Jingxuan Zeng, Haoyue Zheng, Rifeng He, Dan Zhou, Xiaoyan Peng, Nan Mo, Zhonghai Mo, Xue Zhou, Fahuan Ge
{"title":"一种具有优良功能特性的核桃分离蛋白的超临界CO2脱脂- ph多级调节提取新方法","authors":"Jingxuan Zeng, Haoyue Zheng, Rifeng He, Dan Zhou, Xiaoyan Peng, Nan Mo, Zhonghai Mo, Xue Zhou, Fahuan Ge","doi":"10.1007/s12161-025-02777-z","DOIUrl":null,"url":null,"abstract":"<div><p>In this paper, we proposed a new supercritical CO<sub>2</sub> defatting—pH multistage regulation extraction method (SD-PMRE) of high water soluble walnut protein isolate (HWPI), by investigating the effects of PMRE, three defatting methods, and walnut seed coat on the functional properties of HWPI. The results showed that HWPI had high yield of 48.56% and high protein content of 87.58%. More importantly, it had a solubility of 91.58%, significantly higher than walnut protein isolate (WPI) with the highest solubility. Meanwhile, other functional properties of HWPI were greatly improved, with emulsification capacity and stability of 118.07 m<sup>2</sup>/g and 91.03%, and foaming capacity and stability of 129.17% and 89.29%, respectively. Through the SD-PMRE, the problem of WPI’s poor functional properties such as solubility was solved, avoiding the shortcomings brought by modification methods and expanding the application of walnut protein in the fields of food and medicine.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"18 6","pages":"1052 - 1065"},"PeriodicalIF":3.0000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A New Supercritical CO2 Defatting—pH Multistage Regulation Extraction Method of Walnut Protein Isolate With Excellent Functional Properties\",\"authors\":\"Jingxuan Zeng, Haoyue Zheng, Rifeng He, Dan Zhou, Xiaoyan Peng, Nan Mo, Zhonghai Mo, Xue Zhou, Fahuan Ge\",\"doi\":\"10.1007/s12161-025-02777-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this paper, we proposed a new supercritical CO<sub>2</sub> defatting—pH multistage regulation extraction method (SD-PMRE) of high water soluble walnut protein isolate (HWPI), by investigating the effects of PMRE, three defatting methods, and walnut seed coat on the functional properties of HWPI. The results showed that HWPI had high yield of 48.56% and high protein content of 87.58%. More importantly, it had a solubility of 91.58%, significantly higher than walnut protein isolate (WPI) with the highest solubility. Meanwhile, other functional properties of HWPI were greatly improved, with emulsification capacity and stability of 118.07 m<sup>2</sup>/g and 91.03%, and foaming capacity and stability of 129.17% and 89.29%, respectively. Through the SD-PMRE, the problem of WPI’s poor functional properties such as solubility was solved, avoiding the shortcomings brought by modification methods and expanding the application of walnut protein in the fields of food and medicine.</p></div>\",\"PeriodicalId\":561,\"journal\":{\"name\":\"Food Analytical Methods\",\"volume\":\"18 6\",\"pages\":\"1052 - 1065\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-03-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Analytical Methods\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s12161-025-02777-z\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Analytical Methods","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s12161-025-02777-z","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
A New Supercritical CO2 Defatting—pH Multistage Regulation Extraction Method of Walnut Protein Isolate With Excellent Functional Properties
In this paper, we proposed a new supercritical CO2 defatting—pH multistage regulation extraction method (SD-PMRE) of high water soluble walnut protein isolate (HWPI), by investigating the effects of PMRE, three defatting methods, and walnut seed coat on the functional properties of HWPI. The results showed that HWPI had high yield of 48.56% and high protein content of 87.58%. More importantly, it had a solubility of 91.58%, significantly higher than walnut protein isolate (WPI) with the highest solubility. Meanwhile, other functional properties of HWPI were greatly improved, with emulsification capacity and stability of 118.07 m2/g and 91.03%, and foaming capacity and stability of 129.17% and 89.29%, respectively. Through the SD-PMRE, the problem of WPI’s poor functional properties such as solubility was solved, avoiding the shortcomings brought by modification methods and expanding the application of walnut protein in the fields of food and medicine.
期刊介绍:
Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.