Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Genomics and transcriptomics identify quantitative trait loci affecting growth-related traits in silver pomfret (Pampus argenteus)","authors":"Cheng Zhang ,&nbsp;Shun Zhang ,&nbsp;Guanlin Wang ,&nbsp;Xiang Huang ,&nbsp;Shanliang Xu ,&nbsp;Danli Wang ,&nbsp;Chunyang Guo ,&nbsp;Yajun Wang","doi":"10.1016/j.cbd.2025.101414","DOIUrl":"10.1016/j.cbd.2025.101414","url":null,"abstract":"<div><div><em>Pampus argenteus</em>, a species distributed throughout the Indo-West Pacific, plays a significant role in the yield of aquaculture species. However, cultured <em>P. argenteus</em> has always been characterised by unbalanced growth synchronisation among individuals, slow growth rate, and lack of excellent germplasm resources. Therefore, we conducted mass selection for fast-growing strain <em>P. argenteus</em> for several consecutive years. Various genetic improvement programs have modified its genome sequence through selective pressure, leaving nucleotide signals that can be detected at the genomic level. In the present study, we combined bulked segregant analysis and transcriptome sequencing to identify candidate single nucleotide polymorphisms (SNPs) and key genes for growth-related traits in <em>P. argenteus</em>. A total of 7,280,936 SNPs and 2,212,379 insertions/deletions were identified in the extreme phenotypes of the fast-growing and slow-growing groups. Based on the examination of SNP frequency differences and sliding-window analysis, 42 SNPs were identified as candidate markers. Moreover, 14 of the 42 SNPs linked to growth-related traits were confirmed to be credible SNPs, and eight growth-related genes were screened, namely myb-binding protein 1 A, insulin A/B chains, α-1B adrenoceptor, engulfment and cell motility protein 3, myosin light chain kinase family member 4, insulin receptor located, unconventional myosin-9b, and matrilin-1. An optimal three-factor model (SNP4&amp;SNP12&amp;SNP14) was constructed using the generalized multifactor dimensionality reduction method, and its accuracy was verified as 67.72 %. These results may benefit genetic studies and accelerate genetic improvement of fast-growing strains of <em>P. argenteus</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101414"},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis reveals the DNA replication genes response to Vibrio anguillarum and NNV infection in Jinhu grouper (Epinephelus fuscoguttatus♀ × Epinephelus tukulal♂)","authors":"Huimin Duan ,&nbsp;Yazhuo Zhang ,&nbsp;Jia Liu ,&nbsp;Gehui Ren ,&nbsp;Zhentong Li ,&nbsp;Yongsheng Tian","doi":"10.1016/j.cbd.2025.101421","DOIUrl":"10.1016/j.cbd.2025.101421","url":null,"abstract":"<div><div><em>Vibrio anguillarum</em> acts as an infectious agent in the aquaculture industry that causes a fatal hemolytic septicaemic disease in fish and shellfish. Viral nervous necrosis (VNN) disease seriously impacts the healthy development of the aquaculture industry. While the detrimental effects of <em>V. anguillarum</em> and NNV have been widely researched on freshwater and marine fish, whether they affect DNA replication in fish is unclear. In this study, we used Jinhu grouper as a model to investigate the influence of <em>V. anguillarum</em> and NNV on their DNA replication. By RNA-seq analysis in conjunction with gene set enrichment analysis, we found a significant upregulation of genes related to DNA replication in the liver and spleen of the Jinhu grouper after <em>V. anguillarum</em> infection while a prominent downregulation of genes related to DNA replication in the brain of the Jinhu grouper after NNV infection. We identified 27, 29 and 18 key genes involved in DNA replication that may respond to <em>V. anguillarum</em> and NNV infection and selected and identified six DNA replication genes (<em>pole</em>, <em>pole2</em>, <em>pold1</em>, <em>pola1</em>, <em>pcna</em> and <em>mcm2</em>). In addition, our results indicated that these genes exhibit tissue-specific expression patterns, with an increasing pattern of expression when <em>V. anguillarum</em> infected and a decreasing pattern when NNV infected. These results may provide valuable understanding on the underlying mechanisms of <em>V. anguillarum</em> and NNV infection in fish.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101421"},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MeRIP-seq data analysis and validation reveal the regulatory role of m6A modified circRNAs in the apoptosis of secondary hair follicle cells in Inner Mongolia cashmere goats","authors":"Ru Zhang ,&nbsp;Jiayue Liang ,&nbsp;Zhaomin Liu ,&nbsp;Jiale Chang ,&nbsp;Junjie Zhang ,&nbsp;Zhanxiong Zhang ,&nbsp;Rui Li ,&nbsp;Wei Zhao ,&nbsp;Yongyuan Deng ,&nbsp;Hongmei Xiao","doi":"10.1016/j.cbd.2025.101419","DOIUrl":"10.1016/j.cbd.2025.101419","url":null,"abstract":"<div><div>As a widely epigenetic modification, m⁶A (N⁶-methyladenosine, m⁶A) can regulate the degradation, translation, and other biological functions of circRNAs through dynamic reversible processes. It plays an important role in regulating the life activities of biological organisms, particularly in cell differentiation, apoptosis, embryonic development, stress response, and innate immunity. In this study, bioinformatics analysis, qRT-PCR identification, FISH subcellular localization, and ceRNA network construction were performed on m⁶A modified circRNAs regulating the apoptosis of secondary hair follicle cells of Inner Mongolia Albas white cashmere goats based on the skin m⁶A sequencing data of secondary hair follicles in anagen and catagen. The results showed that 8 m⁶A modified circRNAs regulating the cell apoptosis of secondary hair follicles, namely circRNA_2130, circRNA_0013, circRNA_1203, circRNA_1462, circRNA_1242, circRNA_2308, circRNA_2654 and circRNA_1442 were identified, and they are respectively derived from <em>ANGEL2</em>, <em>APP</em>, <em>GKAP1</em>, <em>HNRNPC</em>, <em>PTBP3</em>, <em>NUCB1</em>, <em>SNRK</em> and <em>ZNF609</em> genes. Among them, circRNA_0013, circRNA_1442 and circRNA_1462 were located in the cytoplasm of the secondary hair follicle papilla, while circRNA_1203, circRNA_1242, circRNA_2130, circRNA_2308 and circRNA_2654 were located in the nucleus. There are complex and diverse regulatory relationships among 8 circRNAs, with each circRNA targeting one or more miRNAs, revealing that each m⁶A circRNA can exert regulatory effects through multiple potential miRNA-mRNA axes, to regulate the apoptosis of secondary hair follicle cells of cashmere goats during the growth cycles. This result provides a direction for further elucidating the regulatory mechanism of m⁶A modified circRNAs in cashmere growth and exploring biomarkers.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101419"},"PeriodicalIF":2.2,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analyses of glutathione S-transferase gene family and expression profiling among three haplotypes Aphis gossypii","authors":"Yaling Zhang ,&nbsp;Muhammad Farhan ,&nbsp;Hanjing Yang ,&nbsp;Jun Zhao ,&nbsp;Xiaoyan Ma ,&nbsp;Shuai Zhang","doi":"10.1016/j.cbd.2025.101416","DOIUrl":"10.1016/j.cbd.2025.101416","url":null,"abstract":"<div><div>Glutathione S-transferase (GST) plays a critical role in detoxifying various chemical compounds and is essential for host adaptation and pesticide resistance in insects. To understand the genetic structure of the GST family and the expression patterns among three haplotypes of <em>Aphis gossypii</em>, we conducted studies using genome annotation files and RNA-seq data. We identified 11 <em>GSTs</em> in <em>A. gossypii</em>, which include 2 microsomal <em>GSTs</em>, 2 in the Theta class, 2 in the Delta class, 3 in the Sigma class (one of which contains a probable pseudogene), 1 in the Omega class, and 1 belonging to a GST C-terminal domain-containing protein. These <em>GSTs</em> are distributed on autosomes, with the two Delta class GSTs and two Theta class GSTs clustered on a chromosome. <em>AgosGSTCD3</em> is evolutionarily conserved in aphids, encoding 550 amino acids with the C-terminal region containing a methyltransferase domain. <em>AgosGSTo4</em> exhibits alternative splicing, resulting in the formation of three isoforms. <em>GSTs</em> show conservation among Hap1, Hap3, and Hap4, except for <em>AgosGSTm9</em>, which demonstrates noticeable variations in the protein-coding regions and surrounding areas among the three haplotypes of <em>A. gossypii</em>. The expression profiles of <em>GSTs</em> were observed in the three haplotypes of <em>A. gossypii</em>, most showing different expression levels. These findings provide a valuable resource for further investigations into population differentiation and pesticide resistance evolution.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101416"},"PeriodicalIF":2.2,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of long non-coding RNAs and their multiple regulation mechanism in shell deposition of pearl oyster","authors":"Minxin Liang ,&nbsp;Jinping Xie ,&nbsp;Yiwei Huang ,&nbsp;Qingheng Wang ,&nbsp;Ronglian Huang ,&nbsp;Yongshan Liao ,&nbsp;Yuewen Deng ,&nbsp;Chuangye Yang ,&nbsp;Zhe Zheng","doi":"10.1016/j.cbd.2025.101415","DOIUrl":"10.1016/j.cbd.2025.101415","url":null,"abstract":"<div><div>Biomineralization to fabricate diverse morphology shell is typical character of bivalve species and ectopic calcification to form is the production of defense. Long non-coding RNAs (LncRNAs) plays critical roles in multiple cellular biological processes in invertebrate and vertebrate. However, LncRNAs remain poorly understood about expression and regulation roles in bivalve biomineralization studies. In this research, we systematically identified lncRNAs that functioned in differentiated mantle regions for fabricating two phenotypes of CaCO₃ crystal shell, along with pearl sac depositing pearls for ectopic calcification by CaCO₃. A total of 48,555 non-coding transcripts were obtained. 229 differentially expressed lncRNAs in mantle tissue as well as 401 differentially expressed lncRNAs in the comparison of mantle center to pearl sac were identified. By cis- and trans-regulation ways, these DELs could be involved in provisioning and metabolism such as chitin, glycosaminoglycan, ECM/shell matrix protein, Ca²⁺ and HCO₃⁻ offering, and also affecting related signaling pathways. In addition, lncRNAs were found to crosstalk with miRNAs not only as primary competing endogenous RNAs but as the primary miRNAs. Our studies provided insight into non-coding RNA regulation of epigenetic regulation in biomineralization in bivalve species.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101415"},"PeriodicalIF":2.2,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of microgastropoda species provides novel insights into phylogeny of Trochoidea (Gastropoda: Vetigastropoda)","authors":"Jihang Gong ,&nbsp;Xiao Han ,&nbsp;Lu Qi ,&nbsp;Lingfeng Kong ,&nbsp;Qi Li","doi":"10.1016/j.cbd.2025.101420","DOIUrl":"10.1016/j.cbd.2025.101420","url":null,"abstract":"<div><div>Trochoidea is the richest and most diverse group within Vetigastropoda, serving as one of the main focuses on studies of marine ecology and systematics. Both morphological and molecular studies have sought to resolve the phylogenetic framework of Trochoidea; however, the phylogenetic relationships among some lineages remain controversial. In order to explore the phylogenetic relationships within Trochoidea, we sequenced the mitochondrial genomes of 9 trochoids and analyzed them with data from 38 previously published mitochondrial genomes and 27 transcriptomic data representing 11 families within this group. The mitochondrial genomes of all Trochidae exhibited a consistent gene arrangement and showed conserved genome size and nucleotide composition; however, Colloniidae and Phasianellidae showed higher levels of gene order rearrangement. Furthermore, three-nucleotide insertions were observed in the <em>cox1</em> gene of Colloniidae and <em>nad4L</em> gene of Phasianellidae, while three-nucleotide deletions were detected in <em>nad4</em> gene of Trochidae. Both maximum likelihood and Bayesian inference analyses supported the monophyly of all families within the Trochoidea, except Tegulidae, and placed Liotiidae as sister to the rest of Trochoidea with poor to moderate support. Areneidae was recovered as the sister group to a clade including Phasianellidae and Colloniidae. These findings challenge the traditional classification of this family based on both morphological and molecular data. Our study provides new insights into the phylogeny of Trochoidea, especially with the incorporation of micromolluscs taxa, and highlight the significance of incorporating microgastropoda taxa into molecular phylogenetic reconstructions of gastropod subgroups.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101420"},"PeriodicalIF":2.2,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptomic analysis of left-right sensory differences in Haliotis discus hannai","authors":"Haifeng Huang ,&nbsp;Lintao Tan ,&nbsp;Lei Wei ,&nbsp;Hongce Song ,&nbsp;Wenwen Xu ,&nbsp;Meiyun Dong ,&nbsp;Xiaolong Chu ,&nbsp;Xiaotong Wang","doi":"10.1016/j.cbd.2025.101417","DOIUrl":"10.1016/j.cbd.2025.101417","url":null,"abstract":"<div><div>Asymmetric development, in which functional differences occur between left-right symmetrical organs, is widespread in organisms, including fish and mollusks. However, the asymmetry of symmetrical sensory structures in <em>Haliotis discus hannai</em>, a gastropod with a sensitive sensory system, remains unknown. This study analyzed the transcriptomes of three sensory structures (eyestalks, cephalic tentacles, and epipodial tentacles) to explore potential asymmetries in this species. RNA-seq revealed functional differences in sensory ability and sperm-egg recognition between right and left eyestalks, with cephalic tentacles displaying asymmetry in cytoskeletal organization and cell cycle regulation. Epipodial tentacles showed similar asymmetries, including immune response differences. Moreover, the cAMP-protein kinase A (<em>PKA</em>)-CREB-binding protein (<em>CBP</em>) signaling pathway responded asymmetrically, with <em>PKA</em> responding to activators and inhibitors on both sides and <em>CBP</em> showing a stronger response on the right. These findings provide insights into sensory asymmetry in mollusks and guidance for further investigations of the molecular mechanisms underlying asymmetry in symmetrical organs.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101417"},"PeriodicalIF":2.2,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide characterization of the TRP gene family and transcriptional expression profiles under different temperatures in gecko Hemiphyllodactylus yunnanensis","authors":"Chao Li ,&nbsp;Xiaoying Liu ,&nbsp;Chaochao Hu,&nbsp;Jie Yan,&nbsp;Yanfu Qu,&nbsp;Hong Li,&nbsp;Kaiya Zhou,&nbsp;Peng Li","doi":"10.1016/j.cbd.2025.101418","DOIUrl":"10.1016/j.cbd.2025.101418","url":null,"abstract":"<div><div>Temperature is closely linked to the life history of organisms, and thus thermoception is an important sensory mechanism. Transient receptor potential (TRP) ion channels are the key mediators of thermal sensation. In this study, we analyzed the sequence characteristics of TRPs in gecko <em>Hemiphyllodactylus yunnanensis</em> and compared the phylogenetic relationships of TRP family members among different Squamata species. In addition, we sequenced the transcriptome of skin and brain tissues of <em>H. yunnanensis</em> exposed to 12 °C (cold), 20 °C (cool), 28 °C (warm), and 36 °C (hot). The results showed that a total of 591 <em>TRPs</em> were identified in the genomes of 21 Squamate species, and these genes were classified into six subfamilies. Among them, 26 <em>TRP</em> genes were identified in <em>H. yunnanensis</em> and distributed on 13 chromosomes. Overall, <em>TRP</em> genes were conserved in squamates. Based on the transcriptome results, we found a total of 9 <em>TRP</em> genes expressed in the brain and skin of <em>H. yunnanensis</em>, of which six <em>TRP</em> genes were under positive selection. <em>TRPP1L2</em>, <em>TRPP1L3</em>, and <em>TRPV1</em> were involved in heat-sensitive responses (&gt; 36 °C), and <em>TRPV3</em>, <em>TRPA1</em>, and <em>TRPM8</em> were involved in cold-sensitive responses (&lt; 20 °C). TRPM8 and TRPP1L2 were important cold and heat sensors in <em>H. yunnanensis</em>, respectively.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101418"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of triclocarban exposure on development and gene expression in the wolf spider, Pardosa pseudoannulata (Araneae: Lycosidae)","authors":"Fengjie Liu ,&nbsp;Wei Li ,&nbsp;Yanrong Wu ,&nbsp;Zhilin Chen ,&nbsp;Xingyang Zhao ,&nbsp;Jie Liu ,&nbsp;Yu Peng ,&nbsp;Yao Zhao ,&nbsp;Yang Zhu","doi":"10.1016/j.cbd.2025.101413","DOIUrl":"10.1016/j.cbd.2025.101413","url":null,"abstract":"<div><div>Triclocarban (TCC), an emerging organic contaminant, poses a potential threat to water resources and ecosystems. The wolf spider <em>Pardosa pseudoannulata</em> (Araneae: Lycosidae) is a dominant predator typically inhabiting rice fields or wet habitats near water sources. However, little is known about the effects of TCC on the wolf spiders. In the present study, using environmentally relevant concentrations, we systematically investigated the toxicity of TCC on the early life stages of <em>P. pseudoannulata</em> at both physiological and gene expression levels. Our results indicated that TCC exposure did not significantly affect the survival and development of spiderlings. Transcriptome analysis revealed that TCC stress significantly impacted drug metabolism, cell cycle and signal transduction pathways in spiderlings. Subsequently, qRT-PCR was used to verify the expression levels of genes associated with drug metabolism and cell process, and the results were consistent with the transcriptome. These findings enhance our understanding of the toxic mechanism and ecological risk of TCC on arthropods.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101413"},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional verification of a landmark gene EVM713 involved in spermatogenesis in the marine bivalve Chlamys nobilis","authors":"Xixi Duan ,&nbsp;Mingfei Feng ,&nbsp;Xiangdong Bai ,&nbsp;Imran Rashid Rajput ,&nbsp;Hongkuan Zhang ,&nbsp;Huaiping Zheng","doi":"10.1016/j.cbd.2024.101412","DOIUrl":"10.1016/j.cbd.2024.101412","url":null,"abstract":"<div><div>The formation of broodstock gametes is closely linked to the yield and quality in aquaculture production, yet molecular mechanisms underlying this process remain insufficiently understood. The noble scallop <em>Chlamys nobilis</em>, an economically significant dioecious bivalve species, serves as an excellent model for studying gametogenesis. In this study, the adult scallops with testis at different developmental stage were chosen for histological examination and transcriptome analysis to dig genes related gonad development. Totally, 2663 DEGs and their set modules significantly related to spermatogenesis were obtained using WGCNA, including 40 candidate genes represented by <em>EVM713</em>. The gene was specifically expressed in the testis. RNA interference (RNAi) of <em>EVM713</em> led to impaired testis development, marked by sparse sperm cell arrangement, spermatocytes detaching from the follicle wall, and reduced spermatocyte numbers. Meanwhile, 24 h after RNAi, the expression levels of <em>Bax</em>, and <em>Caspase3</em> significantly increased (<em>P</em> &lt; 0.05), while those of <em>Bcl2</em>, <em>Dmrt2</em> and <em>Tssk4</em> were significantly decreased (<em>P</em> &lt; 0.05). These results indicate that <em>EVM713</em> is essential for spermatogenesis in bivalves, regulating testis development through the modulation of <em>Dmrt2</em> and <em>Tssk4</em> expression. This study provides the first evidence of <em>EVM713</em> function in mollusks, which is conducive to better understanding molecular mechanisms underlying gametogenesis in marine invertebrates.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"54 ","pages":"Article 101412"},"PeriodicalIF":2.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}