Biopreservation and Biobanking最新文献

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Trehalose Cryopreservation of Human Mesenchymal Stem Cells from Cord Tissue. 人脐带间充质干细胞的海藻糖低温保存。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-26 DOI: 10.1089/bio.2024.0025
Nuria Izaguirre-Pérez, Gertrudis Ligero, Paula Alba Aguilar-Solana, José Antonio Carrillo-Ávila, Carmen Ruth Rodriguez-Reyes, Ida Biunno, Rocío Aguilar-Quesada, Purificación Catalina
{"title":"Trehalose Cryopreservation of Human Mesenchymal Stem Cells from Cord Tissue.","authors":"Nuria Izaguirre-Pérez, Gertrudis Ligero, Paula Alba Aguilar-Solana, José Antonio Carrillo-Ávila, Carmen Ruth Rodriguez-Reyes, Ida Biunno, Rocío Aguilar-Quesada, Purificación Catalina","doi":"10.1089/bio.2024.0025","DOIUrl":"https://doi.org/10.1089/bio.2024.0025","url":null,"abstract":"<p><p>Adequate hypothermic storage of human mesenchymal stem cells (hMSCs) is of fundamental importance since they have been explored in several regenerative medicine initiatives. However, the actual clinical application of hMSCs necessitates hypothermic storage for long periods, a process that requires the use of non-toxic and efficient cryo-reagents capable of maintaining high viability and differentiating properties after thawing. Current cryopreservation methods are based on cryoprotectant agents (CPAs) containing dimethylsulphoxide (DMSO), which have been shown to be toxic for clinical applications. In this study, we describe a simple and effective trehalose (TRE)-based solution to cryo-store human umbilical cord-derived MSCs (UC-MSCs) in liquid nitrogen. Cells viability, identity, chromosomal stability, proliferative and migration capacity, and stress response were assessed after cryopreservation in TRE as CPA, testing different concentrations by itself or in combination with ethylene glycol (EG). Here we show that TRE-stored UC-MSCs provided lower cell recovery rates compared with DMSO-based solution, but maintained good functional properties, stability, and differentiating potential. The best cell recovery was obtained using 0.5 M TRE with 10% EG showing no differences in the osteogenic, adipogenic, and chondrogenic differentiation capacity. A second cycle of cryopreservation in this TRE-based solution had no additional impact on the viability and morphology, although slightly affected cell migration. Furthermore, the expression of the stress-related genes, <i>HSPA1A</i>, <i>SOD2</i>, <i>TP53</i>, <i>BCL-2</i>, and <i>BAX</i>, did not show a higher response in UC-MSCs cryopreserved in 0.5 M TRE + 10% EG compared with DMSO. Together these results, in addition to ascertained therapeutic properties of TRE, provide sufficient evidence to consider TRE-based medium as a low-cost and efficient solution for the storage of human UC-MSCs cells and potentially substitute DMSO-based cryo-reagents.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Data Governance and Distribution of Biobank: A Case from a Chinese Cancer Hospital. 生物样本库的数据治理与分布:以某肿瘤医院为例。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-13 DOI: 10.1089/bio.2024.0081
Jingjing Shi, Yan Guo, Na He, Wenbin Xia, Hongkun Liu, Haixin Li
{"title":"Data Governance and Distribution of Biobank: A Case from a Chinese Cancer Hospital.","authors":"Jingjing Shi, Yan Guo, Na He, Wenbin Xia, Hongkun Liu, Haixin Li","doi":"10.1089/bio.2024.0081","DOIUrl":"https://doi.org/10.1089/bio.2024.0081","url":null,"abstract":"<p><p><b><i>Objectives:</i></b> To facilitate the regionalization, specialization, and digitization of biobanks, three issues regarding data collection and application must be addressed (1) integration and distribution of data governance, (2) efficiency and efficacy of data governance, and (3) sustainability of data governance. <b><i>Methods:</i></b> We collaborated with stakeholders to identify priorities and assess infrastructure needs through the continuous evaluation and analysis of projects. We developed data management solutions, catalogs, and data models to optimize and support data collection, distribution, and application. Furthermore, ontologies were used to facilitate data integration from multiple sources, and Minimum Information About BIobank Data Sharing (MIABIS) was defined as accessible to all patients. To enhance data integrity, we conducted retrospective and prospective follow-up studies. <b><i>Results:</i></b> We completed infrastructure upgrades to match technical solutions and research demands. An information management software with six primary functional divisions was developed for data governance. We optimized the database structure and changed the biospecimen accumulation model from biospecimen-based to patient-centered and service-oriented. Subsequently, we specified 85 attributes of MIABIS to describe the biobank contents. A dual-pillar approach was adopted to expand the biobank's data in collaboration with other institutions, and MIABIS served as a bridge for both vertical and horizontal networks. From 2003 to 2021, we collected a total of 156,997 patient biospecimens/data from 20 cancer types, matching 53,113 cases from follow-up surveys. In addition, we supplied more than 40,000 biospecimens/data points for above 300 scientific research projects. <b><i>Conclusions:</i></b> An appropriate information platform for a biobank is fundamental to data collection, distribution, and application, particularly in the context of data-intensive research. We implemented a standardized scientific data structure to fulfill the research requirements. The sustainable development of a biobank depends on a scientific, standardized, and service-oriented data governance approach, along with the efficient utilization of emerging technologies.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bridging Financial Challenges in Young Biobanks-Funding Strategies from the Central Biobank Regensburg. 应对年轻生物银行的财务挑战--雷根斯堡中央生物银行的筹资策略。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-12 DOI: 10.1089/bio.2023.0129
Deborah Seidler, Lina Winter, Marie Karlíková, Ondrej Topolčan, Katja Steiger, Kateřina Nováková, Ralph Burkhardt, Tanja Niedermair, Christoph Brochhausen
{"title":"Bridging Financial Challenges in Young Biobanks-Funding Strategies from the Central Biobank Regensburg.","authors":"Deborah Seidler, Lina Winter, Marie Karlíková, Ondrej Topolčan, Katja Steiger, Kateřina Nováková, Ralph Burkhardt, Tanja Niedermair, Christoph Brochhausen","doi":"10.1089/bio.2023.0129","DOIUrl":"https://doi.org/10.1089/bio.2023.0129","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Developing a Short Course on the Ethical, Legal, and Social Issues of Biobanking Practice-A Participatory Action Research Study in South Africa. 在南非开发关于生物银行实践中的伦理、法律和社会问题的短期课程--参与式行动研究。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-01 Epub Date: 2024-07-08 DOI: 10.1089/bio.2023.0165
Shenuka Singh
{"title":"Developing a Short Course on the Ethical, Legal, and Social Issues of Biobanking Practice-A Participatory Action Research Study in South Africa.","authors":"Shenuka Singh","doi":"10.1089/bio.2023.0165","DOIUrl":"10.1089/bio.2023.0165","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> There is a paucity of available training opportunities on the ethical, legal, and social issues (ELSI) of biobanking in South Africa and other low- and middle-income countries. For this purpose, an online short course was developed on the ELSI of biobanking practice. <b><i>Study Aims and Objectives:</i></b> This study aimed to review the short course to determine its relevance for identified stakeholders in biobanking practice in South Africa. <b><i>Methods:</i></b> This in-depth exploratory study was conducted using a qualitative approach. Two groups of volunteers were purposively identified for the review of the course. Group 1 (Biobanking group, <i>n</i> = 11) comprised researchers, biobankers, postgraduate students in biobanking research, and research ethics committee members. Group 2 (Curriculum group, <i>n</i> = 10) comprised academics with expertise in curriculum development and review who were invited to participate in the study. A separate online open-ended questionnaire was used to collect data from each group. Both questionnaires focused on the description of the module structure and coherence. In addition, participants in Group 2 were asked to comment on the assessment strategy used. Thematic analysis was conducted on the collected data. <b><i>Summary of the Study Findings:</i></b> The following themes were identified as strengths and shortcomings of the developed course and suggestions to improve both the content and delivery of the course. Participants were generally satisfied with the course design and structure. The module content was seen as being clear and aligned with the learning objectives. While the course structure was seen as easy to follow, some respondents did express difficulty in navigating through the modules while others experienced varying online technical problems. The general opinion was that the assessment strategy was consistent with the course aim and objectives. <b><i>Conclusion:</i></b> Study participants responded positively to this course and provided constructive criticism to improve the educational offering.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"563-567"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141560428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biopreservation and Biobanking-A Look Back at 2024. 生物保存和生物库--回望 2024 年。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.1089/bio.2024.0152
Marianna J Bledsoe
{"title":"<i>Biopreservation and Biobanking</i>-A Look Back at 2024.","authors":"Marianna J Bledsoe","doi":"10.1089/bio.2024.0152","DOIUrl":"10.1089/bio.2024.0152","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"541-542"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stability and Qualification of a Legacy Fungal Collection. 遗留真菌藏品的稳定性和鉴定。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-01 Epub Date: 2024-04-30 DOI: 10.1089/bio.2023.0154
Mennat El Ghalid, Adriana Chiarelli, Sylvain Brisse, Fay Betsou, Dea Garcia-Hermoso
{"title":"Stability and Qualification of a Legacy Fungal Collection.","authors":"Mennat El Ghalid, Adriana Chiarelli, Sylvain Brisse, Fay Betsou, Dea Garcia-Hermoso","doi":"10.1089/bio.2023.0154","DOIUrl":"10.1089/bio.2023.0154","url":null,"abstract":"<p><p><b><i>Background:</i></b> Microbial culture collections are valuable repositories for qualified and diverse microorganisms, playing a pivotal role in research, education, innovation, as well as in our response to current and emerging public health and societal challenges. However, such precious holdings, when not integrated in professional biobank infrastructures, may be vulnerable to major risks such as staff retirement, changes in the institutional strategy, or natural disasters. The process of preserving and rescuing \"historical\" collections can be long and treacherous with a loss of a part of the collection. At the Biological Resource Center of Institut Pasteur, we undertook the challenge of rescuing the dormant legacy fungal collection. <b><i>Materials and Methods</i>:</b> A total of 64 freeze-dried strains, including yeasts and filamentous fungi, were characterized by using a polyphasic approach combining morphological features and molecular data. We assessed the viability, purity, and authenticity of selected strains isolated from multiple sources and stored for more than 20 years. <b><i>Results:</i></b> Our preliminary results show long-term stability of the selected strains and successful qualification in terms of purity and authentication. Moreover, based on the most recent taxonomic revisions, we updated and revised the nomenclature, where applicable. <b><i>Conclusion:</i></b> Our findings demonstrated the potential value of reviving historical microbial collections for biobanking and research activities and reassure us about the collection's future reopening.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"586-596"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Apoptotic-Related MiRNAs Correlated with Functional and Flow Cytometric Parameters in Asthenozoospermic Holstein Bulls After Freeze-Thaw Process. 与凋亡相关的 MiRNA 与冻融过程后无精子荷斯坦公牛的功能和流式细胞仪参数的关系
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-01 Epub Date: 2024-04-30 DOI: 10.1089/bio.2023.0135
Morteza Taravat, Amirmahdi Roshanzamir, Maryam Rahbar, Tohid Rezaei Topraggaleh, Reza Asadpour, Mustafa Numan Bucak
{"title":"Apoptotic-Related MiRNAs Correlated with Functional and Flow Cytometric Parameters in Asthenozoospermic Holstein Bulls After Freeze-Thaw Process.","authors":"Morteza Taravat, Amirmahdi Roshanzamir, Maryam Rahbar, Tohid Rezaei Topraggaleh, Reza Asadpour, Mustafa Numan Bucak","doi":"10.1089/bio.2023.0135","DOIUrl":"10.1089/bio.2023.0135","url":null,"abstract":"<p><p>Many cellular processes in spermatozoa, including apoptosis and motility, are regulated by miRNA. Different miRNAs and molecular pathways are involved in asthenozoospermia (AS) conditions, which are thought to be one of the causes of infertility with reduced sperm motility. Thirty-two semen samples from four Holstein bulls with normozoospermia (NS), total motility ≥ 70%, and progressive motility ≥ 60%, and 32 semen samples from four bulls with AS, total motility ≤ 40%, and progressive motility ≤ 32% were used to investigate the function of apoptosis-related miRNAs in the AS group. Samples were then aspirated into a 0.5 mL straw after dilution with a Tris-egg yolk extender and frozen at -196°C. After freezing, semen samples were thawed for 2 weeks at 37°C and sperm kinematic parameters, plasma membrane integrity, acrosome integrity, DNA fragmentation, apoptosis status, and expression of apoptosis-related miRNAs (miR-2114, miR-296-3p, miR-455-3p, and miR345-3p) were evaluated. Our results showed that the functional and flow cytometric parameters of the NS group were significantly better than those of the AS group. In the NS group, miR-455-3pp and miR-2412 were upregulated, while miR-345-3p was downregulated compared with the AS group. In the AS group, miR-296-39, miR-2412, and miR-345-3p levels were strongly correlated with membrane integrity, DNA fragmentation, and apoptosis status. The findings demonstrated that the selected miRNAs based on bioinformatic analysis in AS and NS samples had a substantial association with functional and flow cytometry indicators and may be involved in regulating apoptosis and motility in AS samples.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"616-627"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140869272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Applying Findable, Accessible, Interoperable, and Reusable Principles to Biospecimens and Biobanks. 将可查找、可访问、可互操作和可重复使用原则应用于生物样本和生物库。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-01 Epub Date: 2024-02-12 DOI: 10.1089/bio.2023.0110
Amanda Rush, Jennifer A Byrne, Peter H Watson
{"title":"Applying Findable, Accessible, Interoperable, and Reusable Principles to Biospecimens and Biobanks.","authors":"Amanda Rush, Jennifer A Byrne, Peter H Watson","doi":"10.1089/bio.2023.0110","DOIUrl":"10.1089/bio.2023.0110","url":null,"abstract":"<p><p>The importance of stimulating greater sharing of data for use and reuse in health research is widely recognized. To this end, the findable, accessible, interoperable, and reusable (FAIR) principles for data have been developed and widely accepted in the research community. Research biospecimens are a resource that leads to much of this health research data but are also a form of data. Therefore, the FAIR principles should apply to biospecimens. Nevertheless, there is a widespread problem of not sharing biospecimen resources that is clearly visible within the research arena. The impacts of this are likely to include diversion of precious research funds into compiling duplicate biospecimen cohorts, detraction from research productivity as researchers compete for and create duplicate resources, and deterrence of attempts to assess research reproducibility. This article explores some of the barriers that may limit availability of FAIR biospecimens. These barriers relate to the type of biospecimen collections and the characteristics of the custodians that influence their intention and interest in sharing. Barriers also relate to the ethical, legal, and social issues concerning collections, the research context of the collections, and cost and expertise involved in repurposing collections to enable sharing. Several solutions to increase sharing are identified. Some have recently been implemented, including enhancing biospecimen locators with tools to guide researchers and facilitating transfer of research collections to centralized biobank infrastructures at the conclusion of projects. New proposed solutions include improving search capabilities within publication databases, and introduction of evidence-based justifications for all new collections into peer-reviewed grant competition processes. It is recognized that there are both scientific factors and practical reasons that can impose limits to sharing biospecimens. However, funding availability, productivity, and progress in health research all stand to benefit from improved sharing of research biospecimen collections.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"550-556"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139725037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experts Speak Forum: Implementation of the FAIR Principles in Biobanking Needs Fair Incentives. 专家演讲论坛:生物银行公平原则的实施需要公平的激励。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-01 Epub Date: 2024-12-05 DOI: 10.1089/bio.2024.0153
Daniel Simeon-Dubach, Zisis Kozlakidis, Juhi Tayal, Shannon J McCall, Wohaib Hasan, Fay Betsou, Jonathan Lawson, Dominic Allen
{"title":"Experts Speak Forum: Implementation of the FAIR Principles in Biobanking Needs Fair Incentives.","authors":"Daniel Simeon-Dubach, Zisis Kozlakidis, Juhi Tayal, Shannon J McCall, Wohaib Hasan, Fay Betsou, Jonathan Lawson, Dominic Allen","doi":"10.1089/bio.2024.0153","DOIUrl":"10.1089/bio.2024.0153","url":null,"abstract":"<p><p>While the FAIR (Findable, Accessible, Interoperable, and Reusable) principles are primarily concerned with data, samples can also be considered a distinct category of data. In light of these considerations, the FAIR principles represent a major challenge for biobanks, as discussed in detail in two recently published studies. We invited seven experts with diverse backgrounds to share their views on these studies and the FAIR principles in general. The contributions are written from different perspectives, including those from human biobanks operating globally, located in low- or middle-income countries or in high-income countries, as well as those from industrial or environmental biobanks. The last two contributions focused on technical feasibility and the necessary incentives. All authors agreed that while the FAIR principles present a challenge for biobanks, they also offer opportunities. Various useful instruments already exist, and more will follow. The key is to provide meaningful incentives.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"557-562"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Technology for Biobanking of Epididymal Spermatozoa from Patient with Obstructive Azoospermia: Case Report about Baby Born after Conventional Freezing Only with a Nonpermeable Cryoprotectant 360 kDa Polyvinylpyrrolidone. 阻塞性无精子症患者附睾精子的生物库技术:仅使用非渗透性冷冻保护剂 360 kDa 聚乙烯吡咯烷酮进行传统冷冻后出生婴儿的病例报告。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-12-01 Epub Date: 2024-03-18 DOI: 10.1089/bio.2023.0032
Maryna Petrushko, Taisiia Yurchuk, Volodymyr Piniaiev, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Volodimir Isachenko
{"title":"Technology for Biobanking of Epididymal Spermatozoa from Patient with Obstructive Azoospermia: Case Report about Baby Born after Conventional Freezing Only with a Nonpermeable Cryoprotectant 360 kDa Polyvinylpyrrolidone.","authors":"Maryna Petrushko, Taisiia Yurchuk, Volodymyr Piniaiev, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Volodimir Isachenko","doi":"10.1089/bio.2023.0032","DOIUrl":"10.1089/bio.2023.0032","url":null,"abstract":"<p><p>This publication reports, for the first time, the birth of a healthy child after intracytoplasmic sperm injection (ICSI) of motile spermatozoa after conventional (\"slow\") freezing of epididymal spermatozoa using 5% polyvinylpyrrolidone (PVP) of high molecular weight (360 kDa). Cryopreservation solution with 10% PVP was added to 30 µL of spermatozoa suspension in a 1:1 ratio, with a final PVP concentration of 5%. Then, polycarbonate capillaries for oocyte denudation with a diameter of 170 µm were filled with 60 µL of the resulting sperm suspension. After that, the capillaries were placed for 10 minutes at a height of 15 cm above liquid nitrogen and immersed into liquid nitrogen. To warm the spermatozoa, the capillaries were immersed in a water bath at a temperature of 40°C for 30 seconds. Oocyte fertilization was performed by ICSI. Zygotes were cultured <i>in vitro</i> for 5 days to the blastocyst stage. More than 100 spermatozoa were obtained after percutaneous epidydimal sperm aspiration, of which 80% were motile. After cryopreservation, storage for 3 months in liquid nitrogen, and thawing, 72% of the total sperm cells remained motile. Ten oocyte-cumulus complexes were found after follicle puncture, and eight metaphase II stage oocytes were fertilized using ICSI. After 18 hours, two pronuclei were found in seven (88%) of the oocytes. An analysis of the morphological characteristics of 5-day-old embryos showed that four (57%) of them reached the blastocyst stage. One embryo was transferred, and the remaining embryos were cryopreserved (vitrified). The onset of pregnancy was detected on the 14th day after embryo transfer, and one healthy girl (3300 g) was born at term.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"597-599"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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