Acta Veterinaria-Beograd最新文献

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Purulent Renal Papillitis Due to Streptococcus Infantarius Subsp. Infantarius in a Horse 小儿链球菌所致化脓性肾乳头炎。骑在马上的婴儿
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2022-03-01 DOI: 10.2478/acve-2022-0011
Y. Eroksuz, B. Otlu, Z. Yerlikaya, E. Tanrıverdi, C. A. Incili, B. Karabulut, M. Timurkan, H. Eroksuz
{"title":"Purulent Renal Papillitis Due to Streptococcus Infantarius Subsp. Infantarius in a Horse","authors":"Y. Eroksuz, B. Otlu, Z. Yerlikaya, E. Tanrıverdi, C. A. Incili, B. Karabulut, M. Timurkan, H. Eroksuz","doi":"10.2478/acve-2022-0011","DOIUrl":"https://doi.org/10.2478/acve-2022-0011","url":null,"abstract":"Abstract A 6-year-old, male Arabian crossbred horse was necropsied after a 10 day history of loss of appetite, debility and weight loss. Gross and histologic examination was consistent with purulent papillitis due to Streptococcus infantarius subsp. infantarius. The isolate was sensitive to all the antibiotics tested.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"124 - 130"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49193324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Serum Iron and Ferritin Levels as Inflammatory Markers in Calves with Bovine Respiratory Disease Complex 牛呼吸道疾病复合体犊牛血清铁和铁蛋白水平作为炎症标志物的评价
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2022-03-01 DOI: 10.2478/acve-2022-0005
Ş. Değirmençay, A. Kirbas, H. Aydın, Ömer Aydin, M. Aktas, Ramazan Kaman
{"title":"Evaluation of Serum Iron and Ferritin Levels as Inflammatory Markers in Calves with Bovine Respiratory Disease Complex","authors":"Ş. Değirmençay, A. Kirbas, H. Aydın, Ömer Aydin, M. Aktas, Ramazan Kaman","doi":"10.2478/acve-2022-0005","DOIUrl":"https://doi.org/10.2478/acve-2022-0005","url":null,"abstract":"Abstract Iron and ferritin have been used in human medicine for years to reveal the presence of inflammation. However, studies evaluating these parameters, especially in respiratory system diseases, are quite rare in veterinary medicine. We aimed to test the usability of serum Fe and Fe-related parameters [total iron-binding capacity (TIBC), unsaturated iron-binding capacity (UIBC) and transferrin saturation (TS) levels] as inflammatory and diagnostic biomarkers in calves with bovine respiratory disease complex (BRDC). To mark inflammation, some selected acute-phase proteins including serum ferritin and transferrin levels were measured because of their close relationship with iron metabolism. The material of this study consisted of 15 calves, aged 1-3 months with BRDC (Group I) and 10 healthy calves aged 1-3 months (Group II) based on the presence of respiratory clinical findings. Serum Fe, TIBC and TS levels were low and ferritin levels were high in Group I (P ≤ 0.001). The BRDC group was separated into two subgroups based on PCR results, namely Virus+ (n=9) and Virus- (n=6). The calves in the Virus+ group had significantly lower levels of Fe (P=0.001) and significantly higher values of ferritin (P=0.002), compared to the healthy group. On the basis of inter-group comparison and ROC analysis, we concluded that Fe (primarily), ferritin, TIBC and TS levels can be used as inflammatory biomarkers and possible diagnostic markers in the BRDC as useful, practical, inexpensive substitutes. As a suggestion, these parameters which are believed to play a role in the pathogenesis of the disease, can be used as potential prognostic biomarkers in studies involving treatment.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"59 - 75"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43877743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and Validation of an Improved HPLC-UV Method for the Determination of Tildipirosin in Horse Plasma 高效液相色谱-紫外分光光度法测定马血浆中替地匹罗辛含量的建立与验证
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2022-03-01 DOI: 10.2478/acve-2022-0008
J. S. Galecio, V. Hernandis, E. Escudero, P. Marín
{"title":"Development and Validation of an Improved HPLC-UV Method for the Determination of Tildipirosin in Horse Plasma","authors":"J. S. Galecio, V. Hernandis, E. Escudero, P. Marín","doi":"10.2478/acve-2022-0008","DOIUrl":"https://doi.org/10.2478/acve-2022-0008","url":null,"abstract":"Abstract A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in horse plasma. Plasma samples were extracted with diethyl ether, and after evaporation, tildipirosin was determined by reverse-phase chromatography with an ultraviolet detector set at a wavelength of 289 nm. Tildipirosin was separated on a Zorbax Eclipse XDB-C18 column, 150 x 3.0 mm, 5 μm with gradient chromatographic elution. The retention times were 3.0 min and 6.4 min for tildipirosin and tylosin tartrate, respectively. The total run time was 9 minutes in this method. Calibration curves ranged from 0.1 to 3 μg/mL. The lower limit of detection for plasma was0.035μg/mL, and the lower limit of quantitation was 0.1 μg/mL. Both accuracy and precision were always < 12% exce pt for LLOQ < 20%. Mean recovery was 99.5 %. This procedure can be applied to determine tildipirosin concentrations in plasma and be useful to perform pharmacokinetic studies.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"100 - 110"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49126617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Investigation of the Effects of L-carnitine and magnesium on Oxidative Stress and Cytokines in the Tissue of Experimental diabetic rats L-肉碱和镁对实验性糖尿病大鼠组织氧化应激和细胞因子影响的研究
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2021-12-01 DOI: 10.2478/acve-2021-0039
Meryem Senturk, M. Eren, Z. Sarıca
{"title":"Investigation of the Effects of L-carnitine and magnesium on Oxidative Stress and Cytokines in the Tissue of Experimental diabetic rats","authors":"Meryem Senturk, M. Eren, Z. Sarıca","doi":"10.2478/acve-2021-0039","DOIUrl":"https://doi.org/10.2478/acve-2021-0039","url":null,"abstract":"Abstract The aim of this study was to determine the effects of L-carnitine and magnesium on the levels of tissue malondialdehyde, 8-hydroxy-2’-deoxyguanosine, and cytokines (tumor necrosis factor alpha, interleukin-6) in streptozotocin-induced experimental diabetes in rats. Eighty male Wistar albino rats (200-250 g) were divided into 8 groups with 10 rats in each group. The groups received the following treatments: Control group; 2 ml distilled water (by gavage); Group 2: 50 mg/kg (b.w.) i.p. streptozotocin; Group 3: 125 mg/kg (b.w.) magnesium; Group 4: 300 mg/kg (b.w.) L-carnitine; Group 5: 125 mg/kg (b.w.) magnesium +300 mg/kg (b.w.) L-carnitine; Group 6: 50 mg/kg (b.w.) streptozotocin +125 mg/kg (b.w.) magnesium; Group 7: 50 mg/kg (b.w.) streptozotocin +300 mg/kg (b.w.) L-carnitine and Group 8: 50 mg/kg (b.w.) streptozotocin +125 mg/ kg (b.w.) magnesium+300 mg/kg (b.w.) L-carnitine administered for 4 weeks. Liver and kidney malondialdehyde, 8-hydroxy-2’-deoxyguanosine, tumor necrosis factor alpha and interleukin-6 levels did not change in the magnesium, L-carnitine, and magnesium + L-carnitine groups compared to the control. The highest levels of malondialdehyde, 8-hydroxy-2’-deoxyguanosine, tumor necrosis factor alpha and interleukin-6 were determined only in the group with diabetes (Group 2). Lipid peroxidation, DNA damage, and cytokine levels were significantly reduced in diabetic animals with the administration of magnesium and L-carnitine separately or in combination. Based on the obtained results it can be concluded that magnesium and L-carnitine may have antidiabetic effects, especially in combination.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"71 1","pages":"477 - 489"},"PeriodicalIF":0.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49555359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Multimodal Palliative efficacy of gabapentin and tramadol By Using Two Pain Scoring Systems in Cats Undergoing Ovariohysterectomy 用两种疼痛评分系统比较加巴喷丁和曲马多对卵巢子宫切除术猫的多模式缓解疗效
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2021-12-01 DOI: 10.2478/acve-2021-0035
A. Rabbani, Q. Ullah, O. Naseer, Faizan Haider Gardezi, M. Shahid, Kashif Hussain, Taimoor Saleem, Ahmad Ali, Y. Khan, Abdul Waheed
{"title":"Comparative Multimodal Palliative efficacy of gabapentin and tramadol By Using Two Pain Scoring Systems in Cats Undergoing Ovariohysterectomy","authors":"A. Rabbani, Q. Ullah, O. Naseer, Faizan Haider Gardezi, M. Shahid, Kashif Hussain, Taimoor Saleem, Ahmad Ali, Y. Khan, Abdul Waheed","doi":"10.2478/acve-2021-0035","DOIUrl":"https://doi.org/10.2478/acve-2021-0035","url":null,"abstract":"Abstract The analgesic efficacy of the gabapentin-tramadol combination was compared with meloxicam-tramadol and tramadol perioperative analgesic regimens in cats brought to the clinic for ovariohysterectomy. Thirty adult cats belonging to comparable demographics (age, body weight), were enrolled into a randomized, blinded study after due consent from their owners into four treatment groups. A Gabapentin-Tramadol group (GT-group, n = 10), Meloxicam-Tramadol group (MT-group, n = 10), and a Tramadol group (T-group, n = 10) were formed. Gabapentin capsules at 50 mg were administered orally 2 hours before surgery while the rest received a placebo dose. Tramadol (2 mg/kg, IM) and meloxicam at (0.2 mg/kg, SC) were injected immediately prior to anesthetic premedication. Anesthetic protocol involved premedication with ketamine and xylazine, while anesthesia was induced using propofol. Inhalant isoflurane anesthesia was used to maintain a surgical plane. GT group scored lower on IVAS as well as CPS than MT group, and T group for up to 8 hours after surgery. The mechanical nociceptive threshold remained higher (98±0) for up to 12 hours postoperatively a nd serum cortisol concentrations remained significantly lower during the 24hr period. The addition of gabapentin to the tramadol regimen significantly improved analgesia and mechanical nociceptive threshold than when used on its own.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"71 1","pages":"417 - 434"},"PeriodicalIF":0.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47268864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Key risk factors and impact of African swine fever spreading on pig production in Serbia 非洲猪瘟传播对塞尔维亚养猪生产的主要危险因素和影响
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2021-12-01 DOI: 10.2478/acve-2021-0032
V. Polaček, J. Mirčeta, J. Prodanov-Radulović
{"title":"Key risk factors and impact of African swine fever spreading on pig production in Serbia","authors":"V. Polaček, J. Mirčeta, J. Prodanov-Radulović","doi":"10.2478/acve-2021-0032","DOIUrl":"https://doi.org/10.2478/acve-2021-0032","url":null,"abstract":"Abstract African swine fever (ASF) is a viral disease of domestic pigs and wild boars and currently represents a major threat to the swine industry worldwide. Disease control is impaired by a lack of an effective vaccine and currently, it is dependent on biosecurity measures in pig production, rapid diagnosis, and stamping out of infected herds. Consequently, this swine disease has considerable social-economic significance on national or even regional level. In 2019 for the first time ASF was detected in the domestic swine population (backyards) in the central region of Serbia. From then on, there have been continuous outbreaks of new cases in the population of domestic and wild boars. Considering domestic pig population, in the majority of cases, ASF was detected in small holdings and backyards. The biosecurity measures are not officially required by veterinary regulation and are only given in a form of recommendations. On the other hand, it is not always possible to implement biosecurity measures that are recognized today as essential for sustainable pig production in the old type of industrial pig facilities. Nowadays, in 2021, it became obvious that the domestic pig cycle, human activities involving pigs, or pig-derived meat products are the dominant driver of virus transmission. Additionally, human activities are frequently a risky connection between domestic pigs and wild boars both directly or indirectly. Traditional, culture-related aspects and facts that politicians failed to recognise ASF as a serious issue that causes great economical losses were found to be very important obstacles in disease control.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"71 1","pages":"371 - 391"},"PeriodicalIF":0.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43779815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: a retrospective study of 2432 Cases (2011 – Mid 2021) 塞尔维亚犬肿瘤性和非肿瘤性皮肤病变的患病率和分布:2432例病例的回顾性研究(2011-2021年年中)
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2021-12-01 DOI: 10.2478/acve-2021-0034
V. Kukolj, S. Nešić, D. Marinković, S. Aleksić-Kovačević
{"title":"Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: a retrospective study of 2432 Cases (2011 – Mid 2021)","authors":"V. Kukolj, S. Nešić, D. Marinković, S. Aleksić-Kovačević","doi":"10.2478/acve-2021-0034","DOIUrl":"https://doi.org/10.2478/acve-2021-0034","url":null,"abstract":"Abstract Cutaneous lesions, especially skin tumors in dogs, are among the most common lesions in this animal species. The aim of this study was to identify the most common types of canine cutaneous lesions, to determine the absolute and relative frequency of each type of cutaneous lesion, anatomical locations, mean age, as well as gender and breed distribution. The examination included all samples of cutaneous lesions in dogs obtained by surgical biopsy in veterinary clinics and examined at the Laboratory of the Department of Pathology at the Faculty of Veterinary Medicine, University of Belgrade from the 1st January 2011 to the 1st July 2021. In this period (126 months), a total of 2432 samples of cutaneous lesions were examined, of which 1984 (81.58%) were tumors (1037/1984, 52.27% benign and 947/1984, 47.73% malignant) and 448 (18.42%) non-neoplastic cutaneous lesions. The most commonly found cutaneous tumors were: mast cell tumor (17.34% of all tumors), histiocytoma (9.78%), papilloma (7.91%), lipoma (7.81%), squamous cell carcinoma (7.36%), trichoblastoma (4.44%), hepatoid adenoma (4.39%) and malignant melanoma (4.18%). The most common non-neoplastic cutaneous lesions were: follicular cyst(s) (35.04% of all non-neoplastic lesions), pyogranulomatous chronic dermatitis (23.88%), lymphocytic dermatitis (7.37%), hyperkeratosis (4.24%), and granulomatous dermatitis (3.79%). Our results substantially confirm previously reported data regarding cutaneous neoplastic and nonneoplastic lesions in dogs, and provide updated information on their frequency, animal age, anatomic location and breed distributions.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"71 1","pages":"403 - 416"},"PeriodicalIF":0.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49387021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive 不同诊断方法对病理性产褥期奶牛亚临床子宫内膜炎的鉴别及对妊娠的可靠性评价
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2021-12-01 DOI: 10.2478/acve-2021-0038
Bojana Bajagić, J. Mrkun, D. Kirovski, Đ. Savić, D. Budimir, J. Maletić, Milan Maletic
{"title":"Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive","authors":"Bojana Bajagić, J. Mrkun, D. Kirovski, Đ. Savić, D. Budimir, J. Maletić, Milan Maletic","doi":"10.2478/acve-2021-0038","DOIUrl":"https://doi.org/10.2478/acve-2021-0038","url":null,"abstract":"Abstract The aim of study was to evaluate different methods for subclinical endometritis (SCE) diagnoses and their accuracy to predict pregnancy. The study was performed on 51 postpartum cross breed dairy cows with pathological puerperium. Artificial insemination (AI) was done in spontaneous estrus. Endometrium samples were taken by cytotape (CT), cervical mucus (CM) samples by Metricheck and smears for crystallization degree were obtained at AI day. Blood serum samples for progesterone (P4) concentration determination were taken at AI day and 17 days later. Cows were examined by ultrasound 35 days after AI to confirm pregnancy. Percent (%) of polymorphnuclear leukocytes (PMNs) taken by CT were expressed by ROC curve (with cut-off point set at 1%). Out of the total of 51 cows involved in the study, 13.73% true positive and 41.17% true negative cows were found (54.90%). The content of CM sampled with Metricheck was altered in 11 cows (21.57%), and one of them was pregnant (1.96%). An average macroscopic CM value for pregnant cows was 0.06±0.24 vs. 0.39±0.79 for non-pregnant (p<0.05). An average value of CM crystallization in pregnant cows was 2.50±1.04 vs. 1.85±1.15 in non-pregnant (p<0.05). P4 concentration was not statistically different at AI day, but was statistically significantly higher 17 days later in pregnant cows (p = 0.002). CT alterations may be a reliable diagnostic tool for SCE diagnosis, while macroscopic CM alterations may be used for CE detection. P4 determination is beneficial in pregnancy diagnosis while CM crystallization degree is helpful in determination of best time for AI.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"71 1","pages":"462 - 476"},"PeriodicalIF":0.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43148581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Characterisation of AmpC / ESBL genes in some pathogen gram-negatives isolated from clinical cases of livestock and companion animals 从家畜和伴侣动物的临床病例中分离的一些病原体革兰氏阴性中AmpC/ESBL基因的特征
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2021-12-01 DOI: 10.2478/acve-2021-0036
F. Pehlivanoğlu, E. Sababoglu
{"title":"Characterisation of AmpC / ESBL genes in some pathogen gram-negatives isolated from clinical cases of livestock and companion animals","authors":"F. Pehlivanoğlu, E. Sababoglu","doi":"10.2478/acve-2021-0036","DOIUrl":"https://doi.org/10.2478/acve-2021-0036","url":null,"abstract":"Abstract This study was aimed to search and characterize the AmpC and/or ESBL genes of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from clinical cases of local livestock and companion animals between 2017 and 2019. A total of eight ceftiofur-resistant E. coli (n= 7) and ceftiofur-resistant K. pneumoniae (n= 1) and seven P. aeruginosa were isolated from different cases in local animals. By combination disc method, six E. coli isolates and one K. pneumoniae isolate were found to be ESBL producers. By combination of the disc method and double disc synergy test, no P. aeruginosa isolates were found as ESBL producers. In the agar disc diffusion test (ADDT) performed with cefoxitin and cefoxitin-boronic, only one E. coli was determined as AmpC producer. In ESBL-producing isolates, only the CTX-M class gene was detected by polymerase chain reaction (PCR) and subsequent sequence analysis revealed CTX-M-3 and CTX-M-15 variants. An AmpC positive E. coli isolate was found to carry plasmidic ampC gene in cmy-2 variant from CIT family. It was observed that P. aeruginosa isolates did not carry the plasmidic ampC gene. After the chromosomal ampC gene of one P. aeruginosa was amplified by PCR and sequenced, R79Q and T105A mutations in the chromosomal ampC gene was revealed. This showed that overproduction of the ampC enzyme is involved in the resistance to β-lactams in P. aeruginosa isolates in the study.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"71 1","pages":"435 - 450"},"PeriodicalIF":0.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49394194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genotypic correlation between Salmonella Enteritidis isolates from broiler breeders and hatchery flocks 肉鸡种鸡和孵化场肠炎沙门氏菌分离株的基因型相关性研究
IF 0.6 4区 农林科学
Acta Veterinaria-Beograd Pub Date : 2021-12-01 DOI: 10.2478/acve-2021-0033
İ. B. Müştak, H. K. Müştak, Seyyide Sarıçam İnce
{"title":"Genotypic correlation between Salmonella Enteritidis isolates from broiler breeders and hatchery flocks","authors":"İ. B. Müştak, H. K. Müştak, Seyyide Sarıçam İnce","doi":"10.2478/acve-2021-0033","DOIUrl":"https://doi.org/10.2478/acve-2021-0033","url":null,"abstract":"Abstract In this study, Salmonella Enteritidis strains isolated from dust and environmental materials from different flocks located in Turkey’s Western Black Sea region were examined by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). A total of 59 S. Enteritidis strains isolated from broiler breeder and hatchery flocks, and one S. Enteritidis strain isolated from a stool sample of a farm worker were examined. PFGE analysis revealed two major PFGE groups and nine different macro restriction profiles. It was determined that 85% (51/60) of the strains were close to each other and comprised Group I. All S. Enteritidis strains had the same sequence type (ST): ST11. Isolation of strains with a single genotype suggests that there may be a cross transmission between the flocks.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"71 1","pages":"392 - 402"},"PeriodicalIF":0.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45999459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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