Investigation of the Effects of L-carnitine and magnesium on Oxidative Stress and Cytokines in the Tissue of Experimental diabetic rats

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria-Beograd Pub Date : 2021-12-01 DOI:10.2478/acve-2021-0039

Meryem Senturk, M. Eren, Z. Sarıca

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引用次数: 0

Abstract

Abstract The aim of this study was to determine the effects of L-carnitine and magnesium on the levels of tissue malondialdehyde, 8-hydroxy-2’-deoxyguanosine, and cytokines (tumor necrosis factor alpha, interleukin-6) in streptozotocin-induced experimental diabetes in rats. Eighty male Wistar albino rats (200-250 g) were divided into 8 groups with 10 rats in each group. The groups received the following treatments: Control group; 2 ml distilled water (by gavage); Group 2: 50 mg/kg (b.w.) i.p. streptozotocin; Group 3: 125 mg/kg (b.w.) magnesium; Group 4: 300 mg/kg (b.w.) L-carnitine; Group 5: 125 mg/kg (b.w.) magnesium +300 mg/kg (b.w.) L-carnitine; Group 6: 50 mg/kg (b.w.) streptozotocin +125 mg/kg (b.w.) magnesium; Group 7: 50 mg/kg (b.w.) streptozotocin +300 mg/kg (b.w.) L-carnitine and Group 8: 50 mg/kg (b.w.) streptozotocin +125 mg/ kg (b.w.) magnesium+300 mg/kg (b.w.) L-carnitine administered for 4 weeks. Liver and kidney malondialdehyde, 8-hydroxy-2’-deoxyguanosine, tumor necrosis factor alpha and interleukin-6 levels did not change in the magnesium, L-carnitine, and magnesium + L-carnitine groups compared to the control. The highest levels of malondialdehyde, 8-hydroxy-2’-deoxyguanosine, tumor necrosis factor alpha and interleukin-6 were determined only in the group with diabetes (Group 2). Lipid peroxidation, DNA damage, and cytokine levels were significantly reduced in diabetic animals with the administration of magnesium and L-carnitine separately or in combination. Based on the obtained results it can be concluded that magnesium and L-carnitine may have antidiabetic effects, especially in combination.

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L-肉碱和镁对实验性糖尿病大鼠组织氧化应激和细胞因子影响的研究

摘要本研究的目的是确定L-肉碱和镁对链脲佐菌素诱导的实验性糖尿病大鼠组织丙二醛、8-羟基-2'-脱氧鸟苷和细胞因子（肿瘤坏死因子α、白细胞介素-6）水平的影响。将80只雄性Wistar白化大鼠（200-250g）分为8组，每组10只。两组分别接受以下治疗：对照组；2 ml蒸馏水（灌胃）；第2组：50 mg/kg（b.w.）腹腔注射链脲佐菌素；第3组：125 mg/kg（体重）镁；第4组：300 mg/kg（b.w.）L-肉碱；第5组：125 mg/kg（b.w.）镁+300 mg/kg（b.w.）左旋肉碱；第6组：50 mg/kg链脲佐菌素+125 mg/kg镁；第7组：50 mg/kg（b.w.）链脲佐菌素+300 mg/kg（b.w..）L-肉碱，第8组：50 g/kg（b.w..）链脲佐菌素+125 mg/kg（b.w.）镁+300 mg/kg L-肉碱，给药4周。与对照组相比，镁、L-肉碱和镁+L-肉碱组的肝脏和肾脏丙二醛、8-羟基-2'-脱氧鸟苷、肿瘤坏死因子α和白细胞介素-6水平没有变化。丙二醛、8-羟基-2'-脱氧鸟苷、肿瘤坏死因子-α和白细胞介素-6的最高水平仅在糖尿病组中测定（第2组）。单独或联合施用镁和左旋肉碱可显著降低糖尿病动物的脂质过氧化、DNA损伤和细胞因子水平。根据所获得的结果，可以得出结论，镁和L-肉碱可能具有抗糖尿病作用，尤其是联合使用。

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来源期刊

Acta Veterinaria-Beograd 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

18-36 weeks

期刊介绍： The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.