Gonadal sex differentiation in Eleutheronema tetradactylum: Histological features and transcriptomic insights from mature gonads

IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Evodia Moses Mkulo , Lukman Iddrisu , Salifu Ibrahim , Jiansheng Huang , Linjuan Wang , Anna Zheng , Happiness Moses Mwemi , Jin Minxuan , Huijuan Zhang , Baogui Tang , Qian Ma , Kwaku Amoah , Bei Wang , Zhongliang Wang
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引用次数: 0

Abstract

Plasticity in teleosts is a fascinating evolutionary strategy enables species to acclimate to environmental changes. However, the molecular mechanisms that underlie gonadal differentiation and reversal remain incompletely understood. The four-finger threadfin (Eleutheronema tetradactylum), a protandrous hermaphrodite belonging to the Polynemidae family, serves as an excellent model for studying genes and molecules involved in gonadal transformation. In this study, we used de novo transcriptome sequencing of the male and female gonads to identify key regulatory genes and pathways involved in sex differentiation. A total of 12,034 DEGs were identified, with 7567 upregulated and 4467 downregulated between testes and ovaries. GO and KEGG enrichment analyses identified steroid hormone biosynthesis as the key pathway in gonadal differentiation. Several novel sex biased genes DEGs were identified, including testis-biased genes nefl, rfesd, and gpatch8, and ovary-biased genes ift57, hoxb5a, naxd, and tgfbr3. furthermore, clusters of testis-specific genes, including dnah7, tspan1, hspa8, dnah3, dnah6, dnah17, myo10, kcnq3, dnaaf11, rtase, dmrt1, smcl1b, slc44a5b, tex11,cyp51 and ovary-biased genes, such as cd9, zp2, ccnp, cldnd, znf622, tc1a, hsd17b7, foxl2, sox3, and sox19a were identified, emphasizing their crucial roles in gonadal differentiation. This in-depth transcriptomic study sheds light on the molecular foundations of reproductive adaptability, offering novel insights for disciplines such as aquaculture, conservation and evolutionary biology.

Abstract Image

四趾棘龙性腺的性别分化:成熟性腺的组织学特征和转录组学见解
硬骨鱼的可塑性是一种迷人的进化策略,使物种能够适应环境变化。然而,性腺分化和逆转的分子机制仍然不完全清楚。四指丝线虫(Eleutheronema tetradactylum)是雌雄同体,属于蓼科,是研究性腺转化的基因和分子的一个很好的模型。在这项研究中,我们对雄性和雌性性腺进行了从头转录组测序,以确定参与性别分化的关键调控基因和途径。共鉴定出12034个deg,其中睾丸和卵巢之间上调7567个,下调4467个。GO和KEGG富集分析发现类固醇激素的生物合成是性腺分化的关键途径。发现了几个新的性别偏倚基因DEGs,包括睾丸偏倚基因nefl、rfesd和gpatch8,以及卵巢偏倚基因ift57、hoxb5a、naxd和tgfbr3。此外,我们还发现了dnah7、tspan1、hspa8、dnah3、dnah6、dnah17、myo10、kcnq3、dnaaf11、rtase、dmrt1、smcl1b、slc44a5b、tex11、cyp51等睾丸特异性基因簇和cd9、zp2、ccnp、cldnd、znf622、tc1a、hsd17b7、foxl2、sox3和sox19a等卵巢偏倚基因簇,强调了它们在性腺分化中的重要作用。这项深入的转录组学研究揭示了生殖适应性的分子基础,为水产养殖、保护和进化生物学等学科提供了新的见解。
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来源期刊
CiteScore
5.10
自引率
3.30%
发文量
69
审稿时长
33 days
期刊介绍: Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.
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