Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Evolutionary origin and distribution of leucine-rich repeat-containing G protein-coupled receptors in insects","authors":"Ying Tian,&nbsp;Han Gao,&nbsp;Hong Li,&nbsp;Chengjun Li,&nbsp;Bin Li","doi":"10.1016/j.cbd.2024.101318","DOIUrl":"10.1016/j.cbd.2024.101318","url":null,"abstract":"<div><p>Leucine-rich repeat-containing G protein-coupled receptors (LGRs) are crucial for animal growth and development. They were categorized into four types (A, B, C1, and C2) based on their sequence and domain structures. Despite the widespread distribution of LGRs across bilaterians, a thorough investigation of their distribution and evolutionary history remains elusive. Recent studies insect LGRs, especially the emergence of type C2 LGRs in various hemimetabolous insects, had prompted our study to address these problems. Initially, we traced the origins of LGRs by exploiting data from 99 species spanning 11 metazoan phyla, and discovered that type A and B LGRs originated from sponges, while type C LGRs originated from cnidarians. Subsequently, through comprehensive genomic and transcriptomic analyses across 565 species across 25 orders of insects, we found that both type A and C1 LGRs divided into two gene clusters. These clusters can be traced back to basal Insecta and an early ancestor of the Arthropoda, respectively. Furthermore, the absence of type B LGRs in wingless insects suggests a role in wing development, while the absence of type C2 LGRs in holometabolous insects hints at novel functions unrelated to insect metamorphosis. According to the origin of LGRs and the investigation of LGRs in insects, we speculated that type A and B LGRs appeared first among four types of LGRs, type A evolved into type C LGRs later, and type A and C1 LGRs independently duplicated during the evolutionary process. This study provides a more comprehensive view of the evolution of LGR genes than previously available, and sheds light on the evolutionary history and significance of LGRs in insect biology.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome, histology, and enzyme activities analysis of liver in Phoxinus lagowskii to the low temperature stress and recovery","authors":"Mingyang Sun ,&nbsp;Cunrun Ye ,&nbsp;Zhen Wang ,&nbsp;Xinran Gao ,&nbsp;Shibo Feng ,&nbsp;Tingting Hu ,&nbsp;Weijie Mu","doi":"10.1016/j.cbd.2024.101317","DOIUrl":"10.1016/j.cbd.2024.101317","url":null,"abstract":"<div><p>Assessing the response and resilience of fish to low temperatures over different time scales can provide valuable insights into their mechanisms of adaptation to cold conditions. Farmed Amur minnows (<em>Phoxinus lagowskii</em>) frequently encounter low temperatures, especially during winter. However, the specific responses of <em>P. lagowskii</em> to low-temperature stress remain largely unexplored. In this study, we examined serum glucose and cortisol levels, histological changes, enzymes associated with phosphate and carbohydrate metabolism, triglyceride levels, and liver transcriptomics under various conditions: control (CK), short-term cold exposure (6 days, SC), prolonged cold exposure (14 days, PC), and recovery (RY) from cold exposure at 2 °C. Liver vacuolation was observed during short-term cold exposure. Additionally, we analyzed the enzymatic activity related to carbohydrate and lipid metabolism in serum and liver. Liver transcriptomic data revealed that the PPAR signaling pathway and autophagy-related genes were enriched during short-term cold exposure. Carbohydrate metabolism-related pathways, including the AMPK and MAPK signaling pathways, were significantly enriched after prolonged cold exposure. Metabolic pathways such as fat digestion and absorption, glycine, serine, and threonine metabolism, and arginine and proline metabolism were significantly enriched in the recovery group. Rapid warming after prolonged cold stress allowed <em>P. lagowskii</em> to recover quickly. These findings suggest that <em>P. lagowskii</em> has a strong adaptive capacity for energy metabolism during prolonged cold exposure and the ability to recover rapidly from cold stress. A comprehensive examination of the histological, physiological, biochemical, and molecular responses of <em>P. lagowskii</em> to low temperatures is crucial for developing effective strategies for cultivating this species in challenging environments.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142147170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic analysis revealed gender-related differences in the skin mucus proteome of discus fish (Symphysodon haraldi) during the parental and non-parental care periods","authors":"Shi-Chen Zhao ,&nbsp;Bin Wen ,&nbsp;Jian-Zhong Gao ,&nbsp;Zai-Zhong Chen","doi":"10.1016/j.cbd.2024.101314","DOIUrl":"10.1016/j.cbd.2024.101314","url":null,"abstract":"<div><p>The discus fish, <em>Symphysodon</em> spp., a South American cichlid, has a unique parental care behavior where fry bite on parental skin mucus after hatching. In this study, we used LC-MS/MS technique to compare the skin mucus proteome composition of male or female discus fish during parental and non-parental care periods. By multivariate statistical analysis, we found clear separations between different periods and between different sexes of mucus proteome. Compared with non-parental female fish, parental female fish had 283 up-regulated and 235 down-regulated expressed proteins. Compared with non-parental male fish, parental male fish had 169 up-regulated and 120 down-regulated expressed proteins. The differentially expressed proteins for male fish were enriched in sulfur relay system, mucin type O-glycan biosynthesis and antigen processing and presentation pathways, while those for female fish were enriched in sulfur relay system, steroid biosynthesis and complement and coagulation cascades pathways. During the parental care, both male and female discus showed an enhanced lipid metabolism, producing more phospholipids and cholesterol. The difference is that male discus had increased tricarboxylic acid cycle producing more energy during the parental care, while females produced more nucleotides especially guanylic acid. Our study could provide new insights into the understanding of the unique mucus supply behavior of discus fish based on proteomic change.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142087982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome mining and expression analysis of ABC transporter genes in a monophagous herbivore, Leucinodes orbonalis (Crambidae: Lepidoptera)","authors":"Santoshkumar Singamshetty ,&nbsp;Upasna Selvapandian ,&nbsp;Selva Babu Selvamani ,&nbsp;Suman Talya Chandrashekara ,&nbsp;Jyoti Pathak ,&nbsp;Aditi Agarwal ,&nbsp;Venkatesan Thiruvengadam ,&nbsp;Gandhi Gracy Ramasamy ,&nbsp;Satya Nand Sushil ,&nbsp;Muralimohan Kamanur ,&nbsp;Nagesha Nara ,&nbsp;Muthugounder Mohan","doi":"10.1016/j.cbd.2024.101316","DOIUrl":"10.1016/j.cbd.2024.101316","url":null,"abstract":"<div><p>Insecticide resistance is a global concern and requires immediate attention to manage dreadful insect pests. One of the resistance mechanisms adopted by insects is through ATP-binding cassette (ABC) transporter proteins. These proteins rapidly transport and eliminate the insecticidal molecules across the lipid membranes (Phase III detoxification mechanism). In the present study, we investigated the potential role of ABC transporter genes in imparting insecticide resistance in field-collected insecticide resistant larvae of eggplant shoot and fruit borer (<em>Leucinodes orbonalis</em>; Crambidae: Lepidoptera). Dose-mortality bioassays against five insecticidal molecules revealed moderate to high levels of insecticide resistance (32.2. to 134.1-fold). Thirty-one genes encoding ABC transporter proteins were mined from the transcriptome resources of <em>L. orbonalis</em>. They were classified under eight sub-families (<em>ABCA</em> to <em>ABCH</em>). Phylogenetic analysis indicated <em>ABCG</em> is the most divergent, composed of nine genes as compared to many other insects. Transcriptome analysis of the insecticide resistant and susceptible strains of <em>L. orbonalis</em> revealed differential expression of 13 ABC transporter genes. The altered expression of these genes was further validated using qRT-PCR. The knockdown studies indicated the involvement of <em>ABCD1</em> and <em>ABCG2</em> genes in chlorantraniliprole resistance in the insecticide-resistant strain of <em>L. orbonalis</em>. This study unveils the additional mechanisms employed by <em>L. orbonalis</em> in resisting insecticide toxicity through accelerated excretion mode. These <em>ABCD</em> and <em>ABCG</em> family genes could be candidate targets in developing genome-assisted pest management strategies in the future.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis provides insights into the immune responsive genes in the Procambarus clarkii hepatopancreas challenged with Vibrio parahaemolyticus","authors":"Jun-Jie Jiang ,&nbsp;Dan-Dan Bian ,&nbsp;Xin Liu ,&nbsp;Dai-Zhen Zhang ,&nbsp;Qiu-Ning Liu ,&nbsp;Bo-Ping Tang ,&nbsp;Mei-Ling Zhang","doi":"10.1016/j.cbd.2024.101315","DOIUrl":"10.1016/j.cbd.2024.101315","url":null,"abstract":"<div><p><em>Procambarus clarkii</em> is an economically important species in China; however, its high mortality rate due to pathogenic bacteria, particularly <em>Vibrio parahaemolyticus</em>, results in significant economic loss. This study aimed to understand the immune response of crayfish to bacterial infection by comparing and analyzing transcriptome data of hepatopancreatic tissue from <em>P. clarkii</em> challenged with <em>V. parahaemolyticus</em> or treated with PBS. Physiological indices (TP, Alb, ACP, and AKP) were analyzed, and tissue sections were prepared. After assembling and annotating the data, 18,756 unigenes were identified. A comparison of the expression levels of these unigenes between the control and <em>V. parahaemolyticus</em> groups revealed 4037 DEGs, with 2278 unigenes upregulated and 1759 downregulated in the <em>V. parahaemolyticus</em> group. GO (Gene Ontology) enrichment analysis shows that the DGEs are mainly enriched in cellular anatomical activity, bindinga and cellular process, enrichment analysis of KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways showed that DGEs were mainly enriched in Base excision repair, Phagosome and Longevity regulating pathway. At the same time, lysosome was also enriched. The phagosome and lysosome pathways play a crucial role in the immune defense of crayfish against <em>V. parahaemolyticus</em> injection that will be highlighted. In addition, the expression levels of six selected immune-related DEGs were measured using qRT-PCR, which validated the results of RNA-seq analysis. This study provides a new perspective on the immune system and defense mechanisms of <em>P. clarkii</em> and a valuable foundation for further investigation of the molecular immune mechanisms of this species.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and expression patterns of the Fox gene family under heat and cold stress in Manila clam Ruditapes philippinarum based on genome-wide identification","authors":"Tao Liu ,&nbsp;Yue Zhang ,&nbsp;Hongtao Nie ,&nbsp;Jingxian Sun ,&nbsp;Xiwu Yan","doi":"10.1016/j.cbd.2024.101313","DOIUrl":"10.1016/j.cbd.2024.101313","url":null,"abstract":"<div><p>In this study, the Fox gene family of <em>Ruditapes philippinarum</em> was identified by bioinformatics analysis and genome data. The results showed that a total of 21 Fox genes were identified in <em>R. philippinarum</em>, which were divided into 16 subfamilies, including two members of Foxa subfamily (<em>Foxa1, Foxa2</em>), three members of Foxl subfamily (<em>Foxl1b, Foxl1a, FOXL2</em>), three members of Foxn subfamily (<em>FOXN3, FOX4A, Foxn4b</em>) and one member of other families. The chromosome distribution, domains, conserved motifs, introns, exons and protein tertiary structures of these 21 <em>Fox</em> genes were predicted. By analyzing the RNA-seq data of <em>R. philippinarum</em>, it was found that the Fox gene family was differentially expressed in different tissues, different developmental stages and under heat and cold stress. Most of <em>Fox</em> genes were highly expressed in four tissues: labial palp, gonad, gill and foot. Most of the <em>Fox</em> genes were highly expressed in blastula stage. Most of the <em>Fox</em> genes were highly expressed in high temperature group of two populations, and <em>Foxo</em>, <em>FOXG1</em> were highly expressed in low temperature group. In addition, qPCR showed that the expression levels of <em>Foxo</em> and <em>Foxj1b</em> genes increased significantly under acute cold stress. Therefore, we speculate that <em>Fox</em> genes may play important roles in embryo development and the temperature stress of <em>R. philippinarum</em>, and this study provides a basis for further exploring the molecular mechanism of low temperature tolerance mediated by <em>Fox</em>.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of mRNA and miRNA reveals new insights into the regulatory mechanisms of Anadara granosa responses to heat stress","authors":"Xin He ,&nbsp;Yushan Liao ,&nbsp;Yiping Shen ,&nbsp;Junfa Shao ,&nbsp;Shi Wang ,&nbsp;Yongbo Bao","doi":"10.1016/j.cbd.2024.101311","DOIUrl":"10.1016/j.cbd.2024.101311","url":null,"abstract":"<div><p>Temperature fluctuations resulting from climate change and global warming pose significant threats to various species. The blood clam, <em>Anadara granosa</em>, a commercially important marine bivalve, predominantly inhabits intertidal mudflats that are especially susceptible to elevated temperatures. This vulnerability has led to noticeable declines in the survival rates of <em>A. granosa</em> larvae, accompanied by an increase in malformations. Despite these observable trends, there is a lack of comprehensive research on the regulatory mechanisms underlying <em>A. granosa</em>'s responses to heat stress. In this study, we examined the survival rates of <em>A. granosa</em> under varying high temperature conditions, selecting 34 °C as heat stress temperature. Enzyme activity assays have shed light on <em>A. granosa</em>'s adaptive response to heat stress, revealing its ability to maintain redox balance and transition from aerobic to anaerobic metabolic pathways. Subsequently, mRNA and miRNA transcriptome analyses were conducted, elucidating several key responses of <em>A. granosa</em> to heat stress. These responses include the upregulation of transcription and protein synthesis, downregulation of proteasome activity, and metabolic pattern adjustments. Furthermore, we identified miRNA-mRNA networks implicated in heat stress responses, potentially serving as valuable candidate markers for <em>A. granosa</em>'s heat stress response. Notably, we validated the involvement of agr-miR-3199 in <em>A. granosa</em>'s heat stress response through its regulation of the target gene <em>Foxj1</em>. These findings not only deepen our understanding of the molecular mechanisms underlying the blood clam's response to heat stress but also offer valuable insights for enhancing heat stress resilience in the blood clam aquaculture industry. Moreover, they contribute to improved cultivation strategies for molluscs in the face of global warming and have significant implications for the conservation of marine resources and the preservation of ecological balance.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the lipid profile of Anopheles stephensi mosquitoes across developmental life stages","authors":"Gabriela Ramirez ,&nbsp;Corey Broeckling ,&nbsp;MaKala Herndon ,&nbsp;Madison Stoltz ,&nbsp;Gregory D. Ebel ,&nbsp;Karen M. Dobos","doi":"10.1016/j.cbd.2024.101312","DOIUrl":"10.1016/j.cbd.2024.101312","url":null,"abstract":"<div><p>Holometabolous insects undergo a distinct transition in their development, tightly correlated with shifting feeding patterns from larval stages and some adult phases to non-feeding phases as pupae and during other adult phases. Furthermore, the intricate life cycle of mosquitoes involves a sequence of developmental stages influenced by aquatic and terrestrial factors, demanding precise energy resource orchestration. Lipids serve multifaceted roles, encompassing energy storage, membrane structure, and participation in signal transduction and molecular recognition processes. A significant gap in the current research landscape is the need for a comprehensive study exploring the lipid repertoire throughout the developmental stages of <em>Anopheles stephensi</em> mosquitoes. We undertook an analysis of the <em>An. stephensi</em> metabolome across all life stages. We hypothesized that <em>An. stephensi</em> mosquitoes will have unique lipid metabolite markers for each life stage. A specific extraction and LC-MS based lipidomic approach was used to test this hypothesis. Our findings demonstrated that our methods were successful, with lipids comprising 62.15 % of the analyzed metabolome. Additionally, phospholipids (PL), lysophospholipids (LPL), sphingomyelin (SM), and triglycerides (TG) were abundant and dynamic across all life stages. Interestingly, comparison between the L1 and L2 lipidome revealed a dominant pattern of specific TGs in decreased abundance between these two life stages. Lastly, 20-hydroxyecdysone (20E), was found to be present in similar abundance across all 4 larval stages. These data indicate that there may be lipid metabolome pathways serving unique roles during mosquito development that may be used to explore laboratory management of colonies, parasite resistance, and environmental adaptation.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1744117X24001254/pdfft?md5=53819375cc9c819d5d4f09fb40eec489&pid=1-s2.0-S1744117X24001254-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142045027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of gap junction gene family and their expression profiles under low temperature stress in noble scallop Chlamys nobilis","authors":"Changping Xu ,&nbsp;Xuanbing Wu ,&nbsp;Jiale Qiu ,&nbsp;Jianming Ye ,&nbsp;Qing Lin ,&nbsp;Jingwen Deng ,&nbsp;Yetao Zeng ,&nbsp;Weili Wang ,&nbsp;Hongkuan Zhang ,&nbsp;Huaiping Zheng","doi":"10.1016/j.cbd.2024.101310","DOIUrl":"10.1016/j.cbd.2024.101310","url":null,"abstract":"<div><p>Gap junctions, formed by gap junction proteins (GJ), play crucial roles in cell signaling and immune responses. The structure and function of the GJ from vertebrates (called connexins) have been extensively studied. However, little is known about the proteins forming gap junctions in invertebrates (called innexins). In this study, 14 <em>GJ</em> genes of <em>Chlamys nobilis</em> were identified. GJ proteins are mainly distributed on the plasma membrane, and all proteins are hydrophilic Phylogenetic tree analysis showed that the GJ proteins in <em>C. nobilis</em> were distantly related to those in vertebrates but closely related to those in invertebrates. Conserved motifs analysis of these GJ proteins in <em>C. nobilis</em> identified to have 10 conserved motifs, similar to gap junction proteins in other bivalves. Moreover, expression profiles of <em>CnGJ</em> genes under chronic and acute low temperature stress were also investigated. Results showed that chronic low temperature stress had a significant effect on the expression levels of <em>CnGJ</em> genes, and the expression profiles of <em>CnGJ</em> genes showed significantly variation under acute low temperature stress. All these results indicated that <em>CnGJ</em> genes play important roles in environmental adaptation in scallops. The present study initially elucidated the function of gap junction genes in noble scallop <em>C. nobilis</em>, which provides new insights into the <em>GJ</em> genes in mollusks and will help us better understand their roles in environmental stress in scallops.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141954056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics reveals the ecological and biological functions of Enterococcus mundtii in the intestine of lepidopteran insects","authors":"Guannan Li ,&nbsp;Meihong Wu ,&nbsp;Yi Xiao ,&nbsp;Yujie Tong ,&nbsp;Sheng Li ,&nbsp;Heying Qian ,&nbsp;Tianfu Zhao","doi":"10.1016/j.cbd.2024.101309","DOIUrl":"10.1016/j.cbd.2024.101309","url":null,"abstract":"<div><p>Insect guts offer unique habitats for microbial colonization, with gut bacteria potentially offering numerous benefits to their hosts. Although <em>Enterococcus</em> has emerged as one of the predominant gut commensal bacteria in insects, its establishment in various niches within the gut has not been characterized well. In this study, <em>Enterococcus mundtii</em> was inoculated into the silkworm (<em>Bombyx mori</em> L.) to investigate its biological functions. Genome-based analysis revealed that its successful colonization is related to adherence genes (<em>ebpA</em>, <em>ebpC</em>, <em>efaA</em>, <em>srtC</em>, and <em>scm</em>). This bacterium did not alter the activities of related metabolic enzymes or the intestinal barrier function. However, significant changes in the gene expressions levels of <em>Att2</em>, <em>CecA</em>, and <em>Lys</em> suggest potential adaptive mechanisms of host immunity to symbiotic <em>E. mundtii</em>. Moreover, 16S metagenomics analysis revealed a significant increase in the relative abundance of <em>E. mundtii</em> in the intestines of silkworms following inoculation. The intestinal microbiome displayed marked heterogeneity, an elevated gut microbiome health index, a reduced microbial dysbiosis index, and low potential pathogenicity in the treatment group. Additionally, <em>E. mundtii</em> enhanced the breakdown of carbohydrates in host intestines. Overall, <em>E. mundtii</em> serves as a beneficial microbe for insects, promoting intestinal homeostasis by providing competitive advantage. This characteristic helps <em>E. mundtii</em> dominate complex microbial environments and remain prevalent across Lepidoptera, likely fostering long-term symbiosis between the both parties. The present study contributes to clarifying the niche of <em>E. mundtii</em> in the intestine of lepidopteran insects and further reveals its potential roles in their insect hosts.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}