Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Functional study of RNA alternative splicing in zebrafish adaptation to low temperature","authors":"Jiazhen Li ,&nbsp;Wenhao Li ,&nbsp;Huihua Lu ,&nbsp;Yongpeng Zhao ,&nbsp;Zhengchao Zhu ,&nbsp;Liangbiao Chen","doi":"10.1016/j.cbd.2025.101656","DOIUrl":"10.1016/j.cbd.2025.101656","url":null,"abstract":"<div><div>Alternative splicing (AS) is crucial for gene expression regulation during cold adaptation, yet its role in fish remains unclear. This study revealed tissue-specific AS patterns in zebrafish, with the gill exhibiting the highest AS frequency, followed by the skin, ovary and brain. Cold exposure significantly altered splicing sites across tissues, with exon skipping (SE) being the predominant AS form. Alternative splicing genes (ASGs) displayed strong tissue specificity, and splicing variability correlated with temperature, suggesting AS acts as a temperature-sensitive regulatory mechanism. Splicing factors, such as <em>srsf7a</em>, notably undergo AS, demonstrating their responsiveness to temperature changes. Key genes exhibiting differential splicing, including <em>setd7</em> and <em>mapta</em>, present temperature-dependent isoforms. Under conditions of cold stress, zebrafish <em>setd7</em> experiences cold-induced AS, resulting in the exclusion of exon 2 and the production of a truncated protein with a disrupted SET domain. This splicing event enhances zebrafish activity at lower temperatures. Simultaneously, <em>mapta</em> produces a 5R tau isoform via exon 7 skipping, which may dynamically regulate the balance between microtubule stability and plasticity during cold adaptation. Our transcriptome-wide analysis elucidates the AS regulatory mechanisms underlying the zebrafish response to cold stress and identifies key AS-mediated genes involved in cold adaptation, thereby providing a theoretical foundation for the molecular breeding of cold-tolerant aquaculture species.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101656"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145411123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism study of selenium nanoparticles alleviating heat stress in rainbow trout hepatocytes by regulating lncRNA expression","authors":"Jun Sun ,&nbsp;Zhe Liu","doi":"10.1016/j.cbd.2025.101691","DOIUrl":"10.1016/j.cbd.2025.101691","url":null,"abstract":"<div><div>This study investigated the role of selenium nanoparticles (SeNPs) in modulating long non-coding RNA (lncRNA) expression and their regulatory networks in rainbow trout hepatocytes under heat stress using transcriptomic and bioinformatic approaches. Exposure to 5 μg/mL SeNPs alleviated heat stress-induced damage, with trans-target prediction identifying 1360 differentially expressed lncRNAs (DElncRNAs) and 4355 differentially expressed mRNAs (DEmRNAs). A competing endogenous RNA (ceRNA) network was constructed based on DElncRNAs, DEmiRNAs, and DEmRNAs with Pearson correlation coefficients &gt;0.99 and <em>P</em> &lt; 0.05, ultimately comprising 37 DElncRNAs, 18 DEmiRNAs, and 14 DEmRNAs. Functional enrichment analysis revealed that target genes of DElncRNAs were significantly enriched in biological processes related to stimulus response, immune regulation, and antioxidant activity. KEGG pathway analysis indicated that these genes are primarily involved in endoplasmic reticulum signaling, potentially activating downstream MAPK, IL-17, B-cell receptor, and TNF signaling pathways. These regulatory mechanisms may enhance thermal tolerance in rainbow trout hepatocytes by suppressing apoptosis and promoting cell proliferation. Our findings highlight the critical role of SeNP-mediated lncRNA regulatory networks in mitigating heat stress, providing novel insights into the molecular basis of stress resilience in fish.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101691"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145579404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain proteomics of rainbow trout at certain maturation stages: biological insights and fish by-products processing perspectives","authors":"A.A. Tsekova,&nbsp;A.E. Kuritsyn,&nbsp;S.A. Murzina","doi":"10.1016/j.cbd.2025.101669","DOIUrl":"10.1016/j.cbd.2025.101669","url":null,"abstract":"<div><div>For the first time, the brain proteomic profiles of rainbow trout <em>Oncorhynchus mykiss</em> (Walbaum, 1792) at two maturation stages were described and discussed. Maturation and reproduction are critical stages in the fish life cycle, but the biochemical and morphophysiological processes underlying these stages are not well understood. The reactions that occur in the fish brain during maturation, regulating the reproductive process and accompanying metabolic changes, are of particular interest. <em>O. mykiss</em> is an economically significant commercial species of salmonid fish that is a model organism for biological research. We analyzed the brain proteomes of rainbow trout at different stages of gonadal development, including stages II–III (developing) and stage V (spawning). It was revealed that the brain proteomes differed in terms of proteins involved in lipid metabolism, immune responses, stress response, cell structure, and oocyte development. These findings provide valuable insights into the complex processes that occur during fish maturation and can contribute to our understanding of reproductive biology. The biochemical differences in the brain at different stages of development are shown, as well as the fact that the by-products of commercial fishing, in this case, heads and brains, can be used to extract antimicrobial proteins and peptides.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101669"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145454227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"20-Hydroxyecdysone promotes fat body lipolysis through autophagy during pupation in Apis mellifera","authors":"Jing Yu ,&nbsp;Hongfang Wang ,&nbsp;Zhenguo Liu ,&nbsp;Baohua Xu","doi":"10.1016/j.cbd.2025.101663","DOIUrl":"10.1016/j.cbd.2025.101663","url":null,"abstract":"<div><div>20-hydroxyecdysone (20E) plays a crucial role in insect metamorphosis and has been reported to be involved in fat body lipolysis during pupation in many insects, although this has not been reported in honeybees. Here, we investigated how 20E regulates lipid metabolism during pupation in <em>Apis mellifera</em>. We found that 20E promotes fat body lipolysis by inducing autophagy. 20E treatment reduced fat body triglyceride (TG) content, upregulated <em>lipase-1</em> and <em>adipokinetic hormone receptor</em> (<em>akhr</em>) expression, and promoted fat body lipolysis. Our lipidomic results showed that honeybee larvae treated with 20E showed significant changes in their TG and phosphatidylcholine (PC) fatty acid levels. In conclusion, we conclude that 20E promotes pupal development by coordinating autophagy and lipid mobilization. This work reveals a novel regulatory mechanism of insect metamorphosis in honeybees and expands our understanding of steroid hormone action in holometabolous insects.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101663"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145454293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota composition and antibiotic resistance ontology landscape in Micropterus salmoides: Insights from metagenomic and metabolomic analyses","authors":"Gang Zhou ,&nbsp;Ying-Si Wang ,&nbsp;Gui-Fang Zhang,&nbsp;Shu-Yao Zhang,&nbsp;Xia Wen,&nbsp;Zong-Bin Cui,&nbsp;Qing-Shan Shi,&nbsp;Xiao-Bao Xie","doi":"10.1016/j.cbd.2025.101666","DOIUrl":"10.1016/j.cbd.2025.101666","url":null,"abstract":"<div><div><em>Micropterus salmoides</em>, a pivotal aquaculture species in China, faces critical challenges including high disease susceptibility and insufficient characterization of gut microbiota-associated antibiotic resistance ontology (ARO). This study integrated metagenomic and metabolomic approaches to systematically characterize the compositional dynamics, diversity patterns, and spatiotemporal distribution of gut microbiota and AROs in <em>M. salmoides</em> across four developmental stages, while clarifying their interactions with metabolic pathways. Metagenomic profiling identified Proteobacteria, Firmicutes, and Fusobacteria as the dominant bacterial phyla, with <em>Acinetobacter baumannii</em> and <em>Alcanivorax profundi</em> exhibiting stage-specific abundance patterns. A total of 150 distinct ARO subtypes were identified, among which tetracycline- and glycopeptide-resistance genes (e.g., <em>tetA</em> and <em>vanR</em>) showing high abundance, with their resistance primarily mediated by efflux-driven mechanisms. Untargeted metabolomics uncovered 4459 metabolites, with robust correlations observed between core microbial genera (e.g., <em>Flavobacterium</em> and <em>Herbaspirillum</em>) and lipid/amino acid metabolic pathways. Co-occurrence network analysis further demonstrated significant interconnections between ARO subtypes and lineages of Proteobacteria/Firmicutes. Our multi-omics framework provides comprehensive insights into the gut microbiota-ARO-metabolism nexus in <em>M. salmoides</em>, thereby establishing a correlative framework for developing precision interventions to control the dissemination of antimicrobial resistance and improve disease management in sustainable aquaculture systems.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101666"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145454252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The sex-specific transcriptomic patterns in hypothalamic-pituitary-gonadal axis of Dabry's sturgeon (Acipenser dabryanus)","authors":"Xuetao Shi ,&nbsp;Yang Li ,&nbsp;Hongtao Huang ,&nbsp;Kan Xiao ,&nbsp;Jing Yang","doi":"10.1016/j.cbd.2025.101687","DOIUrl":"10.1016/j.cbd.2025.101687","url":null,"abstract":"<div><div>Dabry's sturgeon (<em>Acipenser dabryanus</em>) is one of the precious large fish species unique to the upper reaches of the Yangtze River in China. Investigating the molecular mechanisms that regulate the hypothalamic-pituitary-gonadal axis is crucial for the conservation of Dabry's sturgeon. In the present study, transcriptome sequencing and analysis were conducted on hypothalamus, pituitary, and gonad tissues of male and female Dabry's sturgeon. Raw sequence data were compiled and duplicate entries were eliminated, yielding 603,597 unigenes with an average length of 843 base pairs and an N50 value of 1056 bp. In the hypothalamus, the representative up-regulated pathways and genes include fatty acid biosynthesis (<em>fasn</em>, <em>acsl</em>) and fatty acid metabolism (<em>acd</em>), while down-regulated pathways and genes include neuroactive ligand-receptor interactions (<em>lhcgr</em>, <em>npffr1</em>, <em>gabrd</em>). In the pituitary, the representative up-regulated pathways and genes include neuroactive ligand-receptor interactions (<em>drd2</em>, <em>trhr</em>), while down-regulated pathways and genes are unsaturated fatty acid biosynthesis (<em>elovl6</em>). In the gonad, the representative up-regulated pathways and genes include unsaturated fatty acid biosynthesis (<em>fads2</em>), tryptophan metabolism (<em>cyp1a1</em> and <em>cyp1b1</em>), while down-regulated pathways and genes are ovarian steroid production (<em>fshr</em>, <em>igf1,</em> and <em>cyp11a</em>). These representative pathways and genes play an important role in the gonadal development of Dabry's sturgeon. These potential determinants provide a glimpse into of the molecular mechanisms of hypothalamic-pituitary-gonadal axis regulation in Dabry's sturgeon.</div></div><div><h3>Clinical trial number</h3><div>All fish handling and experimental procedures in this study have been approved by the Chinese Sturgeon Research Institute of China Three Gorges Corporation, and the Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes (Chinese Sturgeon Research Institute of China Three Gorges Corporation).</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101687"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145552370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute injury of liver in hybrid groupers (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀) under high-dose Edwardsiella ictaluri infection","authors":"Xiaomin Zheng ,&nbsp;Zhuojin He ,&nbsp;Lixin Ma,&nbsp;Linjie Zheng,&nbsp;Li Lin,&nbsp;Fei Shi","doi":"10.1016/j.cbd.2025.101692","DOIUrl":"10.1016/j.cbd.2025.101692","url":null,"abstract":"<div><div><em>Edwardsiella ictaluri</em> is a major fish pathogen that poses a serious threat to hybrid grouper in intensive aquaculture. This study evaluated the impact of <em>E. ictaluri</em> on liver health by analyzing histopathological changes, enzyme activity, and gene expression in infected hybrid grouper. Compared with the control group, infected fish exhibited markedly increased cytoplasmic vacuolation, inflammatory cell infiltration, nuclear pyknosis, and hepatocyte apoptosis. Moreover, biochemical assays revealed significantly elevated activities of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), and catalase (CAT). Transcriptomic analysis showed altered expression of genes associated with steroid hormone biosynthesis, PPAR signaling pathway, and protein digestion and absorption. Quantitative real-time polymerase chain reaction (qRT-PCR) further demonstrated increased mRNA expression of Nuclear Factor Kappa-B p65 (<em>NF-κB p65</em>), Toll-like Receptor 3 (<em>TLR-3</em>), Occludin (<em>OCLD</em>), Zonula Occludens-3 (<em>ZO-3</em>), and Fas cell surface death receptor (<em>Fas</em>). Redundancy analysis (RDA) revealed correlations of differentially expressed genes (DEGs) with cytoplasmic vacuolation and enzyme activities. Collectively, these findings reveal that <em>E. ictaluri</em> infection profoundly impairs liver health in hybrid grouper by disrupting hepatic structure, altering enzyme activity, and dysregulating pathways associated with immunity and metabolism.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101692"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145558599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vibrio parahaemolyticus infection in Manila clams (Ruditapes philippinarum): A case study of microbiome perturbation","authors":"Xianbo Qiu ,&nbsp;Yuhang Wang ,&nbsp;Tian Yu ,&nbsp;Dongdong Li ,&nbsp;Yue Tan ,&nbsp;Zhongming Huo ,&nbsp;Xiwu Yan","doi":"10.1016/j.cbd.2025.101655","DOIUrl":"10.1016/j.cbd.2025.101655","url":null,"abstract":"<div><div>The homeostasis of gill microbiota is essential for marine organisms' health, yet pathogen invasion disrupt this balance, impacting host physiology. As the farming density rises in the shellfish aquaculture industry, the Manila clam (<em>Ruditapes philippinarum</em>) has shown increased susceptibility to pathogens, with <em>Vibrio parahaemolyticus</em> representing a major economic threat. This study delves into the effects of <em>V. parahaemolyticus</em> on the gill microbiota of <em>R. philippinarum</em> (<em>Ruditapes philippinarum</em>) through histopathological analysis and 16S rRNA gene sequencing. At 72 hour post-infection (hpi), severe inflammatory reactions and gill damage were observed, accompanied by significant changes in microbial community structure. At 72 hpi, microbial diversity significantly shifted, with a decrease in Bacteroidota and Vibrionimonas, while Bradyrhizobium became dominant. By 96 hpi, Acidobacteriota and Chloroflexi increased, suggesting organic matter accumulation and redox alterations. LEfSe analysis identified Enterobacteriaceae, Streptomycetaceae, and Streptomycetales as biomarker taxa at 72 hpi. Metabolic pathways with increased abundance relative to 0 hpi included nucleoside metabolism, replication, and purine metabolism, while glycine, serine, and threonine metabolism showed a decreased abundance. Our findings illustrate that <em>V. parahaemolyticus</em> infection disrupts microbial and metabolic homeostasis in the gill of <em>R. philippinarum</em>, eliciting substantial inflammatory damage and offering insights into molecular mechanisms underlying clam susceptibility and potential mortality.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101655"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145411120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide characterization of the Hsp40 gene family in Clarias fuscus reveals their roles in thermal stress adaptation and immune modulation","authors":"Minghui Ye ,&nbsp;Dayan Zhou ,&nbsp;Lingwei Kong ,&nbsp;Yulei Zhang ,&nbsp;Chuanhao Pan ,&nbsp;Cailin Huang ,&nbsp;Guangli Li ,&nbsp;Huapu Chen ,&nbsp;Changxu Tian","doi":"10.1016/j.cbd.2025.101661","DOIUrl":"10.1016/j.cbd.2025.101661","url":null,"abstract":"<div><div>Thermal stress poses a significant challenge to fish physiology and aquaculture sustainability, necessitating an in-depth understanding of molecular responses to temperature fluctuations. This study characterizes the Hsp40 gene family in Hong Kong catfish (<em>Clarias fuscus</em>), a species known for its thermal resilience, to elucidate their roles in proteostasis and immune modulation. A total of 55 <em>Hsp40</em> genes were identified and categorized into three subfamilies: DnaJa (<em>n</em> = 6), DnaJb (<em>n</em> = 16), and DnaJc (<em>n</em> = 33). These genes exhibit conserved J-domains and diverse motifs, indicating adaptations to aquatic stressors. Phylogenetic analysis reveals that the Hsp40 gene family in <em>C. fuscus</em> exhibits the closest evolutionary relationship to <em>Ictalurus punctatus</em>, followed by <em>Danio rerio</em>, with distinct clustering patterns across the subtypes. Collinearity and synteny analyses indicated substantial genomic conservation of <em>Hsp40</em> genes between <em>C. fuscus</em>, <em>I. punctatus</em>, and <em>D. rerio,</em> with evidence of gene duplication and homologous relationships. The subcellular localization of Hsp40 proteins was observed in the cytoplasm, endoplasmic reticulum, mitochondria, nucleus, and extracellular regions, supporting their roles in chaperone activity and immune response regulation. Protein interaction networks identified two major clusters: one associated with protein folding and another with Hsp90-dependent immune signaling. Molecular docking confirmed stable interactions between Hsp40 and Hsp70, aiding in the mitigation of thermal-induced protein misfolding. Transcriptomic analysis under thermal stress showed significant differential expression of 30 <em>Hsp40</em> genes, with <em>dnajb11</em> and <em>dnajc5.1</em> notably differentially expressed in the liver, gills, and kidneys. Under normal-temperature conditions, 10, 6, and 12 genes were differentially expressed in the liver, gills, and kidneys, respectively. High-temperature acclimation significantly increased the number of differentially expressed genes in the kidneys (20 genes), indicating enhanced thermal tolerance. These genes bolster immunity by stabilizing proteins and modulating inflammatory pathways. These findings suggest that key Hsp40s, such as <em>dnajb11</em> and <em>dnajc5.1</em>, serve as biomarkers for selective breeding and support the development of strategies like immunomodulatory feed additives to enhance thermal resilience in <em>C. fuscus</em> aquaculture.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101661"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145369759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liver transcriptome analysis reveals genes and pathways involved in the ovarian development in spotted scat (Scatophagus argus)","authors":"Mei-Zhen Zhang ,&nbsp;Jing Zheng ,&nbsp;Li-Ting Cai ,&nbsp;Wan-Xin Li ,&nbsp;Zhi-Yuan Li ,&nbsp;Gang Shi ,&nbsp;Hong-Juan Shi ,&nbsp;Hua-Pu Chen ,&nbsp;Si-Ping Deng ,&nbsp;Yu-Cong Hong ,&nbsp;Guang-Li Li ,&nbsp;Dong-Neng Jiang","doi":"10.1016/j.cbd.2025.101550","DOIUrl":"10.1016/j.cbd.2025.101550","url":null,"abstract":"<div><div><em>Scatophagus argus</em> is a highly valuable aquaculture fish. Its artificial breeding faces problems in the induction of high quality eggs, thus necessitating studies on the regulation of ovarian development. As the centre of nutrient metabolism in fish, the liver provides the material basis for ovarian development. However, the molecular mechanism of the liver in ovarian development in <em>S. argus</em> is still unclear. In this study, a transcriptome analysis of adult <em>S. argus</em> livers at different stages of ovarian development (stages II, III and IV) was performed. 410, 1025 and 1867 differentially expressed genes (DEGs) were obtained between stages II and III, stages II and IV and stages III and IV, respectively. In GO and KEGG analyses, DEGs were mostly involved in vitellogenesis and egg envelope formation (<em>e.g.</em>, <em>erα</em>, <em>erβ1</em>, <em>vtga</em>, <em>vtgb</em>, <em>vtgc</em>, <em>zp3</em>, <em>zp4a</em> and <em>zp4b</em>), lipid metabolism and energy metabolism (<em>e.g.</em>, <em>dagt1</em>, <em>dagt2</em>, <em>lpl</em>, <em>apob</em>, <em>hk1</em>, <em>acly</em>, <em>ogdh</em>, <em>pc</em>, and <em>fbp1</em>), and hormone signaling (<em>e.g.</em>, <em>lepa</em> and <em>igfbp1</em>). Additionally, genes that were significantly upregulated in the liver at stage IV of ovarian development, compared to stages II and III, were markedly enriched in steroid biosynthesis and metabolism pathways. These findings provide clues to understanding the mechanisms of liver action in teleost ovarian development.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101550"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}