4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2023.045884

Daolun Yu, DEYONG SHE, Kai Ge, Lei Yang, RUINA ZHAN, Shan Lü, Yafei Cai

{"title":"Smad8 is involvement in follicular development via the regulation of granulosa cell growth and steroidogenesis in mice","authors":"Daolun Yu, DEYONG SHE, Kai Ge, Lei Yang, RUINA ZHAN, Shan Lü, Yafei Cai","doi":"10.32604/biocell.2023.045884","DOIUrl":"https://doi.org/10.32604/biocell.2023.045884","url":null,"abstract":"Background: SMAD family proteins (SMADs) are crucial transcription factors downstream of transforming growth factor beta (TGF-ß)/SMAD signaling pathways that have been reported to play a pivotal role in mammalian reproduction. However, the role of SMAD family member 8 (SMAD8, also known as SMAD9), a member of the SMAD family, in mammalian reproduction remains unclear. Methods: We employed RNA interference techniques to knock down Smad8 expression in mouse granulosa cells (GCs) to investigate the effects of Smad8 on GC growth and steroidogenesis. Results: Our findings revealed a significant decrease in the proliferative capacity and a substantial increase in the apoptosis rate of GCs after transfection with Smad8-siRNA for 48 h. Subsequent hormone assays demonstrated a significant decrease in estradiol (E2) levels, whereas progesterone (P4) remained unchanged. Further mechanistic analysis showed that the mRNA expression of proliferating cell nuclear antigen (Pcna), Cyclin D2, cell cycle-dependent kinase 4 (Cdk4), B-cell lymphoma-2 (Bcl-2), estrogen receptor (Er), luteinizing hormone receptor (Lhr) and cytochrome P450 family 19 subfamily A member 1 (Cyp19a1) significantly decreased. Conversely, the mRNA of cysteine aspartate proteinase 3 (Caspase 3) significantly increased, wheras Bcl2-associated X (Bax), follicle-stimulating hormone receptor (Fshr) and cytochrome P450 family 11 subfamily A member 1 (Cyp11a1) remained unchanged compared to the controls. Conclusion: This study indicates that Smad8 knockdown inhibits cell proliferation, promotes apoptosis, reduces Er and Lhr transcription, and decreases E2 production in mouse GCs. These findings suggest that Smad8 may serve as a novel genetic marker for mammalian reproduction.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"48 1","pages":"139-147"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.techscience.com/biocell/online/detail/19708/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell transcriptomics reveals T-cell heterogeneity and immunomodulatory role of CD4+ T native cells in Candida albicans infection 单细胞转录组学揭示了CD4&lt； sup<+</sup&gt； T原生细胞在白色念珠菌感染中的T细胞异质性和免疫调节作用

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.051383

Ke-Ran Jia

{"title":"Single-cell transcriptomics reveals T-cell heterogeneity and immunomodulatory role of CD4+ T native cells in Candida albicans infection","authors":"Ke-Ran Jia","doi":"10.32604/biocell.2024.051383","DOIUrl":"https://doi.org/10.32604/biocell.2024.051383","url":null,"abstract":"Objective: Candida albicans is a common fungal pathogen that triggers complex host defense mechanisms, including coordinated innate and adaptive immune responses, to neutralize invading fungi effectively. Exploring the immune microenvironment has the potential to inform the development of therapeutic strategies for fungal infections. Methods: The study analyzed individual immune cell profiles in peripheral blood mononuclear cells from Candida albicans-infected mice and healthy control mice using single-cell transcriptomics, fluorescence quantitative PCR, and Western blotting. We investigated intergroup differences in the dynamics of immune cell subpopulation infiltration, pathway enrichment, and differentiation during Candida albicans infection. Results: Our findings indicate that infiltration of CD4 naive cells, regulatory T (Treg) cells, and Microtubules-associated (MT)-associated cells increased after infection, along with impaired T cell activity. Notably, CD4 T cells and plasma cells were enhanced after infection, suggesting that antibody production is dependent on T cells. In addition, we screened 6 hub genes, transcription factor forkhead box protein 3(Foxp3), cytotoxic T-lymphocyte associated protein 4 (CTLA4), Interleukin 2 Receptor Subunit Beta (Il2rb), Cd28, C-C Motif Chemokine Ligand 5 (Ccl5), and Cd27 for alterations associated with CD4 T cell differentiation. Conclusions: These results provide a comprehensive immunological landscape of the mechanisms of Candida albicans infection and greatly advance our understanding of adaptive immunity in fungal infections.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"48 9","pages":"1355-1368"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interferon-gamma regulates the progression of neuroblastoma cells through interferon-regulatory factor-1 干扰素- γ通过干扰素调节因子-1调节神经母细胞瘤细胞的进展

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.051673

Jing Wang, JUNFENG XU, YINGRAN YANG, YOUZHENG QIU, Shanshan Zhang, Ning Wang

{"title":"Interferon-gamma regulates the progression of neuroblastoma cells through interferon-regulatory factor-1","authors":"Jing Wang, JUNFENG XU, YINGRAN YANG, YOUZHENG QIU, Shanshan Zhang, Ning Wang","doi":"10.32604/biocell.2024.051673","DOIUrl":"https://doi.org/10.32604/biocell.2024.051673","url":null,"abstract":"Objective: This study aimed to elucidate the influence of IFN-gamma (IFN-γ) in neuroblastoma (NB) cells and reveal its potential underlying molecular mechanism. Methods: The Cell Counting Kit-8, Transwell apparatus, and flow cytometry were employed to assess cellular viability, migratory capacity, invasive potential, and apoptotic rates, respectively. RNA-seq combined with bioinformatics analysis revealed differentially expressed genes (DEGs) and their possible biological functions. Protein levels were determined by western blot analysis. Results: IFN-γ treatment resulted in diminished cell viability, mitigated migratory and invasive capabilities, and augmented apoptotic activity in the SK-N-BE (2) cell line, whereas it exhibited the opposite effect in SH-SY5Y cells. Furthermore, interferon regulatory factor 1 (IRF-1) was the common DEG in both IFN-γ-treated SK-N-BE (2) and SH-SY5Y cells. Additionally, we found that it was underexpressed in NB tissues. The depletion of IRF-1 promoted the progression of both SK-N-BE (2) and SH-SY5Y cells. Moreover, IRF-1 knockdown effectively counteracted the effects of IFN-γ on SK-N-BE (2) cells, while exacerbating them in SH-SY5Y cells. Conclusion: This study verified that IFN-γ exerted a distinct role in both N-Myc-and non-N-Myc-amplified NB cells, partially by mediating the expression of IRF-1, suggesting that it may serve as a potent agent for treating patients with NB.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"48 9","pages":"1343-1353"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.techscience.com/biocell/online/detail/21257/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in bioenergetics and neuroprotective properties of mesenchymal stromal cells after LPS treatment LPS处理后间充质间质细胞的生物能量学和神经保护特性的变化

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.058496

Elmira I. Yakupova, Alexey D. Bocharnikov, Kseniya S. Fedulova, Valentina A. Babenko, Д. Н. Силачев, Egor Y. Plotnikov

引用次数: 0

Glycyrrhizic acid alleviates lung injury in sepsis through SIRT1/HMGB1 pathway 甘草酸通过SIRT1/HMGB1通路减轻脓毒症肺损伤

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.053652

BINGJIE LIN, XIAOBO YING, C N Zhang, Guojun Zhang

引用次数: 5

miR-557 suppresses hepatocellular carcinoma cell proliferation and migration via downregulating CBX4 miR-557通过下调CBX4抑制肝癌细胞的增殖和迁移

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.050519

Xulong Sun, WENTAO DING, Chao Jiang, Zhian Fang

{"title":"miR-557 suppresses hepatocellular carcinoma cell proliferation and migration via downregulating CBX4","authors":"Xulong Sun, WENTAO DING, Chao Jiang, Zhian Fang","doi":"10.32604/biocell.2024.050519","DOIUrl":"https://doi.org/10.32604/biocell.2024.050519","url":null,"abstract":"Introduction: Hepatocellular carcinoma (HCC), a prevalent malignancy, poses significant challenges with high tumor heterogeneity and poor prognosis. MicroRNAs (miRNAs) play a pivotal role in hepatocarcinogenesis. Although abnormalities in microRNA-557 (miR-557) expression have been implicated in various cancer types, its role in HCC remains unclear. Therefore, there is a need to explore the function of microRNA-557 in HCC. Methods: Candidate miRNAs were identified through screening in GSE108724 and GSE20077. Real-time PCR was employed to analyze the expression level of miR-557 in hepatoma cell lines and tissues. Cell viability and migration assays were applied to assess the impact of miR-557 on HCC cell lines. Furthermore, the miR-557 target was predicted through three algorithms (Targetscan, miRWalk, and miRanda), and this was confirmed through luciferase assay and Western blotting. Results: In this study, miR-557 was identified in two datasets and expressed at a low level in both hepatoma cell lines and tissues. Notably, high expression of miR-557 in HCC cells inhibited oncogenesis. Conversely, low expression of miR-557 enhanced tumor proliferation and migration. Polycomb chromobox 4 (CBX4) was identified as a direct target of miR-557. Silencing CBX4 influenced the functional impact of miR-557 on HCC cell migration. Conclusion: Taken together, our study contributed to elucidating the hepatoma molecular heterogeneity and provided novel insights into miR-557 role and its target CBX4 in HCC, suggesting its potential as a future effectively druggable target for HCC intervention.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"48 7","pages":"1071-1079"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.techscience.com/biocell/online/detail/20475/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Meiotic nuclear divisions 1 suppresses the proliferation and invasion of pancreatic cancer cells via regulating H2A.X variant histone 减数分裂核分裂1通过调节H2A抑制胰腺癌细胞的增殖和侵袭。X变异组蛋白

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2023.046903

DONGQIN WANG, Yan Shi, ZHIQIANG WANG, Jing Zhang, Luyao Wang, Hongyu Ma, SHUHUA SHI, XIAOFU LIAN, HUA HUANG, XIAOJING WANG, CHAOQUN LIAN

{"title":"Meiotic nuclear divisions 1 suppresses the proliferation and invasion of pancreatic cancer cells via regulating H2A.X variant histone","authors":"DONGQIN WANG, Yan Shi, ZHIQIANG WANG, Jing Zhang, Luyao Wang, Hongyu Ma, SHUHUA SHI, XIAOFU LIAN, HUA HUANG, XIAOJING WANG, CHAOQUN LIAN","doi":"10.32604/biocell.2023.046903","DOIUrl":"https://doi.org/10.32604/biocell.2023.046903","url":null,"abstract":"Introduction: Among all malignant tumors of the digestive system, pancreatic carcinoma exhibits the highest mortality rate. Currently, prevention and effective treatment are urgent issues that need to be addressed. Methods: The study focused on meiotic nuclear divisions 1 (MND1), integrating data from the Gene Expression Profiling Interactive Analysis (GEPIA) database with prognostic survival analysis. Simultaneously, experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC. The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA (siRNA) in Patu-8988 and Panc1 cell lines. Results: The results of Cell Counting Kit-8 indicated that the suppression of MND1 resulted in a decrease in cell proliferation. Wound healing and Transwell assays revealed that MND1 knockdown reduced cell migration and invasion. Flow cytometry revealed that inhibiting MND1 hindered the cell cycle. Furthermore, MND1 could stimulate the proliferation, migration, and invasion of Patu-8988 and Panc1 cells by increasing the expression of MND1. Notably, MND1 had a positive effect on H2AFX expression in PC cells. Elevated MND1 expression suggests the low overall survival rate of individuals diagnosed with PC. Conclusion: These findings suggest that MND1 has the potential to be a gene with the ability to accurately diagnose and treat PC.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"48 1","pages":"111-122"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.techscience.com/biocell/online/detail/19684/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147914245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Tanshinone IIA inhibits NLRP3 activation and attenuates alveolar macrophage pyroptosis via the TREM2/β-catenin pathway 丹参酮IIA通过TREM2/β-catenin途径抑制NLRP3活化，减轻肺泡巨噬细胞焦亡

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.053227

Min Liu, Xia Li, Jun Liu, Yu Liu

引用次数: 2

ABHD17C represses apoptosis and pyroptosis in hepatocellular carcinoma cells ABHD17C抑制肝癌细胞凋亡和焦亡

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.051756

LINPEI WANG, JIAWEI WANG, Chun‐Feng Shi, Wei Wang, Jian Wu

引用次数: 4

Biological function of miRNA-145-5p in angiotensin II induced renal inflammation miRNA-145-5p在血管紧张素II诱导的肾脏炎症中的生物学功能

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.047404

Bin Li, YUCHENG SHENG, Xiaoying Xu, SHENGCUN WANG, HONGYAN SONG, JINGYUAN LI, HAONAN JI, QINGHUA WANG, Xiaodi Zhou, Longju Qi

{"title":"Biological function of miRNA-145-5p in angiotensin II induced renal inflammation","authors":"Bin Li, YUCHENG SHENG, Xiaoying Xu, SHENGCUN WANG, HONGYAN SONG, JINGYUAN LI, HAONAN JI, QINGHUA WANG, Xiaodi Zhou, Longju Qi","doi":"10.32604/biocell.2024.047404","DOIUrl":"https://doi.org/10.32604/biocell.2024.047404","url":null,"abstract":"Objective:: Chronic kidney disease (CKD) is a progressive disorder characterized by intricate structural and functional alterations in the kidneys, attributable to diverse causative factors. Notably, the therapeutic promise of miR-145-5p in addressing renal pathologies has been discerned. This investigation seeks to elucidate the functional role of miR-145-5p in injured kidneys by subjecting human glomerular mesangial cells (HGMCs) to stimulation with Angiotensin II (AngII). Materials and Methods:: Cellular viability and the levels of inflammatory mediators were evaluated utilizing Cell Counting Kit-8 (CCK-8), quantitative real-time polymerase chain reaction (qRT-PCR), and western blot methodologies, both in the presence of AngII incubation and in scenarios of miR-145p overexpression and downregulation. Furthermore, the cell cycle dynamics were elucidated through Fluorescence-activated Cell Sorting (FACS) analysis. Results:: AngII incubation induced an upregulation of miR-145-5p and inflammatory factors including Intercellular Adhesion Molecule 1 (ICAM-1), Interleukin 6 (IL-6), Interleukin 8 (IL-8), and Interleukin 1β (IL-1β). Additionally, it elevated the expression of Cyclin A2, Cyclin D1, and the G2/M cell cycle ratio. Conversely, inhibition of miR-145-5p heightened the levels of inflammatory factors and cell cycle regulators induced by AngII incubation. Reduced expression of miR-145-5p correlated with a downregulation of Interleukin 10 (IL-10) expression, concurrently promoting HGMC proliferation under AngII stimulation. Moreover, ectopic miR-145-5p expression demonstrated a reduction in inflammatory factors, cell cyclin regulators, G2/M cell cycle ratio, and overall proliferation. Conclusion:: MiR-145-5p exhibited inhibitory effects on the inflammatory response and proliferation induced by Angiotensin II in HGMCs, showcasing its potential as a therapeutic avenue for the treatment of kidney injury.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"48 4","pages":"601-611"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.techscience.com/biocell/online/detail/20239/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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