Food Analytical Methods最新文献_第10页

Retraction Note: Development of an Upconversion Luminescence Nanoparticles–Based Immunochromatographic Assay for the Rapid Detection of Dexamethasone in Animal Tissue 撤稿说明：基于上转换发光纳米粒子的免疫层析测定的开发，用于快速检测动物组织中的地塞米松

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-08-02 DOI: 10.1007/s12161-024-02655-0

Shiwei Zhang, Tianqi Yao, Shifeng Wang, Ronghu Feng, Liqiong Chen, Vivian Zhu, Guiping Hu, Heng Zhang, Guowu Yang

引用次数: 0

Electrochemical Characterization and Detection of Ascorbic Acid in Garlic Using Activated Glassy Carbon Electrode: a Comprehensive Study 利用活性玻璃碳电极进行电化学表征和检测大蒜中的抗坏血酸：一项综合研究

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-08-02 DOI: 10.1007/s12161-024-02660-3

Dereje Yenealem, Dessie Eyayu, Dessie Tibebe, Marye Mulugeta, Yezbie Kassa, Zerubabel Moges, Feleke Kerebih, Tarekegn Fentie, Agmas Amare, Menilek Ayalew

{"title":"Electrochemical Characterization and Detection of Ascorbic Acid in Garlic Using Activated Glassy Carbon Electrode: a Comprehensive Study","authors":"Dereje Yenealem, Dessie Eyayu, Dessie Tibebe, Marye Mulugeta, Yezbie Kassa, Zerubabel Moges, Feleke Kerebih, Tarekegn Fentie, Agmas Amare, Menilek Ayalew","doi":"10.1007/s12161-024-02660-3","DOIUrl":"10.1007/s12161-024-02660-3","url":null,"abstract":"<div><p>This study delves into the electrochemical properties of ascorbic acid (AA) in garlic bulbs, employing both a glassy carbon electrode (GCE) and an activated glassy carbon electrode (AGCE). Cyclic voltammetry (CV) and square wave voltammetry (SWV) were utilized for the thorough characterization and detection of AA in freshly harvested garlic bulbs sourced from Debark, Ethiopia. The AGCE was meticulously prepared through a 200-s activation process at a potential of 1750 mV. Demonstrating remarkable electrocatalytic behavior towards AA, the AGCE exhibited enhanced peak current and a less positive shift in peak potential compared to the GCE. It demonstrated an increase with pH up to 6.5, followed by a decrease beyond pH 6.5, leading to the selection of pH 6.5 as the optimal value. The variation in scan rate indicated an adsorption-controlled process. The established calibration curve equation was <i>I</i>p (μA) = − 9.94 – 0.15CAA, presenting a high <i>R</i><sup>2</sup> value of 0.999 within the linear range of 0.01 – 0.2 mM. The method demonstrated a low limit of detection (0.004 mM) and quantification (0.012 mM). A robust degree of recovery (102.2%) validated the method’s accuracy. The concentration of AA in fresh garlic bulbs was determined to be 192.8 mg/kg, affirming the method’s suitability for the analysis of real environmental samples.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 10","pages":"1473 - 1483"},"PeriodicalIF":2.6,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141886459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UPLC-PDA Phenolic Compounds Profile of Mango Peel Extracts Obtained using Different Solvents and Ultrasound-Assisted Extraction 使用不同溶剂和超声辅助萃取法提取的芒果皮提取物的 UPLC-PDA 酚类化合物谱图

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-07-29 DOI: 10.1007/s12161-024-02651-4

Verónica Marcillo-Parra, Diego Santiago Tupuna-Yerovi, Maritza Molina, Karolay Abigail Hernández Balladares, Jenny Ruales

{"title":"UPLC-PDA Phenolic Compounds Profile of Mango Peel Extracts Obtained using Different Solvents and Ultrasound-Assisted Extraction","authors":"Verónica Marcillo-Parra, Diego Santiago Tupuna-Yerovi, Maritza Molina, Karolay Abigail Hernández Balladares, Jenny Ruales","doi":"10.1007/s12161-024-02651-4","DOIUrl":"10.1007/s12161-024-02651-4","url":null,"abstract":"<div><p>This study evaluated the performance of two techniques – conventional solvent extraction (CSE) and ultrasound-assisted extraction (UAE) – using different solvents (50% methanol, 50% ethanol, 70% acetone) for the extraction of phenolics from mango peel. For each mango peel extract (MPE), a further purification step was performed on an OASIS HLB 6 cc VAC-packed column. Subsequently, the phenolic compound profile was quantified using Ultra Performance Liquid Chromatography accoupled with a Photodiode Array (UPLC-PDA), and the antioxidant activity was also assessed. The results indicated that using 50% methanol as an extraction solvent yielded more significant phenolics, such as mangiferin, gallic acid, quercetin, and rutin. Additionally, the extracts obtained by UAE with different solvents showed a higher phenolic compound content, with mangiferin being the most predominant, mainly when methanol was used (222.34 mg/100 g DW) and higher antioxidant activity than extracts obtained by CSE, especially when acetone was used (364.00 ± 13.00 µmol Trolox/g DW). Overall, the results indicate the potential use of these mango by-products as a source of natural ingredients for the development of functional food.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 10","pages":"1466 - 1472"},"PeriodicalIF":2.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141869628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Effect of Temperature and Pooling in Detection and Isolation of the Major Non-O157 Shiga Toxin–Producing Escherichia coli Serogroups from Meat Samples with the Use of Alternative and Standard Methods 评估使用替代方法和标准方法检测和分离肉类样本中主要非 O157 产志贺毒素大肠埃希氏菌血清群时温度和汇集的影响

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-07-24 DOI: 10.1007/s12161-024-02653-2

Marion Bouvier, Camille Thollet, Thomas Douellou, Delphine Thevenot Sergentet

{"title":"Evaluating the Effect of Temperature and Pooling in Detection and Isolation of the Major Non-O157 Shiga Toxin–Producing Escherichia coli Serogroups from Meat Samples with the Use of Alternative and Standard Methods","authors":"Marion Bouvier, Camille Thollet, Thomas Douellou, Delphine Thevenot Sergentet","doi":"10.1007/s12161-024-02653-2","DOIUrl":"10.1007/s12161-024-02653-2","url":null,"abstract":"<div><p>Pathogenic Shiga toxin–producing <i>Escherichia coli</i> (STEC) are an important cause of foodborne illness. The detection of STEC in finished products and during the manufacturing process has an important role as part of verification plans, to confirm that practices and procedures described in the food safety program are successfully applied to control STEC. The aim of this study is to evaluate the effect of temperature and pooling in detection and isolation of the major non-O157 STEC serogroups from meat samples with the use of alternative and standard methods. Bovine meat was experimentally inoculated with one of the “Top 6” non-O157 STEC strains (O26, O103, O111, O145, O45, and O121). Both ISO TS 13136:2012 and a novel alternative method were implemented to evaluate the impact of temperature and pooling. An increase of the enrichment temperature to 41.5 °C allowed the detection of the spiked strain in 10% more samples compared to enrichment at 37 °C. The use of 25- and 375-g sample tests demonstrated no statistically differences between both methods. And finally, this alternative method appears easy to use and time-saving for routine laboratory use.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 10","pages":"1455 - 1465"},"PeriodicalIF":2.6,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12161-024-02653-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141776842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Free Fatty Acids in Foodstuff by Solid Phase Extraction and Gas Chromatography-Flame Ionization Detection Using H2SO4-MeOH Derivatization 利用 H2SO4-MeOH 衍生技术，采用固相萃取和气相色谱-火焰离子化检测法测定食品中的游离脂肪酸

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-07-19 DOI: 10.1007/s12161-024-02652-3

Cao Wenming, Zhang Yuyang, Wei Tingting, Li Tong, Yang Guolong

{"title":"Determination of Free Fatty Acids in Foodstuff by Solid Phase Extraction and Gas Chromatography-Flame Ionization Detection Using H2SO4-MeOH Derivatization","authors":"Cao Wenming, Zhang Yuyang, Wei Tingting, Li Tong, Yang Guolong","doi":"10.1007/s12161-024-02652-3","DOIUrl":"10.1007/s12161-024-02652-3","url":null,"abstract":"<div><p>Free fatty acids (FFAs) serve as crucial analytical markers in oils, fats, and food, reflecting food safety through their content variations. However, the analysis of FFAs in food samples is constrained by potential interferences from other acidic components in the sample matrix during sample processing, resulting in distorted analytical outcomes and introducing positive bias. The established method was also validated in terms of linearity, limits of detection (LOD) and quantification (LOQ), and matrix effect. It was proved to be linear (R<sup>2</sup> > 0.99), with lower LODs of 0.108–0.564 μg/mL, and lower LOQs of 0.258–1.541 μg/mL for all FFAs. Overall, the simple, rapid, and cost-effective method developed in this study provides a potential application for the extraction and enrichment of FFAs in complex sample matrices, including edible oils, fatty foods, and high-content biological samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 10","pages":"1446 - 1454"},"PeriodicalIF":2.6,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141740683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green Extraction of Anthocyanins from Prunus domestica Using Natural Deep Eutectic Solvents: Anti-sickling Properties and Phenolic Impact Assessment 使用天然深共晶溶剂绿色提取梅花中的花青素：抗酸洗特性和酚类影响评估

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-07-12 DOI: 10.1007/s12161-024-02650-5

Jagdish S. Patil, Pravin O. Patil, Sopan N. Nangare, Rakesh E. Mutha, Sanjaykumar B. Bari, Zamir G. Khan

{"title":"Green Extraction of Anthocyanins from Prunus domestica Using Natural Deep Eutectic Solvents: Anti-sickling Properties and Phenolic Impact Assessment","authors":"Jagdish S. Patil, Pravin O. Patil, Sopan N. Nangare, Rakesh E. Mutha, Sanjaykumar B. Bari, Zamir G. Khan","doi":"10.1007/s12161-024-02650-5","DOIUrl":"10.1007/s12161-024-02650-5","url":null,"abstract":"<div><p>Natural Deep Eutectic Solvents (NADESs) are a form of green solvents used to extract phenolic compounds and are considered a more environmentally friendly alternative to traditional solvents. The study aimed to explore a more eco-friendly and effective approach to extracting anthocyanins from <i>Prunus domestica</i> by utilizing NADESs. Four NADESs were created by making combinations of choline chloride with citric acid, lactic acid, glycerol, and ethylene glycol. The study investigated the anthocyanins profile, antioxidant, and anti-sickling properties of fruit extracts that were obtained using NADESs. The examination of NADESs revealed that the hydrogen bond acceptors and donors had a substantial impact on the viscosity, pH, density, and chemical structure of the NADESs. The resulting extracts demonstrated the highest recovery rates, resulting in the greatest total phenolic content (35.05 ± 0.15 mg GAE/100 g) and total anthocyanin content (17.22 ± 0.296 mg CGE/100 g). This extract from the <i>Prunus domestica</i> fruit, which contains anthocyanin, has anti-sickling effects on sickle cell erythrocytes. The Emmel test was used to assess the in vitro anti-sickling effects of the NADES extract. The percent sickling of negative control was found to be 40.88%. And it was discovered that the NADES extract of CHCL: ETHGly was 14.71% at an effective concentration of 0.2% (<i>v/v</i>), and the NADES extract of CHCl: GLY was discovered to be 20.94% at an effective concentration of 0.2% (<i>v/v</i>). The phenolic (anthocyanin) impact on percent sickling revealed a significant difference between the control and test.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 10","pages":"1432 - 1445"},"PeriodicalIF":2.6,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141608752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Conventional and Supercritical Fluid Extraction of Lycopene Rich Extract from Heiyai (Elaeagnus latifolia L.) 传统萃取和超临界流体萃取黑叶树番茄红素提取物的优化研究

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-07-02 DOI: 10.1007/s12161-024-02649-y

Nongmaithem Sophia Devi, Bikashchandra Mushahary, Nishant Racchaya Swami Hulle

{"title":"Optimization of Conventional and Supercritical Fluid Extraction of Lycopene Rich Extract from Heiyai (Elaeagnus latifolia L.)","authors":"Nongmaithem Sophia Devi, Bikashchandra Mushahary, Nishant Racchaya Swami Hulle","doi":"10.1007/s12161-024-02649-y","DOIUrl":"10.1007/s12161-024-02649-y","url":null,"abstract":"<div><p>Heiyai (<i>Elaeagnus latifolia</i> L.) is an underutilized semi-wild berry which is found in Manipur during the mid of March and April. In the present study, Heiyai fruit was explored for the extraction of lycopene using supercritical fluid extraction (SFE) using CO<sub>2</sub> and the conventional solvent extraction (CSE) method. An experimental design based on response surface methodology was used for SFE with independent variables (pressure, temperature, and time) and CSE (temperature, time, and solid-to-liquid ratio) to investigate the effect on the extraction yield (%) and lycopene content (mg/100 g) from Heiyai. An empirical model was developed for correlating independent and response variable with a good fit which was obtained. The optimum conditions for SFE were pressure (20 MPa), extraction time (90 min), and temperature (40 °C) resulting into extraction yield of 3.35% and 69.78 mg/100 g of lycopene content. The optimum conditions for CSE were temperature (30 °C), time (15 min), and solid-to-liquid ratio (70 g/mL) resulting into extraction yield of 1.318% and lycopene content of 29.983 mg/100 g. Furthermore, lycopene was isolated and purified using the column chromatography method, and its chemical structure was studied using FT-IR and a visible spectrophotometer.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 10","pages":"1421 - 1431"},"PeriodicalIF":2.6,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141531914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Budget-Friendly Approach to Oxidative Stability Index Measurement 经济实惠的氧化稳定性指数测量方法

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-07-01 DOI: 10.1007/s12161-024-02647-0

Leandro Herculano da Silva, Najla Abou Ghauoche Ahmad, Alex Lemes Guedes, Alex Sanches Torquato, Paulo Rodrigo Stival Bittencourt, Eliane Colla, Vitor Santaella Zanuto, Elton de Lima Savi

{"title":"A Budget-Friendly Approach to Oxidative Stability Index Measurement","authors":"Leandro Herculano da Silva, Najla Abou Ghauoche Ahmad, Alex Lemes Guedes, Alex Sanches Torquato, Paulo Rodrigo Stival Bittencourt, Eliane Colla, Vitor Santaella Zanuto, Elton de Lima Savi","doi":"10.1007/s12161-024-02647-0","DOIUrl":"10.1007/s12161-024-02647-0","url":null,"abstract":"<p>The oxidative stability index is one of the most used and reliable parameters to determine the quality of vegetable oil, biodiesel, and various foods. However, the leading commercially available equipment has a considerably high cost. Nevertheless, block digesters are more financially accessible and are present in most food analysis laboratories. Thus, the proposal of this study allowed us to obtain results very similar to those provided by Rancimat, based on the association between a digester block and a conductivity sensor, assisted by a low-cost and high-efficiency microcontroller, added to a simple graphical interface. Comparing the results between both techniques, it was possible to determine the oxidative stability index with reasonable equivalence and its variation as a function of temperature with a deviation of less than 10% for olive oil. This work helps disseminate oxidative stability analysis and contributes to institutions, researchers, and teachers with limited access to financial resources.</p>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 10","pages":"1411 - 1420"},"PeriodicalIF":2.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141529019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized Eco-friendly Sample Preparation Methods for Determining Major and Minor Elements in Cheeses by ICP OES 利用 ICP OES 测定奶酪中主要和次要元素的优化环保型样品制备方法

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-06-28 DOI: 10.1007/s12161-024-02648-z

Mariana Martins Melo, Fernanda Nunes Ferreira, Aderval S. Luna, Marta Antunes Pereira Langone, Jefferson Santos de Gois

{"title":"Optimized Eco-friendly Sample Preparation Methods for Determining Major and Minor Elements in Cheeses by ICP OES","authors":"Mariana Martins Melo, Fernanda Nunes Ferreira, Aderval S. Luna, Marta Antunes Pereira Langone, Jefferson Santos de Gois","doi":"10.1007/s12161-024-02648-z","DOIUrl":"10.1007/s12161-024-02648-z","url":null,"abstract":"<div><p>This work describes the optimization and validation of sample preparation methods for determining major and minor elements in artisanal Brazilian cheese samples by inductively coupled plasma optical emission spectrometry. Three sample preparation methods were optimized and compared: microwave-assisted digestion, ultrasound-assisted extraction, and alkaline solubilization using tetramethylammonium hydroxide. A central composite design was used for the optimization using the R software. The optimal experimental conditions were achieved at 1.58 mL of HNO<sub>3</sub> 14 mol L<sup>−1</sup> and 0.570 g of sample mass for the microwave-assisted digestion (capable of determining Ca, Fe, K, Mg, Na, and P); 3.4 mol L<sup>−1</sup> of HNO<sub>3</sub>, 0.91 mol L<sup>−1</sup> of HCl, and 2.5 min for the ultrasound-assisted extraction (capable of determining Ca, K, Na, and Zn); and 21.8% (w/w) of tetramethylammonium hydroxide, 50 °C, and 103 min for the AS method (capable of determining K, Na, and Zn). The optimized methods were validated and applied for the analysis of four kinds of artisanal Brazilian cheeses and compared with the dry-ashing method, using a paired <i>t</i>-test, where the means agreed with the dry-ashing method at a 95% confidence interval. </p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 10","pages":"1402 - 1410"},"PeriodicalIF":2.6,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Fluorescence cross-priming Amplification Based on Universal Molecular Beacon for Rapid and Specific Detection of Salmonella enterica in food Samples 基于通用分子信标的新型荧光交叉引物扩增技术，用于快速、特异地检测食品样本中的肠炎沙门氏菌

IF 2.6 3区农林科学

Food Analytical Methods Pub Date : 2024-06-20 DOI: 10.1007/s12161-024-02638-1

Wenfu Hou, Juan Du, Tingting Liu, Wensi Wang, Youwei Ai, Min Zhou, Hongxun Wang, Zhuo Wang

{"title":"A Novel Fluorescence cross-priming Amplification Based on Universal Molecular Beacon for Rapid and Specific Detection of Salmonella enterica in food Samples","authors":"Wenfu Hou, Juan Du, Tingting Liu, Wensi Wang, Youwei Ai, Min Zhou, Hongxun Wang, Zhuo Wang","doi":"10.1007/s12161-024-02638-1","DOIUrl":"10.1007/s12161-024-02638-1","url":null,"abstract":"<div><p>A methodology with rapidity and specificity is of great significance for the effective control and management of outbreaks caused by <i>Salmonella enterica</i> as it has presented an obvious threat to food safety and public health worldwide. In this study, we aimed to develop a tube-closed and real time cross-priming amplification assay for the rapid and sensitive detection of <i>S.enterica</i> and decrease the possibility of detecting false-positive signals derived from aerosol contamination or non-specific amplification caused by traditional CPA assay. The reaction system was optimized and the results showed that molecular beacon CPA (MB-CPA) method was highly specific for detection of <i>S. enterica</i>. The sensitivity of established assay was found to be 10 CFU/mL, 100 CFU/25 g, 10 CFU/25 g in pure culture, chicken sample without and with 6 h enrichment, respectively. And the sensitivity of MB-CPA was 10 times higher than that of real-time PCR. An application of MB-CPA assay was conducted with 78 naturally contaminated food samples to test its practicality. After an enrichment step at 37℃ for 6 h, the results showed 100% sensitivity and 100% specificity compared with standard culture-based method. Considering its rapidity, user-friendliness, cost-effectiveness, this MB-CPA assay will aid in the broader application in food industry for the detection of <i>S. enterica</i> in small or resource-limited food testing laboratories.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 10","pages":"1394 - 1401"},"PeriodicalIF":2.6,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0