Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献_第3页

Melanin metabolism mediated via tyrosinase protein involved in red shell coloration of hard clam, Meretrix meretrix 酪氨酸酶蛋白介导的黑素代谢与硬蛤红壳着色的关系

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-27 DOI: 10.1016/j.cbd.2025.101619

Lu-lu Fu , He-ming Shi , Ying-hui Dong , Zhi-hua Lin , Yong-bo Bao , Hai-min Chen , Han-han Yao

{"title":"Melanin metabolism mediated via tyrosinase protein involved in red shell coloration of hard clam, Meretrix meretrix","authors":"Lu-lu Fu , He-ming Shi , Ying-hui Dong , Zhi-hua Lin , Yong-bo Bao , Hai-min Chen , Han-han Yao","doi":"10.1016/j.cbd.2025.101619","DOIUrl":"10.1016/j.cbd.2025.101619","url":null,"abstract":"<div><div>Molluscan shell color polymorphism is influenced by a variety of factors, and endocrine pigments secreted by mollusks play a crucial role in determining shell coloration. The hard clam (<em>Meretrix meretrix</em>) exhibits one of the most extensive variations in shell color among molluscan species, and the mechanism underlying its shell pigmentation remains largely unexplored. The stable inheritance of red shell color in a newly identified variant of <em>M. meretrix</em>, offers a valuable model for investigating the genetic basis of this phenotype. In this study, we conducted transcriptomic and proteomic analyses of the mantle tissues from both red and white shell clams to elucidate the molecular mechanisms responsible for the observed differences in shell color. Our results revealed that genes and proteins with significant differential expressions were primarily associated with carotenoid metabolism, melanin synthesis, biomineralization, and substance transport. Notably, tyrosinase like protein (encoded by <em>Tyrl</em>), a key enzyme involved in melanin synthesis, exhibited significantly higher transcript and protein levels in red shell clams compared to white shell clams. Consequently, we measured the melanin content and analyzed the expression profile of <em>Mm-Tyrl</em> using qPCR, Western blotting, and immunofluorescence techniques. These findings suggest that melanin and its related metabolic enzyme Mm-Tyrl may serve as novel regulators of red shell pigmentation in <em>M. meretrix</em>. The data presented herein provides new insights into the molecular mechanisms governing red shell coloration in clams and enhance our understanding of shell color polymorphisms in mollusks.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101619"},"PeriodicalIF":2.2,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-genome identification of SOD gene family revealed the expansion of SOD in Chinese mitten crab, Eriocheir sinensis SOD基因家族的全基因组鉴定揭示了中华绒螯蟹（Eriocheir sinensis）中SOD的扩增

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-27 DOI: 10.1016/j.cbd.2025.101618

Xinxin Chen , Maolei Wei , Qi Zhu , Xirui Zheng , Xiaowu Chen

{"title":"Whole-genome identification of SOD gene family revealed the expansion of SOD in Chinese mitten crab, Eriocheir sinensis","authors":"Xinxin Chen , Maolei Wei , Qi Zhu , Xirui Zheng , Xiaowu Chen","doi":"10.1016/j.cbd.2025.101618","DOIUrl":"10.1016/j.cbd.2025.101618","url":null,"abstract":"<div><div>Superoxide dismutase (<em>SOD</em>) genes are essential components of the antioxidant defense system in crustacean. However, <em>SOD</em> genes have not been identified in Chinese mitten crab (<em>Eriocheir sinensis</em>). In this study, 6 <em>SOD</em> genes including 5 <em>SOD1</em> and 1 <em>SOD2</em> genes were identified through a comparative analysis in <em>E. sinensis</em>. Phylogenetic analysis clustered all <em>SODs</em> including outgroups into three groups: <em>SOD1</em>, <em>SOD2</em>, and <em>SOD3</em>. Among these three groups, <em>SOD1</em> exhibited the highest number of genes, followed by <em>SOD2</em>. Chromosomal mapping revealed that <em>SOD1.1</em>, <em>SOD1.2</em>, <em>SOD1.4</em>, <em>SOD1.5</em> and <em>SOD2</em> were located on chromosomes (Chr52, Chr7, Chr41 and Chr19), while <em>SOD1.3</em> was distributed on scaffolds. Sequence characteristics analyses revealed that <em>SOD1</em> and <em>SOD2</em> had large sequence differences and different structure domain. Transcriptomic profiling showed tissue-specific expression patterns, with <em>SOD2</em> and <em>SOD1.5</em> highly expressed in the gill. In addition, <em>SOD2</em> exhibited a high expression level during the intermolt (stage C), pre-molt (stage D). These findings suggest that <em>SOD2</em> may play critical roles in oxidative stress management and molting regulation, providing valuable insights into the antioxidant mechanisms of crustaceans.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101618"},"PeriodicalIF":2.2,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular responses and mechanisms of the sea cucumber Apostichopus japonicus under prolonged hypoxic conditions 长时间缺氧条件下刺参的分子响应及其机制

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-26 DOI: 10.1016/j.cbd.2025.101623

Peng Liu , Shufeng Li , Jinge Yu , Qiwei Zuo , Jiaqi Liu , Jiayi Zhang , Haoran Xiao , Yongjie Wang , Lingshu Han , Chong Zhao , Jun Ding

{"title":"Molecular responses and mechanisms of the sea cucumber Apostichopus japonicus under prolonged hypoxic conditions","authors":"Peng Liu , Shufeng Li , Jinge Yu , Qiwei Zuo , Jiaqi Liu , Jiayi Zhang , Haoran Xiao , Yongjie Wang , Lingshu Han , Chong Zhao , Jun Ding","doi":"10.1016/j.cbd.2025.101623","DOIUrl":"10.1016/j.cbd.2025.101623","url":null,"abstract":"<div><div>In recent decades, hypoxia has become widespread in coastal waters. Research on the molecular response mechanisms of sea cucumbers (<em>Apostichopus japonicus</em>) under long-term hypoxic stress is limited. Consequently, an 18-day hypoxia experiment was conducted to examine the extreme tolerance. We simulated realistic conditions by establishing dissolved oxygen (DO) levels of 2.0, 3.0, 4.0, 5.0, 6.0, and 7.0 mg/L. The results showed that chronic hypoxia had detrimental effects on respiration, survival, and weight gain of sea cucumbers. The oxygen consumption rate of <em>A. japonicus</em> decreased as the DO level declined (<em>P</em> < 0.05). Survival rates were lowest at DO levels of 2.0 and 3.0 mg/L, with survival rates of 20 ± 5 % and 45.0 ± 6.8 %, respectively. Compared to the control group, sea cucumbers at 2.0 mg/L not only exhibited a higher mortality rate, but also experienced a significant reduction in body weight (<em>P</em> < 0.05), with a weight gain rate of −8.75 ± 0.61 %. At the molecular level, sea cucumbers downregulate the expression of <em>PFK</em> and <em>PDH</em> in long-term hypoxia, thereby constraining energy generation via glycolysis and the tricarboxylic acid (TCA) cycle, and prompting <em>A. japonicus</em> to increasingly depend on augmented fatty acid β-oxidation to meet its energy requirements. Furthermore, cortisol secretion enhances carbohydrate metabolism, which in turn helps alleviate oxidative stress. Concurrently, <em>A. japonicus</em> increased the expression of <em>GCL</em> and <em>MGST</em>, thereby enhancing their cellular antioxidant and immune functions by regulating glutathione metabolism and other amino acids. This study enhances the understanding of the molecular mechanisms by which <em>A. japonicus</em> responds to prolonged hypoxia.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101623"},"PeriodicalIF":2.2,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new insight on the application of phytosterols in the aquatic feed: Improve the growth and intestinal health of largemouth bass (Micropterus salmoides) fed the high starch diet 植物甾醇在水产饲料中应用的新认识：促进高淀粉饲料喂养的大口黑鲈生长和肠道健康

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-25 DOI: 10.1016/j.cbd.2025.101617

Yuxuan Ma , Haisong Shi , Zhongchao Pan , Yijing Liu , Minhao Xia , Paihuai Ouyang , Fenggang Sun , Kangyuan Qu , Yucheng Liu , Junming Deng , Hongyu Liu , Beiping Tan , Shiwei Xie

{"title":"A new insight on the application of phytosterols in the aquatic feed: Improve the growth and intestinal health of largemouth bass (Micropterus salmoides) fed the high starch diet","authors":"Yuxuan Ma , Haisong Shi , Zhongchao Pan , Yijing Liu , Minhao Xia , Paihuai Ouyang , Fenggang Sun , Kangyuan Qu , Yucheng Liu , Junming Deng , Hongyu Liu , Beiping Tan , Shiwei Xie","doi":"10.1016/j.cbd.2025.101617","DOIUrl":"10.1016/j.cbd.2025.101617","url":null,"abstract":"<div><div>This study investigated the effects of phytosterols (PS) supplementation in high-starch diets on growth, antioxidant capacity, muscle/intestinal morphology, and gut microbiota in largemouth bass (<em>Micropterus salmoides</em>). A total of 960 fish (11.14 ± 0.48 g) were divided into six groups (Control, 8 % starch; HC, 13 % starch; HCP1-HCP4: HC + 0.025 %/0.05 %/0.075 %/0.1 % PS) for an 8-week feeding trial. Compared to the HC diet, dietary supplementation of 0.05 % PS significantly enhanced the growth and anti-oxidative ability of largemouth bass, as well as reduced MDA concentration and suppressed digestive enzyme activities. PS supplementation improved intestinal health by increasing muscle fiber density and intestinal fold width, also up-regulated the expression of <em>bcl-2</em>, <em>claudin-1</em>, <em>claudin-4</em>, and <em>mef2d</em> while downregulated the expression of <em>murf1</em>, <em>atrogin1</em>, and <em>mstn</em> (<em>P</em> < 0.05). PS modulated apoptosis by elevated BAD protein fluorescence intensity while reduced BCL-2 and CASPASE-3 protein fluorescence intensity (<em>P</em> < 0.05). Additionally, compared to HC diet, dietary supplementation of 0.05 % PS increased Firmicutes abundance and decreased Proteobacteria abundance. These findings demonstrated that 0.05 % PS supplementation in HC diets improved growth performance, muscle quality, antioxidant capacity, intestinal integrity, and beneficial microbiota composition of <em>largemouth bass</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101617"},"PeriodicalIF":2.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin enhances antioxidant capacity and reproductive performance in daphnia pulex 槲皮素提高水蚤抗氧化能力和繁殖性能

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-25 DOI: 10.1016/j.cbd.2025.101625

Chenxi Zhu , Yanxia Guo , Mingming Han , Qichen Jiang

{"title":"Quercetin enhances antioxidant capacity and reproductive performance in daphnia pulex","authors":"Chenxi Zhu , Yanxia Guo , Mingming Han , Qichen Jiang","doi":"10.1016/j.cbd.2025.101625","DOIUrl":"10.1016/j.cbd.2025.101625","url":null,"abstract":"<div><div>Quercetin is a widely distributed flavonoid found in fruits and vegetables, known for its diverse biological effects. In this study, neonatal <em>Daphnia pulex</em> (within 24 h of birth) were exposed to varying concentrations of quercetin (0, 1, 2.5, 5, and 10 mg/L). At high concentrations, quercetin induced morphological deformities, including concavity on the dorsal surface, which appeared earlier in treated individuals compared to controls. Conversely, low concentrations of quercetin extended the average lifespan, increased molting frequency, and significantly enhanced the net reproduction rate and generation cycle. In contrast, high concentrations significantly reduced both molting frequency and net reproduction rate. Regarding metabolic and antioxidant enzyme activities, quercetin exposure decreased triglyceride (TG) levels. Low concentrations significantly downregulated the activities of acid phosphatase (ACP) and alkaline phosphatase (AKP), while all tested concentrations significantly reduced amylase (AMS) activity. Catalase (CAT) and glutathione (GSH) levels initially increased and then declined with rising quercetin concentrations, a pattern also observed for superoxide dismutase (SOD) activity after 14 days of exposure. Transcriptomic analysis revealed that quercetin treatment significantly upregulated metabolic pathways, followed by cellular processes and genetic information processing. Additionally, quercetin enhanced oxidative phosphorylation and alleviated oxidative stress. These findings suggest that low concentrations of quercetin promote the growth and reproduction of <em>D. pulex</em>, regulates metabolic activity, and enhances antioxidant capacity.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101625"},"PeriodicalIF":2.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gonadal transcriptome profiling via RNA-seq during temperature-dependent sex determination and differentiation in the red-eared slider (Trachemys scripta elegans) 在红耳滑鼠（Trachemys scripta elegans）的温度依赖性性别决定和分化过程中，通过RNA-seq进行性腺转录组分析

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-23 DOI: 10.1016/j.cbd.2025.101621

Jiawei Fan , Fang Li , Lingyuan Lou , Baoyou Huang , Qiran Chen , Wei Sun , Chutian Ge , Zongji Wang

{"title":"Gonadal transcriptome profiling via RNA-seq during temperature-dependent sex determination and differentiation in the red-eared slider (Trachemys scripta elegans)","authors":"Jiawei Fan , Fang Li , Lingyuan Lou , Baoyou Huang , Qiran Chen , Wei Sun , Chutian Ge , Zongji Wang","doi":"10.1016/j.cbd.2025.101621","DOIUrl":"10.1016/j.cbd.2025.101621","url":null,"abstract":"<div><div>In species with temperature-dependent sex determination (TSD), incubation temperature during a critical embryonic window directs gonadal fate, yet the underlying molecular mechanisms remain poorly understood. Here, we performed transcriptome sequencing of embryonic gonads from the red-eared slider turtle (<em>Trachemys scripta elegans</em>) incubated under male- and female-producing temperatures (MPT and FPT). Our analyses revealed numerous stage-specific differentially expressed genes and identified key candidates consistently upregulated under MPT or FPT conditions. Gene co-expression network analysis highlighted <em>DHRS11</em> as a potential thermosensitive hub gene at MPT, and <em>ATP2B4</em>, a calcium transporter, as a hub under FPT, implicating calcium signaling in early sex determination. In addition, we uncovered widespread alternative splicing, including intron retention in the chromatin modifiers <em>JARID2</em> and <em>KDM6B</em>, with <em>CIRBP</em>, a cold-inducible RNA-binding protein, as a potential upstream regulator. These findings suggest a role for transcriptional and post-transcriptional mechanisms, including calcium signaling and temperature-responsive splicing, in mediating TSD in reptiles.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101621"},"PeriodicalIF":2.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Untargeted metabolomics reveals compositional alterations in the muscle tissue of hybrid crucian carp 非靶向代谢组学揭示了杂交鲫鱼肌肉组织的组成变化

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-23 DOI: 10.1016/j.cbd.2025.101620

Weiling Qin , Leli He , Jisen Su , Tao Dai , Jiamin Pi , Yuling Zhou , Yi Zhang , Bohua Wang , Song Lei , Xianghong Li , Siyang He , Xiaolong Zhou , Xiangyu Zhao , Yang Huo , Minglin Dong , Huan Zhong , Yi Zhou

{"title":"Untargeted metabolomics reveals compositional alterations in the muscle tissue of hybrid crucian carp","authors":"Weiling Qin , Leli He , Jisen Su , Tao Dai , Jiamin Pi , Yuling Zhou , Yi Zhang , Bohua Wang , Song Lei , Xianghong Li , Siyang He , Xiaolong Zhou , Xiangyu Zhao , Yang Huo , Minglin Dong , Huan Zhong , Yi Zhou","doi":"10.1016/j.cbd.2025.101620","DOIUrl":"10.1016/j.cbd.2025.101620","url":null,"abstract":"<div><div>Fish breeding, including distant hybridization and backcrossing, can reinforce fish growth performance and flesh quality. Using the intercrossing and backcrossing, two improved crucian carps, WR1 and WR2, were generated with a fast growth rate and high-quality flesh. The present study focused on the metabolomic profiles of two genetically improved fish species and investigated the mechanisms of advanced traits underlying metabolic changes by comparing them to their original parents. By comparing WR1 with its parents, 65 differentially expressed metabolites (DEMs) showed significantly higher expression levels in both parental species, whereas 281 DEMs exhibited significantly lower expression levels in both. Among them, LysoPC 18:3, deoxycholic acid, and uric acid were significantly higher, whereas inosine was significantly lower in WR1. In WR2, 75 metabolites were significantly higher in the parents than in WR2, while 413 metabolites were significantly lower in the parents than in WR2. <span>l</span>-Carnitine, creatine, choline, and seven di−/tripeptides were upregulated in WR2. The higher levels of creatine in the muscle of WR2 compared to WR1 and the Weighted Gene Co-expression Network Analysis (WGCNA) results suggest that creatine is the key node that regulates a module containing 72 metabolites. The concentration of creatine in WR2 reached to 28.58 ± 3.01 μmol/g indicating the high concentration of creatine in WR2 was related with its growth performance and high-quality flesh. In conclusion, this study provides the first clues for genetic breeding to reconstruct the metabolic profiles of crucian carp, implying a metabolic mechanism for reinforcing growth performance and flesh quality.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101620"},"PeriodicalIF":2.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of lipid metabolism in Rana chensinensis tadpoles under lead (Pb) stress 铅胁迫下中国林蛙蝌蚪脂质代谢的响应

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-22 DOI: 10.1016/j.cbd.2025.101622

Xiaoli Liu , Ying Liu , Xiuling Song , Hongyuan Wang , Lihong Chai

{"title":"Responses of lipid metabolism in Rana chensinensis tadpoles under lead (Pb) stress","authors":"Xiaoli Liu , Ying Liu , Xiuling Song , Hongyuan Wang , Lihong Chai","doi":"10.1016/j.cbd.2025.101622","DOIUrl":"10.1016/j.cbd.2025.101622","url":null,"abstract":"<div><div>Lead (Pb) is a toxic contaminant ubiquitously present in aquatic ecosystems. Unfortunately, studies on the effects of Pb on lipid metabolism and gene expression in amphibians are limited. Here, lipidomic and hepatic transcriptomic analyses were performed on <em>Rana chensinensis</em> tadpoles exposed to 20 and 200 μg/L Pb. Our data demonstrated that phosphatidylcholine (PC) species were significantly reduced in the Pb20 group and significantly increased in the Pb200 group. The decrease in PC content may be related to membrane damage in tadpoles. Meanwhile, liver transcriptome profiling revealed that differentially expressed genes induced by different concentrations of Pb treatment were simultaneously enriched in <em>Staphylococcus aureus</em> infection pathway, with significantly increased expression of <em>FB</em>, <em>C3</em>, and <em>MHC-II</em> in the Pb20 group and <em>C3AR1</em> in the Pb200 group. Moreover, Pb exposure resulted in liver damage (e.g., lipid droplet accumulation) and significantly reduced body size in tadpoles at metamorphosis, which may have increased their susceptibility to mortality. This study is the first to investigate the deleterious effects of Pb on lipid metabolism and hepatic gene expression in tadpoles, shedding light on the potential molecular mechanisms of Pb toxicity in amphibians.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101622"},"PeriodicalIF":2.2,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic profiling of Ruditapes philippinarum gills exposed to triazophos: Insights into oxidative stress, cytoskeletal alterations, protein homeostasis and energy metabolism enhancement 暴露于三唑磷的菲律宾鲷鱼鳃的蛋白质组学分析：氧化应激，细胞骨架改变，蛋白质稳态和能量代谢增强的见解

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-20 DOI: 10.1016/j.cbd.2025.101613

Jingjing Ma , Fei Tian , Wei Sun , Zhe Zhang , Haigang Chen , Linbao Zhang

{"title":"Proteomic profiling of Ruditapes philippinarum gills exposed to triazophos: Insights into oxidative stress, cytoskeletal alterations, protein homeostasis and energy metabolism enhancement","authors":"Jingjing Ma , Fei Tian , Wei Sun , Zhe Zhang , Haigang Chen , Linbao Zhang","doi":"10.1016/j.cbd.2025.101613","DOIUrl":"10.1016/j.cbd.2025.101613","url":null,"abstract":"<div><div>The extensive utilization of triazophos (TP) caused a threat to aquatic organisms, particularly the non-target organisms such as clams. The current research aimed to evaluate the impact of sub-lethal concentration of TP on Malina clam <em>Ruditapes philippinarum</em>. Clams were subjected to a TP concentration at 25 μg/L (equivalent to 2 % of the 96 h LC<sub>50</sub> value) for a period of 7 d, then the proteomic alterations, as well as the activities and gene expression levels of antioxidant enzymes in the clam gills were examined. Proteomics revealed that TP exposure induced 62 differentially abundant proteins (DAPs) in the gills, including 51 with higher abundance and 11 lower abundance. Combined with the findings of enzyme activities (contents) and gene expression analysis, we observed significant variations in the abundance of proteins involved in oxidative stress, cytoskeletal regulation, protein homeostasis, and energy metabolism pathways in the gills under 7 d of TP stress. This suggested that at low concentration, TP might activate stress response pathways or cellular defense strategies, thereby leading to the upregulation of certain proteins.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101613"},"PeriodicalIF":2.2,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal profiling of novel and known miRNAs during cold acclimation in Aedes aegypti 埃及伊蚊冷驯化过程中新型和已知mirna的热谱分析

IF 2.2 2区生物学

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-08-20 DOI: 10.1016/j.cbd.2025.101614

Ella H.M. De Nicola , Kyle K. Biggar , Heath A. MacMillan

{"title":"Thermal profiling of novel and known miRNAs during cold acclimation in Aedes aegypti","authors":"Ella H.M. De Nicola , Kyle K. Biggar , Heath A. MacMillan","doi":"10.1016/j.cbd.2025.101614","DOIUrl":"10.1016/j.cbd.2025.101614","url":null,"abstract":"<div><div>The disease vector, <em>Aedes aegypti</em>, can better survive low temperature exposure following cold acclimation. This plasticity may be contributing to this species' recent range expansion. However, the molecular mechanisms behind this ability remain unexplored. MicroRNAs (miRNAs) can drive rapid changed in gene expression and are known to regulate thermal plasticity in other animals. We used a bioinformatic approach to identify three novel miRNAs in <em>Ae. aegypti</em>. After acclimating adult female <em>Ae. aegypti</em> to cold (15 °C) and warm (25 °C) conditions, we used RT-qPCR to perform differential expression analysis on these novel miRNAs and four other miRNAs downregulated during diapause in another mosquito species. Two novel miRNAs, aae-miR-novel2 and aae-miR-novel3, were upregulated in cold-acclimated individuals. Two described miRNAs associated with lipid metabolism and reproduction, aae-miR-8-3p and aae-miR-13b-3p, were downregulated in the cold-acclimated group, suggesting these processes are affected during cold acclimation. Temperature alone can alter miRNA target genes, and the thermodynamic parameters used to predict miRNA targets are typically established assuming body temperatures relevant to endotherms, rather than those applying to poikilothermic ectotherms. Here, we instead tested for cold-influenced targets of miRNAs of interest, predicted to only become thermodynamically stable at 15 °C. This approach provides insight into the putative roles that these novel miRNAs could play in cold acclimation. Many predicted targets were involved in processes associated with insect low temperature survival, like ion homeostasis and membrane stability, while others suggest roles for understudied processes. Our results suggest that miRNAs play a regulatory role in mosquito thermal plasticity that deserves further attention.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101614"},"PeriodicalIF":2.2,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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