Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Transcriptomics, antioxidant enzyme activities, and immune-associated parameter analysis reveal the molecular responses of rainbow trout (Oncorhynchus mykiss) to transportation stress","authors":"Yueqing Wang,&nbsp;Yujun Kang,&nbsp;Zixuan Zhong,&nbsp;Jichang Liu,&nbsp;Jinlian Wu,&nbsp;Zhe Liu","doi":"10.1016/j.cbd.2025.101455","DOIUrl":"10.1016/j.cbd.2025.101455","url":null,"abstract":"<div><div>To explore how transportation stress affects the physiology of rainbow trout, we measure antioxidant and immunity-related indicators in spleen tissues of adult fish during transportation (at 0, 2, and 6 h) and 24 h following it (r24 h). We report a significant reduction in spleen catalase activity among the 2, 6, and r24 h groups compared with the 0 h group (<em>P</em> &lt; 0.05). Levels of glutathione peroxidase activity and total antioxidant capacity, and lipid peroxide, complement C4, and immunoglobulin M contents first decreased, then increased; complement C3 content and lysozyme activity first increased, then decreased; and tumor necrosis factor-α levels were relatively stable (<em>P</em> &gt; 0.05). Transcriptome sequencing of spleen samples at 0, 6, and r24 h using Illumina HiSeq6000 identified 4419, 2733, and 4375 differentially expressed genes (DEGs) in 0 vs 6, 6 vs r24, and 0 vs r24 h comparisons, respectively. Gene ontology annotation of these DEGs revealed them to function mainly in binding and catalytic activities, and to be significantly enriched in cellular processes and biological regulation terms. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed DEGs in the 0 h vs 6 h comparison to be significantly enriched in cytokine–cytokine receptor interaction, C-type lectin receptor signaling pathway, in regulating actin cytoskeleton, lysosome (0 vs r24 h); and in the Toll-like and MAPK signaling pathways (6 vs r24 h). Several immune-related genes were identified from both the 0 h vs 6 h and 6 h vs r24 h comparisons. These findings lay a solid foundation for further research on understanding how fish respond to transportation stress at the molecular level.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101455"},"PeriodicalIF":2.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of different stages of testis development in Portunus trituberculatus","authors":"Kai-Di Ni,&nbsp;Chao-Guang Wei,&nbsp;Jun-Quan Zhu,&nbsp;Chang-Kao Mu,&nbsp;Chun-Lin Wang,&nbsp;Cong-Cong Hou","doi":"10.1016/j.cbd.2025.101453","DOIUrl":"10.1016/j.cbd.2025.101453","url":null,"abstract":"<div><div>The swimming crab (<em>Portunus trituberculatus</em>) is an important marine economic species, however its artificial breeding yield is relatively low. Currently, the main challenge faced by the swimming crab seed industry is the reliance on wild populations for seed cultivation, which results in unstable yield and quality, affecting the healthy development of the crab farming industry to some extent. The quality of germplasm resources depends on the quality of gametes, and the quality of sperm depends on the orderly genetic regulation process of spermatogenesis. Therefore, elucidating the genetic regulatory mechanisms of spermatogenesis is of great significance for improving the germplasm resources of <em>P. trituberculatus</em>. To gain a deeper understanding of this process, we conducted a comparative transcriptome study on the testis of the swimming crab at different developmental stages. This study aims to identify key genes that regulate testicular development. We performed paraffin section identification on the testicular tissue of male crabs and conducted transcriptome analysis on the testicular tissue at five different developmental stages and somatic cells. Through differential expression analysis, we screened a total of 31,788 differentially expressed genes (DEGs) from stages I to VI. Through Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, we found that these DEGs were significantly enriched in 15 pathways, including important functional pathways such as the adrenergic signaling pathway, HIF-1 signaling pathway, and TGF-β signaling pathway. GO analysis results showed that calcium ion homeostasis and cell skeleton-related activities were significantly enriched in stage II. Further protein-protein interaction network analysis revealed 68 hub genes, including 13 eukaryotic initiation factors, 6 Ras superfamily members, and 6 genes related to cell division. In addition, genes such as Actin, Myosin, and Nup50 consistently showed high expression at all developmental stages, while genes related to calcium ion homeostasis, such as CaM, significantly increased in expression during stage II. Hsp90 and apoptosis-related genes had higher expression in stage IV, while Smad4 had higher expression in stage V. These results suggest that stage II of the swimming crab sperm development may be a critical period for spermatogenesis, and stage IV may be an important period for regulating sperm quality and quantity. This study not only provides a foundation for further research on the molecular mechanisms of testicular development and spermatogenesis in the swimming crab but also offers theoretical support for improving breeding yield, which has significant practical application value.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101453"},"PeriodicalIF":2.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and metabolomic analysis reveals the causes of mass mortality in juvenile pearl oysters (Pinctada maxima)","authors":"Jinfang Liu ,&nbsp;Qin Su ,&nbsp;Chuangye Yang ,&nbsp;Junpeng Luo ,&nbsp;Ruijuan Hao ,&nbsp;Yongshan Liao ,&nbsp;Robert Mkuye ,&nbsp;Qingheng Wang ,&nbsp;Yuewen Deng","doi":"10.1016/j.cbd.2025.101454","DOIUrl":"10.1016/j.cbd.2025.101454","url":null,"abstract":"<div><div><em>Pinctada maxima</em> is a pearl oyster species producing large, high-quality marine pearls. However, juvenile mortality (shell length &lt; 5 cm) in this species adversely affects commercial pearl production. Understanding the molecular mechanism and genes related to mass mortality will help mitigate this problem. Therefore, the present study investigated the transcriptomic and metabolic differences between pearl oysters during high mortality (HM) and after this period (PD) to shed light on the causes of juvenile mass mortality. Initial analysis of biochemical parameters revealed that protease, α-amylase, and catalase activities in the hepatopancreatic tissues of pearl oysters at the HM stage were significantly lower than at the PD stage. Conversely, glutathione and lysozyme contents, and superoxide dismutase, acid phosphatase, alkaline phosphatase activities were notably higher at the HM stage than at the PD stage. Metabolomic analysis identified 98 metabolites in the adductor muscle significantly different between the two stages, which enriched glycerophospholipid metabolism, glutathione metabolism, arachidonic acid metabolism, oxidative phosphorylation, and neuroactive ligand-receptor interaction pathways. Transcriptome analysis identified 677 differentially expressed genes in the adductor muscle between these stages, which enriched neuroactive ligand-receptor interaction, glutathione metabolism, and ECM-receptor interaction pathways. Finally, an integrated analysis of the metabolome and transcriptome suggested that pearl oysters at the HM stage experience oxidative stress, activate immune-related genes, and exacerbate the low energy status. These findings on the causes of mass mortality lay a theoretical foundation for improving the survival rate of juveniles and advancing the industrialization of <em>P. maxima</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101454"},"PeriodicalIF":2.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Joint profiling of DNA methylomics and transcriptomic reveals roles of demethylation in regeneration of coelomocytes after evisceration in sea cucumber Apostichopus japonicus","authors":"Jiong Wu ,&nbsp;Mingmei Xu ,&nbsp;Chuanxin Qin ,&nbsp;Yu Guo ,&nbsp;Yinan Wang ,&nbsp;Zhenhui Wang ,&nbsp;Qiang Li","doi":"10.1016/j.cbd.2025.101456","DOIUrl":"10.1016/j.cbd.2025.101456","url":null,"abstract":"<div><div>Generally, there is a large number of deaths for sea cucumber along with evisceration after which a weakly immune state occurs because of a poor resistance against a variety of pathogens and environmental stress. The regeneration of coelomocytes plays an important role in the remodeling of the immune defense system after evisceration with the decrease of methylation modification. In this study, the whole DNA methylation of coelomocytes is detected post evisceration in <em>Apostichopus japonicus</em> to explore the process of cell regeneration. Our results found that total methylation level reached a lowest point at 12 h (9.8 %), which was followed by increased at 24 h. The transcriptomic and DNA methylomic analysis indicated a total of 215 genes were identified by selecting the significant hypomethylation within the 2-kilobase region of the transcriptional start site upstream. The KEGG pathway enrichment analyses of the 215 genes showed that signal transduction, signaling molecules and interaction were significantly enriched. The genes were enriched in the top 20 signaling pathway, such as IGF1R, Notch2 and HSPA1s. Taken together, this study provides new clues for deciphering the coelomocytes regeneration after evisceration of <em>A. japonicus</em> by DNA demethylation.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101456"},"PeriodicalIF":2.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and expression profile analysis of the Ras superfamily genes in eastern honeybee (Apis cerana)","authors":"Dufu Li ,&nbsp;Jun Zhang ,&nbsp;Siming Liu ,&nbsp;Yubo He ,&nbsp;Qiang Ma ,&nbsp;Pengfei Wang ,&nbsp;Zhengang Ma ,&nbsp;Jinshan Xu ,&nbsp;Zeyang Zhou","doi":"10.1016/j.cbd.2025.101447","DOIUrl":"10.1016/j.cbd.2025.101447","url":null,"abstract":"<div><div>The Ras superfamily, a crucial gene family in eukaryotes, functions as molecular switches that regulate various physiological and biochemical processes within cells. However, research on this topic remains limited concerning <em>Apis cerana</em>, a pollinator of significant economic and ecological importance. Here, 83 Ras superfamily genes in the genome of <em>A. cerana</em> were identified and classified into five distinct families. Phylogenetic analysis revealed that the homologous genes from two closely related species, <em>A. cerana</em> and <em>Apis mellifera</em>, clustered with relatively high bootstrap values. Notably, <em>Rerg</em> was unique to <em>A. cerana</em> compared to <em>A. mellifera</em> and had undergone gene duplication events. Expression profiles indicated diverse expression patterns of the Ras superfamily in the fat body during the holometabolous development of <em>A. cerana.</em> Most genes exhibited high expression levels during the later stages of adipose tissue remodeling; however, a minority were predominantly expressed during the prepupal (PP) and 1-day-old pupae (P1) stages. Among the 8 pairs of genes that had undergone gene duplication, 4 had inconsistent patterns, and 3 pairs were from the Ras family. In particular, <em>Di-Ras2-2</em> was found to be highly expressed exclusively at the PP, and <em>Rap2a-2</em> was only highly expressed during newly emerged (Em) stage. The observed differences in expression patterns may reflect adaptations to varying energy metabolism modes or environmental pressures. These findings provided a foundational basis for further investigation into the potential physiological roles of the Ras superfamily in <em>A. cerana</em> and offered valuable insights for analyzing this gene family across other insect species.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101447"},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome signatures of the lipid metabolism in the liver and partial characterisation of the plasma phospholipidome of a long-distance migratory bird, the Northern Wheatear (Oenanthe oenanthe)","authors":"Roberto Carlos Frias-Soler ,&nbsp;Natalie A. Wellbrock ,&nbsp;Laura Bindila ,&nbsp;Michael Wink ,&nbsp;Franz Bairlein","doi":"10.1016/j.cbd.2025.101452","DOIUrl":"10.1016/j.cbd.2025.101452","url":null,"abstract":"<div><div>The Northern Wheatear (<em>Oenanthe oenanthe</em>) is a long-distance migratory bird that has become a model species for the study of the phenomenology and molecular aspects of avian migration. Here, we analysed transcriptomic data related to the lipid metabolism in the liver of wheatears during the development and termination of the migratory fattening. In parallel, we partially characterised their plasma phospholipidome. Based on transcriptomic data, we found evidence of a fine-scale regulation of the lipogenesis/lipolysis rate and over the fatty acid composition during the migratory season. Furthermore, our results suggest a regulated production of oxylipins, signaling lipids derivatives of polyunsaturated fatty acids (PUFAs). Regarding the plasma phospholipid profiling, different lipid species showed a significant differential abundance among migratory stages: lysophosphatidylcholine (LPC 18:0), sphingomyelin (SM 34:1;O2) and phosphatidylinositols (PI 36:4 and PI 38:4). The liver transcriptomic and plasma lipidomic data agree well, showing the relevance of the liver in controlling the lipid metabolism in relation to migration. We hope that the results discussed in this publication would open the door for future functional genetic and metabolic studies regarding avian migration.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101452"},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and lipidome integration unveils key mechanisms constraining bivalve larval sensitivity in an acidifying sea","authors":"Yang Xu ,&nbsp;Fortunatus Masanja ,&nbsp;Yuewen Deng ,&nbsp;Liqiang Zhao","doi":"10.1016/j.cbd.2025.101450","DOIUrl":"10.1016/j.cbd.2025.101450","url":null,"abstract":"<div><div>The intensity, frequencye and duration of seawater acidification in coastal seas have already surpassed projections for open oceans. Bivalve larvae are extremely sensitive to intensifying coastal seawater acidificaiton during their initial shell building, a critical period constraining recruitment success and population maintenance, but underlying mechanisms of larval shell formation sensitivity to acidification remain largely debated. Here, we performed an integrated analysis of the transcriptome and lipidome of trochophore of <em>Ruditapes philippinarum</em> to compare the core molecular responses involved in initial shell formation under ambient (pH 8.1), moderately (pH 7.7), and severely (pH 7.4) acidified conditions. Ocean acidification (OA) affected the ion transport efficiency by inhibiting gene expression of key ion transporters, thereby inhibiting initial shell formation, but the gene downregulation in the moderate exposure group was more significant. OA also induced major membrane lipid remodeling in larvae, which also significantly affected the ion transport efficiency. The TAG content of larvae which sustained the energy supply for active transport of calcification substrates and synthesis of organic matrix in the severe exposure group was significantly reduced. Overall, OA inhibited the formation of the initial larval shell, but different levels of OA had different inhibitory mechanisms on the initial larval shell formation, and the present study also further identified the role of lipids in initial shell formation, which can provide a theoretical basis for for a more accurate and comprehensive assessment of the impact of OA on bivalve calcification in an acidifying ocean.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101450"},"PeriodicalIF":2.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic metabolic profiling of sea cucumbers (Apostichopus japonicus) under predation stress","authors":"Yongxin Sun ,&nbsp;Shuo Wang ,&nbsp;Chong Wang ,&nbsp;Meng Wang ,&nbsp;Liang Qu ,&nbsp;Jian Song ,&nbsp;Chong Zhao ,&nbsp;Qingzhi Wang","doi":"10.1016/j.cbd.2025.101451","DOIUrl":"10.1016/j.cbd.2025.101451","url":null,"abstract":"<div><div>The present study utilized non-targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) to investigate the metabolomic responses of sea cucumber (<em>Apostichopus japonicus</em>) juveniles under predation stress induced by sea stars (<em>Asterina pectinifera</em>) at various time points (3 h, 12 h, 72 h, and 96 h). The findings revealed significant temporal changes in the metabolic profiles of the sea cucumber juveniles under predation stress, with 25, 72, 55, and 53 metabolic products exhibiting significantly different expression levels at each time point (positive ion mode, <em>P</em> &lt; 0.05), respectively. Notably, the impact of predation stress was most pronounced at the 12-h mark. Multivariate statistical methods, including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), further confirmed distinct clustering of the experimental group away from the control group at each time point, with the most pronounced separation occurring at 12 h, indicating a significant and time-dependent metabolic response to predation stress. Key metabolic pathways associated with predation stress were identified, such as carbon metabolism, pentose phosphate pathway, purine metabolism, riboflavin metabolism, longevity regulation, and antifolate resistance pathways, by integrating variable importance in the projection (VIP), fold change (FC), and <em>P</em>-value. KEGG enrichment analysis highlighted significant expression changes of key metabolites like carbamoyl phosphate, gluconolactone, inosine, 2′-deoxyguanosine, and adenylate in response to predation stress, potentially related to energy metabolism, antioxidant defense, signal transduction, and cellular stress responses. The study provides novel insights into the metabolic adaptability of sea cucumber juveniles to predation stress.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101451"},"PeriodicalIF":2.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-negative matrix factorization reveals seasonal and sex-specific metabolic tactics in tropical tilapia","authors":"Min-Chen Wang ,&nbsp;Ching-Wei Wang ,&nbsp;Fumiya Furukawa ,&nbsp;Ching-Chun Lin ,&nbsp;Tzu-Hao Lin ,&nbsp;Yung-Che Tseng","doi":"10.1016/j.cbd.2025.101448","DOIUrl":"10.1016/j.cbd.2025.101448","url":null,"abstract":"<div><div>Understanding metabolic adaptations to seasonal fluctuations in ectothermic organisms is challenging, especially in tropical species where physiological responses are more pronounced than in temperate or polar counterparts. Traditional analytical methods often fail to account for the complex metabolic adjustments that are present in these substantial responses, and the high-dimensional characteristics of metabolomic data complicate the interpretation process when using conventional statistical methods. We utilized Non-negative Matrix Factorization (NMF), an unsupervised machine learning algorithm, to analyze monthly serum metabolomics data from tilapia over a year in order to overcome these limits. A deeper analysis using NMF demonstrated that carbohydrates gained prominence during warmer months, as evidenced by consistently elevated weights of glycolysis intermediates in our quantitative analysis. Furthermore, fatty acids remained an important factor in both warm and cold seasons. Amino acids emerged as particularly versatile metabolites, exhibiting adaptability during seasonal transitions. This flexibility suggests their crucial role in coordinating energy-related adaptations and potentially facilitating epigenetic and reproductive responses to changing environments. Serum aspartate composition during the warm-cold transition indicated sex-specific metabolic strategies, as sexual dimorphism was observed in the seasonal utilization of fatty acids and aspartate. Collectively, NMF objectively assesses the metabolic tactics of tropical fish and reveals latent patterns in real-world metabolic dynamics. Consequently, it possesses the potential to facilitate metabolomics-driven species conservation in response to environmental changes.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101448"},"PeriodicalIF":2.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of the spermatogonial stem cell line of Opsariichthys bidens after infection with three strains of ranaviruses","authors":"Lang Gui ,&nbsp;Shiwen Zhang ,&nbsp;Zhongyi Liu ,&nbsp;Linwen Cheng ,&nbsp;Qiqi Jiang ,&nbsp;Mingyou Li ,&nbsp;Dong Liu ,&nbsp;Jianfeng Ren ,&nbsp;Fei Ke","doi":"10.1016/j.cbd.2025.101438","DOIUrl":"10.1016/j.cbd.2025.101438","url":null,"abstract":"<div><div>The long-term-cultured <em>Opsariichthys bidens</em> spermatogonial stem cell line (ObSSC) was established in our previous study, which could be induced to differentiate into sperm <em>in vitro</em>. The genus <em>Ranavirus</em> is a large double-stranded DNA virus with a high degree of pathogenicity, which can be widely transmitted in fish, amphibians and reptiles. It is highly representative and possesses significant research value. The present study aimed to investigate the morphological changes and transcriptome analyses of ObSSC following infection by the three strains of ranaviruses (<em>Andrias davidianus</em> ranavirus, ADRV; <em>Rana grylio</em> virus, RGV; <em>Siniperca chuatsi</em> ranavirus, SCRaV). Microscopic observation and polymerase chain reaction (PCR) detection techniques were employed to ascertain that ObSSC could be invaded by ranaviruses, resulting in the formation of cell shed plaques and other lesions. Transcriptome analysis revealed the high degree of similarity among different infections of ranaviruses. The genes <em>Egr1</em> (early growth response protein 1) and <em>Dusp2</em> (dual specificity protein phosphatase 2) were downregulated after infection. The downregulation of PI3K-AKT and MAPK signaling pathways was observed in all infected groups, which may be related to the interference of viral infection on cell growth, biological process and apoptosis. At the same time, specific responses of the host cells were observed by infections of different types of ranaviruses. The expression of phagosome was upregulated in the ADRV-infected group, whereas the Junctional Adhesion Molecule (JAM2) and the leukocyte transendothelial migration pathway was upregulated in the RGV-infected group, and the Fc gamma R-mediated phagocytosis was upregulated in the SCRaV-infected group. These findings provided important clues for further understanding of the mechanism of ranaviruses infection and host cell response.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101438"},"PeriodicalIF":2.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}