4区生物学

Biocell Pub Date : 2023-01-01 DOI: 10.32604/biocell.2023.044177

JING LI, WANWAN FAN, LILI HAO, YONGSHENG LI, GUOCHENG YU, WEI SUN, XIANQIONG LUO, JINGXIANG ZHONG

{"title":"Inhibition of VEGF-A expression in hypoxia-exposed fetal retinal microvascular endothelial cells by exosomes derived from human umbilical cord mesenchymal stem cells","authors":"JING LI, WANWAN FAN, LILI HAO, YONGSHENG LI, GUOCHENG YU, WEI SUN, XIANQIONG LUO, JINGXIANG ZHONG","doi":"10.32604/biocell.2023.044177","DOIUrl":"https://doi.org/10.32604/biocell.2023.044177","url":null,"abstract":": Objective: This study aimed to investigate the potential of human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes (hucMSC-Exos) in inhibiting hypoxia-induced cell hyper proliferation and overexpression of vascular endothelial growth factor A (VEGF-A) in immature human fetal retinal microvascular endothelial cells (hfRMECs). Methods: Exosomes were isolated from hucMSCs using cryogenic ultracentrifugation and characterized through various techniques, including transmission electron microscopy, nanoparticle tracking analysis, bicinchoninic acid assays, and western blotting. The hfRMECs were identi ﬁ ed using von Willebrand factor (vWF) co-staining and divided into four groups: a control group cultured under normoxic condition, a hypoxic model group, a hypoxic group treated with low-concentration hucMSC-Exos (75 μ g/mL) and a hypoxic group treated with high-concentration hucMSC-Exos (100 μ g/mL). Cell viability and proliferation were assessed using Cell Counting Kit-8 (CCK-8) assay and EdU (5-ethynyl-2 ′ -deoxyuridine) assay respectively. Expression levels of VEGF-A were evaluated using RT-PCR, western blotting and immuno ﬂ uorescence. Results: Hypoxia signi ﬁ cantly increased hfRMECs ’ viability and proliferation by upregulating VEGF-A levels. The administration of hucMSC-Exos effectively reversed this response, with the high-concentration group exhibiting greater ef ﬁ cacy compared to the low-concentration group. Conclusion: In conclusion, hucMSC-Exos can dose-dependently inhibit hypoxia-induced hyperproliferation and VEGF-A overexpression in immature fetal retinal microvascular endothelial cells.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135563776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}