Danilo César Mota Martins DDS, MSc, Maurício Gonçalves da Costa Sousa MSc, PhD, Poliana Amanda Oliveira Silva DDS, MSc, PhD, Lana Ribeiro Aguiar MSc, PhD, Rosângela Vieira de Andrade MSc, PhD, Amandda Évellin Silva-Carvalho MSc, PhD, Felipe Saldanha-Araújo MSc, PhD, Octávio Luiz Franco MSc, PhD, Taia Maria Berto Rezende DDS, MSc, PhD
{"title":"Association between host defence peptide IDR-1002 and ciprofloxacin: Effects on human dental pulp cells","authors":"Danilo César Mota Martins DDS, MSc, Maurício Gonçalves da Costa Sousa MSc, PhD, Poliana Amanda Oliveira Silva DDS, MSc, PhD, Lana Ribeiro Aguiar MSc, PhD, Rosângela Vieira de Andrade MSc, PhD, Amandda Évellin Silva-Carvalho MSc, PhD, Felipe Saldanha-Araújo MSc, PhD, Octávio Luiz Franco MSc, PhD, Taia Maria Berto Rezende DDS, MSc, PhD","doi":"10.1111/aej.12866","DOIUrl":"10.1111/aej.12866","url":null,"abstract":"<p>To evaluate the effects of the association of host defence peptide IDR-1002 and ciprofloxacin on human dental pulp cells (hDPSCs). hDPSCs were stimulated with ciprofloxacin and IDR-1002. Cell viability (by MTT assay), migration capacity (by scratch assay), production of inflammatory and anti-inflammatory mediators by hDPSCs (RT-PCR) and osteogenic differentiation (alizarin red staining) were evaluated. Phenotypic profile of hDPSCs demonstrated 97% for positive marked mesenchymal stem cell. Increased pulp cell migration and proliferation were observed after 24 and 48 h of exposure to IDR-1002 with ciprofloxacin. Mineral matrix formation by hDPSCs was observed of the association while its reduction was observed in the presence of peptide. After 24 h, the association between ciprofloxacin and IDR-1002 significantly downregulated <i>TNFRSF-1</i>, <i>IL-1β</i>, <i>IL-8</i>, <i>IL-6</i> and <i>IL-10</i> gene expression (<i>p</i> ≤ 0.0001). The association between the IDR-1002 and ciprofloxacin showed favourable immunomodulatory potential, emerging as a promising option for pulp revascularisation processes.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"547-558"},"PeriodicalIF":1.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sıla Nur Usta DDS, Cangül Keskin DDS, PhD, Burcu Pirimoğlu DDS, Abdurrahman Aksoy PhD
{"title":"Histological response of inflamed pulp to hydraulic calcium silicate cements in direct pulp capping: Systematic review of pulpitis models","authors":"Sıla Nur Usta DDS, Cangül Keskin DDS, PhD, Burcu Pirimoğlu DDS, Abdurrahman Aksoy PhD","doi":"10.1111/aej.12873","DOIUrl":"10.1111/aej.12873","url":null,"abstract":"<p>This systematic review aimed to compare the histological response of inflamed pulpodentinal complex to the hydraulic calcium silicate cements in experimental animal models of pulpitis. Articles that evaluated the histological response of inflamed pulp to mineral trioxide aggregate (MTA) in comparison with other restorative materials were selected and analysed in detail. The risk of bias assessment was conducted using SYRCLE's RoB tool. The GRADEpro tool was used to determine the overall quality of evidence. Out of the 2947 retrieved articles from databases, five articles fulfilled the inclusion criteria. MTA induced significantly more hard tissue formation compared to calcium hydroxide. The use of pulp-capping material containing fluocinolone acetonide and ASP/PLGA-ASP/ACP/PLLA-PLGA composite membrane was comparable. This systematic review could not demonstrate enhanced efficiency of capping materials compared to MTA. Future well-conducted animal studies are warranted for demonstrating the hard tissue formation abilities of pulp-capping materials with convenient inflammatory conditions.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"729-741"},"PeriodicalIF":1.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cone-beam computed tomography-based descriptive classification for transverse root fracture","authors":"Aakriti Saini MDS, Sidhartha Sharma MDS, Vijay Kumar MDS, Amrita Chawla MDS, Shalini Gupta MDS, Bill Kahler PhD, Ajay Logani MDS","doi":"10.1111/aej.12867","DOIUrl":"10.1111/aej.12867","url":null,"abstract":"<p>Transverse root fracture (TRF) is classified as a cervical, middle or apical third root fracture on a periapical radiograph. The International Association of Dental Traumatology (IADT) suggests that cone-beam computed tomography (CBCT) should be considered, when conventional radiographs provide ‘insufficient’ information for diagnosis and treatment planning. Considering that CBCT can divulge additional information, it would be beneficial to have a clinically pertinent three-dimensional classification for TRF. The proposed alphanumeric classification includes the traumatised tooth number, describes the number of TRF, the facial and lingual location of each fracture line on the anatomic root, and its position relative to the crest of the alveolar bone. Further, diastasis, displacement of the coronal fragment and status of the alveolar bone at the site of TRF are also documented. This comprehensive classification system would provide a standard format for reporting, aid in referral communication and can be applied for future outcome studies on TRF.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"718-728"},"PeriodicalIF":1.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141473003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Confocal laser scanning evaluation of the influence of ledges on root canal disinfection","authors":"Elio Berutti MD, DDS, Damiano Pasqualini DDS, Nicola Scotti DDS, PhD, Narcisa Mandras PhD, Janira Roana PhD, Giorgia Carpegna DDS, PhD, Pietro Palopoli DDS, PhD, Mario Alovisi DDS, PhD","doi":"10.1111/aej.12871","DOIUrl":"10.1111/aej.12871","url":null,"abstract":"<p>The aim was to analyse the influence of an apical ledge on root canal disinfection. Forty-four single-rooted teeth were micro-CT scanned and inoculated with <i>Enterococcus faecalis</i>. In Group S shaping was performed with ProTaper Next (PTN) up to X3 at working length (WL). In Group L an apical ledge was created with K-Files #40 and shaping completed up to PTN X3. NaOCl 5% and EDTA 10% irrigant solutions were alternated. Confocal laser scanning microscope (CLSM) and viability staining were used to analyse the proportions of dead (red) and live (green) bacteria and penetration ability inside dentinal tubules. Data were analysed with the Mann–Whitney test with Bonferroni correction (<i>p</i> < 0.05). In Group L the amount of red fluorescence resulted significantly lower, and penetration ability was decreased in the apical and middle portion (<i>p</i> < 0.05). The presence of an apical ledge may negatively influence the disinfection both in the apical and middle third.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"580-586"},"PeriodicalIF":1.3,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141473004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anthony P. Martin MDSc, FRACDS, Stephen C. Harlamb MDSc, MHLaw, MRACDS, FIADT, William N. Ha PhD, DClinDent
{"title":"ASE Position Statement: Dental Dam in Endodontics","authors":"Anthony P. Martin MDSc, FRACDS, Stephen C. Harlamb MDSc, MHLaw, MRACDS, FIADT, William N. Ha PhD, DClinDent","doi":"10.1111/aej.12849","DOIUrl":"10.1111/aej.12849","url":null,"abstract":"","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 2","pages":"215-217"},"PeriodicalIF":1.3,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141460981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eileen Yi Ning Lee BDS, Zi Yee Sua BDS, Shekhar Bhatia BDS, MDS, MFDS RCS (Glas), MFDS RCS (Edin), Shivani Kohli BDS, MDS, MFDS RCS (Glas), MFDS RCS (Edin), Giampiero Rossi-Fedele DDS, MClinDent, PhD, Esma J. Doğramacı BDS, MSc, PhD, Venkateshbabu Nagendrababu BDS, MDS, FDS RCPS (Glasg), PhD
{"title":"Oral health-related quality of life and perceptions of patients following primary root canal treatment or non-surgical retreatment","authors":"Eileen Yi Ning Lee BDS, Zi Yee Sua BDS, Shekhar Bhatia BDS, MDS, MFDS RCS (Glas), MFDS RCS (Edin), Shivani Kohli BDS, MDS, MFDS RCS (Glas), MFDS RCS (Edin), Giampiero Rossi-Fedele DDS, MClinDent, PhD, Esma J. Doğramacı BDS, MSc, PhD, Venkateshbabu Nagendrababu BDS, MDS, FDS RCPS (Glasg), PhD","doi":"10.1111/aej.12868","DOIUrl":"10.1111/aej.12868","url":null,"abstract":"<p>This retrospective cohort study compared the effect of primary root canal treatment (RCT) with root canal retreatment (Re-RCT) on patient-reported outcomes in Kuala Lumpur, Malaysia. Forty randomly selected adults participated (RCT <i>n</i> = 20; Re-RCT <i>n</i> = 20). The impact their dentition had on the Oral Health Impact Profile-14 (OHIP-14) was assessed by calculating the <i>prevalence of oral health impact</i>, and the <i>severity score.</i> Focus group discussions using a semi-structured guide were arranged through an online meeting platform. Qualitative content analysis identified common themes, and relevant quotes gathered. The impact on OHIP-14 was limited for both RCT and Re-RCT groups with no significant differences in the <i>prevalence of oral health impact</i>. Significant differences were found for functional limitation (RCT higher) and psychological discomfort (Re-RCT higher). Common themes from the discussions include the importance of retaining teeth, the significance of effective communication between clinicians and patients and that the respondents were satisfied with the treatment.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"559-570"},"PeriodicalIF":1.3,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141460984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kai-Yun Tso DDS, Yu-Hsueh Wu PhD, Yu-We Tsai DDS, Yu-Sheng Lin PhD, Wei-Chih Chiu MS
{"title":"Endodontic apical surgery with novel endoscope: Three-case series","authors":"Kai-Yun Tso DDS, Yu-Hsueh Wu PhD, Yu-We Tsai DDS, Yu-Sheng Lin PhD, Wei-Chih Chiu MS","doi":"10.1111/aej.12869","DOIUrl":"10.1111/aej.12869","url":null,"abstract":"<p>Successful apical surgery relies on effective magnification and illumination. In the field of endodontics, the microscope has emerged as the predominant tool for meeting these requirements. The rigid endoscope is also a valuable instrument in apical surgery. This study introduces three cases demonstrating the application of endoscope technology in endodontic apical surgery. The first case employs a soft endoscope for treating an anterior tooth with apical periodontitis, the second integrates an endoscope with new attachments for a premolar, and the third combines an endoscope, attachments and navigation for the lower first molar surgery. It revealed that endoscopes offer certain advantages that are not achievable with microscope-assisted surgery, these cases had a great outcome. In the future, a broader application of endoscopic technology in various procedures is anticipated.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"674-686"},"PeriodicalIF":1.3,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141460982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}