Visualization of Ice Crystal Behavior in Mouse Oocytes During High-Speed Quench Cooling and Ice Inhibition by Antifreezing Hydrogels.

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-08-01 Epub Date: 2024-03-14 DOI:10.1089/bio.2023.0108

Xin Li, Shuyong Zhang, Yuqi Zhang, Xinli Zhou

引用次数: 0

Abstract

Oocyte vitrification has become a widely adopted method in clinical practice. However, the solidification behavior and its impact on oocytes during the ultrarapid cooling process remain poorly understood. In this study, we established a system and methodology to observe crystallization behavior in oocytes during quench cooling and warming. Subsequently, the threshold concentration of cryoprotective agents (CPAs) required for oocyte vitrification was determined through a visualization method. The results demonstrated that the ice front could not be observed in the image sequence when using 16.5% DMSO +16.5% EG during high-speed quench cooling (2821.58°C/min). Finally, oocytes were encapsulated with an antifreezing hydrogel (7.5% EG +7.5% DMSO +0.5% alginate) and subjected to high-speed quench cooling. No ice crystals appeared in the antifreezing hydrogel-encapsulated oocytes at a low concentration of osmotic CPA (2.4 M). This research opens up new possibilities for oocyte vitrification with a reduced concentration of CPA.

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高速骤冷过程中小鼠卵母细胞冰晶行为的可视化以及抗冻水凝胶的冰抑制作用

卵母细胞玻璃化已成为临床上广泛采用的一种方法。然而，人们对超速冷却过程中卵母细胞的凝固行为及其影响仍然知之甚少。在这项研究中，我们建立了一套系统和方法来观察卵母细胞在骤冷和升温过程中的结晶行为。随后，通过可视化方法确定了卵母细胞玻璃化所需的低温保护剂（CPA）阈值浓度。结果表明，在高速骤冷过程中（2821.58°C/分钟）使用 16.5% DMSO +16.5% EG 时，图像序列中无法观察到冰锋。最后，用抗冻水凝胶（7.5% EG +7.5% DMSO +0.5% 藻酸盐）包裹卵母细胞并进行高速骤冷。在低浓度渗透性 CPA（2.4 M）条件下，抗冻水凝胶包裹的卵母细胞中未出现冰晶。这项研究为使用较低浓度的 CPA 进行卵母细胞玻璃化开辟了新的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biopreservation and Biobanking Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology

自引率

12.50%

发文量

114

期刊介绍： Biopreservation and Biobanking is the first journal to provide a unifying forum for the peer-reviewed communication of recent advances in the emerging and evolving field of biospecimen procurement, processing, preservation and banking, distribution, and use. The Journal publishes a range of original articles focusing on current challenges and problems in biopreservation, and advances in methods to address these issues related to the processing of macromolecules, cells, and tissues for research. In a new section dedicated to Emerging Markets and Technologies, the Journal highlights the emergence of new markets and technologies that are either adopting or disrupting the biobank framework as they imprint on society. The solutions presented here are anticipated to help drive innovation within the biobank community. Biopreservation and Biobanking also explores the ethical, legal, and societal considerations surrounding biobanking and biorepository operation. Ideas and practical solutions relevant to improved quality, efficiency, and sustainability of repositories, and relating to their management, operation and oversight are discussed as well.