Gustavo De-Deus PhD, Fabio Nakao Arashiro PhD, Erick Miranda Souza PhD, Emmanuel João Nogueira Leal Silva PhD, Tiago de Mello Guimarães MSc, Ana Carolina de Carvalho Maciel PhD, Diogo da Silva Oliveira PhD, Felipe Gonçalves Belladonna PhD, Ricardo Tadeu Lopes PhD, Marco Aurélio Versiani PhD
{"title":"The impact of long-term storage conditions on the development of experimental dentinal microcracks","authors":"Gustavo De-Deus PhD, Fabio Nakao Arashiro PhD, Erick Miranda Souza PhD, Emmanuel João Nogueira Leal Silva PhD, Tiago de Mello Guimarães MSc, Ana Carolina de Carvalho Maciel PhD, Diogo da Silva Oliveira PhD, Felipe Gonçalves Belladonna PhD, Ricardo Tadeu Lopes PhD, Marco Aurélio Versiani PhD","doi":"10.1111/aej.12806","DOIUrl":"10.1111/aej.12806","url":null,"abstract":"<p>This study evaluated the impact of long-term storage conditions (medium and time) on the development of experimental dentinal microcracks through micro-computed tomography. Sixty freshly extracted premolars were stored in formalin, water, or dry conditions (<i>n</i> = 20) and scanned after 72 h, 30 days, 6 months and 3 years of extraction. The effect of the storage medium and time on the occurrence of dentinal defects was statistically evaluated. A total of 211 000 images were screened revealing the existence of 11 519 slices with dentinal defects. Dry conditions significantly contributed to the development of new defects in all time points. During the 3-year follow-up period, no new defects were detected in the teeth that were stored in water and, in a single tooth, in the formalin group, after 6 months of storage.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 1","pages":"60-68"},"PeriodicalIF":1.6,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50159444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Syringe irrigation in confluent canals: A sequential computational fluid dynamics assessment","authors":"Mário Rito Pereira DDS, MSc, Goncalo Silva PhD, Viriato Semiao MSc, PhD, Jorge N. R. Martins DDS, MSc, PhD, Vania Silverio PhD, Paula Pascoal-Faria PhD, Isabel Duarte MSc, PhD, Nuno Alves PhD, António Ginjeira MD, DMD, PhD","doi":"10.1111/aej.12805","DOIUrl":"10.1111/aej.12805","url":null,"abstract":"<p>This study aims to assess the influence of root canal preparation, irrigation needle design and its placement depth in the irrigation flow of confluent canals during syringe irrigation. A mandibular molar presenting two confluent canals in its mesial root was sequentially prepared and scanned by micro-computed tomography after mechanical preparation up to ProTaper Next system sizes X2 (25/.06v), X3 (30/.07v) and X4 (40/.06v). In each of the root canal preparation models, a side-vented and an open-ended needle at 5, 3 and 2 mm from the working length were included, and irrigation flow was assessed by a validated computational fluid dynamics model. The results revealed that the irrigant flowed out of the confluent canals mainly through the canal that did not have the needle. Apical penetration and renewal of the irrigant were most efficiently achieved with the use of a 30G open-ended needle and a 30/.07v preparation.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 1","pages":"40-51"},"PeriodicalIF":1.6,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raghda A. K. Almazedi BDS, MSc, Iman M. Al-Zaka BDS, MSc
{"title":"Influence of two kinematics on canal transportation and centering ability of WaveOne Gold and One Curve files","authors":"Raghda A. K. Almazedi BDS, MSc, Iman M. Al-Zaka BDS, MSc","doi":"10.1111/aej.12804","DOIUrl":"10.1111/aej.12804","url":null,"abstract":"<p>This research examines canal transportation and centering ability of single-file systems when operated in continuous rotation or reciprocation. Sixty J-shaped resin blocks were used. The specimens were divided into two main groups (<i>n</i> = 30). Then, based on the motion used to operate the instruments, each group was subdivided into two subgroups (<i>n</i> = 15). Group A1: One Curve/rotation, Group A2: One Curve/clockwise reciprocation, Group B1: WaveOne Gold/Reciprocation, Group B2: WaveOne Gold/counter-clockwise rotation. Pre- and post-instrumentation images were obtained using digital microscope. Canal transportation was measured at five levels. A non-significant difference (<i>p</i> > 0.05) resulted between two motions at all levels. However, group A1 showed significantly less transportation at levels 3 and 4 (<i>p</i> ≤ 0.05), also more centered preparation at level 4 (<i>p</i> ≤ 0.05) than group B1. Within the limitations of this study, the apical transportation and centering ability of single-file systems are unaffected by different kinematics.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 1","pages":"24-39"},"PeriodicalIF":1.6,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarah Samir Abouelenien MSc, PhD, Olfat Gamil Shaker MSc, PhD, Dina Ahmed Morsy MSc, PhD
{"title":"Cytokine expression of IL-8 before and after root canal treatment in patients with symptomatic apical periodontitis: A clinical trial","authors":"Sarah Samir Abouelenien MSc, PhD, Olfat Gamil Shaker MSc, PhD, Dina Ahmed Morsy MSc, PhD","doi":"10.1111/aej.12803","DOIUrl":"10.1111/aej.12803","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>This study's objective was to compare the cytokine expression of IL-8 in periapical tissues of single-rooted teeth with symptomatic apical periodontitis (SAP) before and after root canal treatments. As well as, comparing IL-8 levels in peri-apical tissues between vital and necrotic teeth with SAP.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>Thirty-six patients were allocated according to their pulp status into two experimental groups (<i>n</i> = 18) receiving the same treatment protocol; group 1: Vital pulps with SAP, and group 2: non-vital pulps with SAP. Conventional endodontic treatment was done on two visits; isolation and disinfection of the operative field were undertaken, and two-stage access cavity preparation was implemented. The first pre-instrumentation peri-apical sample (S1) was collected prior to cleaning and shaping procedures. A 2.5% NaOCl irrigation was used to thoroughly irrigate the canal after performing root canal preparation utilising the ProTaper Next (PTN) rotary system. After 1 week, the second post-instrumentation peri-apical sample (S2) was collected. Using an ELISA kit, the quantity of IL-8 was evaluated following the collection of all samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In all pre-instrumentation samples, IL-8 was detected (100%). The level of IL-8 expression was significantly decreased from the S1 to S2 of all samples (<i>p</i> < 0.001). The intra-group comparison showed a statistically significant reduction in the level of IL-8 expression between S1 and S2 in both vital and non-vital groups where <i>p</i> < 0.001* in both groups. The inter-group comparison of levels of IL-8 expression (vital and non-vital) revealed a significant difference between both groups regarding the pretreatment sample with the higher levels of IL-8 shown in the non-vital group (<i>p</i> < 0.001). While in the post-treatment sample, both groups showed a significant reduction in the level of IL-8 expression but the difference between them was not statistically significant (<i>p</i> = 0.226).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Root canal instrumentation seems to be efficient in decreasing the levels of anti-inflammatory cytokines, namely IL-8. Further research should clarify how intra-canal medicaments affect inflammatory mediator levels.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 1","pages":"15-23"},"PeriodicalIF":1.6,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41221024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gulbin Sehitoglu DDS, Fatih Cakici DDS, PhD, Saniye Soylemez PhD, Cagatay Dengiz DSc
{"title":"Evaluation of the effect of graphene oxide-based nanocomposites on smear layer by a scanning electron microscope: Laboratory investigation","authors":"Gulbin Sehitoglu DDS, Fatih Cakici DDS, PhD, Saniye Soylemez PhD, Cagatay Dengiz DSc","doi":"10.1111/aej.12802","DOIUrl":"10.1111/aej.12802","url":null,"abstract":"<p>This study is to examine the effect of graphene oxide (GO) and GO-based nanocomposites (GO_EDTA, GO_CHIT) on the smear layer by scanning electron microscopy (SEM). Sixty human single-rooted anterior teeth were used. Samples were randomly categorised into six groups. Distilled water in group 1, 17% EDTA in group 2, chitosan (CHIT) solution in group 3, GO solution in group 4, GO_EDTA solution in group 5 and GO_CHIT in group 6 were used as irrigation solutions. Photomicrographs were acquired from the coronal, middle and apical parts of the samples by SEM. Statistical analyses were performed using Kruskal-Wallis, Mann–Whitney U and Wilcoxon tests. There was a statistically significant difference among the groups (<i>p</i> < 0.003). Adding GO to CHIT and EDTA did not affect removing the smear layer in all segments (<i>p</i> < 0.003). Incorporating GO into EDTA and CHIT solutions did not change their interaction with the smear layer.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 1","pages":"3-14"},"PeriodicalIF":1.6,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41155169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Endodontic management of an aberrant germinated composite odontome: A case report","authors":"Ankit Arora BDS, MDS, Kavina Desai BDS, Sonali Kapoor BDS, MDS, Seema Gajera BDS","doi":"10.1111/aej.12801","DOIUrl":"10.1111/aej.12801","url":null,"abstract":"<p>To describe the endodontic treatment of a germinated composite odontome with atypical internal anatomy. A 35-year-old male presented with pain in the left mandibular second molar region. Intraoral examination and Cone-Beam Computed Tomography (CBCT) revealed a rare case of a germinated composite odontome with a 3-2-1 Vertucci configuration in the mesial root. Occlusal morphology and mesial root configuration required modifications in methods of isolation and access cavity preparation. Contemporary tools like magnification, ultrasonics and controlled memory rotary files were essential for executing the treatment plan. The tooth was functional at 18 months follow up and radiographs revealed no abnormality.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"49 3","pages":"684-689"},"PeriodicalIF":1.6,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41156455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Finite element stress analysis of dental cement application on endocrown and onlay restoration","authors":"Satrio Wicaksono PhD, Wandi Prasetia MSc, Anna Muryani Sp KG, Subsp KE(K), Tatacipta Dirgantara PhD, Andi Isra Mahyuddin PhD","doi":"10.1111/aej.12800","DOIUrl":"10.1111/aej.12800","url":null,"abstract":"<p>The aim of the study was to evaluate the influence of resin cement material types on tooth with endocrown and onlay restorations. The first molar was scanned using Micro-CT and underwent a modelling process to obtain the 3D model for computational simulation. Eight models were simulated in the current study with two loading conditions (720N vertical load and 200N oblique load), two types of restoration (onlay and endocrown restorations), and two resin cement variants (dual-cure resin cement and light-cure resin cement). The tooth with onlay restoration showed a significant stress reduction (up to 70%) when using light-cure resin cement compared to dual-cure resin cement. In contrast, types of cement did not affect stress distribution in the tooth with endocrown restoration. The current study found that dual-cure resin cement was preferable in Endocrown and Onlay restorations, due to dual-cure resin cement provided better bond strength compared to light-cure resin cement.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"49 3","pages":"665-674"},"PeriodicalIF":1.6,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41153676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sevda Durust Baris DMD, Ali Turkyilmaz DMD, PhD, Mehmet Kursat Derici MD
{"title":"Effects of Nd: YAG laser on tumour necrosis factor-alpha, interleukin-1beta and interferon-gamma levels in teeth with apical periodontitis: A clinical study","authors":"Sevda Durust Baris DMD, Ali Turkyilmaz DMD, PhD, Mehmet Kursat Derici MD","doi":"10.1111/aej.12799","DOIUrl":"10.1111/aej.12799","url":null,"abstract":"This study aimed to evaluate the levels of tumour necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and interferon-gamma (IFN-γ) with asymptomatic apical periodontitis (AP). A total of 60 participants were randomly divided into two groups: the conventional irrigation (control) and the Nd: YAG laser irradiation. The interstitial fluids were obtained after root canal cleaning (day 0) and 1 week later (day 7). The TNF-α, IL-1β and IFN-γ levels were assayed using the enzyme-linked immunosorbent assay. The Mann-Whitney U, continuity correction chi-square, Pearson chi-square and Fisher exact tests were used. An increased level of cytokines on day 7 in the control group was observed, without statistically significant differences (p > 0.05). All cytokine levels decreased over time in the laser group. Only the IL-1β level showed a significant difference (p < 0.05). Nd: YAG irradiation has a positive effect on decreasing the proinflammatory cytokine level and may help to control infection in teeth with AP.","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"49 3","pages":"657-664"},"PeriodicalIF":1.6,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41170923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Single nucleotide polymorphisms in inducible nitric oxide synthase gene are not associated with persistent apical periodontitis","authors":"Sandra Regina Santos Meyfarth DDS, MSc, Lívia Azeredo Alves Antunes DDS, MSc, PhD, Jhenyfer da Silva Tavares BSc, Ludmila da Silva Guimarães DDS, MSc, PhD, Erlange Andrade Borges da Silva DDS, MSc, PhD, Flares Baratto-Filho DDS, MSc, PhD, Erika Calvano Küchler DDS, MSc, PhD, Alice Corrêa Silva-Sousa DDS, MSc, PhD, Manoel Damião Sousa-Neto DDS, MSc, PhD, Leonardo Santos Antunes DDS, MSc, PhD","doi":"10.1111/aej.12796","DOIUrl":"10.1111/aej.12796","url":null,"abstract":"<p>The aim of this study was to investigate whether there is an association between inducible in single nucleotide polymorphisms in nitric oxide synthase (rs2297518 and rs2779249) and persistent apical periodontitis. A total of 291 Brazilian subjects were included: 125 with signs/symptoms of persistent apical periodontitis and 166 with root canal-treated teeth exhibiting healthy perirradicular tissues. Endodontically treated patients were followed up after 1 year. The two single nucleotide polymorphisms in nitric oxide synthase were analysed using real-time polymerase chain reaction. Chi-square test and odds ratio with 95% confidence intervals were performed to compare genotype distributions between ‘healed’ and ‘persistent apical periodontitis’ groups (<i>p</i> < 0.05). Logistic regression analysis was used to evaluate SNP–SNP interactions. The allele and genotype distributions for the polymorphisms between the persistent apical periodontitis and healed groups were not statistically significant (<i>p</i> > 0.05). In the logistic regression analysis, the polymorphisms were not associated with persistent apical periodontitis and SNP–SNP interactions.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"49 3","pages":"648-656"},"PeriodicalIF":1.6,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41161631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana I. Soza-Bolaños DDS, Endod, Rubén A. Domínguez-Pérez DDS, MSc, Endod, PhD, José L. Ayala-Herrera DDS, MSc, PhD, Rosa M. Pérez-Serrano PhD, Uriel Soto-Barreras DDS, MSc, PhD, León F. Espinosa-Cristóbal DDS, MSc, PhD, Claudia A. Rivera-Albarrán DDS, MSc, PhD, Guadalupe Zaldívar-Lelo de Larrea MD, MSc, PhD
{"title":"Presence of methanogenic archaea in necrotic root canals of patients with or without type 2 diabetes mellitus","authors":"Ana I. Soza-Bolaños DDS, Endod, Rubén A. Domínguez-Pérez DDS, MSc, Endod, PhD, José L. Ayala-Herrera DDS, MSc, PhD, Rosa M. Pérez-Serrano PhD, Uriel Soto-Barreras DDS, MSc, PhD, León F. Espinosa-Cristóbal DDS, MSc, PhD, Claudia A. Rivera-Albarrán DDS, MSc, PhD, Guadalupe Zaldívar-Lelo de Larrea MD, MSc, PhD","doi":"10.1111/aej.12797","DOIUrl":"10.1111/aej.12797","url":null,"abstract":"<p>Theoretically, a necrotic root canal fulfils all requirements as a niche for methanogens to inhabit. However, their presence in it and its implication in apical periodontitis (AP) is controversial. Therefore, to contribute to ending the controversy, this study aimed to detect and compare methanogens' presence in two distinct niches with supposedly different microenvironments; both were necrotic root canals associated with AP but one from patients with type 2 diabetes mellitus (T2DM) while the other from non-diabetic patients. A clinical examination was performed on 65 T2DM patients and 73 non-diabetic controls. Samples from necrotic root canals were obtained, and methanogens were identified. The presence of methanogens was three times higher (27.6%) in the T2DM group than in non-diabetic patients (8.2%). In addition, methanogens' presence was associated with a higher prevalence of periapical symptoms.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"49 3","pages":"641-647"},"PeriodicalIF":1.6,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aej.12797","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10254230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}