJ 88) and the nitrogen-sensitive rice variety Xinong 999 (XN 999) as test materials. The results of this study indicated that, compared to XN 999, JJ 88 has a higher content of the photosynthetic pigments. Photosynthesis in JJ 88 has strong adaptability under low-nitrogen conditions. Upon an increase in the nitrogen supply level, the maximum regeneration rate of ribulose biphosphate (RuBP, <i>J_max</i>) and the maximum carboxylation rate of RuBP (<i>V_cmax</i>) in JJ 88 showed a relatively large increase. The chlorophyll fluorescence parameters, including the effective quantum yield of photosystem II (Φ_PSII), the efficiency of excitation capture by open PSII centers (Fv'/Fm'), photochemical fluorescence quenching (qP), and the electron transfer rate (ETR) decreased slightly, while the non-photochemical fluorescence quenching (NPQ) increased slightly. Under low-nitrogen conditions, low-nitrogen-tolerant rice varieties maintain reasonable growth during the seedling stage. With an increase in the nitrogen supply level, the dry matter accumulation, photosynthetic pigment content, photosynthesis, and electron transfer ability of plants improve, but not to normal nitrogen supply levels. However, compared with XN 999, JJ 88 has a more proactive recovery ability. The research results provide valuable guidance for the breeding of nitrogen-efficient rice varieties and nitrogen fertilizer management.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity and Conservation of <i>Bomarea ovallei</i> (Phil.) Ravenna: Microsatellite Markers Reveal Population Vulnerability in the Atacama Desert.","authors":"Valeska Rozas-Lazcano, Mariel Mamani-Gómez, Irina Rojas-Jopia, Mariana Arias-Aburto, Roberto Contreras-Díaz","doi":"10.3390/plants14101468","DOIUrl":"https://doi.org/10.3390/plants14101468","url":null,"abstract":"<p><p>The Atacama Desert, the driest and oldest desert on Earth, hosts a unique floral phenomenon known as the Desierto Florido (Flowering Desert), which occurs sporadically in response to rare rainfall events. <i>Bomarea ovallei</i> (Phil.) Ravenna is an endemic and endangered species of the Atacama Desert. However, its populations are geographically restricted and potentially vulnerable to genetic erosion due to isolation and extreme environmental conditions. This study aims to assess the genetic diversity of <i>B. ovallei</i> populations and develop microsatellite markers using next-generation sequencing (NGS) technology. A total of 268 microsatellite loci were identified, and 34 co-dominant markers were successfully developed for the first time in <i>B. ovallei</i>. Genetic diversity analysis using eight fluorescently labeled SSR markers revealed low genetic diversity across four populations, with the highest diversity observed in the QCA population, located within Llanos de Challe National Park, and the lowest in the TOTO population, which is highly exposed to anthropogenic activities. UPGMA and STRUCTURE analyses revealed three genetic clusters and high admixture among populations, suggesting historical or ongoing gene flow despite geographical separation. The presence of non-polymorphic loci and low PIC values in some markers further supports limited genetic variation. The newly developed microsatellite markers offer a valuable tool for future genetic studies, enabling the monitoring of genetic diversity and informing strategies for the preservation of this rare and ecologically significant species.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity of Essential Oils of <i>Origanum vulgare</i>, <i>Salvia rosmarinus</i>, and <i>Salvia officinalis</i> Against <i>Aculops lycopersici</i>.","authors":"Thomas Giordano, Giuliano Cerasa, Ilaria Marotta, Mauro Conte, Santo Orlando, Adele Salamone, Michele Massimo Mammano, Carlo Greco, Haralabos Tsolakis","doi":"10.3390/plants14101462","DOIUrl":"https://doi.org/10.3390/plants14101462","url":null,"abstract":"<p><p>The tomato russet mite (TRM), <i>Aculops lycopersici</i>, is a destructive pest of tomato crops worldwide. It poses a significant challenge to growers in both greenhouse and open-field conditions. Traditional chemical control methods are often ineffective, promote resistance, and have negative environmental impacts. This has prompted the search for alternative strategies, such as biological control and eco-friendly botanical pesticides. In this study, we evaluated the acaricidal effects of essential oils (EOs) extracted from three officinal plants, <i>Origanum vulgare</i> L., <i>Salvia rosmarinus</i> Spenn., and <i>Salvia officinalis</i> L., cultivated using precision aromatic crop (PAC) techniques. Their efficacy was evaluated against <i>A. lycopersici</i> under laboratory conditions. The chemical composition of the EOs was determined by solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The dominant component of <i>O. vulgare</i> EO was carvacrol (83.42%), followed by ρ-cymene (3.06%), and γ-terpinene (2.93%). In <i>S. rosmarinus</i>, α-pinene (28.0%), 1,8-cineole (11.00%), and borneol (7.72%) were the major components. <i>S. officinalis</i> EO was characterized by high levels of 1,8-cineole (27.67%), camphor (21.91%), and crisantenone (12.87%). We tested multiple concentrations (320-5000 μL L-¹) and exposure times (1-4 days) to assess mite mortality. The results revealed both dose- and time-dependent toxic activity, with significant differences among EOs. <i>O. vulgare</i> EO was the most toxic, causing 90% mortality at 0.5% (<i>w</i>/<i>v</i>) concentration after 4 days. <i>S. rosmatinus</i> and <i>S. officinalis</i> EOs had more limited effects, with 46% and 42% mortality, respectively. Lethal concentration (LC₅₀) values were 2.23 mL L-¹ (95% CI: 1.74-3.05) for <i>O. vulgare</i>, 5.84 mL L-¹ (95% CI: 3.28-22.29) for <i>S. rosmarinus</i>, and 6.01 mL L-¹ (95% CI: 2.63-261.60) for <i>S. officinalis</i>. These results indicate that <i>O. vulgare</i> EO shows efficacy comparable to commercially available botanical pesticides. Our findings support the potential of <i>O. vulgare</i> EO as a viable alternative for the control of <i>A. lycopersici</i>, contributing to integrated pest management (IPM) strategies.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into the Influence of Ecological Factors on Shaping Distribution Patterns of <i>Camptotheca acuminata</i> for Conservation and Management.","authors":"Taojing Wang, Yuchen Li, Teja Manda, Jie Lin, Tianyu Huang, Yao Zhang, Liming Yang","doi":"10.3390/plants14101466","DOIUrl":"https://doi.org/10.3390/plants14101466","url":null,"abstract":"<p><p><i>Camptotheca acuminata</i> Decne. is an endemic and valuable tree species in China that is renowned for its medicinal and economic value due to secondary metabolites like camptothecin, a potent anti-cancer compound. With wild resources dwindling, it is a key protected species. Predicting and analyzing its suitable habitats under different future environmental scenarios is essential for conservation, introduction, development, and planting strategies. This study used 1008 distribution points and 32 environmental factors, applying the MaxEnt v3.4.4 model and ArcGIS v10.7 software to predict <i>C. acuminata</i>'s potential distribution under four greenhouse gas emission scenarios (RCP2.6, RCP4.5, RCP6.0, and RCP8.5) for the present, 2050, and 2070. This study identifies the key environmental factors influencing its distribution and analyzes habitat trends under various ecological scenarios. The dominant environmental factors are Bio6 (contribution 23%; importance 59.8%), human activity factor (contribution 18.6%; importance 15.7%), Slope2 (contribution 1%; importance 7%), Slope3 (contribution 5.1%; importance 3.4%), elevation (contribution 0.9%; importance 1.7%), and Bio14 (contribution 41.2%; importance 1%). The total potential suitable habitat area for <i>C. acuminata</i> is 1.5796 × 10⁴ km². Except under RCP8.5, where the habitat area continuously increases, the habitat area shows a trend of first increasing and then decreasing. When human activity is considered, the total potential suitable habitat area is 1.8495 × 10⁴ km², with a consistent decrease under all scenarios except RCP8.5. Centroid migration analysis shows that, driven by global warming, the suitable habitats for <i>C. acuminata</i> are shifting toward higher latitudes. This study provides theoretical support for the conservation, resource management, and germplasm protection of <i>C. acuminata</i> under future ecological and environmental changes.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, Expression Profile Analysis, and Functional Prediction of <i>UGP</i> Gene Family in <i>Dendrocalamus brandisii</i>.","authors":"He Li, Chongyang Wu, Xiangyi Li, Junlei Xu, Zhanchao Cheng, Jian Gao","doi":"10.3390/plants14101458","DOIUrl":"https://doi.org/10.3390/plants14101458","url":null,"abstract":"<p><p>UDP-glucose pyrophosphorylase (UGPase) is essential for carbohydrate metabolism, catalyzing UDP-glucose synthesis, a precursor for sucrose and cellulose biosynthesis. While <i>UGP</i> genes have been widely studied in plants, their functions in <i>Dendrocalamus brandisii</i> remain unclear. This study identified and characterized the <i>DbUGP</i> gene family using the whole genome and transcriptome data of <i>D. brandisii</i>, in conjunction with whole genome data from 10 additional species through sequence alignment, phylogenetic analysis, gene structure and motif exploration, protein structure prediction, and expression profiling. Phylogenetic analysis showed eight identified <i>DbUGPs</i> clustered with two <i>OsUGPs</i> in two clades. Gene structure, motif, and collinearity analyses indicate conservation with other bamboo <i>UGPs</i>. The gene family exhibited segmental duplications. Expression profiling revealed <i>DbUGP1</i>/<i>5</i> were highly expressed in flowers, while others were enriched in shoots, buds, and culms. <i>DbUGP1</i>/<i>4</i>/<i>8</i> were significantly downregulated during culm maturation. Protein structure prediction indicated two conformations with catalytic sites in disordered coil regions. WGCNA identified co-expression modules and protein interaction networks centered on <i>DbUGP1</i>/<i>4</i>, while KEGG enrichment indicated their functions in metabolism, signal transduction, and stress adaptation. Promoter analysis identified cis-regulatory elements responsive to light, MeJA, and ABA. This study suggests that the evolutionarily conserved <i>DbUGPs</i> exhibit mutual coordination and dynamic expression during <i>D. brandisii</i> growth, providing fresh insights into their functional roles.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Functional Analysis of Endophytic Bacteria <i>Bacillus cereus</i> in <i>Sphagnum palustre</i>.","authors":"Hongying Wang, Jiankang Xin, Xiaona Zhang, Shan Jiang","doi":"10.3390/plants14101476","DOIUrl":"https://doi.org/10.3390/plants14101476","url":null,"abstract":"<p><p>Endophytic bacteria in <i>Sphagnum palustre</i> have a growth-promoting effect on plants. In this study, the endophytic bacterium strain J11 in <i>S. palustre</i> was isolated and identified as <i>Bacillus cereus</i>, and its growth cycle, functional characteristics, and effects on maize growth were analyzed. The results indicate that as <i>B. cereus,</i> the growth cycle of J11 consists of four phases, and the logarithmic phase lasts 2~24 h, with the abilities of phosphorus solubilization, protease, IAA, siderophore, and NH₃ production. The phosphorus solubilization ability of J11 ranges from 1.66 ± 0.07 to 1.98 ± 0.07 mg/L, and the IAA production varies from 1.51 ± 0.07 to 8.67 ± 0.16 mg/L. It has a growth-promoting effect on maize by increasing the seed germination rate by 29.27%, plant height by 4.21%, leaf length by 17.12%, leaf width by 29.51%, above-ground fresh weight by 50.79%, below-ground fresh weight by 46.30%, and chlorophyll content by 56.81%. This study represents the first report on the isolation and identification of <i>B. cereus</i> from <i>S. palustre</i>. Furthermore, this study systematically investigated its multiple plant growth-promoting traits and functional characteristics. These findings provide valuable resources and a theoretical foundation for the development and functional exploration of microbial resources in agricultural applications.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous GA₃ Promotes Germination by Reducing Endogenous Inhibitors in Sainfoin (<i>Onobrychis viciifolia</i>) Seeds.","authors":"Yanyan Luo, Kun Wang, Yuheng Yao, Lili Nan","doi":"10.3390/plants14101464","DOIUrl":"https://doi.org/10.3390/plants14101464","url":null,"abstract":"<p><p>Endogenous inhibitors can inhibit seed germination, and GA₃ can promote seed germination. Whether GA₃ can affect the changes in endogenous inhibitors has not been clarified. In order to study the effect of GA₃ on the endogenous inhibitors in sainfoin (<i>Onobrychis viciifolia</i>) seeds, the systematic separation method and gas chromatography-mass pectrometry (GC-MS) method were used to determine the endogenous inhibitors using cabbage (<i>Brassica rapa</i> var. glabra Regel) as a bioassay model to validate the inhibitory activity in sainfoin seeds, and then the optimal concentration of GA₃ was determined to promote seed germination. The results showed that endogenous inhibitors existed in the pod coat, seed coat, and seed embryo of sainfoin seeds, with the methanol and ethyl acetate phases showing the highest degree of inhibition. The organic compounds were mainly organic acids, phenols, lipids, and alkanes. The levels of changes in germination indicators, storage substances, and antioxidant enzymes determined that 600 mg/L GA₃ was the optimum concentration to promote germination of sainfoin seed. It was also found that 600 mg/L GA₃ reduced the relative content of endogenous inhibitors and changed the content of endogenous hormones. In summary, the presence of endogenous inhibitors may be one of the reasons for the low germination rate of sainfoin seeds, with 3-methoxycatechol and 4-nitrosodiphenylamine playing a major role. GA₃ can reduce the relative content and types of endogenous inhibitors to promote the germination of sainfoin seeds. Our experimental results provide the basis for subsequent exploration of the mechanism of specific endogenous inhibitors and the identification of deeper molecular mechanisms.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>CsCBDAS2</i>-Driven Enhancement of Cannabinoid Biosynthetic Genes Using a High-Efficiency Transient Transformation System in <i>Cannabis sativa</i> 'Cheungsam'.","authors":"Sang-Cheol Baek, Sang-Yoon Jeon, Bo-Hyun Byun, Da-Hoon Kim, Ga-Ram Yu, Hyuck Kim, Dong-Woo Lim","doi":"10.3390/plants14101460","DOIUrl":"https://doi.org/10.3390/plants14101460","url":null,"abstract":"<p><p><i>Cannabis sativa</i> produces pharmacologically valuable cannabinoids. In this study, we developed and optimized a transient transformation system using <i>Cannabis sativa</i> 'Cheungsam' to facilitate gene functional analysis. Various experimental conditions, including plant developmental stages, light conditions, <i>Agrobacterium</i> strains, tissue types, and physical treatments such as sonication and vacuum infiltration, were systematically evaluated using GUS histochemical staining and qPCR analysis. Among these, 7-day-old seedlings cultured under dark conditions and transformed with the GV3101 strain exhibited high transformation efficiency. Leaf tissue showed a higher GUS staining proportion and GUS staining area compared to hypocotyl and cotyledon tissues. The application of a combination of sonication and vacuum infiltration techniques resulted in the most intense GUS expression. Using the optimized protocol, we introduced a recombinant vector carrying <i>CsCBDAS2</i>, a key gene in cannabidiol (CBD) biosynthesis. qPCR analysis revealed that <i>CsCBDAS2</i> overexpression led to significant upregulation of multiple upstream CBD biosynthetic genes (<i>CsOAC</i>, <i>CsGOT</i>, <i>CsPT1</i>, <i>CsPT4</i>, <i>CsCBDAS1</i>, and <i>CsCBDAS2</i>) and the transcription factor (TF) <i>CsWRKY20</i>, suggesting coordinated co-expression and potential involvement of a transcriptional feedback loop. These results demonstrate the effectiveness of our transient transformation system and provide insights into the regulatory mechanisms of cannabinoid biosynthesis in <i>cannabis</i>.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pantoea stewartii</i> subsp. <i>stewartii</i> an Inter-Laboratory Comparative Study of Molecular Tests and Comparative Genome Analysis of Italian Strains.","authors":"Valeria Scala, Nicoletta Pucci, Riccardo Fiorani, Alessia L'Aurora, Alessandro Polito, Marco Di Marsico, Riccardo Aiese Cigliano, Eleonora Barra, Serena Ciarroni, Francesca De Amicis, Salvatore Fascella, Francesca Gaffuri, Andreas Gallmetzer, Francesca Giacobbi, Pasquale Domenico Grieco, Valeria Gualandri, Giovanna Mason, Daniela Pasqua di Bisceglie, Domenico Rizzo, Maria Rosaria Silletti, Simona Talevi, Marco Testa, Cosimo Tocci, Stefania Loreti","doi":"10.3390/plants14101470","DOIUrl":"https://doi.org/10.3390/plants14101470","url":null,"abstract":"<p><p><i>Pantoea stewartii</i> subsp. <i>stewartii</i> (Pss) is a Gram-negative bacterium causing Stewart wilt, a severe disease in maize. Native to North America, it has spread globally through the maize seed trade. Resistant maize varieties and insecticides are crucial to mitigate the disease's economic impact. Pss is a quarantine pest, requiring phytosanitary certification for the seed trade in European countries. Accurate diagnostic tests, including real-time PCR, are fundamental to detect Pss and distinguish it from other bacteria, like <i>Pantoea stewartii</i> subsp. <i>indologenes</i> (Psi), a non-quarantine bacteria associated with maize seeds. Population genetics is a valuable tool for studying adaptation, speciation, population structure, diversity, and evolution in plant bacterial pathogens. In this study, the key activities of interlaboratory comparisons are reported to assess diagnostic sensitivity (DSE), diagnostic specificity (DSP) and accuracy (ACC) for different real-time PCR able to detect Pss in seeds. The results of complete sequencing of Italian bacterial isolates are presented. This study enhances our understanding of molecular methods for diagnosing and identifying pathogens in maize seeds, improving knowledge of Pss genomes to prevent their spread and trace possible entry routes from endemic to non-endemic areas.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}