Biocell最新文献

{"title":"Reversal of maternal obesity attenuates hypoxia and improves placental development in the preeclamptic-like BPH/5 mouse model.","authors":"Daniella M Adams, Kalie F Beckers, Juliet P Flanagan, Viviane C L Gomes, Chin-Chi Liu, Jenny L Sones","doi":"10.32604/biocell.2023.029644","DOIUrl":"10.32604/biocell.2023.029644","url":null,"abstract":"<p><strong>Background: </strong>Women with obesity have higher risk of adverse pregnancy outcomes, including preeclampsia (PE). Late-gestational hypertension, aberrant fetoplacental development, and fetal growth restriction (FGR), hallmarks of PE, are observed spontaneously in BPH/5 mice. Similar to obese preeclamptic women, BPH/5 mice have higher visceral white adipose tissue (WAT) and circulating leptin. We hypothesized that attenuation of maternal obesity and serum leptin in pregnant BPH/5 mice will improve fetoplacental development by decreasing hypoxia markers and leptin expression at the maternal-fetal interface.</p><p><strong>Methods: </strong>To test this hypothesis, BPH/5 mice were fed <i>ad libitum</i> (lib) and pair-fed (PF) to C57 ad lib controls beginning at embryonic day (e) 0.5. Hypoxia-related genes, hypoxia inducible factor (Hif) 1α, stem cell factor (Scf), heme oxygenase-1 (Ho-1), leptin (Lep), and leptin receptor (LepR) were assessed in e7.5 implantation sites.</p><p><strong>Results: </strong>BPH/5 ad lib had 1.5 to 2-fold increase in <i>Hif1α</i>, <i>Scf</i>, and <i>Ho-1</i> mRNA and a greater than 3-fold increase in leptin mRNA <i>vs</i>. C57 that was attenuated with PF. Exogenous leptin promoted Hif1α and Ho-1 mRNA expression in e7.5 decidua <i>in vitro</i>. While hypoxic conditions <i>in vitro</i> did not change decidual leptin mRNA. Furthermore, BPH/5 PF mice demonstrated improved fetal and placental outcomes later in gestation, with greater placental vascular area by e18.5 and attenuation of FGR.</p><p><strong>Conclusion: </strong>In conclusion, pair-feeding BPH/5 mice beginning at conception may improve placental vasculature formation via decreased leptin and hypoxia-associated markers in this model. Future investigations are needed to better determine the effect of hypoxia and leptin on pregnancy outcomes in obese pregnant women.</p>","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41220811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-proliferative effect of <i>Annona</i> extracts on breast cancer cells.","authors":"Maria-Luisa Veisaga, Mariam Ahumada, Stacy Soriano, Leonardo Acuna, Wei Zhang, Ivy Leung, Robert Barnum, Manuel A Barbieri","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Backgorund: </strong>Fruits and seed extracts of Annona montana have significant cytotoxic potential in several cancer cells. This study evaluates the effect of A. montana leaves hexane extract on several signaling cascades and gene expression in metastatic breast cancer cells upon insulin-like growth factor-1 (IGF-1) stimulation.</p><p><strong>Methods: </strong>MTT assay was performed to determine the proliferation of cancer cells. Propidium iodide staining and flow cytometry analysis of Annexin V binding was utilized to measure the progression of the cell cycle and the induction of apoptosis. Protein expression and phosphorylation were determined by western blotting analysis to examine the underlying cellular mechanism triggered upon treatment with A. <i>montana</i> leaves hexane extract.</p><p><strong>Results: </strong>A. <i>montana</i> leaves hexane (sub-fraction V) blocked the constitutive stimulation of the PI3K/mTOR signaling pathways. This inhibitory effect was associated with apoptosis induction as evidenced by the positivity with Annexin V and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNNEL) staining, activation of caspase-3, and cleavage of PPAR. It also limited the expression of various downstream genes that regulate proliferation, survival, metastasis, and angiogenesis (i.e., cyclin D1, survivin, COX-2, and VEGF). It increased the expression of p53 and p21. Interestingly, we also observed that this extract blocked the activation of AKT and ERK without affecting the phosphorylation of the IGF-1 receptor and activation of Ras upon IGF-1 stimulation.</p><p><strong>Conclusion: </strong>Our study indicates that A. <i>montana</i> leaves (sub-fraction V) extract exhibits a selective anti-proliferative and proapoptotic effect on the metastatic MDA-MB-231 breast cancer cells through the involvement of PI3K/AKT/mTOR/S6K1 pathways.</p>","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538365/pdf/nihms-1927735.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41152106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of non-enzymatic glycation on collagen nanoscale mechanisms in diabetic and age-related bone fragility.","authors":"James L Rosenberg,&nbsp;William Woolley,&nbsp;Ihsan Elnunu,&nbsp;Julia Kamml,&nbsp;David S Kammer,&nbsp;Claire Acevedo","doi":"10.32604/biocell.2023.028014","DOIUrl":"https://doi.org/10.32604/biocell.2023.028014","url":null,"abstract":"<p><p>Age and diabetes have long been known to induce an oxidative reaction between glucose and collagen, leading to the accumulation of advanced glycation end-products (AGEs) cross-links in collagenous tissues. More recently, AGEs content has been related to loss of bone quality, independent of bone mass, and increased fracture risk with aging and diabetes. Loss of bone quality is mostly attributed to changes in material properties, structural organization, or cellular remodeling. Though all these factors play a role in bone fragility disease, some common recurring patterns can be found between diabetic and age-related bone fragility. The main pattern we will discuss in this viewpoint is the increase of fibrillar collagen stiffness and loss of collagen-induced plasticity with AGE accumulation. This study focused on recent related experimental studies and discusses the correlation between fluorescent AGEs content at the molecular and fibrillar scales, collagen deformation mechanisms at the nanoscale, and resistance to bone fracture at the macroscale.</p>","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10293963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal miR-30a-5p targets NLRP3 to suppress podocyte pyroptosis in diabetic nephropathy","authors":"Wei Lu, Kan Guo, Dianmei Xi, Zhaoxia Xia","doi":"10.32604/biocell.2023.024591","DOIUrl":"https://doi.org/10.32604/biocell.2023.024591","url":null,"abstract":": Background: Mesenchymal stem cell (MSC)-derived exosomes are closely related to pyroptosis in diabetic nephropathy (DN). This study aimed to explore the protective effect of exosomal miR-30a-5p on podocyte pyroptosis in DN. Methods: Streptozotocin was used to establish the mouse model of DN. Human bone marrow MSC-derived exosomes were extracted and identi fi ed via transmission electron microscopy, nanoparticle tracking analysis, and western blotting. MiR-30a-5p mimics and non-control (NC) mimics were transfected into MSCs and podocytes, and exosomes were isolated from the MSCs. High glucose (HG)-induced podocyte model was established to determine the effect of exosomal miR-30a-5p on pyroptosis and in fl ammation in vitro . Results: MiR-30a-5p was expressed at low levels in DN models, while NLR family pyrin domain containing 3 (NLRP3), caspase-1, gasdermin-N (GSDMD-N), and pro-in fl ammatory factors (tumor necrosis factor-alpha, interleukin (IL)-1beta, and IL-18) were augmented. In vitro , miR-30a-5p expression in the HG-damaged podocytes was down-regulated, while NLRP3 was up-regulated. Interestingly, miR-30a-5p overexpression diminished HG-induced podocyte injury, as proven by increased activity and decreased pyroptosis of podocytes. Concurrently, the up-regulation of miR-30a-5p could inhibit the expression of pro-in fl ammatory factors, caspase-1, GSDMD-N, and NLRP3 in HG-induced podocytes. MSC-derived exosomal miR-30a-5p treatment of HG-damaged cells has similar effects to miR-30a-5p mimics treatment. Overexpression of NLRP3 reversed the effect of miR-30a-5p mimics on HG-induced podocytes. Conclusion: This research con fi rmed that exosomal miR-30a-5p regulates pyroptosis via mediating NLRP3 in DN.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73838290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A developed ant colony algorithm for cancer molecular subtype classification to reveal the predictive biomarker in the renal cell carcinoma","authors":"Zekun Xin, Yudan Ma, Weiqiang Song, Hao Gao, Lijun Dong, Bao Zhang, Zhilong Ren","doi":"10.32604/biocell.2023.026254","DOIUrl":"https://doi.org/10.32604/biocell.2023.026254","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73886396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic analysis of lncRNA-associated competing endogenous RNA regulatory networks in synovial tissue of temporomandibular joint osteoarthritis","authors":"Chuyao Wang, Chuan Lu, L. Zou, D. He","doi":"10.32604/biocell.2023.028199","DOIUrl":"https://doi.org/10.32604/biocell.2023.028199","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78205175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional factor RUNX1: A potential therapeutic target for fibrotic pulmonary disease","authors":"Jia Liu, Faping Wang, Bo Yuan, F. Luo","doi":"10.32604/biocell.2023.026148","DOIUrl":"https://doi.org/10.32604/biocell.2023.026148","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78381608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of H2O2-induced apoptosis of GC2-spg cells by functionalized selenium nanoparticles with lentinan through ROS-mediated ERK/p53 signaling pathways","authors":"Miaomiao Li, Danyang Chen, Junyi Ke, Ruilin Zheng, Jingyao Su, Z. Zheng, Jieyi Luo, Hanran Mai, Fan Jiang, Yan-xia Qu, Xiaoqiong Gu, B. Zhu, Yinghua Li, Liandong Zuo","doi":"10.32604/biocell.2023.025154","DOIUrl":"https://doi.org/10.32604/biocell.2023.025154","url":null,"abstract":"A H2O2-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of seleniumcontaining antioxidant enzymes induced by oxidative stress. A safe and effective nano-drug system of functionalized selenium-containing nanoparticles (SeNPs) was developed with lentinan (LNT) (SeNPs@LNT). Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT (1, 2, 4, 8, 16, 32 μM) for 24–72 h to evaluate the cytotoxicity of selenium. GC2-spg cells were randomly divided into the following groups: control, hydrogen peroxide (H2O2), SeNPs@LNT, and H2O2+SeNPs@LNT groups. H2O2+SeNPs@LNT group was pretreated with SeNPs@LNT 4 μM for 12 h, followed by H2O2 600 μM for 8 h. The cell viability decreased in the H2O2 group and increased significantly in the SeNPs@LNT group. Compared with the H2O2 group, the SeNPs@LNT+H2O2 group exhibited obvious red fluorescence, indicating a higher level of mitochondrial membrane potential. The content of intracellular reactive oxygen species (ROS) in the SeNPs@LNT group reduced significantly, and the intensity of green fluorescence in the SeNPs@LNT+H2O2 group decreased significantly compared with the H2O2 group, indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress. The activity of GPx and SOD increased significantly in the SeNPs@LNT group. The expression of p53 decreased significantly under the intervention of nano-selenium, and GPx1 expression increased. In the oxidative stress group, the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups. Thus, SeNPs@LNT can promote GC2-spg cell proliferation, improve GPx and SOD activities, remove intracellular ROS, and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels. SeNPs@LNT has good biological activity and antioxidant effect, which can be used to protect the male reproductive system from","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77390487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression profiles of circulating tRNA-derived small RNAs and their potential role in diabetes","authors":"Jing Jin, Xie Li, Ting Qiu, Lei Song, Yuanyue Cui, Guangya Zhang, Shu Li, Wencheng Zhao","doi":"10.32604/biocell.2023.029493","DOIUrl":"https://doi.org/10.32604/biocell.2023.029493","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80257166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The extracellular secretion of miR-1825 wrapped by exosomes increases CLEC5A expression: A potential oncogenic mechanism in ovarian cancer","authors":"Qiaoling Wu, Zhaolei Cui, Hongmei Xia, Shan Jiang, Jing Bai, Z. Shao, Yang Sun","doi":"10.32604/biocell.2023.027494","DOIUrl":"https://doi.org/10.32604/biocell.2023.027494","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84512268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}