J. Grahovac, Milenka Ivanković, R. Dekić, S. Paraš
{"title":"Effects of Diazepam on Hematological and Histological Parameters in Rats / in Vivo and Unbiased Stereological Investigation","authors":"J. Grahovac, Milenka Ivanković, R. Dekić, S. Paraš","doi":"10.2478/acve-2022-0019","DOIUrl":"https://doi.org/10.2478/acve-2022-0019","url":null,"abstract":"Abstract Diazepam-based drugs are widely used today in human treatment. Diazepam may be a primary drug aimed at treating neurological diseases or an associated drug in the treatment of other diseases in the purpose of symptomatic therapy. The sedative effect of diazepam characterizes it as a drug that people usually use on their own and without a doctor’s supervision. Directly, but also through influencing the nervous system, diazepam disrupts proper functioning of all body organs. The purpose of this paper was to examine the effects of diazepam on blood and cytohistological parameters of rats in an in vivo experiment. Mallory-Azan and immunochistochemical staining methods BLX-CX and Survivin tissues of liver, kidney and spleen of rats were used to achieve the set goal. Cytometric analysis of rats detected cells in apoptosis and measurements of stereological parameters were made using a system according to Cavalier’s principle. Results of analysis of hematological and histological parameters indicate a detrimental effect of diazepam on blood parameters, as well as on structure and functioning of the liver, kidneys and spleen of rats. This paper is a foundation for further detailed scientific research with the aim of elucidating all harmful effects that diazepam has on all organs in the body of rats. This data could serve as a starting point for future studies in clinical pharmacology on therapeutic protocols for usage of diazepam-based sedatives.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"235 - 255"},"PeriodicalIF":0.6,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48617356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Gavrilović, V. Stojiljković, S. Pejić, Vera Spasojević Tišma, D. Nikolic, S. Pajović
{"title":"Differences in the Functional Activity and Redox Homeostasis Between the Left and Right Adrenal Gland of Rats Exposed to Chronic Isolation Stress","authors":"L. Gavrilović, V. Stojiljković, S. Pejić, Vera Spasojević Tišma, D. Nikolic, S. Pajović","doi":"10.2478/acve-2022-0018","DOIUrl":"https://doi.org/10.2478/acve-2022-0018","url":null,"abstract":"Abstract The aim of this study was to examine whether there are differences in adrenomedullary function in respect to the left and right sides in chronic stress conditions. We investigated how chronic stress isolation (CSI 12 weeks) affected the protein levels of key enzymes involved in adrenaline (A) synthesis (phenyl ethanolamine N-methyltransferase -PNMT), storage (vesicular monoamine transporters 2 - VMAT2) and degradation (catechol-O-methyltransferase - COMT), as well as the concentrations of A as an index for adrenomedullary function in the left and right adrenal medulla. Also, we examined the concentrations of malondialdehyde (MDA), protein levels of nuclear factor κB (NF-κB), and activity of catalase (CAT) in the left and right adrenal medulla. The investigated parameters were quantified by Western blot analysis, assay of enzymatic activity, and CAT Research ELISA kits. We found that CSI pro duced significantly increased levels of PNMT protein, and VMAT2 protein, as well as increased concentrations of A in the right adrenal medulla. However, we recorded that CSI increased protein levels of COMT and NF-κB, as well as the concentrations of MDA in the left adrenal medulla. Also, CSI decreased the activity of CAT only in the left adrenal medulla. Based on these results, it may be concluded that adrenomedullary function is different in respect to the left and right sides in chronic stress conditions.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"224 - 234"},"PeriodicalIF":0.6,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42454291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Adamiak, P. Jastrzębski, J. Snarska, Lucjan Samson
{"title":"Orthosis in the Conservative Treatment of Cranial Cruciate Ligament Rupture in Dogs – Clinical Observations","authors":"Z. Adamiak, P. Jastrzębski, J. Snarska, Lucjan Samson","doi":"10.2478/acve-2022-0009","DOIUrl":"https://doi.org/10.2478/acve-2022-0009","url":null,"abstract":"Abstract Orthoses are increasingly frequently applied orthopedic devices in veterinary medicine. Injury to the knee joint with a rupture of the cranial cruciate ligament is one of the main indications for the use of orthoses in dogs. This study aimed to present the results of treatment of 30 dogs with injured cranial cruciate ligaments using a knee orthosis and describe the reasons for the conservative treatment of cranial cruciate ligament deficiency. The study was conducted between 2017 and 2019 and involved 30 dogs of various breeds aged 7 to 15, weighing from 2.5 kg to 45 kg, diagnosed with cranial cruciate ligament rupture. In the study, a stifle knee orthosis by the Polish manufacturer Admirał (Pl) was used in 29 dogs and, in one case, an orthosis by Balto (USA) was applied. In each case, the orthosis was selected individually, based on the measurements taken. In the twelfth week of treatment using orthoses, the owners of 25 dogs were satisfied with the results of conservative treatment with the application of orthoses. The caretakers of four dogs observed difficulties in the form of slight lameness at rest after prolonged exertion on the previous day. Running difficulty was observed in one dog. In the conducted study, the knee orthosis demonstrated good therapeutic effects in dogs up to 25 kg. In the majority of dogs, a slight increase in the flexion angle of the treated knee joints was observed, X-ray examinations did not show the degenerative disease progress during the studied period.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"111 - 117"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43020449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Tomanić, B. Božin, I. Čabarkapa, N. Kladar, M. Radinović, Milan Maletic, Z. Kovačević
{"title":"Chemical Composition, Antioxidant and Antibacterial Activity of Two Different Essential Oils Against Mastitis Associated Pathogens","authors":"D. Tomanić, B. Božin, I. Čabarkapa, N. Kladar, M. Radinović, Milan Maletic, Z. Kovačević","doi":"10.2478/acve-2022-0004","DOIUrl":"https://doi.org/10.2478/acve-2022-0004","url":null,"abstract":"Abstract Mastitis is one of the most common and costly diseases affecting dairy cows worldwide. Since antibiotic resistance has become a global threat to both animal and human health, it is becoming more urgent to continuously search for new therapeutical alternatives for the control and treatment of bovine mastitis. Hence, our research aimed to test the therapeutic use of two essential oils (EOs) based on their chemical composition, antibacterial and antioxidant potential. The present study was conducted by collecting milk samples from the cows diagnosed with clinical or subclinical mastitis with the aim of isolating and identifying bacterial strains. The antioxidant potential of essential oils of Menthae piperitae (MP) and Melissa officinalis (MO) was evaluated in several in vitro assays. In the MP EO, a total of 38 compounds were identified, with menthol as the dominant compound, whereas in MO EO 51 compounds were identified. Furthermore, the values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) have been used to quantitatively measure the antibacterial activity of each essential oil. In accordance with which, MP EO samples exhibited a higher degree of antibacterial activity than MO EO. Thus, EOs have been shown to be promising alternatives to antibiotics because of their availability, biodegradability, and lower risk of side effects as compared with conventional, antimicrobial treatment. Nevertheless, further clinical studies are needed to test the potential role of EOs in treating mastitis in dairy cows.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"45 - 58"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69088603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Bogunović, Nina Dominiković, Nemanja M. Jovanović, K. Nenadović, Z. Kulišić, T. Ilić, N. Stevic
{"title":"Environmental Contamination by Parasites in Public Parks in Belgrade in the Context of One Health Approach","authors":"D. Bogunović, Nina Dominiković, Nemanja M. Jovanović, K. Nenadović, Z. Kulišić, T. Ilić, N. Stevic","doi":"10.2478/acve-2022-0003","DOIUrl":"https://doi.org/10.2478/acve-2022-0003","url":null,"abstract":"Abstract This paper studies the presence of developmental forms of gastrointestinal parasites in soil and sand samples, as well as in dog feces collected from public green spaces in Belgrade. The paper incorporates the spread analysis of contamination over different segments of parks. Four public green spaces in Belgrade were chosen, all containing an open-spaced children’s playground and a fenced dog park. Sample analysis of soil/sand was examined using qualitative methods without concentration (native slide) and with concentration (passive sedimentation and gravitational centrifugal flotation). In total, 106 samples have been collected out of which 60 samples of soil, 36 of dog faeces and ten samples of sand. Seven different agents have been detected, out of which five nematodes - Ancylostoma/Uncinaria spp., Trichuris spp., Capillaria spp., Toxocara spp., Toxascaris leonina and two protozoa - Isospora spp. and Giardia intestinalis. The overall prevalence of contamination of soil samples was 31.67% and a statistically significant difference in the prevalence of ancylostoma eggs was found between different locations. The most common agent detected in the dog’s faeces was Isospora spp., with a prevalence of 5.56%, followed by Giardia intestinalis and ancylostomatids with a prevalence of 2.78% each. The sand samples had no parasitic elements found. The contamination by parasites and by dogs’ faeces was equally dispersed in all segments of the examined locations. There is a great need to raise public awareness on the issue, and by the joint action of veterinarians, medical doctors, pet owners and people using public parks for recreation - a precondition is created for the sustainability of the “One Health” concept which implies the preservation of the environment and human and animal health.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"30 - 44"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43785375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Branislav Pešić, Z. Kulišić, R. Teodorović, S. Trailović, V. Djokic, M. Djordjevic
{"title":"Comparison of Mosquito Larvicidal Formulations of Diflubenzuron on Culex Pipiens Mosquitoes in Belgrade, Serbia","authors":"Branislav Pešić, Z. Kulišić, R. Teodorović, S. Trailović, V. Djokic, M. Djordjevic","doi":"10.2478/acve-2022-0007","DOIUrl":"https://doi.org/10.2478/acve-2022-0007","url":null,"abstract":"Abstract Culex mosquitos are important infectious diseases vectors in temperate and tropical regions of the World, affecting nearly 350 million people in both developed and developing countries. Our approach was to “recycle” the well-established larvicide, and by studying the tablets, pellets and granules as floating or sinking formulations, we found a method to optimise the use of diflubenzuron against Culex pipiens mosquitoes in field conditions. A standard WHO procedure was used to test the larvicide efficacy. The combined efficacy of all floating formulations was 10.7% higher than sinking preparations (p-value =0.002) and that maximised throughout the experiment on days 14 and 21, (p-values 0.012 and 0.008, respectively). All floating formulations kept their efficacies above 70% until day 21 of the experiment, while the mortality of sinking designs dropped significantly after day 14. The lowest efficacy was observed when sinking granules were used and the highest when floating tablets were applied in the canals. Only the floating tablets showed no significant changes in efficacy from day 1 to 21, but then that efficacy drops sharp until day 35. Since the larvae spend most of their time on the surface of the slow-moving waters to breathe, and floating pellets and tablets are made of materials that can serve as food sources, we conclude that these formulations have a higher efficacy, at least in conditions of non- or slow-moving waters. This study shows the importance of a systematic approach to reformulation of old, already proven and environmentally safe larvicides which can control the mosquito populations and their spreading of various pathogens.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"87 - 99"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45997475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Case of Leporine Dysautonomia from Croatia","authors":"D. Huber, A. G. Kurilj, I. Šoštarić-Zuckermann","doi":"10.2478/acve-2022-0010","DOIUrl":"https://doi.org/10.2478/acve-2022-0010","url":null,"abstract":"Abstract Leporine dysautonomia (or dysautonomia of hares) is an idiopathic disorder associated with degeneration of neurons of the peripheral nervous system with loss of sympathetic and parasympathetic nerve function. The disease has so far been described in rabbits and hares from Great Britain, and a similar disorder has also been described in horses (grass sickness), dogs and cats (Key-Gaskell syndrome) throughout the world. We describe a case of leporine dysautonomia in a rabbit from Croatia, characterized by gross findings of cachexia, dehydration, dilated intestines and urinary bladder. Histologic findings included severe vacuolation of neurons of the submucous and myenteric plexus in the intestinal tract. This disease presents a rare disorder of rabbits, and based on current report, it should be considered as a possible differential diagnosis is rabbits with neurologic clinical signs and mild gross findings including cachexia and distension of intestine and urinary bladder.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"118 - 123"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44243224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jovana Čukuranović Kokoris, V. Ajdžanović, L. Pendovski, N. Ristić, V. Milošević, Monika Dovenska, F. Popovska-Perčinić
{"title":"The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study","authors":"Jovana Čukuranović Kokoris, V. Ajdžanović, L. Pendovski, N. Ristić, V. Milošević, Monika Dovenska, F. Popovska-Perčinić","doi":"10.2478/acve-2022-0001","DOIUrl":"https://doi.org/10.2478/acve-2022-0001","url":null,"abstract":"Abstract Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"1 - 15"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42870412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. G. Borges, Mário Henrique Conde, Cibele Cristina Tavares Cunha, M. G. de Freitas, Elio Moro, Fernando de Almeida Borges
{"title":"Moxidectin: A Viable Alternative for the Control of Ivermectin-Resistant Gastrointestinal Nematodes in Beef Cattle","authors":"D. G. Borges, Mário Henrique Conde, Cibele Cristina Tavares Cunha, M. G. de Freitas, Elio Moro, Fernando de Almeida Borges","doi":"10.2478/acve-2022-0002","DOIUrl":"https://doi.org/10.2478/acve-2022-0002","url":null,"abstract":"Abstract The increasing prevalence of anthelmintic resistance in cattle especially for avermectins, is a challenge for controlling parasites in some herds. Thus, field studies demonstrating the increase in productivity by the use of anthelmintic formulations, even when a suboptimal treatment (efficacy below 95%), can contribute to the development of gastrointestinal nematodes control programs in beef cattle. The objective of the present study was to evaluate the anthelmintic efficacy and productive performance in pasture-raised beef calves, treated with macrocyclic lactones. A Split plot in time randomized block design was used to assess weight gain and reduction in fecal egg count (FECs) of treatments: 1% moxidectin (1% MOX), ivermectin (IVM) and abamectin (ABM) (2.25% IVM+1.25% ABM), 4% IVM, 3.15% IVM and placebo. For the evaluation of FECs and weight gain of the animals, individual samples were collected seven days before treatment and, +14, +30, +56, +91 and +118 days post-treatment (DPT). The efficacies in the 14th DPT were: 72.3% (1% MOX), 22.1% (4% IVM), 22% (2.25% IVM + 1.25% ABM) and 0% (3.15% ivermectin). 1% MOX was the only treatment that resulted in a significant increase in weight gain of the animals compared to the placebo group after 118 days of treatment, with a difference of 7.6 kg. Therefore, MOX remains a viable alternative for the control of helminths resistant to avermectins and still capable of resulting in significant productive gains, even with an efficacy below 95%.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"16 - 29"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45663516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Mihajlović, M. Cincović, D. Nakov, B. Stanković, J. Miočinović, S. Hristov
{"title":"Improvement of Hygiene Practices and Milk Hygiene Due to Systematic Implementation of Preventive and Corrective Measures","authors":"L. Mihajlović, M. Cincović, D. Nakov, B. Stanković, J. Miočinović, S. Hristov","doi":"10.2478/acve-2022-0006","DOIUrl":"https://doi.org/10.2478/acve-2022-0006","url":null,"abstract":"Abstract The Total Bacterial Count (TBC) and the Somatic Cells Count (SCC) in the milk are important indicators of its hygiene and quality. Hygienic conditions in barns, milking procedures, udder hygiene before, during and after milking, milking machine hygiene, as well as milk cooling procedures immediately after milking, have direct or indirect influences on milk hygiene indicators. Poor results of milk hygiene quality, when it comes to SCC and TBC, which are often seen in dairy cattle farms in our country, indicate numerous omissions before and during milking. To determine the possibility of improving milk hygiene quality, training of extension service professionals and farmers was conducted, corrective and preventive measures were determined and the achieved state was monitored on 128 dairy farms where SCC and TBC were observed at regular monthly intervals for 6 months. The results showed a continuous statistically very significant improvement in hygienic conditions in barns, milking procedures, udder hygiene before, during and after milking, milking machine hygiene, as well as milk cooling procedures. This has contributed to a statistically very significant improvement in the hygienic quality of milk, both in SCC and TBC indicators, continuously from month to month, with a visible improvement at the end related to the beginning of the study period. At the begining and the end of survey 19.7% and 50.0% of milk samples belong to 1st class of milk quality, respectively, indicating a significant improvement after implementation of corrective measurements.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":"72 1","pages":"76 - 86"},"PeriodicalIF":0.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44549075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}