Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Analysis of miRNA expression profiles during spermatogenesis of plateau zokor (Eospalax baileyi) under androgen antagonism treatment","authors":"Kejie Ou ,&nbsp;Kang An ,&nbsp;Biaobiao Hao ,&nbsp;Jia Kang ,&nbsp;Junhu Su","doi":"10.1016/j.cbd.2025.101636","DOIUrl":"10.1016/j.cbd.2025.101636","url":null,"abstract":"<div><div>Seasonal reproduction in animals is characterized by programmed activation, dormancy of reproductive activities, and precise regulation of hormone levels. Androgens secreted by Leydig cells are essential for testicular development and spermatogenesis. To explore the mechanism of androgen in spermatogenesis in seasonal breeding animals, this study used the plateau zokor (<em>Eospalax baileyi</em>) as a model and, during the breeding season, treated it with the androgen receptor antagonist, flutamide, to analyze its effect on the microRNA (miRNA) expression profile in testicular tissue. Flutamide treatment resulted in marked changes in the expression of 91 miRNAs in the testes (28 upregulated and 63 downregulated), including 44 known and 47 newly predicted miRNAs. Functional enrichment analysis of the target genes of differentially expressed miRNAs demonstrated significantly enriched cell adhesion, hypoxia response, apoptosis process, inflammatory response， lipid metabolism (Gene Ontology analysis, <em>P</em> &lt; 0.05) and ferroptosis, fatty acid metabolism and other signaling pathways (Kyoto Encyclopedia of Genes and Genomes analysis, <em>P</em> &lt; 0.05). Based on this, an interaction network of miRNA–mRNA in the spermatogenesis and ferroptosis pathways was constructed. Dual-luciferase reporter gene assay confirmed that <em>ACSL4</em> was a direct target gene of miR-486-y. The expression of miR-486-y was inhibited by an antagomir, which verified its involvement in the regulation of spermatogenesis in the plateau zokor during reproduction. Androgens regulate spermatogenesis in plateau zokors by inhibiting specific miRNAs (such as miR-486-y). This study provides molecular evidence and new perspectives on the regulatory network of seasonal reproduction in animals.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101636"},"PeriodicalIF":2.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into Bacillus sp. KNSH11 from Litopenaeus vannamei intestine: Probiotic potential, safety, and aquaculture applications","authors":"Waraphorn Sihamok ,&nbsp;Sk Injamamul Islam ,&nbsp;Luu Tang Phuc Khang ,&nbsp;Orathai Dangsawat ,&nbsp;Papungkorn Sangsawad ,&nbsp;Truong Anh Tu ,&nbsp;Cao Phuong Thao ,&nbsp;Nguyen Dinh-Hung ,&nbsp;Patima Permpoonpattana ,&nbsp;Nguyen Vu Linh","doi":"10.1016/j.cbd.2025.101633","DOIUrl":"10.1016/j.cbd.2025.101633","url":null,"abstract":"<div><div>In the context of sustainable aquaculture, probiotics represent a promising alternative to antibiotics for promoting shrimp health and disease resistance. In this study, <em>Bacillus</em> sp. KNSH11, a Gram-positive, rod-shaped bacterium isolated from the intestine of whiteleg shrimp (<em>Litopenaeus vannamei</em>), was characterized to assess its probiotic potential. The strain exhibited excellent sporulation efficiency (&gt; 99 %), supporting its resilience under harsh environmental conditions. Functional assays demonstrated that KNSH11 retained high viability under various stressors, including acidic pH (2–4), bile salts, elevated temperatures (up to 95 °C), and lysozyme exposure, indicating robust tolerance to gastrointestinal and processing challenges. Metabolic profiling revealed substantial lactic acid production with minimal levels of acetate and propionate, distinguishing it from conventional lactic acid bacteria. The strain also exhibited strong antioxidant activity and moderate antibiofilm effects against pathogenic bacteria. Antibiotic susceptibility testing showed sensitivity to amoxicillin, chloramphenicol, kanamycin, and tetracycline (all at 30 μg/disc), while resistance was observed against ampicillin and penicillin (10 μg/disc each). Whole genome sequencing confirmed the absence of virulence genes and identified the presence of mobile genetic elements, a CRISPR/Cas system, and gene clusters potentially responsible for bacteriocin production. Collectively, these results indicate that <em>Bacillus</em> sp. KNSH11 exhibits key probiotic characteristics and genomic features consistent with a safe profile, supporting its potential application in sustainable shrimp aquaculture, pending further in vitro and in vivo validation.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101633"},"PeriodicalIF":2.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel indel-based molecular sex identification system for oplegnathus fasciatus: Insights from ndc80 gene polymorphisms and aquaculture applications","authors":"Yongshuang Xiao ,&nbsp;Pingrui Xu ,&nbsp;Jun Li ,&nbsp;Zhizhong Xiao","doi":"10.1016/j.cbd.2025.101632","DOIUrl":"10.1016/j.cbd.2025.101632","url":null,"abstract":"<div><div><em>Oplegnathus fasciatus</em> is a commercially important marine fish species, valued both in wild fisheries and aquaculture. It possesses a multivalent sex-determination system (X₁X₁X₂X₂/X₁X₂Y) and exhibits marked sexual growth dimorphism, with males demonstrating significantly faster growth rates. Sex-specific molecular markers are instrumental in advancing selective breeding strategies. In this study, whole-genome screening of <em>O. fasciatus</em> revealed a male-specific structural variant within an intronic region of <em>ndc80</em>: two insertions of 3<!--> <!-->bp and 538<!--> <!-->bp (totaling 541<!--> <!-->bp), which were consistently absent in females. To facilitate practical application, we developed a PCR-based assay using a single primer pair that amplifies a conserved region flanking the insertion site. This assay reproducibly generates distinct banding patterns: males yield two fragments (208<!--> <!-->bp and 749<!--> <!-->bp), consistent with the 541<!--> <!-->bp male-specific insertion, whereas females yield only the 208<!--> <!-->bp amplicon. This method enables efficient, high-throughput sex identification in <em>O. fasciatus</em> without the need for sequencing. Using standard agarose gel electrophoresis, the assay reliably distinguishes sexes through clearly divergent banding patterns. Beyond applications in selective breeding, these sex-specific markers provide critical molecular insights into the sex determination mechanisms and the genetic basis of sexual dimorphism in <em>O. fasciatus</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101632"},"PeriodicalIF":2.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145076745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and sex-biased expression analysis of olfactory-related genes in Lymantria dispar based on transcriptome and qRT-PCR","authors":"Lina Yang ,&nbsp;Dun Jiang ,&nbsp;Weichao Ma ,&nbsp;Shanchun Yan","doi":"10.1016/j.cbd.2025.101634","DOIUrl":"10.1016/j.cbd.2025.101634","url":null,"abstract":"<div><div><em>Lymantria dispar</em>, a destructive forest pest, poses a significant threat to forest ecosystems. Olfaction plays a pivotal role in mate finding, host location, and oviposition site selection in insects. This study aimed to systematically characterize olfactory-related genes in adult <em>L. dispar</em> and examine sex-specific expression profiles, shedding light on the molecular mechanisms of olfactory perception in this species. Antennal transcriptomes of male and female <em>L. dispar</em> were sequenced, generating 36.61 Gb of high-quality clean data. A total of 134 olfactory-related genes were identified, including 40 odorant receptors (ORs), 12 gustatory receptors (GRs), 20 ionotropic receptors (IRs), 17 chemosensory proteins (CSPs), 38 odorant-binding proteins (OBPs), 3 odorant-degrading enzymes (ODEs), and 4 sensory neuron membrane proteins (SNMPs). Relative expression levels of these genes were validated by qRT-PCR, revealing that 28 genes were significantly upregulated in female antennae, while 32 genes exhibited higher expression in males. These findings aligned closely with the transcriptome data. This study provides a comprehensive characterization of olfactory-related genes in <em>L. dispar</em> antennae and uncovers their sex-biased expression, offering valuable insights into the molecular mechanisms of pheromone detection and other olfactory-driven behaviors in this pest species.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101634"},"PeriodicalIF":2.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroendocrine transcriptomics in Eriocheir sinensis: Photoperiod-induced modulation of gonadal development and circadian clock genes in neural tissues","authors":"Gaoyuan Yuan ,&nbsp;Meihui Huo ,&nbsp;Boyi Zheng ,&nbsp;Zhichao Wang ,&nbsp;Xugan Wu ,&nbsp;Meimei Liu ,&nbsp;Zhiguo Dong","doi":"10.1016/j.cbd.2025.101629","DOIUrl":"10.1016/j.cbd.2025.101629","url":null,"abstract":"<div><div>Photoperiod is a critical environmental cue that orchestrates reproductive physiology and circadian biology in crustaceans, yet the neural gene networks linking photoperiod to ovarian maturation and clock regulation remain largely unknown. To elucidate the molecular mechanisms underlying photoperiod regulation of ovarian development and associated circadian rhythmicity, the eyestalk (SG) and brain ganglion (BG) of female Chinese mitten crab (<em>Eriocheir sinensis</em>) from two photoperiod groups (Light (L): Dark (D) = 0 h: 24 h (L0) and L: D = 18 h: 6 h (L18)) were used for transcriptome analysis. The analysis identified 298 differentially expressed genes (DEGs) in the brain and 109 DEGs in the eyestalk. KEGG enrichment analysis indicated these DEGs were primarily associated with ovarian development and circadian rhythm-related signaling pathways. Key ovarian development-related genes, including E3 ubiquitin-protein ligase lubel-like isoform X6, 5-hydroxytryptamine receptor-like, serine/threonine-protein phosphatase alpha-2, estrogen sulfotransferase, cytochrome P450 2 L1-like, follicle-stimulating hormone receptor-like isoform X1 and E3 ubiquitin-protein ligase CHIP (EULCHIP), exhibited significant upregulation or downregulation in the L18-BG group. Meanwhile, genes such as EULCHIP, E3 ubiquitin-protein ligase TRIM71-like, CREB-binding protein-like isoform X2, serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C, collagen alpha-5(IV) chain, integrin beta 1, ecdysone-induced protein 74EF-like, and insulin-like growth factor-binding protein complex acid labile subunit showed significant upregulation or downregulation in the L18-SG group. These findings suggest that prolonged photoperiods enhance ovarian development and modulate reproductive endocrine activity during ovarian maturation. Furthermore, circadian rhythm-related genes such as period circadian protein-like isoform X1, prostaglandin D synthase, and acetylcholine receptor subunit alpha-type acr-16 displayed marked differential expression between photoperiod groups, indicating disrupted molecular oscillations and gradual desynchronization of circadian rhythmicity under extended light exposure. This study provides critical, systems-level insights into the photoperiod-driven regulatory networks of ovarian development and endocrine dynamics in crustaceans, offering a molecular basis for optimizing aquaculture practices and advancing our understanding of crustacean reproductive physiology.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101629"},"PeriodicalIF":2.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating imidacloprid resistance in Amrasca biguttula biguttula (Ishida) (Hemiptera: Cicadellidae): Insights from RNA-Seq and functional validation using RT-qPCR","authors":"Muthugounder Mohan ,&nbsp;B.R. Basavaarya ,&nbsp;Karuppannasamy Ashok ,&nbsp;Sathasivam Malarvizhi ,&nbsp;P.J. Aneesha ,&nbsp;Gandhi R. Gracy ,&nbsp;Thiruvengadam Venkatesan ,&nbsp;R.S. Ramya ,&nbsp;S.N. Sushil","doi":"10.1016/j.cbd.2025.101630","DOIUrl":"10.1016/j.cbd.2025.101630","url":null,"abstract":"<div><div>The increasing challenge in agricultural insect pest management is the rapid development of insecticide resistance. <em>Amrasca biguttula biguttula</em>, a significant sap-sucking pest of cotton and other crops, causes severe damage through feeding and toxin injection while exhibiting resistance to multiple insecticides, including Imidacloprid. Understanding the genetic factors and mechanisms driving resistance in <em>A. biguttula biguttula</em> is crucial for effective pest control. This study utilized Illumina HiSeq sequencing to generate a transcriptome assembly and analyze differential gene expression in response to Imidacloprid. Differential expression analysis using DESeq2 identified 177 significantly expressed transcripts, with 67 upregulated and 110 downregulated. To validate these findings, the gene expression of selected genes in response to Imidacloprid exposure at LC₅₀ concentration (24.91 ppm) was tested at different time points (6 h, 12 h, and 24 h) relative to 0 h (control). The significant upregulation of detoxification-related genes such as cytochrome P450 monooxygenase (CYP303A1, CYP4DE1, CYP3184-fragment1 and CYP3939A2), three carboxylesterases (CE-1, CE-2, and CE-3), and one ABC transporter (ABC), highlights its potential role in Imidacloprid resistance. This study is the first to explore the molecular mechanisms underlying Imidacloprid resistance in <em>A. biguttula biguttula</em> and offers valuable insights for improving current pest management strategies.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101630"},"PeriodicalIF":2.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood meal modulates midgut bacterial community structure and metabolic function in Aedes albopictus","authors":"Xiao Wei ,&nbsp;Xiangna Zhao","doi":"10.1016/j.cbd.2025.101628","DOIUrl":"10.1016/j.cbd.2025.101628","url":null,"abstract":"<div><div>The transmission of mosquito-borne diseases is intrinsically linked to mosquito blood-feeding behavior, yet the metabolic adaptations of the midgut microbiota in response to blood meals remain poorly understood. This study aimed to characterize the structural and functional changes in the midgut microbiota of <em>Aedes albopictus</em> following blood feeding and to elucidate their potential physiological implications. In this study, we employed 16S rRNA gene amplification coupled with PacBio Sequel II sequencing to characterize shifts in the midgut microbiota of <em>Aedes albopictus</em> before and after blood feeding on mice. Following blood feeding, we observed a significant restructuring of the microbial composition. This shift was characterized by a marked enrichment of <em>Acinetobacter</em> and <em>Wolbachia</em>, with <em>Wolbachia</em> displacing <em>Flavisolibacter</em> as the dominant taxon. Functionally, blood feeding promoted the upregulation of pathways related to mobile genetic elements and stress tolerance, largely driven by <em>Lactobacillaceae</em>. Furthermore, we presented the first comprehensive analysis of blood meal-induced metabolic network remodeling in the mosquito midgut microbiota. Post-prandial microbiota exhibited enhanced metabolic capacity for pyruvate and glycine catabolism. These findings reveal that blood meals induce rapid microbial metabolic adaptation aimed at nutrient utilization and oxidative management. This study provides insight into how microbiota dynamics support mosquito host adaptation under nutritional stress and offers potential targets for microbiome-based strategies to interfere with vector competence.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101628"},"PeriodicalIF":2.2,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of hypoxia and thermal stress on hemocyte response in Pacific oysters (Crassostrea gigas): Insights from transcriptomic and proteomic analyses","authors":"Jeong-Hwa Kim ,&nbsp;Nobuhisa Kajino ,&nbsp;Jong-Seop Shin ,&nbsp;Hyun-Sung Yang ,&nbsp;Hee-Jung Lee ,&nbsp;Kwang-Sik Choi ,&nbsp;Hyun-Ki Hong","doi":"10.1016/j.cbd.2025.101626","DOIUrl":"10.1016/j.cbd.2025.101626","url":null,"abstract":"<div><div>Hypoxia and elevated seawater temperatures are increasingly prevalent stressors in marine ecosystems, significantly impacting the physiology of marine organisms. This study investigates the transcriptomic and proteomic responses of Pacific oyster (<em>Crassostrea gigas</em>) hemocytes to hypoxia alone (water temperature, 23 °C; dissolved oxygen [DO] level, 1 mg O₂/L) and combined hypoxia with high temperature (water temperature, 28 °C; DO level, 1 mg O₂/L) over a 10-day exposure period. Using RNA sequencing and liquid chromatography-mass spectrometry, we identified distinct molecular responses to these stressors. Hypoxia alone upregulated genes associated with DNA replication and protein localization, while suppressing those involved in metabolic activity, reactive oxygen species (ROS) generation, and phagosome function. Under combined hypoxia and high temperature stress, these effects were more pronounced, with enhanced downregulation of hydrolase activity and tyrosine metabolism, indicating synergistic impacts on oyster physiology. Notably, prostaglandin (PG) synthesis was specifically induced under combined stress conditions, suggesting its potential as a biomarker for environmental stress. These findings highlight how hypoxia and thermal stress interact to compromise immune function and energy metabolism in <em>C. gigas</em>, providing critical insights into the physiological vulnerabilities of commercially important bivalves under climate change.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101626"},"PeriodicalIF":2.2,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure of crayfish (Procambarus clarkii) to pinoxaden disrupts hepatic transcriptome, gut microbiome, and redox homeostasis","authors":"Ruichun Li ,&nbsp;Wenqi Qian ,&nbsp;Chenhui Li ,&nbsp;Boxuan Yao ,&nbsp;Aimin Wang ,&nbsp;Tao Guan ,&nbsp;Zhicen Liu ,&nbsp;Lei Gao ,&nbsp;Dongli Qin","doi":"10.1016/j.cbd.2025.101627","DOIUrl":"10.1016/j.cbd.2025.101627","url":null,"abstract":"<div><div>With the widespread use of pesticides, pinoxaden has become a commonly used herbicide in rice-crayfish co-culture fields. However, the mechanism of toxicity to aquatic organisms is unclear. This study investigated the acute toxicity effects of pinoxaden on crayfish. Biochemical analysis revealed significant alterations in malondialdehyde content, as well as glutathione peroxidase, superoxide dismutase and catalase activities in hepatopancreas tissues following pinoxaden exposure. Histological examination revealed pinoxaden-induced impairment in the hepatopancreas and gut tissues of crayfish. Transcriptomic profiling detected genes with differential expression following pinoxaden treatment, significant enrichment of pathways including lysosome, antigen processing presentation, phagosome. Furthermore, pinoxaden exposure altered the abundance of bacterial families such as Actinobacteriota and <em>Erysipelatoclostridium</em> in the gut microbiota of exposed groups. This study provides important insights into the toxicity of pinoxaden to crayfish, offering practical guidance for the scientifically based and rational use of pesticides in rice-crayfish co-culture systems.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101627"},"PeriodicalIF":2.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative venom proteomes of Malaysian snake species from regions in Southeast Asia obtained by shotgun proteomics: A systematic review","authors":"Shi Yuin Chong,&nbsp;Xin Yee Lee,&nbsp;Syafiq Asnawi Zainal Abidin","doi":"10.1016/j.cbd.2025.101624","DOIUrl":"10.1016/j.cbd.2025.101624","url":null,"abstract":"<div><div>Snakebite envenoming (SBE) is a critical yet neglected public health issue in ASEAN countries, including Malaysia. Antivenom immunotherapy is the primary treatment for SBE, functioning by neutralising toxic venom components through antibodies raised from immunised animals. Current antivenoms come with potential reduced efficacy due to regional venom variability. The aim of this systematic review is to consolidate and compare the venom proteome composition from Malaysian venomous snakes (elapids and viperids) sampled from Malaysia, with their counterparts sampled across other Southeast Asia (SEA) regions. Literature search on ScienceDirect, PubMed, and Ovid MEDLINE databases were done on relevant studies published in English from Jan 2014 to Dec 2024. A total of 630 studies were imported for screening. 68 articles received full text assessment to determine their eligibility, and finally, 36 articles were eligible for the systematic review. Among these, proteomic compositions from 9 elapid and 5 viperid species from Malaysia, and their counterparts in other SEA regions were consolidated, with 3 studies investigated both elapids and viperids, 26 studies investigated only elapid(s), and 7 studies investigated only viperid(s). Results revealed geographical variability between venom proteomes at the inter- and intra- specific level. Comparative analysis of venom proteomes between Malaysian venomous snakes and their counterparts located across SEA enables correlation between intra-species variation and clinical toxinology, supporting efforts towards the development of a more effective antivenom (i.e., species- and region-specific, as well as 'universal'), and providing valuable information for clinicians in administering a more appropriate antivenom.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101624"},"PeriodicalIF":2.2,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}