高效液相色谱-紫外分光光度法测定马血浆中替地匹罗辛含量的建立与验证

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria-Beograd Pub Date : 2022-03-01 DOI:10.2478/acve-2022-0008

J. S. Galecio, V. Hernandis, E. Escudero, P. Marín

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引用次数: 3

摘要

摘要建立了一种简便、快速、低成本、灵敏的高效液相色谱法测定马血浆中替地吡嗪的方法。血浆样品用乙醚提取，蒸发后，用波长为289nm的紫外检测器通过反相色谱法测定替地吡嗪。Tildipirosin在Zorbax Eclipse XDB-C18柱上分离，150 x 3.0 mm，5μm，梯度色谱洗脱。替地吡嗪和酒石酸泰乐菌素的保留时间分别为3.0分钟和6.4分钟。该方法的总运行时间为9分钟。校准曲线范围为0.1至3μg/mL。血浆检测下限为0.035μg/mL，定量下限为0.1μg/mL。准确度和精密度始终<12%，LLOQ<20%除外。平均回收率为99.5%。该程序可用于测定血浆中替地吡嗪的浓度，并可用于进行药代动力学研究。

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Development and Validation of an Improved HPLC-UV Method for the Determination of Tildipirosin in Horse Plasma

Abstract A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in horse plasma. Plasma samples were extracted with diethyl ether, and after evaporation, tildipirosin was determined by reverse-phase chromatography with an ultraviolet detector set at a wavelength of 289 nm. Tildipirosin was separated on a Zorbax Eclipse XDB-C18 column, 150 x 3.0 mm, 5 μm with gradient chromatographic elution. The retention times were 3.0 min and 6.4 min for tildipirosin and tylosin tartrate, respectively. The total run time was 9 minutes in this method. Calibration curves ranged from 0.1 to 3 μg/mL. The lower limit of detection for plasma was0.035μg/mL, and the lower limit of quantitation was 0.1 μg/mL. Both accuracy and precision were always < 12% exce pt for LLOQ < 20%. Mean recovery was 99.5 %. This procedure can be applied to determine tildipirosin concentrations in plasma and be useful to perform pharmacokinetic studies.

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来源期刊

Acta Veterinaria-Beograd 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

18-36 weeks

期刊介绍： The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.