Biopreservation and Biobanking最新文献_第10页

Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields. 交流电场作用下二甲亚砜溶液冻结过程中冰晶生长的定量分析。

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-08-01 Epub Date: 2023-11-22 DOI: 10.1089/bio.2023.0035

Liting Liu, Zirui Wang, Menghan Wang, Gang Zhao

{"title":"Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields.","authors":"Liting Liu, Zirui Wang, Menghan Wang, Gang Zhao","doi":"10.1089/bio.2023.0035","DOIUrl":"10.1089/bio.2023.0035","url":null,"abstract":"During cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a strongly polar dielectric material, and the applied alternating current (AC) electric field will affect the water freezing performance. Therefore, a quantitative study of ice crystal nucleation and growth during freezing of dimethyl sulfoxide solutions under different AC electric field conditions is needed to try to reduce ice crystal damage. We created a liquid-film device to approximate the ice crystal growth process as a two-dimensional image. The frequency of the AC voltage was set from 0 to 50 kHz. We measured the supercooling of the dimethyl sulfoxide solution under AC electric field conditions. As an objective and accurate quantitative analysis of the ice crystal growth process, we propose a Dilated Convolutional Segmentation Transformer for semantic segmentation of ice crystal images. It is concluded that the average area and the growth rate of single ice crystals decrease with increasing electric field frequency at a certain concentration of dimethyl sulfoxide solution. Lower concentrations of dimethyl sulfoxide solution in combination with an AC electric field can achieve similar ice suppression effects as when higher concentrations of dimethyl sulfoxide solution act alone. We believe that AC electric fields are expected to be an aid to cryopreservation and provide some theoretical basis and experimental foundation for its development.","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"383-394"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138447154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Use of Biospecimens from Deceased Individuals: A Call for Greater Regulatory Harmonization. 研究使用已故个人的生物样本：呼吁加强监管协调。

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-08-01 DOI: 10.1089/bio.2024.0107

Marianna J Bledsoe, Rita T Lawlor, Helen R Morrin

引用次数: 0

Analysis of the Nonequilibrium Phase Change Behaviors of the Cryoprotectant Solutions for Cryopreservation of Human Red Blood Cells with Low-Concentration Glycerol. 用低浓度甘油冷冻保存人类红细胞的低温保护剂溶液的非平衡相变行为分析。

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-08-01 Epub Date: 2024-01-09 DOI: 10.1089/bio.2023.0041

Xingjie Wu, Lingxiao Shen, Gang Zhao

{"title":"Analysis of the Nonequilibrium Phase Change Behaviors of the Cryoprotectant Solutions for Cryopreservation of Human Red Blood Cells with Low-Concentration Glycerol.","authors":"Xingjie Wu, Lingxiao Shen, Gang Zhao","doi":"10.1089/bio.2023.0041","DOIUrl":"10.1089/bio.2023.0041","url":null,"abstract":"Recently, we proposed a low-glycerol cryoprotectant formulation (consisting of 0.4 M trehalose and 5% glycerol) for cryopreservation of human red blood cells (RBCs), which greatly reduced the concentration of glycerol, minimized intracellular ice damage, and achieved high recovery. Although this study was successful in cellular experiments, the nonequilibrium phase transition behaviors of the cryoprotective agent solution have not been systematically analyzed. Therefore, it is essential to provide reliable thermodynamic data to substantiate the viability of this cryopreservation technique. In this study, the phase change behaviors and thermal properties of typical trehalose and/or glycerol solutions quenched in liquid nitrogen were investigated using differential scanning calorimetry and cryomicroscopy. It was found that the glass transition temperatures of both the trehalose aqueous solution (<1.0 M) and glycerol aqueous solution (<40% w/v) did not vary apparently with the concentration at low concentrations, while they increased significantly with increasing concentration at high concentrations. Moreover, it was revealed that the inhibitory effect of trehalose on ice growth was affected by glycerol. We further found that the addition of low concentrations of glycerol facilitates the partial glass transition of trehalose solutions at low concentrations. The results of this work provide reliable thermodynamic data to support the cryopreservation of human RBCs with unusually low concentrations of glycerol.","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"325-335"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139405399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Memory of Virginia A. LiVolsi, MD. 纪念医学博士 Virginia A. LiVolsi。

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-08-01 Epub Date: 2024-08-05 DOI: 10.1089/bio.2024.0100

Diane McGarvey

引用次数: 0

Biobanking Legislation in Spain: Advancing or Undermining Its Ethical Values? 西班牙的生物银行立法：西班牙的生物银行立法：促进还是削弱其伦理价值？

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-06-01 Epub Date: 2024-01-18 DOI: 10.1089/bio.2023.0107

Inmaculada de Melo-Martín, Eva Ortega-Paíno

引用次数: 0

Dare to Dream-Building the Future of Biobanking: A Summary of the ISBER Annual Meeting, April 9-12, 2024. 敢于梦想--打造生物银行的未来：2024年4月9日至12日ISBER年会摘要。

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-06-01 DOI: 10.1089/bio.2024.0073

Cassandra Griffin, Gregory Grossman, Amanda Moors

引用次数: 0

Increase in Serum Potassium Levels After Refrigerated Storage: A Component of Blood Clot Contaminates the Serum Layer Over the Separator Gel. 冷藏后血清钾水平升高：血凝块的一种成分污染了分离凝胶上的血清层。

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-06-01 Epub Date: 2023-11-09 DOI: 10.1089/bio.2022.0217

Kohsuke Yoshida, Hiromi Tani, Sae Nobuhara, Shinji Yamamoto

{"title":"Increase in Serum Potassium Levels After Refrigerated Storage: A Component of Blood Clot Contaminates the Serum Layer Over the Separator Gel.","authors":"Kohsuke Yoshida, Hiromi Tani, Sae Nobuhara, Shinji Yamamoto","doi":"10.1089/bio.2022.0217","DOIUrl":"10.1089/bio.2022.0217","url":null,"abstract":"Objectives: The aim of this study was to determine the cause of elevated serum potassium levels when blood collection tubes containing separating gel are stored under refrigeration. Methods: Fifty-seven hospitalized patients and 11 healthy volunteers were recruited. Venous blood samples were obtained using Insepac II, Neotube, and Venoject® II, without anticoagulant. After centrifugation under different processing conditions, the capped tubes were stored at 4°C without aliquoting, and serum potassium levels were measured for up to 14 days. Correlation between the increase in potassium levels and blood cell counts was assessed. Furthermore, serum was replaced with a saline solution and potassium levels were determined after refrigeration. Results: Refrigerated samples stored in Insepac II tubes had significantly higher serum potassium levels on day 14 than on the day of blood collection. The increase in serum potassium levels was positively correlated with the number of red blood cells, but not white blood cells and platelets in venous blood. Furthermore, potassium levels were elevated when serum was replaced with a saline solution. Using Venoject II, which has a larger tube diameter and thicker separating gel than those of Insepac II and Neotube, did not increase serum potassium levels after storage. Increase in the serum potassium level was markedly suppressed by centrifugation at 2330 g for 15 minutes relative to other processing conditions. Conclusions: Potassium levels increase when serum is refrigerated in collection tubes containing separating gel. This can be attributed to contamination of the serum layer by blood cell components beyond the separating gel.","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"235-241"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72016148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Ferulic Acid, Tryptophan, and L-Glutamine on the Cryopreservation of Mouse Spermatozoa. 阿魏酸、色氨酸和 L-谷氨酰胺对小鼠精子冷冻保存的影响

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-06-01 Epub Date: 2023-12-26 DOI: 10.1089/bio.2023.0067

Gökhan Koçak, Cengiz Yildiz

{"title":"The Effects of Ferulic Acid, Tryptophan, and L-Glutamine on the Cryopreservation of Mouse Spermatozoa.","authors":"Gökhan Koçak, Cengiz Yildiz","doi":"10.1089/bio.2023.0067","DOIUrl":"10.1089/bio.2023.0067","url":null,"abstract":"In this study, the effects of ferulic acid (0.1, 1, ve 10 mM), tryptophan (5, 25, ve 50 mM), and L-glutamine (10, 50, ve 100 mM) at different doses added to 18% raffinose + 3% skimmed milk powder sperm extender on the freezing of mouse spermatozoa in liquid nitrogen were investigated. The combination of 18% raffinose + 3% skimmed milk powder without additives was used as the control group. Frozen spermatozoa were thawed in a 37°C water bath for 30 seconds. After freeze-thawing, motility, dead spermatozoa ratio, plasma membrane integrity, abnormal acrosome ratio, motility endurance (for 4 hours), and cell apoptosis tests were performed in Human Tubal Fluid (HTF). Compared with the control group after freezing and thawing, the highest motility and plasma membrane integrity were obtained in the 10 mM L-glutamine group with 56.6% ± 2.11% and 77.8% ± 0.87%, respectively (p < 0.05). In addition, when compared to the control group, the lowest rate of dead spermatozoa and abnormal acrosome was found in the 10 mM L-glutamine group as 26.0% ± 1.46% and 6.3% ± 1.09%, respectively (p < 0.05). The highest motility values for spermatozoa endurance were determined in the 10 and 50 mM L-glutamine groups up to the 4th hour compared to the control group (p < 0.05). In the evaluation of apoptosis in semen samples, there was no significant difference between the control, 0.1 mM ferulic acid, and 10 mM L-glutamine groups (p > 0.05). As a result, it was determined that the addition of 10 mM L-glutamine to the spermatozoa extender increased the motility, viable spermatozoa, functional membrane integrity, intact acrosome ratios, or motility endurance after freeze-thawing and could be used successfully in the freezing extender of mouse spermatozoa.","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"286-293"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139049748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Call for Papers: Biobanks and Biorepositories in Latin America: Challenges and Opportunities. 征集论文：拉丁美洲的生物库和生物信息库：挑战与机遇。

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-06-01 DOI: 10.1089/bio.2024.47934.cfp

Antonio Hugo J F M Campos, Maria Teresa G de Dávila

引用次数: 0

Biospecimen Qualification in a Clinical Biobank of Urological Diseases. 泌尿系统疾病临床生物库的生物标本鉴定。

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-06-01 Epub Date: 2023-10-25 DOI: 10.1089/bio.2022.0190

Olga Kofanova, Sangita Paul, Achilleas Pexaras, Camille Bellora, Ala Petersons, Margaux Schmitt, Mohamad Baker Berjaoui, Yazan Qaoud, Miran Kenk, Heidi Wagner, Neil Fleshner, Fay Betsou

{"title":"Biospecimen Qualification in a Clinical Biobank of Urological Diseases.","authors":"Olga Kofanova, Sangita Paul, Achilleas Pexaras, Camille Bellora, Ala Petersons, Margaux Schmitt, Mohamad Baker Berjaoui, Yazan Qaoud, Miran Kenk, Heidi Wagner, Neil Fleshner, Fay Betsou","doi":"10.1089/bio.2022.0190","DOIUrl":"10.1089/bio.2022.0190","url":null,"abstract":"Development of novel biomarkers for diagnosis of disease and assessment of treatment efficacy utilizes a wide range of biospecimens for discovery research. The fitness of biospecimens for the purpose of biomarker development depends on the clinical characteristics of the donor and on a number of critical and potentially uncontrolled pre-analytical variables. Pre-analytical factors influence the reliability of the biomarkers to be analyzed and can seriously impact analytic outcomes. Sample quality stratification assays and tools can be utilized by biorepositories to minimize bias resulting from samples' inconsistent quality. In this study, we evaluated the quality of biobanked specimens by comparing analytical outcomes at 1, 5, and 10 years after collection. Our results demonstrate that currently available assays and tools can be used by biobank laboratories to support objective biospecimen qualification. We have established a workflow to monitor the quality of different types of biospecimens and, in this study, present the results of a qualification exercise applied to fluid samples and their derivatives in the context of urological diseases.","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"257-267"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50159394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0