Functional characterization of a novel FpVgR: A special focus on ovarian development in Fenneropenaeus penicillatus

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-03-17 DOI:10.1016/j.cbd.2025.101478

Yaxin Dong , Karsoon Tan , Xueyu Yan , Boyu Su , Leong-Seng Lim , Khor Waiho , Kit Yue Kwan , Kianann Tan

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Abstract

Redtail shrimp Fenneropenaeus penicillatus are commercially important shrimp species. However, the aquaculture of this species is hindered by insufficient seed supply, mainly caused by frequent inadequate gonad development. The vitellogenin receptor (VgR) plays an important role in the gonad development of oviparous animals by facilitating the accumulation of vitellogenin and nutrients in the oocytes. Therefore, in this study, we cloned and functionally characterized a novel VgR from F. penicillatus (FpVgR). In general, FpVgR has a length of 3795 bp, encoding 1264 amino acid residues and encompassing 28 exons. The calculated molecular weight and theoretical isoelectric point of FpVgR were 139.18 kDa and 4.76, respectively. FpVgR mRNA was highly expressed in the ovary at developmental stages 3 and 4, and localized in the oocyte's plasma membrane. Knocking down FpVgR significantly reduced transcription levels in ovarian tissue, resulting in DNA damage and cell apoptosis within the ovarian tissues. The results of transcriptomic profiling following FpVgR knockdown also revealed that the apoptosis signaling pathway and oxytocin signaling pathway were involved in regulating ovary development and maintaining homeostasis. These findings offer valuable understanding into the mechanisms governing vitellogenesis and the maturation of oocytes, with a specific focus on FpVgR, contributing to future research on vitellogenesis and ovarian development in F. penicillatus.

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一种新型FpVgR的功能表征：特别关注青霉对虾卵巢发育。

红尾虾是具有重要商业价值的虾种。然而，该物种的养殖受到种子供应不足的阻碍，主要是由于性腺发育经常不足。卵黄原蛋白受体（vitellogenin receptor, VgR）通过促进卵黄原蛋白和营养物质在卵母细胞内的积累，在卵生动物的性腺发育中起着重要作用。因此，在本研究中，我们克隆了一种新的青霉菌VgR (FpVgR)，并对其进行了功能表征。一般来说，FpVgR的长度为3795 bp，编码1264个氨基酸残基，包含28个外显子。FpVgR的计算分子量为139.18 kDa，理论等电点为4.76。FpVgR mRNA在卵巢发育第3、4期高表达，定位于卵母细胞的质膜。抑制FpVgR显著降低卵巢组织的转录水平，导致卵巢组织内DNA损伤和细胞凋亡。FpVgR敲低后的转录组分析结果也揭示了凋亡信号通路和催产素信号通路参与调节卵巢发育和维持体内平衡。这些发现对卵黄形成和卵母细胞成熟的机制提供了有价值的理解，特别是对FpVgR的关注，有助于进一步研究青霉菌的卵黄形成和卵巢发育。

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.