Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Genome-wide identification and characterization of IGF/IGFR/IGFBP gene families in spotted sea bass and their expression profiles in response to environmental stress","authors":"Chong Zhang ,&nbsp;Xinlin Yang ,&nbsp;Yonghang Zhang ,&nbsp;Shaosen Yang ,&nbsp;Cong Liu ,&nbsp;Pengyu Li ,&nbsp;Lingyu Wang ,&nbsp;Xin Qi ,&nbsp;Kaiqiang Zhang ,&nbsp;Haishen Wen ,&nbsp;Yun Li","doi":"10.1016/j.cbd.2025.101575","DOIUrl":"10.1016/j.cbd.2025.101575","url":null,"abstract":"<div><div>Insulin-like growth factor (IGF) signaling pathway constitutes an ancient regulatory network essential for neuroendocrine functions, significantly influencing developmental processes, reproductive activities, and adaptation to environmental challenges. In this study, we detected 3 <em>IGF</em> genes, 3 <em>IGFR</em> genes and 11 <em>IGFBP</em> genes in spotted sea bass genome. The annotation accuracy and evolutionary conservation of identified genes were confirmed through phylogenetic, syntenic and copy number analyses. Molecular basis of gene functions, and protein interaction relationships were investigated by gene structure and protein-protein interaction (PPI) network analyses. Moreover, selective pressure analysis revealed that most genes primarily underwent purifying selection during evolution. Furthermore, RNA-Seq datasets were utilized to analyze tissue expression profiling for <em>IGF</em>/<em>IGFR</em>/<em>IGFBP</em> genes, along with documentation of their environmental stress-responsive transcriptional dynamics in targeted tissues. Our results demonstrated that <em>IGF</em>/<em>IGFR</em>/<em>IGFBP</em> genes were widely expressed in different tissues, with several genes exhibiting high expression levels in specific tissues. Additionally, the stress response mechanisms of IGF signaling system mainly involve IGFs functions regulated by IGFBPs and IGF-independent functions of IGFBPs. Notably, <em>igf1</em>, <em>igf2</em>, <em>igfbp1a</em>, <em>igfbp3b</em> and <em>igfbp4</em> were pinpointed as key functional genes of environmental challenge adaptation in this species. This investigation provides foundational insights into the <em>IGF</em>/<em>IGFR</em>/<em>IGFBP</em> gene families in spotted sea bass, delivering the first comprehensive genomic resource for these gene families while clarifying their functional contributions to environmental stress adaptation.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101575"},"PeriodicalIF":2.2,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic resources and transcriptomic evidence of thermal stress in the southern saucer scallop (Ylistrum balloti)","authors":"Sharon E. Hook ,&nbsp;Alistair J. Hobday ,&nbsp;Ryan J. Farr ,&nbsp;Jenny Su ,&nbsp;Leon Court ,&nbsp;Gunjan Pandey ,&nbsp;Rahul V. Rane ,&nbsp;Mervi Kangas ,&nbsp;Michel Bermudes ,&nbsp;Aisling Fontanini ,&nbsp;Thomas Walsh","doi":"10.1016/j.cbd.2025.101571","DOIUrl":"10.1016/j.cbd.2025.101571","url":null,"abstract":"<div><div>Marine heatwaves can lead to mass mortality events, affecting bivalves. The saucer scallop (<em>Ylistrum balloti</em>) is an important commercial species found in Western Australia (WA) and central Queensland. The largest fishery for this species in WA is at Shark Bay, on the central WA coastline. The fishery has been impacted by heatwaves in the recent past and has yet to fully recover. Whether thermal pressures are influencing the fishery's recovery is uncertain, as other factors, including larval advection, could also influence recovery. A functional genomics approach was used to address this uncertainty. Scallops were collected from multiple locations in Shark Bay with different water temperatures and from a small, more southerly fishery adjacent to Perth. A full genome was assembled. The genome contains comparatively few gene copies of heat shock protein 70, an important indicator of thermal stress, particularly from class 12, which has been highly duplicated in other heat tolerant bivalves. Transcriptomic profiles in scallops collected from the different locations were compared, with the greatest differences observed in scallops collected from an area of Shark Bay with the warmest water temperatures. Some transcripts with altered abundance encoded heat shock proteins, proteins involved in macromolecule repair, and those with a primary metabolism role. Although this study is a snapshot of a few individuals at a single time point, it adds to the weight of evidence that extreme heatwave events and climate change impacts the recruitment of this commercially important species.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101571"},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis of juvenile greater amberjack (Seriola dumerili) revealed the molecular responses of the kidney to chronic salinity stress","authors":"Mingzhu Chen ,&nbsp;Xiaoying Ru ,&nbsp;Yuhao Peng ,&nbsp;Xinyi Li ,&nbsp;Yang Huang ,&nbsp;Chunhua Zhu ,&nbsp;Guangli Li ,&nbsp;Hongjuan Shi","doi":"10.1016/j.cbd.2025.101572","DOIUrl":"10.1016/j.cbd.2025.101572","url":null,"abstract":"<div><div>Salinity is an important environmental factor, and fluctuations in saline-alkali levels that exceed the osmoregulatory capacity of fish may have profound effects on various physiological functions of teleosts. The greater amberjack is highly valued commercially owing to its rapid growth and excellent meat quality. In this study, the juvenile greater amberjack were reared for 30 days under optimal salinity (30 ppt) as well as hypo- (20 ppt) and hyper-salinity (40 ppt) conditions. Histologically, the structure of the kidney was damaged under chronic salinity stress, characterized by hypertrophy at the edges of the columnar epithelial cells in the renal tubules, while the central side was tightened. Through comparative transcriptome analysis of the kidney, a total of 1103, 51, and 1711 differentially expressed genes (DEGs) were identified in the K20 vs. K30 (686 up- and 417 down-regulated), K40 vs. K30 (14 up- and 37 down-regulated), and K20 vs. K40 (1170 up- and 541 down-regulated) salinity stress groups, respectively. Certain DEGs enriched in the cell cytoskeleton (<em>LOC111217621</em>, <em>tubb5</em>, and <em>LOC111234965</em>) and cell apoptosis (<em>tnfrsfa</em>, <em>LOC111218420</em>, <em>LOC111223891</em>, <em>LOC111223286</em>, and <em>bcl2</em>) were identified by kidney transcriptome analysis in response to salinity stress. Furthermore, DEGs associated with lipid and carbohydrate metabolism (<em>elovl6l</em>, <em>acsl4</em>, <em>msmo1</em>, <em>pck1</em>, <em>g6pc1</em>, <em>pfkfb3</em>, and <em>ldha</em>), ion transport (<em>kcnk5</em>, <em>slc4a4</em>, <em>slc9a3</em>, <em>slc41a1</em>, and <em>slc22a2</em>), and immune response (<em>nfkbiaa</em>, <em>nfkbia</em>, and <em>LOC111231462</em>) were identified. These findings suggest that the cytoskeleton was damaged, along with variations in ion transport, lipid and carbohydrate metabolism, and immune responses to salinity stress. The current findings enhance our understanding of the physiological responses of greater amberjack to salinity stress, which could be beneficial in developing strategies to optimize the aquaculture and artificial breeding of this species in environments characterized by fluctuating salinity patterns.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101572"},"PeriodicalIF":2.2,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome profile analysis of mucosal immune responses in the gill of Takifugu rubripes infected with Vibrio harveyi","authors":"Dong Sheng ,&nbsp;Hongli Ma ,&nbsp;Xu Ren,&nbsp;Yuxiao Liu,&nbsp;Xiaolong Ding,&nbsp;Qinghua Yu,&nbsp;Weijia Zhou,&nbsp;Shigen Ye,&nbsp;Zhixin Guo","doi":"10.1016/j.cbd.2025.101570","DOIUrl":"10.1016/j.cbd.2025.101570","url":null,"abstract":"<div><div><em>Vibrio harveyi</em> is the main pathogen causing significant economic losses in <em>Takifugu rubripes</em> farming. The early immune response to <em>V. harveyi</em> infection in <em>T. rubripes</em> was investigated using Illumina NovaSeq 6000 technology. A total of 4720 differentially expressed genes were identified in the gill transcriptome at 0, 3, and 24 h post-infection compared to the control group. Pathways associated with mucosal immunity, such as cytokine-cytokine receptor interaction, cell adhesion molecules (CAMs), and the NOD-like receptor signaling pathway, were significantly enriched. Specifically, the results implied the cytokine-cytokine receptor interaction pathway is crucial in mediating immune responses. The cooperative interplay between Ccr7 and Cxcl13 may enhance pathogen resistance during the early mucosal immune phase in <em>T. rubripes</em>. These findings provide valuable insights into the early mucosal immune response to <em>V. harveyi</em> infection in <em>T. rubripes</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101570"},"PeriodicalIF":2.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profile of Lutraria sieboldii larvae: Insights into the molecular mechanisms of attachment and metamorphosis","authors":"Taiyue Xu ,&nbsp;Zhen Zhao ,&nbsp;Jie Zou ,&nbsp;Zhuocheng Wu ,&nbsp;Jiamin Xing ,&nbsp;Congyan Yu ,&nbsp;Lei Bao ,&nbsp;Xiaoke Wu ,&nbsp;Xin Liu ,&nbsp;Chong Li ,&nbsp;Yongqiang Zhang ,&nbsp;Huijing Peng ,&nbsp;Ying Pan ,&nbsp;Peng Zhu","doi":"10.1016/j.cbd.2025.101569","DOIUrl":"10.1016/j.cbd.2025.101569","url":null,"abstract":"<div><div>Metamorphosis, the crucial transition from planktonic to sessile life stages, marks a key developmental milestone in marine bivalves, particularly for commercially valuable species like <em>Lutraria sieboldii</em>. We conducted a comprehensive transcriptomic analysis using RNA sequencing (RNA-seq) during the larval settlement stage to elucidate the molecular mechanisms underlying this ecologically and economically significant process. Our study generated 89.64 Gb of high-quality clean data (≥6.20 Gb per sample, Q30 ≥ 92.7 %), ensuring robust and reliable insights into gene expression dynamics. Through weighted gene co-expression network analysis (WGCNA), we identified the turquoise module as a key regulatory network strongly associated with settlement-related traits. This module was significantly enriched in critical biological processes, including mRNA splicing via spliceosome, pathways related to cytoskeletal organization and calcium signaling, and C-type lectin receptor signaling, which are crucial for substrate interaction and tissue remodeling. Notably, hub genes within this module included essential components of the spliceosome (<em>SF3B1</em>, <em>RBM8A</em>), calcium signaling regulators (<em>CALM3</em>), and cytoskeletal modulators (<em>ACTB</em>, <em>MFAP1</em>). Furthermore, transcriptional profiling revealed a significant upregulation of genes involved in inorganic ion transport and metabolism, cytoskeletal dynamics, protein turnover, and energy production. These results offer strong evidence for the coordinated regulation of molecular mechanisms during substrate recognition and tissue restructuring in L. <em>sieboldii</em> metamorphosis. As the first comprehensive transcriptomic resource describing larval settlement in this species, our study advances our understanding of conserved developmental pathways and suggests potential aquaculture strategies, such as optimizing calcium signaling or spliceosome activity to enhance larval settlement efficiency in hatchery settings.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101569"},"PeriodicalIF":2.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabonomic and microbiomic analysis reveals the effect of sargassum enzyme hydrolysate compound fish protein hydrolysate on the intestinal health in Pacific white shrimp (Penaeus vannamei)","authors":"Yi Luo,&nbsp;Jinqi Xu,&nbsp;Songming Chen,&nbsp;Qiang Chen,&nbsp;Runzhe Zhang,&nbsp;Jiangyang Yin,&nbsp;Tao Han,&nbsp;Jiteng Wang","doi":"10.1016/j.cbd.2025.101568","DOIUrl":"10.1016/j.cbd.2025.101568","url":null,"abstract":"<div><div>The investigation of geographical feed raw materials is crucial to maintain the sustainable development of aquaculture. Ensuring the intestinal health of aquatic animals is the key to improving the absorption and utilization rate of new protein sources. In the present study, a composite protein source derived from the sargassum enzyme-hydrolysate compound fish protein hydrolysate (“SFPH” in the experiment) was utilized to replace 0 %, 5 %, 10 %, 20 %, 30 %, and 40 % of fish meal protein, respectively. Pacific white shrimp (<em>Penaeus vannamei</em>) with an initial weight of 0.9 g was used as experimental model for an 8-week feeding trial. The results revealed that SFPH replacement reduced the width of intestinal microvilli, but the 5%SFPH group significantly increased intestinal wall thickness and microvilli height. Antioxidant indicators analysis revealed that when over 10 % of fish meal protein was substituted with SFPH, the total antioxidant capacity dramatically decreased. When SFPH replaced 40 % of fish meal protein, the mRNA expression levels of endoplasmic reticulum stress-related indicators (<em>bip</em> and <em>atf4</em>) significantly increased, as well as the genes associated with apoptosis (<em>jnk</em>, <em>caspase 8</em> and <em>caspase 3</em>). The analysis of intestinal microbiota showed that the 5%SFPH group significantly increased the richness of intestinal microbiota and significantly increased the abundance of beneficial bacteria (<em>Campilobacter</em>, <em>Patescibacter</em>, <em>Deferribacter</em> and <em>Halobacter</em>). The 40%SFPH group significantly increased the abundance of pathogenic bacteria (<em>Pseudomonas</em>, <em>Serratia</em>, <em>Rickettsia, Edwardsiella</em>, and <em>Veillonella</em>). A total of 201 differential relative abundance metabolites were detected by metabolomics, among which the contents of succinate, oxidized glutathione and reduced glutathione were reduced in the 40%SFPH group. The differential metabolites were mainly enriched in cysteine and methionine metabolism, biosynthesis of amino acid and glutathione metabolism. Based on the integrated analysis of metabolomics and intestinal microbiota, changes in signal pathways such as amino acid metabolism and glutathione synthesis may be the main reasons for the high proportion of SFPH disrupting intestinal health. This study provides in-depth data on the reaction mechanisms of shrimp to seaweed protein, and also provides new directions for the improvement of seaweed protein in the future.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101568"},"PeriodicalIF":2.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ocean acidity extremes retard shell formation of bivalve larvae: Insights from transcriptomics and lipidomics","authors":"Yang Xu ,&nbsp;Zhen Zhao ,&nbsp;Xin Luo ,&nbsp;Fortunatus Masanja ,&nbsp;Yuewen Deng ,&nbsp;Jonathan Y.S. Leung ,&nbsp;Liqiang Zhao","doi":"10.1016/j.cbd.2025.101566","DOIUrl":"10.1016/j.cbd.2025.101566","url":null,"abstract":"<div><div>In view of climate change and human activities, ocean acidity extreme (OAX) events have been increasingly reported worldwide over the last decades, which possibly retard the growth and development of marine organisms, particularly at their early life-history stages (e.g., embryos or larvae). Thus, understanding whether they can adjust to the sudden increase in seawater acidity has drawn growing attention. Using a commercially and ecologically important bivalve species (<em>Ruditapes philippinarum</em>) with a widespread distribution in the world, we assessed the impact of OAX on its embryonic and larval development as well as expressions of functional genes and lipids to indicate physiological and cellular performance. We found that embryonic development and larval shell formation were inhibited by OAX mainly due to the downregulation of key genes responsible for the uptake of calcium ions from ambient seawater (e.g., <em>NCX</em>, <em>VGCC</em> and <em>SERCA</em>) and the reduced production of bicarbonate ions through the catalytic action of carbonic anhydrase. In addition, a major remodelling in membrane lipids (e.g., PC, PE, PG, PI and PS) indicated that OAX impacted the fluidity and stability of cell membrane, hindering the uptake of calcification substrates. The depletion in energy reserves, such as triacylglycerol, can also account for the impairment in larval shell formation under OAX conditions. By integrating transcriptomics and lipidomics, our findings illustrate a novel molecular mechanism underlying the detrimental effect of OAX on larval development and hence population maintenance of marine organisms, which can have profound implications for sustaining ecosystem stability and aquaculture management.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101566"},"PeriodicalIF":2.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative biochemical, transcriptome and metabolome investigation on reproductive maturation of wild and captive female Monopterus albus","authors":"Hang Yang ,&nbsp;Zhen Xu ,&nbsp;Quan Yuan ,&nbsp;Weiwei Lv ,&nbsp;Weiwei Huang ,&nbsp;Yuning Zhang ,&nbsp;Wei Hu ,&nbsp;Xiao Wang ,&nbsp;Wenzong Zhou","doi":"10.1016/j.cbd.2025.101567","DOIUrl":"10.1016/j.cbd.2025.101567","url":null,"abstract":"<div><div>The main purpose of this study is to systematically elucidate mechanisms underlying ovarian development in wild and captive female <em>Monopterus albus</em> at the biochemical, transcriptomic, and metabolomic levels. Wild and captive female <em>M. albus</em> in Shanghai were continually collected over 1 year, and their ovarian development was analyzed statistically. We determined serum lipid metabolism and hormone contents of the fish during the study period, along with the largest differences in gonadosomatic indices (GSIs), and performed then transcriptome and metabolome detection analysis on their ovaries. The results demonstrated that wild group had approximately 1 month longer reproductive period than the captive group. Moreover, during the reproductive period, the wild group had higher GSIs than the captive group. Compared with the captive group, the wild group demonstrated significantly higher serum triglyceride, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, 17β-estradiol, and progesterone contents in serum. Transcriptome and metabolome analyses revealed that 2318 genes and 172 metabolites were upregulated in the wild group, whereas 662 genes and 223 metabolites were downregulated. Furthermore, integrated transcriptome and metabolome analyses revealed that cholesterol metabolism was significantly affected, indicating that it was the main pathway affecting ovarian development in both wild and captive female <em>M. albus</em>. In this pathway, 17 genes (including apolipoprotein A, lipoprotein lipase and sortilin) and 2 metabolites (taurocholic acid and taurochenodesoxycholic acid) were significantly upregulated. In general, ovarian development was better in wild female <em>M. albus</em> than farmed ones.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101567"},"PeriodicalIF":2.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-targeted metabolomics studies revealed the effects of environmentally relevant concentrations of perfluorooctane sulfonic acid (PFOS) on carbohydrate metabolism in red claw crayfish (Cherax quadricarinatus)","authors":"Shuang Hong ,&nbsp;Chengbin Liu ,&nbsp;Xiaoyu Liu ,&nbsp;Qinxiong Rao ,&nbsp;Xianli Wang ,&nbsp;Qicai Zhang ,&nbsp;Chunxia Yao ,&nbsp;Weiguo Song","doi":"10.1016/j.cbd.2025.101548","DOIUrl":"10.1016/j.cbd.2025.101548","url":null,"abstract":"<div><div>Perfluorooctane sulfonate (PFOS), a persistent organic pollutant, poses significant threats to aquatic ecosystems and organisms. This study investigated the biological effects of environmentally relevant PFOS concentrations (0 ng/L, 1 ng/L, 10 μg/L) on <em>Cherax quadricarinatus</em>, a freshwater crayfish of economic importance, focusing on histopathology, apoptosis (via TUNEL staining), and metabolomic alterations. The results showed that PFOS exposure caused tissue damage to the hepatopancreas of <em>C. quadricarinatus</em>, and PFOS exposure caused apoptosis of <em>C. quadricarinatus</em> hepatopancreas. In addition, the metabolomics results showed that there were several significant differential metabolites and metabolic pathways, which indicated that PFOS exposure can cause metabolic disorders in the hepatopancreas of <em>C. quadricarinatus</em>. The Starch and sucrose metabolism pathway was significantly enriched in both groups, suggesting the potential role of this pathway in the biological effects of PFOS on <em>C. quadricarinatus</em>. In addition, in 1 ng/L group, four significant differential metabolic pathways: Glucagon signaling pathway, Glycerolipid metabolism, Starch and sucrose metabolism, and Glycolysis/Gluconeogenesis were found, which demonstrated the adverse effects of PFOS even at environmental relevant concentration. Furthermore, in all the four metabolic pathways, glucose-1-phosphate is the only mutual significant differential metabolite, indicating that the carbohydrate metabolism might be the target affecting pathway and the glucose-1-phosphate assumed an important role in the metabolic effect of environmental relevant concentration of PFOS. This study revealed the effect of PFOS on <em>C. quadricarinatus</em> through tissue physiology and combined with metabolomics, which is of great significance for exploring the practical biological effects and health risk of PFOS in the actual aquatic environment.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101548"},"PeriodicalIF":2.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The molecular response of Neoseiulus bicaudus to cold acclimation","authors":"Siqiong Tang,&nbsp;Kaiqin Mu,&nbsp;Xinqi Liang,&nbsp;Jie Su,&nbsp;Jianping Zhang","doi":"10.1016/j.cbd.2025.101565","DOIUrl":"10.1016/j.cbd.2025.101565","url":null,"abstract":"<div><div><em>Neoseiulus bicaudus</em> is a beneficial predatory mite used for the control of spider mites. Temperature is a crucial factor that influences the distribution, growth, and development of <em>N. bicaudus</em>. Cold acclimation is an important arthropod strategy used to improve cold tolerance. We investigated the impact of cold acclimation on the cold tolerance of <em>N. bicaudus</em>. To gain insights into the molecular mechanisms underlying cold acclimation of <em>N. bicaudus</em>, we conducted transcriptome and proteomic analyses on three cold-acclimated groups (6-h: 3 °C for 6 h; 24-h: 3 °C for 24 h; 7-day: 9 °C for 7 d). Cold acclimation, especially in the 7-day treatment, significantly improved the survival time of <em>N. bicaudus</em> at an acute low temperature (−6 °C). Multi-omics analysis revealed that cold acclimation in <em>N. bicaudus</em> involves coordinated regulation of genes and proteins related to energy metabolism and cellular protection. Cold acclimation suppressed energy-intensive pathways like fatty acid synthesis and glycolysis, reducing energy expenditure. However, it enhanced expression of proteins in fatty acid oxidation, tricarboxylic acid cycle, and oxidative phosphorylation pathways to maintain energy balance. Moreover, cold acclimation upregulated genes and proteins involved in mRNA processing, transport, translation regulation, protein folding, and degradation, ensuring rapid repair and synthesis of proteins for homeostasis. RNA interference of NbHSP70 and NbHSP90 showed that these genes play a vital role in regulating the cold tolerance of <em>N. bicaudus</em>. These findings provide valuable resources and opportunities to uncover molecular acclimation mechanisms that support cold tolerance in Phytoseiid mites.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101565"},"PeriodicalIF":2.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}