Biopreservation and Biobanking最新文献

{"title":"Preserving Life on Earth: A Conceptual Model Linking Biobanks, Biotechnology, and Zoological Parks for Biodiversity Hotspots.","authors":"Erik Ruuth, Debbie Romalnis, Lucía Raily Acuña, Tullis Matson, Emanuel M Grassi, Hanne Stabursvik","doi":"10.1177/19475535251384809","DOIUrl":"https://doi.org/10.1177/19475535251384809","url":null,"abstract":"<p><p>The accelerating loss of biodiversity and genetic diversity poses an urgent threat to ecosystems and future generations. This concept article proposes a model centered on biobanks and advanced reproductive biotechnologies as strategic conservation tools, particularly within biodiversity hotspots such as Misiones, Argentina. We outline how institutions like biodiversity institutes, zoos, and biotech firms can collaborate to develop decentralized, scalable cryopreservation capacity. Emphasizing the IMiBio model, we explore how international collaboration, ethical frameworks, and locally grounded governance can support the preservation and future restoration of species. This approach supports both conservation and regeneration, providing a roadmap for hotspot-centered biodiversity recovery.^1-3.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing Ethical Biobanking Through Evolving International Codes: A Call to Action.","authors":"Carol J Weil, Marianna J Bledsoe","doi":"10.1177/19475535251384652","DOIUrl":"https://doi.org/10.1177/19475535251384652","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chilean Biobanks: A Snapshot of the Current Landscape.","authors":"Iskra A Signore, Diego I Romero, Gerardo Donoso, Carolina Selman, Yolanda Espinosa-Parrilla, Macarena Fuentes-Guajardo, Claudia Bambs, Elisa Alcalde, Alejandra Calderón, Camila Corvalán, Sandro Casavilca-Zambrano, Juan Carlos Roa, Alicia Colombo","doi":"10.1177/19475535251379990","DOIUrl":"https://doi.org/10.1177/19475535251379990","url":null,"abstract":"<p><p>Latin America hosts extraordinary biological diversity but remains underrepresented in global biomedical research, underscoring the need for robust biobanking infrastructures. This work provides an updated snapshot of Chilean biobanks, based on a national survey exploring their current capacities and challenges. Nine active biobanks were identified across 5 of Chile's 16 regions, the majority concentrated in Santiago. Collectively, they store over 640,000 biospecimens from nearly 49,000 participants, predominantly oncological. While standardized protocols for sample management are broadly implemented by Chilean biobanks, data management practices are not yet well-developed, as only a few centers have adopted internationally recognized standards. Governance structures vary considerably and often lack formal written documentation. Financial sustainability relies mainly on institutional support, competitive grants, and modest cost recovery. Although Chilean biobanks contribute to research and training, measuring productivity remains challenging due to underreported acknowledgments and limited post-transfer traceability. Overall, our analysis suggests a bottom-up development of Chilean biobanks in the absence of dedicated legislation or strategic governmental policies. This overview shows that Chile's biobanks hold considerable potential for strengthening translational research and health equity, particularly if further support enables expansion into underrepresented regions. By integrating these infrastructures into higher education, clinical care, and broader regional collaborations, biobanks can help leverage Chilean genetic diversity and address health disparities. With greater governmental prioritization, a cohesive regulatory framework, and collaboration as a key strength, biobanks could enhance interaction with global networks and further strengthen Latin America's overall contribution to biomedical innovation.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reporting of Pre-Analytical Processes in Biobanked Biomaterials: A Missed Opportunity to Address the Replicability Crisis in Clinical Biomarker Research.","authors":"Jannes A W Jansen, Mariël A van den Brand, Femmie de Vegt, Dorine W Swinkels","doi":"10.1177/19475535251378202","DOIUrl":"https://doi.org/10.1177/19475535251378202","url":null,"abstract":"<p><p><b><i>Objectives:</i></b> Biobanks are crucial for clinical biomarker research, but pre-analytical processing can impact sample suitability. Insufficient reporting of pre-analytical methods can lead to invalid conclusions. <b><i>Methods:</i></b> This study evaluated the pre-analytical reporting quality in 294 clinical biomarker studies using biobank plasma and serum samples published from 2018 to 2023, based on the Sample PREanalytical Code and the Biospecimen Reporting for Improved Study Quality guidelines. <b><i>Results:</i></b> The analysis revealed frequent incomplete reporting. Key underreported elements included fasting time (reported in 31% of articles), freeze-thaw cycles (22.8%), internal transport (8.5%), and centrifugation settings (20%-35%). Demographic data (96.9%), storage temperatures (81%), and blood tube additives (82.7%) were more consistently reported. No significant correlation was found between reporting quality and journal impact factor. <b><i>Conclusions:</i></b> Our findings highlight the need for standardized pre-analytical reporting to improve research replicability and biomarker study quality.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Low-Density Lipoprotein Supplementation on Post-Thaw Quality and Fertility of Honeybee Semen (<i>Apis mellifera</i> L.).","authors":"Nurdan Coşkun, Aziz Gül, Cengiz Yildiz, Oğuz Kaan Yalçin","doi":"10.1177/19475535251380386","DOIUrl":"https://doi.org/10.1177/19475535251380386","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Long-term storage of bee semen by freezing is a critical process for both the preservation of the genetic material and the sustainability of beekeeping activities. It has been observed that low-density lipoproteins (LDLs) increase sperm quality after freezing and thawing. Although studies have been conducted on the use of LDL for this purpose in different animal species, no research has been conducted on honeybee semen to date. <b><i>Objectives:</i></b> This study aimed to evaluate the potential effects of using LDL instead of egg yolk (EY) on sperm quality and fertilization rate by examining the effects of different LDL ratios (2.5%, 5%, 10%, 25%) on bee semen. <b><i>Methods:</i></b> Sperm collection was conducted using a Schley-type device, resulting in six distinct groups, including both no-supplemented and experimental groups. In the first experiment, sperm collected from 36 drones were diluted with varying LDL concentrations before being frozen and thawed; motility, membrane integrity, viability, and longevity were measured. In the second experiment, a total of 56 virgin sister queens, 8 from each group, were inseminated. <b><i>Results:</i></b> In the group containing 25% LDL, a significant increase was observed in the motility, membrane integrity, and viability rates of frozen-thawed honeybee sperm. In the group containing 25% EY, there was a clear decrease in these parameters; moreover, the lifespan of the sperm was significantly reduced. In the groups, the highest value in terms of fertility was observed in the 25% LDL group, and the lowest value was determined in the 25% EY-added group. <b><i>Conclusion:</i></b> The findings demonstrated that the addition of 25% LDL significantly enhanced both sperm quality and fertility rate in honeybees.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the COVID-19 Pandemic on a Biodiversity Biobank: Response and Adaptation.","authors":"Lucía Raily Acuña, Evelin Yuriko Saguchi, Katherina Alicia Vizcaychipi, Tania Gisel Alarcón, Elias Ivan Delgado, M Emanuel Grassi, Andres Enrique Gustavo Ruuth","doi":"10.1177/19475535251380706","DOIUrl":"https://doi.org/10.1177/19475535251380706","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Biobanks (BBs) are essential for biomedical research and biodiversity conservation. The Misionero Institute of Biodiversity (IMiBio), located in Misiones, Argentina, is dedicated to preserving the Atlantic Forest through a One Health approach, integrating human, animal, and environmental health. During the COVID-19 pandemic, one of its laboratories was adapted for diagnostic testing, leading to the establishment of a landmark repository of viral extracts of global significance. In addition, IMiBio contributed to the detection of SARS-CoV-2 in wildlife, expanding its BB and strengthening epidemiological surveillance efforts. This growth brought significant challenges in standardization and management. This article examines the institute's evolution, achievements, and post-pandemic perspectives. <b><i>Materials and Methods:</i></b> Sample processing is carried out in laboratories corresponding to the specific type of sample received, where they are prepared for entry into the BB. The BB is equipped with -20°C freezers, -80°C ultra-low temperature freezers, and liquid nitrogen tanks to ensure proper preservation of the samples. <b><i>Results:</i></b> The BB of IMiBio initially began by storing samples from wild animals obtained through the Güirá Oga Wildlife Rescue Center (GO). Between 2020 and 2024, the BB integrated over 7,696 samples; 43.98% of BB's storage capacity was utilized. The BB now includes RNA from SARS-CoV-2, arboviruses (dengue and chikungunya), respiratory viruses (influenza, respiratory syncytial virus), DNA from human papillomavirus, and tissue samples and microbial isolates from collaborative research. These additions reinforced BB's role in regional epidemiological surveillance but highlighted challenges in maintaining its original biodiversity focus. <b><i>Conclusions:</i></b> The IMiBio BB has evolved from a biodiversity repository to include biological samples derived from human diagnostics, particularly SARS-CoV-2, thereby strengthening its role in epidemiological surveillance. However, this expansion necessitates balancing its collections to ensure that its original mission of biodiversity conservation is not compromised. A strategic infrastructure expansion is planned for 2025 to enhance capacity, safety, and services.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-Species Variation and Correlation Among Antioxidant Potential, Mitochondrial Performance, and Quality Parameters in Fresh and Cryopreserved Japanese Quail Semen.","authors":"Sumiyyah Zuha, Bushra Allah Rakha, Shamim Akhter","doi":"10.1177/19475535251376471","DOIUrl":"https://doi.org/10.1177/19475535251376471","url":null,"abstract":"<p><p><b><i>Aim:</i></b> The poultry sector is currently witnessing heavy demand for its products especially meat, which is expected to intensify in the coming years. However, while Japanese quail displays a lot of promise to help meet the soaring demands, its sustainable production requires assisted reproduction via sperm cryopreservation. Hence, the current study was designed to elucidate the impact of cryopreservation on Japanese quail semen quality, antioxidant potential, and mitochondrial activity and intra-species variation in terms of freeze-tolerance. <b><i>Materials and Methods:</i></b> Semen was collected individually from seven mature males, diluted with NaCl extender and cryopreserved. Samples were analyzed for sperm motility, plasma membrane and acrosomal integrity, viability, DNA fragmentation, and biochemical parameters at the fresh collection, post-dilution, post-cooling, post-equilibration, and post-thaw stages of freezing. <b><i>Results:</i></b> Sperm motility, plasma membrane and acrosomal integrity, viability, antioxidant potential, scavenging capacity, and mitochondrial activity were reduced (<i>p</i> < 0.05) and DNA fragmentation was increased (<i>p</i> < 0.05) at all the stages of cryopreservation. Further, all the parameters were negatively correlated with DNA fragmentation during cryopreservation. The percent incline rates for DNA fragmentation and decline rates for the rest of the parameters in individual birds showed intra-species variation (<i>p</i> < 0.05) with respect to freeze-tolerance. <b><i>Conclusion:</i></b> Japanese quail semen quality, antioxidant potential, and mitochondrial activity are severely affected by the freezing process and the level of freeze-resilience varies among individuals.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Storage Time up to Nine Years at -25°C on 15 Selected Biochemical Serum Components.","authors":"Steffan Daniel Bos, Marianne Lauritzen, Randi E Gislefoss, Nathalie Støer, Olav I Klingenberg, Hilde Langseth","doi":"10.1177/19475535251371418","DOIUrl":"https://doi.org/10.1177/19475535251371418","url":null,"abstract":"<p><p><b><i>Background:</i></b> Biorepositories facilitate research and clinical studies in many settings. Modern biobanks use state-of-the art storage methods and low temperatures, while many older collections of biospecimens have been stored at less optimal temperatures. The Janus Serum Bank Cohort in Norway holds over 700,000 serum samples collected decades ago and stored at -25°C. To obtain insights in the stability of serum components at -25°C over prolonged times, we performed 7 measurements for increasing storage time up to 108 months for a panel of 15 serum components. <b><i>Method:</i></b> A selection of analytes (proteins, an enzyme, electrolytes, small molecules, hormones, lipids, and a vitamin) were measured in serum from 40 anonymous donors. The serum components were measured in fresh samples and after 3, 6, 12, 24, 36, 72, and 108 months in storage at -25°C. We tested for variations using analysis of variance and paired sample <i>t</i>-tests and performed trend analyses for these serum component levels against time. <b><i>Results:</i></b> All measured serum components showed differences in values for at least one of the timepoints. Trend analyses identified significantly decreasing levels for nine components, whereas four components showed significantly increasing levels. Two components did not show significant trends. <b><i>Conclusion:</i></b> Storage of serum at -25°C may result in changes in serum analyte levels over time. We cannot exclude that batch effects of assaying kits; laboratory instrument changes and standards contributed to the observed differences. To mitigate the influence of increasing storage time, storage time should be used as matching criteria for control samples included in research projects.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Development of the Biobank Collections Valuator as an Automated Tool to Determine the Noneconomic Value of Sample Collections.","authors":"Joseph A Kessler, Tali M Johnson, Marianne K Henderson","doi":"10.1177/19475535251374854","DOIUrl":"https://doi.org/10.1177/19475535251374854","url":null,"abstract":"<p><p>Biobankers rely on their experience, supplemented with a variety of tools, to help establish and sustain their operations. These tools support operations, cost determination, quality management, and governance. Costing tools have often been used to determine the economic value of a single specimen or an entire collection, with the purpose of allowing researchers to recover costs when providing access to those resources. Until recently, biobank managers have focused on deriving sample value based solely on cost-model analyses. We propose an alternative way to value collections using a web-based, automated tool for biobankers to determine the noneconomic value of biospecimen collections. The tool supports fit-for-purpose determinations for collections using common attributes and defined criteria to facilitate broader sample utility, sharing, and overall sustainability in operations.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on Apoptosis of Various Tissues at Different Intervals after Death of Yangtze Sturgeon (<i>Acipenser dabryanus</i>).","authors":"Juanjuan Liu, Binzhong Wang, Yanglingzhi Chen, Shengbo Jiao, Hejun Du","doi":"10.1177/19475535251375775","DOIUrl":"https://doi.org/10.1177/19475535251375775","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> The collection and preservation of postmortem genetic material from recently deceased animals of rare and endangered species represent a critical yet underexplored avenue in conservation biology. While extensive research has been conducted on the human postmortem interval (PMI), there is a notable gap in understanding the postmortem preservation of germplasm in endangered species. <b><i>Objectives:</i></b> This study aimed to investigate the dynamics of apoptosis in various tissues of the Yangtze sturgeon at different postmortem time points, and to provide a reference for identifying the optimal time window for germplasm preservation in rare and endangered fish in the wild. <b><i>Methods:</i></b> Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphated nick-end labeling assay and tissue morphology analyses were used to investigate apoptosis in the brain, heart, fin, liver, gonad, muscle, spleen, and skin of the Yangtze sturgeon at five different time points 0, 4, 8, 12, and 16 hours postmortem. <b><i>Results:</i></b> The results revealed a dynamic pattern of apoptosis. All tissues exhibited a time-dependent increase in apoptotic rate, indicating a clear correlation between PMI and apoptosis progression. This temporal pattern underscores the importance of timely genetic resource preservation, as the integrity of genetic material deteriorates progressively after death. Histomorphological analysis further demonstrated progressive degradation of tissue structure, especially in metabolically active tissues such as the gonad and fin. <b><i>Conclusion:</i></b> Based on the findings, we recommend that the genetic resources of the Yangtze sturgeon be preserved as soon as possible after death, particularly within the first 12 hours when tissue integrity remains sufficient for viable cell isolation or cryopreservation. This window is critical for metabolically active tissues, which show marked changes over time and may be important for postmortem identification. Further research should explore cryopreservation and antioxidant treatments to extend the preservation window for germplasm resources, ensuring the long-term viability of these valuable genetic materials.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}