{"title":"Antibiofilm effect of different concentrations of silver nanoparticles combined with calcium hydroxide against Enterococcus faecalis biofilm: An ex vivo study.","authors":"Sumaya Basudan, Alanoud Alqahtani, Fatemah Alrwais, Reem Almeaither, Sayed Auda, Hanan A Balto","doi":"10.1111/aej.12882","DOIUrl":"https://doi.org/10.1111/aej.12882","url":null,"abstract":"<p><p>This study aimed to evaluate the antibiofilm activity of different concentrations of silver nanoparticles (AgNPs) in combination with calcium hydroxide [Ca(OH)<sub>2</sub>] against Enterococcus faecalis biofilm. On an E. faecalis biofilm on dentin discs, the following medicaments were applied for 7 days (n = 13/group): 0.005% AgNPs+Ca(OH)<sub>2</sub>, 0.01% AgNPs+Ca(OH)<sub>2</sub>, 0.02% AgNPs + Ca(OH)<sub>2</sub>, Ca(OH)<sub>2</sub> and saline/control. Specimens were stained with LIVE/DEAD® BacLight™ dye and analysed with confocal laser scanning microscopy. Proportion of dead bacteria was calculated and analysed. There was a significant reduction in E. faecalis biofilm in all medicament groups (43.5%, 49.1%, 69.1%, 48.7%) respectively, compared with control group (2.54%) (p < 0.001). The 0.02% AgNPs + Ca(OH)<sub>2</sub> group demonstrated the most significantly superior antibiofilm effect, with no significant difference between remaining groups. In conclusion, combining 0.02% AgNPs enhanced the antibiofilm effect of Ca(OH)<sub>2</sub> on E. faecalis biofilm compared with lower AgNPs concentrations.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141977338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sukainah A Aldawood, Omar Omar, Dana Almohazey, Amre R Atmeh
{"title":"Reliability of sealer penetration measurement methods and assessment of dentine penetrability after endodontic retreatment: A correlation in vitro study.","authors":"Sukainah A Aldawood, Omar Omar, Dana Almohazey, Amre R Atmeh","doi":"10.1111/aej.12881","DOIUrl":"https://doi.org/10.1111/aej.12881","url":null,"abstract":"<p><p>This study evaluated the reliability of sealer penetration measurement methods used with confocal laser scanning microscopy in correlation with the percentage of residual root filling and examined the effect of residual root-filling material on dentine penetrability after retreatment. Extracted teeth were randomly divided into different groups according to the obturation sealer used (n = 6); BioRoot RCS; MTA Fillapex; Bio-C and AH Plus. Root-filling material was removed before the secondary chemo-mechanical preparation and obturation using fluorescein labelled AH Plus. Multiple micro-computed tomography scans were obtained followed by confocal laser scanning microscopy to measure the penetration of the labelled sealer into the dentinal tubules using four different methods. Measuring sealer penetration into radicular dentine using the penetration percentage method was found to be the most reliable. Dentine penetrability during retreatment did not seem to be affected by the type of residual root-filling material.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"APEC News July 2024: Ongoing process for organisation of APEC2025 Dhaka","authors":"Hyeon-Cheol Kim DDS, PhD","doi":"10.1111/aej.12879","DOIUrl":"10.1111/aej.12879","url":null,"abstract":"","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 2","pages":"188-190"},"PeriodicalIF":1.3,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141774216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taher Al Omari, Amre R Atmeh, Fahda N Algahtani, Alaa Dkmak, Rami Haitham Issa Albanna, Abedelmalek Tabnjh
{"title":"The effect of irrigation solution temperature and novel heat-treated rotary files on apical debris extrusion and canal preparation time.","authors":"Taher Al Omari, Amre R Atmeh, Fahda N Algahtani, Alaa Dkmak, Rami Haitham Issa Albanna, Abedelmalek Tabnjh","doi":"10.1111/aej.12878","DOIUrl":"https://doi.org/10.1111/aej.12878","url":null,"abstract":"<p><p>This study aimed to compare apical debris extrusion and canal preparation time with ProTaper Gold (PTG) and ProTaper Ultimate (PTU) files at different temperatures. Mesio-buccal roots of 60 mandibular first molars were distributed into six groups depending on the file type (PTG, PTU) and irrigation solution temperature (20, 37, 45°C). During instrumentation, extruded debris were collected and weighed to measure the mass in milligrams. The canal preparation time was recorded in seconds. ANOVA followed by Bonferroni post-hoc tests were used for analysis. The amount of debris extrusion was significantly higher in PTU, which was affected by the irrigation solution temperature (p < 0.05). The difference in canal preparation time was not significant between the two file systems, however, it was significant between the different temperatures (p = 0.001). Both file systems had shorter canal preparation times at 20°C. The irrigation solution temperature could influence the debris extrusion and time of canal preparation.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniela Auerbach, Ibrahim Alaugaily, Scott Davis, Adham A Azim
{"title":"A promising approach utilising photothermal energy to disinfect the root canal system: An in vitro investigation.","authors":"Daniela Auerbach, Ibrahim Alaugaily, Scott Davis, Adham A Azim","doi":"10.1111/aej.12872","DOIUrl":"https://doi.org/10.1111/aej.12872","url":null,"abstract":"<p><p>This study aimed to assess root canal disinfection through various irrigation protocols, including a novel photothermal system called 'LEAP'. Mandibular premolars were infected with Enterococcus faecalis and divided into five groups for different treatments: Group 1: standard needle irrigation; Group 2: passive ultrasonic irrigation; Group 3: GentleWave; Group 4: LEAP; and Group 5: Group 1 + Group 4. Microbial counts were measured before (S1) and after disinfection (S2) using colony-forming units (CFU) and confocal laser scanning microscopy (CLSM). Results revealed a significant reduction in bacterial counts for all groups (p < 0.05). While the percentage of dead bacteria near the canal wall (0-50 μm) did not differ significantly, at 50-150 μm, LEAP and SNI + LEAP exhibited significantly higher bacterial reduction than other groups (p < 0.05). The findings indicate that LEAP is comparable to existing irrigation devices in the main root canal and notably superior in tubular disinfection.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"AEJ report for ASE NSW 62nd AGM and 247th General Meeting","authors":"Atieh (Ati) Sadr DDS, MS","doi":"10.1111/aej.12877","DOIUrl":"10.1111/aej.12877","url":null,"abstract":"","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 2","pages":"468-470"},"PeriodicalIF":1.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141587976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Danilo César Mota Martins, Maurício Gonçalves da Costa Sousa, Poliana Amanda Oliveira Silva, Lana Ribeiro Aguiar, Rosângela Vieira de Andrade, Amandda Évellin Silva-Carvalho, Felipe Saldanha-Araújo, Octávio Luiz Franco, Taia Maria Berto Rezende
{"title":"Association between host defence peptide IDR-1002 and ciprofloxacin: Effects on human dental pulp cells.","authors":"Danilo César Mota Martins, Maurício Gonçalves da Costa Sousa, Poliana Amanda Oliveira Silva, Lana Ribeiro Aguiar, Rosângela Vieira de Andrade, Amandda Évellin Silva-Carvalho, Felipe Saldanha-Araújo, Octávio Luiz Franco, Taia Maria Berto Rezende","doi":"10.1111/aej.12866","DOIUrl":"https://doi.org/10.1111/aej.12866","url":null,"abstract":"<p><p>To evaluate the effects of the association of host defence peptide IDR-1002 and ciprofloxacin on human dental pulp cells (hDPSCs). hDPSCs were stimulated with ciprofloxacin and IDR-1002. Cell viability (by MTT assay), migration capacity (by scratch assay), production of inflammatory and anti-inflammatory mediators by hDPSCs (RT-PCR) and osteogenic differentiation (alizarin red staining) were evaluated. Phenotypic profile of hDPSCs demonstrated 97% for positive marked mesenchymal stem cell. Increased pulp cell migration and proliferation were observed after 24 and 48 h of exposure to IDR-1002 with ciprofloxacin. Mineral matrix formation by hDPSCs was observed of the association while its reduction was observed in the presence of peptide. After 24 h, the association between ciprofloxacin and IDR-1002 significantly downregulated TNFRSF-1, IL-1β, IL-8, IL-6 and IL-10 gene expression (p ≤ 0.0001). The association between the IDR-1002 and ciprofloxacin showed favourable immunomodulatory potential, emerging as a promising option for pulp revascularisation processes.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sıla Nur Usta, Cangül Keskin, Burcu Pirimoğlu, Abdurrahman Aksoy
{"title":"Histological response of inflamed pulp to hydraulic calcium silicate cements in direct pulp capping: Systematic review of pulpitis models.","authors":"Sıla Nur Usta, Cangül Keskin, Burcu Pirimoğlu, Abdurrahman Aksoy","doi":"10.1111/aej.12873","DOIUrl":"https://doi.org/10.1111/aej.12873","url":null,"abstract":"<p><p>This systematic review aimed to compare the histological response of inflamed pulpodentinal complex to the hydraulic calcium silicate cements in experimental animal models of pulpitis. Articles that evaluated the histological response of inflamed pulp to mineral trioxide aggregate (MTA) in comparison with other restorative materials were selected and analysed in detail. The risk of bias assessment was conducted using SYRCLE's RoB tool. The GRADEpro tool was used to determine the overall quality of evidence. Out of the 2947 retrieved articles from databases, five articles fulfilled the inclusion criteria. MTA induced significantly more hard tissue formation compared to calcium hydroxide. The use of pulp-capping material containing fluocinolone acetonide and ASP/PLGA-ASP/ACP/PLLA-PLGA composite membrane was comparable. This systematic review could not demonstrate enhanced efficiency of capping materials compared to MTA. Future well-conducted animal studies are warranted for demonstrating the hard tissue formation abilities of pulp-capping materials with convenient inflammatory conditions.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}