Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Understanding the spleen response of Russian sturgeon (Acipenser gueldenstaedtii) dealing with chronic heat stress and Aeromonas hydrophila challenge","authors":"Alicia Costábile ,&nbsp;Gonzalo Paredes ,&nbsp;Marcio Aversa-Marnai ,&nbsp;Carmen Lorenzo ,&nbsp;Diana Pérez Etcheverry ,&nbsp;Mauricio Castellano ,&nbsp;Ignacio Quartiani ,&nbsp;Daniel Conijeski ,&nbsp;Alejandro Perretta ,&nbsp;Andrea Villarino ,&nbsp;Ana María Ferreira ,&nbsp;Valeria Silva-Álvarez","doi":"10.1016/j.cbd.2024.101352","DOIUrl":"10.1016/j.cbd.2024.101352","url":null,"abstract":"<div><div>Sturgeon aquaculture has grown in recent years, driven by increasing global demand for its highly valued products. Russian sturgeon (<em>Acipenser gueldenstaedtii</em>), recognised as one of the most valuable species for caviar production, is farmed in several warm-temperate regions. However, the substantial temperature increase due to global warming represents a challenge for developing sturgeon aquaculture. Previously we demonstrated that Russian sturgeon under chronic heat stress (CHS) exhibited a liver metabolic reprogramming to meet energy demands, weakening their innate defences and leading to increased mortality and economic losses. Here, we used RNA-seq technology to analyse regulated genes in the spleen of Russian sturgeons exposed to CHS and challenged with <em>Aeromonas hydrophila.</em> The assembly gave 253,415 unigenes, with 13.7 % having at least one reliable functional annotation. We found that CHS caused mild splenitis and upregulated genes related to protein folding, heat shock response, apoptosis and autophagy while downregulated genes associated with the cell cycle. The cell cycle arrest was maintained upon <em>A. hydrophila</em> challenge in heat-stressed fish, potentially inducing cell senescence. Surprisingly, immunoglobulin heavy and light chains were upregulated in the spleen of stressed sturgeons but not in those maintained at tolerable temperatures; however, no changes in IgM serum levels were observed in any condition. Our findings indicate that long-term exposure to non-tolerable temperatures induced a heat shock response and activated apoptosis and autophagy processes in the spleen. These mechanisms may enable the control of tissue damage and facilitate the recycling of cell components in a condition where the nutrient supply by the liver might be insufficient. Stressed sturgeons challenged with <em>A. hydrophila</em> maintain these mechanisms, which could culminate in cellular senescence.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101352"},"PeriodicalIF":2.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of the moso bamboo pest, Hippotiscus dorsalis, reveals differentially expressed unigenes across various tissues","authors":"Mingzhen Zhao ,&nbsp;MengJiao Chen ,&nbsp;Hong Qiu ,&nbsp;Jieyu Wu ,&nbsp;Lulu Dai ,&nbsp;Shaoyong Zhang","doi":"10.1016/j.cbd.2024.101354","DOIUrl":"10.1016/j.cbd.2024.101354","url":null,"abstract":"<div><div><em>Hippotiscus dorsalis</em> (Hemiptera: Pentatomidae) is a common pest that feeds on the stems of Moso bamboo. Therefore, investigating the molecular processes related to its fundamental physiology is important. In this study, transcriptome sequencing was employed to compare gene expression in different tissues of male and female adults. A total of 36,238 annotated unigenes were identified through the NR database and compared with those of other known species. Among these, <em>Halyomorpha halys</em> and <em>Nezara viridula</em> showed the highest homology with <em>H. dorsalis</em>, with a total similarity exceeding 86 %. The top significant differentially expressed genes were mainly associated with protein digestion, carbohydrates metabolism, as well as reproduce regulation between sexes. The Gene Ontology enrichment analysis across three different segments of each sex revealed some terms associated with detoxification such like IMP biosynthetic process and acetyl-CoA metabolic process. Additionally, Kyoto Encyclopedia of Genes and Genomes enrichment analysis highlighted significant pathways related to glucose metabolism and pancreatic secretion. Further investigation of these DEGs, particularly in the calcium signaling pathway and glycan biosynthesis and metabolism, is essential for understanding sugar metabolism and transport in phytophagous insects.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101354"},"PeriodicalIF":2.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remodeling of the epigenetic landscape in rainbow trout, Oncorhynchus mykiss, offspring in response to maternal choline intake","authors":"Khalid Freij ,&nbsp;Beth Cleveland ,&nbsp;Peggy Biga","doi":"10.1016/j.cbd.2024.101348","DOIUrl":"10.1016/j.cbd.2024.101348","url":null,"abstract":"<div><div>This project focused on evaluating the effects of maternal dietary choline intake on global DNA methylation profiles and related transcriptional changes in rainbow trout offspring. Three experimental diets were formulated to test different levels of choline intake: (a) 2065 ppm choline (Low Choline, 0 % supplementation), (b) 5657 ppm choline (Medium Choline, 0.6 % supplementation), and (c) 9248 ppm choline (High Choline, 1.2 % choline supplementation). Six rainbow trout families were fed experimental diets beginning 18 months post-hatch until spawning; their offspring were fed a commercial diet. Reduced representation bisulfite sequencing (RRBS) was utilized to measure genome-wide methylation in offspring immediately after hatching. When comparing to the Medium Choline offspring, differential DNA methylation occurred more in the Low Choline offspring than High Choline, especially in genic features like promoters. The differentially methylated CpGs (q ≤ 0.01) were identified evenly between CpG islands and shores in the genome, mostly found in the introns of genes. Genes such as <em>fabp2</em> and <em>leap2B</em> associated with protein binding, fatty acid binding, DNA binding, and response to bacteria were differentially methylated and detected as differentially regulated genes by previous RNA-seq analysis. Although these findings indicate that levels of dietary choline available in broodstock diets alter offspring DNA methylation;, most differentially expressed genes were not associated with differential DNA methylation, suggesting additional mechanisms playing a role in regulating gene expression in response to maternal choline intake.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101348"},"PeriodicalIF":2.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and integrative analysis of miRNA-mRNA response to salinity stress in Oreochromis mossambicus cells","authors":"Zhi Luo ,&nbsp;Yuxing Huang ,&nbsp;Jinquan Fan,&nbsp;Erchao Li,&nbsp;Liqiao Chen,&nbsp;Xiaodan Wang","doi":"10.1016/j.cbd.2024.101350","DOIUrl":"10.1016/j.cbd.2024.101350","url":null,"abstract":"<div><div>This study investigated the genetic response of tilapia (<em>Oreochromis mossambicus</em>) brain cells to hypertonic stress, focusing on miRNAs regulation. Three hundred and thirty-one known miRNAs and 163 novel miRNAs which responded to hypertonic stress were identified by high-throughput sequencing in tilapia brain cells. Differential expression analysis revealed that 16 miRNAs were significantly upregulated, while 11 miRNAs were significantly downregulated. These differentially expressed miRNAs are closely related to metabolism, immune response, and neural regulation. The target genes of these miRNAs are implicated in neurotrophic and synaptic signaling pathways, potentially affecting metabolic and apoptotic processes. GO and KEGG enrichment analyses provided insights into the biological processes and pathways affected by hypertonic stress. Furthermore, correlation analysis between mRNA and miRNA highlighted miRNA-mRNA interactions related to cell cycle and apoptosis regulation. These results indicated significant changes of miRNA expression under hypertonic stress and their crucial role in osmotic pressure regulation. This study offers a basis for further exploration of miRNA functions and molecular mechanisms in tilapia, potentially informing practices for aquaculture in challenging environments such as saline-alkaline waters.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101350"},"PeriodicalIF":2.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the adaptation mechanism of different color morphs of sea cucumber Apostichopus japonicus to light intensities from the perspective of metabolomics","authors":"Bohong Liu ,&nbsp;Shilin Liu ,&nbsp;Lina Sun ,&nbsp;Lili Xing","doi":"10.1016/j.cbd.2024.101346","DOIUrl":"10.1016/j.cbd.2024.101346","url":null,"abstract":"<div><div>Global warming has multi-dimensional and complex impacts on the Earth's system, among which changes in light intensities cannot be overlooked. Sea cucumbers are a marine biological resource with significant economic and ecological value. Their presence and activity help maintain the balance and stability of marine ecosystems. The variation in light intensities have important ecological effects on sea cucumbers. Light intensities can alter the synthesis and degradation of metabolic substances within the bodies of <em>Apostichopus japonicus</em> by changing their body color. Their changes affect the production of microorganisms in the environment, thereby achieving the goal of bioremediation. This study investigated metabolic variations in green, purple, and white sea cucumber <em>Apostichopus japonicus</em> under different light conditions (0 lx and 910 lx) with a 12-h light and 12-h dark photoperiod. The findings indicated that the sea cucumbers displayed more diverse metabolic alterations under 910 lx illumination compared to 0 lx. Specifically, these color morphs primarily responded to changes in light intensities through “tryptophan metabolism” and “biosynthesis of steroid hormones”. Additionally, high light intensities environment exacerbated the consumption of fatty acids by sea cucumbers. Different color morphs of sea cucumbers have differences in key metabolites in response to changes in light intensities. Green and white sea cucumbers primarily adapt to environment through phospholipids, while purple sea cucumbers mainly utilize fatty acids. These results enhance our comprehension of how sea cucumbers adapt ecologically to varying light intensities, and they offer valuable insights for systematically uncovering the regulatory processes that marine animals employ in response to environmental changes.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101346"},"PeriodicalIF":2.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of gonadotropin on certain testis-related genes identified through testicular transcriptome analysis in the Asian and the African catfish","authors":"Sonika Kar ,&nbsp;Swathi Tenugu ,&nbsp;Akanksha Pranoty ,&nbsp;Narlagiri Anusha ,&nbsp;Uday Kumar Udit ,&nbsp;Prabhaker Sangem ,&nbsp;Balasubramanian Senthilkumaran","doi":"10.1016/j.cbd.2024.101349","DOIUrl":"10.1016/j.cbd.2024.101349","url":null,"abstract":"<div><div>Induction of spawning in catfish is well demonstrated in females while stimulation of spermiation in males seems difficult. This has led to least understanding of gene profile changes during testicular maturation. Expression of the factors after hormonal induction that control reproductive processes has become an intense research area in fish endocrinology. In view of this, <em>de novo</em> assemblies of testicular transcriptomes of the Asian catfish, <em>Clarias batrachus</em> and the African catfish, <em>C. gariepinus,</em> were performed to identify genes/factors involved in testicular maturation. For testicular induction, human chorionic gonadotropin (hCG) was administered <em>in vivo</em> to both the catfish species during active phase of reproduction. 1,68,071 and 1,26,232 assembled transcripts were obtained from <em>C. batrachus</em> and <em>C. gariepinus</em> testicular transcriptomes and were annotated using different databases. Further, <em>in silico</em> analysis suggested the presence of several transcripts that were involved in steroidogenesis and male reproduction. Comparison of transcriptomes of both species revealed the presence of certain unique genes related to reproduction differentially. Expression profiles after hCG induction in testis showed higher expression of certain steroidogenesis related genes such as <em>star</em>, <em>cyp11b1</em>, <em>cyp17a</em> and <em>cyp21a</em> in both the species. Further, expression levels of crucial factors related to testis, such as <em>dmrt1/2/3,</em> were also found to be significantly higher after gonadotropin induction. However, <em>amh</em>, <em>tgfα</em> and <em>dmrt4/5</em> expression levels were significantly low. Factors related to male reproduction <em>i.e.</em>, <em>tekt1</em>, <em>tekt2</em>, <em>sox9</em>, <em>spag1</em>, <em>spata2</em> and <em>spata7</em> were found to be differentially upregulated in hCG treated testicular tissues of both the species. Histology analysis indicated that the gonadotropin induction either short or long term is a better strategy to highlight expression profile changes during testicular maturation in teleost.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101349"},"PeriodicalIF":2.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis uncovers the expression of genes associated with growth in the gills and muscles of white shrimp (Litopenaeus vannamei) with different growth rates","authors":"Wenhao Wang ,&nbsp;Junrou Huang ,&nbsp;Wenyu Fang ,&nbsp;Hongyun Zhang ,&nbsp;Zhiqiang Chen ,&nbsp;Jianguo Lu","doi":"10.1016/j.cbd.2024.101347","DOIUrl":"10.1016/j.cbd.2024.101347","url":null,"abstract":"<div><div><em>Litopenaeus vannamei</em> is a crucial species in aquaculture. The gene expression patterns associated with distinct growth rates are not well understood. To investigate this, we used RNA-seq to study the underlying growth mechanism of <em>L. vannamei</em> with varying growth rates. Individuals of higher growth performance (HG), middle growth performance (MG), and lower growth performance (LG) were examined. A total of 8422 and 4560 differentially expressed genes (DEGs) were identified in gill and muscle samples, respectively. Genes related to growth were significantly up-regulated in HG gills, such as cuticle protein, chitin synthase, pupal cuticle protein, titin myosin G heavy chain, and myosin heavy chain 10. The GO enrichment analysis revealed that the DEGs of HG gills were significantly enriched in “structural constituent of cuticle”, “primary metabolic process” and “chitin binding”. The growth-related genes were highly expressed in HG muscle, such as myosin heavy chain, myosin heavy chain type A and myosin 3. The GO enrichment analysis revealed that the DEGs of HG muscle were significantly enriched in “myosin filament”, “myosin complex” and “myofibril”. These findings provide insights into mechanisms underlying the growth performance of superior <em>L. vannamei,</em> and identify candidate genes for genetic improvement programs aimed at enhancing this trait.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101347"},"PeriodicalIF":2.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of high-protein feeds on growth, free amino acid metabolism and protein metabolism-related genes in larvae and juveniles of rice flower carp (Procypris merus)","authors":"Ruijie Guo ,&nbsp;Kai Huang ,&nbsp;Kai Yu ,&nbsp;Fei Xue ,&nbsp;Yixin Liang ,&nbsp;Xuhong Yang ,&nbsp;Jiao Huang ,&nbsp;Yaoting Wu ,&nbsp;Dandan Wang","doi":"10.1016/j.cbd.2024.101345","DOIUrl":"10.1016/j.cbd.2024.101345","url":null,"abstract":"<div><div>The effect of dietary protein on fish is widely studied. However, the high-protein diet effects and mechanisms on growth and amino acid metabolism in <em>Procypris merus</em> remain unclear. In this study, we investigated the effect of dietary protein levels (38 %, 44 %, 50 %) on the growth performance and amino acid contents in larvae and juveniles of <em>P. merus</em>. Transcriptome sequencing was used to study the adaptation mechanism of <em>P. merus</em> to a high-protein diet. The final length, specific growth rate, and protein efficiency ratio were remarkably decreased with increasing dietary protein levels, while the amino acid content of the body was significantly increased. In addition, 370,513,858 reads were obtained and assembled into 278,939 unigenes, with an average length of 559 bp. KEGG analysis revealed that differentially expressed genes were mainly involved in protein digestion and absorption, carbohydrate digestion and absorption, starch and sucrose metabolism, and pancreatic secretion. Moreover, high-protein increased the expression of genes involved in trypsin (<em>prss</em>, <em>ctrl</em>, <em>cpa</em>, <em>cpb</em>), peptide transporter (<em>pept</em>), amino acid transporter (<em>b0at1</em>), and gluconeogenesis (<em>g6pase</em>), which could digest and absorb the dietary protein, and converse amino acids into energy, resulting in adaption to a high-protein diet.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101345"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of intestinal structure, enzyme activity, intestinal microbiota and gene expression in different segments of pufferfish (Takifugu Obscurus)","authors":"Shengli Fu ,&nbsp;Kun Qian ,&nbsp;Xiao Tu ,&nbsp;Jie Lu ,&nbsp;Tuo Yao ,&nbsp;Lingtong Ye ,&nbsp;Jianmin Ye","doi":"10.1016/j.cbd.2024.101341","DOIUrl":"10.1016/j.cbd.2024.101341","url":null,"abstract":"<div><div>The structure of fish intestines does not have a clear regional division, while the function of the intestines may be related to their structure. Therefore, in this study, the delimitation of intestinal segments in pufferfish (<em>Takifugu obscurus</em>) was achieved by morphological analysis. Subsequently, enzyme activity, intestinal microbiota, and gene expression were examined to compare the differences among the pufferfish various segments. According to four morphological parameters: height of mucosa folds (HF), width of mucosa folds (WF), thickness of muscularis (TM), and cross-sectional area (CSA), the pufferfish's intestine was divided into anterior intestine (AI), middle intestine (MI), and posterior intestine (PI). The activity levels of amylase, lipase, and trypsin in the AI and MI were significantly higher than these in the PI. According to the analysis of 16S rDNA, the dominant microbiota at the phylum level in the different segments were Epsilonbacteraeota, Spirochaetes, and Proteobacteria. At the genus level, there were variations observed in the relative abundance of Brevinema, Mycobacterium, Bradyrhizobium, and Microvirga. <em>α</em> diversity analysis revealed that the richness indexes (Ace and Chao1) were the lowest in the MI, while <em>β</em> diversity analysis revealed significant difference in intestinal microbial community composition among the three intestinal segments. Furthermore, RNA-Seq was used to identify differential expression genes (DEGs) and biological pathways among the different intestinal segments. The DEGs between the AI and MI were enriched in pancreatic secretion and protein digestion and absorption, those between AI and PI were involved in ascorbate and aldarate metabolism and glutathione metabolism, and those between MI and PI were involved in steroid biosynthesis, fat digestion and absorption, vitamin digestion and absorption, and glycine, serine and threonine metabolism. In conclusion, the presented results compare and analyze the differences in various intestinal segments of pufferfish, which will be conductive to future exploration of the functions of these different segments.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101341"},"PeriodicalIF":2.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142483143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of sexually dimorphic cypris larvae of the rhizocephalan barnacle Peltogasterella gracilis","authors":"Asami Kajimoto ,&nbsp;Kenji Toyota ,&nbsp;Tsuyoshi Ohira ,&nbsp;Yoichi Yusa","doi":"10.1016/j.cbd.2024.101342","DOIUrl":"10.1016/j.cbd.2024.101342","url":null,"abstract":"<div><div><em>Peltogasterella gracilis</em> (Cirripedia: Rhizocephala), a crustacean parasite of hermit crabs, displays genotypic sex determination. Its larvae are planktonic, and female larvae settle on the host. Subsequently, the females control the host's behavior by spreading a root-like structure called “the interna” within the host's body, and form sacs containing eggs called “the externae” outside the host's body. On the other hand, male larvae settle on immature externae and become dwarf males. The cypris larvae of <em>P. gracilis</em> show sexual dimorphism in size and morphology. However, there is no understanding of the molecular mechanisms underlying the sexual dimorphism observed at the larval stage. Here, we conducted a transcriptome analysis and compared the expression of genes in male and female cyprids to better understand their sexual differentiation and settlement processes. A total of 2870 differentially expressed transcripts, comprising 456 female- and 2414 male-biased transcripts were identified. Among the male-biased ones, ionotropic glutamate receptor-, heat shock protein-, acetylcholine-, and homeobox-, cuticle-related transcripts were included. Additionally, 29 gene ontology terms were associated with the sex-specific traits. The present study improves our understanding of sex determination, sexual differentiation, and settlement processes of rhizocephalans.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"52 ","pages":"Article 101342"},"PeriodicalIF":2.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}