Integrated multi-omics analysis and RNAi reveal Toll signaling pathway and melanization as key defenses in Ostrinia furnacalis against Meteorus pulchricornis parasitism

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-05-17 DOI:10.1016/j.cbd.2025.101537

Ziyuan Tang , Gui Gao , Zhilin Xia , Changli Yang , Changyu Zhang

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Abstract

Ostrinia furnacalis, a worldwide agricultural pest, can be effectively controlled using the endoparasitic wasp Meteorus pulchricornis. However, the molecular mechanisms underlying the host immune response to parasitism remain poorly understood. This study aimed to explore the possible immune molecular mechanisms of O. furnacalis under parasitism by M. pulchricornis for 24 and 48 h (P24 and P48). Overall, 97 differentially expressed genes (DEGs) and 18 differentially expressed proteins (DEPs) associated with immunity were identified, including pattern-recognition receptors (PRRs), antimicrobial peptides (AMPs), antioxidant enzymes, Toll signaling pathway and melanization components. Three Toll signaling pathway downstream genes OfMyD88, OfGlov, and OfLys were chosen for functional analysis by RNA interference (RNAi). The results showed a notable increase in parasitism rate (7–9 %) in immunocompromised hosts and a significant decrease in survival rates (23–27 %), demonstrating that OfMyD88, OfGlov, and OfLys play crucial roles in the parasitic stress response and larvae survival. Our findings reveal novel insights into the immune molecular mechanisms of O. furnacalis and highlight potential targets for enhancing parasitic wasp-based biocontrol strategies.

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综合多组学分析和RNAi分析发现，Toll信号通路和黑化作用是玉米螟对pulchricornis寄生的关键防御机制

狐齿螟是一种世界性的农业害虫，利用内寄生黄蜂可有效防治狐齿螟。然而，宿主对寄生免疫反应的分子机制仍然知之甚少。本研究旨在探讨白斑棘球蚴寄生24和48 h （P24和P48）后，furnacalis可能的免疫分子机制。总共鉴定出97个与免疫相关的差异表达基因（DEGs）和18个差异表达蛋白（DEPs），包括模式识别受体（PRRs）、抗菌肽（AMPs）、抗氧化酶、Toll信号通路和黑色素化成分。选择Toll信号通路下游基因OfMyD88、OfGlov和OfLys进行RNA干扰（RNAi）功能分析。结果表明，免疫功能低下寄主的寄生率显著升高（7 - 9%），存活率显著降低（23 - 27%），表明OfMyD88、OfGlov和offlys在寄生应激反应和幼虫存活中起重要作用。我们的研究结果揭示了对furnacalis免疫分子机制的新见解，并强调了加强寄生蜂生物防治策略的潜在靶点。

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.