Biological Bulletin最新文献_第7页

Multigenerational Life-History Responses to pH in Distinct Populations of the Copepod Tigriopus californicus 加利福尼亚虎足类不同种群对pH值的多代生活史响应

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-04-01 DOI: 10.1086/719573

A. Liguori

{"title":"Multigenerational Life-History Responses to pH in Distinct Populations of the Copepod Tigriopus californicus","authors":"A. Liguori","doi":"10.1086/719573","DOIUrl":"https://doi.org/10.1086/719573","url":null,"abstract":"Intertidal zones are highly dynamic and harsh habitats: organisms that persist there must face many stressors, including drastic changes in seawater pH, which can be strongly influenced by biological processes. Coastal ecosystems are heterogeneous in space and time, and populations can be exposed to distinct selective pressures and evolve different capacities for acclimation to changes in pH. Tigriopus californicus is a harpacticoid copepod found in high-shore rock pools on the west coast of North America. It is a model system for studying population dynamics in diverse environments, but little is known about its responses to changes in seawater pH. I quantified the effects of pH on the survivorship, fecundity, and development of four T. californicus populations from San Juan Island, Washington, across three generations. For all populations and generations, copepod cultures had lower survivorship and delayed development under extended exposure to higher pH treatments (pH 7.5 and pH 8.0), whereas cultures maintained in lower pH (7.0) displayed stable population growth over time. Reciprocal transplants between treatments demonstrated that these pH effects were reversible. Life histories were distinct between populations, and there were differences in the magnitudes of pH effects on development and culture growth that persisted through multiple generations. These results suggest that T. californicus might not have the generalist physiology that might be expected of an intertidal species, and it could be adapted to lower average pH conditions than those that occur in adjacent open waters.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":"242 1","pages":"97 - 117"},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42672670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Exploring the Candidate Terminal Glycan Profile in Neural Regeneration of the Sea Urchin Paracentrotus lividus, Using Lectin Blotting and Mass Spectrometry 利用凝集素印迹和质谱技术研究海胆神经再生的候选末端聚糖谱

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-03-09 DOI: 10.1086/718776

Ramiz Demir, Umut Şahar, Remziye Deveci

{"title":"Exploring the Candidate Terminal Glycan Profile in Neural Regeneration of the Sea Urchin Paracentrotus lividus, Using Lectin Blotting and Mass Spectrometry","authors":"Ramiz Demir, Umut Şahar, Remziye Deveci","doi":"10.1086/718776","DOIUrl":"https://doi.org/10.1086/718776","url":null,"abstract":"Glycans are expressed as conjugates of glycoproteins, glycolipids, and proteoglycans. The huge diversity of glycans on glycoconjugates contributes to many biological processes, from glycan-based molecular recognition to developmental events, such as regeneration in the nervous system. Echinoderms, which have a close phylogenetic relationship with chordates, are an important group of marine invertebrates for body regeneration. Although many major roles of glycans on glycoconjugates are known, their role in the glycosylation profile of the nervous system in sea urchins is poorly understood. In this study, we aimed to determine the terminal glycan profile by lectin blotting and to quantify sialic acids by the capillary liquid chromatography electrospray ionization tandem mass spectrometry system in the nervous tissue of the sea urchin Paracentrotus lividus. We determined the N-acetyl-D-glucosamine, mannose, and sialic acids (mainly α2,3 linked) by lectin blotting and five types of sialic acids (N-glycolylneuraminic acid, N-acetylneuraminic acid, 9-O-acetyl-N-alycolylneuraminic acid, 5-N-acetyl-9-O-acetyl-N-acetylneuraminic acid, and di-O-acetylated-N-alycolylneuraminic acid) by capillary liquid chromatography electrospray ionization tandem mass spectrometry. This potential first description of the terminal glycan profile in the nervous system of the sea urchin is expected to help us understand its role in nervous system development and regeneration.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":"242 1","pages":"118 - 126"},"PeriodicalIF":1.6,"publicationDate":"2022-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42725523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gamma Capsulotomy: A Valid Option for Refractory Obsessive Compulsive Disorder. 伽马胶囊切除术：治疗难治性强迫症的有效方法。

IF 0.8 4区生物学

Biological Bulletin Pub Date : 2022-02-17 eCollection Date: 2022-04-01 DOI: 10.1055/s-0041-1742119

Manjul Tripathi, Nishanth Sadashiva

引用次数: 0

Factors Modulating the Female Reproductive Performance of the Fiddler Crab Leptuca uruguayensis with Short Reproductive Season 短繁殖季招潮蟹雌性繁殖性能的调节因素

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-02-01 DOI: 10.1086/718263

Agustina Marciano, L. L. Greco, K. D. Colpo

{"title":"Factors Modulating the Female Reproductive Performance of the Fiddler Crab Leptuca uruguayensis with Short Reproductive Season","authors":"Agustina Marciano, L. L. Greco, K. D. Colpo","doi":"10.1086/718263","DOIUrl":"https://doi.org/10.1086/718263","url":null,"abstract":"This study aimed to evaluate the factors modulating the female reproductive performance of the fiddler crab Leptuca uruguayensis (Nobili, 1901) during the short reproductive season of a temperate population. We proposed two modulating factors: the age of females (young and old) and the periods of the reproductive season (beginning, middle, and end); we then evaluated the fecundity, reproductive output, egg volume, and biochemical composition of eggs. The fecundity of L. uruguayensis was affected by the size of females, a variable related to their age. Although young females showed lower fecundity, the reproductive output was not affected by the age or by the periods of the reproductive season, suggesting a constant reproductive effort, proportional to female size. The egg volume decreased, and carotenoid content increased at the end of the season for both female ages, probably as a consequence of variations in food availability and changes in the breeding strategies during the season. However, the content of protein and lipids in the egg clutches decreased at the end of the season only in old females spawning for the second time in the season. The main differences in the reproductive parameters were recorded between the beginning and the end of the reproductive season, probably because in these periods females exclusively use one of the breeding strategies. Finally, we determined that both factors, that is, female age and the periods of the short reproductive season, can modulate the reproductive performance of L. uruguayensis in temperate estuaries.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":"242 1","pages":"16 - 26"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42293402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Influence of Natural Barriers on the Amphidromous Shrimp Potimirim brasiliana (Caridea, Atyidae) from Two Rivers in Southeastern Brazil 自然屏障对巴西东南部两河两栖对虾(虾亚目，虾科)的影响

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-02-01 DOI: 10.1086/718590

Rafael de Carvalho Santos, J. A. Pantaleão, J. N. Teles, N. F. França, Jaqueline Roberta Pereira da Costa, H. Cabral, L. Pardo, F. Mantelatto

{"title":"The Influence of Natural Barriers on the Amphidromous Shrimp Potimirim brasiliana (Caridea, Atyidae) from Two Rivers in Southeastern Brazil","authors":"Rafael de Carvalho Santos, J. A. Pantaleão, J. N. Teles, N. F. França, Jaqueline Roberta Pereira da Costa, H. Cabral, L. Pardo, F. Mantelatto","doi":"10.1086/718590","DOIUrl":"https://doi.org/10.1086/718590","url":null,"abstract":"The migratory behavior of freshwater shrimps may be affected by natural barriers in limnetic environments. This study evaluated the river areas separated by natural barriers, such as waterfalls, which affect the amphidromous shrimps’ (Potimirim brasiliana) population features and reproductive aspects. Results indicate that in the Félix and Prumirim Rivers from southeastern Brazil shrimps show few differences in sampling areas, and these differences may not be causally related to the waterfalls. This is demonstrated by the absence of a pattern in the size and sex ratio in each area and the absence of a significant difference in most reproductive aspects. The presence of juveniles and reproductive individuals in all sampling areas strongly indicates a constant migration along them in both rivers, indicating that all individuals evaluated correspond to one single patchy population structure for each river. This migration conducted by P. brasiliana, such as its crawling behavior, demonstrated that it would be important to maintain the minimum number of individuals flowing between the different river sampling areas in this shrimp group. Thus, based on a helpful model observed in P. brasiliana, the results help us understand how natural barriers may affect the populations of amphidromous shrimp and how the migration behavior up- and downstream can help sustain the population. This premise can help future construction decisions and impacts of unnatural barriers, such as dams.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":"242 1","pages":"27 - 39"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48334948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Functional Characterization of TRPV-Like Ion Channels Involved in Nematocyst Discharge from the Sea Anemone Diadumene lineata 参与海葵刺丝囊排出的trpv样离子通道的功能表征

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-02-01 DOI: 10.1086/717902

Alice Nam, Selorm Quarshie, M. Kimble, D. Hessinger

{"title":"Functional Characterization of TRPV-Like Ion Channels Involved in Nematocyst Discharge from the Sea Anemone Diadumene lineata","authors":"Alice Nam, Selorm Quarshie, M. Kimble, D. Hessinger","doi":"10.1086/717902","DOIUrl":"https://doi.org/10.1086/717902","url":null,"abstract":"Cnidarians require mechanical stimuli to trigger nematocyst discharge and initiate feeding behaviors. The interval from triggering stimulus to response is tens of microseconds, making it likely that mechanically gated ion channels trigger nematocyst discharge. Because many transient receptor potential channels are mechanically gated, we hypothesized that nematocyst discharge involves transient receptor potential channels. We therefore tested various transient receptor potential channel inhibitors to determine whether they inhibit nematocyst discharge and prey killing in the acontiate sea anemone (Actinaria) Diadumene lineata (a.k.a. Haliplanella luciae). Three types of cnidocyte supporting cell complexes regulate nematocyst discharge in anemones: Types C, B, and A. Discharge from Type Cs is directly triggered by stimulation of contact-sensitive mechanoreceptors, while Type Bs require activation of chemoreceptors from prey-derived N-acetylated sugars to sensitize contact-sensitive mechanoreceptors. In Type As, activated chemoreceptors tune vibration-sensitive mechanoreceptors that predispose contact-sensitive mechanoreceptors for triggering. The non-selective transient receptor potential channel blockers lanthanum and gadolinium dose-dependently inhibited about 80% of prey killing and all nematocyst discharge from Type Bs and Type Cs, but not Type As. The selective transient receptor potential vanilloid 4 (TRPV4) blocker GSK2193874 inhibited Type As and Type Bs. However, the selective TRPV4 blockers HC-067047 and RN-1734 inhibited only Type As. Thus, three TRPV4-selective blockers implicate TRPV-like involvement in discharge from Type As, whereas GSK2193874 also affected Type Bs. Our results suggest that a TRPV-like homolog plays an essential role in nematocyst-mediated prey killing from Type As, whereas other transient receptor potential channels are likely involved in discharge from Type B and C cnidocyte supporting cell complexes.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":"242 1","pages":"48 - 61"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43603986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth and Cyclin-E Expression in the Stony Coral Species Orbicella faveolata Post-Microfragmentation 石珊瑚小球藻微粉碎后的生长和细胞周期蛋白E的表达

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-02-01 DOI: 10.1086/717926

D. Soper, N. Villafranca, J. P. Dieffenthaller, M. Dieffenthaller, H. Tompkins, M. Weglarz, D. Vaughan, S. B. Hamlyn, D. Stenesen

{"title":"Growth and Cyclin-E Expression in the Stony Coral Species Orbicella faveolata Post-Microfragmentation","authors":"D. Soper, N. Villafranca, J. P. Dieffenthaller, M. Dieffenthaller, H. Tompkins, M. Weglarz, D. Vaughan, S. B. Hamlyn, D. Stenesen","doi":"10.1086/717926","DOIUrl":"https://doi.org/10.1086/717926","url":null,"abstract":"Coral growth is critical to reef health, resilience under rapidly changing environmental conditions, and restoration efforts. Although fragmenting coral has been occurring for many years in an effort to restore reefs, recently it was discovered that microfragmenting, the process of cutting one piece of coral into many small pieces (about three to five polyps), induces exponential growth. Our study investigates the process by which microfragments of nine different genotypes from the stony coral species Orbicella faveolata grow and exhibit Cyclin-E expression. Microfragments were examined by using a high-powered dissecting microscope with a camera to document the precise areas of tissue exhibiting exponential growth. We found that new polyp formation occurs only on the microfragment edges and that edge polyp growth rates varied between different genotypes. We then extracted tissue from both the edge and the center of five genotypes for genetic analysis. We chose to analyze Cyclin-E expression because it is involved with stimulating mitotic division and is a conserved signaling pathway that is known to exist in Drosophila, mammals, and Cnidaria. Two primers for Cyclin-E were utilized to examine the level of expression for center and edge tissue. We found that Cyclin-E is expressed differentially between O. faveolata polyps, with a tendency for increased expression of the Cyclin-E in edge versus center tissue in each of five genotypes, although this result was not significant. Despite consistently higher levels of Cyclin-E expression within an organism’s edge tissue, genotypes varied significantly in the degree of increased expression. This variation positively correlated with growth rate, suggesting the potential for molecular selection in aid of more rapid reef restoration. Future work will focus on deciphering the specific growth pathways involved in microfragmented coral growth and analyzing expression patterns in injured tissues.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":"242 1","pages":"40 - 47"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42144860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Scope for Developmental Plasticity of Feeding Larvae of a Holothuroid, Contrasted with Other Echinoderm Larvae 食虫幼虫与其他棘皮动物幼虫发育可塑性的比较

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-01-11 DOI: 10.1086/717157

R. R. Strathmann

{"title":"Scope for Developmental Plasticity of Feeding Larvae of a Holothuroid, Contrasted with Other Echinoderm Larvae","authors":"R. R. Strathmann","doi":"10.1086/717157","DOIUrl":"https://doi.org/10.1086/717157","url":null,"abstract":"Feeding larvae of echinoderms appear to differ in scope for adaptive developmental plasticity in response to food. Extension of the ciliary band on narrow arms supported by skeletal rods, as in echinoid and ophiuroid larvae, may enable a greater increase in maximum clearance rate per cell added, conferring greater advantages from developing longer ciliary bands when food is scarce. Formation of the juvenile mouth and water vascular system at a new site, as in echinoid and asteroid larvae, permits extensive growth of the juvenile rudiment during larval feeding, with advantages from earlier or more growth of the rudiment when food is abundant. In contrast, plasticity in storage of nutrients is unrelated to the form of the ciliary band or the site of formation of the juvenile’s mouth. Feeding larvae (auriculariae) of holothuroids lack arms supported by skeletal rods and formation of the mouth at a new site but as a unique feature store nutrients in hyaline spheres. In this study, more food for auriculariae of Apostichopus californicus resulted in juveniles (pentactulae) with longer and wider bodies and larger hyaline spheres, but effects of food supply on the size of most body parts of auriculariae were small. Auriculariae with more food developed relatively larger stomachs and larger posterior hyaline spheres, indications of greater nutrient storage. Auriculariae with less food developed relatively wider mouths and differed in some exterior dimensions, which might enhance the capture of food. Plasticity is limited in rudiment development and perhaps in structures for feeding, but plasticity in nutrient storage can provide advantageous compromises between duration of growth as a feeding larva and the condition of juveniles formed at metamorphosis.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":"242 1","pages":"1 - 15"},"PeriodicalIF":1.6,"publicationDate":"2022-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43258417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the Effects of Symbiosis and Temperature on Coral Calcification 揭示共生和温度对珊瑚钙化的影响

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-01-10 DOI: 10.1086/716711

Zoe Dellaert, P. Vargas, P. L. La Riviere, Loretta M. Roberson

{"title":"Uncovering the Effects of Symbiosis and Temperature on Coral Calcification","authors":"Zoe Dellaert, P. Vargas, P. L. La Riviere, Loretta M. Roberson","doi":"10.1086/716711","DOIUrl":"https://doi.org/10.1086/716711","url":null,"abstract":"We tested the impact of temperature and symbiont state on calcification in corals, using the facultatively symbiotic coral Astrangia poculata as a model system. Symbiotic and aposymbiotic colonies of A. poculata were reared in 15, 20, and 27 °C conditions. We used scanning electron microscopy to quantify how these physiological and environmental conditions impact skeletal structure. Buoyant weight data over time revealed that temperature significantly affects calcification rates. Scanning electron microscopy of A. poculata skeletons showed that aposymbiotic colonies appear to have a lower density of calcium carbonate in actively growing septal spines. We describe a novel approach to analyze the roughness and texture of scanning electron microscopy images. Quantitative analysis of the roughness of septal spines revealed that aposymbiotic colonies have a rougher surface than symbiotic colonies in tropical conditions (27 °C). This trend reversed at 15 °C, a temperature at which the symbionts of A. poculata may exhibit parasitic properties. Analysis of surface texture patterns showed that temperature impacts the spatial variance of crystals on the spine surface. Few published studies have examined the skeleton of A. poculata by using scanning electron microscopy. Our approach provides a way to study detailed changes in skeletal microstructure in response to environmental parameters and can serve as a proxy for more expensive and time-consuming analyses. Utilizing a facultatively symbiotic coral that is native to both temperate and tropical regions provides new insights into the impact of both symbiosis and temperature on calcification in corals.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":"242 1","pages":"62 - 73"},"PeriodicalIF":1.6,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46618671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

About the Cover. 关于封面。

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2021-12-30 DOI: 10.7748/ns.23.2.62.s60

Tabi Crentsil

引用次数: 0