Association of Serum Metabolites with Serum Indices and Preanalytical Factors of Biobanked Serum Samples.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-08 DOI:10.1089/bio.2023.0130

Juyoung Lee, Byeong-Gon Ji, Eun-Jung Hong, Jae-Pil Jeon

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引用次数: 0

Abstract

Background: Serum indices (hemolysis, icterus, and lipemia; HIL) are known to impact clinical chemistry assay results. This study aimed to investigate the impact of HIL indices on serum metabolite profiles and the association of serum metabolite levels with pre-analytical factors of serum samples. Methods: A cohort of serum samples (n = 12,196) from the Korean Genome and Epidemiology Study (KoGES) was analyzed for HIL indices and the pre-analytical variables (SPRECs) which were generated in the process of serum collection. We further performed targeted metabolomics on a subset comprising hemolyzed (n = 60), icteric (n = 60), lipemic (n = 60) groups, and a common control group of non-HIL samples (n = 60) using the Absolute IDQ p180 kit. Results: We found 22 clinical chemistry analytes significantly associated with hemolysis, 25 with icterus, and 24 with lipemia (p < 0.0001). Serum metabolites (n = 27) were associated with all of hemolysis, icterus, and lipemia (p < 0.05). The PC ae C36 2 had exhibited a significant association with pre-analytical factors corresponding to the third (pre-centrifugation delay between processing) and sixth (post-centrifugation) elements of the SPREC. Conclusions: This study showed the association of the serum index and pre-analytical factors with serum metabolite profiles. In addition, the association of pre-analytical factors with serum metabolite concentrations would corroborate the utility of SPRECs for the quality control of biobanked serum samples.

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血清代谢物与生物库血清样本的血清指标和分析前因素的关系

背景：众所周知，血清指数（溶血、黄疸和脂血；HIL）会影响临床化学分析结果。本研究旨在探讨 HIL 指数对血清代谢物谱的影响，以及血清代谢物水平与血清样本分析前因素的关联。研究方法我们对韩国基因组与流行病学研究（KoGES）的一组血清样本（n = 12,196）进行了HIL指数和血清采集过程中产生的分析前变量（SPRECs）分析。我们还使用 Absolute IDQ p180 试剂盒对溶血组（n = 60）、黄疸组（n = 60）、脂血组（n = 60）和非 HIL 样品的普通对照组（n = 60）进行了靶向代谢组学分析。结果我们发现有 22 种临床生化分析物与溶血、25 种与黄疸、24 种与脂血明显相关（P < 0.0001）。血清代谢物（n = 27）与所有溶血、黄疸和脂血相关（p < 0.05）。PC ae C36 2与分析前因素有显著关联，这些因素分别对应于SPREC的第三要素（离心前处理延迟）和第六要素（离心后）。结论本研究显示了血清指数和分析前因素与血清代谢物谱的关联。此外，分析前因素与血清代谢物浓度的关联也证实了 SPRECs 在生物库血清样本质量控制中的实用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.