The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria-Beograd Pub Date : 2022-03-01 DOI:10.2478/acve-2022-0001

Jovana Čukuranović Kokoris, V. Ajdžanović, L. Pendovski, N. Ristić, V. Milošević, Monika Dovenska, F. Popovska-Perčinić

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Abstract

Abstract Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.

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中高温长期暴露对大鼠垂体ACTH细胞影响的组织学和激素研究

摘要全球变暖导致夏季环境温度升高和热浪延长，这是影响哺乳动物下丘脑-垂体-肾上腺皮质（HPA）轴的应激因素。本研究的目的是研究长期（7-60天）暴露于中等升高的环境温度（35±1°C）对成年大鼠垂体促肾上腺皮质（ACTH）细胞的组织学特征和分泌能力以及皮质酮输出的影响。ACTH细胞的立体结构参数通过免疫组化进行估计。通过免疫测定法测定血液中ACTH和皮质酮的浓度。与对照组相同参数相比，中高温暴露7、14、21、30和60天的大鼠ACTH细胞体积分别减少了18.1%、14.5%、13.5%、8.6%和14.2%（p<0.05）。高温暴露7、14、21、30和60天组ACTH细胞体积密度分别比对照组降低40.0%、33.3%、26.7%、13.3%和26.7%（p<0.05）。ACTH的血浆浓度随着暴露于高温的持续时间而不同（p<0.05）。中高温组血清皮质酮浓度分别比对照组降低54.9%、24.4%、29.9%、21.1%和24.4%（p<0.05）。尽管在治疗过程中ACTH细胞有一些功能恢复的迹象，但人们的印象是，这种应激源的长期特性克服了HPA轴的抵抗能力。

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来源期刊

Acta Veterinaria-Beograd 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

18-36 weeks

期刊介绍： The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.