Chemical Composition, Antioxidant and Antibacterial Activity of Two Different Essential Oils Against Mastitis Associated Pathogens

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria-Beograd Pub Date : 2022-03-01 DOI:10.2478/acve-2022-0004

D. Tomanić, B. Božin, I. Čabarkapa, N. Kladar, M. Radinović, Milan Maletic, Z. Kovačević

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引用次数: 7

Abstract

Abstract Mastitis is one of the most common and costly diseases affecting dairy cows worldwide. Since antibiotic resistance has become a global threat to both animal and human health, it is becoming more urgent to continuously search for new therapeutical alternatives for the control and treatment of bovine mastitis. Hence, our research aimed to test the therapeutic use of two essential oils (EOs) based on their chemical composition, antibacterial and antioxidant potential. The present study was conducted by collecting milk samples from the cows diagnosed with clinical or subclinical mastitis with the aim of isolating and identifying bacterial strains. The antioxidant potential of essential oils of Menthae piperitae (MP) and Melissa officinalis (MO) was evaluated in several in vitro assays. In the MP EO, a total of 38 compounds were identified, with menthol as the dominant compound, whereas in MO EO 51 compounds were identified. Furthermore, the values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) have been used to quantitatively measure the antibacterial activity of each essential oil. In accordance with which, MP EO samples exhibited a higher degree of antibacterial activity than MO EO. Thus, EOs have been shown to be promising alternatives to antibiotics because of their availability, biodegradability, and lower risk of side effects as compared with conventional, antimicrobial treatment. Nevertheless, further clinical studies are needed to test the potential role of EOs in treating mastitis in dairy cows.

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两种不同精油对乳腺炎相关病原体的化学成分、抗氧化和抗菌活性

乳腺炎是影响奶牛的最常见和最昂贵的疾病之一。由于抗生素耐药性已成为对动物和人类健康的全球性威胁，因此不断寻找控制和治疗牛乳腺炎的新疗法变得更加紧迫。因此，我们的研究旨在测试两种精油(EOs)基于其化学成分，抗菌和抗氧化潜力的治疗用途。本研究通过收集诊断为临床或亚临床乳腺炎的奶牛的牛奶样本进行，目的是分离和鉴定细菌菌株。通过体外实验对薄荷精油(Menthae piperitae, MP)和茉莉精油(Melissa officinalis, MO)的抗氧化能力进行了评价。在MP - EO中共鉴定出38个化合物，其中薄荷醇为优势化合物，而在MO - EO中鉴定出51个化合物。此外，最低抑菌浓度(mic)和最低杀菌浓度(MBCs)的值被用来定量测量每种精油的抗菌活性。由此可见，MP - EO样品的抑菌活性高于MO - EO样品。因此，与传统的抗菌治疗相比，EOs具有可获得性、可生物降解性和更低的副作用风险，因此已被证明是抗生素的有希望的替代品。然而，需要进一步的临床研究来测试EOs在治疗奶牛乳腺炎中的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Veterinaria-Beograd 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

18-36 weeks

期刊介绍： The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.