Visualization of Ice Crystal Behavior in Mouse Oocytes During High-Speed Quench Cooling and Ice Inhibition by Antifreezing Hydrogels.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-08-01 Epub Date: 2024-03-14 DOI:10.1089/bio.2023.0108

Xin Li, Shuyong Zhang, Yuqi Zhang, Xinli Zhou

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引用次数: 0

Abstract

Oocyte vitrification has become a widely adopted method in clinical practice. However, the solidification behavior and its impact on oocytes during the ultrarapid cooling process remain poorly understood. In this study, we established a system and methodology to observe crystallization behavior in oocytes during quench cooling and warming. Subsequently, the threshold concentration of cryoprotective agents (CPAs) required for oocyte vitrification was determined through a visualization method. The results demonstrated that the ice front could not be observed in the image sequence when using 16.5% DMSO +16.5% EG during high-speed quench cooling (2821.58°C/min). Finally, oocytes were encapsulated with an antifreezing hydrogel (7.5% EG +7.5% DMSO +0.5% alginate) and subjected to high-speed quench cooling. No ice crystals appeared in the antifreezing hydrogel-encapsulated oocytes at a low concentration of osmotic CPA (2.4 M). This research opens up new possibilities for oocyte vitrification with a reduced concentration of CPA.

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高速骤冷过程中小鼠卵母细胞冰晶行为的可视化以及抗冻水凝胶的冰抑制作用

卵母细胞玻璃化已成为临床上广泛采用的一种方法。然而，人们对超速冷却过程中卵母细胞的凝固行为及其影响仍然知之甚少。在这项研究中，我们建立了一套系统和方法来观察卵母细胞在骤冷和升温过程中的结晶行为。随后，通过可视化方法确定了卵母细胞玻璃化所需的低温保护剂（CPA）阈值浓度。结果表明，在高速骤冷过程中（2821.58°C/分钟）使用 16.5% DMSO +16.5% EG 时，图像序列中无法观察到冰锋。最后，用抗冻水凝胶（7.5% EG +7.5% DMSO +0.5% 藻酸盐）包裹卵母细胞并进行高速骤冷。在低浓度渗透性 CPA（2.4 M）条件下，抗冻水凝胶包裹的卵母细胞中未出现冰晶。这项研究为使用较低浓度的 CPA 进行卵母细胞玻璃化开辟了新的可能性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.