Biopreservation and Biobanking最新文献

{"title":"Application of Herbal-Based Antioxidants in Human Sperm Cryopreservation: A Systematic Review and Meta-Analysis Study.","authors":"Vahid Shokri-Asl, Rasa Khodavirdilou, Hamed Hajipour, Maryam Pashaiasl, Amir Fattahi","doi":"10.1177/19475535251359868","DOIUrl":"https://doi.org/10.1177/19475535251359868","url":null,"abstract":"<p><p>Sperm cryopreservation (SC) is an acceptable laboratory procedure for long-term storage of sperm. However, this procedure causes sperm damage. This review aimed to systematically investigate the effects of herbal-based antioxidants (HBAs) application on sperm parameters during SC. Following determination of the main keywords and searching strategy, various databases were searched systematically. Primary and secondary screenings were applied based on inclusion/exclusion criteria. Finally, 27 randomized controlled trial studies using 15 different HBAs and involving 557 normal and 130 abnormal semen samples were included for the meta-analysis. HBAs administration before or during the SC process improved total motility (mean difference [MD]: -6.31 [95% confidence interval [CI]: -10.27, -2.34], <i>p</i> < 0.05), viability (MD: -1.21 [95% CI: -1.52, -0.89], <i>p</i> < 0.001), morphology (MD: -1.72 [95% CI: -2.89, -0.54], <i>p</i> < 0.05), reactive oxygen species (H₂O₂) (MD: 7.58 [95% CI: 3.90, 11.26], <i>p</i> < 0.001), DNA integrity (MD: -13.21 [95% CI: -19.94, -6.49], <i>p</i> < 0.001), and sperm DNA fragmentation (SDF) (MD: 3.76 [95% CI: 2.38, 5.14], <i>p</i> < 0.001) after thawing in normal specimens. In abnormal semen samples, the HBAs improved viability (MD: -8.54 [95% CI: -11.18, -5.19], <i>p</i> < 0.001), progressive motility (MD: -4.55 [95% CI: -7.37, -1.73], <i>p</i> < 0.05) and ameliorated SDF (MD: 3.76 [95% CI: 1.74, 5.79], <i>p</i> < 0.001). Also, HBAs had no effects on total motility (MD: -0.08 [95% CI: -3.29, 3.14], <i>p</i> > 0.05), and morphology (MD: -0.31 [95% CI: -0.71, 0.09], <i>p</i> > 0.05). Application of HBAs in cryomedia before and during SC can improve sperm parameters (including viability, motility, and morphology), decrease oxidative stress and SDF levels.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thinking Outside the Individual Box-A Communitarian Approach to Bioethics for Biorepositories.","authors":"Elizabeth Sarah Mayne","doi":"10.1177/19475535251359865","DOIUrl":"https://doi.org/10.1177/19475535251359865","url":null,"abstract":"<p><p>Biorepositories in African populations are an important tool to ensure inclusive research to benefit African populations and the African diaspora. The establishment of biorepositories in Africa may, however, be impeded if culturally sensitive practices are not followed with respect to community engagement and informed consent. Communitarianism is a philosophical model which can be applied to African ethical processes. This model considers the individual within the broader context of the community and is compatible with cultural and religious practices in diverse African countries. This review considers the application of communitarianism to ethical and socially acceptable biorepository best practice on the African continent.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ISBER Corner: The ISBER 2025 Awards.","authors":"Alison Parry-Jones, Dayong Gao","doi":"10.1177/19475535251361070","DOIUrl":"https://doi.org/10.1177/19475535251361070","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144644202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Multimedia Electronic Consent Platform for Biobanking and Research Utilizing Opinions from Children, Teens, and Adults.","authors":"Iryna Kayda, Ashton Ellis, Vi Nguyen, Holly Longstaff, Elodie Portales-Casamar, Julie M Robillard, Alice Virani, Ashley McKerrow, Michelle Dittrick, George Lei, Suzanne Vercauteren","doi":"10.1089/bio.2024.0165","DOIUrl":"https://doi.org/10.1089/bio.2024.0165","url":null,"abstract":"<p><p><b><i>Objective:</i></b> Ethical research recruitment relies on effective informed consent. We sought feedback and acceptance from users regarding an interactive, multimedia, electronic consent (e-consent) platform for recruitment of research participants to the BC Children's Hospital BioBank (BCCHB). We aimed to enhance user experience when considering research participation and documenting consent decisions through the modality of an e-consent. <b><i>Study Design:</i></b> A prototype e-consent was developed and end-user opinions regarding content, visuals, user satisfaction, and electronic consent/assent practices were obtained from children, teens, and adults via an online survey and focus groups. A finalized e-consent was submitted for research ethics board (REB) approval. <b><i>Results:</i></b> All age groups rated the description of information, images, and formatting in the e-consent as highly favorable. Teens and adults preferred online (38% and 42%) rather than paper-based (17% and 16%) consent, while children expressed no preference. Majority of children (100%), teens (92%), and adults (98%) agreed or strongly agreed that they understood all the information given during the online consent process. No significant differences were found in survey responses between age groups. Adult and teen focus groups suggested improvements in formatting and addition of features to further clarify terms like \"ongoing donation\" and \"privacy measures.\" All ages preferred the ability to complete the e-consent independently, with optional assistance from research staff. The e-consent received REB approval and was implemented for BCCHB recruitment. <b><i>Conclusion:</i></b> An e-consent was developed and its modality was successfully accepted by end-users from several age groups, including children and teens, for use in pediatric biobanking. This method may potentially improve the process of completing research consent, particularly with adolescents.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Latin America: A Model for the Global Plant Cryopreservation Initiative.","authors":"David D Ellis, Rainer Vollmer, Fernanda V D Souza, Vânia C R Azevedo","doi":"10.1089/bio.2025.0067","DOIUrl":"https://doi.org/10.1089/bio.2025.0067","url":null,"abstract":"<p><p>Latin America (Mexico, the Caribbean, and Central and South America) is a megadiverse region. From Mexico and the Caribbean, through Central America to the Amazon and Andes, Latin America's botanical diversity contributes significantly to global food production and security, as it is the region of domestication for crops essential to human survival, including maize, potato, cassava, sweet potato, and beans. However, Latin America is also one of the most threatened regions with massive loss of native habitat where cultivars of these domesticated crops and their wild relatives, invaluable for new traits important for the adaptation to the abiotic and biotic challenges from climate change, currently exist. Many of these crops are vegetatively propagated, and their unique allelic makeup is crucial to conserve. Unfortunately, these unique genotypes cannot be conserved as seed and are at risk of vanishing forever due to the lack of secure conservation methods. The Global Plant Cryopreservation Initiative (GPCI) is addressing this challenge by providing a secure, long-term method for conserving plant diversity which cannot be conserved as botanical seed. The Latin America hub for the GPCI, at the International Potato Center (CIP) in Peru, is a model for how to mobilize and build capacity at the country level through national programs to conserve their genetic resources. Starting with a regional in-person workshop in 2022, CIP has maintained momentum for a plant cryopreservation network in Latin America by holding annual regional virtual cryopreservation meetings. CIP provided in-person capacity training for Latin American researchers in plant genetic resources conservation, with a special focus on cryopreservation. CIP has also implemented national plant cryopreservation programs in Ecuador and Chile, which include backing up their accessions in CIP's \"cryo-vault.\" These activities provide the foundation for the future of a strong, lasting plant cryopreservation community of practice throughout Latin America.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biobanks in Latin America: Insights from a Multinational Survey Conducted in 2021 During the COVID-19 Era.","authors":"Antonio Hugo José Fróes Marques Campos, María Teresa G de Dávila","doi":"10.1089/bio.2025.0064","DOIUrl":"https://doi.org/10.1089/bio.2025.0064","url":null,"abstract":"<p><p>Biobanks provide essential resources for research, yet little is known about their development in Latin America. This study reports the results of a cross-sectional survey conducted by the International Society for Biological and Environmental Repositories among Latin American biobanks between February and June 2021, during the second year of the COVID-19 pandemic. In total, 56 biobanks from seven countries responded to the survey, answering questions that included their institutional affiliations, funding, governance, and access policies. Most biobanks (94%) are linked to public or academic institutions, with human health research, particularly oncology, as the primary focus. Financial sustainability is a major concern, with most biobanks relying on institutional or grant-based funding. While many biobanks report adherence to international standard operating procedures and openness to share samples and associated data, challenges in data standardization and collaboration persist. Strengthening regional integration, harmonizing governance policies, and ensuring sustainable funding strategies are critical for enhancing the role of biobanks in Latin American and global research.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Robust, Inexpensive Solution for Alarm Monitoring of Freezers, Incubators, and Other Biobanks Equipment Based on Readily Available, Easy-to-Deploy Home Alarm Equipment.","authors":"Jonathan E Katz, Stella Somiari","doi":"10.1089/bio.2025.0032","DOIUrl":"https://doi.org/10.1089/bio.2025.0032","url":null,"abstract":"<p><p>Equipment monitoring (e.g., freezers, incubators) plays a vital role in specimen storage infrastructure. Monitoring solutions are often associated with a significant initial expense and short-term operational lifespan due to expensive contracts or suppliers going out of business. Numerous incidents of \"monitored\" lab equipment failing without notice have been reported. This has led to the loss of irreplaceable research material and severely impacted careers. In pursuit of a universally accessible solution, we deployed and now have over 15 years of continuous experience using residential alarm systems for laboratory monitoring. In this communication, it is presented how home alarm systems, designed for robust, fail-safe monitoring, can be used to augment commercially available solutions. Provided are high-level descriptions of how to deploy these solutions for equipment monitoring and how to expand their use into other monitoring tasks.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of N-Acetyl Cysteine on Human Post-Thaw Sperm Quality and Mitochondrial Uncoupling Protein 2 Relative Quantity.","authors":"Pegah Dorodian, Abdolhossein Shahverdi, AliReza Alizadeh, Leila Rashki Ghaleno, Shima Abbasihormozi, Vahid Esmaeili, Vahid Akbarinejad, Mohsen Sharafi","doi":"10.1089/bio.2024.0007","DOIUrl":"https://doi.org/10.1089/bio.2024.0007","url":null,"abstract":"<p><p>Reactive oxygen species (ROS) during cryopreservation causes mechanical, biochemical, and structural damage to the sperm, which leads to reduced sperm motility and fertility. N-acetyl cysteine is a cysteine-derived amino acid antioxidant that functions as a scavenger of ROS and regulates mitochondrial activity. Mitochondrial uncoupling protein 2 (UCP2) plays a leading role in this process and is one of the major regulators of human spermatozoa motility and metabolism. The purpose of the study was to examine the changes in UCP2 in frozen-thawed human sperm when exposed to N-acetyl cysteine, an effective antioxidant commonly used in human semen freezing. Semen samples were collected from 20 normozoospermia men and were divided into four experimental groups: fresh, frozen control, frozen N-Acetylcysteine (NAC, 100 μM), and frozen negative control with Genipin (25 μM). Subsequently, post-thaw sperm quality parameters, as well as UCP2 relative quantity, ROS, mitochondrial membrane potential (MMP), and malondialdehyde, were assessed. Semen treated with NAC exhibited significantly higher total and progressive motility, as well as viability, when compared to the control and genipin groups (<i>p</i> < 0.05). Moreover, UCP2 relative quantity was significantly lower in all frozen groups compared to the fresh group (<i>p</i> < 0.0001). The UCP2 relative quantity was not significantly different between NAC and control groups (<i>p</i> ≥ 0.05). Also, there were no significant differences in MMP, ROS, and malondialdehyde levels among the frozen groups (<i>p</i> ≥ 0.05). It can be concluded that UCP2 undergoes a modification during cryopreservation, and it could be an explanation of the reduction in post-thaw motility of sperm. Additionally, NAC supplementation in freezing media enhances post-thaw sperm motility and viability.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing Extracellular and Intracellular Antioxidants in Human Sperm Rapid Freezing: Hypotaurine Versus Melatonin.","authors":"Ali Zolfagharizadeh, Leila Rashki Ghaleno, Vahid Esmaeili, AliReza Alizadeh, Mojtaba Rezazadeh Valojerdi, Abdolhossein Shahverdi","doi":"10.1089/bio.2024.0133","DOIUrl":"https://doi.org/10.1089/bio.2024.0133","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Even with the significant advancements in sperm cryopreservation, the addition of intracellular or extracellular antioxidants in preparation and freezing media remains an understudied topic. <b><i>Objective:</i></b> We examined the effects of hypotaurine and melatonin on the routine and functional tests of sperm and the expression of <i>HspA2</i> and <i>Caspase9</i> during the human sperm rapid freezing process. <b><i>Methods:</i></b> Following the collection of 34 normospermia semen samples, each sample was split into four experimental groups: fresh (F), freezing control (C) (human tubal fluid medium and 0.5M sucrose), and two freezing groups with the inclusion of 2 mM melatonin (MEL) and 50 mM hypotaurine (HYP). A straw held 100 μL of the sample, which was then cryopreserved in liquid nitrogen to accomplish rapid freezing. Before and after rapid freezing-thawing, the sperm classical parameters and the expression levels of <i>HspA2</i> and <i>Caspase9</i> were assessed. <b><i>Results:</i></b> The HYP group exhibited higher normal morphology (<i>p</i> < 0.001), viability (<i>p</i> < 0.001), and higher acrosome integrity (<i>p</i> < 0.001) and lower DNA fragmentation index (DFI) (<i>p</i> < 0.001) than the C and MEL groups. No significant difference was observed in the total and progressive motility percentage among the antioxidant and frozen control groups. The MEL group had a significantly higher level of <i>HspA2</i> mRNA compared with F and C groups (<i>p</i> < 0.05). The expression of <i>Caspase9</i> was unaffected by including MEL and HYP in all experimental groups. <b><i>Conclusion:</i></b> Hypotaurine, as an extracellular antioxidant, is more effective than melatonin as an intracellular antioxidant in reducing deleterious cryoinjuries on morphology, viability, acrosome reaction, and DFI.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming Biobanking with AI: Perspectives from Leading Experts.","authors":"Ahmed Samir Abdelhafiz, Marianne K Henderson, Zisis Kozlakidis, Imon Banerjee, Gouri Mahajan, Weiye Charles Wang, Gregory H Grossman, Soichi Ogishima, Philip Quinlan","doi":"10.1089/bio.2025.0101","DOIUrl":"10.1089/bio.2025.0101","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}