Biopreservation and Biobanking最新文献

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Analysis of the Nonequilibrium Phase Change Behaviors of the Cryoprotectant Solutions for Cryopreservation of Human Red Blood Cells with Low-Concentration Glycerol. 用低浓度甘油冷冻保存人类红细胞的低温保护剂溶液的非平衡相变行为分析。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-08-01 Epub Date: 2024-01-09 DOI: 10.1089/bio.2023.0041
Xingjie Wu, Lingxiao Shen, Gang Zhao
{"title":"Analysis of the Nonequilibrium Phase Change Behaviors of the Cryoprotectant Solutions for Cryopreservation of Human Red Blood Cells with Low-Concentration Glycerol.","authors":"Xingjie Wu, Lingxiao Shen, Gang Zhao","doi":"10.1089/bio.2023.0041","DOIUrl":"10.1089/bio.2023.0041","url":null,"abstract":"<p><p>Recently, we proposed a low-glycerol cryoprotectant formulation (consisting of 0.4 M trehalose and 5% glycerol) for cryopreservation of human red blood cells (RBCs), which greatly reduced the concentration of glycerol, minimized intracellular ice damage, and achieved high recovery. Although this study was successful in cellular experiments, the nonequilibrium phase transition behaviors of the cryoprotective agent solution have not been systematically analyzed. Therefore, it is essential to provide reliable thermodynamic data to substantiate the viability of this cryopreservation technique. In this study, the phase change behaviors and thermal properties of typical trehalose and/or glycerol solutions quenched in liquid nitrogen were investigated using differential scanning calorimetry and cryomicroscopy. It was found that the glass transition temperatures of both the trehalose aqueous solution (<1.0 M) and glycerol aqueous solution (<40% w/v) did not vary apparently with the concentration at low concentrations, while they increased significantly with increasing concentration at high concentrations. Moreover, it was revealed that the inhibitory effect of trehalose on ice growth was affected by glycerol. We further found that the addition of low concentrations of glycerol facilitates the partial glass transition of trehalose solutions at low concentrations. The results of this work provide reliable thermodynamic data to support the cryopreservation of human RBCs with unusually low concentrations of glycerol.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139405399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research Use of Biospecimens from Deceased Individuals: A Call for Greater Regulatory Harmonization. 研究使用已故个人的生物样本:呼吁加强监管协调。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-08-01 DOI: 10.1089/bio.2024.0107
Marianna J Bledsoe, Rita T Lawlor, Helen R Morrin
{"title":"Research Use of Biospecimens from Deceased Individuals: A Call for Greater Regulatory Harmonization.","authors":"Marianna J Bledsoe, Rita T Lawlor, Helen R Morrin","doi":"10.1089/bio.2024.0107","DOIUrl":"https://doi.org/10.1089/bio.2024.0107","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Memory of Virginia A. LiVolsi, MD. 纪念医学博士 Virginia A. LiVolsi。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-08-01 Epub Date: 2024-08-05 DOI: 10.1089/bio.2024.0100
Diane McGarvey
{"title":"In Memory of Virginia A. LiVolsi, MD.","authors":"Diane McGarvey","doi":"10.1089/bio.2024.0100","DOIUrl":"10.1089/bio.2024.0100","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing Decisional Regret Among Biobank Sample Donors. 评估生物库样本捐献者的后悔决策。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-07-29 DOI: 10.1089/bio.2024.0053
Sara Watson, Joel E Pacyna, Janet E Olson, Richard R Sharp
{"title":"Assessing Decisional Regret Among Biobank Sample Donors.","authors":"Sara Watson, Joel E Pacyna, Janet E Olson, Richard R Sharp","doi":"10.1089/bio.2024.0053","DOIUrl":"https://doi.org/10.1089/bio.2024.0053","url":null,"abstract":"<p><p><b><i>Background:</i></b> Large biobanks that link biological specimens with specimen donors' health histories are a critical tool for precision medicine, and many health care institutions have invested significant resources in setting up and building up large collections for this purpose. As biobanks require consented participation from thousands of individual donors, much research has focused on the values and preferences of new and prospective donors who are actively contemplating an invitation to participate in the collection. Few studies, however, have focused on participants' opinions about their biobank participation in the months and years following enrollment. <b><i>Methods:</i></b> We conducted a survey in a large, established biobank and evaluated participants' levels of decisional regret regarding their decision to enroll in the biobank. <b><i>Results:</i></b> We found very low levels of decisional regret among established biobank participants. Multivariable regression analysis found that age, length of time in the biobank, lower educational attainment, inadequate health literacy, and previous invitations to research participation were all significant predictors of elevated regret. <b><i>Discussion:</i></b> Among those with elevated regret, several demographic factors may point to elevated likelihood of decisional regret. More research is needed to identify factors associated with long-term satisfaction with biobank participation and with elevated risk of regret and/or withdrawal from the collection.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Malian-German Network: Opportunities and Challenges in the Development of Academic Biobanks and Comparative Biomedical Research. 马里-德国网络:发展学术生物库和比较生物医学研究的机遇与挑战。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-07-10 DOI: 10.1089/bio.2024.0038
Saidou Balam, Lina Winter, Annalena Artinger, Haby Konaté, Fousseyni Diarra, Mamoudou Maiga, Edward K Geissler, Bakrou Kamaté, Cheick B Traoré, Christoph Brochhausen
{"title":"The Malian-German Network: Opportunities and Challenges in the Development of Academic Biobanks and Comparative Biomedical Research.","authors":"Saidou Balam, Lina Winter, Annalena Artinger, Haby Konaté, Fousseyni Diarra, Mamoudou Maiga, Edward K Geissler, Bakrou Kamaté, Cheick B Traoré, Christoph Brochhausen","doi":"10.1089/bio.2024.0038","DOIUrl":"https://doi.org/10.1089/bio.2024.0038","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
L-Proline as a Cryoprotective Agent for the Preservation of Galea Spixii Skin Fibroblasts. L-脯氨酸作为一种低温保护剂用于保存 Galea Spixii 皮肤成纤维细胞
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-07-10 DOI: 10.1089/bio.2024.0006
Leonardo V C Aquino, Luanna L V Rodrigues, Samara L Olindo, Yara L F Silva, Lhara R M Oliveira, Yasmin B F Moura, Alexsandra F Pereira
{"title":"L-Proline as a Cryoprotective Agent for the Preservation of <i>Galea Spixii</i> Skin Fibroblasts.","authors":"Leonardo V C Aquino, Luanna L V Rodrigues, Samara L Olindo, Yara L F Silva, Lhara R M Oliveira, Yasmin B F Moura, Alexsandra F Pereira","doi":"10.1089/bio.2024.0006","DOIUrl":"https://doi.org/10.1089/bio.2024.0006","url":null,"abstract":"<p><p>Somatic cell biobanking is a promising strategy for developing reproductive techniques. Although cryopreservation, a technique used for creating biobanks, has been performed on <i>Galea spixii</i>, structural and physiological damage to its cells highlight the need to optimize the cryoprotective solution being used. Therefore, the osmoprotective activity of 5 mM L-proline was evaluated as an alternative cryoprotectant for <i>G. spixii</i> fibroblast conservation. The concentration was defined based on previous studies conducted on mammalian cells. Cells derived from the skin of six individuals were cultured until the fifth passage were cryopreserved under the following treatments: (i) control (non-cryopreserved); (ii) a solution with 10% dimethyl sulfoxide (Me<sub>2</sub>SO), 10% fetal bovine serum (FBS), and 0.2 M sucrose; (iii) a solution with 10% Me<sub>2</sub>SO, 10% FBS, and 5 mM L-proline; and (iv) a solution with 10% Me<sub>2</sub>SO, 10% FBS, 0.2 M sucrose, and 5 mM L-proline. Tests were conducted to analyze cell morphology, viability, metabolism, proliferation, and apoptosis; reactive oxygen species (ROS) levels; and mitochondrial membrane activity (ΔΨm). A reduction in the number of viable cells (72.3% ± 1.2%) was observed in the sucrose-containing group compared to the control (86.7% ± 2.0%) and L-proline (88.4% ± 1.8% and 87.8% ± 2.1%) groups. After apoptotic analysis, a reduction in the number of viable cells was observed in the group with sucrose alone (74.6% ± 4.1%) compared to the control group (88.2% ± 1.1%). The ROS levels (1.03 ± 0.5 and 1.07 ± 0.5, respectively) and ΔΨm values (0.99 ± 0.42 and 1.22 ± 0.73, respectively) observed in the groups with L-proline were similar to that observed in the control group (1.00 ± 0.5 and 1.00 ± 0.4, respectively). Moreover, no difference was observed between groups for cell morphology, metabolism, or proliferation. Thus, L-proline is a cryoprotectant agent that can be used during <i>G. spixii</i> fibroblast cryopreservation, alone or with sucrose. In addition, we developed an adequate biobank for <i>G. spixii</i>, whereby stored cells could be used for reproductive techniques.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Developing a Short Course on the Ethical, Legal, and Social Issues of Biobanking Practice-A Participatory Action Research Study in South Africa. 在南非开发关于生物银行实践中的伦理、法律和社会问题的短期课程--参与式行动研究。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-07-08 DOI: 10.1089/bio.2023.0165
Shenuka Singh
{"title":"Developing a Short Course on the Ethical, Legal, and Social Issues of Biobanking Practice-A Participatory Action Research Study in South Africa.","authors":"Shenuka Singh","doi":"10.1089/bio.2023.0165","DOIUrl":"https://doi.org/10.1089/bio.2023.0165","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> There is a paucity of available training opportunities on the ethical, legal, and social issues (ELSI) of biobanking in South Africa and other low- and middle-income countries. For this purpose, an online short course was developed on the ELSI of biobanking practice. <b><i>Study Aims and Objectives:</i></b> This study aimed to review the short course to determine its relevance for identified stakeholders in biobanking practice in South Africa. <b><i>Methods:</i></b> This in-depth exploratory study was conducted using a qualitative approach. Two groups of volunteers were purposively identified for the review of the course. Group 1 (Biobanking group, <i>n</i> = 11) comprised researchers, biobankers, postgraduate students in biobanking research, and research ethics committee members. Group 2 (Curriculum group, <i>n</i> = 10) comprised academics with expertise in curriculum development and review who were invited to participate in the study. A separate online open-ended questionnaire was used to collect data from each group. Both questionnaires focused on the description of the module structure and coherence. In addition, participants in Group 2 were asked to comment on the assessment strategy used. Thematic analysis was conducted on the collected data. <b><i>Summary of the Study Findings:</i></b> The following themes were identified as strengths and shortcomings of the developed course and suggestions to improve both the content and delivery of the course. Participants were generally satisfied with the course design and structure. The module content was seen as being clear and aligned with the learning objectives. While the course structure was seen as easy to follow, some respondents did express difficulty in navigating through the modules while others experienced varying online technical problems. The general opinion was that the assessment strategy was consistent with the course aim and objectives. <b><i>Conclusion:</i></b> Study participants responded positively to this course and provided constructive criticism to improve the educational offering.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141560428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association of Serum Metabolites with Serum Indices and Preanalytical Factors of Biobanked Serum Samples. 血清代谢物与生物库血清样本的血清指标和分析前因素的关系
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-07-08 DOI: 10.1089/bio.2023.0130
Juyoung Lee, Byeong-Gon Ji, Eun-Jung Hong, Jae-Pil Jeon
{"title":"Association of Serum Metabolites with Serum Indices and Preanalytical Factors of Biobanked Serum Samples.","authors":"Juyoung Lee, Byeong-Gon Ji, Eun-Jung Hong, Jae-Pil Jeon","doi":"10.1089/bio.2023.0130","DOIUrl":"https://doi.org/10.1089/bio.2023.0130","url":null,"abstract":"<p><p><b><i>Background:</i></b> Serum indices (hemolysis, icterus, and lipemia; HIL) are known to impact clinical chemistry assay results. This study aimed to investigate the impact of HIL indices on serum metabolite profiles and the association of serum metabolite levels with pre-analytical factors of serum samples. <b><i>Methods:</i></b> A cohort of serum samples (<i>n</i> = 12,196) from the Korean Genome and Epidemiology Study (KoGES) was analyzed for HIL indices and the pre-analytical variables (SPRECs) which were generated in the process of serum collection. We further performed targeted metabolomics on a subset comprising hemolyzed (<i>n</i> = 60), icteric (<i>n</i> = 60), lipemic (<i>n</i> = 60) groups, and a common control group of non-HIL samples (<i>n</i> = 60) using the Absolute IDQ p180 kit. <b><i>Results:</i></b> We found 22 clinical chemistry analytes significantly associated with hemolysis, 25 with icterus, and 24 with lipemia (<i>p</i> < 0.0001). Serum metabolites (<i>n</i> = 27) were associated with all of hemolysis, icterus, and lipemia (<i>p</i> < 0.05). The PC ae C36 2 had exhibited a significant association with pre-analytical factors corresponding to the third (pre-centrifugation delay between processing) and sixth (post-centrifugation) elements of the SPREC. <b><i>Conclusions:</i></b> This study showed the association of the serum index and pre-analytical factors with serum metabolite profiles. In addition, the association of pre-analytical factors with serum metabolite concentrations would corroborate the utility of SPRECs for the quality control of biobanked serum samples.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Technical Guideline for Low-Temperature Preservation of Pathogenic Microorganisms in China and Its Relevance to Developing Countries. 中国病原微生物低温保存技术指南及其对发展中国家的意义》。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-07-04 DOI: 10.1089/bio.2024.0022
Io Hong Cheong, Yuanyuan Zhao, Mengnan Jiang, Suwen Lei, Hui Wang, Zisis Kozlakidis, Qiang Wei
{"title":"Technical Guideline for Low-Temperature Preservation of Pathogenic Microorganisms in China and Its Relevance to Developing Countries.","authors":"Io Hong Cheong, Yuanyuan Zhao, Mengnan Jiang, Suwen Lei, Hui Wang, Zisis Kozlakidis, Qiang Wei","doi":"10.1089/bio.2024.0022","DOIUrl":"10.1089/bio.2024.0022","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Role of Skin-Derived Somatic Cell and Tissue Cryobanks in the Conservation of Aquatic Mammals. 源自皮肤的体细胞和组织冷冻库在水生哺乳动物保护中的作用。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2024-07-03 DOI: 10.1089/bio.2023.0168
Luanna Lorenna Vieira Rodrigues, Radan Elvis Matias de Oliveira, Fernanda Löffler Niemeyer Attademo, Alexsandra Fernandes Pereira
{"title":"The Role of Skin-Derived Somatic Cell and Tissue Cryobanks in the Conservation of Aquatic Mammals.","authors":"Luanna Lorenna Vieira Rodrigues, Radan Elvis Matias de Oliveira, Fernanda Löffler Niemeyer Attademo, Alexsandra Fernandes Pereira","doi":"10.1089/bio.2023.0168","DOIUrl":"https://doi.org/10.1089/bio.2023.0168","url":null,"abstract":"<p><p>Anthropogenic actions, especially inadequate waste disposal, cause permanent effects on aquatic fauna, resulting in a significant loss in their population. In this scenario, <i>in situ</i> and <i>ex situ</i> conservation strategies have been developed for these species. Among these strategies is the formation of somatic cell and tissue banks derived from skin collection that act complementarily to other biotechnologies. These banks contain all the information for genomic, genetic, and proteomic analyses. They are useful in the assessment of the toxicity of pollutants on the physiology of the species and regenerative and reproductive biotechnologies. The formation of these cryobanks involves different steps, including cryopreservation, with the optimization of all steps occurring in a species-specific manner. There is a diversity of studies on aquatic mammals; however, a low quantity compared to the number of studies on land mammals, with more than 80% of species still unexplored. This is mainly due to the difficulty of execution and asepsis in collecting skin from aquatic mammals and the <i>in vitro</i> culture, which seems to require more particularities for it to be successful. Therefore, this review aims to address the current scenario and the steps involved in the conservation of somatic cells and tissues derived from aquatic mammal skin, as well as results that have been achieved in recent years and the prospects.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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