Biological Bulletin最新文献_第6页

Ecological Review of the Ciona Species Complex Ciona物种复合体的生态学研究进展

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-04-01 DOI: 10.1086/719476

Emmerson R. Wilson, Kieran J. Murphy, R. Wyeth

{"title":"Ecological Review of the Ciona Species Complex","authors":"Emmerson R. Wilson, Kieran J. Murphy, R. Wyeth","doi":"10.1086/719476","DOIUrl":"https://doi.org/10.1086/719476","url":null,"abstract":"The set of four closely related solitary ascidians Ciona spp. were once considered a single cosmopolitan species, Ciona intestinalis, but are now recognized as genetically and morphologically distinct species. The possibility of ecological differences between the species was not widely considered in studies preceding the schism of Ciona spp. Consequently, there may be an over-generalization of the ecology of Ciona spp., with potential implications for the broad range of studies targeting these species, encompassing the evolution, development, genomics, and invasion biology of Ciona spp. We completed a comprehensive review of the ecology of Ciona spp. to establish the similarities and differences between the widely distributed Ciona robusta and C. intestinalis (and what little is known of the two other species, Ciona sp. C and Ciona sp. D). When necessary, we used study locations and the species’ geographic ranges to infer the species in each study in the review. As expected, ecological similarities are the norm between the two species, spanning both abiotic and biotic interactions. However, there are also important differences that have potential implications for other aspects of the biology of Ciona spp. For example, differences in temperature and salinity tolerances likely correspond with the disparities in the geographic distribution of the species. Asymmetries in topics studied in each species diminish our ability to fully compare several aspects of the ecology of Ciona spp. and are priority areas for future research. We anticipate that our clarification of common and unique aspects of each species’ ecology will help to provide context for future research in many aspects of the biology of Ciona spp.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46587435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Molecular Characterization of the Cytochrome P450 Epoxidase (CYP15) in the Swimming Crab Portunus trituberculatus and Its Putative Roles in Methyl Farnesoate Metabolism 三瘤梭子蟹细胞色素P450环氧化酶(CYP15)的分子特征及其在法诺糖酸甲酯代谢中的作用

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-04-01 DOI: 10.1086/719047

Shisheng Tu, Ping Tuo, Dongjie Xu, Zhenya Wang, Mengen Wang, Xi Xie, Dongfa Zhu

{"title":"Molecular Characterization of the Cytochrome P450 Epoxidase (CYP15) in the Swimming Crab Portunus trituberculatus and Its Putative Roles in Methyl Farnesoate Metabolism","authors":"Shisheng Tu, Ping Tuo, Dongjie Xu, Zhenya Wang, Mengen Wang, Xi Xie, Dongfa Zhu","doi":"10.1086/719047","DOIUrl":"https://doi.org/10.1086/719047","url":null,"abstract":"CYP15, which encodes a microsomal cytochrome P450 enzyme, could be involved in juvenile hormone biosynthesis in insects. In this study, a full-length cDNA of CYP15 was cloned from the swimming crab Portunus trituberculatus. This PtCYP15 amino acid sequence contains six conserved domains, which is a typical feature of the cytochrome P450 family. Phylogenetic tree analysis results showed that PtCYP15 clusters in a single branch of crustacean species, suggesting that CYP15 may be more widely present in crustaceans. The PtCYP15 mRNA has a broad pattern of tissue expression in P. trituberculatus, including high levels of expression in the hepatopancreas of both sexes and in the ovary of female crabs. During ovarian development stages, PtCYP15 mRNA is highly expressed in stages I and II and less so in stages III and IV in the hepatopancreas and the ovary of the female crabs. These expression profiles are opposite those of methyl farnesoate in hemolymph, suggesting that PtCYP15 might be involved in methyl farnesoate metabolism. In vitro studies show that only methyl farnesoate upregulated vitellogenin expression in the hepatopancreas, suggesting that methyl farnesoate might be the equivalent of juvenile hormone III in crustaceans. Methyl farnesoate treatment increased levels of PtCYP15 in explants of the hepatopancreas and ovary, while juvenile hormone III treatment reduced levels of PtCYP15 mRNA in ovary explants, suggesting that PtCYP15 might be involved in degrading methyl farnesoate. Furthermore, PtCYP15 mRNA expression levels were inhibited by adding juvenile hormone III to ovary explants. These findings provide foundational information for future research on methyl farnesoate metabolism in crustaceans.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41655205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Monogamy in the Burrowing Shrimp Axianassa australis Rodrigues & Shimizu, 1992 (Decapoda, Gebiidea, Axianassidae) 穴虾的一夫一妻制(十足目，虾总科，穴虾科)

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-04-01 DOI: 10.1086/719408

P. Hernáez, R. Mugnai, J. F. Souza-Filho, M. Pinheiro

{"title":"Monogamy in the Burrowing Shrimp Axianassa australis Rodrigues & Shimizu, 1992 (Decapoda, Gebiidea, Axianassidae)","authors":"P. Hernáez, R. Mugnai, J. F. Souza-Filho, M. Pinheiro","doi":"10.1086/719408","DOIUrl":"https://doi.org/10.1086/719408","url":null,"abstract":"Our knowledge of the mating systems in burrowing shrimps (infraorders Axiidea and Gebiidea) is still rather limited. Here we describe the burrow use pattern, sex ratio, and sexual dimorphism of the burrowing shrimp Axianassa australis to test for monogamy, considering that monogamous species live in heterosexual pairs and exhibit a low degree of sexual dimorphism. To this end, a total of 226 individuals of A. australis were collected from the northeast region of Brazil. Our results showed that A. australis inhabited its burrows mainly as pairs, most of which were male-female pairs. In agreement with the expectations, specimens of A. australis were found dwelling as heterosexual pairs more frequently than expected by chance alone. The presence of ovigerous females was associated with the burrow occupation; that is, brooding females were more frequently observed in male-female combinations than solitarily. Also supporting theoretical considerations, we did not observe sexual dimorphism in body size between males and females of the population and the different categories of the burrow occupation. Conversely, sexual dimorphism in cheliped size was evident in the population, with larger chelipeds in males than in females. This observation agrees with that reported for most burrowing shrimps in which male-male competition is the main evolutionary force of sexual selection. The observations above favor the hypothesis that A. australis is primarily monogamous, with a small fraction of the males moderately promiscuous.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43362579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Multigenerational Life-History Responses to pH in Distinct Populations of the Copepod Tigriopus californicus 加利福尼亚虎足类不同种群对pH值的多代生活史响应

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-04-01 DOI: 10.1086/719573

A. Liguori

{"title":"Multigenerational Life-History Responses to pH in Distinct Populations of the Copepod Tigriopus californicus","authors":"A. Liguori","doi":"10.1086/719573","DOIUrl":"https://doi.org/10.1086/719573","url":null,"abstract":"Intertidal zones are highly dynamic and harsh habitats: organisms that persist there must face many stressors, including drastic changes in seawater pH, which can be strongly influenced by biological processes. Coastal ecosystems are heterogeneous in space and time, and populations can be exposed to distinct selective pressures and evolve different capacities for acclimation to changes in pH. Tigriopus californicus is a harpacticoid copepod found in high-shore rock pools on the west coast of North America. It is a model system for studying population dynamics in diverse environments, but little is known about its responses to changes in seawater pH. I quantified the effects of pH on the survivorship, fecundity, and development of four T. californicus populations from San Juan Island, Washington, across three generations. For all populations and generations, copepod cultures had lower survivorship and delayed development under extended exposure to higher pH treatments (pH 7.5 and pH 8.0), whereas cultures maintained in lower pH (7.0) displayed stable population growth over time. Reciprocal transplants between treatments demonstrated that these pH effects were reversible. Life histories were distinct between populations, and there were differences in the magnitudes of pH effects on development and culture growth that persisted through multiple generations. These results suggest that T. californicus might not have the generalist physiology that might be expected of an intertidal species, and it could be adapted to lower average pH conditions than those that occur in adjacent open waters.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42672670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Carcinoecium-Forming Sea Anemone Stylobates calcifer sp. nov. (Cnidaria, Actiniaria, Actiniidae) from the Japanese Deep-Sea Floor: A Taxonomical Description with Its Ecological Observations 日本深海海葵styloates calcifer sp. 11(刺胞目，Actiniaria, actinidae):分类描述及其生态观察

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-04-01 DOI: 10.1086/719160

Akihiro Yoshikawa, Takato Izumi, Takeya Moritaki, Taeko Kimura, Kensuke Yanagi

{"title":"Carcinoecium-Forming Sea Anemone Stylobates calcifer sp. nov. (Cnidaria, Actiniaria, Actiniidae) from the Japanese Deep-Sea Floor: A Taxonomical Description with Its Ecological Observations","authors":"Akihiro Yoshikawa, Takato Izumi, Takeya Moritaki, Taeko Kimura, Kensuke Yanagi","doi":"10.1086/719160","DOIUrl":"https://doi.org/10.1086/719160","url":null,"abstract":"Here we describe Stylobates calcifer sp. nov. (Cnidaria, Actiniaria, Actiniidae), a new carcinoecium-forming sea anemone from the deep-sea floor of Japan. Stylobates produces a carcinoecium that thinly covers the snail shells inhabited by host hermit crabs Pagurodofleinia doederleini. The new species is distinct from other species by the shape of the marginal sphincter muscle, the distribution of cnidae, the direction of the oral disk, and host association. The species’ novelty is supported by the data of its mitochondrial genes 12S, 16S, and COIII and nuclear genes 18S and 28S. Also, we conducted behavioral observation of this new species, focusing on the feeding behavior and interaction with the specific host hermit crab. Our observations suggest that this sea anemone potentially feeds on the suspended particulate organic matter from the water column or the food residuals of hermit crabs. When the host’s shell changed, intensive manipulation for transference of S. calcifer sp. nov. was recorded. However, although the hermit crab detached and transferred the sea anemone to the new shell after shell change, the sea anemone did not exhibit active or cooperative participation. Our data suggest that the sea anemone may not produce a carcinoecium synchronously to its host’s growth, contrary to the anecdotal assumption about carcinoecium-forming sea anemones. Conversely, the host hermit crab’s growth may not depend entirely on the carcinoecium produced by the sea anemone. This study is perhaps the first observation of the behavioral interaction of the rarely studied carcinoecium-forming mutualism in the deep sea.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43887141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Exploring the Candidate Terminal Glycan Profile in Neural Regeneration of the Sea Urchin Paracentrotus lividus, Using Lectin Blotting and Mass Spectrometry 利用凝集素印迹和质谱技术研究海胆神经再生的候选末端聚糖谱

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-03-09 DOI: 10.1086/718776

Ramiz Demir, Umut Şahar, Remziye Deveci

{"title":"Exploring the Candidate Terminal Glycan Profile in Neural Regeneration of the Sea Urchin Paracentrotus lividus, Using Lectin Blotting and Mass Spectrometry","authors":"Ramiz Demir, Umut Şahar, Remziye Deveci","doi":"10.1086/718776","DOIUrl":"https://doi.org/10.1086/718776","url":null,"abstract":"Glycans are expressed as conjugates of glycoproteins, glycolipids, and proteoglycans. The huge diversity of glycans on glycoconjugates contributes to many biological processes, from glycan-based molecular recognition to developmental events, such as regeneration in the nervous system. Echinoderms, which have a close phylogenetic relationship with chordates, are an important group of marine invertebrates for body regeneration. Although many major roles of glycans on glycoconjugates are known, their role in the glycosylation profile of the nervous system in sea urchins is poorly understood. In this study, we aimed to determine the terminal glycan profile by lectin blotting and to quantify sialic acids by the capillary liquid chromatography electrospray ionization tandem mass spectrometry system in the nervous tissue of the sea urchin Paracentrotus lividus. We determined the N-acetyl-D-glucosamine, mannose, and sialic acids (mainly α2,3 linked) by lectin blotting and five types of sialic acids (N-glycolylneuraminic acid, N-acetylneuraminic acid, 9-O-acetyl-N-alycolylneuraminic acid, 5-N-acetyl-9-O-acetyl-N-acetylneuraminic acid, and di-O-acetylated-N-alycolylneuraminic acid) by capillary liquid chromatography electrospray ionization tandem mass spectrometry. This potential first description of the terminal glycan profile in the nervous system of the sea urchin is expected to help us understand its role in nervous system development and regeneration.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42725523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors Modulating the Female Reproductive Performance of the Fiddler Crab Leptuca uruguayensis with Short Reproductive Season 短繁殖季招潮蟹雌性繁殖性能的调节因素

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-02-01 DOI: 10.1086/718263

Agustina Marciano, L. L. Greco, K. D. Colpo

{"title":"Factors Modulating the Female Reproductive Performance of the Fiddler Crab Leptuca uruguayensis with Short Reproductive Season","authors":"Agustina Marciano, L. L. Greco, K. D. Colpo","doi":"10.1086/718263","DOIUrl":"https://doi.org/10.1086/718263","url":null,"abstract":"This study aimed to evaluate the factors modulating the female reproductive performance of the fiddler crab Leptuca uruguayensis (Nobili, 1901) during the short reproductive season of a temperate population. We proposed two modulating factors: the age of females (young and old) and the periods of the reproductive season (beginning, middle, and end); we then evaluated the fecundity, reproductive output, egg volume, and biochemical composition of eggs. The fecundity of L. uruguayensis was affected by the size of females, a variable related to their age. Although young females showed lower fecundity, the reproductive output was not affected by the age or by the periods of the reproductive season, suggesting a constant reproductive effort, proportional to female size. The egg volume decreased, and carotenoid content increased at the end of the season for both female ages, probably as a consequence of variations in food availability and changes in the breeding strategies during the season. However, the content of protein and lipids in the egg clutches decreased at the end of the season only in old females spawning for the second time in the season. The main differences in the reproductive parameters were recorded between the beginning and the end of the reproductive season, probably because in these periods females exclusively use one of the breeding strategies. Finally, we determined that both factors, that is, female age and the periods of the short reproductive season, can modulate the reproductive performance of L. uruguayensis in temperate estuaries.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42293402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Influence of Natural Barriers on the Amphidromous Shrimp Potimirim brasiliana (Caridea, Atyidae) from Two Rivers in Southeastern Brazil 自然屏障对巴西东南部两河两栖对虾(虾亚目，虾科)的影响

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-02-01 DOI: 10.1086/718590

Rafael de Carvalho Santos, J. A. Pantaleão, J. N. Teles, N. F. França, Jaqueline Roberta Pereira da Costa, H. Cabral, L. Pardo, F. Mantelatto

{"title":"The Influence of Natural Barriers on the Amphidromous Shrimp Potimirim brasiliana (Caridea, Atyidae) from Two Rivers in Southeastern Brazil","authors":"Rafael de Carvalho Santos, J. A. Pantaleão, J. N. Teles, N. F. França, Jaqueline Roberta Pereira da Costa, H. Cabral, L. Pardo, F. Mantelatto","doi":"10.1086/718590","DOIUrl":"https://doi.org/10.1086/718590","url":null,"abstract":"The migratory behavior of freshwater shrimps may be affected by natural barriers in limnetic environments. This study evaluated the river areas separated by natural barriers, such as waterfalls, which affect the amphidromous shrimps’ (Potimirim brasiliana) population features and reproductive aspects. Results indicate that in the Félix and Prumirim Rivers from southeastern Brazil shrimps show few differences in sampling areas, and these differences may not be causally related to the waterfalls. This is demonstrated by the absence of a pattern in the size and sex ratio in each area and the absence of a significant difference in most reproductive aspects. The presence of juveniles and reproductive individuals in all sampling areas strongly indicates a constant migration along them in both rivers, indicating that all individuals evaluated correspond to one single patchy population structure for each river. This migration conducted by P. brasiliana, such as its crawling behavior, demonstrated that it would be important to maintain the minimum number of individuals flowing between the different river sampling areas in this shrimp group. Thus, based on a helpful model observed in P. brasiliana, the results help us understand how natural barriers may affect the populations of amphidromous shrimp and how the migration behavior up- and downstream can help sustain the population. This premise can help future construction decisions and impacts of unnatural barriers, such as dams.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48334948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Functional Characterization of TRPV-Like Ion Channels Involved in Nematocyst Discharge from the Sea Anemone Diadumene lineata 参与海葵刺丝囊排出的trpv样离子通道的功能表征

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-02-01 DOI: 10.1086/717902

Alice Nam, Selorm Quarshie, M. Kimble, D. Hessinger

{"title":"Functional Characterization of TRPV-Like Ion Channels Involved in Nematocyst Discharge from the Sea Anemone Diadumene lineata","authors":"Alice Nam, Selorm Quarshie, M. Kimble, D. Hessinger","doi":"10.1086/717902","DOIUrl":"https://doi.org/10.1086/717902","url":null,"abstract":"Cnidarians require mechanical stimuli to trigger nematocyst discharge and initiate feeding behaviors. The interval from triggering stimulus to response is tens of microseconds, making it likely that mechanically gated ion channels trigger nematocyst discharge. Because many transient receptor potential channels are mechanically gated, we hypothesized that nematocyst discharge involves transient receptor potential channels. We therefore tested various transient receptor potential channel inhibitors to determine whether they inhibit nematocyst discharge and prey killing in the acontiate sea anemone (Actinaria) Diadumene lineata (a.k.a. Haliplanella luciae). Three types of cnidocyte supporting cell complexes regulate nematocyst discharge in anemones: Types C, B, and A. Discharge from Type Cs is directly triggered by stimulation of contact-sensitive mechanoreceptors, while Type Bs require activation of chemoreceptors from prey-derived N-acetylated sugars to sensitize contact-sensitive mechanoreceptors. In Type As, activated chemoreceptors tune vibration-sensitive mechanoreceptors that predispose contact-sensitive mechanoreceptors for triggering. The non-selective transient receptor potential channel blockers lanthanum and gadolinium dose-dependently inhibited about 80% of prey killing and all nematocyst discharge from Type Bs and Type Cs, but not Type As. The selective transient receptor potential vanilloid 4 (TRPV4) blocker GSK2193874 inhibited Type As and Type Bs. However, the selective TRPV4 blockers HC-067047 and RN-1734 inhibited only Type As. Thus, three TRPV4-selective blockers implicate TRPV-like involvement in discharge from Type As, whereas GSK2193874 also affected Type Bs. Our results suggest that a TRPV-like homolog plays an essential role in nematocyst-mediated prey killing from Type As, whereas other transient receptor potential channels are likely involved in discharge from Type B and C cnidocyte supporting cell complexes.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43603986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth and Cyclin-E Expression in the Stony Coral Species Orbicella faveolata Post-Microfragmentation 石珊瑚小球藻微粉碎后的生长和细胞周期蛋白E的表达

IF 1.6 4区生物学

Biological Bulletin Pub Date : 2022-02-01 DOI: 10.1086/717926

D. Soper, N. Villafranca, J. P. Dieffenthaller, M. Dieffenthaller, H. Tompkins, M. Weglarz, D. Vaughan, S. B. Hamlyn, D. Stenesen

{"title":"Growth and Cyclin-E Expression in the Stony Coral Species Orbicella faveolata Post-Microfragmentation","authors":"D. Soper, N. Villafranca, J. P. Dieffenthaller, M. Dieffenthaller, H. Tompkins, M. Weglarz, D. Vaughan, S. B. Hamlyn, D. Stenesen","doi":"10.1086/717926","DOIUrl":"https://doi.org/10.1086/717926","url":null,"abstract":"Coral growth is critical to reef health, resilience under rapidly changing environmental conditions, and restoration efforts. Although fragmenting coral has been occurring for many years in an effort to restore reefs, recently it was discovered that microfragmenting, the process of cutting one piece of coral into many small pieces (about three to five polyps), induces exponential growth. Our study investigates the process by which microfragments of nine different genotypes from the stony coral species Orbicella faveolata grow and exhibit Cyclin-E expression. Microfragments were examined by using a high-powered dissecting microscope with a camera to document the precise areas of tissue exhibiting exponential growth. We found that new polyp formation occurs only on the microfragment edges and that edge polyp growth rates varied between different genotypes. We then extracted tissue from both the edge and the center of five genotypes for genetic analysis. We chose to analyze Cyclin-E expression because it is involved with stimulating mitotic division and is a conserved signaling pathway that is known to exist in Drosophila, mammals, and Cnidaria. Two primers for Cyclin-E were utilized to examine the level of expression for center and edge tissue. We found that Cyclin-E is expressed differentially between O. faveolata polyps, with a tendency for increased expression of the Cyclin-E in edge versus center tissue in each of five genotypes, although this result was not significant. Despite consistently higher levels of Cyclin-E expression within an organism’s edge tissue, genotypes varied significantly in the degree of increased expression. This variation positively correlated with growth rate, suggesting the potential for molecular selection in aid of more rapid reef restoration. Future work will focus on deciphering the specific growth pathways involved in microfragmented coral growth and analyzing expression patterns in injured tissues.","PeriodicalId":55376,"journal":{"name":"Biological Bulletin","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42144860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1