Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Study on the mechanism of skeletal deformity of Lateolabrax maculatus induced by low temperature stress","authors":"Lin Pan ,&nbsp;Yonghang Zhang ,&nbsp;Yun Li,&nbsp;Haishen Wen,&nbsp;Xiaoqiang Liu,&nbsp;Jing Yang,&nbsp;Xin Qi,&nbsp;Kaiqiang Zhang","doi":"10.1016/j.cbd.2025.101592","DOIUrl":"10.1016/j.cbd.2025.101592","url":null,"abstract":"<div><div>Low-temperature culturing leads to skeletal deformities in juvenile spotted sea bass (<em>Lateolabrax maculatus</em>), reducing survival rate and indeed significantly impacting the aquaculture industry. Aimed to investigating the molecular mechanisms behind cold-induced skeletal deformities, in the present study, histological analysis was performed on the bones of spotted sea bass cultured at low and normal temperatures, with deformity rates assessed using the Alizarin Red staining method. It was found that the spotted sea bass cultured at low temperatures exhibited varying degrees of skeletal deformities in different regions, particularly in the spinal. Normal and deformity spinal were compared by transcriptome analysis to show the impact of the low temperature. To further investigate how the deformity formatted, convex and concave side of the spinal were separated and compared by transcriptome analysis as well. The enrichment results indicated that cold exposure may induce oxidative stress and activate the PPAR pathway, and further activated the inflammation-related NF-κB signaling pathway. The upregulation of v-Rel within the NF-κB pathway modulates the TGF-β signaling pathway, contributing to asymmetrical spinal development. Furthermore, we reveal that cold-induced inflammatory pathways may drive long-term physiological changes in bone structure through extracellular matrix (ECM) remodeling, ultimately leading to persistent skeletal abnormalities. Additionally, cold stress results in decreased expression of the <em>foxo3</em> gene, which reduces stress-induced cell death and promotes bone stability. This study establishes a comprehensive molecular framework elucidating how cold stress modulates both metabolic homeostasis and osseous integrity, thereby driving environmentally mediated developmental asymmetries.</div></div><div><h3>Summary sentence</h3><div>Low-temperature stress potentially induces skeletal deformities in spotted sea bass through oxidative stress-mediated activation of the PPAR/NF-κB signaling pathway and downregulation of <em>foxo3</em> expression.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101592"},"PeriodicalIF":2.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, characterization, and expression analysis reveal regulatory roles of MCM genes in Patinopecten yessoensis under low-pH stress","authors":"Fanhua Sun ,&nbsp;Sihua Kou ,&nbsp;Huan Liao ,&nbsp;Zhengrui Zhang ,&nbsp;Jingjing Kong ,&nbsp;Xindong Cui ,&nbsp;Zujing Yang ,&nbsp;Zhenmin Bao ,&nbsp;Xiaoting Huang","doi":"10.1016/j.cbd.2025.101591","DOIUrl":"10.1016/j.cbd.2025.101591","url":null,"abstract":"<div><div>The Yesso scallop (<em>Patinopecten yessoensis</em>), an ecomically important bivalve species, exhibits high susceptibility to ocean acidification. The growth retardation induced by low-pH stress poses a significant challenge for Yesso scallop aquaculture. However, the molecular mechanisms underlying this phenomenon are not well understood. Considering the pivotal role of cell proliferation in organism growth, we investigated the minichromosome maintenance (MCM) family in <em>P. yessoensis</em>, which are key regulators of DNA replication initiation and cell cycle regulation. In this study, we identified nine MCM genes (<em>PyMCM2–10</em>) in the <em>P. yessoensis</em> genome. These <em>PyMCMs</em> exhibit highly conserved sequence characteristics and typical MCM domains. Phylogenetic analysis showed that <em>PyMCMs</em> cluster into nine distinct clades, underscoring their strong evolutionary conservation across species homologs. Spatiotemporal expression profiling demonstrated widespread expressions of <em>PyMCMs</em> throughout all developmental stages and adult tissues, with particularly high levels in vigorous cell proliferation (<em>e.g.</em>, up to 318 TPM in multicell stage and up to 202 TPM in gonad tissue). Notably, <em>PyMCM6</em> exhibited consistently high expression across development and across tissues (&gt; 44 TPM), suggesting a key regulatory role in both development and tissue maintenance. Under low-pH stress, the expressions of <em>PyMCMs</em> were downregulated to varying degrees, with <em>PyMCM5</em> showing the most significant reduction (|log₂FC| up to 3.5), while <em>PyMCM8</em> and <em>PyMCM9</em> remained relatively stable. This pattern suggests a strategic response that scallops reduce DNA replication capacity (mediated by <em>PyMCM2–7</em>) but potentially maintain DNA repair functions (associated with <em>PyMCM8/9</em> stability) to mitigate damage induced by low-pH, potentially explaining the intrinsic inhibition of cell proliferation. Quantitative real-time PCR and <em>in situ</em> hybridization further confirmed that low-pH stress inhibits <em>PyMCMs</em> expressions (<em>p</em> &lt; 0.05), with the effect amplified as pH decreases. Collectively, these findings enhance our understanding of <em>PyMCMs</em> in regulating bivalve growth retardation under low-pH stress and provide valuable insights into the mechanisms of environmental adaptation in bivalves.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101591"},"PeriodicalIF":2.2,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of low salinity stress on the taurine metabolism and transport in the Iwagaki oyster Crassostrea nippona","authors":"Jianwen Gong ,&nbsp;Qi Li","doi":"10.1016/j.cbd.2025.101593","DOIUrl":"10.1016/j.cbd.2025.101593","url":null,"abstract":"<div><div>Intensified extreme weather events driven by climate change have exacerbated salinity fluctuations in intertidal zones, threatening the survival of marine organisms. As a stenohaline species, the response mechanisms of Iwagaki oyster (<em>Crassostrea nippona</em>) to low-salinity stress are crucial for understanding the adaptability of intertidal biota. Integrative proteomic and metabolomic analyses were conducted to elucidate the regulatory mechanisms of taurine metabolism and transport under low-salinity stress. High-performance liquid chromatography (HPLC) was employed to validate taurine content changes in gill tissues, while in vivo and in vitro experiments combined with a yeast heterologous expression system were used to characterize the functional properties of the taurine transporter (TauT). The study revealed significant enrichment of the taurine metabolic pathway under low-salinity conditions. Altered expressions of key enzymes including cysteinesulfinic acid decarboxylase (CSAD) and cysteine dioxygenase (CDO) and TauT indicated that taurine-mediated osmotic regulation and antioxidant defense relied on extracellular NaCl concentration-dependent transport rather than osmotic pressure. The findings provide a theoretical foundation for developing low-salinity-tolerant oyster strains and managing intertidal ecosystems.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101593"},"PeriodicalIF":2.2,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144748953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic identification and functional characterization of the ACTIN gene family in relation to gonadal development and gametogenesis in the Pacific oyster (Crassostrea gigas)","authors":"Chunxue Zheng ,&nbsp;Hong Yu ,&nbsp;Qi Li","doi":"10.1016/j.cbd.2025.101588","DOIUrl":"10.1016/j.cbd.2025.101588","url":null,"abstract":"<div><div>The <em>Actin</em> gene family plays critical roles in cellular processes such as cytoskeletal organization and gametogenesis. In this study, a comprehensive genomic analysis of the <em>Actin</em> gene family in the Pacific oyster (<em>Crassostrea gigas</em>) was conducted, with a focus on its involvement in gonadal development. A total of 37 <em>Actin</em> genes were identified (including 19 <em>Actin</em>, 11 ARPs and 7 ALPs), which were classified into 14 subfamilies and were unevenly distributed across eight chromosomes. Gene structure analysis revealed significant variation in exon count, ranging from 2 to 41, and highly conserved motifs. Transcriptomic analysis across various tissues demonstrated that most <em>Actin</em> genes were broadly expressed, while certain genes exhibited gonad-specific expression. Notably, <em>CgAct3c</em>, <em>CgAct4</em>, and <em>CgAct5-1</em> were significantly expressed during the development of male gonads, while <em>CgArp4</em> and <em>CgActl3-2</em> were significantly enriched during the development of female gonads, indicating their involvement in the occurrence of germ cells. Additionally, expression patterns varied across larval developmental stages, indicating diverse roles of <em>Actin</em> genes in early development. These findings provide valuable insights the molecular mechanisms governing gonadal maturation and gametogenesis in <em>C. gigas</em> and contribute to a deeper understanding of Actin proteins in bivalves.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101588"},"PeriodicalIF":2.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsporidian infection disrupts gut microbiome and drives metabolic dysregulation in Macrobrachium nipponense","authors":"Zhengfeng Ding ,&nbsp;Yang Wu ,&nbsp;Shijie Yang ,&nbsp;Xiangyue Xia","doi":"10.1016/j.cbd.2025.101589","DOIUrl":"10.1016/j.cbd.2025.101589","url":null,"abstract":"<div><div>The gut microbiome plays a crucial role in maintaining host health, yet its functions in aquatic animals remain incompletely understood. Given its economic significance in Asian aquaculture, the oriental river prawn (<em>Macrobrachium nipponense</em>) was chosen to assess microbiome dynamics during infection with <em>Potaspora macrobrachium</em>, an emerging microsporidian pathogen. Histopathological analysis confirmed severe microsporidian infections in diseased prawns. Using 16S rRNA gene sequencing (Illumina MiSeq), we characterized infection-induced shifts in the gut microbiome's composition and structure. Bacterial communities showed significant divergence between health states, with infection status being the primary clustering determinant. The microbiome was dominated by <em>Proteobacteria</em> (86.4 %), <em>Bacteroidota</em> (6.89 %), <em>Firmicutes</em> (3.41 %), and <em>Actinobacteriota</em> (1.69 %), displaying distinct abundance patterns. Disease-state indicators included <em>Rikenellaceae</em>, <em>Pseudomonas oryzihabitans</em>, and <em>Bacteroides fragilis</em> among others. Critically, functional prediction revealed that metabolic pathways, particularly carbohydrate metabolism, amino acid metabolism, and cofactor/vitamin metabolism, were significantly altered in infected prawns, suggesting that <em>P. macrobrachium</em> infection reprograms the metabolic activity of the gut microbiome.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101589"},"PeriodicalIF":2.2,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated transcriptome, proteome and targeted metabolome analysis reveals genes and steroids related to gonadal development in Ruditapes philippinarum","authors":"Kaiyue Huang,&nbsp;Yimeng Chen,&nbsp;Yijin Gao,&nbsp;Haichun Liu,&nbsp;Zhiying Gao,&nbsp;Yang Liu,&nbsp;Zhongming Huo,&nbsp;Yanjie Qin","doi":"10.1016/j.cbd.2025.101587","DOIUrl":"10.1016/j.cbd.2025.101587","url":null,"abstract":"<div><div>Gonadal development in <em>Ruditapes philippinarum</em> involves germ cell formation, sex differentiation, vitellogenesis, and maturation, regulated by complex genes-hormone interactions. To elucidate these mechanisms, multi-omics analyses (transcriptomics, proteomics, and steroid targeted metabolomics) and qPCR were applied to gonads across developmental stages in clams. Differential expression analysis revealed 332 testicular and 560 ovarian genes, alongside 174 testicular and 107 ovarian proteins. Integrated omics identified 12 concordantly annotated gene-protein pairs. Expressed levels for 14 genes (8 transcriptome only, 6 multi-omics) demonstrated that <em>Cyclin B2</em> (<em>CCNB2</em>), <em>Moloney sarcoma oncogene</em> (<em>MOS</em>), and <em>Glutaminase</em> (<em>GLS</em>) peaked during the maturation stage and significantly declined during the partially spent stage (<em>P</em> &lt; 0.05). These three genes were also more highly expressed in the ovary compared to the testis (<em>P</em> &lt; 0.05). <em>Cell Division Cycle 20</em> (<em>CDC20</em>), Atrial Natriuretic Peptide Receptor Type A (<em>ANPRA</em>), and Rpsr-1 showed highest levels during both maturation and partially spent stage (<em>P</em> &lt; 0.05), with <em>ANPRA</em> predominantly expressed in the testis, and <em>CDC20</em> and Rpsr-1 in the ovary; <em>Calmodulin</em> (<em>CALM</em>), <em>Glutamine Synthetase</em> (<em>GLUL</em>), Sushi Domain Containing 2 (SUSD2), Rpsr-2, Rpsr-3, Rpsr-4 and CD151 Molecule (Raph Blood Group) (CD151) attained their highest values in the partially spent stage (<em>P</em> &lt; 0.05), where Rpsr-3, Rpsr-4 and CD151 expressed higher in the testis (<em>P</em> &lt; 0.05), and <em>CALM</em>, <em>GLUL</em>, SUSD2 and Rpsr-2 were more pronounced in the ovary (<em>P</em> &lt; 0.05); <em>Phospholipase A2 Group IVA</em> (<em>PLA2G4</em>) exhibited its highest expression during the growing stage, gradually declining as development progressed, which was significantly higher in the testis than in the ovary (<em>P</em> &lt; 0.05). Targeted metabolomics identified 14 steroids in clam gonads, with five steroids—Pregnenolone, 5α-Pregnane-3,20-dione, 17α-Hydroxypregnenolone, 25-Hydroxycholesterol, and 24-dehydro Cholesterol—elevated during the gonadal growing phase (no sex differences). Intriguingly, 17β-estradiol (typically reported in bivalve gonad development) was undetected. Integrated transcriptomic/proteomic analyses confirmed absence of its synthesis pathway, including key enzymes like cytochrome P450 aromatase (CYP19A1). This study established a multi-omics platform to detect the genes-hormone interactions, and evaluate expression dynamics of 14 key regulators across gonadal stages and sexes in <em>R philippinarum</em>, concurrently evaluating steroid hormones roles. These findings provide valuable resources for further research into the comprehensive regulatory mechanisms governing gonad development in clams.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101587"},"PeriodicalIF":2.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative proteomic analysis of Fujian oyster (Crassostrea angulata) with different cadmium accumulation capabilities","authors":"Shixing Fu ,&nbsp;Longping Wu ,&nbsp;Yiming Feng ,&nbsp;Yizhou Ke ,&nbsp;Huayong Que ,&nbsp;Bo Shi","doi":"10.1016/j.cbd.2025.101581","DOIUrl":"10.1016/j.cbd.2025.101581","url":null,"abstract":"<div><div>Cadmium (Cd) can be transmitted by the food chain and biomagnification, leading serious health risks to humans. It is a major metal pollutant in marine environments. Oysters, being the most widely farmed shellfish globally, have an exceptional ability to accumulate and tolerate Cd. Research has shown that there are significant individual differences in the ability of oysters to accumulate Cd, although their potential molecular basis and key regulatory mechanisms are still unclear. This study utilized 4D-label-free quantitative proteomics to compare individuals within the same Fujian oyster population exhibiting significant difference in Cd accumulation (2–5 times higher or lower Cd levels). It identified 946 differentially expressed proteins (DEPs) totally. Calcium-binding proteins such as calmodulin (CaM) and phospholipid-transporting ATPase ABCA1 were significantly upregulated in high-accumulating oysters, likely providing more cellular binding sites for Cd and enhancing its sequestration, thereby increasing Cd accumulation in oyster tissues. Glutathione S-transferases (GSTs) and heat shock 70 kDa protein 12A (Hspa12a) play key roles in Cd detoxification and cellular antioxidant defense, particularly in oysters with low Cd accumulation. Thus, the varying expression of proteins related to Cd sequestration and detoxification is a crucial factor underlying the variability in Cd accumulation capacity among Fujian oysters. This study illuminates how oysters exhibit differential Cd accumulation at a molecular level. Monitoring marine environmental pollution and guaranteeing the safety of seafood will likewise be crucial.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101581"},"PeriodicalIF":2.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadal sex differentiation in Eleutheronema tetradactylum: Histological features and transcriptomic insights from mature gonads","authors":"Evodia Moses Mkulo ,&nbsp;Lukman Iddrisu ,&nbsp;Salifu Ibrahim ,&nbsp;Jiansheng Huang ,&nbsp;Linjuan Wang ,&nbsp;Anna Zheng ,&nbsp;Happiness Moses Mwemi ,&nbsp;Jin Minxuan ,&nbsp;Huijuan Zhang ,&nbsp;Baogui Tang ,&nbsp;Qian Ma ,&nbsp;Kwaku Amoah ,&nbsp;Bei Wang ,&nbsp;Zhongliang Wang","doi":"10.1016/j.cbd.2025.101586","DOIUrl":"10.1016/j.cbd.2025.101586","url":null,"abstract":"<div><div>Plasticity in teleosts is a fascinating evolutionary strategy enables species to acclimate to environmental changes. However, the molecular mechanisms that underlie gonadal differentiation and reversal remain incompletely understood. The four-finger threadfin (<em>Eleutheronema tetradactylum</em>), a protandrous hermaphrodite belonging to the Polynemidae family, serves as an excellent model for studying genes and molecules involved in gonadal transformation. In this study, we used de novo transcriptome sequencing of the male and female gonads to identify key regulatory genes and pathways involved in sex differentiation. A total of 12,034 DEGs were identified, with 7567 upregulated and 4467 downregulated between testes and ovaries. GO and KEGG enrichment analyses identified steroid hormone biosynthesis as the key pathway in gonadal differentiation. Several novel sex biased genes DEGs were identified, including testis-biased genes <em>nefl</em>, <em>rfesd</em>, and <em>gpatch8</em>, and ovary-biased genes <em>ift57</em>, <em>hoxb5a</em>, <em>naxd</em>, and <em>tgfbr3</em>. furthermore, clusters of testis-specific genes, including <em>dnah7</em>, <em>tspan1</em>, <em>hspa8</em>, <em>dnah3</em>, <em>dnah6</em>, <em>dnah17</em>, <em>myo10</em>, <em>kcnq3</em>, <em>dnaaf11</em>, <em>rtase</em>, <em>dmrt1</em>, <em>smcl1b</em>, <em>slc44a5b</em>, <em>tex11</em>,<em>cyp51</em> and ovary-biased genes, such as <em>cd9</em>, <em>zp2</em>, <em>ccnp</em>, <em>cldnd</em>, <em>znf622</em>, <em>tc1a</em>, <em>hsd17b7</em>, foxl2, <em>sox3</em>, and <em>sox19a</em> were identified, emphasizing their crucial roles in gonadal differentiation. This in-depth transcriptomic study sheds light on the molecular foundations of reproductive adaptability, offering novel insights for disciplines such as aquaculture, conservation and evolutionary biology.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101586"},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling reveals sex-specific gene expression patterns in Eriocheir sinensis gonads","authors":"Feng Zhao ,&nbsp;Haoyu Liu ,&nbsp;Yaxuan Shi ,&nbsp;Longlong Fu ,&nbsp;Huixia Feng ,&nbsp;Cong Zhang ,&nbsp;Kai Zhang ,&nbsp;Shaowu Yin ,&nbsp;Xianhui Ning","doi":"10.1016/j.cbd.2025.101584","DOIUrl":"10.1016/j.cbd.2025.101584","url":null,"abstract":"<div><div>The Chinese mitten crab (<em>Eriocheir sinensis</em>) exhibits prominent sexual dimorphism with important economic implications, yet the molecular mechanisms underlying its sex determination and regulation remain poorly characterized. Here, we present the first comparative gonadal transcriptome analysis of <em>E. sinensis</em> to identify sex-related genes. High-throughput sequencing of testicular and ovarian tissues identified 5894 differentially expressed genes (DEGs), including 2581 female-related and 3313 male-related DEGs. Functional enrichment analyses revealed critical pathways governing crustacean sexual regulation, such as Steroid hormone biosynthesis pathway and GnRH signaling pathway. Moreover, a protein-protein interaction (PPI) network of 65 sexual regulation-related DEGs was constructed. Accordingly, Estradiol 17-beta-dehydrogenase 8 (<em>HSD17B8</em>), a dual-function enzyme in estrogen/testosterone metabolism, and Heat shock protein 90 (<em>HSP90</em>), a molecular chaperone stabilizing estrogen receptors, were identified as hub genes. In addition, experimental validation via RT-qPCR showed strong concordance with transcriptomic data, confirming the reliability of sex-biased expression patterns. The results of this study provide valuable resource of target genes for future studies on the molecular mechanisms underlying sex determination and regulation in <em>E. sinensis</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101584"},"PeriodicalIF":2.2,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and characterization of heat shock protein superfamily in the cotton aphid Aphis gossypii: phylogenetic, structural, and expression profiles analyses","authors":"Tianxing Jing ,&nbsp;Chenyang Li ,&nbsp;Yanjin Zhang ,&nbsp;Peixuan Li ,&nbsp;Jin Yang ,&nbsp;Honghua Su ,&nbsp;Shuai Zhang","doi":"10.1016/j.cbd.2025.101583","DOIUrl":"10.1016/j.cbd.2025.101583","url":null,"abstract":"<div><div>The cotton aphid, <em>Aphis gossypii</em>, belonging to Hemiptera Aphididae is a worldwide pest on various plants. This pest is particularly notorious for its high tolerance to both temperature and insecticides. Heat shock proteins (HSPs), which have been recognized as molecular chaperones, play pivotal roles in the response of insects to environmental stress. However, information on <em>HSP</em> genes in <em>A. gossypii</em> and their expression response remain limited. In this study, 50 <em>HSP</em> genes were identified in the <em>A. gossypii</em> genome, including 3 <em>HSP90s</em>, 15 <em>HSP70s</em>, 11 <em>HSP60s</em>, 19 <em>HSP40s</em> and 2 <em>sHSPs</em>. Phylogenetic analysis strongly supported the family classification of <em>A. gossypii HSP</em> genes. All of the <em>AgHSPs</em> were distributed across four chromosomes, and several gene duplication events were observed. Transcriptome data revealed that most <em>AgHSPs</em> could be induced by short-term high temperature treatment. Conversely, most <em>HSPs</em> decreased their transcription levels under long-term high temperature exposure. Notably, three <em>HSPs</em> (<em>AgHSP40-9-X2, AgHSP40-15</em> and <em>AgHSP70-8</em>) were consistently up-regulated after insecticide exposure in both susceptible and resistant populations. Moreover, the expressions of <em>HSPs</em> across several morphs of aphids were systematically evaluated for the first time. Our findings provide fundamental data for future studies and enhance our understanding of the roles of <em>HSPs</em> in environmental stress adaptation in <em>A. gossypii</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101583"},"PeriodicalIF":2.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}