{"title":"Equex-STM Paste-Added Freezing Medium and Straw Volume Influence Hampshire Crossbred Boar Semen Quality Following Cryopreservation.","authors":"Akash Protim Gogoi, Dipak Kumar Sarma, Himsikha Chakravarty, Sayed N Abedin, Gautam Khargharia, Rahul Katiyar, Kutubuddin Ahmed, Dhrubajyoti Borpujari, Arup Das, Champak Barman, Mahak Singh, Sourabh Deori","doi":"10.1089/bio.2024.0134","DOIUrl":"https://doi.org/10.1089/bio.2024.0134","url":null,"abstract":"<p><p>Cryopreservation of boar semen is essential for maintaining genetic diversity and improving reproductive efficiency. However, optimizing semen extenders and packaging methods is crucial to enhancing sperm quality and cryotolerance. Equex-STM is commonly used as a surfactant to enhance cryoprotective properties by stabilizing sperm membranes during freezing and thawing. A total of 24 ejaculates (<i>n</i> = 24), six from each of four Hampshire crossbred boars, aged between 18 and 24 months were used in the present study. Semen was collected twice weekly by gloved hand technique. The sperm-rich fraction showing more than 70% initial motility was considered for further processing and freezing. The fresh semen was diluted in Beltsville thawing solution (BTS) at a 1:1 ratio and further processed with the addition of Fraction I and Fraction II extenders supplemented with and without 1.5% Equex-STM paste {Control (C)-no Equex; Treatment (T-1.5% Equex-STM supplementation}. The extended semen was packaged in 0.25 and 0.5 mL straws to check the effect of straw size (T<sub>1</sub>-0.25 mL straw and T<sub>2</sub>-0.50 mL straw). Straws were cryopreserved in liquid nitrogen (LN<sub>2</sub>) for post-thaw sperm quality evaluation. Results showed that the sperm motility, viability, plasma membrane integrity (PMI), DNA integrity, and mitochondrial membrane potential (MMP) were significantly (<i>p</i> < 0.001) higher in the T group in comparison with control (C). Sperm motility, PMI, and DNA integrity were highly significant (<i>p</i> < 0.001) and sperm viability and MMP were significant (<i>p</i> < 0.05) in group T<sub>2</sub> in comparison with T<sub>1</sub>.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biobanking: Possibilities for Wastewater-Based Epidemiology.","authors":"Masaaki Kitajima, Hirohisa Abe, Ryo Honda, Hiroyuki Kobayashi, Tomohiro Kuroita, Ayuko Nemoto, Ryo Shirakashi, Rodney Scott, Koh Furuta","doi":"10.1089/bio.2024.0118","DOIUrl":"https://doi.org/10.1089/bio.2024.0118","url":null,"abstract":"<p><p>The COVID-19 pandemic, spanning from early 2020 to late 2022, posed unprecedented challenges for global public health. However, it also spurred innovative approaches to pandemic management, notably the development of pathogen detection in wastewater. It was successfully demonstrated that wastewater analysis can not only reflect ongoing COVID-19 infections but also serve as an early indicator of disease prevalence within communities. Recognizing the value of longitudinal analyses of various pathogens, we identified the need for wastewater biobanking. This practice allows for the retrospective analysis of samples, offering critical public health insights at the population level. Moreover, the potential to transport and store biobanked samples at ambient temperature or in a dry state could greatly enhance the utility of this technology, especially in resource-limited settings such as low- and middle-income countries. This article also addresses the ethical considerations and public health implications of wastewater-based epidemiology. While this approach holds significant potential beyond pathogen detection, it is essential to evaluate the benefits and potential risks carefully.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Evolution of Conservation Biobanking: A Literature Review and Analysis of Terminology, Taxa, Location, and Strategy of Wildlife Biobanks Over Time.","authors":"Devin M Chen, Gabriela F Mastromonaco","doi":"10.1089/bio.2024.0151","DOIUrl":"https://doi.org/10.1089/bio.2024.0151","url":null,"abstract":"<p><p>Nearly one-third of flora, fauna, and funga species on Earth are threatened with extinction. In response, the prevalence of repositories-often called \"biobanks\" or \"genome resource banks\"-for storing biological materials from threatened species has become more widespread. This research examined trends for the (1) terminology, (2) taxa representation, (3) global distribution, and (4) operational approach of biobanks versus genome resource banks relating to zoos and wildlife. Our literature search results indicate that although genome resource banking literature began earlier in the 1990s, biobanking has seen a surge in publications with over 3.5× more literature for biobanking since 2020. Genome resource bank articles were highly focused on mammals (68%), while biobanking literature focused more on multi-taxonomic overviews and less-studied taxa. Our search parameters found the largest number of wildlife biobanks in Europe (18) and the lowest number in South America (2), though results are likely impacted by the search being completed in English. Additionally, only 28% (7/25) of global biodiversity hotspots contain a wildlife biobank based on our methodology. While not all wildlife biobanking efforts are published or reported, these findings suggest that (1) \"biobank\" will likely be the more widely used term in the future, (2) more biobanking research is needed for non-mammalian taxa, (3) there are geographical gaps in wildlife biobanks, and (4) conservation biobanking programs should focus on storing biospecimens from a wide set of individuals and develop assisted reproductive technologies concomitantly with the goal of maintaining healthy, sustainable populations in the long term.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sandro Casavilca-Zambrano, Juan Contreras-Mancilla, Dany Córdova, Alicia Colombo, Jorge Honles, María Teresa García de Dávila, Milagros Abad-Licham, María López, Tatiana Vidaurre, Duniska Tarco, Gustavo Sarria, Francisco Berrospi, Elodie Caboux, Zisis Kozlakidis, Stéphane Bertani
{"title":"Advancing Cancer Research Capability in Peru Through the Integration of Registries With Biobanks.","authors":"Sandro Casavilca-Zambrano, Juan Contreras-Mancilla, Dany Córdova, Alicia Colombo, Jorge Honles, María Teresa García de Dávila, Milagros Abad-Licham, María López, Tatiana Vidaurre, Duniska Tarco, Gustavo Sarria, Francisco Berrospi, Elodie Caboux, Zisis Kozlakidis, Stéphane Bertani","doi":"10.1089/bio.2024.0158","DOIUrl":"10.1089/bio.2024.0158","url":null,"abstract":"<p><p>This article highlights Peru's experience in establishing a national tumor bank network, serving as a model for low- and middle-income countries. Launched in 2005 at the National Institute of Neoplastic Diseases, efforts accelerated under the 2021 National Cancer Act, which formalized the National Tumor Bank and its integration with the National Oncology Network. This initiative connects tumor banks across regional cancer institutes, enabling systematic biological sample collection, particularly from underrepresented populations, such as those with high Amerindian ancestry. Ethical oversight, technical standards, and specialized management software ensure efficient data sharing and genomic research. The network supports cancer research through integration with the Population Cancer Registry, providing unique insights into cancer incidence and outcomes. To date, 5992 cases have been documented. Through international collaboration with Latin American countries, Peru provides a framework for inclusive cancer research, enriching global genomic datasets and strengthening research capacity in diverse and vulnerable populations.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Curcumin Protects Human Sperm Quality During Cryopreservation.","authors":"Banafsheh Mansouri-Bahrani, Maryam Azizi Kutenaee, Mitra Heydari Nasrabadi, Ensieh Salehi","doi":"10.1089/bio.2023.0091","DOIUrl":"https://doi.org/10.1089/bio.2023.0091","url":null,"abstract":"<p><p><b><i>Background:</i></b> Cryopreservation causes harmful effects on sperm quality due to reactive oxygen species (ROS) overproduction and physical-chemical modifications, resulting in reduced sperm fertility potential. Recently, many studies have shown that adding antioxidants to the cryopreservation medium can markedly reduce these damages. The present study aimed to evaluate the effects of pre-treatment with curcumin at 0, 20, 50, and 100 μM concentrations on frozen-thawed human sperm parameters. <b><i>Methods:</i></b> Semen samples from 25 normozoospermic men were collected. Then, each sample was divided into five equal parts: fresh group and frozen-thawed groups, including 0, 20, 50, and 100 μM of curcumin. Pre-cryopreservation and post-thaw sperm motility, morphology, vitality, DNA fragmentation, and ROS levels were investigated. <b><i>Results:</i></b> Cryopreservation significantly reduced sperm quality. A known value of 50 μM curcumin significantly improved sperm progressive motility (18.67 ± 1.12 vs. 11.2 ± 1.24, <i>p</i> < 0.01), vitality (35.50 ± 1.63 vs. 21.83 ± 2.64, <i>p</i> < 0.05), and decreased ROS levels (<i>p</i> < 0.05), 50 μM curcumin also efficiently preserved sperm morphology after thawing (13.55 ± 0.33 vs. 6.56 ± 0.16, <i>p</i> < 0.001). Furthermore, the application of 50 μM curcumin resulted in a reduction in DNA fragmentation, though it did not reach statistical significance (<i>p</i> = 0.08). In contrast, 20 μM curcumin only had a significant impact on progressive motility (15.85 ± 0.7 vs. 11.2 ± 1.24, <i>p</i> < 0.05), whereas, in the 100 μM group, there were no significant differences in any of the measured parameters compared with the control group. <b><i>Conclusion:</i></b> It seems that curcumin ameliorates cryopreservation-induced injury to sperm.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Conceptual Uncertainties and Practical Challenges in Voluntary Nagoya Protocol Compliance: The Australian Situation.","authors":"Hamish MacDonald","doi":"10.1089/bio.2024.0090","DOIUrl":"https://doi.org/10.1089/bio.2024.0090","url":null,"abstract":"<p><p>Amidst growing international pressure for institutions that collect biological material to comply with the Nagoya Protocol, scientific gatekeepers such as herbaria, funding bodies, and academic journals increasingly request proof of Nagoya Protocol compliance. What happens when research is conducted in a country which does not have a comprehensive regulatory framework implementing the Nagoya Protocol? This article addresses this question through an examination of the difficulties that genetic resource collectors and biobankers may encounter in attempting to voluntarily comply with the Nagoya Protocol in Australia, a country that has not ratified the Nagoya Protocol at a federal level. It summarizes the requirements of the Nagoya Protocol, surveys the legal and regulatory situation that currently exists in Australia, and outlines the difficulties and ambiguities encountered by scientists and biobankers in attempting to navigate this system. In the process, it provides an overview of the conceptual and linguistic ambiguities which exist within the framework of the Nagoya Protocol. It argues that consensus models such as voluntary guidelines may be useful for addressing some of these ambiguities and practical challenges, but more fundamental issues will likely require legislative intervention.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":"23 1","pages":"23-30"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Acknowledgment of Reviewers 2024.","authors":"","doi":"10.1089/bio.2024.31587.revack","DOIUrl":"https://doi.org/10.1089/bio.2024.31587.revack","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":"23 1","pages":"67-68"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alicia Sweeney, Amanda Rush, Julia Stevens, Greg T Sutherland
{"title":"A Survey of Community Perceptions on Brain Donation for Research.","authors":"Alicia Sweeney, Amanda Rush, Julia Stevens, Greg T Sutherland","doi":"10.1089/bio.2023.0158","DOIUrl":"10.1089/bio.2023.0158","url":null,"abstract":"<p><p>Postmortem brain donation for medical research is a little-known form of organ donation. While most brain research is carried out using animal models, many neurological diseases are uniquely human. Greater availability of human postmortem brain tissue from diseased individuals and controls would therefore improve the development of treatments for neurological and neuropsychiatric diseases. Globally, organ donation for medical research is dwarfed by organ donation for transplantation. In 2021, 36% of Australians were registered organ donors for transplantation, with public \"in-principle\" support even higher, at 76%. In contrast, there are little data on Australian or international brain donation rates for research. A 30-item online survey was conducted to ascertain knowledge of, and attitudes toward, brain donation in Australia. Of the respondents, 12/237 (5%) were current brain donors and excluded from further analysis. Of the remaining 225, 75% were registered organ donors for transplant. The vast majority (<i>n</i> = 189/225, 84%) of respondents supported or strongly supported the principle of brain donation. However, of those registered for transplantation or whole-body donors, 93/170 (55%) were not aware that brain donation was possible, while 50%, alternatively or also, thought that registering as an organ donor for transplantation rendered them a brain donor by default. Only 9/225 (4%) respondents indicated that they would definitely not donate their brain in the future, while 27 remained unsure. There is prominent public support for brain donation in Australia, with 84% of respondents willing to donate their brain. Yet, the extent of public misconceptions on brain donation for research suggests the need for further education on all types of organ donation, so individuals may make informed decisions.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"31-38"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140066277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"MIBLood-EV: An Online Reporting Tool to Facilitate the Standardized Reporting of Preanalytical Variables and Quality Control of Plasma and Serum to Enhance Rigor and Reproducibility in Liquid Biopsy Research.","authors":"Dakota Gustafson, Rienk Nieuwland, Fabrice Lucien","doi":"10.1089/bio.2024.0083","DOIUrl":"10.1089/bio.2024.0083","url":null,"abstract":"<p><p>Pre-analytical variability significantly impacts the reproducibility of liquid biopsy research, which is critical for precision medicine and biomedical research. This report highlights the challenges and variability in the pre-analytical processes of liquid biopsies, especially regarding extracellular vesicles (EVs), which are crucial for diagnostics in oncology. The MIBlood-EV initiative aims to standardize the reporting of pre-analytical variables and the quality control of plasma and serum samples to enhance reproducibility in EV research. By providing a comprehensive and flexible reporting framework, MIBlood-EV seeks to improve the reliability of EV studies and facilitate the development of evidence-based protocols, ultimately advancing the field of liquid biopsy research.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"62-64"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142156678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tanmaya Atre, Vi Nguyen, Veronica Chow, Gregor S D Reid, Suzanne Vercauteren
{"title":"A Comparative Study of B Cell Blast Isolation Methods from Bone Marrow Aspirates of Pediatric Leukemia Patients.","authors":"Tanmaya Atre, Vi Nguyen, Veronica Chow, Gregor S D Reid, Suzanne Vercauteren","doi":"10.1089/bio.2023.0133","DOIUrl":"10.1089/bio.2023.0133","url":null,"abstract":"<p><p>Density gradient centrifugation is a conventional technique widely utilized to isolate bone marrow mononuclear cells (BM-MNC) from bone marrow (BM) aspirates obtained from pediatric B-cell acute lymphoblastic leukemia (B-ALL) patients. Nevertheless, this technique achieves incomplete recovery of mononuclear cells and is relatively time-consuming and expensive. Given that B-ALL is the most common childhood malignancy, alternative methods for processing B-ALL samples may be more cost-effective. In this pilot study, we use several readouts, including immune phenotype, cell viability, and leukemia-initiating capacity in immune-deficient mice, to directly compare the density gradient centrifugation and buffy coat processing methods. Our findings indicate that buffy coat isolation yields comparable BM-MNC product in terms of both immune and leukemia cell content and could provide a viable, lower cost alternative for biobanks processing pediatric leukemia samples.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"46-52"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}