不同诊断方法对病理性产褥期奶牛亚临床子宫内膜炎的鉴别及对妊娠的可靠性评价

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES
Bojana Bajagić, J. Mrkun, D. Kirovski, Đ. Savić, D. Budimir, J. Maletić, Milan Maletic
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引用次数: 2

摘要

摘要本研究的目的是评估亚临床子宫内膜炎(SCE)诊断的不同方法及其预测妊娠的准确性。对51头产后病理性产褥期杂交奶牛进行了研究。人工授精是在自然发情期进行的。子宫内膜标本采用CT,宫颈粘液(CM)标本采用Metricheck,并于AI日进行结晶度涂片检查。在AI日和17天后采集用于测定孕酮(P4)浓度的血清样品。奶牛在人工流产后35天接受超声波检查以确认怀孕。CT采集的多形核白细胞(PMN)的百分比(%)用ROC曲线表示(截止点设置为1%)。在参与研究的51头奶牛中,13.73%的真阳性奶牛和41.17%的真阴性奶牛(54.90%)。Metricheck采样的11头奶牛(21.57%)的CM含量发生了变化,妊娠奶牛的平均宏观CM值为0.06±0.24,非妊娠奶牛为0.39±0.79(p<0.05),妊娠奶牛的CM结晶平均值为2.50±1.04,非妊娠母牛为1.85±1.15(p<0.05),但在怀孕17天后有统计学意义(p=0.002)。CT改变可能是SCE诊断的可靠诊断工具,而宏观CM改变可用于CE检测。P4测定有助于妊娠诊断,CM结晶度有助于AI最佳时机的确定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive
Abstract The aim of study was to evaluate different methods for subclinical endometritis (SCE) diagnoses and their accuracy to predict pregnancy. The study was performed on 51 postpartum cross breed dairy cows with pathological puerperium. Artificial insemination (AI) was done in spontaneous estrus. Endometrium samples were taken by cytotape (CT), cervical mucus (CM) samples by Metricheck and smears for crystallization degree were obtained at AI day. Blood serum samples for progesterone (P4) concentration determination were taken at AI day and 17 days later. Cows were examined by ultrasound 35 days after AI to confirm pregnancy. Percent (%) of polymorphnuclear leukocytes (PMNs) taken by CT were expressed by ROC curve (with cut-off point set at 1%). Out of the total of 51 cows involved in the study, 13.73% true positive and 41.17% true negative cows were found (54.90%). The content of CM sampled with Metricheck was altered in 11 cows (21.57%), and one of them was pregnant (1.96%). An average macroscopic CM value for pregnant cows was 0.06±0.24 vs. 0.39±0.79 for non-pregnant (p<0.05). An average value of CM crystallization in pregnant cows was 2.50±1.04 vs. 1.85±1.15 in non-pregnant (p<0.05). P4 concentration was not statistically different at AI day, but was statistically significantly higher 17 days later in pregnant cows (p = 0.002). CT alterations may be a reliable diagnostic tool for SCE diagnosis, while macroscopic CM alterations may be used for CE detection. P4 determination is beneficial in pregnancy diagnosis while CM crystallization degree is helpful in determination of best time for AI.
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来源期刊
Acta Veterinaria-Beograd
Acta Veterinaria-Beograd 农林科学-兽医学
CiteScore
1.30
自引率
16.70%
发文量
33
审稿时长
18-36 weeks
期刊介绍: The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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