Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"Primary amino acid sequences of decapod (Na+, K+)-ATPase provide evolutionary insights into osmoregulatory mechanisms","authors":"Leonardo M. Fabri ,&nbsp;Cintya M. Moraes ,&nbsp;Daniela P. Garçon ,&nbsp;John C. McNamara ,&nbsp;Samuel C. Faria ,&nbsp;Francisco A. Leone","doi":"10.1016/j.cbpa.2024.111696","DOIUrl":"10.1016/j.cbpa.2024.111696","url":null,"abstract":"<div><p>Decapod Crustacea exhibit a marine origin, but many taxa have occupied environments ranging from brackish to fresh water and terrestrial habitats, overcoming their inherent osmotic challenges. Osmotic and ionic regulation is achieved by the gill epithelia, driven by two active ATP-hydrolyzing ion transporters, the basal (Na⁺, K⁺)-ATPase and the apical V(H⁺)-ATPase. The kinetic characteristic of gill (Na⁺, K⁺)-ATPase and the mRNA expression of its α subunit have been widely studied in various decapod species under different salinity challenges. However, the evolution of the primary structure has not been explored, especially considering the functional modifications associated with decapod phylogeny. Here, we proposed a model for the topology of the decapod α subunit, identifying the sites and motifs involved in its function and regulation, as well as the patterns of its evolution assuming a decapod phylogeny. We also examined both the amino acid substitutions and their functional implications within the context of biochemical and physiological adaptation. The α-subunit of decapod crustaceans shows greater conservation (∼94% identity) compared to the β-subunit (∼40%). While the binding sites for ATP and modulators are conserved in the decapod enzyme, the residues involved in the α-β interaction are only partially conserved. In the phylogenetic context of the complete sequence of (Na⁺, K⁺)-ATPase α-subunit, most substitutions appear to be characteristic of the entire group, with specific changes for different subgroups, especially among brachyuran crabs. Interestingly, there was no consistent separation of α-subunit partial sequences related to habitat, suggesting that the convergent evolution for freshwater or terrestrial modes of life is not correlated with similar changes in the enzyme's primary amino acid sequence.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Common methodological issues in comparative biochemistry and physiology","authors":"Chris N. Glover ,&nbsp;Brittney G. Borowiec ,&nbsp;William Joyce","doi":"10.1016/j.cbpa.2024.111697","DOIUrl":"10.1016/j.cbpa.2024.111697","url":null,"abstract":"","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species-specific differences and temperature-dependence of Na+/K+-ATPase in freshwater mussels Anodonta anatina and Unio tumidus (Bivalvia: Unionidae)","authors":"","doi":"10.1016/j.cbpa.2024.111698","DOIUrl":"10.1016/j.cbpa.2024.111698","url":null,"abstract":"<div><p>The predicted global warming of surface waters can be challenging to aquatic ectotherms like freshwater mussels. Especially animals in northern temperate latitudes may face and physiologically acclimate to significant stress from seasonal temperature fluctuations. Na⁺/K⁺-ATPase enzyme is one of the key mechanisms that allow mussels to cope with changing water temperatures. This enzyme plays a major role in osmoregulation, energy control, ion balance, metabolite transport and electrical excitability. Here, we experimentally studied the effects of temperature on Na⁺/K⁺-ATPase activity of gills in two freshwater mussel species, <em>Anodonta anatina</em> and <em>Unio tumidus</em>. The study animals were acclimated to three ambient temperatures (+4, +14, +24 °C) and Na⁺/K⁺-ATPase activity was measured at those temperatures for each acclimation group. Both species had their highest gill Na⁺/K⁺-ATPase activity at the highest acclimation temperature. Na⁺/K⁺-ATPase activity of gills exhibited species-specific differences, and was higher in <em>A. anatina</em> than <em>U. tumidus</em> in all test groups at all test temperatures. Temperature dependence of Na⁺/K⁺-ATPase was confirmed in both species, being highest at temperatures between +4 and + 14 °C when <em>Q</em>₁₀ values in the acclimation groups varied between 5.06 and 6.71. Our results underline the importance of Na⁺/K⁺-ATPase of gills for the freshwater mussels in warming waters. Because Na⁺/K⁺-ATPase is the driving force behind ciliary motion, our results also suggest that in warming waters <em>A. anatina</em> may be more tolerant at sustaining vigorous ciliary action (associated with elevated respiration rates and filter-feeding) than <em>U. tumidus</em>. Overall, our results indicate great flexibility of the mussel's ecophysiological characteristics as response to changing conditions.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1095643324001259/pdfft?md5=e7ef855a699ca2dc75d62de74fdd52e2&pid=1-s2.0-S1095643324001259-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141602207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light sensitivity in Beroidae ctenophores: Insights from laboratory studies and genomics","authors":"Olga V. Krivenko,&nbsp;Olga N. Kuleshova,&nbsp;Iuliia S. Baiandina","doi":"10.1016/j.cbpa.2024.111694","DOIUrl":"10.1016/j.cbpa.2024.111694","url":null,"abstract":"<div><p>Light detection underlies a variety of animal behaviors, including those related to spatial orientation, feeding, avoidance of predators, and reproduction. Ctenophores are likely the oldest animal group in which light sensitivity based on opsins evolved, so they may still have the ancestral molecular mechanisms for photoreception. However, knowledge about ctenophore photosensitivity, associated morphological structures, molecular mechanisms involved, and behavioral reactions is limited and fragmented. We present the initial experiments on the responses of adult <em>Beroe ovata</em> to high-intensity light exposure with different spectra and photosensitivity in various parts of the animal's body. Ctenophores have shown a consistent behavioral response when their aboral organ is exposed to a household-grade laser in the violet spectrum. To investigate the genes responsible for the photosensitivity of Beroidae, we have analyzed transcriptome and genome-wide datasets. We identified three opsins in <em>Beroe</em> that are homologous to those found in <em>Mnemiopsis leidyi</em> (Lobata) and <em>Pleurobrachia bachei</em> (Cydippida). These opsins form clades Ctenopsin1, 2, and 3, respectively. Ctenopsin3 is significantly distinct from other ctenophore opsins and clustered outside the main animal opsin groups. The Ctenopsin1 and Ctenopsin2 groups are sister clusters within the canonical animal opsin tree. These two groups could have originated from gene duplication in the common ancestor of the species we studied and then developed independently in different lineages of Ctenophores. So far, there is no evidence of additional expansion of the opsin family in ctenophore evolution. The involvement of ctenophore opsins in photoreception is discussed by analyzing their protein structures.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological responses of Holothuria grisea during a wound healing event: An integrated approach combining tissue, cellular and humoral evidence","authors":"Patrícia Lacouth ,&nbsp;Alessandra Majer ,&nbsp;Vincenzo Arizza ,&nbsp;Mirella Vazzana ,&nbsp;Manuela Mauro ,&nbsp;Márcio Reis Custódio ,&nbsp;Vinicius Queiroz","doi":"10.1016/j.cbpa.2024.111695","DOIUrl":"10.1016/j.cbpa.2024.111695","url":null,"abstract":"<div><p>Due to their tissue structure similar to mammalian skin and their close evolutionary relationship with chordates, holothurians (Echinodermata: Holothuroidea) are particularly interesting for studies on wound healing. However, previous studies dealing with holothuroid wound healing have had limited approaches, being restricted to tissue repair or perivisceral immune response. In this study, we combined tissue, cellular and humoral parameters to study the wound healing process of <em>Holothuria grisea</em>. The immune responses of the perivisceral coelom were assessed by analyzing the number, proportion and viability of coelomocytes and the volume and protein concentration of the coelomic fluid. Additionally, the morphology of the healing tissue and number of coelomocytes in the connective tissue of different body wall layers were examined over 30 days. Our results showed that perivisceral reactions started 3 h after injury and decreased to baseline levels within 24 h. In contrast, tissue responses were delayed, beginning after 12 h and returning to baseline levels only after day 10. The number of coelomocytes in the connective tissue suggests a potential cooperation between these cells during wound healing: phagocytes and acidophilic spherulocytes act together in tissue clearance/homeostasis, whereas fibroblast-like and morula cells cooperate in tissue remodeling. Finally, our results indicate that the major phases observed in mammalian wound healing are also observed in <em>H. grisea</em>, despite occurring at a different timing, which might provide insights for future studies. Based on these data, we propose a model that explains the entire healing process in <em>H. grisea</em>.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of ETH receptor and its possible roles in the mud crab Scylla paramamosain","authors":"","doi":"10.1016/j.cbpa.2024.111692","DOIUrl":"10.1016/j.cbpa.2024.111692","url":null,"abstract":"<div><p>Ecdysis-triggering hormone (ETH) is a neuropeptide hormone characterized by a conserved KxxKxxPRx amide structure widely identified in arthropods. While its involvement in the regulation of molting and reproduction in insects is well-established, its role in crustaceans has been overlooked. This study aimed to de-orphanise a receptor for ETH in the mud crab <em>Scylla paramamosain</em> and explore its potential impact on ovarian development. A 513-amino-acid G protein-coupled receptor for ETH (<em>Sp</em>ETHR) was identified in <em>S. paramamosain</em>, exhibiting a dose-dependent activation by <em>Sp</em>ETH with an EC₅₀ value of 75.18 nM. Tissue distribution analysis revealed <em>SpETH</em> was in the cerebral ganglion and thoracic ganglion, while <em>SpETHR</em> was specifically expressed in the ovary, hepatopancreas, and Y-organ of female crabs. <em>In vitro</em> experiments demonstrated that synthetic <em>Sp</em>ETH (at a concentration of 10⁻⁸ M) significantly increased the expression of <em>SpVgR</em> in the ovary and induced ecdysone biosynthesis in the Y-organ. <em>In vivo</em> experiments showed a significant upregulation of <em>SpEcR</em> in the ovary and <em>Disembodied</em> and <em>Shadow</em> in the Y-organ after 12 h of <em>Sp</em>ETH injection. Furthermore, a 16-day administration of <em>Sp</em>ETH significantly increased 20E titers in hemolymph, gonadosomatic index (GSI) and oocyte size of <em>S. paramamosain.</em> In conclusion, our findings suggest that <em>Sp</em>ETH may play stimulatory roles in ovarian development and ecdysone biosynthesis by the Y-organ.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141560424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short communication: Maintained visual performance in birds under high altitude hypoxia","authors":"Naja Kirstine Christensen,&nbsp;Kristian Beedholm,&nbsp;Christian Damsgaard","doi":"10.1016/j.cbpa.2024.111691","DOIUrl":"10.1016/j.cbpa.2024.111691","url":null,"abstract":"<div><p>Birds are highly dependent on their vision for orientation and navigation. The avian eye differs from the mammalian eye as the retina is avascular, leaving the inner, highly metabolically active layers with a very long diffusion distance to the oxygen supply. During flight at high altitudes, birds face a decrease in environmental oxygen partial pressure, which leads to a decrease in arterial oxygen levels. Since oxygen perfusion to the retina is already limited in birds, we hypothesize that visual function is impaired by low oxygen availability. However, the visual performance of birds exposed to hypoxia has not been evaluated before. Here, we assess the optomotor response (OMR) in zebra finches under simulated high-altitude hypoxia (10%) and show that the OMR is largely maintained under hypoxia with only a modest reduction in OMR, demonstrating that birds can largely maintain visual function at high altitudes. The method of our study does not provide insight into the mechanisms involved, but our findings suggest that birds have evolved physiological mechanisms for retinal function at low tissue oxygen levels.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1095643324001181/pdfft?md5=f1fa15f918051042de465a2b429eff13&pid=1-s2.0-S1095643324001181-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does the activation of sea bass GnIH receptor modulate GnRH receptor signaling?","authors":"Bin Wang ,&nbsp;José A. Paullada-Salmerón ,&nbsp;Alba Vergès-Castillo ,&nbsp;José A. Muñoz-Cueto","doi":"10.1016/j.cbpa.2024.111693","DOIUrl":"10.1016/j.cbpa.2024.111693","url":null,"abstract":"<div><p>Previous studies have revealed the stimulatory and inhibitory actions of gonadotropin-releasing hormone (GnRH) and gonadotropin-inhibitory hormone (GnIH) on the control of reproduction in European sea bass (<em>Dicentrarchus labrax</em>) and other vertebrates, respectively. However, information on the possible interactions between GnRH and GnIH on cell signaling is sparse in vertebrates. In the current study, we investigated if activation of sea bass GnIH receptor (GnIHR) can interfere with GnRH receptor II-1a (GnRHR-II-1a) involving the PKA pathway. Our results showed that GnIH and GnRH functioned via their cognate receptors, respectively. However, it appears that neither GnIH1 nor GnIH2 can block GnRH/GnRHR-II-1a-induced PKA signaling in sea bass. This is the first study to examine the potential interactions of GnIH with GnRH receptor signaling in teleosts. Further research seems necessary to shed light on unknown interactions in other signaling pathways and other GnIH/GnRH receptors involved in the physiological functions of these two relevant neuropeptides, not only in sea bass but also in other species.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S109564332400120X/pdfft?md5=784c934882cfecbecd7c0aefb0761a01&pid=1-s2.0-S109564332400120X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141538972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commentary: The microbial dependence continuum: Towards a comparative physiology approach to understand host reliance on microbes","authors":"Claire E. Williams ,&nbsp;Samantha S. Fontaine","doi":"10.1016/j.cbpa.2024.111690","DOIUrl":"10.1016/j.cbpa.2024.111690","url":null,"abstract":"<div><p>Comparative physiologists often compare physiological traits across organisms to understand the selective pressures influencing their evolution in different environments. Traditionally focused on the organisms themselves, comparative physiology has more recently incorporated studies of the microbiome—the communities of microbes living in and on animals that influence host physiology. In this commentary, we describe the utility of applying a comparative framework to study the microbiome, particularly in understanding how hosts vary in their dependence on microbial communities for physiological function, a concept we term the “microbial dependence continuum”. This hypothesis suggests that hosts exist on a spectrum ranging from high to low reliance on their microbiota. Certain physiological traits may be highly dependent on microbes for proper function in some species but microbially independent in others. Comparative physiology can elucidate the selective pressures driving species along this continuum. Here, we discuss the microbial dependence continuum in detail and how comparative physiology can be useful to study it. Then, we discuss two example traits, herbivory and flight, where comparative physiology has helped reveal the selective pressures influencing host dependence on microbial communities. Lastly, we discuss useful experimental approaches for studying the microbial dependence continuum in a comparative physiology context.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of red-light irradiation and melatonininjection on the antioxidant capacity and occurrence of apoptosis in abalones (Haliotis discus hannai) subjected to thermal stress","authors":"Young Hoon Kwon ,&nbsp;Jin A Kim ,&nbsp;Young-Su Park ,&nbsp;Jun-Hwan Kim ,&nbsp;Cheol Young Choi","doi":"10.1016/j.cbpa.2024.111689","DOIUrl":"10.1016/j.cbpa.2024.111689","url":null,"abstract":"<div><p>High ocean temperatures caused by global warming induce oxidative stress in aquatic organisms. Melatonin treatment and irradiation using red light-emitting diodes (LEDs) have been reported to reduce oxidative stress in a few aquatic organisms. However, the effects of red LED irradiation and melatonin injection on the antioxidant capacity and degree of apoptosis in abalones, which are nocturnal organisms, have not yet been reported. In this study, we compared the expression levels of antioxidant enzymes, total antioxidant capacity, and the degree of apoptosis in abalones subjected to red LED irradiation and melatonin treatment. The results revealed that at high water temperatures (25 °C), the mRNA expression levels of the superoxide dismutase (SOD) and glutathione peroxidase (GPx) genes and the antioxidant activity of SOD decreased in abalones in the red-LED irradiated and melatonin-treated groups compared with those in abalones in the control group. Although high water temperatures induced DNA damage in the abalone samples, the degree of apoptosis was lower in the red-LED irradiated and melatonin-treated groups than in the control group. Overall, the abalones in the melatonin-treated and red-LED irradiated groups showed reduced oxidative stress and increased antioxidant enzyme levels under thermal stress compared with those in the control group. Therefore, red LED irradiation is a promising alternative to melatonin treatment, which is difficult to administer continuously for a long time, for protecting abalones from oxidative stress.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}