抗氧化剂trolox抑制衰老，促进间充质干细胞分泌前列腺素E-2

Pub Date : 2023-01-01 DOI:10.32604/biocell.2023.025203

Xiaoxu Zhang, Lin Zhang, Lin Du, Huiyan Sun, Xia Zhao, Yang Sun, Wen Wang, Lisheng Wang

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引用次数: 0

摘要

间充质干细胞(Mesenchymal stem cells, MSCs)因其具有增殖、分化和免疫调节等功能而广泛应用于再生医学和临床治疗。然而，在体外培养过程中，MSCs容易老化，这在很大程度上限制了其应用。前列腺素E-2 (Prostaglandin E-2, PGE-2)是间充质干细胞分泌的发挥免疫调节作用的关键效应因子。通过筛选PGE-2分泌的化合物文库，证实了抗氧化剂trolox是MSCs分泌PGE-2的刺激物。研究了抗氧化剂trolox对MSCS老化、增殖和基因表达等生物学特性的影响。结果表明，trolox在不影响MSCs表面标记物表达的情况下，具有抗衰老、促进增殖、促进PGE-2分泌的作用。此外，trolox处理上调MSCs中的miR-17-92簇，并可能有助于其抗衰老作用。因此，加入trolox可能有利于MSCs的扩增和应用。

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The antioxidant trolox inhibits aging and enhances prostaglandin E-2 secretion in mesenchymal stem cells

: Mesenchymal stem cells (MSCs) have been widely used in regenerative medicine and clinical therapy due to their capabilities of proliferation, differentiation, and immune regulation. However, during in vitro expansion, MSCs are prone to aging, which largely limits their application. Prostaglandin E-2 (PGE-2) is a key effector secreted by MSCs to exert immunomodulatory effects. By screening the compound library for PGE-2 secretion, the antioxidant trolox was veri ﬁ ed as a stimulator of MSCs to secrete PGE-2. The effect of antioxidant trolox on biological characteristics of MSCS, including aging, proliferation, and gene expression, was examined. The results demonstrated that trolox can resist aging, promote proliferation, and enhance PGE-2 secretion of MSCs without affecting their surface marker expression. Furthermore, trolox treatment up-regulates miR-17-92 clusters in MSCs and may contribute to its anti-aging effects. Thus, trolox addition might be bene ﬁ cial for MSCs expansion and their application.

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