Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Selection of heat-resistant strains in Bohai Red Scallops and their transcriptomic responses to heat stress","authors":"Qixiang Bo ,&nbsp;Peican Zhu ,&nbsp;Lisen Wen ,&nbsp;Peizhen Tian ,&nbsp;Haochen Wang ,&nbsp;Junhao Ning ,&nbsp;Guilong Liu ,&nbsp;Weidong Li ,&nbsp;Haien Zhang ,&nbsp;Chunde Wang ,&nbsp;Bo Liu","doi":"10.1016/j.cbd.2025.101541","DOIUrl":"10.1016/j.cbd.2025.101541","url":null,"abstract":"<div><div>Temperature has consistently been a critical environmental factor in the cultivation of scallops, and with global warming, marine animals will likely experience even more exposure to heat stress. Thus, it is necessary to select heat-resistant strains in scallops. In this study, heat stress selection was performed on the unselected Bohai Red scallop (CG) using the measured upper median lethal temperature (LT₅₀), yielding an elite group (EG), from which the heat-resistant F1 offspring (EGF1) were reproduced. Tolerance to heat stress was significantly increased in EGF1, as evidenced by the elevated LT₅₀ and the activities of certain antioxidant enzymes. Transcriptomic analyses and GO and KEGG analyses demonstrated that both EG and CG animals may mitigate heat stress and maintain cellular homeostasis through pathways associated with protein folding, energy metabolism, and antioxidant mechanisms. Furthermore, the scallops in the EG group showed a strong immune profile, with immune-related DEGs enriched mainly in interleukin-1 receptor binding and Toll-like receptor pathways. Conduct GO and KEGG enrichment analyses on the MM.darkolivegreen module, which demonstrates the strongest positive correlation with heat stress in Weighted Gene Co-expression Network Analysis (WGCNA). These analyses revealed pathways consistent with those identified in differentially expressed genes (DEGs), thereby providing mutual validation of the results. These results enhance our understanding of the molecular mechanisms driving heat stress adaptation in Bohai Red scallops and establish a theoretical foundation for the inheritance and evolution of heat-resistant traits.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101541"},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of candidate leg chemosensory genes associated with nursing behavior in the Apis mellifera","authors":"Jiaoxin Xie ,&nbsp;Huiman Zhang ,&nbsp;Lele Sun ,&nbsp;Yu Zhang ,&nbsp;Yuan Guo","doi":"10.1016/j.cbd.2025.101540","DOIUrl":"10.1016/j.cbd.2025.101540","url":null,"abstract":"<div><div>It is commonly recognized that legs play a vital role in the chemoreception of insects. The chemosensilla distributed on the legs of the worker bee <em>Apis mellifera</em> are involved in olfactory and gustatory detection. The responses of worker bees of different ages to various compounds were detected using an electrolegogram test on the legs. It was found that a high concentration of the larval starvation pheromone ocimene could trigger electrophysiological responses in 10-day-old worker bees (nurses). Through RNA transcriptome sequencing, the chemosensory proteins in the forelegs, middle legs, and hind legs of nurse bees that may be involved in adult close or contact chemical communication were studied. A total of 56 candidate chemosensory-related proteins, including 17 odorant-binding proteins (OBPs), 5 chemosensory proteins (CSPs), 6 gustatory receptors (GRs), 3 ionotropic receptors (IRs), 2 sensory neuron membrane proteins (SNMPs), and 23 odorant receptors (ORs), were identified from the assembled leg transcriptome. In general, the genes coding for OBPs and CSPs had significant but highly fluctuating TPM values, indicating high-level expression in the legs of nurses. In contrast, the chemosensory receptors ORs, GRs, and IRs showed low-level expression. In particular, <em>AmelOBP21</em>, <em>AmelCSP3</em>, <em>AmelOR170</em>, <em>AmelGR3</em>, <em>AmelIR21</em>, and <em>AmelSNMP1</em>, which were relatively highly expressed, may play olfactory and gustatory functions in the process of nurse bees recognizing larval starvation signals and initiating nursing behaviors. These results lay a foundation for further research on the chemoreception of legs in the honey bee <em>A. mellifera</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"55 ","pages":"Article 101540"},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-specific transcriptome analysis in Propsilocerus akamusi provides novel insights into the regulatory mechanisms under deltamethrin exposure","authors":"Xiaoya Sun,&nbsp;Yuanyuan Peng,&nbsp;Junhao Zhu,&nbsp;Yahan Pan,&nbsp;Deyu Wang,&nbsp;Lingfei Meng,&nbsp;Wenbin Liu,&nbsp;Chuncai Yan","doi":"10.1016/j.cbd.2025.101542","DOIUrl":"10.1016/j.cbd.2025.101542","url":null,"abstract":"<div><div>Deltamethrin is extensively utilized in agricultural pest control and its toxicological impacts on aquatic insects have drawn significant attention. However, the mechanisms of deltamethrin modulates gene expression framework in different tissues remain largely unknown. Herein, we selected non-biting midge, <em>Propsilocerus akamusi</em> (Diptera: Chironomidae), to investigate the gene repertoires in hemolymph, fat body, midgut and Malpighian tubules under the different concentrations of deltamethrin. Distinct tissue-specific gene expression patterns and pH levels were found in the response of <em>P. akamusi</em> to deltamethrin. Deltamethrin induced transcriptional perturbations in cuticle formation-, xenobiotic detoxification-, oxygen transport, and calcium signaling-related genes. The midgut and fat body mainly functioned on oxygen transportation, with the Malpighian tubules engaging in the calcium-ion transmembrane transport, and the overexpression of cuticular protein genes found in the hemolymph. In addition to the key role of detoxification genes, we inferred that deltamethrin perhaps activates calcium signaling pathways that protect against toxins found in the hemolymph and Malpighian tubules. Both organs exhibited upregulation of calcium/calmodulin-dependent protein Kinase II (<em>CaMKII</em>) and voltage-gated calcium channel (<em>VGCC</em>) genes, along with a rise in the pH level. Furthermore, we have compared the tertiary structures of the <em>CaMKII</em> genes in <em>P. akamusi</em> and identified <em>CaMKII</em> members in six other chironomids to explore the characteristics of these species that inhabit adverse environments. Our findings revealed the tissue-specific gene framework for <em>P. akamsui</em>, with particularly high expression detected in the hemolymph, which is instrumental in seeking target organs to develop effective biomarkers for water quality measurement.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101542"},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional landscape of brain adaptation to artificial feed domestication in mandarin fish (Siniperca chuatsi) revealed by weighted gene co-expression network analysis","authors":"Dianyang Zhou ,&nbsp;Jianming Chen ,&nbsp;Zhennan Sun ,&nbsp;Haiqi Zhang ,&nbsp;Mingzhe Yuan ,&nbsp;Xubo Wang","doi":"10.1016/j.cbd.2025.101543","DOIUrl":"10.1016/j.cbd.2025.101543","url":null,"abstract":"<div><div>Mandarin fish (<em>Siniperca chuatsi</em>) initially reject dead prey or artificial feed, yet their innate feeding behaviors can be modified through training. However, the changes in gene expression in the brain and the associated molecular mechanisms underlying the acquisition of new feeding behaviors in mandarin fish are not well understood. In this study, two groups of mandarin fish were established: one group that was easily domesticated and transitioned from live prey to artificial diets (QN group), and another group that failed to adapt to artificial diets throughout the domestication process (DN group). The results showed that the QN group had significantly greater body weight and length compared to the DN group. Transcriptome analysis identified 811 differentially expressed genes (DEGs), with 393 genes upregulated and 418 downregulated. Functional enrichment analysis revealed significant enrichment in the steroid biosynthesis pathway. Weighted gene co-expression network analysis (WGCNA) identified brown and turquoise modules strongly correlated with high artificial feed intake, containing 1849 genes. KEGG analysis further indicated that the steroid biosynthesis pathway, especially progesterone synthesis, plays a key role in the dietary adaptation process. RT-qPCR and ELISA validation of transcriptome data confirmed the upregulation of progesterone levels, closely associated with food habit domestication. It was therefore speculated that the mandarin fish's ability to consume artificial diets might be attributed to the upregulation of genes associated with steroid biosynthesis, which in turn promotes progestogen synthesis. This study suggested a link between steroid biosynthesis and dietary domestication in animals, potentially revealing a novel molecular mechanism underlying dietary domestication.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101543"},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liver and intestine transcriptome analysis reveals molecular mechanisms of phytase-driven nutrient utilization and metabolic regulation in hybrid catfish","authors":"Dongdong Zhang ,&nbsp;Eric Peatman ,&nbsp;Benjamin H. Beck ,&nbsp;Honggang Zhao ,&nbsp;James Mazzola ,&nbsp;Baofeng Su ,&nbsp;Ahmed Elaswad ,&nbsp;Zhi Ye","doi":"10.1016/j.cbd.2025.101539","DOIUrl":"10.1016/j.cbd.2025.101539","url":null,"abstract":"<div><div>The use of phytase in aquafeeds has gained increasing attention as a strategy to enhance nutritional value and mitigate the adverse effects of phytic acid, especially for diets containing plant-based ingredients. Notwithstanding examples of phytase-induced phenotypic changes, the molecular mechanisms underlying phytase supplementation are not well understood. The present study evaluated the effects of phytase on the transcriptomic profiles in the liver and intestine, as well as on growth, feed conversion ratio (FCR), and hematological parameters of Jubilee × D&amp;B hybrid catfish. Over a 140-day feeding trial, phytase supplementation (2500 phytase units/kg diet) significantly improved growth, FCR, red blood cell count, hematocrit, and total cell count in the blood compared with fish fed the basal diet. By comparing the transcriptomic profiles of phytase-supplemented and control fish, we identified a distinct gene expression profile relative to controls. This profile was characterized by differentially expressed genes (DEGs) associated with mineral metabolism (including iron), energy homeostasis, protein synthesis, carbohydrate and lipid metabolism, and immune response. The putative roles of key DEGs, including their interactions in different metabolic pathways, are discussed. The current study explains the benefits of phytase supplementation on hybrid catfish performance on the molecular level, uncovers the transcriptomic mechanisms controlling these benefits, and provides valuable information for customized functional feeds in aquaculture.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101539"},"PeriodicalIF":2.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative miRNA transcriptome analysis reveals miR-375-3p targets cyp19a and regulates ovarian development in Medaka (Oryzias latipes)","authors":"Xinlan Ma ,&nbsp;Haijing Xu ,&nbsp;Tengyang Lu,&nbsp;Wenkai Lin,&nbsp;Lulu Sun,&nbsp;Muhammad Jawad,&nbsp;Mingyou Li","doi":"10.1016/j.cbd.2025.101535","DOIUrl":"10.1016/j.cbd.2025.101535","url":null,"abstract":"<div><div>In teleosts, hormonal secretion from the pituitary gland exhibits significant sex plasticity, potentially linked to gonadal differentiation through the hypothalamic-pituitary-gonadal (HPG) axis. miRNAs, as transcriptional regulators, affecting gene expression by promoting mRNA degradation or suppressing translation. To explore the association between miRNAs and <em>cyp19a</em> and their potential role in reproduction, RNA sequencing (RNA-Seq) was conducted on male and female medaka brains. A total of 54 miRNAs were identified as significantly differentially expressed, with 45 showing female-biased expression and 9 exhibiting male-biased expression. qPCR analysis revealed that the expression levels of candidate miRNAs significantly differed between males and females as sex differentiation progressed. Additionally, <em>cyp19a1a</em> was highly expressed in the ovary, while <em>cyp19a1b</em> showed high expression in the brain. Treatment with the aromatase inhibitor exemestane (EM) induced male characteristics, leading to noticeable ovarian degeneration and cavitation. Following EM treatment, qPCR analysis showed an increase in miRNA expression, alongside a decrease in female development-related genes (<em>foxl2</em>, <em>cyp19a1a</em>, and <em>cyp19a1b</em>) and an increase in male development-related genes (<em>dmy</em>, <em>cyp17a</em>, and <em>gsdf</em>), compared to the control group. Additionally, a dual-luciferase reporter assay confirmed that miR-375-3p specifically binds to <em>cyp19a1a</em> and <em>cyp19a1b</em>. In conclusion, this study enhances the understanding of miRNA regulatory mechanisms in the brain, gonads, and reproductive development of medaka.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101535"},"PeriodicalIF":2.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-omics analysis and RNAi reveal Toll signaling pathway and melanization as key defenses in Ostrinia furnacalis against Meteorus pulchricornis parasitism","authors":"Ziyuan Tang ,&nbsp;Gui Gao ,&nbsp;Zhilin Xia ,&nbsp;Changli Yang ,&nbsp;Changyu Zhang","doi":"10.1016/j.cbd.2025.101537","DOIUrl":"10.1016/j.cbd.2025.101537","url":null,"abstract":"<div><div><em>Ostrinia furnacalis</em>, a worldwide agricultural pest, can be effectively controlled using the endoparasitic wasp <em>Meteorus pulchricornis</em>. However, the molecular mechanisms underlying the host immune response to parasitism remain poorly understood. This study aimed to explore the possible immune molecular mechanisms of <em>O. furnacalis</em> under parasitism by <em>M. pulchricornis</em> for 24 and 48 h (P24 and P48). Overall, 97 differentially expressed genes (DEGs) and 18 differentially expressed proteins (DEPs) associated with immunity were identified, including pattern-recognition receptors (PRRs), antimicrobial peptides (AMPs), antioxidant enzymes, Toll signaling pathway and melanization components. Three Toll signaling pathway downstream genes <em>OfMyD88</em>, <em>OfGlov</em>, and <em>OfLys</em> were chosen for functional analysis by RNA interference (RNAi). The results showed a notable increase in parasitism rate (7–9 %) in immunocompromised hosts and a significant decrease in survival rates (23–27 %), demonstrating that <em>OfMyD88</em>, <em>OfGlov</em>, and <em>OfLys</em> play crucial roles in the parasitic stress response and larvae survival. Our findings reveal novel insights into the immune molecular mechanisms of <em>O. furnacalis</em> and highlight potential targets for enhancing parasitic wasp-based biocontrol strategies.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101537"},"PeriodicalIF":2.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics analysis provides new insights into the ovarian lipid droplet formation and lipid deposition in Plectropomus leopardus","authors":"Changqing He ,&nbsp;Youheng Huang ,&nbsp;Silvana Rahayu ,&nbsp;Hao Liu ,&nbsp;Yang Huang ,&nbsp;Gang Shi ,&nbsp;Huapu Chen ,&nbsp;Guangli Li ,&nbsp;Chunhua Zhu ,&nbsp;Mouyan Jiang","doi":"10.1016/j.cbd.2025.101534","DOIUrl":"10.1016/j.cbd.2025.101534","url":null,"abstract":"<div><div>The leopard coral grouper (<em>Plectropomus leopardus</em>), an increasingly important species in marine aquaculture, has garnered significant research interest due to its high market value. Despite extensive research on ovarian growth and development in fish, the molecular mechanisms governing lipid droplet formation and lipid deposition in <em>P. leopardus</em> remain poorly understood. In this study, we conducted transcriptomic analyses of <em>P. leopardus</em> ovaries at three developmental stages: primary growth (PG), pre-vitellogenesis (PV), and mid-vitellogenesis (MV). A total of 534,847,090 raw reads were obtained from nine cDNA libraries, leading to the identification of 19,155 genes with 13,817 genes expressed at all stages. Differential analysis showed that 1012, 2609, and 4039 genes were up-regulated, while 168, 277, and 577 genes were down-regulated in the three comparisons, respectively. Functional enrichment analyses highlighting the critical roles of differentially expressed genes (DEGs) in lipid transport (such as <em>fatp1</em>, <em>fatp4</em>, <em>fatp6</em>, <em>apoeb</em>, <em>lpl</em> and <em>fabps</em>), fatty acid metabolism (such as <em>elovl6, acsl1</em>, <em>dgat2</em> and <em>gpat4</em>) and phospholipid metabolism (such as <em>ept1</em>, <em>chka</em> and <em>pla2g15</em>). These findings underscore their contribution to lipid droplet formation and deposition. Furthermore, key signaling pathways, including Wnt, mTOR, PPAR and PI3K/Akt, were implicated in regulating these processes. The reliability of the RNA-seq data was confirmed through qPCR validation of 10 lipid-related genes. Based on these results, we propose a model for lipid droplet formation and lipid deposition during ovarian development in <em>P. leopardus</em>. This study advances our understanding of ovarian development in <em>P. leopardus</em> and provides a foundation for future research on marine fish reproduction, with potential applications in species conservation and aquaculture management.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101534"},"PeriodicalIF":2.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-organ transcriptomics provide insights into growth regulation in the Dongtingking crucian carp (Carassius auratus indigentiaus)","authors":"Dan Zeng ,&nbsp;Yunsheng Zhang ,&nbsp;Hu Xia ,&nbsp;Liangguo Liu ,&nbsp;Yangyang Tu ,&nbsp;Mengying Chen ,&nbsp;Pinghong Yang","doi":"10.1016/j.cbd.2025.101538","DOIUrl":"10.1016/j.cbd.2025.101538","url":null,"abstract":"<div><div>To investigate the key regulatory genes and pathways related to growth traits in the Dongtingking crucian carp (<em>Carassius auratus indigentiaus</em>), the transcriptomes of brain, intestine, and muscle tissues were sequenced at early juvenile stage using RNA-Seq from two groups with extreme growth rates (fast-growing and slow-growing). A total of 65, 184, and 130 differentially expressed genes (DEGs) were detected in the brain, intestine, and muscle, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis highlighted that the PPAR signaling pathway, Insulin/PI3K/Akt/mTOR/FoxO/AMPK pathway, and Protein digestion and absorption pathways are crucial for growth in this species. Based on the transcriptome data, 32 key DEGs were identified, mainly participating in processes such as cell proliferation and differentiation, growth, development, and metabolism. Prominent examples are cyclic AMP-responsive element-binding protein 5 (<em>creb5b</em>), forkhead box protein O1-A (<em>foxo1a</em>), transcription factor AP-1-like (<em>jun</em>), lipoprotein lipase-like (<em>lpl</em>), angiopoietin-like 4 (<em>angptl4</em>), and egl nine homolog 3-like (<em>egln3</em>). This study enhances the understanding of the genetic factors and regulatory mechanisms responsible for variations in growth rates and provides a valuable basis for further studies on the regulatory mechanisms of growth in <em>C. auratus indigentiaus</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101538"},"PeriodicalIF":2.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Papillae growth and molecular responses in sea cucumber (Apostichopus japonicus) exposed to light spectra","authors":"Ziyu Liu ,&nbsp;Shuchao Ruan ,&nbsp;Junhui Wang ,&nbsp;Weiyan Li ,&nbsp;Ye Tian ,&nbsp;Chong Zhao ,&nbsp;Lingshu Han ,&nbsp;Luo Wang ,&nbsp;Yaqing Chang ,&nbsp;Jun Ding","doi":"10.1016/j.cbd.2025.101536","DOIUrl":"10.1016/j.cbd.2025.101536","url":null,"abstract":"<div><div>This study primarily investigated the light spectra that increased the number of papillae in sea cucumbers and the molecular pathways that contributed to the growth of papillae under these light conditions. At 8.0—15.0 °C, sea cucumbers were subjected to a 60-day indoor experiment under different lighting environments (normal, dark, blue light, yellow light, green light, red light, and white light). The number of papillae was analyzed using repeated measures ANOVA, and transcriptome sequencing data of the papillae were subsequently analyzed. The analysis revealed that there were significant differences in the number of papillae within each group at the beginning of the experiment and on days 15, 30, and 45 of light exposure (<em>p</em> &lt; 0.05), while no significant differences were observed within each group between days 45 and 60 of light exposure (<em>p</em> &gt; 0.05). Under blue light exposure, the number of papillae and the increase in the number of papillae in sea cucumbers showed significant differences compared to the control group (<em>p</em> &lt; 0.05). The pathways related to the increase in the number of papillae in sea cucumbers include phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolism. The number of papillae increased the most after 45 days of blue light exposure, after which it stabilized. It was speculated that the increase in the number of papillae might be related to amino acid metabolism and collagen synthesis.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101536"},"PeriodicalIF":2.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}