The immune response and autophagy of Macrobrachium rosenbergii against Aeromonas veronii infection

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-03-18 DOI:10.1016/j.cbd.2025.101488

Liying Zhou, Xiaoyu Cai, Qi Dong, Jia Yin, Yan Liu, Xiaojian Gao, Qun Jiang, Yao Zhang, Xiaojun Zhang

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引用次数: 0

Abstract

Aeromonas is a bacterial pathogen that causes significant economic losses in the Macrobrachium rosenbergii industry. This study evaluated the transcriptome analysis of M. rosenbergii infected with A. veronii and examined the gene expression patterns associated with immunity in the gills, muscles, intestines, and hepatopancreas. Specifically, 47,988 unigenes and 15,604 differentially expressed genes (DEGs) were identified. The immune-related DEGs were primarily enriched in 20 innate immune signaling pathways, including the NOD-like receptor, Toll-like receptor, and RIG-I-like receptor signaling pathways, etc., as determined by KEGG enrichment analysis. Notably, autophagy-related genes ATG5, ATG12, ATG16L1, and ATG8 were enriched in the NOD-like receptor signaling pathways. Moreover, ATG12, ATG16L1, and ATG8 exhibited significantly up-regulated expression to varying degrees in the hepatopancreas, gills, muscles, and intestines at 12, 24, 36, and 48 h post-infection (hpi). In addition, many autolysosomes were observed in hepatopancreas cells of infected prawns using transmission electron microscopy (TEM). Ultimately, we identified the autophagy-related genes implicated in the immune response of M. rosenbergii, offering a theoretical foundation for elucidating the role of autophagy in the prawn's innate immune mechanisms.

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罗氏沼虾对维罗氏气单胞菌感染的免疫反应和自噬

气单胞菌是造成罗氏沼虾工业重大经济损失的一种细菌病原体。本研究评估了罗氏微孢子虫感染罗氏微孢子虫的转录组分析，并检测了鳃、肌肉、肠和肝胰腺中与免疫相关的基因表达模式。具体来说，共鉴定出47,988个单基因和15,604个差异表达基因（deg）。通过KEGG富集分析，免疫相关的deg主要富集在20条先天免疫信号通路中，包括nod样受体、toll样受体、rig - i样受体信号通路等。值得注意的是，自噬相关基因ATG5、ATG12、ATG16L1和ATG8在nod样受体信号通路中富集。此外，ATG12、ATG16L1和ATG8在感染后12、24、36和48 h （hpi）在肝胰腺、鳃、肌肉和肠道中均不同程度地显著上调表达。此外，透射电镜（TEM）在感染对虾的肝胰腺细胞中观察到许多自溶酶体。最终，我们确定了与罗氏对虾免疫应答相关的自噬相关基因，为阐明自噬在对虾先天免疫机制中的作用提供了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.