Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"1H NMR-based metabolomic analysis of hypersalinity-induced oviparity in brine shrimp","authors":"Wei-Yi Lee ,&nbsp;Chen-Hsun Liu ,&nbsp;Bo-Hua Yu ,&nbsp;Yung-Kuo Lee ,&nbsp;Chiu-Hui Kuo ,&nbsp;Cheng- Yi Huang ,&nbsp;Chang Yu-Teng ,&nbsp;Zi-Yan Xu ,&nbsp;Kuohsun Chiu","doi":"10.1016/j.cbd.2024.101409","DOIUrl":"10.1016/j.cbd.2024.101409","url":null,"abstract":"<div><div>This study investigated the mechanisms by which high salinity conditions stimulate adult <em>Artemia</em> females to produce diapaused cysts. We used a ¹H NMR-based metabolomic approach to elucidate the metabolic regulation between ovoviviparity and oviparity in <em>Artemia</em> exposed to different salinities. At a salinity of 80 ppt, 100 % of females produced diapaused cysts, compared to 20 % at 50 ppt. Metabolic profiling revealed significant alterations in a range of metabolites, including 5,6-dihydrouracil, betaine, and malate, in females undergoing oviparity at 80 ppt compared to ovoviviparity at 30 ppt. Multivariate statistical analyses indicated clear separation between the two reproductive strategies. The up-regulated metabolites in oviparity were involved in significant metabolic pathways, such as β-alanine metabolism and the citrate cycle, highlighting substantial metabolic differences between the two reproductive strategies. These identified metabolic pathways might play crucial roles in the maternal response to high salinity, facilitating embryo protection and enhancing the survival and reproductive success of brine shrimp. These findings provide a basis for further research into the molecular mechanisms underlying <em>Artemia</em> adaptation to high salinity environments.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101409"},"PeriodicalIF":2.2,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome wide analysis of the sox32 gene in germline maintenance and differentiation in leopard coral grouper (Plectropomus leopardus)","authors":"Mingyi Wang ,&nbsp;Hui Ding ,&nbsp;Mingjian Liu ,&nbsp;Yurui Gao ,&nbsp;Lin Li ,&nbsp;Chaofan Jin ,&nbsp;Zhenmin Bao ,&nbsp;Bo Wang ,&nbsp;Jingjie Hu","doi":"10.1016/j.cbd.2024.101402","DOIUrl":"10.1016/j.cbd.2024.101402","url":null,"abstract":"<div><div>The Sox family genes, as a group of transcription factors, are widely expressed in vertebrates and play a critical role in reproduction and development. The present study reported that 26 <em>sox</em> genes were identified from the genome and transcriptome of <em>P. leopardus.</em> The phylogenetic tree construction, chromosome localization, and gene structure analysis were executed to verify the evolutionary relationships, gene duplication, and deletion variations of <em>P. leopardus sox</em> genes in evolution. The sequence alignment revealed the HMG-box domain was highly conserved throughout the Sox gene family. The expression profile showed expression levels of <em>sox</em> genes showed tissue specificity. The dimorphic expression pattern of most <em>sox</em> genes in intersex and adult gonads was also observed, suggesting an important role of <em>sox</em> genes for sex differentiation in <em>P. leopardus</em>. Notably, <em>sox32</em> was specifically highly expressed in gonadal tissues and might play a novel role within the gonads. The fluorescent in situ hybridization (FISH) showed <em>sox32</em> mRNA was detected in germ stem cells and oocytes of different stages, and lowly expressed in sertoli cells. In testis, <em>sox32</em> was not detected in male germ cells. Our results provided new insights into the <em>sox32</em> that might be involved in gonadal development and differentiation in <em>P. leopardus</em>. To sum up, this study comprehensively analyzed the Sox gene family of <em>P. leopardus</em> and provided new insights into the function of <em>sox</em> genes, which could potentially revolutionize our understanding of the mechanisms of sex determination, sex differentiation, and reproductive development in fish.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101402"},"PeriodicalIF":2.2,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome-derived evidence reveals the regulatory network in the skeletal muscle of the fast-growth mstnb−/− male tilapia","authors":"You Wu ,&nbsp;Yiyun Du ,&nbsp;Yanbin Zhang ,&nbsp;Maolin Ye,&nbsp;Deshou Wang,&nbsp;Linyan Zhou","doi":"10.1016/j.cbd.2024.101405","DOIUrl":"10.1016/j.cbd.2024.101405","url":null,"abstract":"<div><div>Myostatin (Mstn) negatively regulates muscle growth and Mstn deficiency induced “double-skeletal muscle” development in vertebrates, including tilapias. In this study, we performed a transcriptomic analysis of skeletal muscle from both wild-type and <em>mstnb</em>^−/− males to investigate the molecular mechanisms underlying skeletal muscle hypertrophy in <em>mstnb</em>^−/− mutants. We identified 4697 differentially expressed genes (DEGs), 113 differentially expressed long non-coding RNAs (DE lncRNAs), 211 differentially expressed circular RNAs (DE circRNAs), and 98 differentially expressed microRNAs (DE miRNAs). The DEGs were significantly enriched in proteasome and ubiquitin-mediated proteolysis pathways. Cis- and trans-targeting genes of DE lncRNAs were also notably enriched in the above two pathways. The putative host genes of DE circRNAs linked to myofibrils, contractile fibers, and so on. Additionally, DE miRNAs were associated with ubiquitin-mediated proteolysis and key signaling pathways, including AMPK, FoxO, and mTOR. Furthermore, the core competing endogenous RNA (ceRNA) network was constructed comprising 31 DEGs, 37 DE miRNAs, 14 DE circRNAs, and 45 DE lncRNAs. The key roles of ubiquitin-proteasome system were highlighted in the ceRNA network. Taken together, this study provides a novel perspective on muscle mass increase in Mstn mutants through the repression of protein degradation and facilitates our understanding of the molecular mechanisms of skeletal muscle hypertrophy in fish.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101405"},"PeriodicalIF":2.2,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular responses of paddy field carp (Cyprinus carpio) in the agricultural heritage to major environmental factors in paddy fields","authors":"Fangcheng Li ,&nbsp;Xiangbing Cheng ,&nbsp;Xumeng He,&nbsp;Gilbert Kumilamba,&nbsp;Jiayi Liao,&nbsp;Jiangwei Cao,&nbsp;Qigen Liu,&nbsp;Jiamin Sun","doi":"10.1016/j.cbd.2024.101410","DOIUrl":"10.1016/j.cbd.2024.101410","url":null,"abstract":"<div><div>As a core element of the Globally Important Agricultural Heritage System (GIAHS), the Qingtian paddy field carp (<em>Cyprinus carpio</em>, PF-carp) has been domesticated for over 1200 years in paddy field environments. This species has successfully adapted to shallow-water conditions in paddy fields. To reveal the adaptation mechanism, we conducted transcriptome sequencing on the hepatopancreas of PF-carp under two temperature conditions (28 °C and 38 °C) and concurrently analysed RNA-seq data from hypoxic conditions in the same tissue. By analysing high-temperature transcriptome data, 3154 differentially expressed genes (DEGs) were identified. KEGG analysis indicated that DEGs involved various pathways, including protein processing in endoplasmic reticulum, circadian rhythm, and HIF-1 signaling pathway. Notably, protein processing in endoplasmic reticulum was significantly enriched with key genes such as HSP70, HSP90, HSP40, CNX, CRT, and Bip. Through concurrent analysis of RNA-seq data from hypoxic conditions, we found that PF-carp regulate their metabolism through multiple pathways and produce almost opposite metabolic regulation to adapt to high temperature and hypoxic environments. The opposite activation state observed in the HIF-1 signaling pathway is particularly intriguing. In conclusion, PF-carp appear to rely on protein processing in endoplasmic reticulum to maintain cell homeostasis at high temperatures. The HIF-1 signaling pathway may emerged as a key player in adapting PF-carps to paddy fields. This study provides valuable insights into the adaptive mechanisms of domesticated fish in paddy fields.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101410"},"PeriodicalIF":2.2,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the gut microbiota and metabolome of Lateolabrax japonicus: A multi-omics approach","authors":"Gang Zhou ,&nbsp;Ying-si Wang ,&nbsp;Hui-juan Wang,&nbsp;Ting-li Sun,&nbsp;Ru-qun Peng,&nbsp;Su-juan Li,&nbsp;Hong Peng,&nbsp;Xia Wen,&nbsp;Xiao-xuan Zhu,&nbsp;Yun-qi Zhu,&nbsp;Zong-bin Cui,&nbsp;Qing-shan Shi,&nbsp;Xiao-bao Xie","doi":"10.1016/j.cbd.2024.101408","DOIUrl":"10.1016/j.cbd.2024.101408","url":null,"abstract":"<div><div>The intestinal microbiota plays a crucial role in the health and development of fish, engaging in intricate interactions with the host organism. As a significant species in aquaculture, <em>Lateolabrax japonicus</em> serves as an exemplary model for investigating these interactions and their subsequent effects on growth and health. This study utilized a multi-omics approach, incorporating metagenomic sequencing and non-targeted metabolomics, to delineate the gut microbiota and metabolome of <em>L. japonicus</em> throughout various developmental phases. Collected from a meticulously controlled aquaculture setting, the intestinal microbiota of L. <em>japonicus</em> underwent high-throughput sequencing to scrutinize microbial DNA and enumerate metabolites. The metagenomic analysis uncovered a heterogeneous gut microbiota in L. <em>japonicus</em>, predominantly composed of Proteobacteria and Firmicutes, with marked heterogeneity in microbial composition across developmental stages. A particularly noteworthy discovery was the prevalence of the genus <em>Acinetobacter</em>, which may significantly influence health and disease resistance. The metabolomic profiling discerned 4479 metabolites, each exhibiting pronounced stage-specific metabolic signatures, particularly within lipid, amino acid, and energy metabolism pathways. The correlation analysis between microbiota and metabolites highlighted the substantial impact of specific genera, such as <em>Acinetobacter</em> and <em>Gaeumannomyces</em>, on the metabolic milieu. This study provides a comprehensive overview of the dynamic shifts in the gut microbiota and metabolome of L. <em>japonicus</em>, highlighting stage-specific transitions that could be pivotal for refining aquaculture practices. The findings underscore the complex interdependence between microbiota composition and metabolic function, providing valuable insights into the modulation of fish health and growth.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101408"},"PeriodicalIF":2.2,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative proteomic analysis reveals the response mechanism of freshwater leech (Whitmania pigra) under heat-stress challenge","authors":"Shuaibing Wang ,&nbsp;Xiaochuan Zheng ,&nbsp;Quan Wang ,&nbsp;Aimin Wang ,&nbsp;Haihua Wang ,&nbsp;Huiyun Fu ,&nbsp;Mange Kaunda ,&nbsp;Liangwei Xiong ,&nbsp;Bo Liu","doi":"10.1016/j.cbd.2024.101406","DOIUrl":"10.1016/j.cbd.2024.101406","url":null,"abstract":"<div><div>Temperature is an environmental parameter that remarkably affects the survival and organism health of poikilothermal animal-<em>Whitmania pigra Whitman</em>. Heat stress destroys the physiological homeostasis of intestine tissue. However, no studies on the intestinal mucosa response of leech exposure to heat stress have been reported so far. To identify the biomarker proteins involved in heat stress response, we performed a Tandem mass tag (TMT)-based comparative proteomic analysis on leech's intestine after exposing to 27 °C and 35 °C. The cumulative mortality in the 35 °C heat stress group increased on the third day after stress compared with the control group. Mean-while, intestinal malondialdehyde (MDA) content was significantly up-regulated whereas total antioxidant capacity (T-AOC) was significantly down-regulated. A total of 3935 proteins were identified through proteomic analysis. Heat stress resulted in 144 differential proteins; 75 were up-regulated, and 69 were down-regulated. The differential expression proteins in response to heat stress are mainly involved in calcium-binding proteins, molecular chaperones, cytoskeleton integrity, immune function and inflammation response, DNA damage and repair, ribosomal protein synthesis, stress hormones and neuro-transmitters, tumorigenesis, and apoptosis. Protein-protein network interaction analysis showed that HSP, CD8A, C3, CD63, CD81, MRPL58, MRPL15, HNRNPA1, AMBP, IGHEP1, SERPINC1, CYP3A4, ATP12A, RPS13, CaM, HSP70 binding protein, HSPA6, C4A, ANXA7, ARG1, ARG2, ATP1B, CALCA, HTR1B, and GNGT1 are the critical proteins of leeches resistance to heat stress. Our results systematically provide substantial responsive candidate proteins combating heat stress and enhance our understanding of the intrinsic response mechanisms of thermal sensitivities animal <em>W. pigra</em> facing heat exposure.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101406"},"PeriodicalIF":2.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis identified genes involved in ovarian development in Takifugu rubripes","authors":"Mingtao Hu ,&nbsp;Hongwei Yan ,&nbsp;Jinfeng Chen ,&nbsp;Rui Gao ,&nbsp;Weiyuan Li ,&nbsp;Huiting Zhou ,&nbsp;Jia Wang ,&nbsp;Qi Liu ,&nbsp;Xiuli Wang ,&nbsp;Pengfei Hu ,&nbsp;Chuang Fu","doi":"10.1016/j.cbd.2024.101407","DOIUrl":"10.1016/j.cbd.2024.101407","url":null,"abstract":"<div><div>Ovarian development is a complex process involving multiple genes, but the molecular mechanisms underlying this process in <em>Takifugu rubripes</em> remain poorly understood. This study aimed to identify genes associated with ovarian development in <em>T. rubripes</em> and to investigate the regulatory mechanisms of oocyte maturation. Transcriptome data were compared across four different developmental stages (stage II to V) to identify differentially expressed genes (DEGs) and perform GO and KEGG enrichment analysis. The expression patterns of randomly selected genes were then validated by qPCR. The results yielded a total of 1,289,401,820 raw data from all libraries, with 16,929 DEGs identified across all comparison groups. The DEGs were predominantly enriched in ovarian steroidogenesis, estrogen-mediated signaling, and TGF-beta signaling pathways. The qPCR analysis showed that <em>cyp17a1</em> was identified as being expressed at similar levels in stage II and III. Thereafter, <em>cyp17a1</em> was observed to undergo a continuous increase in expression from stage III to V. <em>cyp19a1</em>, <em>nanos1</em>, <em>foxl2</em> and <em>ar</em> were identified as being expressed at similar levels at stage II and III, then increase in expression from stage III to IV and subsequent downregulation from stage IV to V. <em>hsd17b1</em> was identified as being expressed at similar levels at stage II and IV. This study represents a transcriptomic study of ovarian development in female <em>T. rubripes</em>. Several essential ovarian-related genes and sex-related biological pathways were identified. The results will improve our understanding of the molecular mechanisms underlying ovarian development in this species.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101407"},"PeriodicalIF":2.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ocean warming and acidification alter calcification and innate immune system gene expression in juvenile American lobsters, Homarus americanus","authors":"Christine San Antonio ,&nbsp;Helen Poynton ,&nbsp;Keegan Krick ,&nbsp;Robyn Hannigan","doi":"10.1016/j.cbd.2024.101404","DOIUrl":"10.1016/j.cbd.2024.101404","url":null,"abstract":"<div><div>The Gulf of Maine, home to American lobster, <em>Homarus americanus,</em> is experiencing rapid ocean warming (OW) and acidification (OA) due to climate change. While some studies have investigated the effects of either ocean acidification (OA) or warming (OW) on lobsters, few explore the interaction of these stressors, particularly on gene expression. We evaluated the effects of OA and OW on early benthic juvenile lobster transcriptomics using RNA sequencing and RT-qPCR through two distinct aquarium experiments. Lobsters were reared under OW/OA conditions aligned with values predicted for 2100: decrease in pH by 0.3–0.4 units; mean sea surface warming of 2.89 °C. RNA was isolated from carapace hypodermal tissue in both experiments. The multi-stressor treatment in the RNAseq experiment had the greatest differential expression. Genes of interest pertaining to calcification and cuticle development were primarily downregulated under high temperature but upregulated under acidified and multi-stressor conditions. In the RT-qPCR experiment, crustin alone was significantly downregulated and only under the most extreme multi-stressor treatment. This gene along with the prophenoloxidase activating enzyme had expression that trended toward downregulation across all treatments, suggesting a possible correlation to immune suppression. Expression profiles for crustin and the calcification gene, carbonic anhydrase differed across treatments based on molt cycle timing, indicating that stressor impacts may vary depending on the molt cycle phase. Elevated temperature had a greater effect on the expression of calcification and cuticle development genes, though the direction of expression reversed with multiple stressors. These results indicate the impacts of OW and OA on early benthic juvenile lobsters are complex, possibly synergistic, vary with molt cycle, and potentially interfere with normal cuticle development, which may increase susceptibility to injury or disease.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101404"},"PeriodicalIF":2.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis reveals the cerebral sex-specific responses to chronic hypoxia in yellow catfish (Pelteobagrus fulvidraco)","authors":"Danqing Yin ,&nbsp;Cheng Zhao","doi":"10.1016/j.cbd.2024.101401","DOIUrl":"10.1016/j.cbd.2024.101401","url":null,"abstract":"<div><div>Hypoxia disrupts multiple physiological processes, including metabolism, immunity, and reproduction in teleosts. The brain plays a critical role in adapting to environmental changes, regulating the endocrine system, and controlling reproduction. The present study investigated the sex-specific cerebral responses to chronic hypoxia through an integrated analysis of the transcriptome, proteome, and metabolome of yellow catfish. Common cerebral responses in both females and males included activation of the HIF signaling pathway, angiogenesis, and improved oxygen delivery by red blood cells. Reproductive defects were indicated by the downregulation of <em>gh1</em>, <em>cga</em>, and <em>tshb</em> in both sexes. Thyroid hormone homeostasis was more severely disrupted by hypoxia in females than in males, accompanied by a significant decrease in the level of VTG in the female brain. Damaged brain function was evidenced by the highly enriched pathways of “cytokine-cytokine receptor interaction” and “ECM-receptor interaction,” and the blood-brain barrier (BBB) also appeared to be disrupted in female fish. In the male brain, reproductive-related genes or proteins, including <em>prl</em>, <em>lepr</em>, and AVP, were specifically decreased. Dysfunction in the male brain was also indicated by the enrichment of pathways such as “cytokine-cytokine receptor interaction” and “neuroactive ligand-receptor interaction,” based on differentially expressed genes (DEGs) and proteins (DEPs). Additionally, chronic hypoxia appeared to inhibit cerebral amino acid metabolism in males. In summary, our results offer insight into understanding the sex-specific cerebral responses induced by chronic hypoxia in teleosts.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101401"},"PeriodicalIF":2.2,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of the response of Spodoptera frugiperda (Lepidoptera: Noctuidae) to short-term low-temperature stress","authors":"Huawei Ren,&nbsp;Junrui Zhi,&nbsp;Dingyin Li,&nbsp;Wenbo Yue,&nbsp;Li Liu","doi":"10.1016/j.cbd.2024.101394","DOIUrl":"10.1016/j.cbd.2024.101394","url":null,"abstract":"<div><div><em>Spodoptera frugiperda</em> is a major invasive pest that poses a serious threat to crops worldwide. Low temperature is a key factor limiting the survival and reproduction for this pest. To study the responses of <em>S. frugiperda</em> to low-temperature stress, high-throughput sequencing was used to perform transcriptomic analysis on the 6^th instar larvae under low-temperature stress at 5 °C and 10 °C, along with 25 °C as a control. As a result, 215 differentially expressed genes (DEGs) were identified under different low-temperature stresses. Upon functional annotation of the DEGs in KEGG and GO databases, the number of DEGs annotated in control vs. LT10 comparison was the largest (<em>n</em> = 150), whereas fewer DEGs (<em>n</em> = 89) were annotated in control vs. LT5 comparison. This discrepancy suggested that <em>S. frugiperda</em> might adopt different strategies to cope with low-temperature stress. The DEGs in the GO database were particularly associated with cell catalytic activity, cell anatomical entity process, cell apoptosis, and cell binding channel. KEGG annotation analysis of the different low-temperature stresses showed that most of the enriched pathways were related to carbon metabolism, oxidative phosphorylation, and lipid metabolism. The results will be the basis for mastering the cold tolerant mechanism of <em>S. frugiperda</em>, and is of great significance for its prevention.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101394"},"PeriodicalIF":2.2,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}