Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"Bioimaging and the future of whole-organismal developmental physiology","authors":"Oliver Tills,&nbsp;Ziad Ibbini,&nbsp;John I. Spicer","doi":"10.1016/j.cbpa.2024.111783","DOIUrl":"10.1016/j.cbpa.2024.111783","url":null,"abstract":"<div><div>While omics has transformed the study of biology, concomitant advances made at the level of the whole organism, <em>i.e.</em> the phenome, have arguably not kept pace with lower levels of biological organisation. In this personal commentary we evaluate the importance of imaging as a means of measuring whole organismal developmental physiology. Image acquisition, while an important process itself, has become secondary to image analysis as a bottleneck to the use of imaging in research. Here, we explore the significant potential for increasingly sophisticated approaches to image analysis, including deep learning, to advance our understanding of how developing animals grow and function. Furthermore, unlike many species-specific methodologies, tools and technologies, we explore how computer vision has the potential to be transferable between species, life stages, experiments and even taxa in which embryonic development can be imaged. We identify what we consider are six of the key challenges and opportunities in the application of computer vision to developmental physiology carried out in our lab, and more generally. We reflect on the tangibility of transferrable computer vision models capable of measuring the integrative physiology of a broad range of developing organisms, and thereby driving the adoption of phenomics for developmental physiology. We are at an exciting time of witnessing the move from computer vision as a replacement for manual observation, or manual image analysis, to it enabling a fundamentally more powerful approach to exploring and understanding the complex biology of developing organisms, the quantification of which has long posed a challenge to researchers.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"300 ","pages":"Article 111783"},"PeriodicalIF":2.1,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of temperature changes on oxidative stress and antioxidant defense system in the bay scallop, Argopecten irradians","authors":"Jin Ah Song ,&nbsp;Eunseong Lee ,&nbsp;Young-Ung Choi ,&nbsp;Jordan Jun Chul Park ,&nbsp;Jeonghoon Han","doi":"10.1016/j.cbpa.2024.111775","DOIUrl":"10.1016/j.cbpa.2024.111775","url":null,"abstract":"<div><div>In this study, we aimed to understand the effects of changes in temperature on biochemical and molecular responses associated with the antioxidant defense system in the bay scallop, <em>Argopecten irradians</em>. We measured the contents of H₂O₂ and malondialdehyde (MDA), as well as the activities of antioxidant enzymes (e.g., glutathione <em>S-</em>transferase [GST], superoxide dismutase [SOD], and catalase [CAT]), and the regulation of stress-related genes (e.g., <em>GST</em>, <em>SOD</em>, <em>CAT</em>, and heat shock protein 70 [<em>HSP70</em>]). In addition, total antioxidant capacity (TAC) was examined in scallops exposed to different temperatures. <em>A. irradians</em> showed high levels of H₂O₂ and MDA in response to acute thermal stress (48 and 72 h of exposure). Temperature changes also led to a significant increase in antioxidant enzyme activity and mRNA expression levels in <em>A. irradians</em>. Interestingly, the TAC increased in response to acute thermal stress (28 °C) for up to 12 h and decreased thereafter. The oxidative stress induced by high temperatures could not be alleviated by an increase in levels of antioxidant enzymes, such as GST, SOD, and CAT, resulting in high levels of H₂O₂ and MDA and low levels of TAC. In addition, significant changes (<em>P</em> &lt; 0.05) in <em>HSP70</em> levels were observed in response to changes in temperature, suggesting that HSP70 played an important role in the heat tolerance of <em>A. irradians</em>. In conclusion, <em>A. irradians</em> exhibits a greater degree of oxidative stress responses in high-temperature environments than that in low-temperature environments. Overall, these findings indicate that temperature changes lead to oxidative stress, resulting in cellular damage and activation of the antioxidant defense system in bay scallops. Further experiments are required to elucidate other antioxidants and fully understand the redox system in <em>A. irradians</em>.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111775"},"PeriodicalIF":2.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acute exposure to freshwater acidification on developing Oryzias latipes","authors":"Grace E. Wallace,&nbsp;Rosemary C. Minns,&nbsp;Caleb T. Hasler","doi":"10.1016/j.cbpa.2024.111774","DOIUrl":"10.1016/j.cbpa.2024.111774","url":null,"abstract":"<div><div>Elevated CO₂ in aquatic environments causes weak acidification. When exposed to weak acidification, regardless of life stage, most fishes undergo some degree of hypercapnia (elevation of CO₂ in the bloodstream). Hypercapnia negatively affects physiological processes and embryo and larval fish are particularly vulnerable to rises in CO₂. The aim of our study was to understand if weak acidification induced by elevated CO₂ alters the physiology and behaviour of freshwater Japanese medaka (<em>Oryzias latipes</em>) embryos and larvae. To test this, we treated Japanese medaka embryos and larvae for 24 h with varying levels of weak acidification (pH 7.1 [∼482 μatm], 6.4 [∼2,122 μatm], 6.1 [∼3,280 μatm], 5.8 [∼5,306 μatm], and 5.7 [∼10,130 μatm]) at two developmental stages (72 hpf and 9 dpf). Following the treatment, heart rate, burst activity (movement within the egg), and survival of embryos were quantified. Swimming activity of hatched larvae was also measured. We observed a statistically significant 2-fold decline in heart rate of embryonic Japanese medaka as pH decreased (<em>P</em> &lt; 0.01). We also found that survival of embryos significantly declined as acidification increased (<em>P</em> &lt; 0.01). Behaviour of larval fish was significantly altered (<em>P</em> &lt; 0.001) but not in a pH dependent manner. Our study suggests that weak acidification can cause negative effects to early life stage physiology and that behaviour can be altered. Our results suggest that if fish develop in weakly acidified freshwater there may be unfavourable impacts, including mortality at the most extreme levels.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"300 ","pages":"Article 111774"},"PeriodicalIF":2.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water fluxes and nutrient absorption along the midgut of three hemipterans, Mahanarva fimbriolata, Dysdercus peruvianus, and Rhodnius prolixus","authors":"Ignacio G. Barroso,&nbsp;Bárbara B. Nascimento,&nbsp;Clelia Ferreira,&nbsp;Walter R. Terra","doi":"10.1016/j.cbpa.2024.111773","DOIUrl":"10.1016/j.cbpa.2024.111773","url":null,"abstract":"<div><div>Hemiptera Order comprises insect species adapted to different diets regarding water and nutrient content and availability, thus suggesting different combinations of proteins to ensure their absorption. To find out whether hemipterans use the same or distinct set of proteins and whether these differences are related to the phylogeny or the diet, RNAseq analyses were conducted in gut sections of three hemipterans, <em>M. fimbriolata, D. peruvianus</em>, and <em>R. prolixus,</em> with remarkable distinct diet. Since only a few of the selected proteins were functionally characterized, the coded putative proteins were manually curated by bioinformatics to infer their physiological function. The results suggest a relationship between gene expression patterns and water and nutrient dietary content and availability. In contrast, putative gene expansions and deletions are related to phylogeny, corresponding to evolutionary adaptations of ancestral forms to feed on xylem, cotton seeds, and blood, resulting in more resemblances between <em>D. peruvianus</em> and <em>R. prolixus</em> than <em>M. fimbriolata. M. fimbriolata</em> absorbs water through aquaporins Drip and Prip in the filtration chamber by passive diffusion, with a higher contribution of water-selective Drip. <em>D. peruvianus</em> water absorption involves Drip and Prip, but Prip contribution appears to be higher, and they probably cooperate with water-ion cotransporters in the posterior midgut. <em>R. prolixus</em> absorbs water in the anterior midgut involving a sodium transporter and a putative water-urea Prip. Sugars, amino acids, and lipids might be absorbed along the midgut in the three species, with a higher contribution of the posterior midgut for amino acid and lipid absorption in <em>M. fimbriolata</em> and <em>D. peruvianus</em> and the middle midgut in <em>R. prolixus</em>.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111773"},"PeriodicalIF":2.1,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daily rhythms of locomotor activity and transcript levels of non-visual opsins in the brain of the blind Mexican cavefish (Astyanax mexicanus)","authors":"Francesca Conti ,&nbsp;Alba Vergès-Castillo ,&nbsp;Francisco J. Sánchez-Vázquez ,&nbsp;José F. López-Olmeda ,&nbsp;Cristiano Bertolucci ,&nbsp;José A. Muñoz-Cueto","doi":"10.1016/j.cbpa.2024.111772","DOIUrl":"10.1016/j.cbpa.2024.111772","url":null,"abstract":"<div><div>Most organisms possess endogenous circadian clocks that synchronise their physiology and behaviour with environmental cycles, with the light-dark (LD) cycle being the most potent synchronising signal. Consequently, it can be hypothesised that animals that have evolved in the dark, as in caves or deep sea, may no longer possess a functional light-entrained biological clock. In this research, the blind cavefish <em>Astyanax mexicanus</em> was selected as a model organism to investigate the potential effects of daily light conditions on the circadian timekeeping mechanisms. First, we focused on describing behavioural photic entrainment and the presence of a circadian endogenous rhythmicity by recording locomotor activity rhythms under different lighting regimes: LD 12:12, after a 6-h shift of LD, constant darkness (DD), and constant dim light (LLdim). Secondly, we aimed at characterising the mechanisms of photodetection by analysing the daily rhythms of expression of selected non-visual extraocular opsins (<em>exo-rhod</em>, <em>opn3</em>, <em>rgra</em>, <em>rgrb</em>, <em>tmt1a</em> and <em>tmt1b</em>) in the brain of this blind species using real-time quantitative PCR. Our results revealed that blind Mexican cavefish activity rhythms were entrained to the LD cycle, with a diurnal activity pattern that persisted in a circadian fashion under constant lighting conditions. Additionally, statistically significant daily variations and/or rhythms were observed in three out of the six non-visual opsin genes analysed (<em>opn3</em>, <em>rgra</em> and <em>tmt1b</em>), all of them displaying nocturnal acrophases. These findings suggest that daily rhythms in extraretinal non-visual opsins may be transducing daily photic cycles and contributing to the entrainment of locomotor activity and other light-synchronised rhythms in blind cavefish species.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111772"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological effects of research handling on the northern elephant seal (Mirounga angustirostris)","authors":"Lauren A. Cooley ,&nbsp;Allyson G. Hindle ,&nbsp;Cassondra L. Williams ,&nbsp;Paul J. Ponganis ,&nbsp;Shawn M. Hannah ,&nbsp;Holger Klinck ,&nbsp;Markus Horning ,&nbsp;Daniel P. Costa ,&nbsp;Rachel R. Holser ,&nbsp;Daniel E. Crocker ,&nbsp;Birgitte I. McDonald","doi":"10.1016/j.cbpa.2024.111771","DOIUrl":"10.1016/j.cbpa.2024.111771","url":null,"abstract":"<div><div>Wildlife researchers must balance the need to safely capture and handle their study animals to sample tissues, collect morphological measurements, and attach dataloggers while ensuring their results are not confounded by stress artifacts caused by handling. To determine the physiological effects of research activities including chemical immobilization, transport, instrumentation with biologgers, and overnight holding on a model marine mammal species, we collected hormone, blood chemistry, hematology, and heart rate data from 19 juvenile northern elephant seals (<em>Mirounga angustirostris</em>) throughout a translocation experiment. Across our six sampling timepoints, cortisol and aldosterone data revealed a moderate hormonal stress response to handling accompanied by minor changes in hematocrit and blood glucose, but not ketone bodies or erythrocyte sedimentation rate. We also examined heart rate as a stress indicator and found that interval heart rate, standard deviation of heart rate, and apnea-eupnea cycles were influenced by handling. However, when seals were recaptured after several days at sea, all hormonal and hematological parameters had returned to baseline levels. Furthermore, 100 % of study animals were resighted in the wild post-translocation, with some individuals observed over four years later. Together, these findings suggest that while northern elephant seals exhibit measurable physiological stress in response to handling, they recover rapidly and show no observable long-term deleterious effects, making them a robust species for ecological and physiological research.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111771"},"PeriodicalIF":2.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of larval rearing temperature on steroidogenesis pathway development in winter flounder (Pseudopleuronectes americanus) early life history","authors":"Nina Bellenger ,&nbsp;Étienne Audet-Walsh ,&nbsp;Lucas Germain ,&nbsp;Eric Normandeau ,&nbsp;Céline Audet","doi":"10.1016/j.cbpa.2024.111770","DOIUrl":"10.1016/j.cbpa.2024.111770","url":null,"abstract":"<div><div>Winter flounder (<em>Pseudopleuronectes americanus</em>) is a North Atlantic flatfish that inhabits cold-water environments already affected by global warming. Flatfishes are particularly sensitive during their juvenile stage to a phenomenon known as temperature-dependent sex determination (TSD). In this study, we hypothesized that many genes involved in the steroidogenesis pathway are already expressed at the larval stage in winter flounder and that temperature conditions may influence this pathway prior to the juvenile stage, which is usually considered the TSD-sensitive period. We also hypothesized that temperature effects on the steroidogenesis pathway may carry over from the larval to the juvenile stage. We surveyed three cohorts issued from three different spawning events during spring. Rearing temperature and salinity conditions followed seasonal conditions in the St. Lawrence Estuary (Québec, Canada). As a result, cohorts were exposed to different temperature regimes during egg and larval development. Once metamorphosis occurred, all juveniles were held at a stable temperature of 10.1 ± 0.8 °C. Larvae were sampled at 20, 30, and, 40 days post-hatching and juveniles at one and two months post-metamorphosis. RNA was extracted from these individuals and then sequenced. Transcriptome analysis showed clear differentiation between larvae and juveniles. Gene expression patterns showed that the steroidogenesis pathway, including genes involved in gonadal differentiation, was influenced by temperature during larval development, which indicates that sexual determination at the cellular level is an early process in winter flounder development. Carry-over effects of larval rearing temperature conditions were present at the juvenile stage, with an increased number of genes involved in the steroidogenesis pathway being affected. Altogether, our study highlights the important role of larval development and temperature exposure on the transcriptome of winter flounder.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111770"},"PeriodicalIF":2.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of triiodothyronine on the immune response and extracellular matrix remodeling during zebrafish heart regeneration","authors":"Reece R.B. Long ,&nbsp;Oliver M.N. Bullingham ,&nbsp;Benjamin Baylis ,&nbsp;Jared B. Shaftoe ,&nbsp;John R. Dutcher ,&nbsp;Todd E. Gillis","doi":"10.1016/j.cbpa.2024.111769","DOIUrl":"10.1016/j.cbpa.2024.111769","url":null,"abstract":"<div><div>Damage to the human heart is an irreparable process that results in a permanent impairment in cardiac function. There are, however, a number of vertebrate species including zebrafish (<em>Danio rerio</em>) that can regenerate their hearts following significant injury. In contrast to these regenerative species, mammals are known to have high levels of thyroid hormones, which has been proposed to play a role in this difference in regenerative capacity. However, the mechanisms through which thyroid hormones effect heart regeneration are not fully understood. Here, zebrafish were exposed to exogenous triiodothyronine (T₃) for two weeks and then their hearts were damaged through cryoinjury to investigate the effect of thyroid hormones on ECM remodeling and the components of the immune response during heart regeneration. Additionally, cardiac fibroblasts derived from trout, another species of fish known to display cardiac regenerative capacity, were exposed to T₃ <em>in vitro</em> to analyze any direct effects of T₃ on collagen deposition. It was found that cryoinjury induction results in an increase in myocardial stiffness, but this response was muted in T₃ exposed zebrafish. The measurement of relevant marker gene transcripts suggests that T₃ exposure reduces the recruitment of macrophages to the damaged zebrafish heart immediately following injury but had no effect on the regulation of collagen deposition by cultured trout fibroblasts. These results suggest that T₃ effects both the immune response and ECM remodeling in zebrafish following cardiac injury.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111769"},"PeriodicalIF":2.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological responses of euryhaline marine fish to naturally-occurring hypersalinity","authors":"Andrew J. Esbaugh","doi":"10.1016/j.cbpa.2024.111768","DOIUrl":"10.1016/j.cbpa.2024.111768","url":null,"abstract":"<div><div>Hypersaline habitats are generally defined as those with salinities in excess of 40 ppt. Well-known hypersaline regions (e.g. salt and soda lakes) have a well-earned reputation for being among the most inhospitable habitats in the world, and fish endemic to these areas have been the subject of much research related to extremophile physiology. Yet, marine coastal hypersalinity is both a common occurrence and a growing consideration in many marine coastal ecosystems, in part owing to human influence (e.g. evaporation, river diversion, desalination effluent). Importantly, any increase in salinity will elevate the osmoregulatory challenges experienced by a fish, which must be overcome by increasing the capacity to imbibe and absorb water and excrete ions. While great attention has been given to dynamic osmoregulatory processes with respect to freshwater to seawater transitions, and to the extreme hypersalinity tolerance that is associated with the adoption of an osmo-conforming strategy, relatively little focus has been placed on the physiological implications of moderate hypersalinity exposures (e.g. ≤ 60 ppt). Importantly, these exposures often represent the threshold of osmoregulatory performance owing to energetic constraints on ion excretion and efficiency limitations on water absorption. This review will explore the current state of knowledge with respect to hypersalinity exposure in euryhaline fishes, while placing a particular focus on the physiological constraints, plasticity and downstream implications of long-term exposure to moderate hypersalinity.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111768"},"PeriodicalIF":2.1,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive effort affects cellular response in the mantle of Nodipecten subnodosus scallops exposed to acute hyperthermia","authors":"A. Marzari ,&nbsp;I.S. Racotta ,&nbsp;C. Escobedo-Fregoso ,&nbsp;S. Artigaud ,&nbsp;E. Kraffe ,&nbsp;R.L. Salgado-García","doi":"10.1016/j.cbpa.2024.111766","DOIUrl":"10.1016/j.cbpa.2024.111766","url":null,"abstract":"<div><div>In marine ecosystems, temperature regulates the energy metabolism of animals. In the last decades, the temperature increase was related to mass mortality events of marine ectotherms, particularly during high-energy investment for reproduction. In scallops, the mantle has been poorly investigated while this tissue covers more than 40 % of the body mass, contributing to the perception of surrounding environmental stimuli. Our aim was to assess the cellular and molecular responses linked to energy metabolism in the mantle of adult <em>N. subnodosus</em> facing acute hyperthermia during reproductive effort. Scallops collected in spring (late gametogenesis) and summer (ripe gonads) were exposed to a control temperature (22 °C) or acute hyperthermia (30 °C) for 24 h. In spring, increased arginine kinase (AK) activity together with increased pyruvate kinase/citrate synthase ratio (PK/CS) suggested an enhanced carbohydrate, pyruvate, and arginine metabolism to maintain the adenylate energy charge (AEC) in the mantle of scallops coping with acute thermal increase. In summer, animals decreased their AEC (5 %) and arginine phosphate pool (40 %) and increased their anaerobic metabolism as shown by enhanced activities of lactate-dehydrogenase (LDH) and octopine dehydrogenase (ODH), respectively. The abundance of twenty proteins involved in energy metabolism (isocitrate dehydrogenase, ATP synthase subunit β), protein protection (cognate heat shock protein 70), and cytoskeleton (actins and tubulins) were affected only by season. These results underlie the role of the mantle of <em>N. subnodosus</em> in the seasonal responses of this tissue to thermal fluctuations during reproductive effort with possible implications for the physiological performance of scallops under heat waves in wild or harvest conditions.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"299 ","pages":"Article 111766"},"PeriodicalIF":2.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142481386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}