Biopreservation and Biobanking最新文献

{"title":"Effect of Moderate Static Magnetic Fields on Mice Oocyte Vitrification: Calcium-Related Genes Expression.","authors":"Sara Soleimani Pargoo, Farzaneh Baniasadi, Vida Sadat Kazemein Jasemi, Samira Hajiaghalou, Mohsen Gharanfoli, Rouhollah Fathi","doi":"10.1089/bio.2022.0200","DOIUrl":"10.1089/bio.2022.0200","url":null,"abstract":"<p><p>The ability to cryopreserve oocytes without ultrastructural injury has been a concern in the development and use of methods to preserve female reproduction. The stability of the cell membrane must be preserved to reduce the damage caused by ice crystals during vitrification. One approach that has been explored is the use of static magnetic fields (SMFs), which are believed to influence cell membrane stability. In this study, the <i>in vitro</i> effects of SMF that range between 20-80 mT on the vitrification of mice germinal vesicle (GV) oocytes were studied. The viability and mitochondrial (Mt) membrane potential of both vitrified and nonvitrified oocytes were assessed using Trypan blue and JC1 staining. The high <i>in vitro</i> maturation (IVM) rate and high Mt membrane potential in metaphase II (MII) oocytes were taken into account to determine the optimal magnetic field intensity, that is, 20 mT. None of the SMF conditions resulted in intact spindles in MII oocytes. The study also explored the expression of store-operated calcium entry (<i>Stim1</i>, <i>Orai1</i>, and <i>Ip3r</i>) and meiosis resumption (<i>Ccnb</i>, <i>Cdk</i>) genes in GV and MII oocytes of both vitrified and control groups. The results show that the expressions of <i>Orai1</i> and <i>Ccnb</i> genes in Vit-MII-SMF oocytes were considerably increased. However, no significant difference in <i>Stim1</i> expression was observed between the groups. The Vit-MII-SMF group exhibited a significantly higher <i>Ccnb</i> expression compared to other groups. <i>In vitro</i> fertilization (IVF) was performed to evaluate the 2 pronuclear (2PN) rates. The findings demonstrated that using 20 mT SMF improved 2PN rates compared to the nonvitrified groups. This study provides a deeper understanding of the effects of moderate SMF and vitrification on the expression of calcium channel genes in GV and MII oocytes. The results suggest that applying a 20 mT SMF can help prevent cryoinjury and enhance the characteristics of vitrified-warmed oocytes.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140289770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosalind Franklin Society Proudly Announces the 2023 Award Recipient for <i>Biopreservation and Biobanking</i>.","authors":"Emily Higgs","doi":"10.1089/bio.2024.93645.rfs2023","DOIUrl":"https://doi.org/10.1089/bio.2024.93645.rfs2023","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142481498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experts Speak Forum: Community Engagement in Research Biobanking.","authors":"Carol J Weil, Sandra Nanyonga, Azure Hermes, Alice McCarthy, Marielle Gross, Hellen Nansumba, Io Hong Cheong, Rubi-Jayne Cohen","doi":"10.1089/bio.2024.0131","DOIUrl":"https://doi.org/10.1089/bio.2024.0131","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142481497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Support for Research Biobanks Among Medical Students in the Republic of Kazakhstan.","authors":"Dariusz Walkowiak, Alua Miraleyeva, Tamara Batyrova, Jan Domaradzki","doi":"10.1089/bio.2023.0114","DOIUrl":"10.1089/bio.2023.0114","url":null,"abstract":"<p><p><b><i>Background:</i></b> Over the past few years, several biobanks have been organized in the Republic of Kazakhstan (RoK). As research biobanks are becoming an increasingly important tool for precision medicine, they require competent biobankers who will help disseminate the idea of biobanking throughout the society and support donation for research purposes. This study aimed to assess the support for research biobanks among medical students (MSs) in Kazakhstan. <b><i>Methods:</i></b> This research includes data from an anonymous, self-administered online questionnaire regarding MSs' support for research biobanks in the RoK. <b><i>Results:</i></b> Only 212 (31.3%) MSs have heard of biobanks and 466 (68.7%) have not. Students who were biobank-aware held more positive views regarding biobanks, were more likely to support the idea of establishing a research biobank in Kazakhstan, and expressed a greater willingness to donate their biological material for research purposes. Students' awareness of biobanks was also associated with the respondents' motivations to donate. Students who were familiar with biobanks tended to be motivated by altruistic reasons more often, and those unfamiliar with biobanks tended to indicate selfish motives. Students' attitudes toward donation to various types of research and biobanks were influenced by their awareness of biobanks, any family history of genetic disease, experience of chronic disease, declared religiousness, hospitalization history, blood donor status, and year of study. <b><i>Conclusion:</i></b> Since MSs' awareness of research biobanks is low, there is an urgent need for biobank education in Kazakhstan.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140289771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Institutional Conversion to Energy-Efficient Ultra-Low Freezers Decreases Carbon Footprint and Reduces Energy Costs.","authors":"Dean Shehu, Mi-Ok Kim, John Rosendo, Nevan Krogan, David O Morgan, B Joseph Guglielmo","doi":"10.1089/bio.2024.0070","DOIUrl":"https://doi.org/10.1089/bio.2024.0070","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> The storage of biospecimens is a substantial source of greenhouse gas emissions and institutional energy costs. Energy-intensive ultra-low temperature (ULT) freezers used for biospecimen storage are a significant source of carbon emissions. ENERGY STAR-certified ULT freezers have the potential to decrease the carbon footprint. <b><i>Objective:</i></b> Quantify the impact of an institutional-scale freezer conversion program on carbon emissions and energy costs. <b><i>Methods:</i></b> A ULT freezer energy use prediction model was developed to identify and replace the most inefficient freezers in the research building for this pilot, and eventually institution-wide. Multiple linear regression factors included the number of years of use, storage volume, and ENERGY STAR certification status. Electrical usage and carbon emissions were quantified before and after replacement with ENERGY STAR models. Logistical methods were developed to decrease the risks of exposure of frozen samples to ambient temperature during content transfers. Institution-wide energy costs were derived by converting electrical burden to electrical costs. Carbon footprint assessment from ULT freezer operation was computed using the U.S. EPA Greenhouse Gas Equivalencies Calculator. <b><i>Results:</i></b> The pilot project revealed an annual reduction of 310,493 kilowatt hours of electrical usage, equivalent to 134 metric tons of carbon emissions. Annual electrical costs were reduced by $55,889 resulting in an 8-year payback on the initial investment. Using the pilot results, we modeled the benefit of the freezer exchange across the entire institution. The modeling predicted that conversion of the institution's remaining 1119 conventional ULT freezers to ENERGY STAR models would lower annual electrical usage by 7,911,549 kilowatt hours (3423 metric tons of carbon emissions), resulting in savings of over $1.4 million annually. <b><i>Conclusion:</i></b> Our methods make a large-scale initiative to replace energy-inefficient ULT freezers logistically possible, reduce carbon footprint, and demonstrate an attractive return on investment while proactively protecting valuable research materials.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MIBLood-EV: An Online Reporting Tool to Facilitate the Standardized Reporting of Preanalytical Variables and Quality Control of Plasma and Serum to Enhance Rigor and Reproducibility in Liquid Biopsy Research.","authors":"Dakota Gustafson, Rienk Nieuwland, Fabrice Lucien","doi":"10.1089/bio.2024.0083","DOIUrl":"https://doi.org/10.1089/bio.2024.0083","url":null,"abstract":"<p><p>Pre-analytical variability significantly impacts the reproducibility of liquid biopsy research, which is critical for precision medicine and biomedical research. This report highlights the challenges and variability in the pre-analytical processes of liquid biopsies, especially regarding extracellular vesicles (EVs), which are crucial for diagnostics in oncology. The MIBlood-EV initiative aims to standardize the reporting of pre-analytical variables and the quality control of plasma and serum samples to enhance reproducibility in EV research. By providing a comprehensive and flexible reporting framework, MIBlood-EV seeks to improve the reliability of EV studies and facilitate the development of evidence-based protocols, ultimately advancing the field of liquid biopsy research.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142156678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Regulatory Frameworks for Biobanking in Southeast Asia.","authors":"Plebeian B Medina, Subasri Armon, Mohammad Firdaus Bin Abdul Aziz, Io Hong Cheong, Marian P de Leon, Sonia Drobysz, Muhd Haziq Fikry Bin Haji Abdul Momin, Debra Leiolani Garcia, Diah Iskandriati, Zisis Kozlakidis, Lin Cui, Seanghorn Mao, Mary Elizabeth Miranda, Khin Mar Mya, Lingeswran Nallenthiran, Marie Christine Obusan, Kongchay Phimmakong, Phyu Sabai, Channada Saejung, Hans Prakash Sathasivam, Faizatul Lela Binti Jafar, Rodel Jonathan S Vitor, Ailyn M Yabes, Alan B Calaor, Viji Vijayan, Raymond T P Lin","doi":"10.1089/bio.2024.0044","DOIUrl":"https://doi.org/10.1089/bio.2024.0044","url":null,"abstract":"<p><p>Southeast Asian countries are at the forefront of public health pressures due to a confluence of factors such as population growth, urbanization, environmental pollution, and infectious diseases (re)emergence. Therefore, the ability to be able to conduct research addressing local and regional needs is of paramount importance. As such, biobanking activities, the standardized collection of biological samples, and associated data, developed over the past few decades supporting ongoing biomedical and clinical research, as well as surveillance are of critical importance. However, the regulatory landscape of biobanking is not widely understood and reported, which this narrative review aims to address for the ASEAN member states. It is evident that there are specific regulatory arrangements within each ASEAN member state, which though may be sufficient for the current level of operations, are unlikely to support a regional sharing of biological samples, data, and eventually benefits from the conducted research. Additionally, legacy and often-overlapping regulatory frameworks exist, which raise the need of an eventual consolidation under a single framework. Thus, this field requires further study as well as the creation of viable, practical proposals that would allow for biobanking harmonization and thus the exchange of biological samples and data to be achieved regionally, if not further afield.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142156677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing Informed Consent for Academic Hospital-Based Biobank Modeling: An Experience from Indonesia.","authors":"Wika Hartanti, Amirah Ellyza Wahdi, Tika Prasetiawati, Qurry Amanda Izhati, Jajah Fachiroh","doi":"10.1089/bio.2024.0001","DOIUrl":"https://doi.org/10.1089/bio.2024.0001","url":null,"abstract":"<p><p><b><i>Background:</i></b> Informed consent (IC) for biobank practice is vital to ensure that sample collection, storage, and utilization are ethical. However, the standard practices in biobanking in upper-middle-income countries such as Indonesia often rely on specific consent, leading to restricted sample use and ethical concerns. This article describes the development of an IC model that meets ethical standards and yet is acceptable for biobanking practice in an Indonesian academic hospital. <b><i>Method:</i></b> We conducted a study involving Universitas Gadjah Mada (UGM) Biobank Unit and the UGM Academic Hospital, Yogyakarta, Indonesia, between 2019 and 2021. The IC development process consisted of four stages: (1) conceptualization, (2) preparation, (3) pilot, and (4) evaluation. These activities were part of a more extensive pilot study for an academic hospital-based biobank (Medical Biobank for Research in Indonesia (MBRIO) study). <b><i>Result:</i></b> We conceptualized a broad consent model, consisting of an information sheet, comprehension test, agreement sheet, and exit survey. We tested and revised the broad consent document to ensure readability, trained 10 consenting staff (1 surgeon and 9 nurses), and then piloted the IC procedure on 24 patients with elective surgery. The evaluation showed that patients understood the information objectively and subjectively. Consenting staff considered the broad consent model acceptable for the academic hospital setting and suggested improvements to increase the readability of information sheets and have more trained staff for better coordination. <b><i>Conclusion:</i></b> The IC development process and model consent are ethically sufficient, acceptable and feasible to be implemented in academic hospital-based biobanks in Indonesia adjusted to the business processes.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142010002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Ethanolic Extract of <i>Prosopis Farcta</i> and Trehalose on Cryopreserved Goat Epididymal Spermatozoa.","authors":"Saadoon Homar Ali, Abbas Farshad, Asaad Vaziry, Jalal Rostamzadeh, Farshad Ariyan","doi":"10.1089/bio.2023.0132","DOIUrl":"https://doi.org/10.1089/bio.2023.0132","url":null,"abstract":"<p><p>The purpose of this study, carried out in two experiments, was to investigate the antioxidant effect of <i>Prosopis farcta</i> in Experiment 1, and trehalose in Experiment 2 in the freezing extender, on the quality of frozen-thawed goat epididymal spermatozoa. Sperm samples were added to based egg-yolk Tris-extender containing experimental treatments. The first experimental treatments included the following: an extender of the control group without additive and extender containing 50, 100, or 150 µg/mL of <i>Prosopis farcta</i> ethanolic extract (PEE1, PEE2, and PEE3, respectively). Treatments of the second experiment include an extender of the control group without additive, an extender containing 100 mM of trehalose (Tr), an extender containing 100 µg/mL PEE2, and 100 µg/mL PEE2 + 100 mM Tr. The results of the first experiment showed that PEE2 compared with the control group led to a significant decrease (<i>p</i> < 0.05) in malondialdehyde (MDA) concentration. Also, PEE1 and PEE2 treatments resulted in a significant increase (<i>p</i> < 0.05) in motility parameters by computer-assisted sperm analysis, and MDA concentrations decreased significantly (<i>p</i> < 0.05) in all treatments compared with the control group. In general, the results of the present experiment showed that <i>Prosopis farcta</i> ethanolic extract at the level of 100 µg/mL was effective in improving the quality of frozen-thawed goat epididymal spermatozoa. Also, a combination of <i>Prosopis farcta</i> ethanolic extract and trehalose can be successful in freezing goat epididymal spermatozoa.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142010003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standard PREanalytical Code Version 4.0.","authors":"Fay Betsou, Rodrigo Chuaqui, Annemieke De-Wilde, William Grizzle, Michael Kiehntopf, Loes Linsen, William Mathieson, 'Ioana Moldovan, Rocío Aguilar-Quesada, Katheryn Shea, Purnima Singh, Stella Somiari, Demetri Spyropoulos, Juhi Tayal, Riad Tebbakha, Klara Valyi-Nagy, Lalita Wadhwa","doi":"10.1089/bio.2024.0010","DOIUrl":"https://doi.org/10.1089/bio.2024.0010","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141972366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}