番红花提取物对乳腺癌症细胞的抗增殖作用。

IF 0.8 4区 生物学 Q4 BIOLOGY
Biocell Pub Date : 2023-08-28
Maria-Luisa Veisaga, Mariam Ahumada, Stacy Soriano, Leonardo Acuna, Wei Zhang, Ivy Leung, Robert Barnum, Manuel A Barbieri
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引用次数: 0

摘要

背景:山番子的果实和种子提取物对几种癌症细胞具有显著的细胞毒性潜力。本研究评估了A.montana叶己烷提取物对胰岛素样生长因子-1(IGF-1)刺激下转移性乳腺癌症细胞的几种信号级联和基因表达的影响。方法:采用MTT法检测癌症细胞增殖情况。碘化丙啶染色和膜联蛋白V结合的流式细胞术分析用于测量细胞周期的进展和细胞凋亡的诱导。蛋白质表达和磷酸化通过蛋白质印迹分析来测定,以检测用A.montana叶己烷提取物处理后触发的潜在细胞机制。结果:A.montana叶片己烷(亚组分V)阻断了PI3K/mTOR信号通路的组成型刺激。这种抑制作用与细胞凋亡诱导有关,如膜联蛋白V和末端脱氧核苷酸转移酶dUTP缺口末端标记(TUNNEL)染色的阳性、胱天蛋白酶-3的激活和PPAR的切割所证明的。它还限制了调节增殖、存活、转移和血管生成的各种下游基因(即细胞周期蛋白D1、生存素、COX-2和VEGF)的表达。它增加了p53和p21的表达。有趣的是,我们还观察到,这种提取物阻断了AKT和ERK的激活,而不影响IGF-1受体的磷酸化和IGF-1刺激时Ras的激活。结论:A.montana叶(亚组分V)提取物通过PI3K/AKT/mTOR/S6K1途径对转移性MDA-MB-231乳腺癌症细胞具有选择性的抗增殖和促凋亡作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-proliferative effect of Annona extracts on breast cancer cells.

Backgorund: Fruits and seed extracts of Annona montana have significant cytotoxic potential in several cancer cells. This study evaluates the effect of A. montana leaves hexane extract on several signaling cascades and gene expression in metastatic breast cancer cells upon insulin-like growth factor-1 (IGF-1) stimulation.

Methods: MTT assay was performed to determine the proliferation of cancer cells. Propidium iodide staining and flow cytometry analysis of Annexin V binding was utilized to measure the progression of the cell cycle and the induction of apoptosis. Protein expression and phosphorylation were determined by western blotting analysis to examine the underlying cellular mechanism triggered upon treatment with A. montana leaves hexane extract.

Results: A. montana leaves hexane (sub-fraction V) blocked the constitutive stimulation of the PI3K/mTOR signaling pathways. This inhibitory effect was associated with apoptosis induction as evidenced by the positivity with Annexin V and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNNEL) staining, activation of caspase-3, and cleavage of PPAR. It also limited the expression of various downstream genes that regulate proliferation, survival, metastasis, and angiogenesis (i.e., cyclin D1, survivin, COX-2, and VEGF). It increased the expression of p53 and p21. Interestingly, we also observed that this extract blocked the activation of AKT and ERK without affecting the phosphorylation of the IGF-1 receptor and activation of Ras upon IGF-1 stimulation.

Conclusion: Our study indicates that A. montana leaves (sub-fraction V) extract exhibits a selective anti-proliferative and proapoptotic effect on the metastatic MDA-MB-231 breast cancer cells through the involvement of PI3K/AKT/mTOR/S6K1 pathways.

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来源期刊
Biocell
Biocell 生物-生物学
CiteScore
1.50
自引率
16.70%
发文量
259
审稿时长
>12 weeks
期刊介绍: BIOCELL welcomes Research articles and Review papers on structure, function and macromolecular organization of cells and cell components, focusing on cellular dynamics, motility and differentiation, particularly if related to cellular biochemistry, molecular biology, immunology, neurobiology, and on the suborganismal and organismal aspects of Vertebrate Reproduction and Development, Invertebrate Biology and Plant Biology.
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