Biopreservation and Biobanking最新文献

{"title":"Early Detection of Cryogenic Dewar Degradation Using LevelSense™: Longitudinal Temperature Analytics and Data-Driven Risk Stratification in IVF Cryostorage.","authors":"Michael Rusnack","doi":"10.1177/19475535261470253","DOIUrl":"https://doi.org/10.1177/19475535261470253","url":null,"abstract":"<p><strong>Background: </strong>Cryogenic Dewars used for IVF cryostorage are commonly evaluated through periodic inspections and evaporation measurements, which may delay the detection of insulation degradation or lead to premature Dewar retirement. Conventional failure models emphasize catastrophic vacuum loss; however, field observations suggest that many Dewars exhibit transient or progressive thermal anomalies prior to permanent failure.</p><p><strong>Methods: </strong>A retrospective observational study was conducted using longitudinal liquid nitrogen (LN₂) temperature telemetry data acquired from static IVF cryostorage units operating under continuous clinical conditions. Existing temperature data were evaluated to identify transient deviations, recovery behavior, event frequency, and long-term baseline drift. These features were integrated into a composite Dewar Health Index (DHI) designed to characterize insulation performance and degradation risk over time without direct measurement of vacuum pressure. In parallel, the normal evaporation rate, the steady-state rate of LN₂ evaporative loss derived from continuous level telemetry, was evaluated as an independent, energy-based measure of heat leak into the cryogenic system. Trend-based analytics were applied to distinguish temporary disturbances from progressive and permanent degradation modes. Analyses were performed on randomly sampled, de-identified telemetry drawn from an extensive cryostorage monitoring database comprising over 1000 active zones with continuous 5-minute data acquisition.</p><p><strong>Results: </strong>Cryostorage Dewars exhibited nonbinary thermal behavior, including transient temperature deviations followed by recovery, consistent with known cryogenic phenomena and dynamics. Dewars exhibiting increasing excursion frequency, prolonged recovery times, and sustained baseline temperature drift showed patterns consistent with progressive degradation prior to retirement. The DHI differentiated stable, degrading, and high-risk Dewars solely based on longitudinal temperature telemetry.</p><p><strong>Conclusions: </strong>Longitudinal temperature analytics provide a noninvasive means of assessing the health of cryogenic Dewars in IVF cryostorage applications. The DHI enables early identification of degradation trends and supports risk stratification and maintenance planning without changes to clinical workflows. These findings highlight the value of continuous monitoring and data-driven assessment for improving cryostorage reliability and safety in assisted reproductive technology environments.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261470253"},"PeriodicalIF":1.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biospecimen Science: The Foundation for Reliable and Reproducible Research.","authors":"Fay Betsou, Crystal M Kelly, Stella Somiari, Lalita Wadhwa","doi":"10.1177/19475535261481642","DOIUrl":"https://doi.org/10.1177/19475535261481642","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261481642"},"PeriodicalIF":1.4,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148820322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability of Reference Genes in Rat Ovarian Tissue Under Different Cryopreservation Conditions: A Multialgorithm Assessment.","authors":"Yixiong Guo, Peng Zhang, Kun Zhang, Qin Li, Qingfeng He, Wei Li, Lijun Liu, Haie Li, Bin Zhou, Xiaoming Zhou","doi":"10.1177/19475535261481925","DOIUrl":"https://doi.org/10.1177/19475535261481925","url":null,"abstract":"<p><strong>Background: </strong>Accurate quantitative real-time PCR (qPCR) normalization for cryopreserved ovarian tissue requires stable reference genes; however, published reports on the stability of such reference genes remain contradictory.</p><p><strong>Methods: </strong>The mRNA expression levels of nine candidate reference genes were quantified using reverse-transcription quantitative PCR (RT-qPCR) in a rat ovarian tissue model subjected to four conditions (fresh control, slow freezing, vitrification, liquid nitrogen snap freezing). Stability was assessed using four algorithms (NormFinder, geNorm, BestKeeper, and the ΔCt method), with emphasis on intergroup variation to identify genes most suitable for cross-protocol comparison.</p><p><strong>Results: </strong>Cryopreservation significantly affected the expression of all candidate genes (ANOVA, <i>p</i> < 0.05). Notably, expression levels were downregulated in the vitrification group (group effect: from -1.043 to -3.089) and upregulated in the slow freezing group (group effect: from + 0.939 to + 1.577). The composite RefFinder ranking identified <i>Rpl9</i> and <i>Tbp</i> as the most stable genes overall, whereas NormFinder identified <i>Ppia</i> (stability value <i>S</i> = 1.133) and <i>Ywhaz</i> (<i>S</i> = 1.176) as the optimal pair for cross-protocol comparison. Specifically, <i>Ppia</i> exhibited the smallest slow freezing group effect (+0.939), and <i>Ywhaz</i> showed the smallest liquid nitrogen snap freezing group effect (+0.283). geNorm analysis confirmed that two reference genes are sufficient for reliable normalization (pairwise variation <i>V2/3</i> = 0.108). <i>Hprt1</i> and <i>Gapdh</i> were consistently ranked as the least stable genes.</p><p><strong>Conclusion: </strong><i>Ppia</i> was most stable under slow freezing, while <i>Ywhaz</i> was most stable under liquid nitrogen snap freezing. Among the four conditions, the expression of the candidate genes was most stable under slow freezing, whereas the greatest transcriptional stress was observed under vitrification, making it a valuable model for cryopreservation damage assessment but less ideal for maintaining reference gene stability. This work provides a validated, purpose-built normalization tool for the rat ovarian cryopreservation model, enabling objective molecular evaluation and facilitating the optimization of cryopreservation techniques.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261481925"},"PeriodicalIF":1.4,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148820388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of the Ficoll-Paque and Miltenyi autoMACS^® PBMC Isolation Methods.","authors":"Hannah Damico, Rachael T C Sheridan, Alexander A Blanski, Martha L Escobar Galvis, Tori Evans, Bart O Williams, Scott D Jewell, Daniel C Rohrer","doi":"10.1177/19475535261479875","DOIUrl":"https://doi.org/10.1177/19475535261479875","url":null,"abstract":"<p><strong>Background: </strong>This study compared the isolation of peripheral blood mononuclear cells (PBMCs) using two methods. The first method was our routine Ficoll-Paque density gradient centrifugation isolation, which had been in place for many years. The second method was a new kit produced by Miltenyi on an autoMACS^® Pro Separator with the new human Whole Blood PBMC Isolation Kit. The goal was to determine the potential to replace the manual Ficoll-Paque method with this new semi-automated method to improve standardization and efficiency of PBMC isolation, a pivotal step in processing whole blood cells for our biobanking purposes.</p><p><strong>Methods: </strong>PBMCs were isolated from the peripheral blood of 10 healthy donors by routine Ficoll-Paque density gradient centrifugation and an autoMACS^® Pro Separator by Miltenyi. Isolated PBMCs were analyzed by flow cytometry, and the proportions of lymphocyte and monocyte subtypes were determined. In addition, a panel of 16 kinase inhibitors was used to determine differences in drug responses using an assay that determines cell death over time.</p><p><strong>Results and conclusion: </strong>Compared with Ficoll-Paque, the Miltenyi method yielded approximately 20 percent lower PBMC yield with no significant difference in cell viability and was superior when removing red blood cells. Proportions of some subpopulations (total T cells, B cells, and classical and intermediate monocytes) were altered based on the isolation method. Significant differences in drug responses were observed between the two methods. Moreover, the Miltenyi method introduced additional variability in drug sensitivity assays. These findings demonstrate that the two methods are not equivalent, which may limit the extent to which the Miltenyi method can improve standardization and reduce laboratory technician time in the isolation of PBMCs for biobanking.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261479875"},"PeriodicalIF":1.4,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital Biobanking in the Era of Artificial Intelligence.","authors":"Pranab Dey","doi":"10.1177/19475535261474367","DOIUrl":"https://doi.org/10.1177/19475535261474367","url":null,"abstract":"<p><p>The terminology \"Biobank\" is used for the organized collection of biological materials consisting of tissue samples, blood, serum, body fluids, and DNA/RNA materials for scientific research. A biobank extracts the information from the biospecimens to generate databases, which are continuously updated. Over the years, there have been significant advances in digital pathology and artificial intelligence (AI). Digital pathology primarily relies on whole-slide imaging and cloud computing. AI, on the contrary, is able to handle vast amount of data and to recognize the patterns of the histopathology images in more meaningful ways. A digital biobank is defined as the integration of the bio-specimens with whole-slide images, molecular data, longitudinal clinical information, and metadata. Digital biobanking can do real-time monitoring of the quality of the biospecimen, rapid retrieval of data, and AI-based research work. It may take a pivotal role in precision medicine by utilizing AI-based models for patient management. This review discusses the evolution from conventional to digital biobanking and emphasizes AI-based quality control, multimodal data integration, and predictive analysis in oncology. In addition, virtual biobanks, hybrid physical-virtual models, and digital twins are also discussed. The article also discusses the key challenges related to the implementation of digital biobanking, including standardization, interoperability, and sustainability.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261474367"},"PeriodicalIF":1.4,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Framework for Establishing a Specialized Biobank and Digital Information Platform for Skin-on-a-Chip Research in Plastic Surgery.","authors":"Xiuhong Li, Tianxiao Wei, Yun Zhang, Shan Zhu, Ningbei Yin, Nuo Si","doi":"10.1177/19475535261479035","DOIUrl":"https://doi.org/10.1177/19475535261479035","url":null,"abstract":"<p><strong>Background: </strong>High-quality primary cells and well-annotated clinical data are essential for reproducible skin-on-a-chip (SoC) research. However, conventional biobanking workflows often lack SoC-oriented procedures for specimen acquisition, live-cell preservation, quality control, and traceability.</p><p><strong>Methods: </strong>We established a specialized plastic surgery biobank incorporating standardized tissue procurement, cold-chain transport at 2°C-8°C with processing initiated within 2-6 hours, hierarchical preservation of tissues and live cells, and a unique identifier (UID)-linked multimodal digital platform. The biobank primarily archives skin tissue specimens, while a representative subset of specimens was processed for primary-cell isolation and quality-control evaluation. Post-thaw cell viability was assessed primarily by trypan blue exclusion, with flow cytometry used as an auxiliary method in representative batches. Passage-associated lineage-marker expression was evaluated by immunofluorescence.</p><p><strong>Results: </strong>The biobank currently archives 450 skin tissue specimens, of which samples from donors aged 0-14 years account for 63.8%. Among the representative cell-production batches evaluated, all available trypan blue image and counting records showed a mean post-thaw viability of 93.91 ± 3.37% (range, 88.24%-100.00%; coefficient of variation, 3.59%), with a cell count-weighted viability of 93.78%. P5 keratinocyte and fibroblast cultures showed near-complete K14 and vimentin positivity, respectively, whereas melanocyte melanocyte melan-A/melanoma antigen recognized by T-cells 1 (MLANA/MART-1) positivity was high at P1 (80.72 ± 11.67%) and decreased at P5 (38.41 ± 4.50%; Welch's <i>t-</i>test, <i>p</i> = 0.021). The operational digital platform supports UID-based sample management, sample-imaging association, storage management, and structured metadata export.</p><p><strong>Conclusion: </strong>This study presents a framework for establishing a fit-for-purpose and traceable specialized biobank and digital information platform to support plastic surgery SoC research. The current findings provide preliminary quality-control evidence for viable keratinocyte and fibroblast preparations and support the preferential use of early passage, marker-guided melanocyte preparations. Comprehensive functional SoC validation, longitudinal assessment of cellular stability, and melanocyte-specific preservation and release criteria require further investigation.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261479035"},"PeriodicalIF":1.4,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Building Better Biobanks: Role of Stakeholder Engagement.","authors":"Marianna Bledsoe, Amanda Rush","doi":"10.1177/19475535261481639","DOIUrl":"https://doi.org/10.1177/19475535261481639","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261481639"},"PeriodicalIF":1.4,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guideline for the Evaluation of Biobank Collections.","authors":"Joëlle C Schutten, Wim Timens, Peggy Manders","doi":"10.1177/19475535261479055","DOIUrl":"https://doi.org/10.1177/19475535261479055","url":null,"abstract":"<p><strong>Introduction: </strong>Biobank collections are a fundamental pillar for translational biomedical research and significantly contribute to the improvement of healthcare. To ensure the long-term continuity of these collections, it is essential to strike a balance between the financial, societal, and operational aspects of the collections.</p><p><strong>Methods: </strong>This paper provides advice for a guideline that outlines how to achieve this balance via periodic or interim evaluations of biobank collections by assessing their suitability for scientific research (\"Specimen and Data Considerations\") and their scientific potential (\"Institutional Value and Resource Considerations\").</p><p><strong>Results: </strong>The result of a periodic or interim evaluation is a combination of suitability (\"Specimen and Data Considerations\"-(meta)data, ethical-legal considerations, biological material) and the purpose of the biobank collection (\"Institutional Value and Resource Considerations\"-value, use, available resources). The presented guideline offers comprehensive directions and considerations to assist in making informed decisions regarding the retention, transfer, or termination of a biobank collection.</p><p><strong>Discussion: </strong>Regular evaluations of biobank collections are necessary to ensure their value for scientific research. A well-balanced approach ensures the continuous availability of sufficient, high-quality research biomaterials and associated data and their careful handling. This balance is important for advancing scientific progress, ultimately resulting in a better understanding of disease and new opportunities for prevention, diagnosis, and treatment.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261479055"},"PeriodicalIF":1.4,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementation of Catalase Enzyme in Extender Provides Antioxidative Protection to the Quality and Mitochondrial Activity of Frozen-Thawed Japanese Quail Spermatozoa.","authors":"Sumiyyah Zuha, Bushra Allah Rakha, Hajrah Raad Tihami, Shamim Akhter, Imtiaz Ahmad Khan","doi":"10.1177/19475535261468916","DOIUrl":"https://doi.org/10.1177/19475535261468916","url":null,"abstract":"<p><strong>Aim: </strong>Japanese quail offers massive potential as a source of animal protein and could fulfil the global surge in the demand for poultry meat. Sperm cryopreservation and assisted reproduction are pivotal in its propagation, as they simultaneously address various challenges like inbreeding, unreceptiveness towards new mates and unmanaged larger flocks. However, freezing procedures promote oxidative stress, disturb the redox homeostasis, and deteriorate sperm quality. Therefore, the current experiment was planned to evaluate the addition of catalase (CAT) antioxidant in the extender for Japanese quail sperm quality, mitochondrial activity, free radical scavenging activity and lipid peroxidation (LPO) during cryopreservation.</p><p><strong>Materials and methods: </strong>Semen was collected from 30 quails, pooled, divided and diluted with NaCl extender (115) containing 10 U CAT/mL, 30 U CAT/mL, 50 U CAT/mL and control. Extended semen (37°C) was gradually cooled to 20°C, then equilibrated at 4°C with 1% dimethylacetamide (DMA) and cryopreserved in liquid nitrogen. The sperm quality and biochemical parameters were assessed at the post-dilution, post-cooling, post-equilibration and post-thaw (0-hour and half-hour) stages of cryopreservation.</p><p><strong>Results: </strong>The sperm motility, viability, plasma membrane and acrosomal integrity, mitochondrial activity and free radical scavenging activity were documented as highest (<i>p</i> < 0.05) with 10 U CAT/mL compared to other concentrations and the control at all the stages of cryopreservation. The LPO in sperm and seminal plasma was recorded lowest (<i>p</i> < 0.05) with 10 U CAT/mL at all the freezing stages. Moreover, at the post-thaw half-hour stage, only 10 U CAT/mL maintained the sperm quality.</p><p><strong>Conclusion: </strong>The addition of 10 U CAT/mL efficiently preserved and protected the sperm plasma membrane, acrosome, and mitochondrial activity as an antioxidative agent against the free radicals produced at chilling temperatures.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261468916"},"PeriodicalIF":1.4,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148762698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Nonhuman Primate Developmental Genotype-Tissue Expression Biospecimen and Histopathology Resource.","authors":"Jenna N Castro, Samia Silva de Castro, Rebecca L Linn, Patrick M Van Hoose, Lisa Anderson, Kristin G Ardlie, Donald F Conrad, Anne D Lewis, Victoria H J Roberts","doi":"10.1177/19475535261475862","DOIUrl":"https://doi.org/10.1177/19475535261475862","url":null,"abstract":"<p><strong>Introduction: </strong>The NIH-funded initiative Developmental Genotype-Tissue Expression (dGTEx) is an ongoing large resource of nonhuman primate (NHP) samples in which multiple organs are being collected, subdissected and preserved to build a unique tissue biorepository. Primates share close evolutionary relationships with humans, allowing for strong translation of research findings to human health.</p><p><strong>Objective: </strong>Generate a resource consisting of the two most frequently used NHP species in research the rhesus macaque (<i>Macaca mulatta</i>), which is widely available and has a well-studied genome, and the common marmoset (<i>Callithrix jacchus</i>), which is favored for studies of brain development and neurodegenerative disease.</p><p><strong>Methods: </strong>The dGTEx project is building (1) a genetic repository that will provide genome and tissue-based RNA sequencing for these Old and New World primate species to identify how genetic variations affect normal gene expression and to understand mechanisms of disease, and (2) an expansive histopathology resource of preserved individual organs. Detailed animal history, demographics, and sample metadata are documented in a secure database. Samples are being obtained from first (rhesus only), second, and third trimester fetal cohorts (with accompanying gestational tissues) as well as neonatal, infant, juvenile, and adult animal timepoints balanced for male and female to facilitate sex-specific data analysis.</p><p><strong>Results: </strong>We detail the sampling sites, preservation methods, and accompanying histology review that allows for confirmation of tissue type and quality, in addition to noting any pathological findings.</p><p><strong>Conclusion: </strong>This comprehensive NHP resource enables developmental investigations across the lifespan, from prenatal and postnatal stages through to adulthood.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"19475535261475862"},"PeriodicalIF":1.4,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148686771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}