Biocell最新文献_第6页

CPT1A in cancer: Tumorigenic roles and therapeutic implications CPT1A在癌症中的致瘤作用和治疗意义

4区生物学

Biocell Pub Date : 2023-01-01 DOI: 10.32604/biocell.2023.027677

SHENGJIE SONG, ZHIZHOU SHI

引用次数: 0

Possible therapeutic role of short-chain fatty acids from skin commensal bacteria in UVB-induced skin carcinogenesis 来自皮肤共生菌的短链脂肪酸在uvb诱导的皮肤癌发生中的可能治疗作用

4区生物学

Biocell Pub Date : 2023-01-01 DOI: 10.32604/biocell.2023.030383

PAVITHRA SUBRAMANI, RAUNAK KUMAR DAS

{"title":"Possible therapeutic role of short-chain fatty acids from skin commensal bacteria in UVB-induced skin carcinogenesis","authors":"PAVITHRA SUBRAMANI, RAUNAK KUMAR DAS","doi":"10.32604/biocell.2023.030383","DOIUrl":"https://doi.org/10.32604/biocell.2023.030383","url":null,"abstract":"Solar ultraviolet B (UVB) radiation is a major skin cancer-causing agent. Initiation, promotion, and progression are the diverse phases of UVB-induced carcinogenesis. Exposure to UVB causes abnormalities in a series of biochemical and molecular pathways: thymine dimer formation, DNA damage, oxidative stress, inflammatory responses, and altered cell signaling, eventually resulting in tumor formation. The increased skin cancer rates urge researchers to develop more efficient drugs, but synthetic chemotherapeutic drugs have more contrary effects and drug resistance issues, which have been reported recently. The current review focuses on the relationship between microbes and cancer. Human skin acts as a barrier against the external environment and serves as a protective shield for its inhabitant microbiota, collectively called skin microbes. The gut microbiome plays a vital role in cancer therapy. Production of short-chain fatty acids (SCFAs) such as butyrate, acetate, and propionate by intestinal microbes has anti-cancer properties against various cancer cell lines. Yet, the knowledge of SCFAs produced by skin microbes remains yet to be elucidated exhaustively. In this review, we strive to summarize the findings of studies performed to date regarding the anti-cancer properties of SCFA against various cancer cell lines and provide insight into future directions in the skin microbiome field.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135504584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HOXB8 contributed to oxaliplatin chemo-resistance in colon cancer cells by activating STAT3 HOXB8通过激活STAT3参与结肠癌细胞对奥沙利铂的耐药

4区生物学

Biocell Pub Date : 2023-01-01 DOI: 10.32604/biocell.2023.030147

LIANLI NI, YUN YU, HAN LIN, WEISHAN ZHUGE, LU TAO, YIWEI SHEN, RI CUI, SHAOTANG LI

{"title":"HOXB8 contributed to oxaliplatin chemo-resistance in colon cancer cells by activating STAT3","authors":"LIANLI NI, YUN YU, HAN LIN, WEISHAN ZHUGE, LU TAO, YIWEI SHEN, RI CUI, SHAOTANG LI","doi":"10.32604/biocell.2023.030147","DOIUrl":"https://doi.org/10.32604/biocell.2023.030147","url":null,"abstract":"Background: Homeobox B8 (HOXB8), a member of HOX family, plays a key role in the development of colorectal cancer (CRC). However, the function of HOXB8 in oxaliplatin (OXA) resistance in CRC is still unclear. This study investigated the role and precise molecular mechanism of HOXB8 in OXA-resistant CRC cells. Methods: The cell viability was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, and the colony forming ability was determined by colony formation assay. The silencing RNA (siRNA) approach was used to knockdown HOXB8 in CRC cells while the lentiviral transfection system was used to establish stable HOXB8 overexpressing CRC cells. The protein and mRNA levels were evaluated by western blot and real-time reverse transcription-polymerase chain reaction. Results: HOXB8 expression was upregulated in OXA-resistant HCT116 cells (HCT116/OXA) compared to its level in the parent HCT116 cells. Knockdown of HOXB8 significantly inhibited CRC cell growth by suppressing the signal transducer and activator of transcription 3 (STAT3) pathway. HOXB8 knockdown also potentiated cytotoxicity of OXA in CRC cells. Inversely, HOXB8 overexpression attenuated OXA-induced growth inhibition of HCT116 cells and RKO cells by activating STAT3 signaling. HOXB8 knockdown effectively inhibited HCT116/OXA cell viability regardless of OXA treatment by suppressing STAT3 signaling. Conclusions: These results shed light on the important functions of HOXB8 in OXA-resistant CRC and suggested that targeting HOXB8 might be an effective therapeutic strategy for select OXA-resistant CRC patients.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135505229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long non-coding RNA-ATB induces trastuzumab resistance and aggravates the progression of gastric cancer by repressing miR- 200c via ZNF217 elevation 长链非编码RNA-ATB通过ZNF217升高抑制miR- 200c，诱导曲妥珠单抗耐药，并加重胃癌进展

4区生物学

Biocell Pub Date : 2023-01-01 DOI: 10.32604/biocell.2023.029860

JIAZHUANG LI, WEI ZHANG, SHOUBAO GAO, LI SUN, QINGYANG TAI, YING LIU

引用次数: 0

Inhibition of VEGF-A expression in hypoxia-exposed fetal retinal microvascular endothelial cells by exosomes derived from human umbilical cord mesenchymal stem cells 来自人脐带间充质干细胞的外泌体抑制缺氧暴露的胎儿视网膜微血管内皮细胞中VEGF-A的表达

4区生物学

Biocell Pub Date : 2023-01-01 DOI: 10.32604/biocell.2023.044177

JING LI, WANWAN FAN, LILI HAO, YONGSHENG LI, GUOCHENG YU, WEI SUN, XIANQIONG LUO, JINGXIANG ZHONG

{"title":"Inhibition of VEGF-A expression in hypoxia-exposed fetal retinal microvascular endothelial cells by exosomes derived from human umbilical cord mesenchymal stem cells","authors":"JING LI, WANWAN FAN, LILI HAO, YONGSHENG LI, GUOCHENG YU, WEI SUN, XIANQIONG LUO, JINGXIANG ZHONG","doi":"10.32604/biocell.2023.044177","DOIUrl":"https://doi.org/10.32604/biocell.2023.044177","url":null,"abstract":": Objective: This study aimed to investigate the potential of human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes (hucMSC-Exos) in inhibiting hypoxia-induced cell hyper proliferation and overexpression of vascular endothelial growth factor A (VEGF-A) in immature human fetal retinal microvascular endothelial cells (hfRMECs). Methods: Exosomes were isolated from hucMSCs using cryogenic ultracentrifugation and characterized through various techniques, including transmission electron microscopy, nanoparticle tracking analysis, bicinchoninic acid assays, and western blotting. The hfRMECs were identi ﬁ ed using von Willebrand factor (vWF) co-staining and divided into four groups: a control group cultured under normoxic condition, a hypoxic model group, a hypoxic group treated with low-concentration hucMSC-Exos (75 μ g/mL) and a hypoxic group treated with high-concentration hucMSC-Exos (100 μ g/mL). Cell viability and proliferation were assessed using Cell Counting Kit-8 (CCK-8) assay and EdU (5-ethynyl-2 ′ -deoxyuridine) assay respectively. Expression levels of VEGF-A were evaluated using RT-PCR, western blotting and immuno ﬂ uorescence. Results: Hypoxia signi ﬁ cantly increased hfRMECs ’ viability and proliferation by upregulating VEGF-A levels. The administration of hucMSC-Exos effectively reversed this response, with the high-concentration group exhibiting greater ef ﬁ cacy compared to the low-concentration group. Conclusion: In conclusion, hucMSC-Exos can dose-dependently inhibit hypoxia-induced hyperproliferation and VEGF-A overexpression in immature fetal retinal microvascular endothelial cells.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135563776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive analysis of the role of molecular docking in the development of anticancer agents against the cell cycle CDK enzyme 分子对接在抗细胞周期CDK酶抗癌药物开发中的作用综合分析

IF 1.2 4区生物学

Biocell Pub Date : 2023-01-01 DOI: 10.32604/biocell.2023.026615

P. Solanki, Nisarg Rana, Prakash C. JHA, A. Manhas

引用次数: 2

TianmaGouteng yin attenuates ischemic stroke-induced brain injury by inhibiting the AGE/RAGE pathway 天麻骨藤饮通过抑制AGE/RAGE通路减轻缺血性脑损伤

IF 1.2 4区生物学

Biocell Pub Date : 2023-01-01 DOI: 10.32604/biocell.2023.028866

Luojun Zheng, Luan Weng, Diwen Shou

引用次数: 0

Single-cell sequencing analysis reveals the molecular mechanism of promotion of SCAP proliferation upon AZD2858 treatment 单细胞测序分析揭示了AZD2858促进SCAP增殖的分子机制