Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Transcriptomic signatures associated with developmental transitions in the gill of lake sturgeon (Acipenser fulvescens)","authors":"William S. Bugg ,&nbsp;Madison L. Earhart ,&nbsp;Matt J. Thorstensen ,&nbsp;Patricia M. Schulte ,&nbsp;W. Gary Anderson ,&nbsp;Ken M. Jeffries","doi":"10.1016/j.cbd.2025.101505","DOIUrl":"10.1016/j.cbd.2025.101505","url":null,"abstract":"<div><div>The developmental transition from the larval to juvenile stage, represents one of the highest periods of mortality for many species of fish. Thus, understanding the factors that may influence susceptibility to environmental stressors at this vulnerable stage is likely to be important for predicting responses to environmental change. Here, we compared gill mRNA transcript abundance during this critical developmental transition in fish from two geographically distinct populations of lake sturgeon (<em>Acipenser fulvescens</em>) that experience highly divergent thermal environments. Throughout the developmental transition from the larval to juvenile stage (30 to 60 days post-fertilization), the ‘colder’ northern population exhibited differential expression of approximately twice as many transcripts as compared to their ‘warmer’ southern counterparts. Transcriptional modifications that were conserved across both populations during the transition from the larva to juvenile stage were involved in histone methylation, protein binding, and apoptotic signaling associated with cell death and turnover. The northern population, specifically, had increased changes associated with GTPase signaling activation, cell surface proteins, mitochondrial and epithelial development which may have contributed to the larger magnitude of transcriptional change in this population, compared to their southern counterparts. Together these findings demonstrate the impacts of transcriptional shifts in the gill during development in lake sturgeon, as well as the potential for population-specific developmental processes.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101505"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4D-FastDIA proteomic analysis of pedal mucus in Pacific abalone Haliotis discus hannai heat-resistance group","authors":"Guilan Di ,&nbsp;Yu Zhang ,&nbsp;Mingmei Jiang ,&nbsp;Weini Zhang ,&nbsp;Yunlong Wu ,&nbsp;Zeyuan Ma ,&nbsp;Weilong Yang ,&nbsp;Yuqin Fu ,&nbsp;Xinhua Chen","doi":"10.1016/j.cbd.2025.101503","DOIUrl":"10.1016/j.cbd.2025.101503","url":null,"abstract":"<div><div>This study aimed to investigate the immune mechanisms of pedal mucus in Pacific abalone Haliotis discus hannai from different populations. Proteomic differences between the pedal mucus of the control group and the heat-resistance group of H. discus hannai were comparatively analyzed, and the proteins were annotated and analyzed to understand the related functions and roles of differentially expressed proteins (DEPs). The results showed that there were 4054 DEPs in total, among which the DEPs in the pedal mucus of the heat-resistance group and the control group were mainly immune-related heat shock proteins, calmodulin, Ig-like and fibronectin type III domain-containing proteins, histones and mucins, etc.; the DEPs related to growth metabolism included glutathione, growth factor receptor-bound protein, alanine aminotransferase, etc. Circadian entrainment signaling pathway and growth hormone synthesis, secretion and action signaling pathway was significantly enriched, cortisol synthesis and secretion signaling pathway was up-regulated in Haliotis discus hannai heat-resistance group. This study provided references for exploring the relationship between the mucus proteome difference in pedal mucus from different abalone populations sources. These results would contribute to further search for proteins related to immunity, growth and stress resistance, and provide theoretical basis for the development of high-quality germplasm resources of Pacific abalone.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101503"},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional atlas of Daphnia magna","authors":"Ishaan Dua ,&nbsp;Lev Y. Yampolsky","doi":"10.1016/j.cbd.2025.101504","DOIUrl":"10.1016/j.cbd.2025.101504","url":null,"abstract":"<div><div>Transcriptomics studies are more likely to achieve predictive results when they rely on tissue- and cell-specific transcriptional data. Identification of cell types in novel model organisms by their transcriptional profiles is difficult without data on transcriptional differences among major tissues and anatomical features. Here we report the first dataset on tissue- and organ-specific transcriptomics in freshwater plankton crustacean <em>Daphnia magna</em>, reporting markers of embryos, hemocytes, gut, carapace, antennae-2, and head, as well as the remaining carcass. Embryos are the most transcriptionally different from adults' features, with antennae and carapace being the most differentiated among them. We demonstrate that transcriptional markers of embryos vs. adults and of various adult anatomical features can be used to provide validation and functional explanation to published differential expression in response to environmental factors like infection, hypoxia, toxicants, or kairomones; to annotate <em>Daphnia</em> single cell data; and to ask questions about transcriptional diversification within extended gene families.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101504"},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the potential impact of sustained high temperatures during non-breeding season on largemouth bass","authors":"Yue Chen ,&nbsp;Jun Wang ,&nbsp;Chun Xiu Jin ,&nbsp;Hao Wu ,&nbsp;Wei He ,&nbsp;Zi Xian Wu ,&nbsp;Zi Tong Wang ,&nbsp;Yi Zhou Hong ,&nbsp;Zi Hang Yang ,&nbsp;Song Yang ,&nbsp;Fei Biao Song ,&nbsp;Jian Luo ,&nbsp;Jun Long Sun","doi":"10.1016/j.cbd.2025.101501","DOIUrl":"10.1016/j.cbd.2025.101501","url":null,"abstract":"<div><div>With the growing scale of largemouth bass breeding, the demand for seedlings is increasing. As global temperatures rise, it is crucial to study the effects of high temperature their regulatory mechanisms in largemouth bass. In this study, we simulated a high water temperature (28 °C) in the non-breeding season in aquaculture ponds for 28 days to examine the growth, reproduction, metabolism, apoptosis, and methylation markers in largemouth bass; transcriptome analysis was also performed. The results showed no significant difference in body weight between male and female largemouth bass. However, the high-temperature exposed females had reduced growth hormone (GH) and estradiol (E2) levels and elevated cortisol levels. They also showed upregulated expression of <em>AR</em>, <em>cyp19a</em>, <em>igf</em>, <em>fshβ</em>, and <em>lhβ</em> in ovarian tissue. Transcriptomic comparisons between temperature treatments revealed 963 differentially expressed genes in females and 700 in males. Both the ECM receptor interaction and PPAR signaling pathways were significantly enriched. High-temperature enhanced the lipid metabolism process through the PPAR signaling pathway. High temperatures increased oxidative stress in females, which corresponded with increases in SOD, CAT, and GSH-Px, likely to counteract the excess reactive oxygen species. Moreover, endoplasmic reticulum stress was activated, indicated by increases in IRE1 and ATF6, leading to the upregulation of apoptosis-related genes and ovarian cell apoptosis. At high temperature, 5-MC%, demethylase, and methyltransferase were not different in females, while 5-MC% and methyltransferase were higher and demethylase was lower in males. In summary, sustained high temperature affected ovarian development by altering the expression of hormone and gonad related genes and inducing endoplasmic reticulum stress leading to ovarian cell apoptosis. However, low demethylase activity and high genome-wide methylation in the test is suggested that high temperatures may affect testis development via methylation, potentially impacting offspring production.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101501"},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of sulfate on the physiology, biochemistry, and intestinal transcriptome of Scylla paramamosain under low-salinity conditions","authors":"Gao Gao ,&nbsp;Yun Hu ,&nbsp;Kangxiang Qin ,&nbsp;Ziwei Fan ,&nbsp;Chunlin Wang ,&nbsp;Huan Wang","doi":"10.1016/j.cbd.2025.101502","DOIUrl":"10.1016/j.cbd.2025.101502","url":null,"abstract":"<div><div>Sulfate, a key component of seawater and sulfate-saline-alkali soil, critically impacts aquatic species' physiology. The aim of this experiment was to investigate the effects of sulfate concentration on the growth, survival, microstructure of gills and hepatopancreas, as well as the intestinal immune response and molecular mechanisms of <em>Scylla paramamosain</em>. The experimental groups were categorized as S1 (0 g/L sulfate, sulfate-free), S2 (1 g/L sulfate, optimal concentration), and S3 (2 g/L sulfate, high sulfate concentration). The results showed that with the increase of sulfate concentration in the experimental water, Survival and weight growth rates initially increased but declined at higher sulfate concentrations (<em>P</em> &lt; 0.05), while molting rates progressively decreased. The intestinal ACP and AKP levels in S2 were significantly higher than those in S1 and S3 (<em>P</em> &lt; 0.05). AST and ALT in the S2 group showed no significant difference from those in the S3 group (<em>P</em> &gt; 0.05) but were significantly lower than those in the S1 group (<em>P</em> &lt; 0.05). Sulfotransferases in the S2 group were significantly lower than those in the S1 and S3 groups (<em>P</em> &lt; 0.05). Short-term (72 h) microscopic observations of the gills and hepatopancreas revealed that the gills in both the S1 and S3 groups exhibited thickening of the gill filaments and rupture of the cuticle layer, while the hepatopancreatic tissue in the S3 group also showed enlargement of intercellular spaces, fracture of the basement membrane, and blurred boundaries. Transcriptome sequencing of the <em>S. paramamosain</em> intestine revealed significant upregulation of sulfotransferases in both S1 and S3 groups. GO annotation of DEGs indicated similar responses of <em>S. paramamosain</em> to sulfate-free and high-sulfate environments, maintaining physiological stability by activating protein processing pathways, enhancing blood-lymph coagulation, and regulating redox balance. KEGG analysis revealed that sulfate-free environments predominantly enriched lipid metabolism and secondary metabolism pathways. While those enriched in high-sulfate environments were mainly related to amino acid metabolism and secondary metabolism. The results indicate that both sulfate-free and high-sulfate concentrations affect the growth, survival, and molting rates of <em>S. paramamosain</em>, as well as inducing immune responses in the intestine. In the short term, they can cause structural changes in the gills and hepatopancreas. The intestinal transcriptome expression shows a similar response pattern, but the metabolic pathways differ. This study provides theoretical support and technical guidance for the cultivation of <em>S. paramamosain</em> under low-salinity conditions with sulfate-type saline-alkali water.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101502"},"PeriodicalIF":2.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The physiological and transcriptional differences between golden and brown noble scallops Chlamys nobilis","authors":"Cheng He,&nbsp;Yizhou Sun,&nbsp;Xixi Duan,&nbsp;Weili Wang,&nbsp;Chuanxu Zhang,&nbsp;Hongkuan Zhang,&nbsp;Huaiping Zheng","doi":"10.1016/j.cbd.2025.101498","DOIUrl":"10.1016/j.cbd.2025.101498","url":null,"abstract":"<div><div>Mollusks are the second Phylum in the Kingdom Animalia, which provide not only high-quality protein but also serves as a viewing function for humans due to the polymorphism in shell colors. However, the mechanisms of the differences in shell colors, especially on scallops, have rarely been studied. In this study, the shell and mantle of two different shell colors (golden and brown) of the noble scallops <em>Chlamys nobilis</em> were analyzed, including their microstructure, pigment content, and gene expression. The results showed that the golden scallop shell is golden from the outside to the inside, while the prismatic and nacreous layers in the brown scallop are lighter in color than the periostracum. Unlike the golden scallop, there was a layer of melanin vesicles at the edge of the brown scallop mantle. The total carotenoids content (TCC) in the mantle and shell of the golden scallop was significantly higher than that of the brown scallops (<em>P</em> &lt; 0.05), but the melanin content was significantly lower than that of the brown scallop (<em>P</em> &lt; 0.05). Candidate genes such as <em>BCDO1</em>, <em>CYP5A</em>, <em>CYP2J</em>, <em>CYBA</em>, <em>EP300</em>, and <em>GNAO</em> were screened from the differentially expressed genes (DEGs), and their differential expression may explain the differences in melanin and carotenoid content between the golden and brown scallops. These findings will help to understand the color polymorphism of noble scallops and provide a basis for further research on the inheritance of noble scallops.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101498"},"PeriodicalIF":2.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and expression profiling of the hemoglobin gene family under hypoxia stress in Sillago sihama","authors":"Lingwei Kong ,&nbsp;Minghui Ye ,&nbsp;Jiru Tan ,&nbsp;Wenjing Lai ,&nbsp;Jiaxin Liao ,&nbsp;Yulei Zhang ,&nbsp;Huapu Chen ,&nbsp;Yang Huang ,&nbsp;Guangli Li ,&nbsp;Changxu Tian","doi":"10.1016/j.cbd.2025.101500","DOIUrl":"10.1016/j.cbd.2025.101500","url":null,"abstract":"<div><div>Hemoglobin (Hb) plays a pivotal role in oxygen transport and is essential for the adaptive response to hypoxic stress. Studying the molecular evolution characteristics and expression pattern of <em>S. sihama</em> Hb gene family can provide theoretical basis for further revealing the roles of the <em>Hb</em> genes against hypoxia stress. This study aimed to identify the molecular evolution characteristics and expression patterns of <em>Hb</em> genes in <em>S. sihama</em>. Utilizing bioinformatics methods, we identified ten members of the <em>Hb</em> gene family within the whole genome of <em>S. sihama</em>, located on chromosomes LG16 and LG21 in a tandem repeat pattern. Phylogenetic analysis classified the Hb gene family into two subfamilies (alpha and beta), showing close relationships with the Hb gene family members of the large yellow croaker (<em>Larimichthys crocea</em>). Both phylogenetic and collinearity analysis indicated the conservation of <em>Hb</em> genes throughout evolution. The nonsynonymous substitution rate / synonymous substitution rate (<em>Ka</em>/<em>Ks</em>) results showed that its evolution was purifying selection. RNA-seq and qRT-PCR analyses revealed that five <em>Hb</em> genes were highlighted high expression levels in the heart and liver of <em>S. sihama</em>, with <em>sshba1.4</em>, <em>sshbad</em>, and <em>sshba1</em> showing significant up-regulation in response to hypoxic stress. In summary, this study highlights the vital role of the Hb gene family in the hypoxic stress response of <em>S. sihama</em>, suggesting their potential as crucial candidate genes for regulating environmental stress in this species.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101500"},"PeriodicalIF":2.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and proteomic analyses reveal the diverse components in the venom of a recently described spider species Macrothele washanensis","authors":"Mengmeng Zhang ,&nbsp;Wenzheng Cai ,&nbsp;Menghui Yang ,&nbsp;Mengying Zhang ,&nbsp;Luke R. Tembrock ,&nbsp;Zhibin Yang ,&nbsp;Heng Liu ,&nbsp;Zizhong Yang","doi":"10.1016/j.cbd.2025.101491","DOIUrl":"10.1016/j.cbd.2025.101491","url":null,"abstract":"<div><div>Spider venom has been shown to possess a variety of pharmacological activities with promising applications in biomedical and agricultural sciences. However, the molecular structure and functions of these toxins have only been revealed for a few species. In this study, we unveiled the venom composition of <em>Macrothele washanensis</em> venom for the first time using transcriptomics, proteomics, and bioinformatic analyses. A total of 147,492,716 Illumina valid reads were obtained from the transcriptome of the venom glands, and 3,010,024 unigenes were assembled to predict 69 putative toxin sequences, which were classified into 25 toxin superfamilies based on cysteine motifs. Crude venom proteomics analysis identified 43 toxin proteins, including 15 peptide toxins and 28 proteases, including the CAP (CRISP/Allergen/PR) protein family, acetylcholinesterase, metalloproteinases, hyaluronidase, and dermonecrotic toxin. In conclusion, the results of the present study revealed the composition of the venom of <em>M. washanensis</em>, and several peptide toxins with potential medical, such work provides the foundation for further research on the molecular functions of these venom components as well as applied studies for the use of such compounds.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101491"},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chronic high-temperature stress on muscle tissue integrity and metabolism-related genes in Clarias fuscus","authors":"Xiaolong Li ,&nbsp;Yong Liu ,&nbsp;Cunyu Duan ,&nbsp;Lei Yang ,&nbsp;Dayan Zhou ,&nbsp;Zhixin Zhang ,&nbsp;Huapu Chen ,&nbsp;Guangli Li ,&nbsp;Chunhua Zhu ,&nbsp;Changxu Tian","doi":"10.1016/j.cbd.2025.101497","DOIUrl":"10.1016/j.cbd.2025.101497","url":null,"abstract":"<div><div>The increasing prevalence of high-temperature days due to global warming presents significant challenges for aquatic ecosystems and aquaculture practices. This study investigates the effects of chronic high-temperature stress on <em>Clarias fuscus</em>, a catfish species native to subtropical and tropical regions. The fish were cultured for 90 days under high-temperature conditions (HT, 34 °C) and normal temperature conditions (CT, 26 °C). Histological and transcriptomic analyses were conducted to assess the impact of continuous high-temperature stress on muscle tissue. Histological examination revealed significant damage in the HT group, characterized by irregular tissue arrangement, widened muscle fiber gaps, broken muscle filaments, and cracked nuclei. Transcriptomic analysis identified 975 differentially expressed genes (DEGs) in muscle tissue under high-temperature stress, with 512 genes up-regulated and 463 down-regulated. Notably, heat shock protein (Hsp) family genes, including <em>Hsp40</em>, <em>Hsp70</em> and <em>Hsp90</em>, were significantly up-regulated under heat stress. Enrichment analysis of these DEGs revealed significant alterations in protein processing, the PPAR signaling pathway, and fatty acid oxidation and metabolism within the endoplasmic reticulum. These findings suggest that <em>C. fuscus</em> experiences substantial tissue damage and a reduced metabolic response under high-temperature stress. This study provides a scientific foundation for future research on the adaptability of fish to temperature fluctuations.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101497"},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles","authors":"Silvina A. Belliard ,&nbsp;Juan Hurtado ,&nbsp;Luciano M. Matzkin ,&nbsp;Santiago Revale ,&nbsp;Diego F. Segura","doi":"10.1016/j.cbd.2025.101495","DOIUrl":"10.1016/j.cbd.2025.101495","url":null,"abstract":"<div><div><em>Anastrepha fraterculus</em> is a major pest of fruits in South America. Previous studies revealed that males exposed to volatiles from guava, a likely ancestral host, intensify courtship behavior and increase mating success. However, the molecular and physiological mechanisms underlying this effect remain unknown. Here, we explore the impact of exposure to guava essential oil volatiles on the gene expression profile of adult male heads through de novo transcriptome assembly and differential expression analysis. We found 678 differentially expressed genes (FDR &lt; 0.05 and |FC| &gt; 2), 308 of which were over-expressed in exposed males and 370 in non-exposed males. The exposure treatment affected the transcription of genes putatively involved in mucus-forming, lipid metabolism and neuropeptide processing. Our findings provide the first insights into the molecular mechanisms underlying sexual stimulation in <em>A. fraterculus</em> males triggered by host fruit volatiles.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101495"},"PeriodicalIF":2.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}