Biopreservation and Biobanking最新文献

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Transforming Biobanking with AI: Perspectives from Leading Experts. 用人工智能改造生物银行:来自领先专家的观点。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-19 DOI: 10.1089/bio.2025.0101
Ahmed Samir Abdelhafiz, Marianne K Henderson, Zisis Kozlakidis, Imon Banerjee, Gouri Mahajan, Weiye Charles Wang, Gregory H Grossman, Soichi Ogishima, Philip Quinlan
{"title":"Transforming Biobanking with AI: Perspectives from Leading Experts.","authors":"Ahmed Samir Abdelhafiz, Marianne K Henderson, Zisis Kozlakidis, Imon Banerjee, Gouri Mahajan, Weiye Charles Wang, Gregory H Grossman, Soichi Ogishima, Philip Quinlan","doi":"10.1089/bio.2025.0101","DOIUrl":"https://doi.org/10.1089/bio.2025.0101","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Saliva Biobanking: A Scoping Review of Collection, Processing, and Storage Practices to Enhance Diagnostic Potential. 唾液生物银行:收集,处理和储存实践的范围审查,以提高诊断潜力。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-16 DOI: 10.1089/bio.2025.0043
Manuela da Silva Spinola, Danielle Viana Ribeiro, Giulia Beletato Nery, David D Fischer, Nailê Damé-Teixeira, Débora Heller
{"title":"Saliva Biobanking: A Scoping Review of Collection, Processing, and Storage Practices to Enhance Diagnostic Potential.","authors":"Manuela da Silva Spinola, Danielle Viana Ribeiro, Giulia Beletato Nery, David D Fischer, Nailê Damé-Teixeira, Débora Heller","doi":"10.1089/bio.2025.0043","DOIUrl":"https://doi.org/10.1089/bio.2025.0043","url":null,"abstract":"<p><p><b><i>Objectives:</i></b> This study aimed to systematically map the literature on saliva collection, processing, and storage methods for biobanking purposes, identifying current practices and gaps in standardization. <b><i>Materials and Methods:</i></b> A systematic search was conducted in five electronic databases and gray literature. Original research articles reporting cross-sectional and longitudinal studies using saliva for biobanking, as well as reports and protocols for biobank establishment, were included. The review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews guidelines, with two reviewers independently assessing eligibility and extracting data. <b><i>Results:</i></b> A total of 50 articles were included, revealing that most studies were reports on biobank establishment (38%), followed by longitudinal studies (28%), protocols (20%), and cross-sectional studies (14%). Saliva collection methods varied, with commercial kits being the most reported (40%), followed by sterile tubes (22%). While 64% of studies described processing methods and 78% reported storage methods, significant gaps in reporting were noted. Unstimulated saliva was the most commonly collected sample type (38%), and most studies focused on adult participants (46%), often with disease conditions (36%). However, many studies did not provide sufficient details on collection methods, processing techniques, storage conditions, or participant demographics. <b><i>Conclusion:</i></b> This review highlights the urgent need for standardized protocols in saliva biobanking to ensure consistency, reliability, and reproducibility in diagnostic research. The lack of uniformity in methodologies across studies limits the potential of saliva biobanks as a resource for identifying biomarkers of systemic and oral diseases. Establishing harmonized guidelines will enhance international collaboration, enable robust analyses, and maximize the utility of saliva in advancing personalized medicine and public health.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144303687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lyophilization and Field Survival of Epiphytic Yeasts from the Phyllosphere of Native Cacao in the Peruvian Amazon. 秘鲁亚马逊地区原生可可叶层中附生酵母的冻干和野外存活。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-16 DOI: 10.1089/bio.2024.0185
Eryka Gaslac-Zumaeta, Kelvin James Llanos-Gómez, Segundo Manuel Oliva-Cruz, Jorge Ronny Díaz-Valderrama
{"title":"Lyophilization and Field Survival of Epiphytic Yeasts from the Phyllosphere of Native Cacao in the Peruvian Amazon.","authors":"Eryka Gaslac-Zumaeta, Kelvin James Llanos-Gómez, Segundo Manuel Oliva-Cruz, Jorge Ronny Díaz-Valderrama","doi":"10.1089/bio.2024.0185","DOIUrl":"https://doi.org/10.1089/bio.2024.0185","url":null,"abstract":"<p><p>Epiphytic yeasts are promising biocontrol agents of plant diseases but preserving and transferring them to the field is challenging. Here, we studied six cost-effective lyophilization protective agents to preserve seven strains of Amazonian yeast species isolated from the phyllosphere of native cacao (<i>Theobroma cacao</i>) in Peru. We evaluated the viability of yeasts at 30 and 90 days post-lyophilization <i>in vitro</i>, and their survival after controlled inoculation on cacao fruits in the field. The best protective agents were maltodextrin, honey + skim milk, and honey. <i>Wickerhamomyces anomalus</i> KLG-014 and <i>Wickerhamomyces</i> sp. EGZ-38 showed higher than 97.3% viability after 30 days when lyophilized with maltodextrin. Additionally, <i>Candida</i> sp. KLG-103 showed a viability greater than 50% after 30 days when lyophilized with honey + skim milk. At 90 days, <i>W. anomalus</i> KLG-014, <i>Hannaella theobromatis</i> KLG-063, and <i>Kwoniella heveanensis</i> EGZ-07 showed a viability greater than 20%, with the latter showing an outstanding 100% viability, when lyophilized with honey + skim milk. Conversely, sodium alginate was the least protective agent, as yeast showed 0% viability. In the field, <i>W. anomalus</i> KLG-014, <i>K. heveanensis</i> EGZ-07, <i>Debaryomyces hansenii</i> EGZ-31, and <i>Wickerhamomyces</i> sp. EGZ-38 were successfully re-isolated from the surface of cacao fruits under all treatments after 30 days, except for sodium alginate. This was corroborated via morphological and molecular evidence. This study demonstrates that maltodextrin, honey, and skim milk are suitable for ensuring the <i>in vitro</i> viability of biocontrol yeasts up to 90 days after lyophilization, and their survival up to 30 days after inoculation on cacao fruits in the field. This is a first step toward the development of a biocontrol alternative to mitigate cacao pathogens using native microorganisms from the Amazon in Peru.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144303686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Caffeic Acid Alleviates Oxidative Stress and Prolongs the Shelf-Life of Rat Platelets. 咖啡酸可减轻大鼠血小板氧化应激并延长其保质期。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-05 DOI: 10.1089/bio.2025.0039
Magdaline Christina Rajanand, Anusha Berikai Ananthakrishna, Vani Rajashekaraiah
{"title":"Caffeic Acid Alleviates Oxidative Stress and Prolongs the Shelf-Life of Rat Platelets.","authors":"Magdaline Christina Rajanand, Anusha Berikai Ananthakrishna, Vani Rajashekaraiah","doi":"10.1089/bio.2025.0039","DOIUrl":"https://doi.org/10.1089/bio.2025.0039","url":null,"abstract":"<p><p><b><i>Background:</i></b> Oxidative stress (OS) is one of the major contributors to platelet storage lesions. Addition of antioxidant additives to storage solutions can mitigate oxidative damage to platelets. Caffeic acid (CA) is a polyphenolic compound that directly scavenges reactive oxygen species (ROS), inhibits lipid peroxidation, and modulates platelet function signaling pathways. This is the first study to investigate the influence of CA on stored platelets. <b><i>Methodology:</i></b> Platelets obtained from the blood of male <i>Wistar</i> rats (<i>n</i> = 5 per group) were resuspended in SSP+ combined with plasma at 70:30 ratio. ROS was analyzed during a 7-day storage period for different concentrations of CA. The concentration of CA that exhibited maximum inhibition was chosen for further studies. Platelets (<i>n</i> = 5) were divided into (1) controls and (2) CA and stored at 22°C under mild agitation for 11 days. The markers of platelet function, viability, OS, and antioxidant defenses were analyzed. <b><i>Results:</i></b> CA at 5 µM concentration (5-CA) showed maximum inhibition of ROS on day 7; hence, was chosen for further assays. 5-CA augmented antioxidant defenses, decreased lipid peroxidation, and scavenged superoxide radicals compared with controls. Decline in platelet activation, aggregation without collagen, and microbial contamination were also observed in 5-CA. Platelet viability and metabolism were maintained; lactate dehydrogenase decreased by the end of storage in 5-CA. <b><i>Conclusion:</i></b> 5-CA in SSP+ could preserve the quality of stored platelets until day 7 of storage. This study emphasizes the potential of CA as an additive in platelet storage solutions in prolonging the shelf-life of platelets and maintaining their efficacy.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recognizing the Need to Connect: Encouraging Interdisciplinary Engagement with the International Society for Biological and Environmental Repositories. 认识到联系的需要:鼓励与国际生物和环境资源库协会的跨学科合作。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-05 DOI: 10.1089/bio.2025.0063
Debra Ellisor, Anna Bryan, Barbara Mahoney
{"title":"Recognizing the Need to Connect: Encouraging Interdisciplinary Engagement with the International Society for Biological and Environmental Repositories.","authors":"Debra Ellisor, Anna Bryan, Barbara Mahoney","doi":"10.1089/bio.2025.0063","DOIUrl":"https://doi.org/10.1089/bio.2025.0063","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Developing Informed Consent for Academic Hospital-Based Biobank Modeling: An Experience from Indonesia. 为学术医院生物库建模制定知情同意书:印度尼西亚的经验。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-01 Epub Date: 2024-08-21 DOI: 10.1089/bio.2024.0001
Wika Hartanti, Amirah Ellyza Wahdi, Tika Prasetiawati, Qurry Amanda Izhati, Jajah Fachiroh
{"title":"Developing Informed Consent for Academic Hospital-Based Biobank Modeling: An Experience from Indonesia.","authors":"Wika Hartanti, Amirah Ellyza Wahdi, Tika Prasetiawati, Qurry Amanda Izhati, Jajah Fachiroh","doi":"10.1089/bio.2024.0001","DOIUrl":"10.1089/bio.2024.0001","url":null,"abstract":"<p><p><b><i>Background:</i></b> Informed consent (IC) for biobank practice is vital to ensure that sample collection, storage, and utilization are ethical. However, the standard practices in biobanking in upper-middle-income countries such as Indonesia often rely on specific consent, leading to restricted sample use and ethical concerns. This article describes the development of an IC model that meets ethical standards and yet is acceptable for biobanking practice in an Indonesian academic hospital. <b><i>Method:</i></b> We conducted a study involving Universitas Gadjah Mada (UGM) Biobank Unit and the UGM Academic Hospital, Yogyakarta, Indonesia, between 2019 and 2021. The IC development process consisted of four stages: (1) conceptualization, (2) preparation, (3) pilot, and (4) evaluation. These activities were part of a more extensive pilot study for an academic hospital-based biobank (Medical Biobank for Research in Indonesia (MBRIO) study). <b><i>Result:</i></b> We conceptualized a broad consent model, consisting of an information sheet, comprehension test, agreement sheet, and exit survey. We tested and revised the broad consent document to ensure readability, trained 10 consenting staff (1 surgeon and 9 nurses), and then piloted the IC procedure on 24 patients with elective surgery. The evaluation showed that patients understood the information objectively and subjectively. Consenting staff considered the broad consent model acceptable for the academic hospital setting and suggested improvements to increase the readability of information sheets and have more trained staff for better coordination. <b><i>Conclusion:</i></b> The IC development process and model consent are ethically sufficient, acceptable and feasible to be implemented in academic hospital-based biobanks in Indonesia adjusted to the business processes.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"186-194"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142010002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Institutional Conversion to Energy-Efficient Ultra-Low Freezers Decreases Carbon Footprint and Reduces Energy Costs. 机构改用高能效超低冷冻机可减少碳足迹并降低能源成本。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-01 Epub Date: 2024-09-25 DOI: 10.1089/bio.2024.0070
Dean Shehu, Mi-Ok Kim, John Rosendo, Nevan Krogan, David O Morgan, B Joseph Guglielmo
{"title":"Institutional Conversion to Energy-Efficient Ultra-Low Freezers Decreases Carbon Footprint and Reduces Energy Costs.","authors":"Dean Shehu, Mi-Ok Kim, John Rosendo, Nevan Krogan, David O Morgan, B Joseph Guglielmo","doi":"10.1089/bio.2024.0070","DOIUrl":"10.1089/bio.2024.0070","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> The storage of biospecimens is a substantial source of greenhouse gas emissions and institutional energy costs. Energy-intensive ultra-low temperature (ULT) freezers used for biospecimen storage are a significant source of carbon emissions. ENERGY STAR-certified ULT freezers have the potential to decrease the carbon footprint. <b><i>Objective:</i></b> Quantify the impact of an institutional-scale freezer conversion program on carbon emissions and energy costs. <b><i>Methods:</i></b> A ULT freezer energy use prediction model was developed to identify and replace the most inefficient freezers in the research building for this pilot, and eventually institution-wide. Multiple linear regression factors included the number of years of use, storage volume, and ENERGY STAR certification status. Electrical usage and carbon emissions were quantified before and after replacement with ENERGY STAR models. Logistical methods were developed to decrease the risks of exposure of frozen samples to ambient temperature during content transfers. Institution-wide energy costs were derived by converting electrical burden to electrical costs. Carbon footprint assessment from ULT freezer operation was computed using the U.S. EPA Greenhouse Gas Equivalencies Calculator. <b><i>Results:</i></b> The pilot project revealed an annual reduction of 310,493 kilowatt hours of electrical usage, equivalent to 134 metric tons of carbon emissions. Annual electrical costs were reduced by $55,889 resulting in an 8-year payback on the initial investment. Using the pilot results, we modeled the benefit of the freezer exchange across the entire institution. The modeling predicted that conversion of the institution's remaining 1119 conventional ULT freezers to ENERGY STAR models would lower annual electrical usage by 7,911,549 kilowatt hours (3423 metric tons of carbon emissions), resulting in savings of over $1.4 million annually. <b><i>Conclusion:</i></b> Our methods make a large-scale initiative to replace energy-inefficient ULT freezers logistically possible, reduce carbon footprint, and demonstrate an attractive return on investment while proactively protecting valuable research materials.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"195-202"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Update on the Qualification in Biorepository Science Program at 5 Years. 五年制生物库科学专业资格认定的新进展。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-01 Epub Date: 2025-05-15 DOI: 10.1089/bio.2025.0100
Brent Schacter, Daniel Simeon-Dubach
{"title":"An Update on the Qualification in Biorepository Science Program at 5 Years.","authors":"Brent Schacter, Daniel Simeon-Dubach","doi":"10.1089/bio.2025.0100","DOIUrl":"10.1089/bio.2025.0100","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"248-250"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
International Regulatory Harmonization: One World for Human Health. 国际监管协调:同一个世界,为了人类健康。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-01 Epub Date: 2025-05-22 DOI: 10.1089/bio.2025.0102
Annette Schmid, Marianna Bledsoe
{"title":"International Regulatory Harmonization: One World for Human Health.","authors":"Annette Schmid, Marianna Bledsoe","doi":"10.1089/bio.2025.0102","DOIUrl":"10.1089/bio.2025.0102","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"163-164"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curcumin Protects Human Sperm Quality During Cryopreservation. 姜黄素在冷冻保存过程中保护人类精子质量。
IF 1.6 4区 生物学
Biopreservation and Biobanking Pub Date : 2025-06-01 Epub Date: 2025-02-10 DOI: 10.1089/bio.2023.0091
Banafsheh Mansouri-Bahrani, Maryam Azizi Kutenaee, Mitra Heydari Nasrabadi, Ensieh Salehi
{"title":"Curcumin Protects Human Sperm Quality During Cryopreservation.","authors":"Banafsheh Mansouri-Bahrani, Maryam Azizi Kutenaee, Mitra Heydari Nasrabadi, Ensieh Salehi","doi":"10.1089/bio.2023.0091","DOIUrl":"10.1089/bio.2023.0091","url":null,"abstract":"<p><p><b><i>Background:</i></b> Cryopreservation causes harmful effects on sperm quality due to reactive oxygen species (ROS) overproduction and physical-chemical modifications, resulting in reduced sperm fertility potential. Recently, many studies have shown that adding antioxidants to the cryopreservation medium can markedly reduce these damages. The present study aimed to evaluate the effects of pre-treatment with curcumin at 0, 20, 50, and 100 μM concentrations on frozen-thawed human sperm parameters. <b><i>Methods:</i></b> Semen samples from 25 normozoospermic men were collected. Then, each sample was divided into five equal parts: fresh group and frozen-thawed groups, including 0, 20, 50, and 100 μM of curcumin. Pre-cryopreservation and post-thaw sperm motility, morphology, vitality, DNA fragmentation, and ROS levels were investigated. <b><i>Results:</i></b> Cryopreservation significantly reduced sperm quality. A known value of 50 μM curcumin significantly improved sperm progressive motility (18.67 ± 1.12 vs. 11.2 ± 1.24, <i>p</i> < 0.01), vitality (35.50 ± 1.63 vs. 21.83 ± 2.64, <i>p</i> < 0.05), and decreased ROS levels (<i>p</i> < 0.05), 50 μM curcumin also efficiently preserved sperm morphology after thawing (13.55 ± 0.33 vs. 6.56 ± 0.16, <i>p</i> < 0.001). Furthermore, the application of 50 μM curcumin resulted in a reduction in DNA fragmentation, though it did not reach statistical significance (<i>p</i> = 0.08). In contrast, 20 μM curcumin only had a significant impact on progressive motility (15.85 ± 0.7 vs. 11.2 ± 1.24, <i>p</i> < 0.05), whereas, in the 100 μM group, there were no significant differences in any of the measured parameters compared with the control group. <b><i>Conclusion:</i></b> It seems that curcumin ameliorates cryopreservation-induced injury to sperm.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"236-242"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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